PMID(sorted descending)
[microbiological and biochemical characteristics of inflammatory tissues in the periodontium].to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva.200818413987
ocular infections due to anaerobic bacteria in children.the recent increased recovery of anaerobic bacteria from children has led to greater appreciation of the role these organisms play in pediatric infections at all body sites, including the eye. in studies that employed adequate methods for recovery of anaerobes, they were isolated from approximately one-third of the children with conjunctivitis, half of the time in pure culture. the predominant recovered anaerobes were clostridium spp., gram-negative anaerobic bacilli, and peptostreptococcus spp. ...200818404954
microbiology and management of joint and bone infections due to anaerobic describes the microbiology, diagnosis, and management of septic arthritis and osteomyelitis due to anaerobic bacteria.200818392922
clinical and microbiological determinants of ailing dental implants.the failure of the host tissue to establish or maintain osseointegration around dental implants is due to either occlusal or parafunctional forces, premature loading, ill-directed stress, or microbial infection. the long-term failure rate of dental implants is generally 5-10%. although a variety of etiologies of early peri-implant bone loss (from implant placement to 1-year post-loading) have been proposed, factors associated with late implant failures are less well understood but are probably r ...200918384407
a retrospective analysis of isolates from patients with vaginitis in a private greek obstetric/gynecological hospital (2003-2006).vaginitis is a common cause of complaints in women in various parts of the world. through our literature search, we identified noteworthy differences in the etiology of vaginitis based on the geographic location. this study was conducted to describe microbial etiology in a population of symptomatic women with vaginitis in greece.200818376352
[lumbar pyomyositis caused by finegoldia magna (peptostreptococcus magnus) in a patient with hepatitis c virus infection]. 200818373916
moraxella catarrhalis-dependent tonsillar b cell activation does not lead to apoptosis but to vigorous proliferation resulting in nonspecific igm production.the respiratory pathogen moraxella catarrhalis has a high affinity for human igd and is mitogenic for peripheral blood b lymphocytes. moraxella igd-binding protein, which is a multifunctional outer membrane protein with adhesive properties, is responsible for the interaction. previous experiments with the ig-binding b cell superantigens protein a and protein l from staphylococcus aureus and peptostreptococcus magnus, respectively, have suggested that nonimmune bcr cross-linking induces b cell ap ...200818372337
urea and guanidinium chloride denature protein l in different ways in molecular dynamics performing protein-denaturation experiments, it is common to employ different kinds of denaturants interchangeably. we make use of molecular dynamics simulations of protein l in water, in urea, and in guanidinium chloride (gdmcl) to ascertain if there are any structural differences in the associated unfolding processes. the simulation of proteins in solutions of gdmcl is complicated by the large number of charges involved, making it difficult to set up a realistic force field. furthermore, at ...200818339753
anaerobic liver abscesses as initial presentation of silent colonic cancer.rarely, multiple liver abscesses caused by anaerobic organisms are the presenting form of unsuspected colonic cancer in the absence of liver metastases.200418333045
[relationship between clostridium difficile associated diarrhea and intestinal microecosystem disorder in patients received allogeneic hematopoietic stem cell transplantation].this study was to investigate the relationship between clostridium difficile associated diarrhea (cdad) and intestinal microecosystem in patients received allogeneic hematopoietic stem cell transplantation (allo-hsct) and to clarify clinical characteristics of intestinal microecosystem disorder. clostridium difficile (cd) was isolated and identified by enzyme-linked-immunosorbent assay using clostridium difficile premier toxins a&b kit and anaerobic culture in 44 cases with diarrhea. fecal flora ...200818315917
isolation of obligate anaerobic bacteria from ulcerative keratitis in domestic determine the frequency of obligate anaerobic bacterial isolation from corneal samples of domestic animals with ulcerative keratitis and to characterize the historical, clinical, cytological, and microbiological features of culture-positive cases.200818302576
microbiology and management of abdominal infections.this review describes the microbiology, diagnosis, and management of intra-abdominal infections. these infections include secondary peritonitis, abdominal abscesses, and cholangitis. the infection generally occurs because enteric microorganisms enter the peritoneal cavity through a defect in the wall of the intestine or other viscus as a result of obstruction, infarction, or direct trauma. mixed aerobic and anaerobic flora can be recovered. the predominant aerobic isolates are escherichia coli, ...200818288616
smap29 congeners demonstrate activity against oral bacteria and reduced toxicity against oral keratinocytes.cathelicidins are antimicrobial peptides found in epithelial and mucosal tissues as well as the secondary granules of neutrophils. smap29, a sheep cathelicidin, has differential antimicrobial properties against various pathogens, including periodontal organisms. the purpose of this study was to evaluate the antimicrobial properties and cytotoxicity of smap29, smap28, and three congeners (smap18a, smap18d, and smap14a).200818279175
