PMID(sorted ascending)
[clinical evaluation of imipenem/cilastatin sodium in the internal medicine].fifty-two patients with moderate or severe infections associated with internal medicine were treated with imipenem/cilastatin sodium (ipm/cs) and the efficacy and the safety of this drug were evaluated. there were 20 patients with pneumonia, 10 with acute exacerbation of chronic respiratory tract infections, 9 with sepsis, 2 with pyothorax, 3 with intraabdominal infection, 2 with urinary tract infection, 1 with pulmonary abscess, 1 with infective endocarditis, 4 with fever of unknown origin. for ...19911920813
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
cos degradation by selected co-utilizing bacteria. scientific note. 19911929384
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...19911940739
specificity of antibodies present in human periapical lesions.various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...19911940740
microbiologic causes and neonatal outcomes associated with chorioamnion infection.chorioamnion infection is associated with histologic chorioamnionitis and prematurity, but the specific chorioamnion microorganisms associated with histologic chorioamnionitis, prematurity, and poor neonatal outcome have not been identified. bacteria were recovered from the chorioamnion cultures of 32% of 112 placentas delivered at less than or equal to 34 weeks' gestation and from 19% of 156 placentas delivered at greater than 34 weeks' gestation (odds ratio 2.1; 95% confidence interval 1.1 to ...19911951562
effects of propicillin on mixed continuous cultures of periodontal bacteria.experiments were designed to test the antibiotic (1-phenoxypropyl)penicillin (propicillin) against a complex microflora of periodontal bacteria. this was accomplished by using a continuously growing mixed culture that was obtained by enrichment of periodontal plaque in human serum. peptostreptococcus species, prevotella intermedia, lactobacillus, catenaforme, and streptococcus species were dominant members of the enrichment culture. none of the strains isolated from the enrichment culture exhibi ...19911952836
the partial amino acid sequence of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum: implications for the evolution and structural basis of coenzyme specificity.the amino acid sequence is reported for cnbr and tryptic peptide fragments of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum. together with the n-terminal sequence, these make up about 75% of the total sequence. the sequence shows extensive similarity with that of the nadp(+)-dependent glutamate dehydrogenase of escherichia coli (52% identical residues out of the 332 compared) allowing confident placing of the peptide fragments within the overall sequence. this demonstrate ...19911954226
[antibacterial activities of ofloxacin against recent isolates from patients with community-acquired infections].in order to survey antibacterial activities of ofloxacin (oflx) against 1,440 bacterial strains isolated from patients with community-acquired infections in 1987 and 1990, minimum inhibitory concentrations (mics) of the drug as well as those of other new quinolones and oral cephems were determined. the following conclusions were reached. 1. comparison of the mic distribution for strains isolated in 1987 with those in 1990 suggested a tendency toward an increase in the frequency of oflx-resistant ...19911960856
comparative efficacy of cefoperazone, cefoperazone plus sulbactam, ciprofloxacin, clindamycin, metronidazole, and penicillin g against anaerobic bacteria in an animal model.treatment efficacy of various antimicrobial regimens against anaerobes was studied in semipermeable chambers simulating a closed-space, locally neutropenic infection site in rabbits. bacteroides fragilis, bacteroides melaninogenicus, clostridium perfringens, and peptostreptococcus anaerobius were inoculated (at a mean of 5.3 log10 cfu/ml in prereduced pooled rabbit serum) into the chambers (one isolate per chamber) in triplicate. antimicrobial therapy consisted of cefoperazone, cefoperazone plus ...19901967626
summer mastitis in heifers: a bacteriological examination of secretions from clinical cases of summer mastitis in denmark.a total of 166 samples of secretions collected from danish heifers suffering from clinically diagnosed summer mastitis were examined bacteriologically. one hundred and sixty three samples yielded positive findings whereas no growth was obtained from 3 specimens. the majority of samples revealed a mixed flora in which the predominant components were actinomyces pyogenes, peptostreptococcus indolicus, a microaerophilic coccus (stuart-schwan coccus), fusobacterium necrophorum, bacteriodes melaninog ...19901973009
[anaerobic bacteria and their role in recurrent tonsillitis].the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ...19901981606
aerobic and anaerobic microbiology of acute suppurative parotitis.aspirates of pus from acute suppurative parotitis were studied for aerobic and anaerobic bacteria. bacterial growth was present in 23 specimens. a total of 36 bacterial isolates (20 anaerobic and 16 aerobic and facultative) were recovered, accounting for 1.6 isolates per specimen (0.9 anaerobic and 0.7 aerobic and facultative). anaerobic bacteria only were present in 10 (43%) patients, aerobic and facultatives in 10 (43%), and mixed aerobic and anaerobic flora in 3 (13%). single bacterial isolat ...19911992268
aerobic and anaerobic microbiology of peritonsillar abscess.thirty-four aspirates of pus from peritonsillar abscesses that were studied for aerobic and anaerobic bacteria showed bacterial growth. a total 107 bacterial isolates (58 anaerobic and 49 aerobic and facultative) were recovered, accounting for 3.1 isolates per specimen (1.7 anaerobic and 1.4 aerobic and facultatives). anaerobic bacteria only were present in 6 (18%) patients, aerobic and facultatives in 2 (6%), and mixed aerobic and anaerobic flora in 26 (76%). single bacterial isolates were reco ...19912000017
microbiological study of hiv-related periodontitis.the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ...19912002434
