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microbiology and antibiotic sensitivities of head and neck space infections of odontogenic origin.the purpose of this study is to assess the anatomical spaces and causative micro-organisms responsible for deep fascial space head and neck infections and evaluate the resistance of antibiotics used in the treatment of these infections.200616916672
microbiology of intracranial abscesses associated with sinusitis of odontogenic origin.the unique microbiology of sinusitis of dental origin that is associated with intracranial abscesses (ias) and the correlation between the organisms at the two sites has not been reported before. this report describes the author's experience during a 30-year period in studying the microbiology of 8 ias and their corresponding sinusitis of dental origin.200617214267
evaluation of subgingival bacteria in the dog and susceptibility to commonly used antibiotics.the aim of the present investigation was to evaluate the subgingival aerobic and anaerobic flora of 13 dogs with periodontal disease and the susceptibility of these bacteria to antibiotics currently approved in italy for treatment of canine infections. of the anaerobic bacteria, bacteroides fragilis was most frequently isolated, followed by peptostreptococcus + porphyromonas gingivalis and prevotella intermedia. of the aerobic bacteria, alpha-hemolytic streptococcus was most frequently isolated, ...200617286127
[a case of multiple lung abscesses successfully treated with computed tomography guided percutaneous thoracic drainage].a 48-year-old woman who had abused alcohol admitted to our hospital because of fever and chest pain. laboratory data presented inflammation and liver dysfunction. chest x-ray films revealed multiple mass lesions with air fluid levels. we diagnosed multiple lung abscesses and started treatment with antibiotics. however, her fever did not improve and mass lesions increased. on day 6 of hospitalization, three drainage tubes were inserted percutaneously into the mass lesions under computed tomograph ...200616972615
photodynamic therapy for endodontic disinfection.the aims of this study were to investigate the effects of photodynamic therapy (pdt) on endodontic pathogens in planktonic phase as well as on enterococcus faecalis biofilms in experimentally infected root canals of extracted teeth. strains of microorganisms were sensitized with methylene blue (25 microg/ml) for 5 minutes followed by exposure to red light of 665 nm with an energy fluence of 30 j/cm2. methylene blue fully eliminated all bacterial species with the exception of e. faecalis (53% kil ...200616982278
in vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections.against 443 aerobic and anaerobic bacteria isolated from diabetic foot infections, ceftobiprole mics (microg/ml) at which 90% of the isolates tested were inhibited were as follows: methicillin-resistant staphylococcus aureus, 1; methicillin-susceptible s. aureus and staphylococcus lugdunensis, 0.5; anaerococcus prevotii, 0.125; finegoldia magna, 0.5; peptoniphilus asaccharolyticus, 1; peptostreptococcus anaerobius, 4; escherichia coli and enterobacter species, 0.125; klebsiella species, 2; and p ...200616982780
root canal microbiota of dogs' teeth with periapical lesions induced by two different methods.the microbial composition was investigated in root canals of dogs' teeth with periapical lesions induced by 2 different methods: open versus sealed canals.200616997126
red autofluorescence of dental plaque bacteria.red autofluorescence of plaque and its relation to fluorescence of a single species in the biofilm was studied. fluorescence images of non-disclosed and disclosed plaque of 28 first-year students were captured. the plaque samples were assessed by culture methods and studied for red autofluorescence. species capable of producing red autofluorescence were cultivated from subjects with and without red plaque autofluorescence. red autofluorescence was observed from actinomyces odontolyticus, prevote ...200617063027
suppurative thyroiditis. 200617098092
a novel modification of omental transposition to reduce the risk of gastrointestinal herniation into the chest. 200616932176
[diagnosis and treatment of a liver abscess with contrast-enhanced ultrasound].we report on a patient with pyogenic liver abscess. using a ultrasound contrast agent improves the characterization of pyogenic hepatic abscesses and facilitates repetitive therapeutic punctures. the use of ultrasound contrast agents opens new aspects in the diagnosis of liver abscesses and gives additional confidence in diagnostic and therapeutic procedures.200616939125
periodontal status in smokers and never-smokers: clinical findings and real-time polymerase chain reaction quantification of putative periodontal pathogens.smoking is a well-known risk factor for destructive periodontal disease, but its relationship with periodontal status and subgingival microbiota remains unclear. inherent limitations of microbiological methods previously used may partly explain these mixed results, and real-time polymerase chain reaction (pcr) has been presented as a valid alternative. the aim of the present study was to investigate the clinical condition and microbiological profile of patients with chronic periodontitis as rela ...200616945023
changes in bacterial profile during amebiasis: demonstration of anaerobic bacteria in ala pus samples.little is known about the changes in gut resident flora during amebic colitis and amebic liver abscess (ala) caused by entamoeba histolytica infection. fecal samples from ala patients, from healthy e. histolytica negative and positive (asymptomatic) individuals, and from pre- and post-metronidazole-treated healthy volunteers and pus samples from ala patients were tested for the presence of various bacterial genera using 16s rrna-based primers. statistically significant reduction in lactobacillus ...200617123981