complete genome sequence of finegoldia magna, an anaerobic opportunistic pathogen.finegoldia magna (formerly peptostreptococcus magnus), a member of the gram-positive anaerobic cocci (gpac), is a commensal bacterium colonizing human skin and mucous membranes. moreover, it is also recognized as an opportunistic pathogen responsible for various infectious diseases. here, we report the complete genome sequence of f. magna atcc 29328. the genome consists of a 1,797,577 bp circular chromosome and an 189,163 bp plasmid (ppep1). the metabolic maps constructed based on the genome inf ...200818263572
characterization of chorioamnionitis in 2nd-trimester c-section placentas and correlation with microorganism recovery from subamniotic tissues.prolonged exposure to infection appears to influence fetal/neonatal development. we characterize the relationship between histologic patterns of inflammation and microorganism recovery from the placentas of live born infants delivered before the 28th postmenstrual week. the subamniotic parenchyma of 835 placentas delivered by cesarean section were cultured and evaluated for specific histologic patterns of inflammation in a blinded fashion. cases with prolonged membrane rupture were excluded. mic ...200818237241
anaerobic bacterial pericardial effusion in a cat.a 9-year-old male cat was presented for evaluation of chronic weight loss and was subsequently diagnosed with pericardial effusion. the effusion was quantified as a septic exudate caused by the anaerobic bacterium peptostreptococcus. antibiotic therapy resulted in complete resolution of the pericardial effusion. as peptostreptococcus is a common oral bacterium and the cat had a previous dental procedure, it is speculated that the pericardial effusion was secondary to bacteraemia from the dental ...200718237044
descending necrotizing mediastinitis secondary to retropharyngeal abscess without cervical spread.cervical infections can cause neck phlegmonosa and occasionally develop lethal mediastinitis. we report a 52-year-old man with a retropharyngeal abscess causing descending mediastinis without cervical spread. thoracoscopic drainage without cervicotomy was successful. deep neck infections causing a descending mediastinitis should be considered in patients even without an abnormal neck appearance.200818213468
detection and identification of bacterial dna in detect and identify bacteria in semen by sequencing polymerase chain reaction (pcr)-amplified ribosomal rna gene regions (rdnas).200818191853
european surveillance study on antimicrobial susceptibility of gram-positive anaerobic cocci.gram-positive anaerobic cocci (gpac) are a heterogeneous group of microorganisms frequently isolated from local and systemic infections. in this study, the antimicrobial susceptibilities of clinical strains isolated in 10 european countries were investigated. after identification of 299 gpac to species level, the minimum inhibitory concentrations of penicillin, imipenem, clindamycin, metronidazole, vancomycin and linezolid were determined by the agar dilution method according to the clinical and ...200818180149
generation and expression of a minimal hybrid ig-receptor formed between single domains from proteins l and g.the ig-binding properties of protein l from peptostreptococcus magnus and protein g from streptococcus have been successfully combined through the construction of a novel hybrid protein, consisting of a single ig-binding domain from each protein. the biophysical and biochemical properties of this construct have been characterized through equilibrium and pre-equilibrium fluorescence spectroscopy, circular dichroism, isothermal titration calorimetry, affinity chromatography, and conformational sta ...200818158250
[a case of bacteremia by atopobium rimae in a patient with liver cirrhosis].atopobium rimae, previously lactobacillus rimae, is a strictly anaerobic, non-spore forming grampositive rod which was frequently isolated from odontogenic infection. we report a case of a. rimae bacteremia. a 47-yr-old man with liver cirrhosis was admitted to the hospital via emergency room due to fever and chill. his abdominal and pelvic computed tomography revealed a small abscess near the left adrenal gland. three sets of blood cultures were taken and non-spore forming, grampositive rods wer ...200718094600
rapid identification of non-sporing anaerobes using nuclear magnetic resonance spectroscopy and an identification strategy.the non-sporing anaerobes cause a wide spectrum of infections. they are difficult to culture and their identification is tedious and time-consuming. rapid identification of anaerobes is highly desirable. towards this end, the potential of nuclear magnetic resonance (nmr) spectroscopy for providing a fingerprint within the proton spectrum of six genera belonging to anaerobes reflecting their characteristic metabolites has been investigated.200718087080
characterization of the protein unfolding processes induced by urea and temperature.correct folding is critical for the biological activities of proteins. as a contribution to a better understanding of the protein (un)folding problem, we studied the effect of temperature and of urea on peptostreptococcal protein l destructuration. we performed standard molecular dynamics simulations at 300 k, 350 k, 400 k, and 480 k, both in 10 m urea and in water. protein l followed at least two alternative unfolding pathways. urea caused the loss of secondary structure acting preferentially o ...200818065481
[co-occurence of indol-producing bacterial strains in the vagina of women infected with chlamydia trachomatis].the aim of this study was to determine if cervicitis, caused by chlamydia trachomatis (c. trachomatis), has an influence on the frequency of occurrence of selected aerobic and anaerobic bacterial strains, connected with etiology of aerobic vaginitis (av) and bacterial vaginosis (bv). indole-producing bacteria have received particular attention due to their possibly inductive role in chronic cervicitis caused by c. trachomatis.200718050609