metabolism of 1,4-dinitro-2-methylpyrrole, a mutagen formed by a sorbic acid-nitrite reaction, by intestinal bacteria.1,4-dinitro-2-methylpyrrole (dnmp), a mutagenic product formed by the interaction of two common food additives, sorbic acid and sodium nitrite, was transformed to 1-nitro-2-methyl-4-aminopyrrole (nmap) by human fecal mixtures and various intestinal bacterial strains. under anaerobic conditions the cell suspensions of actinomyces, bacteroides, clostridium, eubacterium, fusobacterium, and peptostreptococcus spp. demonstrated the nitroreduction activity. under aerobic conditions, only actinomyces a ...19912022195
microbiological studies of decubitus ulcers in children.specimens from 58 children with decubitus ulcers were cultured for aerobic and anaerobic bacteria. aerobic bacteria only were present in 29 (50%) ulcers, anaerobic bacteria only were recovered in 5 (9%), and mixed aerobic and anaerobic flora were present in 24 (41%). a total of 132 isolates (79 aerobes, 53 anaerobes) were recovered, an average of 2.3 isolates per specimen (1.4 aerobes, 0.9 anaerobes). the smallest number of isolates was recovered in ulcers of the skull (1.7 per site), and the hi ...19912023086
toothbrushing and transient bacteremia in patients undergoing orthodontic treatment.this study was made to determine whether and to what extent bacteremia occurred after toothbrushing in patients undergoing orthodontic treatment with fixed appliances. twenty patients were selected, all with negative history of heart or hematologic disorders. these patients had not taken antibiotics or had a history of a cold in the previous 30 days. blood samples of 20 ml were drawn before and 5 minutes after brushing. the immune status of the patients was tested by measurement of isohemaggluti ...19912028936
the laboratory identification of gram-positive anaerobic cocci.a collection of 256 clinical strains and 40 reference strains of gram-positive anaerobic cocci (gpac) was studied, to characterise the recognised species more fully and to define groups of strains which might correspond to previously undescribed species. the methods used were: gas-liquid chromatography (glc) for the detection of volatile fatty acids (vfas); determination of the pre-formed enzyme profile with a commercially available kit, atb 32a; microscopic appearance; colonial morphology; and ...19912030504
oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections.two hundred and seventy bacterial isolates were obtained from the pharyngeal tonsillar surface of 12 normal horses and 98 obligatory anaerobic bacteria were characterised. of these, 57 isolates belonging to 7 genera (peptostreptococcus (1); eubacterium (9); clostridium (6); veillonella (6); megasphera (1); bacteroides (28); fusobacterium (6)) were identified, and 16 of these were identified to species level (p. anaerobius (1); e. fossor (9); c. villosum (1); b. fragilis (1); b. tectum (2); b. he ...19912031304
adhesion of 1-carbon utilizing bacteria to polymeric surfaces. 19912037198
[antibacterial activity of ciprofloxacin against fresh clinical isolates from superficial suppurative foci].the minimum inhibitory concentrations (mics) of 5 drugs (ciprofloxacin (cpfx), and 4 drugs used as standard) were determined to investigate antibacterial potencies of cpfx against bacterial strains isolated in 1989 from superficial suppurative foci. the clinical isolates tested included 375 strains from 11 aerobic bacterial species, and 50 strains from 2 anerobic bacterial genera (group) for a total of 425 isolates. interpreting mic level distributions of these drugs as the expression of antibac ...19912041153
vaginal bacteroides species are associated with an increased rate of preterm delivery among women in preterm labor.the relationship of high concentrations of vaginal microorganisms with an increased rate of preterm delivery (less than or equal to 34 weeks) among women in preterm labor was evaluated. quantitative vaginal cultures were obtained from 211 women in preterm labor between gestational ages of 22 and 34 completed weeks. preterm delivery occurred in 139 (66%). the rate of preterm delivery was determined by cox proportional hazards models. women with greater than 10(7) cfu/ml of vaginal fluid of facult ...19912056221
the differentiation of asaccharolytic anaerobic gram-positive cocci by protein electrophoresis.conventional biochemical and antibiotic sensitivity tests were used to allocate 87 clinical isolates of anaerobic gram-positive cocci to currently recognised species, in comparison with type and other authentic reference strains. whole-cell protein electrophoresis was then performed with extracts of each strain. allowing for difficulties of standardisation, it was possible to allocate most of the organisms to species-related groups on the basis of protein patterns. organisms identified conventio ...19912056518
pyoderma caused by peptostreptococcus tetradius in a pup.pyoderma caused by peptostreptococcus tetradius in a pup was successfully treated with cefadroxil. although this bacterium is rarely found in veterinary medicine, it is a known pathogen in human beings and should be considered as a potential causative agent in pyodermas in which anaerobes are suspected.19912061185
purification and properties of an iron-sulfur-containing and pyridoxal-phosphate-independent l-serine dehydratase from peptostreptococcus asaccharolyticus.l-serine dehydratase with a specific activity of 15 nkat/mg protein was present in the anaerobic eubacterium peptostreptococcus asaccharolyticus grown either on l-glutamate or l-serine. the enzyme was highly specific for l-serine with the lowest km = 0.8 mm ever reported for an l-serine dehydratase. l-threonine (km = 22 mm) was the only other substrate. v/km for l-serine was 500 times higher than that for l-threonine. l-cysteine was the best inhibitor (ki = 0.3 mm, competitive towards l-serine). ...19912065681
chronic prosthetic vascular graft infection visualized with technetium-99m-hexamethylpropyleneamine oxime-labeled leukocytes.technetium-99m-hmpao labeled leukocytes demonstrated chronic femoro-femoral prosthetic vascular graft infection several times during an 18-mo period in a 77-yr-old man. the intensity and distribution of the uptake in the graft were fluctuating in different imaging occasions possibly indicating the strength and location of the infection. gallium-67-citrate imaging showed negative results twice. the reason for negative 67ga results remained obscure. the infected graft was removed and the patient d ...19912066799
