PMID(sorted descending)
infected neonatal cephalohematomas caused by anaerobic present the microbiological and clinical features of six children with infected cephalohematomas (ic) caused by anaerobic bacteria.200515914351
associations between igg antibody to oral organisms and carotid intima-medial thickness in community-dwelling adults.the aims of this study are to describe the relationships between igg antibodies to 17 oral organisms and atherosclerosis as indexed by carotid intima-medial wall thickness (imt) and to evaluate the role of smoking.200515893320
bacteriology of chronic maxillary sinusitis associated with nasal polyposis.aspirates from 48 chronically inflamed maxillary sinuses from patients who had nasal polyposis were processed for aerobic and anaerobic bacteria. bacterial growth was present in 46 (96%) specimens. aerobic or facultative bacteria were present in 6 (13%) specimens, anaerobic bacteria alone in 18 (39%), and mixed aerobic and anaerobic bacteria in 22 (48%). there were 110 bacterial isolates (2.4 per specimen). thirty-nine of the isolates were aerobic or facultative organisms (0.85 per specimen). th ...200515888469
tobacco smoking and periodontal microflora in a saudi arabian explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits.200515882210
microbiology of acute and chronic maxillary sinusitis associated with an odontogenic origin.: to study the microbiology of sinusitis associated with odontogenic origin.200515867647
mechanically unfolding the small, topologically simple protein l.beta-sheet proteins are generally more able to resist mechanical deformation than alpha-helical proteins. experiments measuring the mechanical resistance of beta-sheet proteins extended by their termini led to the hypothesis that parallel, directly hydrogen-bonded terminal beta-strands provide the greatest mechanical strength. here we test this hypothesis by measuring the mechanical properties of protein l, a domain with a topology predicted to be mechanically strong, but with no known mechanica ...200515863479
chicken anti-protein l for the detection of small amounts of protein l in the presence of igg.protein l is a cell surface protein, expressed by peptostreptoccocus magnus, which binds to the variable light chains of immunoglobulins without interfering with antigen binding. it can be used for purification of mammalian antibodies of all classes in contrast to the ig-binding proteins protein a and protein g. detection of protein l leakage into antibody preparations is important, since protein l could interfere in immunological assays and cause adverse reactions in vivo. here we have develope ...200515857176
detection of bacteria in platelet concentrates: comparison of broad-range real-time 16s rdna polymerase chain reaction and automated culturing.based on real-time polymerase chain reaction (pcr) technology, a broad-range 16s rdna assay was validated and its performance was compared to that of an automated culture system to determine its usefulness for rapid routine screening of platelet concentrates (pcs).200515847662
protein l-agarose for adsorption of autoantibodies: a potential tool for extracorporeal treatment.this work investigated the adsorption of autoantibodies such as anti-ss-a/ro, anti-ss-b/la, anti-sm, and anti-dsdna on protein l-agarose gel. in order to determine better conditions for igg adsorption on this matrix, some buffer systems were tested. adsorption data were analyzed using the langmuir and langmuir-freundlich isotherm models. the experimental isotherms were best described by the langmuir-freundlich model, which indicated negative and positive cooperativities for binding in the presen ...200515787626
influence of denatured and intermediate states of folding on protein aggregation.we simulate the aggregation thermodynamics and kinetics of proteins l and g, each of which self-assembles to the same alpha/beta [corrected] topology through distinct folding mechanisms. we find that the aggregation kinetics of both proteins at an experimentally relevant concentration exhibit both fast and slow aggregation pathways, although a greater proportion of protein g aggregation events are slow relative to those of found for protein l. these kinetic differences are correlated with the am ...200515772307
anaerobic spondylodiscitis: case series and systematic review.bacterial spondylodiscitis is rarely caused by anaerobic organisms. we describe two patients with lumbar vertebral osteomyelitis and discitis caused by anaerobic bacteria, including an unusual occurrence after an endodontic procedure, and review the salient clinical features and outcomes of 31 previously reported cases.200515759942
[pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology].this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria.200515757581
microbiology and management of infected neck review and describe the microbiology, diagnosis, and management of infected neck cysts.200515742293
engineering high affinity superantigens by phage display.protein l (ppl) is a b-cell superantigen from peptostreptococcus magnus known to bind to mammalian vkappa light chains. ppl from p.magnus strain 312 comprises five homologous immunoglobulin (ig) binding domains. we first analysed the binding of the individual domains (b1-b5) of ppl(312) to human vkappa light chains (huvkappa) subtypes 1 (huvkappai) and 3 (huvkappaiii). using a combination of rational design and phage selection we isolated mutants of the n-terminal b1 domain with a 14-fold increa ...200515733921
[frequency, diagnostic criteria and consequences of bacterial vaginosis in pregnant women].vaginal discharge based on changes in a vaginal ecosystem (decreasing number of lactobacillus and increasing number of mobiluncus spp., gardnerella vaginalis, prevotella spp., mycoplasma hominis, peptostreptococcus spp) appears to be a major cause of bacterial vaginosis. bacterial vaginosis is associated with: preterm delivery, premature rupture of membranes, chorioamnionitis, amniotic fluid infection, endometritis, low-weight neonates. the role of natural microflora of vagina, the most importan ...200415730019
properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an escherichia coli host.peptostreptococcus asaccharolyticus glutamate dehydrogenase (l-glutamate: nad+ oxidoreductase (deaminating); ec overexpressed in escherichia coli has been purified by two new methods. enzyme made by the first method showed remarkable thermophilicity, with a temperature optimum of 60 degrees c, and also thermostability, which suggested the second, simpler method, incorporating a heat step. this produced 94 mg of homogeneous protein per litre culture medium. the basic kinetic parameters f ...200515727821
binding of actinobacillus actinomycetemcomitans lipopolysaccharides to peptostreptococcus micros stimulates tumor necrosis factor alpha production by macrophage-like cells.peptostreptococcus micros is a gram-positive bacterium that has been associated with periodontitis and endodontic infections. in this study, we hypothesized that p. micros binds the immunomodulating component lipopolysaccharide derived from gram-negative bacteria to increase its capacity to stimulate cytokine production by host cells. the ability of p. micros to bind actinobacillus actinomycetemcomitans lipopolysaccharide was demonstrated by an enzyme-linked immunosorbent assay and by immunoelec ...200515720573
micromonas (peptostreptococcus) micros: unusual case of prosthetic joint infection associated with dental procedures.micromonas (peptostreptococcus) micros is frequently associated with periodontal disease as well as respiratory, gastrointestinal and female genitourinary tract infections, but only rarely has been reported as a pathogenic agent of prosthetic joint infections. here we describe a case of a 63-year-old woman with prosthetic joint infection of total hip arthroplasty caused by the anaerobic species micromonas micros, associated with tooth extraction. samples obtained intraoperatively and from the or ...200515715175
influence of antimicrobial treatment for helicobacter pylori infection on the intestinal microflora in japanese macaques.eradication treatment for helicobacter pylori is known to cause mild but relatively frequent adverse effects. some adverse effects such as diarrhoea and soft stools are related to disruption of the composition of the intestinal microflora. this study investigated the microfloral changes resulting from administration of an eradication regimen using proton pump inhibitor (ppi), amoxycillin and clarithromycin. twenty-eight laboratory-bred japanese macaques either were administered eradication treat ...200515713617
pharmacokinetics and pharmacodynamics of linezolid in obese patients with cellulitis.linezolid is an oxazolidinone antimicrobial with excellent oral bioavailability and tissue penetration and is active against multidrug-resistant skin/soft tissue pathogens.200515701775
recovery of anaerobic bacteria from wounds after lawn-mower injuries.accidental injury while using lawn mowers can cause serious infectious complications in the injured extremity. anaerobic bacteria were rarely recovered from this infection. two children who sustained injury in their foot by a lawn mower developed severe wound infection. culture of the wound from 1 patient had heavy growth of clostridium bifermentans and peptostreptococcus magnus, and the culture from the other child grew clostridium perfringens. antimicrobial therapy directed at the pathogens an ...200515699821
traumatic cholesteatoma presenting as a brain abscess: a case discuss the clinical aspects and management of traumatic cholesteatomas of the temporal bone.200515699721
periodontal infections and pre-term low birth weight: a case-control study.pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ...200515691348
synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein l from peptostreptococcus magnus.rational design and combinatorial chemistry were utilized to search for lead protein l (ppl) mimetics for application as affinity ligands for the purification of antibodies and small fragments, such as fab and scfv, and as potential diagnostic or therapeutic agents. inspection of the key structural features of the complex between ppl and human fab prompted the de novo design and combinatorial synthesis of a 169-membered solid-phase ligand library, which was assessed for binding to human igg and ...200515688433
alteration in the endogenous intestinal flora of swiss webster mice by experimental angiostrongylus costaricensis infection.the association between worm infections and bacterial diseases has only recently been emphasized. this study examined the effect of experimental angiostrongylus costaricensis infection on endogenous intestinal flora of swiss webster mice. eight mice aging six weeks were selected for this experiment. four were infected with a. costaricensis and the other four were used as controls. twenty eight days after the worm infection, all mice in both groups were sacrificed and samples of the contents of t ...200415654427
use of bioinformatics and pcr in the search for abc transporter homology among various bacteria.bioinformatics databases and search tools are utilised to produce polymerase chain reaction (pcr) primers for the amplification of an abc transporter gene from the clinically important anaerobe finegoldia magna. on sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of enterococcus faecalis. little sequence data is available for f. magna and the newly isolated dna could be a useful tool in the identification of this organism in clinical specimens.200415649009
b cell superantigens: a microbe's answer to innate-like b cells and natural antibodies.marginal zone b cells and b-1 cells have been termed innate-like b cells as they express limited repertoires that play special roles in immune defenses against common infections. these b cells are the sources of natural antibodies and are capable of highly accelerated clonal responses that help counter blood-borne infections. we have characterized a class of microbial product with highly adapted binding interactions with host immunoglobulins/b cell receptors (bcrs), which enable the targeting of ...200515633015