cephalosporins in overcoming beta-lactamase-producing bacteria and preservation of the interfering bacteria in the treatment of otitis, sinusitis and tonsillitis.the treatment of upper respiratory tract infections (urtis) is complicated by the resurgence of beta-lactamase-producing bacteria (blpb) and the absence of interfering bacteria. blpb can have a direct pathogenic impact in causing the infection as well as an indirect impact through their ability to produce the enzyme beta-lactamase. blpb may not only survive penicillin therapy but can also protect other penicillin-susceptible bacteria from penicillin. in this review, the clinical in vitro and in ...200718039079
the bacteriology and antimicrobial susceptibility of infected and non-infected dog bite wounds: fifty bite wounds are a common reason for dogs requiring veterinary care, but there is surprisingly little data on the bacteriology of bite wounds. a prospective study was performed on dogs with various grades of bite wound to identify the bacteria present in these wounds. swabs were collected from all wounds for bacterial culture and cytology. all swabs were cultured aerobically and anaerobically and all aerobic cultures were evaluated for antibiotic susceptibility using the kirby bauer disk diff ...200818029118
[the development and in vitro experiment study of a bio-type root canal filling sealer using calcium phosphate cement].the purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (cpc), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal.200718004487
severe versus local odontogenic bacterial infections: comparison of microbial was the aim of this study to evaluate the clinical and microbiological differences between severe and local odontogenic abscesses.200817998782
extensive epidural abscess with surgical treatment and long term follow up.spinal epidural abscess is an uncommon infection. there are few reports on extensive epidural abscesses.200717998130
actinomycetemcomitin: a new bacteriocin produced by aggregatibacter (actinobacillus) actinomycetemcomitans.aggregatibacter (actinobacillus) actinomycetemcomitans p(7-20) strain isolated from a periodontally diseased patient has produced a bacteriocin (named as actinomycetemcomitin) that is active against peptostreptococcus anaerobius atcc 27337. actinomycetemcomitin was produced during exponential and stationary growth phases, and its amount decreased until it disappeared during the decline growth phase. it was purified by ammonium sulphate precipitation (30-60% saturation), and further by fplc (mono ...200817990016
anion modulation of the 1h/2h exchange rates in backbone amide protons monitored by nmr spectroscopy.the ability of three anionic cosolutes (sulfate, thiocyanate, and chloride) in modulating the (1)h/(2)h exchange rates for backbone amide protons has been investigated using nuclear magnetic resonance (nmr) for two different proteins: the igg-binding domain of protein l (protl) and the glucose-galactose-binding protein (ggbp). our results show that moderate anion concentrations (0.2 m-1 m) regulate the exchange rate following the hofmeister series: addition of thiocyanate increases the exchange ...200717965190
[late complications of a pseudo aneurysm of the left ventricle: thrombus infection and purulent pericarditis].pseudoaneurysm is a rare complication of left ventricle myocardial infarction. rupture with tamponade and sudden death is the usual outcome. surgical intervention remains the treatment of choice. long term survival cases without surgery are rare. infection of the thrombus is also a possible event. we report the case of a patient with postinfarction left ventricular pseudoaneurysm complicated by infection of the thrombus and purulent pericarditis involving a peptostreptococcus. infection must be ...200717963717
peptostreptococcus micros cell wall elicits a pro-inflammatory response in human macrophages.peptostreptococcus micros is a gram-positive anaerobic bacterium associated with periodontitis, a chronic inflammatory disease affecting tooth-supporting tissues. in the present study, we investigated the response of human macrophages to stimulation with a cell wall preparation from p. micros. in addition, the effect of the preparation on the phosphorylation of macrophage kinases was studied. the preparation, which was non-toxic for macrophages, significantly increased the secretion of the pro-i ...200717956940
bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography.the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography.200717949344
evaluation of the microbiota of primary endodontic infections using checkerboard dna-dna hybridization.the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp.200717949342
degradation of serine-containing oligopeptides by peptostreptococcus micros atcc 33270.microorganisms of peptostreptococcus micros are asaccharolytic, anaerobic gram-positive cocci that are frequently isolated from human oral sites such as periodontal pockets. preliminary study showed that several amino acids, including serine, enhanced slightly the growth of p. micros. therefore, we investigated the degradation of serine and serine-containing oligopeptides.200717949340
an improved picture of methyl dynamics in proteins from slowly relaxing local structure analysis of 2h spin relaxation.protein dynamics is intimately related to biological function. core dynamics is usually studied with 2h spin relaxation of the 13cdh2 group, analyzed traditionally with the model-free (mf) approach. we showed recently that mf is oversimplified in several respects. this includes the assumption that the local motion of the dynamic probe and the global motion of the protein are decoupled, the local geometry is simple, and the local ordering is axially symmetric. because of these simplifications mf ...200717941658