polysaccharides from peptostreptococcus anaerobius and structure of the species-specific antigen.the cell-envelope antigens of peptostreptococcus anaerobius were extracted from intact cells by autoclave or alkaline treatment. the purified species-specific antigen (g) was identified among several polysaccharides obtained from the extracts by successive treatments with ribonuclease and pronase followed by ion-exchange and gel-filtration chromatography. g was investigated by 13c- and 31p-n.m.r. spectroscopy, titrimetry, elemental analysis, and gas-liquid chromatography. oxidation of g with nai ...19902076510
the formation of hydrogen sulfide and methyl mercaptan by oral bacteria.the capacity to form volatile sulfur compounds was tested in bacteria isolated from subgingival microbiotas and in a representative number of reference strains. a majority of the 75 tested oral bacterial species and 7 unnamed bacterial taxa formed significant amounts of hydrogen sulfide from l-cysteine. the most active bacteria were found in the genera peptostreptococcus, eubacterium, selenomonas, centipeda, bacteroides and fusobacterium. methyl mercaptan from l-methionine was formed by some mem ...19902082242
localization and identification of root canal bacteria in clinically asymptomatic periapical pathosis.twenty-one teeth with clinically asymptomatic periapical pathosis (class 3) were extracted and the isolation, identification, and localization of bacteria in the root apex were examined. mixtures involving several bacteria were isolated from more than 60% of the cases. scanning electron microscopy revealed bacterial masses to be associated with the apical part of the root canal, but not with the area of apical foramen or on the surface of root apex. our results indicate that the bacteria in clas ...19902084210
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. research group for testing imipenem susceptibility on clinical isolates].we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at 459 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were performed at each hospital laboratory and the tests were carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: , ++, + and -. ipm had significantly high activity against st ...19902086814
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...19902087341
bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment.phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ...19902098335
the effect of metronidazole on the anaerobic microorganisms of the root canal--a clinical study.the antibacterial effect of 0.25% metronidazole solution as root canal irrigant on anaerobic bacteria was studied in ten central incisors. each tooth was treated at four appointments, and the presence of anaerobes in the root canal was studied on each occasion. no antibacterial intracanal dressings were used between the appointments. no anaerobes could be recovered from any tooth on the fifth appointment. in the control group, where normal saline was used as an irrigant, anaerobes could be recov ...19902100667
perforated jejunal diverticulum with multiple hepatic abscesses.we have described a patient with multiple hepatic abscesses caused by a perforated jejunal diverticulum with a presumed route of infection via the portal vein. patients with hepatic abscesses and no known source of infection should be evaluated for a contained mesenteric perforation of the gastrointestinal tract. finally, in patients who fail to respond promptly to percutaneous catheter drainage of a liver abscess, a continuing source of infection, such as perforation of a jejunal diverticulum, ...19902105531
[a clinical study of asymptomatic bacteriospermia].asymptomatic bacteriospermia was studied as an entity and its treatment was investigated in 65 subjects who clinically needed their semen tested. the obtained results are summarized as follows. 1. the rate of detection of bacteria in semen was 89.2%, and the rates of detection of aerobic and anaerobic bacteria were 55.4% and 70.8%, respectively. forty gram-positive strains were identified among the aerobic bacteria; rates of detection for staphylococcus epidermidis and enterococcus faecalis were ...19902112205
intra-abdominal abscess in children: a 13-year experience.the microbiology of intra-abdominal abscess in children is similar to that of adults--a mixture of aerobes and anaerobes. in abscess specimens taken from 36 children, predominant pathogens were bacteroides fragilis group species, peptostreptococcus species, and escherichia coli.19902120268
suppurative oral candidosis. review of the literature and report of a case.a case of a dento-alveolar abscess due to a polymicrobial infection with a yeast, candida guilliermondii and two other oral bacteria are described. a review of the literature has revealed only a single case of an odontogenic abscess due to candida species and we believe this to be the first report of a dentoalveolar infection where candida guilliermondii is associated as a co-pathogen.19902124592
a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ...19902131168
[a microbiologic study on anaerobic bacteremia after dental extraction].it has been known for many years that tooth extraction can cause bacterial dissemination into blood stream, that is, bacteremia. besides endocarditis--causing bacteria, alpha-hemolytic streptococci, anaerobes are involved in the post- extraction bacteremia. they are of clinical importance because they can cause abscesses in the various parenchymatous organs such as the liver, lung, and brain through anachoresis. the purpose of this study is to establish the types of species of anaerobes associat ...19902133345