[comparative analyses of colonization degree of oral cavity by microbial flora during application of teeth prosthetic devices].the degree of adhesion and colonization was studied among 27 patients with generalized paradontitis of moderate severity and in remission stage. all these patients had different material teeth prostheses. 12 patients had acrylic plastic "ftorax" material prostheses and 15 of them polypropylenes prostheses. prostheses prepared from acryl plastic were intensively colonized by stable microflora of oral cavity, as well as by paradontopathogens, whereas usage of polypropylenes prostheses is rather ef ...200616905807
influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth.the purpose of this study was twofold: first, to determine the influence on the healing of the periapical tissues when selected bacterial strains and combinations thereof remain after root canal treatment; and, second, the relationship to healing of the quality of the root filling. in eight monkeys, 175 root canals, previously infected with combinations of four or five bacterial strains and with radiographically verified apical periodontitis, were endodontically treated, bacteriologically contro ...200616911098
contribution of proteases and plasmin-acquired activity in migration of peptostreptococcus micros through a reconstituted basement membrane.peptostreptococcus micros is a gram-positive bacterium that has been associated with chronic periodontitis and endodontic infections. the aims of this study were to investigate the production of proteases and the acquisition of plasmin activity by rough and smooth morphotypes of p. micros. the contribution of these properties in the migration of bacteria through a reconstituted basement membrane was also evaluated.200616922932
aerobic and anaerobic bacteria in subgingival and supragingival plaques of adult patients with periodontal disease.clinical, epidemiological and microbiological examinations of adult patients with periodontal disease.200617458065
bacterial composition in the supragingival plaques of children with and without dental caries.the purpose of the present investigation was to determine if the supragingival bacterial composition plaques in children with caries would differ from those found in caries-free controls.200617458088
bacteriology of chronic sinusitis and acute exacerbation of chronic sinusitis.to establish the microbiological characteristics of acute exacerbation of chronic sinusitis (aecs).200617043258
microorganisms in root carious lesions in adults.root caries is emerging as a significant problem in the middle aged and elderly subjects because of the improving general health conditions, and medical and technological advances. the purpose of this investigation was to assess the prevalence of aerobic and anaerobic bacteria as well as yeasts of candida genus in root carious lesions in middle-aged and older adults.200617460839
microbiological and immunological study of staphylococcus vaccine effects in periodontitis.the aim of this study was to evaluate the immunomodulatory effect of the staphylococcal vaccine inoculated subcutaneously in 15 patients with chronic periodontitis. bacteriological investigation of samples collected from the periodontal pocket for aerobic and anaerobic microorganisms was performed by classic bacteriological procedures before and after vaccination. the following immune system parameters were evaluated: c reactive protein (crp), serum level of c3 complement fraction, igg, iga, and ...200517405309
in vitro activity of oritavancin (ly333328), vancomycin, clindamycin, and metronidazole against clostridium perfringens, propionibacterium acnes, and anaerobic gram-positive cocci.using an agar dilution method, we determined the in vitro activity of oritavancin, vancomycin, clindamycin and metronidazole against 114 unique clinical isolates of gram-positive anaerobes. mic(90)s (microg/ml) for oritavancin were as follows: clostridium perfringens 1.0, propionibacterium acnes 0.25, peptostreptococcus anaerobius 0.25, peptoniphilus asaccharolyticus 0.5, finegoldia magna 0.25, micromonas. micros 0.25, and anaerococcus prevotii 0.25. on a weight basis, oritavancin is slightly mo ...200516701537
[bacterial spectra and antibiotics in odontogenic infections. renaissance of the penicillins?].the role played by odontogenic infection in dental, oral, and maxillofacial surgery is not to be underestimated even at the present time. an extensive, standardized, prospective study was performed with the intention of verifying the bacterial spectrum of odontogenic infections to evaluate antibiotic sensitivity.200516261393
the effect of haart on salivary microbiota in the women's interagency hiv study (wihs).study the prevalence of potentially pathogenic microorganisms in saliva of hiv-positive women in the women's interagency hiv study.200516301151
microbiology of intracranial abscesses and their associated sinusitis.to evaluate the organisms recovered from infected sinuses and associated intracranial abscesses (ias).200516301376
microbial analysis of the autogenous bone collected by bone filter during oral surgery: a clinical study.the purposes of the study presented are to identify the microbial content of the collected bone debris and to determine the antibacterial efficiency of chlorhexidine mouth rinse in reducing the microbial content of the collected bone debris.200516243175