in vitro and in vivo antibacterial activities of dc-159a, a new fluoroquinolone.dc-159a is a new 8-methoxy fluoroquinolone that possesses a broad spectrum of antibacterial activity, with extended activity against gram-positive pathogens, especially streptococci and staphylococci from patients with community-acquired infections. dc-159a showed activity against streptococcus spp. (mic(90), 0.12 microg/ml) and inhibited the growth of 90% of levofloxacin-intermediate and -resistant strains at 1 microg/ml. the mic 90s of dc-159a against staphylococcus spp. were 0.5 microg/ml or ...200817938194
purification and partial characterization of a bacteriocin produced by eikenella corrodens.the purpose of this study was to purify and characterize a bacteriocin produced by eikenella corrodens a32e2.200817922820
breast abscess bacteriologic features in the era of community-acquired methicillin-resistant staphylococcus aureus epidemics.increasing rates of community-acquired methicillin-resistant staphylococcus aureus (mrsa) infections have also affected the microbial profile of breast abscesses.200717891866
an infected porous polyethylene orbital implant.a 75-year-old man underwent enucleation with placement of a porous polyethylene orbital implant (medpor, porex technologies, fairburn, ga, u.s.a.). over the next 5 years, he was seen on numerous occasions with socket discharge that was unresponsive to a variety of eyedrops. exposure and re-exposure of the implant occurred, and the implant was removed. histopathologic assessment was consistent with an infectious process within the implant. postoperatively, the patient's symptoms and signs resolve ...200717881998
probing the determinants of coenzyme specificity in peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis.glutamate dehydrogenase (ec from peptostreptococcus asaccharolyticus has a strong preference for nadh over nadph as a coenzyme, over 1000-fold in terms of kcat/km values. sequence alignments across the wider family of nad(p)-dependent dehydrogenases might suggest that this preference is mainly due to a negatively charged glutamate at position 243 (e243) in the adenine ribose-binding pocket. we have examined the possibility of altering coenzyme specificity of the peptostreptococcus enz ...200717850332
comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of fusobacterium nucleatum and peptostreptococcus (micromonas) micros.this study compared growth and susceptibility to different concentrations of sodium hypochlorite (naocl) of mono- and dual-species biofilms of fusobacterium nucleatum or peptostreptococcus micros in vitro at 24 hours or 96 hours. a mann-whitney u test revealed that although at 24 hours dual-species biofilms had similar viable counts to those of monospecies biofilms (p > 0.001), they were more resistant to naocl (p < 0.001). at 96 hours, both microorganisms had higher viable counts and were more ...200717804319
periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: a case-control study.recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. this case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (cad) based on clinical and periodontal microbiologic parameters.200717760542
development of 16s rrna-based probes for the identification of gram-positive anaerobic cocci isolated from human clinical specimens.fluorescent probes targeted at 16s rrna were designed for peptostreptococcus anaerobius and peptostreptococcus stomatis (pana134), parvimonas micra (pamic1435), finegoldia magna (fmag1250), peptoniphilus asaccharolyticus (pnasa1254), peptoniphilus ivorii (pnivo731), peptoniphilus harei (pnhar1466), anaerococcus vaginalis (avag1280) and anaerococcus lactolyticus (alac1438), based on the 16s rrna sequences of reference strains and 88 randomly chosen clinical isolates. these strains were also used ...200717714522
anaerobes in pleuropulmonary infections.a total of 76 anaerobes and 122 aerobes were isolated from 100 patients with pleuropulmonary infections, e.g. empyema (64), pleural effusion (19) and lung abscess (13). in 14% of the patients, only anaerobes were recovered, while a mixture of aerobes and anaerobes was encountered in 58%. from all cases of lung abscess, anaerobic bacteria were isolated, alone (04) or along with aerobic bacteria (13). from empyema and pleural effusion cases, 65.6% and 68.4% anaerobes were recovered respectively. a ...200217657055
actinomycotic bacteraemia after dental assess role of oral flora in causing bacteraemia following dental manipulations.200217657035
role of anaerobes in acute pelvic inflammatory disease.pouch of douglas aspirates were collected from 50 women with history and examination suggestive of acute pelvic inflammatory disease (pid) and 20 healthy women admitted for tubal ligation served as control. a total of 57 microorganisms were isolated from 37 patients out of 50 in study group. of 37 positive cultures 21(56.7%) were monomicrobial and 16(43.2%) were polymicrobial. most common symptom in study group was lower abdominal pain (90%), vaginal discharge (70%) and irregular bleeding (40%) ...200317643017
anaerobes in bacterial vaginosis.four hundred high vaginal swabs were taken from patients attending gynaecology and obstetrics department of govt. medical college, amritsar. the patients were divided into four groups i.e. women in pregnancy (group i), in labour/post partum (group ii), with abnormal vaginal discharge or bacterial vaginosis (group iii) and asymptomatic women as control (group iv). anaerobic culture of vaginal swabs revealed that out of 400 cases, 212(53%) were culture positive. maximum isolation of anaerobes was ...200317642997
microbial flora in orodental infections.the present study was carried out to compare the normal aerobic and anaerobic bacterial oral flora with flora from deep seated dental caries, gingivitis and adult periodontitis. all the samples belonging to both the control and study groups yielded microbes. aerobe / anaerobe ratio was high in normal flora (1.48) as compared to dental caries (0.9), gingivitis (0.72) and periodontitis (0.56). ninety seven percent of orodental infections were polymicrobial and three or more microbes were found in ...200317642993