[morpho-functional characteristics of bacterial species of major importance in endodontic infections].most of the bacteria involved in the endodontic infection can be considered as "highly specific", for they appear to be almost irrelevant in the large field of the other human disease from infection. namely the most important anaerobia responsible for endodontic infection are: bacteroides, fusobacterium, peptostreptococcus, and veillonella. the group of bacteroides is composed by several species with different metabolic and genetic characteristics. they are gram-, non-motile, non-spore-forming r ...19902133812
[significance of the bacterial flora in the etiology of apical periodontitis. qualitative, quantitative and topographical aspects].in the international literature it is shown the central role of root canal infection in the etiology of periapical lesions. as a matter of fact it has been proved (13) that sterile necrotic pulp tissue is completely unable to cause inflammatory reactions at the periapex. infection of endodontic origin extends to the supporting tissues of the tooth only in the case of their acute inflammation (e.g. acute apical periodontitis, acute alveolar abscess, phoenix abscess). on the other hand in chronic ...19902135040
in vitro activity of teicoplanin, vancomycin, a16686, clindamycin, erythromycin and fusidic acid against anaerobic bacteria.the in vitro activity of teicoplanin and a16686, two new glycopeptide antibiotics was determined against 196 isolates of anaerobic bacteria. the activity of teicoplanin and a16686, in comparison with that of vancomycin, clindamycin, erythromycin and fusidic acid was 2 to 16 times higher against the gram positive anaerobes, namely, propionibacterium acnes, clostridium perfringens, clostridium difficile, clostridium species, peptococcus species and peptostreptococcus species. however, bacteroides ...19902139737
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
[improving the clinico-microbiological diagnosis and treatment of non-clostridial anaerobic peritonitis].on the basis of clinico-microbiological examinations the presence of anaerobic flora was found in 104 out of 198 patients with peritonitis. the authors propose using intra-aortal++ transfusions of oxygenated blood in complex therapy of anaerobic peritonitis.19902175490
transferable tet m in fusobacterium nucleatum.two tetracycline-resistant fusobacterium nucleatum strains were able to transfer, by conjugation, tetracycline resistance and the tet m determinant to recipient f. nucleatum, peptostreptococcus anaerobius, and enterococcus faecalis strains at a frequency of 10(-1) to 10(-8).19902178339
cerebral ventriculitis: mr findings.cerebral ventriculitis is an uncommon site and manifestation of infection of the central nervous system. we report the magnetic resonance findings in two patients with cytologic and csf culture proof of ventriculitis. the findings included abnormal signal intensity of the ependyma and csf on t2-weighted images. in addition, ependymal, meningeal, and dural enhancement was seen in one case, on t1-weighted images, after administration of gd-diethylenetriamine pentaacetic acid.19902179305
etiology and treatment of post-cesarean-section endometritis after cephalosporin describe the microbiologic etiology of post-cesarean endometritis developing after perioperative cephalosporin prophylaxis, endometrial samples were obtained from 27 women with a triple-lumen catheter. the women were assigned in a double-blind, randomized fashion to receive either ticarcillin/clavulanate, 3.1 g, or cefoxitin, 2 g, administered every six hours, until the clinical signs of infection resolved. a total of 149 microorganisms (84 facultative and 65 obligate anaerobes) were recovere ...19902181119
parietal lobe abscess after routine periodontal recall therapy. report of a case.this paper reports on a case of a 70-year-old physician diagnosed with a parietal lobe abscess following such treatment. after stereotactic biopsy and drainage and a 6-week course of intravenous antibiotic treatment, the patient recovered with minimal neurologic deficits. although brain abscesses are not commonly encountered in practice, clinicians must be aware of the potential virulence of the anaerobic components of the periodontal pocket and the possibility of resulting systemic infection, w ...19902182824
optimum therapy for acute pelvic inflammatory disease.neisseria gonorrhoeae is responsible for about one-third to one-half of cases of acute pelvic inflammatory disease (pid), although there is considerable geographical variation. chlamydia trachomatis is also an important aetiological agent, and is currently isolated 4 times more commonly from the cervix than the gonococcus. however, it is now clear that acute pid is polymicrobial in aetiology. even when n. gonorrhoeae and/or c. trachomatis are isolated from the endocervix, anaerobes such as bacte ...19902190794
susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations.a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ...19902202809
purification and properties of a nadh-dependent 5,10-methylenetetrahydrofolate reductase from peptostreptococcus productus.the methylenetetrahydrofolate reductase from the carbon-monoxide-utilizing homoacetogen peptostreptococcus productus (strain marburg) has been purified to apparent homogeneity. the purified enzyme catalyzed the oxidation of nadh with methylenetetrahydrofolate as the electron acceptor at a specific activity of 380 mumols.min-1 mg protein-1 (37 degrees c; ph 5.5). the apparent km for nadh was near 10 microm. the apparent molecular mass of the enzyme was determined by gel filtration to be approxima ...19902209595
[a case of cervico-mediastinal abscess, secondary to acute tonsillitis: investigation of the treatments].no other infectious diseases in the field of otolaryngology cause rapid and lethal course than cervical abscess. a case of cervicomediastinal abscess secondary to acute tonsillitis was presented. the patient was a 43-year-old male with liver cirrhosis and primarily had the treatment of tonsillitis. the complication of duodenal perforation caused marked general deteriotation, and cervical abscess occured. immediately after transfer to our department, he was treated by cervical drainage, laparotom ...19902213351
recolonization of the subgingival microflora after scaling and root planing in human periodontitis.the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ...19902213468
