bacteria in bile of patients with bile duct inflammation.bile samples taken intraoperatively from 100 patients with three different bile system diseases were subjected to bacteriological analysis. statistically significant differences between the types of aerobic and anaerobic bacteria present in the bile were found.19873436318
[studies on antimicrobial concentrations of flomoxef in serum, pelvic dead space exudate, and pelvic organs/tissues].to women undergoing radical and total hysterectomy, flomoxef (fmox, 6315-s) in a dose of 2 g was administered by intravenous drip infusion over 1 hour and drug concentrations in serum and pelvic dead space exudate as well as pelvic organs/tissues were determined over time. the following results were obtained: 1. serum concentrations of fmox after intravenous infusion showed the peak value of 92.86 +/- 17.05 micrograms/ml at the end of infusion and then gradually decreased to 29.00 +/- 10.49 micr ...19873444023
anaerobic bacteraemia. 19873449455
[fundamental and clinical studies of imipenem and imipenem/cilastatin sodium in the field of obstetrics and gynecology].fundamental and clinical studies of imipenem (mk-0787), a new type of carbapenem antibiotic, and mk-0787 combined with cilastatin sodium (mk-0791), a renal dipeptidase inhibitor, were carried out. the results obtained were as follows: mk-0787 500 mg alone or mk-0787 500 mg with mk-0791 500 mg was administered by intravenous drip infusion over 30 minutes. plasma levels of the drug were similar either following the administration of 500 mg of mk-0787 alone or 500 mg of mk-0787 with 500 mg of mk-07 ...19863463787
severe infections of the head and neck resulting from gas-forming organisms: report of case.a case of severe infection of the head and neck associated with gas-forming organisms in a patient with fractures of the mandible is presented. selected aspects of antibiotic therapy are discussed.19873468169
comparative serum bactericidal activity against test anaerobes in volunteers receiving imipenem, clindamycin, latamoxef and metronidazole.ten healthy volunteers received on separate days the following regimens: imipenem 500 mg, clindamycin 600 mg, latamoxef 1 g, and metronidazole 500 mg. the antibiotics were given intravenously as an infusion over 15 min. blood samples were obtained before and 30 min, 1 and 6 h after the start of the infusion. serum bacteriostatic and bactericidal activities were measured against the following strains of strict anaerobes: two strains of bacteroides fragilis, one strain each of b. vulgatus, b. thet ...19873471750
effects of extracellular products of a presumed gingival pathogen, the bacterium peptostreptococcus 84h14s, on cultured human fibroblasts and hela cells.culture fluids from this microorganism, fibroblasts and hela cells contained potent factors which inhibited thymidine uptake in hela cells. the outcome of heating the culture fluid and fractionating it by gel filtration suggested that this was not due to bacterial hyaluronidase activity. purified peptostreptococcal hyaluronidase and several commercially-available mucopolysaccharidases also did not inhibit thymidine uptake.19873478031
[peptostreptococcus sepsis complicating third molar pericoronitis]. 19873483065
in vitro activity of flurithromycin, a novel macrolide antibiotic.flurithromycin is an (8,s)-8-fluoroerythromycin isolated from the fermentation broth of streptomyces erythraeus atcc 31772, a blocked mutant of a strain producer of erythromycin. its in vitro antibacterial activity has been determined on recent clinical isolates of respiratory pathogens. the range of mic for streptococcus pneumoniae and streptococcus beta-haemolyticus group a is from 0.0015 to 0.006 microgram/ml, for haemophilus influenzae from 0.012 to 0.4 microgram/ml, for staphylococcus aureu ...19863487389
[in vitro susceptibilities of brl 25000 (clavulanic acid-amoxicillin) against causative organisms in the field of obstetrics and gynecology].the in vitro susceptibilities of various causative organisms recently isolated from patients with genital infections to brl 25000 (a formulation with 2 parts of amoxicillin and 1 part of potassium clavulanate), amoxicillin (ampc), cefaclor (ccl), cephalexin (cex), cefadroxil (cdx) and cefroxadine (cxd) were determined. beta-lactamase-producing strains were detected by the nitrocefin disc method. frequencies of isolation of beta-lactamase producing strains of e. coli, k. pneumoniae and b. fragili ...19863488425
the intraocular environment and experimental anaerobic bacterial endophthalmitis.anaerobic bacteria are prevalent in conjunctival flora but have not been adequately investigated as possible causes of endophthalmitis. the mean oxidation-reduction potential (eh) of the rabbit vitreous was found to be +25.1 mv, well within the limiting eh value of many anaerobes. there was an oxygen pressure gradient in the vitreous ranging from 2.1 mm hg immediately posterior to the lens to approximately 20 mm hg adjacent to the medullary ray. endophthalmitis was produced with pure cultures of ...19873499883
microbiological studies of tracheostomy site wounds.specimens were obtained from 25 patients who developed tracheostomy site wound infections. aerobic bacteria only were isolated in 4 (16%) instances, anaerobic bacteria only in 2 (8%), and mixed aerobic and anaerobic isolates were recovered in 19 (76%). a total of 145 isolates (72 aerobes and 73 anaerobes) were recovered, an average of 5.8 isolates per specimen (2.9 aerobes and 2.9 anaerobes). the most frequently recovered isolates were peptostreptococcus sp., bacteroides sp., alpha-hemolytic str ...19873502089
intraventricular haemorrhage complicating a brain abscess.intraventricular haemorrhage occurred in a patient with a parietal rim-enhancing mass on computed tomographic scan. at operation a brain abscess was identified and removed. peptostreptococcus and fusobacterium were isolated, possibly of dental origin. the possible sources of this intracranial bleeding are discussed. a neoplasm should not always be considered in the case of a cerebral ring-enhancing mass complicated with intracranial bleeding; in selected cases, brain abscess should be excluded t ...19873502181
[strict anaeroba in the endodontic region]. 19863506338