bacteriology of diabetic foot lesions.clinical grading and bacteriological study of 107 patients with diabetic foot lesions revealed polymicrobial aetiology in 69 (64.4%) and single aetiology in 21 (19.6%). among 107 patients 62 had ulcer. of these 31 had mixed aerobes. twenty six patients with cellulitis and 12 with gangrene had more than 5 types of aerobes and anaerobes such as e. coli, klebsiella spp., pseudomonas spp., proteus spp., enterobactor spp., enterococci spp., clostridium perfringens, bacteroides spp., prevotella spp. a ...200417642727
the effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by m1 and m2 assess the influence of co-culture with mineral trioxide aggregate (mta) on phagocytosis and the production of reactive oxygen intermediates (roi) and nitrogen (no) species and the arginase activity by m1 and m2 peritoneal macrophages.200717627697
necrotizing fasciitis: a complication of percutaneous coronary revascularization.percutaneous coronary revascularization is rarely complicated by infection. necrotizing fasciitis (nf) is a severe, deep-seated, potentially life-threatening infection of fascia and subcutaneous tissues. we report herein 2 cases of nf in patients undergoing percutaneous transluminal coronary revascularization for treatment of acute coronary syndrome. these are the first 2 reported cases of nf associated with percutaneous coronary revascularization.200717626992
middle meatus bacteriology of acute rhinosinusitis in patients after irradiation of nasopharynx.patients who undergo radiotherapy for nasopharyngeal carcinoma (npc) tend to suffer from rhinosinusitis because irradiation causes damage to sinonasal tissue; however, their bacteriology is lacking in the literature. the aim of this study was to determine the bacteriology and antibiotic resistance in acute rhinosinusitis (ars) of these patients.200717621810
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004].tendency of isolated bacteria from infections in abdominal surgery during the period from april 2005 to march 2006 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 384 strains including 18 strains of candida spp. were isolated from 161 (70.3%) of 229 patients with surgical infections. one hundred and ninty-five strains were isolated from primary infections, and 171 strains were isolated from postoperative infections. from primary infecti ...200717612256
[the bacterial vaginosis--treatment problems].the changes in vaginal ecosystem (decreasing number of h2o2 producing lactobacillus spp. and increasing number of mobiluncus spp., gardnerella vaginalis, atopobium vaginae, prevotella spp., mycoplasma hominis, peptostreptococcus spp.) appears to be a major cause of bacterial vaginosis (bv). bacterial vaginosis is associated with infectious complications in pregnant and non-pregnant women and increasing risk of sti. the preventive role of natural vaginal microflora and problems of bv treatment ar ...200717607971
antimicrobial susceptibility of 800 anaerobic isolates from patients with dentoalveolar infection to 13 oral antibiotics.the aim of this study was to determine the current antimicrobial susceptibility of the principle anaerobic pathogens involved in dentoalveolar infection, to 13 oral antibiotics, and to assess the value of each antibiotic in the management of the infection.200717600542
production of antagonistic substance by eikenella corrodens isolated from the oral cavity of human beings with and without periodontal disease.antagonistic abilities may confer ecological advantages for micro-organisms in competitive ecosystems. however, reports regarding this phenomenon in eikenella corrodens are not available.200717584471
crystal structure of a bacterial albumin-binding domain at 1.4 a resolution.the albumin-binding domain, or ga module, of the peptostreptococcal albumin-binding protein expressed in pathogenic strains of finegoldia magna is believed to be responsible for the virulence and increased growth rate of these strains. here we present the 1.4a crystal structure of this domain, and compare it with the crystal structure of the ga-albumin complex. an analysis of protein-protein interactions in the two crystals, and the presence of multimeric ga species in solution, indicate the ga ...200717575979
microbiology of acute sinusitis of odontogenic origin presenting with periorbital cellulitis in children.this study was undertaken to evaluate the aerobic and anaerobic microbiology of acute maxillary sinusitis of odontogenic origin presenting with periodontal infection in children.200717561769
complicated parapneumonic effusion and empyema thoracis: microbiology and predictors of adverse describe the microbiological characteristics of a cohort of patients with complicated parapneumonic effusion and empyema thoracis, and to identify the potential risk factors for adverse outcomes, with particular reference to the choice of empirical antibiotics, intrapleural fibrinolytics, adherence to management guidelines, and input from pulmonologists.200717548905
prospective study of bacteremia and complications with eus fna of rectal and perirectal lesions.recent studies showed that endoscopic ultrasound (eus)-guided fine-needle aspiration (fna) is a low-risk procedure for causing bacteremia and infectious complications when sampling solid lesions of the upper gastrointestinal (gi) tract. as a result, antibiotics are not recommended for prophylaxis against endocarditis. our aim was to prospectively evaluate the risk of bacteremia and other infectious complications in patients undergoing eus fna of lower gi tract lesions.200717544995
[microbiocenosis of stomach in children with chronic gastritis].qualitative and quantitative composition of gastric microflora has been studied in 30 children with chronic gastritis. bacteriological study of gastric biopsies and parietal mucus in 10 children with chronic gastritis not associated with helicobacter pylori more frequently revealed conditionally pathogenic bacteria--escherichia coli, staphylococcus aureus, streptococcus viridans, s. pyogenes (group a), peptostreptococcus anaerobius, p. prevotii and their associations. in 20 studied patients with ...200717523484
in vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens.propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. in the present study, the antimicrobial activity of five propolis samples, collected from four different regions in turkey and from brazil, against nine anaerobic strains was evaluated. ethanol extracts of propolis (eep) were prepared from propolis samples and we determined minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of eep ...200717475517
microorganisms in root carious lesions in adults.root caries is emerging as a significant problem in the middle aged and elderly subjects because of the improving general health conditions, and medical and technological advances. the purpose of this investigation was to assess the prevalence of aerobic and anaerobic bacteria as well as yeasts of candida genus in root carious lesions in middle-aged and older adults.200617460839
bacterial composition in the supragingival plaques of children with and without dental caries.the purpose of the present investigation was to determine if the supragingival bacterial composition plaques in children with caries would differ from those found in caries-free controls.200617458088
aerobic and anaerobic bacteria in subgingival and supragingival plaques of adult patients with periodontal disease.clinical, epidemiological and microbiological examinations of adult patients with periodontal disease.200617458065
microbiological and immunological study of staphylococcus vaccine effects in periodontitis.the aim of this study was to evaluate the immunomodulatory effect of the staphylococcal vaccine inoculated subcutaneously in 15 patients with chronic periodontitis. bacteriological investigation of samples collected from the periodontal pocket for aerobic and anaerobic microorganisms was performed by classic bacteriological procedures before and after vaccination. the following immune system parameters were evaluated: c reactive protein (crp), serum level of c3 complement fraction, igg, iga, and ...200517405309
exploitation of host signaling pathways by b cell superantigens--potential strategies for developing targeted therapies in systemic autoimmunity.some infectious agents produce molecules capable of interacting specifically with the immunoglobulin heavy- or light-chain variable regions, independently of the conventional-binding site. they are referred to as b cell superantigens (sags) and include protein a of staphylococcus aureus (s. aureus), gp120 of hiv-1, and protein l of peptostreptococcus magnus (p. magnus). in contrast to conventional antigens, b cell superantigens interact with conserved framework regions of immunoglobulins and can ...200717404047
antimicrobial susceptibilities of peptostreptococcus anaerobius and the newly described peptostreptococcus stomatis isolated from various human sources.peptostreptococcus anaerobius sensu lato, currently including two closely related species, p. anaerobius and p. stomatis, is known to be more resistant than other gram-positive anaerobic cocci. we reidentified potential peptostreptococcus isolates and tested their susceptibilities to eight antimicrobials. notably, p. anaerobius had constantly higher values for the mic at which 50% of the isolates are inhibited (mic(50)) and the mic(90) than p. stomatis.200717403999
b cell superantigens subvert innate functions of b cells.some infectious agents produce molecules capable of interacting specifically with the immunoglobulin variable regions, independently of the conventional binding site. they are referred to as b cell superantigens, and include protein a of staphylococcus aureus, protein l of peptostreptococcus magnus, and gp120 of hiv-1. to test their effects in vivo, we used transgenic mice whose immunoglobulin loci were inactivated and their humoral immune system had been restored by introduction of human immuno ...200717369702
sirolimus-associated chronic pyogenic periungual infection. 200717344893
late abscess formation following indirect hernia repair utilizing silk suture.inguinal hernia repair is the most common operation performed by pediatric surgeons. the critical portion of the operation is high ligation of the hernia sac, which is classically performed with silk suture. this foreign body has the potential to serve as a nidus for latent infection/rejection that may result in an abscess long after the operation. this complication has rarely been described in the literature. we have cared for six children who have presented with latent inguinal or pelvic absce ...200717333211
clindamycin versus unasyn in the treatment of facial cellulitis of odontogenic origin in children.the study was undertaken to characterize the microbiology of dental abscesses in children and to compare clindamycin and ampicillin/sulbactam in the treatment of facial cellulitis of odontogenic origin. sixty children with acute facial cellulitis of dental origin underwent surgery (extraction or root canal procedure) within 24 hours of presentation. pus samples were cultured aerobically and anaerobically. patients were randomized (1:1) to receive intravenous ampicillin/sulbactam or clindamycin f ...200717325089
effect of smoking cessation on the microbial determine the effect of smoking cessation on the frequency of recovery of potential pathogens and aerobic and anaerobic interfering bacteria in the nasopharynges of smokers.200717309981
evaluation of subgingival bacteria in the dog and susceptibility to commonly used antibiotics.the aim of the present investigation was to evaluate the subgingival aerobic and anaerobic flora of 13 dogs with periodontal disease and the susceptibility of these bacteria to antibiotics currently approved in italy for treatment of canine infections. of the anaerobic bacteria, bacteroides fragilis was most frequently isolated, followed by peptostreptococcus + porphyromonas gingivalis and prevotella intermedia. of the aerobic bacteria, alpha-hemolytic streptococcus was most frequently isolated, ...200617286127