septic shock complicating drainage of a bartholin gland abscess. 19902216255
formation of amphetamine from its nitro analogue by anaerobic intestinal bacteria.1. microbial reduction of 1-phenyl-2-nitropropane 1 was carried out using 40 strains of intestinal anaerobic bacteria. among them, 12 strains (mitsuokella multiacidus, clostridium perfringens, c. innocuum, c. clostiriiforme, c. difficile, c. butyricum, c. sp., eubacterium limosum, e. aerofaciens, e. multiforme, peptostreptococcus anaerobius and p. productus) had the ability to reduce 1 to amphetamine 2 (0.1-1% yield). 2. clostridium species were more active than another intestinal anaerobic bact ...19902219956
use of modified norleucine-tyrosine broth in identification of peptostreptococcus anaerobius.gas-liquid chromatography was employed to analyze the volatile and nonvolatile acids produced in modified norleucine-tyrosine (mnt) broth by various gram-positive cocci. the mnt broth consists of 0.5% trypticase (bbl microbiology systems, cockeysville, md.), 0.5% yeast extract (difco laboratories, detroit, mich.), 0.2% l-norleucine, and 0.1% l-tyrosine. the microorganisms included reference strains and clinical isolates of peptostreptococcus spp. (p. anaerobius, p. asaccharolyticus, p. indolicus ...19902229394
impending rupture of nonaneurysmal bacterial aortitis: ct diagnosis.the ct appearance of mycotic abdominal aortic aneurysms leading to eventual rupture has been well described. on the other hand, ruptured nonaneurysmal bacterial aortitis has not been demonstrated in the ct literature. we present two cases with subsequent rupture documented on ct. the characteristic findings of periaortic density and adjacent gas collection should suggest the diagnosis of acute aortic infection. this may herald impending rupture, even in the absence of aneurysmal dilatation, and ...19902229573
[a clinical study on ceftazidime in the treatment of intractable respiratory infections].ceftazidime (caz) was administered to 51 patients (37 males, 14 females) with respiratory infections including severe cases, accompanied by various underlying respiratory diseases. the clinical efficacy and side effects of caz were investigated. the mean age and body weight of these 51 cases were 62.6 years and 48.0 kg, respectively. caz was administered by intravenous drip infusion (daily dose of 2-4 g) for a mean of 14.7 days to a mean total dose of 56.7 g. clinical efficacy rates were 64% (18 ...19902232147
isolation and identification of anaerobic bacteria from ovine foot rot in spain.a microbiological study was made of 125 merino sheep showing clinical signs of foot rot. a total of 435 strictly anaerobic strains were isolated, belonging to the following genera: bacteroides, peptostreptococcus, tissierella, fusobacterium, megasphaera, eubacterium, acidaminococcus, clostridium, peptococcus and propionibacterium. of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: bacteroides nodosus, b putredinis, b buccae, b ruminicola subspecies ...19902236925
aerobic and anaerobic bacteriology of wounds and cutaneous abscesses.the aerobic and anaerobic microbiologic characteristics of 584 wounds and 676 skin or soft-tissue abscesses were studied and correlated with the infection site. in wounds, aerobic or facultative bacteria only were present in 223 specimens (38%), anaerobes only in 177 specimens (30%), and mixed flora in 184 specimens (32%). in total there were 1470 isolates, 558 aerobic and 912 anaerobic, an average of 2.5 isolates per wound (1.6 anaerobic and 0.9 aerobic isolates). in abscesses, aerobic or facul ...19902241555
the biotin-dependent sodium ion pump glutaconyl-coa decarboxylase from fusobacterium nucleatum (subsp. nucleatum). comparison with the glutaconyl-coa decarboxylases from gram-positive bacteria.membrane preparations of fusobacterium nucleatum grown on glutamate contain glutaconyl-coa decarboxylase at a high specific activity (13.8 nkat/mg protein). the enzyme was solubilized with 2% triton x-100 in 0.5 m nacl and purified 63-fold to a specific activity of 870 nkat/mg by affinity chromatography on monomeric avidin-sepharose. the activity of the decarboxylase was strictly dependent on na+ (km = 3 mm) and was stimulated up to 3-fold by phospholipids. the glutaconyl-coa decarboxylases from ...19902244788
[asporogenic anaerobic empyema--clinical and bacteriological investigations of 31 patients with anaerobic empyema].the author reviewed the records of 31 patients with asporogenic anaerobic empyema mostly seen in the wards of internal medicine, juntendo university hospital during the 27 years between 1961 and 1988, and obtained the following results. 1. there were 25 males and 8 females with an average age of 57.8 and 51.0 y/o (range, 25 to 79 y/o), respectively males more than forty years old occupied 74.2 percent of all cases. 28 patients (90%) had underlying conditions. 2. the cases of mixed infections wit ...19902258645
[mitral valve prolapse in infective endocarditis. incidence and characteristics].purpose valve prolapse (mvp) cases, complicated with infective endocarditis (ie), seen at a cardiologic reference hospital.19902260932
[effect of helium-neon-laser radiation on microbes].in a well-defined in-vitro experiment the effects of a he-ne-laser of 6 mw output on some dominating microbes in the oral cavity were studied by using a scanning electron microscope. no changes on the surface in the irradiated colonies could be seen even after an exposition lasting for 60 minutes (continuous or pulsated wave). therefore an antibacterial effect of softlaser-irradiation sometimes described in literature in questionable.19902270558
obligately anaerobic bacterial species isolated from foot-rot lesions in goats.lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ...19902271912
antibody response in immunized rabbits measured with bacterial immunoglobulin-binding proteins.protein g, an immunoglobulin (ig)-binding protein isolated from group c or g streptococci, binds to the fc portion of igg. protein l, from the anaerobic bacterium peptostreptococcus magnus, specifically binds light chains of ig. in this study, protein g and l were used to measure the production of antibodies in immunized rabbits. two rabbits were immunized with a mixture of human urinary proteins from a patient with tubular proteinuria, and blood samples were collected regularly from the animals ...19902273256