[sensitivity of anaerobes to 8 antibiotics].we studied the susceptibility of 282 obligate anaerobes to 8 antibiotics. the strains were isolated from clinical specimens taken during 1983 and 1984. minimal inhibitory concentrations (mics) were determined using a microplate method for 245 strains and agar dilution for 33 bacteroides asaccharolyticus strains. tested strains were as follows: 85 bacteroides fragilis, 71 clostridium, 19 fusobacterium nucleatum, 35 peptostreptococcus, 39 bacteroides oralis and bacteroides bivius and 33 bacteroide ...19863534760
pseudofolliculitis barbae with keloids.pseudofolliculitis barbae is a common cutaneous infection occurring in the bearded area of the face of black men. the infection is caused by ingrown hairs that produce an inflammatory foreign body reaction characterized by papules and pustules at the point of hair penetration that may result in permanent scarring, usually in grooved patterns, and occasionally in keloids. the purpose of this article is to describe the successful treatment of a patient with pseudofolliculitis with keloids that was ...19863540094
pathogenicity and therapy of anaerobic bacteria in upper respiratory tract infections. 19873547288
use of intravenous ciprofloxacin in difficult-to-treat infections.intravenous ciprofloxacin was administered to 54 patients who were either critically ill or in whom oral administration was not possible. the 31 males and 23 females ranged in age from 20 to 89 years (mean, 53.2 +/- 17.8 years). patients had "difficult-to-treat" infections, i.e., respiratory infections (15), abscesses (four intraabdominal, three lung, two soft tissue, and one intrahepatic), deep soft tissue infections (10), chronic post-traumatic osteomyelitis in exacerbation (nine), upper urina ...19873555059
comparative activity of metronidazole and tinidazole against clostridium difficile and peptostreptococcus anaerobius.broth dilution mics of metronidazole against 38 strains of clostridium difficile (0.13 microgram/ml) and 11 strains of peptostreptococcus anaerobius (0.11 microgram/ml) were lower than those of tinidazole (0.16 and 0.17 microgram/ml, respectively). as molar concentrations the nitroimidazoles were equally active. the mics of the two drugs correlated positively, and the relative activity of tinidazole increased with decreasing susceptibility of both species.19873566248
increased resistance towards two systemic experimental infections by tetrachlorodecaoxygen anion complex. possible implications of cellular and humoral immunity.the tetrachlorodecaoxygen anion complex (tcdo, oxoferin) given intravenously 1 h after intravenous infection of mice with candida albicans increased the host's resistance gradually in doses up to 3.1 mumol/kg body weight (b.w.). above this level, the stimulatory effect decreased, turning to an inhibition at a dose of 18.6 mumol/kg b.w. tcdo. repeated applications of the optimum single dose had no cumulative effect. in experimental infections with the strictly anaerobic peptostreptococcus interme ...19863566839
viability of lyophilized anaerobes in two media.viability of 15 species of anaerobes was followed after freeze-drying and storage for 1 year. organisms maintained in a 12% sucrose concentration in chopped meat carbohydrate broth survived longer and maintained higher viability counts than those organisms in double-strength skim milk.19873568744
heparinase production by anaerobic bacteria.the production of heparinase by a wide range of anaerobic bacteria isolated from clinical specimens was investigated. none of the 29 strains of bacteroides fragilis produced heparinase. of 62 other bacteroides tested, only two of four strains of b ovatus, two of three strains of b thetaiotaomicron, and two of four strains of b uniformis were heparinase producers. none of the 48 strains of fusobacteria or seven strains of veillonella produced heparinase. the anaerobic cocci (19 peptococci and sev ...19873584480
rapid identification of obligately anaerobic gram-positive cocci using high-performance liquid chromatography.high-performance liquid chromatography was evaluated as a rapid means of identifying obligately anaerobic gram-positive cocci of medical interest. isolates were inoculated into a defined chemical medium consisting primarily of amino acids and were incubated aerobically for 1 h at 35 degrees c. after removal of organisms, the supernatant fluids were derivatized for 1 min at room temperature by the addition of o-phthalaldehyde. the total time required to run a chromatogram was approximately 50 min ...19873597760
gastric acidity: an important factor regulating the composition of the bacterial flora in the small intestine.the role of gastric acidity in regulating the bacterium composition in the small intestine was studied. gastric acidity was determined with the kay test. intestinal juice obtained by a probe was examined for the presence and number of microorganisms. parallel with the increase in gastric acidity, the number of bacteria in the jejunal juice generally decreased. their composition also varied: in anacid patients e. coli predominated whereas in those with free hydrochloric acid, streptococcus alpha ...19863601581
increased faecal glycosidases in patients with crohn's disease.the strictly anaerobic faecal flora of patients with crohn's disease (cd) contains higher numbers of coccoid rods (eubacterium, peptostreptococcus and coprococcus species) and gram-negative rods (bacteroides vulgatus) than the flora of healthy subjects. the abnormal flora of patients with cd might affect levels of glycolytic enzymes and metabolic products and thus play some role in the pathogenesis of cd. therefore, the activity of 23 glycosidases that can degrade mucus glycoproteins or plant po ...19873609504
controlled evaluation of modified radiometric blood culture medium supplemented with gelatin for detection of bacteremia and fungemia.although the addition of 1.2% gelatin to broth blood culture media containing sodium polyanetholesulfonate has been shown to enhance detection of certain bacteria, including neisseria meningitidis, n. gonorrhoeae, peptostreptococcus anaerobius, and gardnerella vaginalis, the effect of such supplementation on the detection of other microorganisms causing bacteremia and fungemia is not known. therefore, we studied bactec 6b medium with and without gelatin in 6,833 paired comparisons to examine the ...19873624437