antianaerobe activity of ceftobiprole, a new broad-spectrum cephalosporin.agar dilution testing of 463 anaerobes showed most gram-positive beta-lactamase-negative strains (other than some clostridium difficile and peptostreptococcus anaerobius), as well as both beta-lactamase-positive and beta-lactamase-negative strains of fusobacterium nucleatum, to have ceftobiprole mic values of < or =0.016 to 4 microg/ml. ceftobiprole was less active against beta-lactamase-positive gram-negative bacilli, especially the members of the bacteroides fragilis group. like ceftobiprole, ...200717240107
hemin nutritional stress inhibits bacterial invasion of radicular dentine by two endodontic determine if anaerobic bacteria routinely found in infected dentine and root canals require the presence of heme in the environment in order for them to invade dentinal tubules.200717229113
microbiology of intracranial abscesses associated with sinusitis of odontogenic origin.the unique microbiology of sinusitis of dental origin that is associated with intracranial abscesses (ias) and the correlation between the organisms at the two sites has not been reported before. this report describes the author's experience during a 30-year period in studying the microbiology of 8 ias and their corresponding sinusitis of dental origin.200617214267
[liver abscess and infective endocarditis cases caused by ruminococcus productus].the genus ruminococcus which are anaerobe gram positive cocci, previously classified as peptostreptococcus, may colonize the upper respiratory tract, gastrointestinal tract, vagina and skin of humans and animals. in this report a case of liver abscess and a case of infective endocarditis caused by ruminoccocus productus, which is very rarely encountered in the clinical practice were presented. the first case was a 32 years old male who was admitted to the hospital in 2002, with the complaints of ...200617205698
comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction.saliva has been studied for the presence of subgingival pathogens in periodontitis patients. with the anaerobic culture technique, the discrepancy between salivary recovery and subgingival presence has been significant, which makes this approach not suitable for practical use in the microbial diagnosis of periodontitis patients. the real-time polymerase chain reaction (pcr) technique represents a very sensitive technique to detect and quantify bacterial pathogens. the aim of the study was to com ...200717199543
activities of four frog skin-derived antimicrobial peptides (temporin-1dra, temporin-1va and the melittin-related peptides ar-23 and rv-23) against anaerobic bacteria.the activities of two antimicrobial peptides belonging to the temporin family (temporin-1dra from rana draytonii and temporin-1va from rana virgatipes) and two peptides with structural similarity to the bee venom peptide melittin (ar-23 from rana tagoi and rv-23 from r. draytonii) were evaluated against a range of reference strains and clinical isolates of anaerobic bacteria. these peptides were selected because they show broad-spectrum growth inhibitory activity against reference strains of sev ...200717196372
human serum activity of telithromycin, azithromycin and amoxicillin/clavulanate against common aerobic and anaerobic respiratory pathogens.telithromycin is a new ketolide antimicrobial with a good in vitro activity against both aerobic and anaerobic respiratory pathogens. in this study, we evaluated the antibacterial activity over time of telithromycin (800mg), azithromycin (500mg), and amoxicillin/clavulanate (875/125mg) in serum following single oral doses of these agents to 10 healthy subjects. inhibitory and bactericidal titers were determined at 2, 6, 12, and 24h after each dose and the median titer was used to determine antib ...200717189093
phylogenetic analysis of bacterial and archaeal species in symptomatic and asymptomatic endodontic infections.phylogenetic analysis of bacterial and archaeal 16s rrna was used to examine polymicrobial communities within infected root canals of 20 symptomatic and 14 asymptomatic patients. nucleotide sequences from approximately 750 clones amplified from each patient group with universal bacterial primers were matched to the ribosomal database project ii database. phylotypes from 37 genera representing actinobacteria, bacteroidetes, firmicutes, fusobacteria and proteobacteria were identified. results were ...200717172525
[linezolid: antibacterial activity, clinical efficacy and resistance].linezolid is a synthetic antibiotic, the first available agent in a new class of antibiotic called the oxazolidinones, whose particular mechanism of action consists in inhibiting the initiation of protein synthesis. its spectrum of in vitro and in vivo activity includes staphylococci, streptococci, enterococci, corynebacteria and some anaerobic bacteria (peptostreptococcus, clostridium, and fusobacterium). the first therapeutic results were very encouraging, leading to the marketing of the produ ...200617162258
identification of bacteria associated with spreading odontogenic infections by 16s rrna gene determine the bacterial species associated with spreading odontogenic infections (sois).200717141534
changes in bacterial profile during amebiasis: demonstration of anaerobic bacteria in ala pus samples.little is known about the changes in gut resident flora during amebic colitis and amebic liver abscess (ala) caused by entamoeba histolytica infection. fecal samples from ala patients, from healthy e. histolytica negative and positive (asymptomatic) individuals, and from pre- and post-metronidazole-treated healthy volunteers and pus samples from ala patients were tested for the presence of various bacterial genera using 16s rrna-based primers. statistically significant reduction in lactobacillus ...200617123981