estimates of methyl 13c and 1h csa values (deltasigma) in proteins from cross-correlated spin relaxation.simple pulse schemes are presented for the measurement of methyl (13)c and (1)h csa values from (1)h-(13)c dipole/(13)c csa and (1)h-(13)c dipole/(1)h csa cross-correlated relaxation. the methodology is applied to protein l and malate synthase g. average (13)c csa values are considerably smaller for ile than leu/val (17 vs 25 ppm) and are in good agreement with previous solid state nmr studies of powders of amino acids and dipeptides and in reasonable agreement with quantum-chemical dft calculat ...200415630560
[clinical and bacteriological substantiation of the use of cefoperazone/sulbactam in complex therapy of patients with pyo-destructive forms of lower respiratory tract infection (lrti)].antibacterial therapy is the most important component of the complex management of pyo-destructive forms of lrti. since the microbial flora is rather variable and polymorphous, antibiotics used in the treatment of lrti should be active against both aerobic (especially gramnegative) and anaerobic pathogens. the aim of the study was to prove, on the basis of the bacteriological and clinical findings, the validity of the use of cefoperazone/sulbactam (cs), the only inhibitor-protected cephalosporin ...200415628799
[treatment of acute pyoinflammatory diseases of the lungs and pleura under control of microorganism persistence factors].results of treatment of patients with acute pulmonary abscess, acute empyema of the pleura and pyopneumothorax were analyzed. the local treatment of the main group of patients consisting of 50 persons included oxitocin in combination with antiseptics and antibiotics which facilitated maximal reduction of the persistent potential of the pathogenic agent, its rapid elimination from the purulent focus and allowed to get better results using conservative therapy. the obtained clinical effect confirm ...200415626066
anaerobic pulmonary infections in children.pulmonary infections due to anaerobic bacteria usually occur in children prone to aspiration. the source of the anaerobic bacteria is the oropharyngeal bacterial flora, where these organisms outnumber aerobic and facultative organisms in a 10:1 ratio. the most common lower respiratory tract infections where anaerobic bacteria are recovered mixed with aerobic organisms are aspiration pneumonia, lung abscess, and empyema. the predominant isolated anaerobic bacteria are peptostreptococcus, fusobact ...200415599270
coaggregation profiles of the microflora from root surface caries lesions.bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. as no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single candida spp. the quantitative coa ...200515598414
clinical and microbiological associations in chronic periodontitis.chronic periodontitis is the most prevalent form of periodontal disease. the subgingival microflora has been associated with the onset and progression of the disease. the aim of this work was to establish associations and correlations between clinical indicators and subgingival plaque microbiological data in patients diagnosed with moderate or severe periodontitis. one hundred sites with a probing depth of > or = 5 mm were selected. plaque index (pi), gingival index (gi), bleeding on probing (bp ...200415584257
socket infection due to retained gauze after evisceration.a 64-year-old man had late-onset socket infection due to retained surgical gauze after evisceration. external examination showed a mass of retained surgical gauze with copious yellowish discharge and gas bubbles. computed tomography scans showed a 3.2 x 2.4 x 2.4-cm heterogeneous mass and numerous gas bubbles. culture of the discharge yielded pseudomonas aeruginosa, streptococcus viridans, peptostreptococcus species, and fusobacterium species. surgical debridement and antibiotic therapy achieved ...200415580975
periimplantitis.orodental rehabilitation through the use of implants offers very high success rates. in this paper, we describe some of the complications involved with this technique, such as periimplant disease and, within this category, periimplantitis, an inflammatory reaction in which there is a loss of the bony support of the implant accompanied by inflammation. the aetiology of the disease is conditioned by the status of the tissue surrounding the implant, implant design, degree of roughness, the poor ali ...200415580138
microbiological basis of oral infections and sensitivity to antibiotics.because oral infections are common, the physician must understand the underlying etiology, pathogeny, and other variables that determine how these processes evolve in order to choose the most appropriate antibiotic drug. the special characteristics of the oral cavity determine the make-up of the microflora that lives there. different anaerobic species belonging to the peptostreptococcus, prevotella, fusobacterium, gemella, and porphyromonas genera are of particular interest, as are the aerobic s ...200415580129
[a case of retroperitoneal abscess by anaerobic bacteria].a case of retroperitoneal abscess with an unknown origin is presented. a 60-year-old woman visited our hospital complaining of fever and right flank pain. abdominal computed tomography and ultrasound examination revealed retroperitoneal abscess posterior to right kidney. percutaneous drainage and administration of antibiotics were performed. then, both symptoms were improved. the fluid culture proved to be anaerobic bacteria, peptostreptococcus species and porphyromonas asaccharolytica. she had ...200415575225
microbiology and management of peritonsillar, retropharyngeal, and parapharyngeal abscesses.this review describes the microbiology, diagnosis, and management of peritonsillar, retropharyngeal, and parapharyngeal abscesses in children. predominant anaerobic organisms isolated in peritonsillar, lateral pharyngeal, and retropharyngeal abscesses are prevotella, porphyromonas, fusobacterium and peptostreptococcus spp.; aerobic organisms are group a streptococcus ( streptococcus pyogenes ), staphylococcus aureus and haemophilus influenzae . anaerobic bacteria can be isolated from most absces ...200415573356