[anaerobic bacteria in various infections and their antibiotic susceptibility patterns].in this study we have examined antibiotic susceptibility of anaerobic bacteria isolated from various samples of 75 patients. anaerobic bacteria from 22 (29.3%) patients, and only aerobic bacteria from 29 (38.7%) patients have been isolated; there has been no growth in cultures from 24 (32%) patients. of 75 isolates from 51 patients 38 (50.6%) are anaerobic and 37 (49.4%) are aerobic bacteria. following anaerobic bacteria have been isolated: peptostreptococcus (13), peptococcus (5), microaerophil ...19863657617
[clinical studies on cefuzonam in obstetrics and gynecological infections].to evaluate the usefulness and the safety of cefuzonam (czon, l-105) against obstetric and gynecological infections, we conducted clinical trials and obtained the results summarized as follows. 1. thirteen patients with obstetric and gynecological infections (intrauterine infection 2 cases, adnexitis 7, 1 case each of abscess of adnexa uteri, abscess of the vaginal wall, pyelonephritis, and mammitis) were treated with czon. the czon was administered by intravenous injection or intravenous drip i ...19873669283
identification of anaerobic bacteria using high-performance liquid chromatography.20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ...19873673341
interactions of ciprofloxacin with clindamycin, metronidazole, cefoxitin, cefotaxime, and mezlocillin against gram-positive and gram-negative anaerobic bacteria.a total of 598 clinical isolates of anaerobic bacteria were tested against ciprofloxacin by the agar dilution technique with 10(5) cfu on wilkins-chalgren medium. selected strains representative of the six major genera of anaerobes relatively resistant to the quinolones were tested for interactions with ciprofloxacin in combination with clindamycin, metronidazole, cefoxitin, cefotaxime, or mezlocillin by using a checkerboard agar dilution technique. cefotaxime-ciprofloxacin and clindamycin-cipro ...19873674848
enrichment of subgingival microflora on human serum leading to accumulation of bacteroides species, peptostreptococci and fusobacteria.this study was undertaken to identify ecological factors that favour opportunistic pathogenic species in the subgingival microflora. in a first approach, human serum as a substitute for gingival exudate, was used for batch-wise enrichment of subgingival plaque. the microflora resulting after 5-6 enrichment steps consisted of black-pigmented and non-black-pigmented bacteroides species, peptostreptococcus micros and fusobacterium nucleatum as the main organisms. it is noted that the same group of ...19873674857
the in-vitro activity of doxycycline and minocycline against anaerobic bacteria.the likelihood of bacterial resistance now prevents the use of oxytetracycline in the empirical therapy of anaerobic infections. this study investigates the in-vitro activity of two semi-synthetic derivatives, doxycycline and minocycline, against a range of anaerobic bacteria. mics for each antibiotic were determined by an agar incorporation technique. doxycycline and minocycline were four to eight times more active against the majority of strains than oxytetracycline. with the exception of bact ...19873680075
in vitro activity of ampicillin plus sulbactam against anaerobes compared to ampicillin and cefoxitin.the antimicrobial susceptibility of 195 recent clinical isolates of anaerobic bacteria was studied to ampicillin alone, ampicillin + 1 mg/l sulbactam, ampicillin + 5 mg/l sulbactam, and cefoxitin by means of agar dilution tests. the ampicillin-sulbactam combinations were the most effective drugs against species of the bacteroides fragilis group, the mic90 of ampicillin + 5 mg/l sulbactam for b. fragilis being less than 1 mg/l, compared to 256 mg/l of ampicillin, 4 mg/l of ampicillin + 1 mg/l sul ...19873692611
comparative activity of sch 34343 against anaerobic bacteria.the in vitro activity of sch 34343 was compared with that of imipenem, clindamycin, cefoxitin, and metronidazole against 78 strains of anaerobic bacteria in the presence and absence of blood. wilkins-chalgren agar was used. sch 34343 and imipenem were the most active agents, inhibiting all strains at less than or equal to 8 micrograms/ml. the addition of blood had little effect on the activity of any of the agents. seven strains were unable to grow on wilkins-chalgren agar. even with the additio ...19863698546
[study of concentrations of clindamycin phosphate in bone marrow blood and tissue].clindamycin phosphate (cldm) was administered to 33 surgical cases in orthopedics to treat or prevent infection (600 mg in 15 cases and 1,200 mg in 18 cases by intravenous drip infusion over 60 minutes) and the concentrations of cldm in the bone marrow blood and tissue of the operative field as well as in venous blood were determined with the cup plate method of bioassay by collecting specimens immediately after completion of infusion or 30 or 60 minutes later. the concentration of cldm in bone ...19863702055
immunochemical characterization of the cell surface carbohydrate antigens of peptostreptococcus anaerobius.two carbohydrate antigens were isolated from the cell surface of peptostreptococcus anaerobius. one, extracted from purified cell walls with naoh, contained glucose and phosphorus, with traces of galactosamine and glucosamine. serological activity was detected by a 'dot blot' procedure. the second antigen, extracted from cell membranes with phenol and purified by chromatography on sepharose 6b and an immunoadsorbent column, contained glucose, glycerol phosphate, phosphorus and fatty acids. antig ...19863711862
microbiologic analyses of nasal polyp tissue.nasal polyps from 40 patients were cultured within 2 1/2 hours after surgical removal to determine whether microorganisms were present. the first 20 polyps were cultured for aerobic and anaerobic bacteria, viruses, fungi, mycoplasmas, and mycobacteria. of these 20 polyps, eight were sterile by all tests, one grew cryptococcus albidus, one grew sporobolomyces, one had large numbers of peptostreptococcus micros and propionibacterium acnes, greater than 10(6) colony-forming units per gram (cfu/gm), ...19863722630
