PMID(sorted ascending)
isolation and identification of intestinal bacteria from japanese tree frog (hlya japonica) with the special reference to anaerobic bacteria.the bacteria in the large intestines of eight japanese tree frogs (hlya japonica) were enumerated by using an anaerobic culture system. the microorganisms at approximately 3.1 x 10(9) bacteria per g (wet weight) of intestinal contents were present in the intestine of all the frogs tested. no difference of the total bacteria in the frog intestine was observed between two different incubation-temperatures (room temperature and 37 degrees c). eleven genera and 16 species were isolated from the frog ...19921391180
impact of the advances in age on the gastrointestinal microflora of beagle dogs.the gastrointestinal microflora of male beagle dogs in two different age groups; i) less than month 12 of age, and ii) more than year 11 of age, was compared. no detectable difference occurred on the microflora of stomach, duodenum, jejunum, and ileum in both dogs. large bowel (cecum, colon and rectum) microflora in both dogs yielded the different microbial populations. in all regions of large bowel, the levels of bacteroides, eubacteria, peptostreptococci, bifidobacteria, lactobacilli, and stap ...19921391181
bacteriology of periodontal disease.the microbial flora associated with the periodontal tissues in health and disease is extremely complex, and much research is being directed toward identifying those species that may be etiologic agents or that can be used as prognostic indicators. recent work has resulted in changes in the taxonomic position of several periodontal species and the recognition that several others, particularly species of eubacterium and peptostreptococcus, as well as a novel oral spirochete, may be important in di ...19921392003
bacterial invasion of non-exposed dental pulp.anaerobic procedures were adopted to demonstrate the early bacterial invasion of non-exposed dental pulps, and to isolate and identify the bacteria. of 19 freshly extracted teeth which originally exhibited deep dentinal lesions, clinical examination and electric pulp testing showed that nine of them had no pulpal exposure. thus the pulps of these teeth were covered by clinically sound dentine beneath the carious lesion. bacteria were found to have invaded the pulps of six of these nine teeth. th ...19921399049
microbiology of secondarily infected diaper dermatitis.specimens obtained from 67 infants with secondarily infected diaper dermatitis were cultured for aerobic and anaerobic bacteria. bacteria growth was obtained in 58. aerobic facultative bacteria or candida sp. only were present in 28 patients (48%), anaerobic bacteria only in 11 (19%), and mixed anaerobic with aerobic, facultative, or yeast flora was present in 19 (33%). ninety-one bacterial or fungal isolates were recovered (1.6 per specimen), 54 (0.9 per specimen) aerobic or facultative bacteri ...19921399196
anaerobic bacterial infections causing osteomyelitis/arthritis in a dog.a 3-year-old german shepherd dog was examined for lameness, signs of pain, swelling, a draining fistulous tract, and osteolysis after a dog bite on the left carpus. after failure of the lesion to respond to several antibiotics, peptostreptococcus sp and propionibacterium sp were isolated from swab specimens and then from surgically collected bone and soft tissue specimens. the bone fragments had mild purulent osteomyelitis associated with numerous gram-positive rods and cocci. the dog was succes ...19921399798
enzymatically active peptostreptococcus magnus: association with site of infection.fifty-four strains of peptostreptococcus magnus (11 were recovered from abdominal infections, 18 were from nonpuerperal breast abscesses, and 21 were from diabetic foot infections; the type strain and three other strains were from the american type culture collection, rockville, md.) and the type strain of peptostreptococcus micros were tested for their ability to produce various enzymes, including catalase, hippurate hydrolase, serine dehydratase, threonine dehydratase, collagenase, gelatinase, ...19921400997
necrotizing fasciitis of the neck and chest. report of a case.a case is presented of necrotizing fasciitis of the neck and chest characterized by rapid progressive necrosis of subcutaneous tissue, fascia and skin. the diagnosis and management is discussed.19921402054
[asporogenic anaerobic thoracic empyema--an experimental model for anaerobic thoracic empyema in rabbits].sterile pleural effusion was induced by intrapleural administration of turpentine, and empyema was induced by direct intrapleural inoculation of bacteria in rabbits. experimental models of anaerobic thoracic empyema were successfully made in this study, using escherichia coli, peptostreptococcus asaccharolyticus, bacteroides fragilis, using the following methods; (1) a single bacterium inoculation of, a) e. coli, b) p. asaccharolyticus, c) b. fragilis, (2) simultaneous inoculations with an aerob ...19921402083