[severe pelvic inflammatory disease. response to ertapenem].pelvic inflammatory disease (pid) is an important health problem. delay in diagnosis and effective treatment contributes to inflammatory sequelae with risk of future fertility problems and chronic pelvic pain. treatment regimens must provide empiric, broad-spectrum coverage of likely pathogens but also take into account patient's risk profile. in this article we report the case of a young woman affected by a rare genital malformation who required the creation of a neovagina made of abdominal ski ...200516446794
[two cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae].most of bartholin's gland abscesses have been thought to be caused by microorganisms found in opportunistic infections. however, we have encountered two very interesting cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae, two major pathogens of respiratory tract infections. in the first case, since abscess formation was not observed due to disintegration, cefdinir (cfdn), 300 mg/day, t.i.d. for 5 days was administered. the treatment improved clinic ...200516276738
[nationwide surveillance of parenteral antibiotics containing meropenem activities against clinically isolated strains in 2004].the antibacterial activity of meropenem (mepm) and other parenteral antibiotics against clinical isolates of 907 strains of gram-positive bacteria, 1790 strains of gram-negative bacteria, and 192 strains of anaerobic bacteria obtained from 30 medical institutions during 2004 was measured. the results were as follows; 1. mic90 of mepm for almost all of enterobacteriaceae and haemophilus influenzae were 4-fold to 32-fold lower than those of other carbapenems. mepm was more active than other carbap ...200516521347
effect of sodium alendronate on alveolar bone resorption in experimental periodontitis in rats.bisphosphonates are potent inhibitors of bone resorption and were shown to inhibit bone resorption in experimental periodontitis by unknown mechanisms. we studied the effect of the aminobisphosphonate sodium alendronate (sa) in experimental periodontitis. wistar rats were subjected to ligature placement around the second upper left molars.200516274309
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004].tendency of isolated bacteria from infections in general surgery during the period from april 2003 to march 2004 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 455 strains including 14 strains of candida spp. were isolated from 191(75.2%) of 254 patients with surgical infections. two hundred and thirty-nine strains were isolated from primary infections, and 216 strains were isolated from postoperative infections. from primary infection ...200515997655
on the origin of the thermostabilization of proteins induced by sodium phosphate.the b1 domain of protein l shows a linear rise in thermostability with increasing concentrations of sodium phosphate. equal behavior is observed for a set of mutant proteins where surface lysines are mutated to noncharged residues, but the mutant's thermostabilities show different sensitivities to phosphate, encoded in the varying slopes observed (mi). the melting temperature in the absence of the cosolute also correlates linearly with mi. the stabilizing effect of the phosphate ion reaches a sa ...200515998065
antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in the netherlands and spain.antimicrobial resistance of periodontal pathogens towards currently used antibiotics in periodontics has been investigated in a previous study. microbial resistance in the periodontal microflora was more frequently observed in spanish patients in comparison with dutch patients. the aim of the present study was to compare antimicrobial susceptibility profiles of five periodontal bacteria isolated from periodontitis patients in spain and in the netherlands.200515998275
toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria.the colonic epithelium provides an interface between the host and micro-organisms colonising the gastrointestinal tract. molecular recognition of bacteria is facilitated through toll-like receptors (tlr). the colonic epithelium expresses relatively high levels of mrna for tlr3 and less for tlr2 and -4. little is known of the expression patterns and mode of induction of expression for these pattern recognition receptors in human colon. the aim of this study was to investigate their localization i ...200516011525
[comparison of the detection of anaerobic bacteria in haemoculture systems bactec lytic/10 anaerobic/f, bact/alert fan anaerobic and bact/alert fn anaerobic].the purpose of this study is to deal with the problem of anaerobic cultivation of clinical specimens and consider the possibility of using semi-automated blood culture instruments. the bactec lytic, bact/alert fan anaerobic and bact/alert fn anaerobic bottles were inoculated with bacteriodes fragilis, clostridium perfringens, peptostreptococcus anaerobius and feingoldia magna strains. the times to detection (ttd) for positive bottles were evaluated with reference to the number of inoculated bact ...200516025428
coaggregation profiles of the microflora from root surface caries lesions.bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. as no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single candida spp. the quantitative coa ...200515598414
tobacco smoking and periodontal microflora in a saudi arabian population.to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits.200515882210
the role of bacterial interference in otitis, sinusitis and tonsillitis.bacterial interactions that include antagonism (interference) and synergism help maintain balance between the members of the normal endogenous flora. alpha-streptococci that predominate in the normal respiratory tract flora attracted most attention in studies of bacterial interference. other organisms that possess interfering characteristics in upper respiratory tract infections (urtis) are nonhemolytic streptococci, and prevotella and peptostreptococcus spp. the production of bacteriocins by so ...200516025067