pharmacokinetics and pharmacodynamics of intravenous levofloxacin at 750 milligrams and various doses of metronidazole in healthy adult subjects.the purpose of this investigation was to evaluate the steady-state pharmacokinetics, pharmacodynamics, and safety of intravenous levofloxacin at 750 mg administered once daily combined with three different dosages of intravenous metronidazole (500 mg every 8 h [q8h], 1,000 mg q24h, and 1,500 mg q24h). eighteen healthy adult subjects received all three combinations in a randomized, crossover fashion. serial blood and urine samples were collected on the third day of each study period. the 24-h are ...200415561831
role of anaerobic bacteria in infections following tracheostomy, intubation, or the use of ventilatory tubes in children.colonization of the tracheobronchial tree with microorganisms almost always follows tracheal intubation, tracheostomy, or the use of ventilatory tubes. infection of the tracheostomy wound site frequently occurs after prolonged use of the tracheostomy. the long-term-ventilated child is at high risk for developing tracheobronchitis or nosocomial pneumonia, generally involving aerobic gram-negative or gram-positive bacteria. several studies have illustrated the role of anaerobic bacteria in lower r ...200415535147
rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis.although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ...200415508748
retrospective case review of capsular contracture after two-stage breast reconstruction: is colonization of the tissue expander pocket associated with subsequent implant capsular contracture?periprosthetic capsular contracture is a common problem associated with implant-based breast reconstruction. the purpose of this study was to determine if bacterial colonization of the tissue expander contributes to contracture of the permanent implant. medical records were reviewed for 86 patients (124 tissue expanders) between 1997 and 2001 in 1 institution. three specimens taken from the expander were cultured. the overall incidence of colonization was 42.7%; 49.4% (38.8-60.0) of immediate an ...200415502455
subgingival microbiota of chronic periodontitis subjects from different geographic locations.most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. the purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries.200415491316
the effect of periodontal treatment on the salivary bacterial load and early plaque formation.the purpose of the present study was to investigate in periodontitis patients the relationship between the number of bacteria in the saliva and the amount of de novo plaque formation before and after treatment.200415491312
the role of sequence and structure in protein folding kinetics; the diffusion-collision model applied to proteins l and g.the diffusion-collision model (dcm) is applied to the folding kinetics of protein l and protein g. in the dcm, the two proteins are treated as consisting of two beta-hairpins and one alpha-helix, so that they are isomorphous with the three-helix bundle dcm model. in the absence of sequence dependent factors, both proteins would fold in the same way in the dcm, with the coalescence of the n-terminal hairpin and the helix slightly favored over the c-terminal hairpin and the helix because the forme ...200415458632
variation in antimicrobial action of proanthocyanidins from dorycnium rectum against rumen bacteria.the proanthocyanidin polymer fractions of the leaves of the forage legume dorycnium rectum were analysed by acid catalysis with benzyl mercaptan, nmr and es-ms. the results showed that d. rectum differs from other temperate proanthocyanidin-containing forage legumes in that the range of polymers extends up to very high degrees of polymerisation. three fractions were characterised as low, medium, and high molecular weight proanthocyanidin fractions with mean degree of polymerisations of 10.3, 41 ...200415381413
small intestinal submucosa tension-free sling: postoperative inflammatory reactions and additional data. 200415371839
clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis.the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy.200415367191
surgical infections: a microbiological study.surgical infections are mostly polymicrobial, involving both aerobes and anaerobes. one hundred seventeen cases comprised of abscesses (n=51), secondary peritonitis (n=25), necrotizing fascitis (n=22) and wounds with devitalized tissues (n=19) were studied. the number of microorganisms isolated per lesion was highest in secondary peritonitis (2.32). the aerobe/ anaerobe ratio was 0.81 in secondary peritonitis and 1.8 in necrotizing fascitis. most secondary peritonitis (80%), necrotizing fascitis ...200415361989
[biological properties of microorganisms as the basis for the prognosis of the severity of pyoinflammatory pleuropulmonary diseases].in the study of 65 microbial cultures isolated from the purulent foci in acute pulmonary abscess and acute pyothorax of 48 patients, a wide spectrum of microflora was detected. staphylococci and pseudomonas prevailed among aerobes, bacteroids and peptostreptococci--among anaerobes. in cases of the prolonged course of the pathological process, as compared with the common one, microorganisms exhibited hemolytic activity and high antilysozyme and anticomplementary levels. these findings served as t ...200415346941
intra-abdominal, retroperitoneal, and visceral abscesses in children.this review describes the microbiology, diagnosis, and management of intra-abdominal abscesses (including subphrenic, hepatic, splenic, and retroperitoneal abscesses) in children. they often occur as a complication of local or generalized peritonitis, commonly secondary to appendicitis, necrotizing enterocolitis, pelvic inflammatory disease, and tubo-ovarian infection, surgery or trauma. the original infection generally occurs because of the entry of enteric microorganisms into the peritoneal ca ...200415343468