[bacteriology of infectious disease in otorhinolaryngology (1). bacteriological study of paranasal cyst].the bacteriology of chronic sinusitis has been reported in several studies, but there have been few bacteriological studies on paranasal cyst. also, details of the techniques of transportation and processing of clinical specimens have only rarely been described. therefore, this study was undertaken with attention to the above, and to obtain information on appropriate antimicrobial therapy, we conducted a dilution antimicrobial susceptibility test of isolates. fifteen patients with paranasal cyst ...19921403316
experimentally induced infection by oral anaerobic microorganisms in a mouse model.a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ...19921408362
anaerobic microflora associated with the pars oesophagea of the healthy pigs aged one to 35 days significant populations of microorganisms (5.5 to 6.9 log10 viable count cm-2 of the tissue) were found, by strict anaerobic techniques, to adhere to the pars oesophagea. the genera of anaerobes which were isolated included actinomyces, bifidobacterium, clostridium, lactobacillus, peptostreptococcus, streptococcus and veillonella and facultative anaerobes included escherichia, klebsiella, staphylococcus, streptococcus and yeasts. the microbial population adher ...19921410807
[experience with the management of endometritis in the instituto nacional de perinatalogía].endometritis is the most frequent infectious complication of the puerperal period; with the objective to know the clinical characteristics, etiology and evolution of this disease we did a retrospective study of the endometritis cases among the patients of the instituto nacional de perinatología (inper) from january 1st, 1990 to may 31, 1991. during the revision period were diagnosed 120 cases, but we were able to examine only 90. in the 90% the resolution of the pregnancy was by means of caesare ...19921427285
abnormal intracerebral thallium localization in a bacterial brain abscess.a 56-yr-old man with a prior history of renal cell carcinoma had moderately intense abnormal localization of 201tl-chloride in a solitary brain lesion which was discovered to be a bacterial abscess. the organisms isolated by culture included actinomycosis odontolyticus, peptostreptococcus and hemophilus aphrophilus. because of the clinical presentation, mri characteristics and thallium scintigraphic findings, the lesion had been felt to represent either a primary or a metastatic neoplasm. this c ...19921432164
antibiotic susceptibility of anaerobic bacteria isolated in vitro susceptibilities of 198 anaerobic bacteria to seven antibiotics were evaluated by the agar dilution method. in addition to testing amoxycillin/clavulanic acid in a 2:1 ratio against the bacteria, the combination was also tested against 63 isolates using fixed concentrations of clavulanic acid and serial dilutions of amoxycillin. penicillin and cefoxitin were not effective against beta-lactamase-producing bacteroides isolates and only 50% of isolates were susceptible to the 2:1 amoxycill ...19921448710
aerobic and anaerobic microbiology of external otitis.microbiological and clinical data from 46 patients with external otitis were retrospectively evaluated. specimens were processed for isolation of aerobic and anaerobic bacteria. a total of 42 aerobic bacteria, 22 anaerobic bacteria, and 3 candida albicans organisms were recovered. aerobic bacteria only were isolated from 31 patients (67%), anaerobic bacteria only were isolated from 8 (17%), and mixed aerobic and anaerobic bacteria were isolated from 4 (9%). c. albicans alone was recovered from t ...19921457667
protein lg: a hybrid molecule with unique immunoglobulin binding properties.immunoglobulin (ig)-binding bacterial proteins have attracted theoretical interest for their role in molecular host-parasite interactions, and they are widely used as tools in immunology, biochemistry, medicine, and biotechnology. protein l of the anaerobic bacterial species peptostreptococcus magnus binds ig light chains, whereas streptococcal protein g has affinity for the constant (fc) region of igg. in this report, ig binding parts of protein l and protein g were combined to form a hybrid mo ...19921460053
prospective study of blood culture during transesophageal ascertain the incidence and significance of bacteremia associated with transesophageal echocardiography (tee), 132 consecutive patients (aged 17 to 73 years) free of apparent infection who were undergoing 135 transesophageal echocardiographic procedures from october 1990 to august 1991, were prospectively studied. for each procedure, two sets of blood cultures were obtained for culture 30 to 60 minutes before tee, immediately after, and 180 to 240 minutes after the procedure. for each blood c ...19921462911