cephalexin concentrations in human serum, gingiva, and mandibular bone following a single oral administration.1. cephalexin concentrations in human serum, gingiva, and mandibular bone after a single oral administration of cephalexin (500 mg) were measured by the paper disc method. 2. the peak times of serum, gingiva, and mandibular bone were approximately 90, 120 and 120 min, respectively. 3. the peak concentrations of serum, gingiva, and mandibular bone were 10.58 micrograms/ml, 5.57 micrograms/g and 2.12 micrograms/g, respectively. 4. the concentration ratio of gingiva/serum and mandibular bone/serum ...19902276582
[anaerobic asporogenous infection in patients with postoperative empyema].the results of treatment of 38 patients with postoperative pleural empyema caused by anaerobic asporogenous infection were summarized. the factors contributing to the development of anaerobic asporogenous infection, peculiarities of the clinical picture, diagnosis and treatment were revealed, the measures for prophylaxis were outlined. the clinical effectiveness was achieved in 92.1% of the patients with lethality of 2.6%.19902280516
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates].this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ...19902287060
purification and comparative studies of dihydrolipoamide dehydrogenases from the anaerobic, glycine-utilizing bacteria peptostreptococcus glycinophilus, clostridium cylindrosporum, and clostridium sporogenes.three different dihydrolipoamide dehydrogenases were purified to homogenity from the anaerobic glycine-utilizing bacteria clostridium cylindrosporum, clostridium sporogenes, and peptostreptococcus glycinophilus, and their basic properties were determined. the enzyme isolated from p. glycinophilus showed the properties typical of dihydrolipoamide dehydrogenases: it was a dimer with a subunit molecular mass of 53,000 and contained 1 mol of flavin adenine dinucleotide and 2 redox-active sulfhydryl ...19902294086
late infection after total hip replacement, caused by an oral organism after dental manipulation. a case report. 19902295660
bacterial vaginosis as a risk factor for post-cesarean endometritis.bacterial species associated with bacterial vaginosis have been isolated more frequently from endometrial cultures of patients with postpartum endometritis than expected from the prevalence of bacterial vaginosis among pregnant women. to further assess the association between bacterial vaginosis and postpartum endometritis, vaginal gram smears were obtained from women admitted for delivery. vaginal smears of women delivered by cesarean were scored as normal or as indicating bacterial vaginosis. ...19902296423
anaerobic bacteria and bacterial infections: perspectives on treatment and resistance in italy.results of laboratory tests of 2,000 samples obtained from 1984 to 1987 from patients with suspected anaerobic infections and the clinical experience of these patients are reported. of these samples, 395 were positive for anaerobes; 36.5% of these 395 samples contained single organisms, and 63.5% contained a mixture of anaerobes and aerobes. abdominal infections were the infections most frequently caused by anaerobes. the bacteroides fragilis group and strains of peptostreptococcus were the micr ...19902305181
splenic abscess due to peptostreptococcus spp. as a complication of cardiac catheterization.we describe the development of a splenic abscess, due in part to peptostreptococcus, as a complication of cardiac catheterization. the etiologies of splenic abscess should include peptostreptococcus, and splenic abscesses should be added to the list of complications of cardiac catheterization.19902317856
purification of nadph-dependent electron-transferring flavoproteins and n-terminal protein sequence data of dihydrolipoamide dehydrogenases from anaerobic, glycine-utilizing bacteria.three electron-transferring flavoproteins were purified to homogeneity from anaerobic, amino acid-utilizing bacteria (bacterium w6, clostridium sporogenes, and clostridium sticklandii), characterized, and compared with the dihydrolipoamide dehydrogenase of eubacterium acidaminophilum. all the proteins were found to be dimers consisting of two identical subunits with a subunit mr of about 35,000 and to contain about 1 mol of flavin adenine dinucleotide per subunit. spectra of the oxidized protein ...19902318809
relationship of bacteriologic characteristics to semen indices in men attending an infertility clinic.bacteria can be isolated from most seminal fluid samples, but the significance of these microorganisms is uncertain because most men lack symptoms associated with bacterial infection of the reproductive tract. we obtained semen samples from 37 men attending a special infertility clinic and assessed the relationship between seminal fluid microorganisms and seminal fluid analysis including sperm motility, morphology, and concentration; the numbers of polymorphonuclear leukocytes and other white bl ...19902325963
genetic basis of tetracycline resistance in urogenital bacteria.the distributions of the nucleotide sequences related to the tetracycline resistance determinants tet k, tet l, tet m, and tet o were studied by dot blot hybridization with randomly chosen clinical urogenital tract isolates of viridans group streptococci, streptococcus agalactiae, enterococcus faecalis, gardnerella vaginalis, lactobacillus spp., fusobacterium nucleatum, peptostreptococcus spp., and veillonella parvula. among the peptostreptococcus spp., 79% of the isolates hybridized with one (6 ...19902327774
biotransformations of aromatic aldehydes by acetogenic bacteria.vanillin was subject to o demethylation and supported growth of clostridium formicoaceticum and clostridium thermoaceticum. vanillin was also stimulatory to the co-dependent growth of peptostreptococcus productus. the aldehyde substituent of vanillin was metabolized by routes which were dependent upon both the acetogen and a co-metabolizable substrate (e.g. carbon monoxide [co]). c. formicoaceticum and c. thermoaceticum oxidized the aldehyde group of vanillin to the carboxyl level, while p. prod ...19902328911