relationship of subgingival and salivary microbiota to gingival overgrowth in heart transplant patients following cyclosporin a therapy.severe gingival overgrowth (go) is induced in patients taking cyclosporin a (csa) following organ transplantation. determining which patient will develop go is still not possible. the purpose of this study was to establish an association between csa and gingival overgrowth in heart transplant patients taking into account periodontal and microbiological conditions.200415341348
dna-dna relatedness and phylogenetic positions of slackia exigua, slackia heliotrinireducens, eggerthella lenta, and other related bacteria.recently, two asaccharolytic eubacterium species, eubacterium exiguum and eubacterium lentum, and peptostreptococcus heliotrinreducens have been reclassified as slackia exigua, eggerthella lenta and slackia heliotrinireducens in the novel genera on the basis of 16s rdna sequence analysis. but dna-dna relatedness among these species and other related bacteria have not been reported yet. dna-dna relatedness is the standard arbiter and the recommended method for the designation and evaluation of ne ...200415327650
microbiological profile of early onset/aggressive periodontitis patients.the objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence.200415327644
beta-lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis.this study assessed the extent of beta-lactamase-producing bacteria in subgingival plaque samples obtained from 25 patients with refractory marginal periodontitis in the usa. beta-lactamase-positive isolates were characterized using commercial diagnostic kits and partial sequencing of the 16s rrna gene. the susceptibilities to different antimicrobial agents were tested and, in addition, the isolates were screened for the presence of extended spectrum beta-lactamases (esbls). beta-lactamase-produ ...200415327642
initial antimicrobial effect of controlled-release doxycycline in subgingival determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp).200415324352
[bacterial infections in chronic otitis media--usefulness of molecular diagnostics based on pcr method].infections in chronic otitis media (com) are common, and identification of their pathogen is difficult, what is proven by high rate of negative results of bacteriological examinations. this fact may be explained by two factors: it is difficult to obtain a reliable material for culture and classic methods of bacteriological cultures may be of low sensitivity. amplification of bacterial dna in pcr is an examination, that leads to a higher effectiveness and sensitivity of bacteriological tests. the ...200415311593
crystal structure and biological implications of a bacterial albumin binding module in complex with human serum albumin.many bactericide species express surface proteins that interact with human serum albumin (hsa). protein pab from the anaerobic bacterium finegoldia magna (formerly peptostreptococcus magnus) represents one of these proteins. protein pab contains a domain of 53 amino acid residues known as the ga module. ga homologs are also found in protein g of group c and g streptococci. here we report the crystal structure of hsa in complex with the ga module of protein pab. the model of the complex was refin ...200415269208
[a case of infected emphysematous bulla successfully treated with percutaneous thoracic drainage].we present a case of infected emphysematous bulla successfully treated by percutaneous drainage. a 39-year-old man was admitted to our hospital because of fever and right lateral chest pain. a chest radiograph and ct revealed a cystic lesion with an air fluid level in the upper lobe of the right lung. the infected emphysematous bulla was not improved by the administration of antibiotics. on day 6 of hospitalization, a drainage tube (aspiration kit: 6 fr) was inserted percutaneously into the bull ...200415228143
chemotaxonomic analysis of bacterial populations colonizing the rectal mucosa in patients with ulcerative colitis.the etiology of ulcerative colitis (uc) is unknown, but evidence links it to bacteria belonging to the normal colonic microbiota. the aims of this study were to characterize bacteria colonizing the rectal epithelium, and to investigate whether significant differences existed in uc. rectal biopsy specimens were obtained via endoscopy from 9 patients with active colitis and 10 patients without inflammatory bowel disease. complex bacterial communities colonized the rectal mucosa in all subjects. ov ...200415227614
itih4 (inter-alpha-trypsin inhibitor heavy chain 4) is a new acute-phase protein isolated from cattle during experimental infection.we have isolated from calf serum a protein with an apparent m(r) of 120,000. the protein was detected by using antibodies against major acute-phase protein in pigs with acute inflammation. the amino acid sequence of an internal fragment revealed that this protein is the bovine counterpart of itih4, the heavy chain 4 of the inter-alpha-trypsin inhibitor family. the response of this protein in the sera was determined for animals during experimental bacterial and viral infections. in the bacterial ...200415213118
subgingival microbiota of chilean patients with chronic association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago.200415212354
whole-body autoradiography reveals that the peptostreptococcus magnus immunoglobulin-binding domains of protein l preferentially target b lymphocytes in the spleen and lymph nodes in vivo.protein l is an immunoglobulin (ig)-binding protein produced by the gram-positive bacterium peptostreptococcus magnus that interacts with the variable region of ig kappa light chains. the ig light chain-binding capacity of protein l gives it the potential to interact with cells expressing surface ig such as b cells. the present study was performed to address the in vivo trafficking of protein l at both the organ and the cellular level. using the powerful technique of whole-body autoradiography i ...200415186398
orbital cellulitis caused by peptostreptococcus. 200415184929