experimental studies of survival of anaerobic bacteria at 4 degrees c and 22 degrees c in two different transport systems.the survival of anaerobic bacteria on swabs in two different transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart transport medium (mst), and a viscose swab in modified siff transport medium (btm) (bionor). the following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; fusobacterium necrophorum, bacteroides melaninogenicus, bacteroides inte ...19921472363
the relative lethality of intestinal bacteria for gnotobiotic rats with experimental intestinal eight experiments utilizing 28 animals each, germfree rats with and without ischemically or hemorrhagically strangulated closed loop intestinal segments were contaminated with either one of four common intestinal anaerobic bacteria, or a combination of two or three bacteria previously found to be innocuous in pure culture. the results showed that: a) in pure culture, bacteroides fragilis, bacteroides melaninogenicus, and peptostreptococcus anaerobius were innocuous; b) fusobacterium necrophor ...19921479304
the endodontic microflora revisited.the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ...19921479923
susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase.the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ...19921481764
comparative in vitro activity of ro 40-6890, ro 41-3399, and other antimicrobial agents against anaerobic bacteria.the in vitro activity of the ester ro 41-3399 and its free active acid ro 40-6890 was tested against 189 strains of anaerobic bacteria in comparison to other oral cephalosporins and to antimicrobial agents established in the treatment of anaerobic infections. prevotella, porphyromonas, peptostreptococcus, fusobacterium and clostridium spp. were susceptible to ro 40-6890, with few exceptions. due to its lack of activity against the major pathogens of the bacteriodes fragilis group, ro 40-6890 doe ...19921486894
physico-chemical and structural properties of the surfaces of peptostreptococcus micros and streptococcus mitis as compared to those of mutans streptococci, streptococcus sanguis and streptococcus salivarius.the surface properties of nine streptococcus mitis and four peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. in addition, elemental surface concentration ratios were obtained via x-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. the s. mitis and p. mi ...19921487734
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
prevention of malodor from intraoral gauze tamponade with the topical use of clindamycin.azulene ointment with a small dose of clindamycin was used topically in eight patients with maxillary cancer with the intent to inhibit the malodor that originates from a gauze tamponade applied to the postoperative maxillary bony defect. the malodor was either markedly decreased or eliminated in all cases. concurrent with the decrease or elimination of the malodor, anaerobic microorganisms such as bacteroides and peptostreptococcus, which are considered to be involved in the generation of malod ...19921508511
[pharmacokinetic, bacteriological, and clinical studies on panipenem/betamipron in children].pharmacokinetic, bacteriological and clinical studies were performed on panipenem/betamipron (papm/bp) in children. the results are summarized as follow: 1. twelve patients with various bacterial infectious diseases were treated with papm/bp. each dose was 20 mg/20 mg/kg, administered 3 times daily, in 30-minute intravenous drip infusion. treatments were continued for 5-22 days. clinical efficacies of papm/bp in 12 patients with bacterial infections (1 with suspected sepsis, 5 with pneumonia, 1 ...19921512926
comparative evaluation of the metabolic potentials of different strains of peptostreptococcus productus: utilization and transformation of aromatic compounds.three strains of peptostreptococcus productus were tested for growth at the expense of methoxylated aromatic compounds. strain m8a-18 (human fecal isolate) was unable to utilize methoxylated aromatic compounds. while the type strain atcc 27340 (human septicemia isolate) was capable of minimal growth with methoxylated aromatic compounds, atcc 35244 (sewage sludge isolate) displayed significant growth on methoxylated aromatic compounds. methoxylated phenols, benzoates, benzyl alcohol and phenylacr ...19921521774
[antimicrobial activities of ceftriaxone against clinically isolated strains].antibiotic activities (mics) of ceftriaxone (ctrx) against 1,210 strains of bacteria including 28 spp. isolated in 1987 and 1990 were compared with those of other cephems. 1. when compared to data on clinically isolated strains reported in the early 1980s, strains of the following species isolated in 1990 showed extremely elevated mic90s of ctrx: staphylococcus spp., streptococcus pneumoniae, escherichia coli, citrobacter spp., enterobacter spp., serratia spp., proteus vulgaris, morganella morga ...19921522669