[a case of peptostreptococcal meningitis associated with subarachnoid hemorrhage and subdural hematoma].a very rare case of peptostreptococcal meningitis associated with subarachnoid hemorrhage and subdural hematoma was reported. a 25-year-old man was admitted to st. mary's hospital on november 6, 1984 with a few day's history of headache and low grade fever. on admission, he had high grade fever (39.2 degrees c) and tachycardia (110/min). there were no neurological deficits other than neck stiffness and kernig's sign. the cerebrospinal fluid (csf) which was obtained through lumbar puncture showed ...19863741708
[changes in pathogenic microbes in infectious diseases--infections in otorhinolaryngologic diseases]. 19863761585
[the role of intrauterine contraceptive devices in the development of inflammatory processes in the small pelvis].the incidence of pelvic inflammatory disease (pid) attributable to iud use has been increasing, especially after the removal of the dalkon shield from the market, but this relationship has not been settled conclusively. in recent decades pid included a variety of infections, but lately the definition of pid has meant acute ascending infections of the female genital tract. its most common risk factors include promiscuity of iud use, although this can be reduced to one fourth by regular checkups ...19863763205
[meningitis and brain abscess caused by anaerobic bacteria in an infant]. 19863765691
[effect of metronidazole on the course of experimental anaerobic streptococcal pneumonia].the effect of metronidazole in experimental anaerobic pneumonia in guinea pigs infected with peptostreptococci has been studied. in the untreated animals the prolonged pathological process in the lungs is mainly associated with the suppression of the functional state of the thymus-dependent link of the immune system. metronidazole suppresses anaerobic flora, stimulates local immune reaction and arrests the development of inflammatory changes in the lungs. the drug eliminates the unbalance in som ...19863765970
diabetic foot infections. bacteriologic analysis.diabetic patients with foot infections were prospectively evaluated over a two-year period. cultures from reliable specimens avoiding contamination with foot ulcers were obtained in 54 infectious episodes. staphylococcus species, enterococcus species, corynebacterium species, and various species of enterobacteriaceae were commonly isolated. common anaerobic isolates included peptostreptococcus magnus, peptostreptococcus prevotii, and bacteroides species. results of cultures from 94 unreliable sp ...19863767539
peptostreptococcal vertebral osteomyelitis.the clinical course of a patient with vertebral osteomyelitis caused by peptostreptococcus micros is described. the organism was isolated on two occasions from lumbar vertebral biopsies and was visualized in histologic sections of one of the biopsies. the patient had no apparent infections at other body sites. the infection was eradicated after 6 weeks of intravenous clindamycin therapy.19863771752
[studies of the sensitivity of absolute anaerobes to antibiotics and metronidazole (determination of minimal inhibitory concentrations-mic)]. 19863776192
isolation and characterization of a mucin-degrading strain of peptostreptococcus from rat intestinal tract.a mucin-degrading microorganism was isolated from the intestinal tract by serial sectioning from the serosal side of the caecum wall from a conventional rat. the ability of degrading the intestinal water-soluble mucin was present both in vivo after monocontamination of germ-free rats and in vitro, when adding the microbe to mucin medium. the morphology, gram-positive cocci single or in short chains and the very weak biochemical activities allow us to place this strain in the species peptostrepto ...19863788570
short-term failure of iud removal to alter bacterial flora in a patient with chronic anaerobic endometritis.a patient with cytological and clinical evidence of iud-associated anaerobic endometritis was studied with quantitative and qualitative bacteriological techniques. with the exception of elimination of actinomyces israelii from the deep endocervical/endometrial culture, iud removal induced minimal qualitative changes in the bacterial flora of the endocervical/endometrial sample during the 35 days the patient was monitored. the interposition of menstruation did not significantly alter either the q ...19863789249
mutagenic activation of biliary metabolites of benzo(a)pyrene by beta-glucuronidase-positive bacteria in human faeces.human faeces hydrolysed synthetic beta-d-glucuronides of both p-nitrophenol and phenolphthalein. the origin of this activity in faeces was localised in the bacterial pellet fraction after centrifugation. ninety-seven bacterial strains with beta-glucuronidase activity isolated from fresh human faeces were identified as species of bacteroides, peptostreptococcus, fusobacterium, propionibacterium, clostridium, eubacterium and bifidobacterium. they were classified into two groups according to their ...19863795254
proline requirement for glucose utilization by peptostreptococcus anaerobius atcc 27337.resting cells of peptostreptococcus anaerobius maintained under anaerobic conditions were unable to metabolize either glucose or alanine. the addition of proline to the appropriate suspension, however, resulted in the immediate utilization of both compounds. fermentation of alanine by the cells required that stoichiometric concentrations of proline be present in the medium; and during the oxidation of alanine, proline was simultaneously reduced to the ring cleavage product delta-aminovaleric aci ...19873804441
analysis of edta-soluble cell surface components of gram-positive anaerobic cocci.the protein content of edta extracts from 76 strains of gram-positive anaerobic cocci was examined using sds-page. strains of peptostreptococcus anaerobius produced almost identical profiles; greater heterogeneity was observed within the species peptococcus magnus, peptococcus prevotii and peptococcus asaccharolyticus, but several strains within each biotype produced similar patterns. serological investigation of these extracts by elisa revealed numerous cross-reactions among the different bioty ...19863806050