[the occurrence of microorganisms in intraoral abscesses].the aim of this study was to assess the occurrence of microorganisms in 39 intraoral abscesses. the samples were place in transport medium. the materials were inoculated on adequate enrichment and selective media and cultivated under anaerobic and aerobic conditions. the microorganisms were found in all samples (100%), anaerobic bacteria most frequently were recovered. the predominant anaerobes were from genus prevotella, bacteroides, fusobacterium and peptostreptococcus. among aerobic bacteria, ...200516134393
microbiology of acute exacerbation of chronic sinusitis.we undertook to evaluate the microbiology of acute exacerbation of chronic sinusitis (aecs).200516134356
trypanosoma cruzi: influence of predominant bacteria from indigenous digestive microbiota on experimental infection in mice.to verify the influence of some predominant components from indigenous microbiota on systemic immunological responses during experimental chagas disease, germ-free nih swiss mice were mono-associated with escherichia coli, enterococcus faecalis, bacteroides vulgatus or peptostreptococcus sp. and then infected with the y strain of trypanosoma cruzi. all the mono-associations predominantly induced a th1 type of specific immune response to the infection by t. cruzi. a direct correlation was observe ...200516038901
identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis.most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ...200516081935
[efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study].erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens.200516087299
antibacterial activity of aqueous extracts of selected chewing sticks.this aim of this study was to determine the antibacterial activity in extracts obtained from various nigerian chewing sticks. aqueous extracts from seventeen chewing sticks and the fruit of c. ferruginea, one fruit used in oral hygiene in nigeria, were screened for antibacterial activity against type cultures of staphylococcus aureus, bacillus subtilis, escherichia coli, and pseudomonas aeruginosa. eleven of the test extracts showed activity against at least two of these referenced organisms. mi ...200516127476
[role of obligate anaerobes in infections in hospitalized patients and therapeutic options].monitoring of prevalence and antibiotic susceptibility of strictly anaerobic bacteria, causing infections in hospitalized patients, constitutes a part of a program for prudent use of antibiotics. the aim of the study was to assess prevalence of strictly anaerobic bacteria in patients hospitalized in a tertiary care hospital in 2001-2002 with reference to empiric antibiotic therapy. the most common gram-positive bacteria were clostridium difficile--27.7%, peptostreptococcus spp. and peptoniphilus ...200516134392
intestinal bacterial communities that produce active estrogen-like compounds enterodiol and enterolactone in humans.lignans are dietary diphenolic compounds which require activation by intestinal bacteria to exert possible beneficial health effects. the intestinal ecosystem plays a crucial role in lignan metabolism, but the organisms involved are poorly described. to characterize the bacterial communities responsible for secoisolariciresinol (seco) activation, i.e., the communities that produce the enterolignans enterodiol (ed) and enterolactone (el), a study with 24 human subjects was undertaken. seco activa ...200516204524
chronic sinusitis in children and adults: role of bacteria and antimicrobial management.the nasopharynx serves as the reservoir for anaerobic bacteria as well as pathogenic bacteria that can cause respiratory infections including sinusitis. some of these organisms possess the ability to interfere with the growth of potential pathogens and may play a role in preventing infections. anaerobic bacteria emerge as pathogens as the infection becomes chronic. this may result from the selective pressure of antimicrobial agents that enable resistant anaerobic organisms to survive, and from t ...200516216174
[soft tissue infections in oral, maxillofacial, and plastic surgery. bacterial spectra and antibiotics].soft tissue infections in the maxillofacial region are mainly caused by staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli but also by members of the genera enterococcus, klebsiella, and enterobacter, respectively.200516228187
a case of cutaneous odontogenic sinus.despite the fact that cutaneous sinus tracts of odontogenic origin are well documented, the condition is still commonly misdiagnosed, because chronic periapical periodontitis may be asymptomatic and is rarely open to the skin. a 75-year-old japanese woman presented to our clinic with the chief complaint of a left cheek skin lesion with mild pain. physical examination revealed a subcutaneous nodule covered with erythematous skin on her left buccal region. cultures from the subcutaneous nodule gre ...200516361742
infected pneumatocele following anaerobic pneumonia in adult.we report a case of an infected pneumatocele in the course of anaerobic pneumonia in an adult. to the best of our knowledge, anaerobic pneumonia complicated by a pneumatocele in an adult has not previously been described. the pneumatocele occurred on the fifth day of hospitalization, and rapidly increased in size, with the development of a subsequent mixed anaerobe infection. a pig-tail catheter was inserted and the pus drained. the bacterial culture from the pus was positive for three anaerobes ...200516491835
bacterial etiology of diabetic foot infections in south india.foot infections are a frequent complication of patients with diabetes mellitus, accounting for up to 20% of diabetes-related hospital admissions. infectious agents are associated with the worst outcomes, which may ultimately lead to amputation of the infected foot unless prompt treatment strategies are ensued. the present study sought to reveal the bacterial etiology of diabetic foot ulcers in south india, the diabetic capital of india.200516314237