peptostreptococcus micros in human periodontitis.peptostreptococcus micros is a recognized pathogen in medical infections, and its association with progressive periodontitis was examined in this study. p. micros was isolated from paper-point subgingival samples on anaerobic enriched blood agar plates and identified on the basis of cellular and colonial morphology and selected biochemical tests. in a cross-sectional study involving 907 people with advanced adult periodontitis, 127 with early-onset periodontitis, and 12 with localized juvenile p ...19921528618
enumeration of subgingival species on primary isolation plates using colony lifts.this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ...19921528619
serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin.numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ...19921528630
predominant obligate anaerobes in human periodontal pockets.this study was carried out to investigate the predominant anaerobic bacteria of periodontal pockets in patients with advanced periodontitis, who had no previous treatment other than supragingival scaling, no history of recent or chronic systemic illness, nor any intake of antibiotics within 6 weeks prior to bacteriological sampling. care was taken not to ignore tiny-colony-forming anaerobes, by means of a stereoscope and an anaerobic glove box system. out of 422 (100%) isolates, 380 (90%) were o ...19921531505
in vitro activities of three semisynthetic amide derivatives of teicoplanin, mdl 62208, mdl 62211, and mdl 62873.mdl 62208, mdl 62211, and mdl 62873 are three semisynthetic amide derivatives of teicoplanin (mdl 62208 is an amide of teicoplanin aglycone, mdl 62211 is an amide of the teicoplanin a2 complex, and mdl 62873 is the corresponding derivative of peak a2-2 of the complex). the three semisynthetic glycopeptides were evaluated for in vitro antibacterial activity in comparison with the parent drug (teicoplanin) and vancomycin. a variety of gram-positive bacteria of clinical origin, whose species were c ...19921534977
diagnosis and management of parotitis.of all the salivary glands, the parotid gland is most commonly affected by an inflammatory process. infections of the parotid gland range from acute to severe. assessment of the disease process should differentiate local primary parotid infection from systemic infection when this gland is also involved as part of a generalized inflammatory condition. viral parotitis can be caused by paramyxovirus (mumps), epstein-barr virus, coxsackievirus, and influenza a and parainfluenza viruses. acute suppur ...19921571113
the iron-sulfur-cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus. stereochemistry of the deamination of l-threonine.the stereochemistry of the deamination of l-threonine to 2-oxobutyrate, catalyzed by purified l-serine dehydratase of peptostreptococcus asaccharolyticus, was elucidated. for this purpose the enzyme reaction was carried out with unlabelled l-threonine in 2h2o and in 3hoh, as well as with l-[3-3h]threonine in unlabelled water. isotopically labelled 2-oxobutyrate thus formed was directly reduced in a coupled reaction with l- or d-lactate dehydrogenase and nadh. the (2r)- or (2s)-2-hydroxybutyrate ...19921572371
susceptibilities of anaerobic bacteria isolated from animals with ovine foot rot to 28 antimicrobial agents.the agar dilution method was used to determine the inhibitory activities of 28 antimicrobial agents against 35 strains of the genus peptostreptococcus, 4 strains of the species peptococcus niger, 20 strains of the species megasphaera elsdenii, 7 strains from the species acidaminococcus fermentans, 8 strains of the genus clostridium, 11 strains of the genus eubacterium, and 1 strain of the species propionibacterium acidipropionici, all of which were isolated from 125 clinical cases of ovine foot ...19921590689
the effect of amoxycillin on vaginal colonization resistance and normal vaginal flora in monkeys.previous studies have shown that vaginal colonization resistance in monkeys can be eliminated by amoxycillin and restored by flushing vaginal flora from a healthy monkey into the vagina of a monkey colonized with escherichia coli. the hypothesis that the effect of amoxycillin resulted from elimination of parts of the normal flora was tested in the present study. nine monkeys were flushed vaginally with amoxycillin daily for six days. the number of anaerobic bacteria decreased during amoxycillin ...19921592701
necrotizing fasciitis of the vulva.we attempted to characterize the natural history of necrotizing fasciitis of the vulva.19921595786
physicochemical and structural investigation of the surfaces of some anaerobic subgingival bacteria.the surfaces of nine clinical isolates of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and peptostreptococcus micros and that of laboratory strain p. gingivalis w83 were studied by using contact angle measurements, x-ray photoelectron spectroscopy, infrared spectroscopy, microelectrophoresis of whole cells, and transmission electron microscopy of whole and sectioned cells. p. intermedia strains were hydrophilic, as judged from their small water contact a ...19921599251