microbiology of retropharyngeal abscesses in children.aspiration of retropharyngeal abscesses was performed in 14 children. cultures were taken from aspirates for aerobic and anaerobic bacteria, and all yielded bacterial growth. anaerobes were isolated in all patients; they were the only organisms isolated in two patients (14%) and were mixed with aerobes in 12 patients (86%). there were 78 anaerobic isolates (5.6 per specimen). the predominant anaerobes were bacteroides species, peptostreptococcus species, and fusobacterium species. there were 26 ...19873812388
bacteraemia and seeding of capsulate bacteroides spp. and anaerobic cocci.the effect of capsulation on the ability of bacteroides fragilis, b. asaccharolyticus and anaerobic gram-positive cocci to induce bacteraemia and seeding to various organs was investigated. the test species were injected into mice subcutaneously alone, or mixed with other aerobic or facultative organisms. capsulate anaerobes were isolated more frequently from the blood, spleen, liver, and kidneys of infected animals than were non-capsulate organisms. after injection of single anaerobic strains, ...19873820272
[2 cases of pneumonia with empyema caused by anaerobic micro-organisms in childhood]. 19863826924
enzymatic reduction of sennidin and sennoside in peptostreptococcus intermedius.peptostreptococcus intermedius, one of the dominant bacteria of human intestine, reduced sennidin and sennoside to rheinanthrone and 8-glucosyl-rheinanthrone, respectively, and these reduction rates were stimulated by the addition of nicotinamide adenine dinucleotide (reduced form) (nadh), flavin adenine dinucleotide (fad) and glucose. the reduction was accelerated by removing oxygen from the incubation tubes, which indicates the inhibition of the reduction by o2. thus, for the maximal activity, ...19853841560
effect of aspoxicillin on anaerobic bacteria.aspoxicillin (aspc), a semisynthetic penicillin has a broad spectrum of antibacterial activities against gram-positive and gram-negative anaerobic bacteria. its in vitro antibacterial activity was less than those of cefoxitin against peptostreptococcus and veillonella, but was significantly high against bacteroides fragilis, one of the most clinically important anaerobe. the therapeutic and/or protective effect of aspc in experimental subcutaneous abscess or experimental intraabdominal mixed inf ...19853850132
survival analysis of periodontal sites before and after periodontal therapy.periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ...19853860518
predominant obligate anaerobes in human carious dentin.with the adoption of an anaerobic glove box system, we isolated and identified the predominant micro-organisms in lesions of carious dentin from four permanent third molars. the overwhelming majority of the micro-organisms isolated from both shallow and deep layers of carious dentin were obligate anaerobes, suggesting that conditions in the dentin were strictly anaerobic. the predominant obligate anaerobes were gram-positive rods which were identified as members of the following genera: propioni ...19853861648
anaerobic infections. part i. 19853862516
antibiotic susceptibilities of periodontal bacteria. in vitro susceptibilities to eight antimicrobial vitro susceptibilities of 369 to 966 bacterial isolates from periodontal lesions to eight antibiotics were determined by agar dilution technique as a means of determining which antimicrobial agents were inhibitory for bacteria frequently associated with destructive periodontal diseases. although most bacteria were relatively susceptible to the penicillins, greater activity was generally noted with amoxicillin than with either penicillin or ampicillin with the exception of selenomonas sputigen ...19853866054
purification and characterization of hyaluronidase from oral peptostreptococcus species.hyaluronidase was purified to apparent homogeneity from the spent medium of peptostreptococcus sp. strain 84h14s. the enzyme was purified 310-fold by ethanol precipitation, gel chromatography, and cation-exchange chromatography with a recovery of 42% of the original activity in the culture medium. the molecular weight of the purified enzyme was estimated to be 160,000 by gel filtration with sephacryl s-300. like bacterial mucopolysaccharidases of other sources, the enzyme carried out an eliminat ...19853881352
anaerobic infections of burns.from august 1980 to june 1982, 102 burn wound specimens taken from 34 patients were studied for anaerobic cultures. fifteen instances (14.7 per cent) from 8 patients were positive and altogether 12 species were found. the predominant anaerobes were bacteroides melaninogenicus, peptococcus, bacteroides fragilis, and other strains of bacteroides and peptostreptococcus. they were mostly discovered in electric burn wounds and burn wounds affecting the perianal and oral areas. wounds with anaerobic i ...19853886089
a microaerophilic coccus in pyogenic infections of ruminants.pyogenic infections of cattle, sheep and goats were examined for the presence of a gram positive bacterium that has been designated "microaerophilic coccus" by other workers. the bacterium was found to be involved in a range of disease processes, including foot and soft tissue abscesses, mastitis, pericarditis and pyometra in cattle, joint and foot abscesses in sheep and foot abscesses in goats. the characteristic feature of the bacterium was its satellitic growth around colonies of other organi ...19853888166
peritonsillar abscess: bacteriological evaluation.the pus from a series of 41 peritonsillar abscesses was examined bacteriologically. in the majority of the abscesses a mixed bacterial flora was found. the specimens yielded 0-7 different bacterial species per abscess (mean 3.0). one species alone was isolated only in five cases (12.5%). both anaerobic and aerobic bacteria were isolated from the specimens of 25 patients (61%), only anaerobes from two specimens (4.9%), and only facultative bacteria from 12 specimens (29%). beta haemolytic strepto ...19853891156