the bacteriology of the supragingival plaque of child dental patients in investigate the bacterial flora of the gingival plaques of pediatric dental patients in order to establish baseline data for future studies on the complex microbiota of the oral cavity of this group of child dental patients.200415181322
specific in vivo deletion of b-cell subpopulations expressing human immunoglobulins by the b-cell superantigen protein l.some pathogens have evolved to produce proteins, called b-cell superantigens, that can interact with human immunoglobulin variable regions, independently of the combining site, and activate b lymphocytes that express the target immunoglobulins. however, the in vivo consequences of these interactions on human b-cell numbers and function are largely unknown. using transgenic mice expressing fully human immunoglobulins, we studied the consequences of in vivo exposure of protein l of peptostreptococ ...200415155659
in vitro activities of the new semisynthetic glycopeptide telavancin (td-6424), vancomycin, daptomycin, linezolid, and four comparator agents against anaerobic gram-positive species and corynebacterium spp.telavancin is a new semisynthetic glycopeptide anti-infective with multiple mechanisms of action, including inhibition of bacterial membrane phospholipid synthesis and inhibition of bacterial cell wall synthesis. we determined the in vitro activities of telavancin, vancomycin, daptomycin, linezolid, quinupristin-dalfopristin, imipenem, piperacillin-tazobactam, and ampicillin against 268 clinical isolates of anaerobic gram-positive organisms and 31 corynebacterium strains using agar dilution meth ...200415155214
apical periodontitis development and bacterial response to endodontic treatment. experimental root canal infections in monkeys with selected bacterial six monkeys, 160 root canals were inoculated with a combination of four bacterial strains belonging to species streptococcus milleri, peptostreptococcus anaerobius, prevotella oralis, and fusobacterium nucleatum. in two other monkeys, 24 root canals were inoculated with a five-strain combination consisting of these strains and a strain of enterococcus faecalis. all strains were previously isolated from an infected monkey root canal. after 8-12 months, survival of the strains was recorded bact ...200415154917
changes in oral microbial profiles after periodontal treatment as determined by molecular analysis of 16s rrna genes.terminal rflp (t-rflp) analysis was used to investigate changes in the oral microbiota in saliva and subgingival plaque samples from one patient with aggressive periodontitis (subject a) and two patients with chronic periodontitis (subjects b and c) before and 3 months after periodontal treatment. substantial changes in the t-rflp patterns of subgingival plaque samples of subjects b and c were noted after 3 months of improved oral hygiene and full-mouth supra- and subgingival scaling and root pl ...200415150339
nitroimidazole resistance genes (nimb) in anaerobic gram-positive cocci (previously peptostreptococcus spp.).to investigate metronidazole resistance and the prevalence of nitroimidazole (nim) genes in clinically isolated anaerobic gram-positive cocci.200415150170
antibiotic resistance profile of the subgingival microbiota following systemic or local tetracycline therapy.tetracyclines have been extensively used as adjunctives to conventional periodontal therapy. emergence of resistant strains, however, has been reported. this study evaluated longitudinally the tetracycline resistance patterns of the subgingival microbiota of periodontitis subjects treated with systemic or local tetracycline therapy+scaling and root planing (srp).200415142209
systematic 16s rrna gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans.clinical microorganisms isolated during a 5-year study in our hospital that could not be identified by conventional criteria were studied by 16s rrna gene sequence analysis. each isolate yielded a > or =1,400-bp sequence containing <5 ambiguities which was compared with the genbank 16s rrna gene library; 1,404 such isolates were tested, and 120 were considered unique (27 isolates) or rare (< or =10 cases reported in the literature) human pathogens. eleven new species, "actinobaculum massiliae," ...200415131188
surveillance of susceptibility patterns in 1297 european and us anaerobic and capnophilic isolates to co-amoxiclav and five other antimicrobial vitro susceptibility data were collected for co-amoxiclav and other antimicrobial agents against 1297 recent anaerobe isolates collected in europe and the usa. the co-amoxiclav (amoxicillin/clavulanic acid) mic(50/90)s (amoxicillin/clavulanic acid concentration in a ratio of 2:1, expressed in terms of amoxicillin concentration in mg/l) were 0.5/4 for bacteroides fragilis, </=0.125/1 for prevotella species, </=0.125/0.25 for fusobacterium nucleatum, 0.5/1 for eikenella corrodens, 0.25/8 for pe ...200415128729
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2002 and march 2003].tendency of isolated bacteria from infections in general surgery during the period from april 2002 to march 2003 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 334 strains were isolated from 131 (75.3%) of 174 patients with surgical infections. one hundred and seventy-one strains were isolated from primary infections, and 163 strains were isolated from post-operative infections. from primary infections, anaerobic gram-positive bacteria ...200415116573
the microbial synergy of peptostreptococcus micros and prevotella intermedia in a murine abscess model.this study characterized the microbial interaction of peptostreptococcus micros and prevotella intermedia, the major pathogens of dentoalveolar infection, using a murine model. subcutaneous injection of p. micros cells in the dorsum of the mouse together with living cells of p. intermedia resulted in a significantly larger abscess when compared with single injection of the organisms (p < 0.02). the abscess size was also significantly increased (p < 0.05) when the plate-cultured cell suspension o ...200415107069
septic arthritis after arthroscopic anterior cruciate ligament reconstruction.a retrospective review of postoperative infected anterior cruciate ligament (acl) reconstruction was done on 472 consecutive cases in one institution. the purpose was to assess the incidence, diagnosis, treatment and outcome factors.200415098639