spontaneous gas-forming bacterial myonecrosis caused by group b streptococci and peptostreptococci.we present the case of a patient who spontaneously developed a severe gas-forming infection involving the voluntary muscles of both thighs. the organisms responsible were group b beta-haemolytic streptococci together with peptostreptococci. following surgical and medical treatment, the patient fully recovered. no predisposing factors, in particular no disease causing immunocompromise, could be identified.19921600357
modulation of human neutrophil adherence by oral bacteria.polymorphonuclear neutrophils (pmns) comprise over 90 per cent of leukocytes in the oral cavity. although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. adherence of pmns to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. in this study, oral bact ...19921605750
prosthetic valve endocarditis and paravalvular abscess caused by peptostreptococcus magnus. 19921617069
[gynecologic and obstetric infections caused by aerobic bacteria].the anaerobic bacteria (ab) are between the most numerous microorganisms (mo) that constitute the flora of the female genital tract, so they can participate in the etiology of obstetric and gynecologic infections (ogi). the objective of this study was to investigate the frequency of ab isolations and the clinical characteristics of the anaerobic infections (ai) in patients of the national institute of perinatology, from january 1st, 1988 to may 31, 1991. ab were isolated from 117 patients who de ...19921618410
structure of peptostreptococcal protein l and identification of a repeated immunoglobulin light chain-binding domain.the gene for protein l, an immunoglobulin (ig) light chain-binding protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus, was cloned and sequenced. the gene translates into a protein of 719 amino acid residues. following a signal sequence of 18 amino acids and a nh2-terminal region ("a") of 79 residues, the molecule contains five homologous "b" repeats of 72-76 amino acids each. further, toward the cooh terminus, two additional repeats ("c") were found. t ...19921618782
resistance of peptostreptococcus spp. to macrolides and lincosamides: inducible and constitutive phenotypes.the activities of erythromycin and clindamycin against 350 peptostreptococcus strains were studied during a 5-year period (1986 to 1991). in 5.1% of the peptostreptococcus strains, which presented dissociated resistance (clindamycin mic, less than or equal to 1 microgram/ml; erythromycin mic, greater than 8 micrograms/ml), evidence of inducible macrolide-lincosamide resistance was shown. a total of 17.7% of the strains presented a constitutive phenotype; the clindamycin and erythromycin mics for ...19921622181
[pyogenic liver abscess. its exclusive treatment with antibiotics]. 19921626100
[an increased incidence of bacterial endocarditis in chronic inflammatory bowel diseases].of 92 consecutive patients treated for proven native valve endocarditis three had ulcerative colitis and 2 crohn's disease. all 5 patients developed severe complications; three had to undergo emergency valve replacement. with a prevalence of 64.1/10(5) cases of inflammatory bowel disease the calculated incidence (5/92) revealed a significant over-representation of inflammatory bowel disease among patients with proven endocarditis (p less than 5.08 x 10(-9)). possible explanations may be the supp ...19921636271
spontaneous contrast echoes in pericardial effusion: sign of gas-producing infection. 19921636601
defective neutrophil function in an insulin-dependent diabetes mellitus patients. a case report.the objective of this study was to evaluate the polymorphonuclear leukocyte (pmn) function in a poorly controlled adult insulin-dependent diabetic patient (iddm) with severe recurrent periodontitis, while describing the microbiological and clinical findings. chemotaxis, superoxide production, and phagocytosis and killing of porphyromonas (bacteroides) gingivalis by the iddm pmn were evaluated 1 week before treatment relative to a healthy, matched control. these analyses revealed a significant (p ...19911651389
[strict anaerobic bacteria: comparative study of various beta-lactam antibiotics in combination with tazobactam or sulbactam].the minimal inhibitory concentrations of piperacillin (pip) or cefotaxime (ctx) alone or in combination with tazobactam (taz) were determined against 168 anaerobes. all the strains were inhibited by pip + taz, but certain strains resistant to ctx + taz were found among b. fragilis, eubacterium and peptostreptococcus. the second investigations included 30 strains of bacteroides fragilis. concentrations of 2, 4 and 8 mg/l of taz and sulbactam (sul) were combined with piperacillin or cefotaxime. th ...19911652729