microbiology profile in women with pelvic inflammatory disease in relation to iud use.to study the microbial characteristics of patients with pelvic inflammatory disease (pid) and the possible impact of an intrauterine device (iud) on the microbial environment in women presenting with pid.200516338777
[bacterial 16s rdna sequence analysis of siberian tiger faecal flora].bacterial 16s rdna library of siberian tiger was developed and 15 different clones were obtained using ecor i and hind iii in restriction fragment length polymorphism analysis. dna sequencing and similarity analysis showed that 10 clones matched corresponding clostridium sequences, of which 6 sequences had over 99% similarity with clostridium novyi type a, and 4 sequences had 97% similarity with swine manure bacterium rt-18b, which identified as peptostreptococcus spp. the other five 16s rdna se ...200516342753
effect of mineral trioxide aggregate on cytokine production by peritoneal macrophages.to test the effect of two commercial brands of grey mineral trioxide aggregate (proroot and mta-angelus) on cytokine production by m1 and m2 inflammatory macrophages.200516343117
bacteriological study of paediatric and adult chronic suppurative otitis media.chronic suppurative otitis media (csom) is one of the commonest illnesses in ent practice which requires medical attention all the more in children of poor socio-economic status having in past inadequate treatment and negligent medical care. the present study was conducted to find out the various aerobic and anaerobic microorganisms associated with csom in paediatric and adult cases and their current antimicrobial susceptibility pattern as a guide to therapy. samples were collected from 109 clin ...200516761774
microbiology and management of infected neck cysts.to review and describe the microbiology, diagnosis, and management of infected neck cysts.200515742293
[pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology].this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria.200515757581
detection of bacteria in platelet concentrates: comparison of broad-range real-time 16s rdna polymerase chain reaction and automated culturing.based on real-time polymerase chain reaction (pcr) technology, a broad-range 16s rdna assay was validated and its performance was compared to that of an automated culture system to determine its usefulness for rapid routine screening of platelet concentrates (pcs).200515847662
periodontal infections and pre-term low birth weight: a case-control study.pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ...200515691348
b cell superantigens: a microbe's answer to innate-like b cells and natural antibodies.marginal zone b cells and b-1 cells have been termed innate-like b cells as they express limited repertoires that play special roles in immune defenses against common infections. these b cells are the sources of natural antibodies and are capable of highly accelerated clonal responses that help counter blood-borne infections. we have characterized a class of microbial product with highly adapted binding interactions with host immunoglobulins/b cell receptors (bcrs), which enable the targeting of ...200515633015
traumatic cholesteatoma presenting as a brain abscess: a case report.to discuss the clinical aspects and management of traumatic cholesteatomas of the temporal bone.200515699721
bacteriology of chronic dacryocystitis in a tertiary eye care center.to determine the current bacteriology of adult chronic dacryocystitis in a tertiary eye care center and compare it with previously reported studies.200515942496
recovery of anaerobic bacteria from wounds after lawn-mower injuries.accidental injury while using lawn mowers can cause serious infectious complications in the injured extremity. anaerobic bacteria were rarely recovered from this infection. two children who sustained injury in their foot by a lawn mower developed severe wound infection. culture of the wound from 1 patient had heavy growth of clostridium bifermentans and peptostreptococcus magnus, and the culture from the other child grew clostridium perfringens. antimicrobial therapy directed at the pathogens an ...200515699821
pharmacokinetics and pharmacodynamics of linezolid in obese patients with cellulitis.linezolid is an oxazolidinone antimicrobial with excellent oral bioavailability and tissue penetration and is active against multidrug-resistant skin/soft tissue pathogens.200515701775
influence of antimicrobial treatment for helicobacter pylori infection on the intestinal microflora in japanese macaques.eradication treatment for helicobacter pylori is known to cause mild but relatively frequent adverse effects. some adverse effects such as diarrhoea and soft stools are related to disruption of the composition of the intestinal microflora. this study investigated the microfloral changes resulting from administration of an eradication regimen using proton pump inhibitor (ppi), amoxycillin and clarithromycin. twenty-eight laboratory-bred japanese macaques either were administered eradication treat ...200515713617
micromonas (peptostreptococcus) micros: unusual case of prosthetic joint infection associated with dental procedures.micromonas (peptostreptococcus) micros is frequently associated with periodontal disease as well as respiratory, gastrointestinal and female genitourinary tract infections, but only rarely has been reported as a pathogenic agent of prosthetic joint infections. here we describe a case of a 63-year-old woman with prosthetic joint infection of total hip arthroplasty caused by the anaerobic species micromonas micros, associated with tooth extraction. samples obtained intraoperatively and from the or ...200515715175