[the immunological diagnosis of peptostreptococcal infection].the work deals with different methods for the diagnosis of anaerobic streptococcal infection, experimentally tested and clinically approved in the examination of children with acute pneumonia. the passive hemagglutination test, the immune rosette formation test and the specific lymphocyte blastogenesis test were used for diagnosis. as sensitin and antigen, the preparation of the peptostreptococcal bacterial mass was used. the diagnostic titer of antibodies to anaerobic streptococci was 1:160. a ...19902330780
clinical, pathologic, and microbiologic findings of foot abscess in neonatal pigs.a longitudinal study was undertaken in a swine herd with an ever-present problem of foot abscess in suckling pigs reared on a woven-wire floor. of 3,322 4-day-old pigs, 199 (6%) developed abscess lesions involving claws and accessory digits before weaning. lesions were first detected in 4-day-old pigs; median and mean ages at onset were 10 and 11.3 days, respectively. at first detection, most pigs had only a single claw affected, but 39 pigs had at least 2 claws with abscesses. hind limbs had mo ...19902351598
the obligate and facultatively anaerobic bacterial flora of the normal feline gingival margin.samples from the gingival margins of 14 cats considered normal on clinical examination were cultured for facultative and obligate anaerobic bacteria. all mouths were free from any gingival marginal inflammation and tartar build-up; all cats were between 6 and 12 months of age. a mixed growth was obtained from all samples. the mean number of bacterial species per sample was 10.7 with a range of 7-16 isolates. of the 150 isolates processed, 109 (72.66%) were obligate anaerobes. of the facultativel ...19902353448
the microbiology of chronic sinus disease in children with respiratory allergy.chronic maxillary sinusitis is common in children with respiratory allergy and is associated with increased morbidity. the bacteriology of chronic sinus disease in these children has not been adequately evaluated. between may 1987 and january 1988, 12 children (aged 3 to 9 years), all with documented respiratory allergy and chronic respiratory symptoms consistent with chronic sinusitis (greater than 30 days), were fully evaluated. history, physical examination, complete blood count, nasal smear, ...19902355153
clindamycin versus ampicillin in the treatment of odontogenic infections.the subjects were 106 patients, aged 14 to 70 years, with acute facial or oral abscesses originating from an odontogenic source. they were randomly assigned to receive 150 mg of clindamycin or 250 mg of ampicillin orally four times daily for seven days. after seven days of treatment, the infections were eradicated in 36 of the 52 clindamycin-treated patients and in 42 of the 54 ampicillin-treated patients, and improved in 16 and 11, respectively. one of the ampicillin group was a treatment failu ...19902379229
microbiologic efficacy of intravaginal clindamycin cream for the treatment of bacterial vaginosis.bacterial vaginosis is characterized by replacement of the normal lactobacillus-predominant vaginal flora with gardnerella vaginalis, anaerobic bacteria, and mycoplasma hominis. the present study evaluated the vaginal flora of women with bacterial vaginosis before and after treatment with intravaginal clindamycin cream. sixty-seven nonpregnant women with symptoms and signs of bacterial vaginosis, and without other genital tract infections, were randomly assigned to receive placebo cream or 0.1, ...19902381617
biliary excretion of glutathione conjugates of 4,5-epoxy-4,5-dihydro-1- nitropyrene and 9,10-epoxy-9,10-dihydro-1-nitropyrene in rats administered 1-nitropyrene orally and their further metabolism in the intestinal tract.1-nitropyrene (1-np), a mutagenic and carcinogenic substance that occurs in the environment, is metabolized by reductive and oxidative pathways. 4,5-epoxy,4,5-dihydro-1-np (1-np 4,5-oxide) and 9,10-epoxy-9,10-dihydro-1-np (1-np 9,10-oxide) are oxidatively activated metabolites of 1-np, and react with glutathione. hplc analysis of biliary metabolites from rats administered [3h]1-np orally showed the presence of glutathione conjugates of 1-np 4,5-oxide and 1-np 9,10-oxide and their metabolites, cy ...19902387025
the microbial flora from root canals and periodontal pockets of non-vital teeth associated with advanced periodontitis.microflora from root canals and periodontal pockets of periodontally affected teeth were compared in order to elucidate the as yet unknown relationship between pulpal and periodontal disease. caries-free teeth affected with advanced periodontitis and diagnosed as clinically dead by electric pulp testing were selected. the root canals and periodontal pockets were sampled, and the bacterial flora examined by both culture and interference microscopy. the results indicated that the aerobe/anaerobe r ...19902391182
peptostreptococcus hydrogenalis sp. nov. from human fecal and vaginal flora.we describe peptostreptococcus hydrogenalis sp. nov., which is found in human feces and vaginal discharge. this new species was established on the basis of the results of dna-dna hybridization among anaerobic cocci. the results of different biochemical reactions also are given. the type strain of this species is strain gifu 7662 (= jcm 7635).19902397199
management of empyema thoracis.over a 5-year period, 65 adult patients with empyema thoracis were treated. the cause of the empyema was postpneumonic in 52%, postresectional in 24%, a complication of minor surgical procedures in 14%, posttraumatic in 5%, and the result of miscellaneous causes in 5%. in the postpneumonic group, infection resulted from a single aerobic organism in 41%, multiple aerobic organisms in 9%, single anaerobic organisms in 12%, and mixed aerobic-anaerobic organisms in 18%. peptostreptococcus, streptoco ...19902400254