radiology quiz case 2. cervical necrotizing fasciitis (cnf), odontogenic origin. 200415096437
down-modulation of the antigen receptor by a superantigen for human b cells.b cell superantigens (sags) have been implicated in human diseases by demonstrating non-clonotypic expansion of b cells bearing certain immunoglobulin variable region genes. one possibility is that, during infection with microorganisms secreting sags, these potent molecules might modulate bcr expression. to test this hypothesis, we investigated the potential effects of a sag, protein l from peptostreptococcus magnus, on antigen b cell receptor (bcr) surface expression in vitro. using fluorescenc ...200415081532
microbiology of infected hemangiomas in children.bacterial infections are a common complication of hemangiomas in children. the objective of this study was to establish the aerobic and anaerobic microbiology of infected hemangiomas. a retrospective 8-year review of clinical and microbiology laboratory records from patients with secondarily infected hemangiomas was carried out. specimens from infected sites were processed for the presence of aerobic and anaerobic bacteria. bacterial growth was present in 32 of 38 specimens. aerobic bacteria alo ...200415078348
[a bacteriology analysis from infected root canals of human deciduous teeth]objective: to provide a basis for therapy and prevention, the flora of infected root canals of human deciduous teeth was analyzed.methods: by immediate-enzyme method, the bacteria compositions of 22 cases with infected root canals of deciduous teeth, including 8 cases with acute periapical inflammation and 14 with chronic ones, were examined. results: among 240 strains bactera, 200 strains were obligate anaerobes, belonging to the genera peptostreptococcus, bacteriodes, veillonella, eubacterrum, ...199815071650
septic arthritis of the hip and intrapelvic abscess following intra-articular injection of hylan g-f 20. a case report. 200415069151
comparative analysis of the four rrna operons in finegoldia magna atcc29328.there are four rrna operons rrna, rrnb, rrnc and rrnd on the genome of finegoldia magna (formerly peptostreptococcus magnus) atcc29328, which, in contrast to those of clostridia, are dispersed around the chromosome. using a bac library we determined the nucleotide sequences and structures of all four operons, including their flanking regions, and performed comparative analyses. we identified putative boxa sequences in the operons, which should be required for rrna transcription antitermination, ...200415053317
phospholipid analogue profiles of peptostreptococcus, micromonas, and finegoldia species analysed by fast atom bombardment mass spectrometry.species of peptostreptococcus cause a variety of infections, primarily abscesses of soft tissues, joints, and mucous membranes. the aim of this study was to compare the phospholipid analogue profiles of peptostreptococcus species, represented by p. anaerobius, p. asaccharolyticus, p. indolicus, p. lacrimalis, and p. prevotii; micromonas micros (p. micros) and finegoldia magna (p. magnus). after anaerobic growth on blood-faa, lipids extracted by chloroform-methanol (2:1 v/v) were purified, then a ...200115049457
[anaerobes analysis in 80 cases with ora maxillofacial infections]objective:to define the infection types of 80 cases of ora-maxillofacial infection and investigate its anaerobes' distribution and the role of the anaerobes' metabolic products in their identification.methods:we isolated and cultured anaerobes from the purulent specimen and applied the gasliquid chromatograph (glc) technique to analyze the anaerobes' metabolic products to define their genera and species in the meantime.results:bacteria were isolated from all of the purulent specimen.isolation ra ...199915048214
cervical spinal epidural abscess and meningitis due to prevotella oris and peptostreptococcus micros after retropharyngeal surgery. 200415034651
characteristics of anaerobes from skin specimens.anaerobes isolated from skin specimens from 1999 to 2001 were examined. the most common type was peptostreptococcus spp., especially p. magnus and p. assaccharolyticus and bacteroides fragilis. dominance was seen for p. magnus, p. acnes and p. prevotii. peptostreptococcus spp. and p. acnes showed high susceptibility to four antimicrobial agents. prevotella spp. and b. fragilis showed low or no susceptibility to ampicillin, while b. fragilis showed low susceptibility to ceftizoxime. evaluation of ...200315018306
[antibacterial activity of oral cephems against various clinically isolated strains].we determined the antibacterial activities of oral cephems against isolated from the patients with the respiratory infections, the urinary tract infections, and infections in the obstetrics field of an adult and a child, during the period from 2002 to 2003; streptococcus pyogenes, streptococcus pneumoniae, haemophilus influenzae, branhamella catarrhalis, klebsiella pneumoniae and escherichia coli of 40 strains of each, and peptostreptococcus spp. 22 strains. s. pneumoniae and h. influenzae strai ...200315007876
[surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2002].the antibacterial activity of cefmetazole (cmz) against clinical isolates from 15 medical institutions all over japan was evaluated yearly for two years from june 2000 to march 2002 and compared with that of other parenteral beta-lactams, cefazolin (cez), cefotiam (ctm), sulbactam/cefoperazone (sbt/cpz), and flomoxef (fmox). in the first surveillance from june 2000 to march 2001, 575 isolates of 13 species were tested, and 548 isolates of the same 13 species were tested in the second surveillanc ...200315007873
human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms.human beta-defensins 2 and 3 (hbd-2 and hbd-3) are inducible peptides present at sites of infection in the oral cavity. a few studies have reported broad-spectrum antimicrobial activity for both peptides. however, no comprehensive study has thoroughly investigated their potential against oral pathogens. the purpose of this study was to test the effectiveness of hbd-2 and hbd-3 against a collection of oral organisms (actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gin ...200415004048