comparison of the pras ii, an-ident, and rapid-ana systems for identification of anaerobic bacteria.two rapid systems for the identification of anaerobes were compared to a conventional growth system aided by a computer. the rapid systems (an-ident and rapid-ana) are non-growth-dependent micromethods that identify anaerobes in 4 h by the action of various constitutive enzymes on chromogenic substrates. the organisms tested were 98 anaerobes, most of which were clinical isolates. the an-ident system identified 76 of these to species level and 86 to genus level; the rapid-ana system correctly id ...19853894412
moxalactam versus clindamycin plus tobramycin in the treatment of obstetric and gynecologic infections.the clinical efficacy of moxalactam versus clindamycin/tobramycin was evaluated in a comparative, randomized, prospective study. sixty patients were treated: 30 with moxalactam and 30 with clindamycin/tobramycin. there were 15 cases of tuboovarian abscess, 36 cases of severe pelvic inflammatory disease with peritonitis, eight cases of endomyometritis, and one wound abscess. aerobic and anaerobic cultures from the sites of infection yielded 441 microorganisms from 53 patients; an average of 8.3 b ...19853895947
anaerobic peptostreptococcal keratitis. 19853895968
comparative clinical evaluation of imipenem/cilastatin vs. cefotaxime in treatment of severe bacterial patients with severe bacterial infections caused by organisms susceptible to imipenem and cefotaxime were given either imipenem/cilastatin sodium (mk0787/mk0791) or cefotaxime as a part of a multiclinic randomized study to evaluate the effectiveness, safety, and tolerability of imipenem/cilastatin. clinical diagnoses included bacteremia, urinary tract infection, osteomyelitis, mediastinitis, lower respiratory tract infection, and soft tissue infection. efficacy was evaluated for 10 patient ...19853901208
imipenem/cilastatin in the treatment of obstetric and gynecologic infections: a review of worldwide experience.worldwide experience with imipenem/cilastatin in the treatment of 72 patients with a variety of obstetric and gynecologic infections is reviewed. clinical cure or improvement occurred in 97% of 72 assessable patients. the most common etiologic pathogens were escherichia coli, group b streptococci, neisseria gonorrhoeae, staphylococcus epidermidis, enterococci, bacteroides bivius, bacteroides species, bacteroides fragilis, and peptostreptococcus. of the recovered pathogens, 99.5% were susceptible ...19853901216
evaluation of the rapid-ana system for identification of anaerobic bacteria of veterinary origin.this study evaluated the ability of the rapid-ana system (innovative diagnostic systems, inc., atlanta, ga.) to accurately identify a spectrum of freshly isolated veterinary anaerobes. a total of 183 isolates were tested and included 7 actinomyces spp., 53 bacteroides spp., 32 clostridium spp., 2 eubacterium spp., 65 fusobacterium spp., 1 peptococcus spp., 22 peptostreptococcus spp., and 1 propionibacterium spp. all isolates were initially identified by conventional biochemical testing and gas-l ...19853905854
the classification and identification of the anaerobic gram-positive cocci.the approved lists of bacterial names (1980) includes the following peptococcus and peptostreptococcus species pathogenic for man: peptococcus magnus, p. prevotii, p. asaccharolyticus, peptostreptococcus anaerobius, p. micros and p. productus. a presumptive identification of these species can be made on the basis of cellular morphology, susceptibility to novobiocin, characterization of metabolic end products, fermentation of glucose and lactose, and production of indol. capnophilic and microaero ...19853906854
respiratory infections: clinical evaluation.a review of clinical studies of piperacillin shows that it is valuable for the treatment of respiratory infections due to enterobacteriaceae, pseudomonas sp, anaerobes, and mixed flora including anaerobes. various studies of a total of 420 patients treated with piperacillin for lower respiratory tract infections found that 97% of the patients were cured or markedly improved. piperacillin has also been found as effective as combination therapy (gentamicin or tobramycin plus carbenicillin or ticar ...19853907845
in vitro activity of ceftazidime in combination with other antibiotics.189 bacterial strains were investigated for their in vitro sensitivity against ceftazidime (alone and in combination with another antibiotic). moreover, the possibility to prevent development of secondary bacterial resistance as observed in subcultures at subinhibitory antibiotic concentrations, was studied using specific antibiotic combinations. of 115 staphylococcal strains (91 strains of staphylococcus aureus, 24 strains of staphylococcus epidermidis), 2% were sensitive, 82% were moderately s ...19853930407
[bacteriological study of cefminox in the field of obstetrics and gynecology].cefminox (cmnx, mt-141), a new cephem antibiotic, was determined of its antibacterial activity against 304 clinical isolates with following results. cmnx was inferior to cez or cmz in the activity against 78 isolates of staphylococcus sp., but it was superior to these antibiotics in the activity against 104 isolates of e. coli. against 53 isolates of bacteroides sp., cmnx showed higher activity than cez or cmz. in the activity against 69 isolates of peptococcus sp. and peptostreptococcus sp., cm ...19853930791
[studies of isolated organisms from otorhinolaryngological infections and their susceptibilities to macrolide antibiotics].to study current situation of pathogenic bacteria and their drug resistance to macrolide antibiotics in the otorhinolaryngological infections, 609 strains diagnosed as pathogen derived from 463 patients were collected from cohospitals or institutions during the period of 1980-1983. the results obtained were as follows: gram-positive cocci (gpc) was dominant (410 strains) and major species were s. aureus (135 strains), s. pneumoniae (81 strains), s. epidermidis (68 strains) and s. pyogenes (65 st ...19853930804
arthropathic properties of cell wall polymers from normal flora bacteria.peptidoglycan-polysaccharide (pg-ps) fragments were purified from cell walls of group d streptococci (streptococcus faecium, strains atcc 9790 and f-24) with a protocol which minimizes autolytic activity and tested for ability to induce arthritis in rats. pg-ps fragments from cell walls of other normal flora bacteria (peptostreptococcus productus, and propionibacterium acnes), group a streptococci, and pseudomurein-ps fragments from cell walls of methanobacterium formicicum, were similarly purif ...19863940996