bacterial vaginosis and trichomoniasis vaginitis are risk factors for cuff cellulitis after abdominal assess the relationship between either bacterial vaginosis or trichomoniasis vaginitis and posthysterectomy infection, preoperative evaluation of the vaginal secretions was performed in 161 women undergoing abdominal hysterectomy. thirty-two patients (19.9%) and 27 patients (16.8%), respectively, met the diagnostic criteria for bacterial vaginosis and trichomoniasis vaginitis. patients with either bacterial vaginosis or trichomoniasis vaginitis were more likely than control subjects to have c ...19902403128
the bacteriology of gangrenous and perforated using optimum sampling, transport, and culture techniques in patients with gangrenous or perforated appendicitis, we recovered than has previously been reported. thirty patients older than 12 years with histologically documented gangrenous or perforated appendicitis had peritoneal fluid, appendiceal tissue, and abscess contents (if present) cultured. appendiceal tissue was obtained so as to exclude the lumen. a total of 223 anaerobes and 82 aerobic or faculatative bacteria were recovered, an ...19902405791
current taxonomy of medically important nonsporing anaerobes.this review deals mainly with the taxonomy of the genera and species in the family bacteroidaceae. it has been proposed that the genus bacteroides should be restricted to include the "bacteroides fragilis group" and that the asaccharolytic black-pigmented bacteroides species be transferred to a new genus, porphyromonas. new bacteroides, fusobacterium, and selenomonas species have been described. a high degree of heterogeneity is apparently present among peptostreptococcus species. mobiluncus is ...19902406865
protein l: an immunoglobulin light chain-binding bacterial protein. characterization of binding and physicochemical properties.protein l, a cell wall molecule of the bacterial species peptostreptococcus magnus with affinity for immunoglobulin (ig) light chains, was isolated after solubilization of the bacterial cell walls with mutanolysin or from the culture medium by a single affinity chromatography step on human igg-sepharose. a major protein band with an apparent molecular weight of 95,000 was obtained from both sources. the protein from the growth medium was size heterogeneous. from 1 ml of packed bacteria was prepa ...19892479638
application of chemotaxonomic techniques to the taxonomy of anaerobic bacteria.the use of chemical characters in bacterial classification and identification has proved to be an essential component of modern systematics. several clinically important anaerobic genera, such as bacteroides, clostridium, eubacterium, fusobacterium and peptostreptococcus, are known to be heterogeneous of the basis of chemotaxonomic and genetic data and are in need of further examination. recent work on bacteriodes has led to the genus being redefined and restricted to species within the former b ...19892479974
[isolation of anaerobes during a 30-month observation at a hospital microbiology laboratory].the authors evaluate retrospectively the results obtained from the research of anaerobial bacteria on 1313 samples received at the microbiology laboratory of the "ospedale civile di ivrea" over a period of 31 months (6/1/86-12/31/88). from this evaluation, high percentages of detection of anaerobic bacteria are emerging in the following infections: appendiculare abscesses (60%), intestinal operations (71%), wounds (57%), tubovarian abscesses (100%), as well as thoracic empyema (50%). also releva ...19892490397
gaspak plus versus anaerocult a--two carbon dioxide/hydrogen systems for cultivation of anaerobes.two disposable carbon dioxide/hydrogen gas-generating systems (gaspak plus and anaerocult a) were compared by assessing growth of obligate anaerobic bacteria. eighty strains representing 28 species of anaerobic bacteria commonly occurring at various body sites were seeded onto 4 brain heart chocolate agar plates using a spiral plater; and 1 plate each was subsequently incubated in 2 anaerocult a and 2 gaspak plus systems. bacterial growth was expressed as colony-forming units per ml (cfu/ml), re ...19892496538
sodium dependent acetate formation from co2 in peptostreptococcus products (strain marburg).washed cells of peptostreptococcus products (strain marburg), which were incubated in the presence of co/co2/n2 (50%/17%/33%; 200 kpa) catalyzed the synthesis of acetate from carbon monoxide. the rate of acetate formation from co was stimulated more than threefold by the addition of sodium (10 mm); potassium did not effect acetate synthesis. the degree of stimulation was dependent on the sodium concentration; the dependence followed simple michaelis-menten kinetics. the apparent km for sodium wa ...19892498155
coumamidines, new broad spectrum antibiotics of the cinodine type. iii. microbiologic activity of coumamidine gamma 1.the coumamidines are novel antibiotics with activity against a wide spectrum of aerobic gram-positive and gram-negative bacteria. all microbiological studies were performed on coumamidine gamma 1. the mic90s (micrograms/ml) of coumamidine are as follows: staphylococcus aureus 1.0, streptococcus pyogenes 8, enterobacteriaceae 2.0, pseudomonas aeruginosa 8, campylobacter jejuni and campylobacter coli 1, legionella pneumophila 8, haemophilus influenzae 0.5, neisseria gonorrhoeae 0.5. coumamidine ha ...19892498268
the active species of "co2" formed by carbon monoxide dehydrogenase from peptostreptococcus productus.carbon monoxide dehydrogenase of anaerobic bacteria catalyzes the reversible conversion of co to "co2". with the enzyme from peptostreptococcus productus it is shown that co2 rather than hco3- (h2co3) is the active species of "co2" formed by this dehydrogenase.19892504176
bacteriotherapy for chronic relapsing clostridium difficile diarrhoea in six patients.six patients with chronic relapsing diarrhoea caused by clostridium difficile were treated with rectal instillation of homologous faeces (one patient) or a mixture of ten different facultatively aerobic and anaerobic bacteria diluted in sterile saline (five patients). the mixture led to a prompt loss of cl difficile and its toxin from the stools and to bowel colonisation by bacteroides sp, which had not been present in pre-treatment stool samples. strains of escherichia coli, cl bifermentans, an ...19892566734
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