in vivo vl-targeted activation-induced apoptotic supraclonal deletion by a microbial b cell interfere with host immune responses, some microbial pathogens produce proteins with the properties of superantigens, which can interact via conserved v region framework subdomains of the ag receptors of lymphocytes rather than the complementarity-determining region involved in the binding of conventional ags. in recent studies, we have elucidated how a model b cell superantigen affects the host immune system by targeting a conserved v(h) site on the ag receptors of b lymphocytes. to determin ...200414978088
microbiological examination of infected dental root canals.the aim of this study was to investigate the root canal microbiota of primary and secondary root-infected canals and the association of constituent species with specific endodontic signs and symptoms.200414871344
presurgical antimicrobial prophylaxis: effect on ocular flora in healthy patients.evaluation of presurgical antimicrobial prophylaxis for reduction of ocular flora.200414756385
bacteremia following surgical dental extraction with an emphasis on anaerobic strains.our aim was to investigate bacteremia caused by surgical extraction of partly erupted mandibular third molars. from 16 young adults, bacterial samples were taken from the third-molar pericoronal pocket and post-operatively from the extraction socket, and blood samples were drawn from the ante-cubital vein up to 30 min after surgery. of the subjects, 88% had detectable bacteremia-50% 1 min after the incision, 44% immediately after extraction. the respective percentages at 10, 15, and 30 min were ...200414742658
antibiotic susceptibility of bacterial isolates in gingivitis and periodontitis.the present study was conducted to isolate aerobic as well as anaerobic microbes from patients of gingivitis and adult periodontitis and to study the susceptibility patterns of these isolates to different antibiotics. in our study all the samples belonging to the control as well as the study groups yielded microbes. aerobes and facultative anaerobes were isolated from 100 percent and 96 percent cases of normal gingiva and orodental infections respectively. anaerobes were isolated from 80 percent ...200314705453
inflammatory polyp in the middle ear with secondary suppurative meningoencephalitis in a cat.a 15-month-old male maine coon cat presented with persistent auricular discharge and progressive head tilt, ataxia, and loss of blink on the right side. using computed tomography a hyperattenuating, contrast-enhancing material within a thickened right tympanic bulla and contrast enhancement of the adjacent cerebellum were identified. marked suppurative inflammation was identified on cerebrospinal fluid analysis with no growth on bacterial culture. ventral bulla osteotomy was performed to remove ...200314703245
bacterial artificial chromosome library of finegoldia magna atcc 29328 for genetic mapping and comparative genomics.we constructed a bacterial artificial chromosome (bac) library of finegoldia magna atcc 29328 dna to facilitate further genome analysis of f. magna. the bac library contained 385 clones with an average insert size of 55 kb, representing a 10.1-fold genomic coverage. repeated dna hybridization using primer sets designed on the basis of bac-end sequences yielded nine contigs covering 95% of the chromosome and two contigs covering 98% of the plasmid. the contigs were localized on the physical map o ...200314695451
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--i. gram-positive bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, and carbapenems. changes in the bacterial susceptibility for czop were also evaluated with the resistance ratio calculated with breakpoint mic. sixteen species (2,363 strains) of gram-positive bacteria were isolated from the clinical materials annually collected ...200314692380
observation and characterization of the interaction between a single immunoglobulin binding domain of protein l and two equivalents of human kappa light chains.detailed stopped-flow studies in combination with site-directed mutagenesis, isothermal titration calorimetry data and x-ray crystallographic knowledge have revealed that the biphasic pre-equilibrium fluorescence changes reported for a single ig-binding domain of protein l from peptostreptococcus magnus binding to kappa light chain are due to the binding of the kappa light chain at two separate sites on the protein l molecule. elimination of binding site 2 through the mutation a66w has allowed t ...200414668335
frozen cancellous bone allografts: positive cultures of implanted grafts in posterior fusions of the spine.we have carried out a study on the behaviour pattern of implanted allografts initially stored in perfect conditions (aseptically processed, culture-negative and stored at -80 degrees c) but which presented positive cultures at the implantation stage. there is no information available on how to deal with this type of situation, so our aim was to set guidelines on the course of action which would be required in such a case. this was a retrospective study of 112 patients who underwent a spinal arth ...200414648307
fak deficiency in cells contributing to the basal lamina results in cortical abnormalities resembling congenital muscular dystrophies.targeted deletion of focal adhesion kinase (fak) in the developing dorsal forebrain resulted in local disruptions of the cortical basement membrane located between the neuroepithelium and pia-meninges. at disruption sites, clusters of neurons invaded the marginal zone. retraction of radial glial endfeet, midline fusion of brain hemispheres, and gliosis also occurred, similar to type ii cobblestone lissencephaly as seen in congenital muscular dystrophy. interestingly, targeted deletion of fak in ...200314642275
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