survival of gram positive anaerobic cocci on swabs and their isolation from the mouth and vagina.the survival of gram positive anaerobic cocci on plain cotton wool and albumin coated swabs held in various transport media was investigated. results suggested that in most cases amies', stuart's and vmgii media do not offer any more protection to the bacteria than storing swabs dry in their containers. a technique was developed for the isolation and identification of gram positive anaerobic cocci from the mouth and vagina, incorporating bicozamycin in the medium as a selective agent. few strain ...19863950035
peptococcaceae bacteremia.bacteremia with peptococcaceae is an uncommon clinical manifestation of infection with this family of microorganisms. a 20-month review of 12 patients with bacteremic infections due to anaerobic gram-positive cocci revealed that obstetrical patients during the peripartum period constitute the group at greatest risk for the development of such infections. eight of the 12 patients were young women hospitalized on the obstetrical service. seven patients had postpartum endometritis and one patient h ...19863956135
[hyperbaric oxygen therapy of anaerobic brain abscesses following tonsillectomy].this study describes the clinical course of a 31 year old woman who developed multiple anaerobic brain abscesses six days after tonsillectomy, followed by hemoparesis and dysarthria. in spite of craniotomy, repeated punctures and drainage of pus and high dose local and systemic antibiotics, there was an obvious deterioration in the patient's condition. hyperbaric oxygen therapy was tried as a last resort. the patient improved quickly, and six months after the tonsillectomy seems to be neurologic ...19853980250
mutagenic activation of biliary metabolites of 1-nitropyrene by intestinal investigate the modifying role of intestinal microflora in the metabolism of chemical carcinogens in vivo, we subjected bile from fischer rats treated per os with chemical carcinogens and related compounds to a mutagenicity assay in the presence and absence of a cell-free extract from human feces. a mixture of the bile sample and potassium phosphate buffer was incubated in the presence or absence of human cell-free fecal extract and then further incubated with a bacterial suspension of salmon ...19853982438
serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe the present study we sought to determine whether serum antibody was present against microorganisms which predominate in the subgingival flora of young adults with generalized severe periodontitis (sp). subjects with sp were often seropositive for eubacterium brachy, fusobacterium nucleatum e3c22, and peptostreptococcus micros, whereas subjects with juvenile periodontitis (jp) and subjects with healthy periodontium (hp) were not. both sp and jp subjects were more frequently seropositive for ba ...19853988337
antimicrobial susceptibilities of anaerobic bacteria isolated from female genital tract infections.certain species or subspecies of anaerobic bacteria are isolated with higher frequency from female genital tract infections than from other anatomic sites. to gain susceptibility data more specific to the treatment of these infections, nine antimicrobial agents were tested by an agar dilution technique against 230 anaerobic bacteria isolated solely from obstetric and gynecological infections. these genital isolates were, in general, very susceptible to imipenem (most active, inhibiting all gram- ...19853994347
beta-glucuronidase activities of intestinal bacteria determined both in vitro and in vivo in gnotobiotic rats.the beta-glucuronidase activities of bacterial strains isolated from the rat intestinal tract were studied both in vitro in culture media and in vivo in the intestinal contents of gnotobiotic rats. only 50 of 407 strains tested were found to be positive in vitro. they belonged to the three genera clostridium, peptostreptococcus, and staphylococcus. the in vitro-negative strains were also negative in vivo. the beta-glucuronidase activities of the beta-glucuronidase activities of the positive stra ...19853994372
changes in prevalence and susceptibility of obligate anaerobes in clinical veterinary practice.of 3,133 clinical specimens obtained from domestic animals, 26% contained species of bacteria that were obligate anaerobes. members of the genera bacteroides, fusobacterium, and peptostreptococcus accounted for 77% of the isolates. on the average, 2 species of obligate anaerobes were found in each specimen, usually admixed with facultatively anaerobic bacteria. of those specimens containing an obligate anaerobe, 20% contained one isolate that was resistant to penicillin, ampicillin, and cephalot ...19853997623
anaerobic bacteria in urine before and after prostatic massage of infertile the present study 25 infertile men delivered urine before and after prostatic massage. expressed prostatic secretion (eps) was obtained from 11 men. aerobic and anaerobic bacterial analyses showed that a total of 33 isolates was found in samples of urine voided before massage as compared to 59 isolates after massage of the prostate. there was an increase in the number of anaerobes whereas there was no change in the number of aerobes. the occurrence of eps did not influence the number of aerob ...19854000063
in vitro activity of cefbuperazone, a new cephamycin, against anaerobic bacteria.the 90% mic of cefbuperazone (bmy 25182) was 32 micrograms/ml for bacteroides fragilis and bacteroides spp., 128 micrograms/ml for fusobacterium and clostridium spp., 64 micrograms/ml for eubacterium and peptococcus spp., 8 micrograms/ml for actinomyces spp., and 32 micrograms/ml for peptostreptococcus spp. the level of activity of cefbuperazone was higher against b. fragilis and lower against anaerobic cocci than those of related cephalosporins, i.e., cefoxitin, cefoperazone, cefotaxime, ceftiz ...19854004195