binding of actinobacillus actinomycetemcomitans lipopolysaccharides to peptostreptococcus micros stimulates tumor necrosis factor alpha production by macrophage-like cells.peptostreptococcus micros is a gram-positive bacterium that has been associated with periodontitis and endodontic infections. in this study, we hypothesized that p. micros binds the immunomodulating component lipopolysaccharide derived from gram-negative bacteria to increase its capacity to stimulate cytokine production by host cells. the ability of p. micros to bind actinobacillus actinomycetemcomitans lipopolysaccharide was demonstrated by an enzyme-linked immunosorbent assay and by immunoelec ...200515720573
properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an escherichia coli host.peptostreptococcus asaccharolyticus glutamate dehydrogenase (l-glutamate: nad+ oxidoreductase (deaminating); ec 1.4.1.2) overexpressed in escherichia coli has been purified by two new methods. enzyme made by the first method showed remarkable thermophilicity, with a temperature optimum of 60 degrees c, and also thermostability, which suggested the second, simpler method, incorporating a heat step. this produced 94 mg of homogeneous protein per litre culture medium. the basic kinetic parameters f ...200515727821
bacteriology of acute and chronic ethmoid sinusitis.aspirates from 26 acutely and 17 chronically infected ethmoid sinuses were studied. thirty-seven aerobes and 10 anaerobes were recovered from isolates from patients with acute sinusitis. streptococcus pneumoniae and haemophilus influenzae were predominant. twenty-seven aerobes and 41 anaerobes were found in isolates from patients with chronic sinusitis. the predominant isolates were anaerobic gram-negative bacilli and peptostreptococcus spp.200516000483
chicken anti-protein l for the detection of small amounts of protein l in the presence of igg.protein l is a cell surface protein, expressed by peptostreptoccocus magnus, which binds to the variable light chains of immunoglobulins without interfering with antigen binding. it can be used for purification of mammalian antibodies of all classes in contrast to the ig-binding proteins protein a and protein g. detection of protein l leakage into antibody preparations is important, since protein l could interfere in immunological assays and cause adverse reactions in vivo. here we have develope ...200515857176
microbiology of acute and chronic maxillary sinusitis associated with an odontogenic origin.: to study the microbiology of sinusitis associated with odontogenic origin.200515867647
engineering high affinity superantigens by phage display.protein l (ppl) is a b-cell superantigen from peptostreptococcus magnus known to bind to mammalian vkappa light chains. ppl from p.magnus strain 312 comprises five homologous immunoglobulin (ig) binding domains. we first analysed the binding of the individual domains (b1-b5) of ppl(312) to human vkappa light chains (huvkappa) subtypes 1 (huvkappai) and 3 (huvkappaiii). using a combination of rational design and phage selection we isolated mutants of the n-terminal b1 domain with a 14-fold increa ...200515733921
anaerobic spondylodiscitis: case series and systematic review.bacterial spondylodiscitis is rarely caused by anaerobic organisms. we describe two patients with lumbar vertebral osteomyelitis and discitis caused by anaerobic bacteria, including an unusual occurrence after an endodontic procedure, and review the salient clinical features and outcomes of 31 previously reported cases.200515759942
genotypic characterization of bacteria cultured from duck faeces.to characterize the bacterial composition of mallard duck faeces and determine if novel bacterial species are present that could be utilized as potential indicators of avian faecal contamination.200516033461
influence of denatured and intermediate states of folding on protein aggregation.we simulate the aggregation thermodynamics and kinetics of proteins l and g, each of which self-assembles to the same alpha/beta [corrected] topology through distinct folding mechanisms. we find that the aggregation kinetics of both proteins at an experimentally relevant concentration exhibit both fast and slow aggregation pathways, although a greater proportion of protein g aggregation events are slow relative to those of found for protein l. these kinetic differences are correlated with the am ...200515772307
cervical necrotizing fasciitis: an unusual complication of genuine peritonsillar abscess. 200516048699
bacteriology of chronic maxillary sinusitis associated with nasal polyposis.aspirates from 48 chronically inflamed maxillary sinuses from patients who had nasal polyposis were processed for aerobic and anaerobic bacteria. bacterial growth was present in 46 (96%) specimens. aerobic or facultative bacteria were present in 6 (13%) specimens, anaerobic bacteria alone in 18 (39%), and mixed aerobic and anaerobic bacteria in 22 (48%). there were 110 bacterial isolates (2.4 per specimen). thirty-nine of the isolates were aerobic or facultative organisms (0.85 per specimen). th ...200515888469
protein l-agarose for adsorption of autoantibodies: a potential tool for extracorporeal treatment.this work investigated the adsorption of autoantibodies such as anti-ss-a/ro, anti-ss-b/la, anti-sm, and anti-dsdna on protein l-agarose gel. in order to determine better conditions for igg adsorption on this matrix, some buffer systems were tested. adsorption data were analyzed using the langmuir and langmuir-freundlich isotherm models. the experimental isotherms were best described by the langmuir-freundlich model, which indicated negative and positive cooperativities for binding in the presen ...200515787626
synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein l from peptostreptococcus magnus.rational design and combinatorial chemistry were utilized to search for lead protein l (ppl) mimetics for application as affinity ligands for the purification of antibodies and small fragments, such as fab and scfv, and as potential diagnostic or therapeutic agents. inspection of the key structural features of the complex between ppl and human fab prompted the de novo design and combinatorial synthesis of a 169-membered solid-phase ligand library, which was assessed for binding to human igg and ...200515688433
mechanically unfolding the small, topologically simple protein l.beta-sheet proteins are generally more able to resist mechanical deformation than alpha-helical proteins. experiments measuring the mechanical resistance of beta-sheet proteins extended by their termini led to the hypothesis that parallel, directly hydrogen-bonded terminal beta-strands provide the greatest mechanical strength. here we test this hypothesis by measuring the mechanical properties of protein l, a domain with a topology predicted to be mechanically strong, but with no known mechanica ...200515863479
associations between igg antibody to oral organisms and carotid intima-medial thickness in community-dwelling adults.the aims of this study are to describe the relationships between igg antibodies to 17 oral organisms and atherosclerosis as indexed by carotid intima-medial wall thickness (imt) and to evaluate the role of smoking.200515893320
infected neonatal cephalohematomas caused by anaerobic bacteria.to present the microbiological and clinical features of six children with infected cephalohematomas (ic) caused by anaerobic bacteria.200515914351
[surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2003].for the post-marketing surveillance of cefmetazole (cmz, cefmetazon), mics of injectable beta-lactam antibacterials including cmz against clinical isolates from 15 medical institutions all over japan are measured yearly and the incidence rates of resistance in various species are also evaluated. in the first surveillance from june 2000 to march 2001, 574 isolates of 13 species were tested, 548 isolates of the same 13 species were tested in the second surveillance from april 2001 to march 2002, a ...200516161756
identification and antimicrobial susceptibility of micro-organisms recovered from cutaneous lesions of human american tegumentary leishmaniasis in minas gerais, brazil.an evaluation of the microbiota present in cutaneous ulcers from 31 patients with a clinical and parasitological diagnosis of american tegumentary leishmaniasis (atl) was carried out by the standard filter paper disc technique, including antimicrobial susceptibility of the bacterial isolates. microbial examination indicated that 21 patients (67.7%) were contaminated with one to four bacteria and some of them also with yeast. a total of 142 micro-organisms were isolated. staphylococcus aureus was ...200516192439
identification of cultivable microorganisms from primary endodontic infections with exposed and unexposed pulp space.this study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. the canal contents were analyzed by aerobic/anaerobic culture, and conventional identification techniques. there were 211 isolates of bacteria belonging to 28 genera and 55 species recovered from exposed canals. in the unexpose ...200515917680
comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens.the purpose of this study was to compare the antimicrobial activities of 6% and 3% sodium hypochlorite (naocl), 2% and 0.12% chlorhexidine gluconate (chx), and 0.01% and 0.005% doxycycline (doxy) on four microorganisms associated with primary endodontic infections. the agar diffusion test was used to measure antimicrobial activities of these agents against peptostreptococcus micros, prevotella intermedia, streptococcus sanguis, and lactobacillus acidophilus. minimum inhibitory concentration anal ...200515917691
periodontal pathogens: a quantitative comparison of anaerobic culture and real-time pcr.periodontitis is a multi-factorial chronic inflammatory and destructive disease of the tooth-supporting tissues. quantitative anaerobic culture techniques have been used for microbial diagnosis of the different forms of the disease. the aim of this study was to compare real-time pcr with quantitative anaerobic culture for detection and quantification of 5 prominent periodontal pathogens. real-time pcr assays with the 16s rrna genes of actinobacillus actinomycetemcomitans, prevotella intermedia, ...200515919188
different crystal packing in fab-protein l semi-disordered peptide complex.proteins and peptides with variable degrees of disorder are a challenge for protein crystallization. these may be completely disordered or just contain regions with a high degree of mobility that may be represented by a multitude of discretely defined conformations. these difficulties are not insurmountable, but it may be unreasonable to expect a clean result from a structural point of view. the complex between a murine monoclonal antibody (19d9d6) and a synthetic peptide that encompasses the fi ...200515930632
antimicrobial susceptibility variation of 50 anaerobic periopathogens in aggressive periodontitis: an interindividual variability study.the frequent use of antibiotics in developed countries has led to the emergence of widespread bacterial resistance. in this study, the interindividual variability of the antibiotic susceptibility of 50 putative microorganisms in aggressive periodontitis patients has been evaluated by means of vc (variation coefficient).200515943770