on the use of denaturing gradient gel electrophoresis approach for bacterial identification in endodontic infections.bacteria in infected root canals of teeth evincing chronic apical periodontitis lesions were identified by a polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) approach. dna was extracted from root canal samples, and part of the 16s rrna gene of all bacteria was amplified by pcr and separated by dgge, generating banding patterns representative of the community structure. twenty visible bands were cut out of the gel, re-amplified, and sequenced to provide identification. ...200717115203
suppurative thyroiditis. 200617098092
red autofluorescence of dental plaque autofluorescence of plaque and its relation to fluorescence of a single species in the biofilm was studied. fluorescence images of non-disclosed and disclosed plaque of 28 first-year students were captured. the plaque samples were assessed by culture methods and studied for red autofluorescence. species capable of producing red autofluorescence were cultivated from subjects with and without red plaque autofluorescence. red autofluorescence was observed from actinomyces odontolyticus, prevote ...200617063027
[characteristics of war wound infection].war wounds are the most complex type of non-targeted injuries due to uncontrolled tissue damage of varied and multifold localizations, exposing sterile body areas to contamination with a huge amount of bacteria. wound contamination is caused by both the host microflora and exogenous agents from the environment (bullets, cloth fragments, dust, dirt, water) due to destruction of the host protective barriers. war wounds are the consequence of destructive effects of various types of projectiles, whi ...200617048790
bacteriology of chronic sinusitis and acute exacerbation of chronic establish the microbiological characteristics of acute exacerbation of chronic sinusitis (aecs).200617043258
root canal microbiota of dogs' teeth with periapical lesions induced by two different methods.the microbial composition was investigated in root canals of dogs' teeth with periapical lesions induced by 2 different methods: open versus sealed canals.200616997126
in vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections.against 443 aerobic and anaerobic bacteria isolated from diabetic foot infections, ceftobiprole mics (microg/ml) at which 90% of the isolates tested were inhibited were as follows: methicillin-resistant staphylococcus aureus, 1; methicillin-susceptible s. aureus and staphylococcus lugdunensis, 0.5; anaerococcus prevotii, 0.125; finegoldia magna, 0.5; peptoniphilus asaccharolyticus, 1; peptostreptococcus anaerobius, 4; escherichia coli and enterobacter species, 0.125; klebsiella species, 2; and p ...200616982780
photodynamic therapy for endodontic disinfection.the aims of this study were to investigate the effects of photodynamic therapy (pdt) on endodontic pathogens in planktonic phase as well as on enterococcus faecalis biofilms in experimentally infected root canals of extracted teeth. strains of microorganisms were sensitized with methylene blue (25 microg/ml) for 5 minutes followed by exposure to red light of 665 nm with an energy fluence of 30 j/cm2. methylene blue fully eliminated all bacterial species with the exception of e. faecalis (53% kil ...200616982278
coaggregation interactions between oral and endodontic enterococcus faecalis and bacterial species isolated from persistent apical periodontitis.interactions between enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. the aim of this study was to investigate the coaggregation interactions between e. faecalis clinical isolates and species previously shown to survive and induce apical periodontitis in monkeys: peptostreptococcus anaerobius, prevotella oralis, fusobacterium nucleatum, and streptococcus anginosus. intergeneric coaggregation assay ...200616982270
[a case of multiple lung abscesses successfully treated with computed tomography guided percutaneous thoracic drainage].a 48-year-old woman who had abused alcohol admitted to our hospital because of fever and chest pain. laboratory data presented inflammation and liver dysfunction. chest x-ray films revealed multiple mass lesions with air fluid levels. we diagnosed multiple lung abscesses and started treatment with antibiotics. however, her fever did not improve and mass lesions increased. on day 6 of hospitalization, three drainage tubes were inserted percutaneously into the mass lesions under computed tomograph ...200616972615
microbiology of peritonsillar abscesses.the objective of the present study was to analyze the microbiology of peritonsillar abscesses.200616951860
recombinant production of a vl single domain antibody in escherichia coli and analysis of its interaction with peptostreptococcal protein l.a kappa-light chain from a fab expression system was truncated by the insertion of a stop codon in the gene sequence to produce a variable light (vl) single domain antibody (dab). here, we describe the expression of dab in the periplasm of escherichia coli through fermentation in a defined media. immunoglobulin binding domains from peptostreptococcal protein l (ppl) have been shown to bind specifically to kappa-light chains. we have produced recombinant ppl, at high yield, and this was used to c ...200716949300
periodontal status in smokers and never-smokers: clinical findings and real-time polymerase chain reaction quantification of putative periodontal pathogens.smoking is a well-known risk factor for destructive periodontal disease, but its relationship with periodontal status and subgingival microbiota remains unclear. inherent limitations of microbiological methods previously used may partly explain these mixed results, and real-time polymerase chain reaction (pcr) has been presented as a valid alternative. the aim of the present study was to investigate the clinical condition and microbiological profile of patients with chronic periodontitis as rela ...200616945023
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