[a case of preputial calculi].a case of a 73-year-old patient with preputial calculi is presented. the patient visited our hospital complaining of consciousness loss. it was impossible to place a catheter because of complete phimosis. palpatory examination suggested the presence of stones in preputial cavity. dorsal incision was made. after pus discharge nine small stones were extirpated. ammonium hydrogen urate (96%) and calcium phosphate (4%) were found in these stones with infrared spectroscopic analysis. bacteriodes ureo ...19854013948
change in the volatile fatty acids content of laboratory stored sterilized and non-sterilized swine wastes.the influence of urine and microbial activity was studied as possible factors leading to the generation of volatile fatty acids in non-sterilized stored swine wastes. urine was found to promote the generation of the volatile acids. the microbial origin of the volatile acids in the wastes was ascertained by the growth of an anaerobic bacterial strain (peptostreptococcus anaerobius). significant generation and transformation of these acids was observed in sterilized swine waste samples stored unde ...19854033458
a sodium ion gradient as energy source for peptostreptococcus asaccharolyticus.the determination of enzymatic activities in cell-free extracts of acidaminococcus fermentans and peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (r)-2-hydroxyglutarate to acetate and butyrate. from the ratio of these products the amount of atp generated by substrate level phosphorylation was calculated. growth experiments with the organisms including clostridium symbiosum and clostridium tetanomorphum indicated that a sodium gradient con ...19854037980
[non-puerperal mastitis. etiology, clinical aspects and therapy].non-puerperal mastitis was diagnosed in 79 patients (aged 12-77 years) over the years 1974-1984. malignant neoplasm was not present. bacterial infection in the region of the areola was the most frequent finding (40%), followed by abacterial inflammation without involvement of the nipples (29%). the other cases, bacterial or nonbacterial, occurred at different sites. the histological picture or clinical features of an increased secretory activity of the mammary gland (galactorrhoea, mastodynia) i ...19854038482
rapidly cavitating nodules in a young man. 19854042712
analysis of gram-positive anaerobic cocci in oral, fecal and vaginal flora. 19854043069
qualitative and quantitative changes of the vaginal microbial flora during the menstrual cycle.knowledge of the normal vaginal microbial flora is essential in understanding the etiology of female genital tract infections. this study was done to investigate both qualitative and quantitative differences that occur during the menstrual cycle. we studied 34 reproductive age women longitudinally. quantitative cultures were obtained during menses and following the cessation of menses. a greater variety of organisms were found during menstruation. the effect was not selective with respect to aer ...19854051082
[comparative study in vitro on the activity of 7 antibiotics against anaerobic bacteria from hospital isolates].one hundred and ninety-four anaerobic bacteria isolated from clinical sources were tested by agar dilution technique against seven antimicrobial agents. a comparison with international literature, generally used for selecting appropriate antimicrobial drug showed concordant patterns of susceptibility in the case of cocci gram-positive, clostridia, fusobacterium and other species of bacteroides excluded b. fragilis group. for these strains we did not found any particular problem in the choice of ...19854074510
[etiology of bacterial vaginosis (non-specific vaginitis)].56 women who were diagnosed bioclinically as having a bacterial vaginal infection were studied, as were 35 women as a control group. the study was a semi-quantitative analysis of the vaginal bacterial flora, both aerobic and anaerobic. it shows that gardnerella vaginalis and anaerobic bacteria such as peptococcus, peptostreptococcus, bacteroïdes, veillonella and mobiluncus were associated in a statistically significant way with bacterial vaginitis. on the other hand lactobacilli were less freque ...19854078248
[fundamental and clinical studies of cefpiramide in the field of obstetrics and gynecology].fundamental and clinical studies of cefpiramide (cpm, sm-1652) a new semisynthetic cephalosporin were carried out in the field of obstetrics and gynecology. the results were obtained as follows: in vitro antibacterial activity of cpm against recent 255 clinical isolates was compared with those of cefazolin (cez), cefmetazole (cmz) and cefoperazone (cpz). cpm showed strong antibacterial activity against staphylococcus, k. pneumoniae, peptococcus and peptostreptococcus. however the minimum inhibit ...19854079009
[fundamental and clinical studies of cefpiramide in the field of obstetrics and gynecology].cefpiramide (cpm, sm-1652), a new cephem antibiotic, was fundamentally and clinically studied. the following results were obtained. serum and internal genital tissue levels of cpm were measured following intravenous drip infusion of 1 g. high serum levels of 30 micrograms/ml and tissue levels of more than 4 micrograms/g were at least maintained for 8 hours. favourable transfer of cpm into the pelvic dead space exudate was observed. the exudate level was 7.25 micrograms/ml on average even at 8 ho ...19854079011
whipple disease: light and electron microscopy study. 19744138040
fluorescent antibody study of the gram-positive anaerobic cocci.fluorescent antibody conjugates were prepared from five species of anaerobic cocci commonly isolated from human infections. when tested with homologous and heterologous cells these conjugates were found to be highly specific. there was no evidence of a common genus antigen. peptococcus magnus conjugates detected a species-specific antigen; cross-reactions with peptostreptococcus anaerobius were readily eliminated by absorption. the conjugates from peptococcus asaccharolyticus, peptococcus prevot ...19744140708
[differentiation between anaerobic organisms using gaschromatographic metabolic analyses]. 19724145622
[the fecal flora in man. i. composition of the fecal flora of various age groups]. 19734145840
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