meningioma associated with abscess formation--a case report.a rare case of meningioma associated with both intratumoral and peritumoral abscess formation occurred in a 38-year-old man presenting with signs and symptoms of elevated intracranial pressure, intracranial infection, and right temporal pole mass lesion. the mass lesion was totally removed, revealing a meningioma. group b streptococcus and peptostreptococcus were cultured from both the tumor and peritumoral white matter. hematogenous spread of the organisms related to recent dental work was the ...200515936397
comparison of the crystallization and crystal packing of two fab single-site mutant protein l complexes.protein l from peptostreptococcus magnus (ppl) is a multidomain protein composed of four or five immunoglobulin-binding domains that target the kappa light chain of a large repertoire of human and murine antibodies. thus, a single domain of this protein can be used to aid the crystallization of fab, free or complexed to their antigen when it is not possible to obtain crystals without it. each wild-type ppl domain has two light-chain binding sites that target the same region of the light chain an ...200515930633
recovery of potential pathogens and interfering bacteria in the nasopharynx of smokers and nonsmokers.active smoking and passive exposure to cigarette smoke are associated with colonization by some potentially pathogenic species of bacteria and an increased risk of respiratory tract infection in both adults and children. in an attempt to explain these observations, this study compared the frequency of isolation of potential pathogens, and aerobic and anaerobic bacteria that possess interfering capabilities (ie, interfering with the in vitro growth of potential pathogens) in the nasopharynx of sm ...200515947322
prevalence of periodontopathic bacteria in aggressive periodontitis patients in a chilean population.actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis (agp). other periodontopathic bacteria such as porphyromonas gingivalis are also suspected of participating in aggressive periodontitis although the evidence to support this is controversial. the aim of the present study was to determine the prevalence of eight periodontopathic bacteria in chilean patients with agp.200515974855
[frequency, diagnostic criteria and consequences of bacterial vaginosis in pregnant women].vaginal discharge based on changes in a vaginal ecosystem (decreasing number of lactobacillus and increasing number of mobiluncus spp., gardnerella vaginalis, prevotella spp., mycoplasma hominis, peptostreptococcus spp) appears to be a major cause of bacterial vaginosis. bacterial vaginosis is associated with: preterm delivery, premature rupture of membranes, chorioamnionitis, amniotic fluid infection, endometritis, low-weight neonates. the role of natural microflora of vagina, the most importan ...200415730019
clinical and microbiological associations in chronic periodontitis.chronic periodontitis is the most prevalent form of periodontal disease. the subgingival microflora has been associated with the onset and progression of the disease. the aim of this work was to establish associations and correlations between clinical indicators and subgingival plaque microbiological data in patients diagnosed with moderate or severe periodontitis. one hundred sites with a probing depth of > or = 5 mm were selected. plaque index (pi), gingival index (gi), bleeding on probing (bp ...200415584257
comparative analysis of the four rrna operons in finegoldia magna atcc29328.there are four rrna operons rrna, rrnb, rrnc and rrnd on the genome of finegoldia magna (formerly peptostreptococcus magnus) atcc29328, which, in contrast to those of clostridia, are dispersed around the chromosome. using a bac library we determined the nucleotide sequences and structures of all four operons, including their flanking regions, and performed comparative analyses. we identified putative boxa sequences in the operons, which should be required for rrna transcription antitermination, ...200415053317
septic arthritis of the hip and intrapelvic abscess following intra-articular injection of hylan g-f 20. a case report. 200415069151
[bacterial infections in chronic otitis media--usefulness of molecular diagnostics based on pcr method].infections in chronic otitis media (com) are common, and identification of their pathogen is difficult, what is proven by high rate of negative results of bacteriological examinations. this fact may be explained by two factors: it is difficult to obtain a reliable material for culture and classic methods of bacteriological cultures may be of low sensitivity. amplification of bacterial dna in pcr is an examination, that leads to a higher effectiveness and sensitivity of bacteriological tests. the ...200415311593
periimplantitis.orodental rehabilitation through the use of implants offers very high success rates. in this paper, we describe some of the complications involved with this technique, such as periimplant disease and, within this category, periimplantitis, an inflammatory reaction in which there is a loss of the bony support of the implant accompanied by inflammation. the aetiology of the disease is conditioned by the status of the tissue surrounding the implant, implant design, degree of roughness, the poor ali ...200415580138
socket infection due to retained gauze after evisceration.a 64-year-old man had late-onset socket infection due to retained surgical gauze after evisceration. external examination showed a mass of retained surgical gauze with copious yellowish discharge and gas bubbles. computed tomography scans showed a 3.2 x 2.4 x 2.4-cm heterogeneous mass and numerous gas bubbles. culture of the discharge yielded pseudomonas aeruginosa, streptococcus viridans, peptostreptococcus species, and fusobacterium species. surgical debridement and antibiotic therapy achieved ...200415580975
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