subgingival microflora of advanced periodontitis in the dominican republic.a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ...19911658290
relation of baseline microbial parameters to future periodontal attachment loss.the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...19911661304
in-vitro activity of temafloxacin against anaerobic bacteria: a comparative study.the antimicrobial activity of temafloxacin for 328 anaerobic bacteria was determined and compared to that of cefotetan, cefoxitin, ciprofloxacin and metronidazole. the wadsworth agar dilution technique using brucella-lysed sheep blood agar was used throughout. at the recommended breakpoint concentration 4 mg/l, temafloxacin inhibited 60/62 (97%) of the isolates of bacteroides fragilis and 82/87 (94%) of the isolates of other species of the b. fragilis group. ninety-six percent of the 24 isolates ...19911664827
[anaerobes in infected canals: a preliminary study].anaerobes of 17 infected canals with periapical periodontitis were studied. most of root flora were not only anaerobes but also aerobes. anaerobes were predominant in chronic periapical periodontitis. major anaerobes isolated from canals were peptostreptococcus, b. melaninogenicus and b. oralis. the chi-square results indicated that the peptostreptococcus were significantly related to apical radiolucency and b. melaninogenicus were significantly related to percussion or foul smell. animal experi ...19911674465
percutaneous drainage of lung abscess in a malnourished child. 19911676504
[acute subdural empyema due to peptostreptococcus].a very rare case of acute subdural empyema due to peptostreptococcus was reported. a 11-year-old-girl was admitted to our hospital with high grade fever, unconsciousness and rt hemiparesis. ct scans showed the mass effect caused by the subdural empyema over the left frontotemporal region. subdural empyema was evacuated by the craniotomy. peptostreptococcus was found in the pus obtained during the operation. however, ct scans 10 days after the operation revealed another subdural empyema in the le ...19911681835
differential effects of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor in children with severe congenital neutropenia.severe congenital neutropenia (scn) is a disorder of myelopoiesis characterized by severe neutropenia secondary to a maturational arrest at the level of promyelocytes. we treated five patients with scn with recombinant human granulocyte-macrophage colony-stimulating factor (rhgm-csf) for 42 days and subsequently, between 1 and 3 months later, with rhg-csf for 142 days. the objective was to evaluate the safety and ability of these factors to elicit a neutrophil response. rhgm-csf was administered ...19901689595
protein l. a bacterial ig-binding protein that activates human basophils and mast cells.peptostreptococcus magnus strain 312 (10(6) to 10(8)/ml), which synthesizes a protein capable of binding to kappa l chains of human ig (protein l), stimulated the release of histamine from human basophils in vitro. p. magnus strain 644, which does not synthesize protein l, did not induce histamine secretion. soluble protein l (3 x 10(-2) to 3 micrograms/ml) induced histamine release from human basophils. the characteristics of the release reaction were similar to those of rabbit igg anti-fc frag ...19901698870
molecular fingerprinting of isolates of the genus peptostreptococcus using rrna genes from escherichia coli and p. anaerobius.restriction fragment length polymorphisms (rflps) of rrna genes were evaluated as a tool for intra- and interspecies differentiation of peptostreptococcus isolates. rflps from a collection of 20 clinical isolates and five atcc strains representing five peptostreptococcus spp. (p. anaerobius, p. asaccharolyticus, p. magnus, p. micros and p. prevotii) were obtained by hybridization of southern blots of hindiii- or ecori-digested genomic dna with three probes: probe a, a 0.98 kb hindiii fragment wi ...19911717639
diagnosis and management of anaerobic infections of the head and neck.anaerobic bacteria are important pathogens in head and neck infections such as chronic otitis media, chronic sinusitis, chronic mastoiditis, head and neck abscesses, cervical adenitis, parotitis, and postoperative infection. bacteroides sp (bacteroides melaninogenicus group, bacteroides oralis, and bacteroides fragilis group), peptostreptococcus sp, and fusobacterium sp predominate. the observed recent increase in the number of beta-lactamase-producing strains of bacteroides sp isolated in head ...19921728904
changes in the microbial flora of airline headset devices after their use.the bacterial flora of 20 headset devices were evaluated before and after they were worn for 1 hour. bacteria were recovered from all headsets, and their number increased from a mean (+/- standard deviation) of 60 +/- 5 organisms per device to 650 +/- 51 organisms per device (p less than .001). the predominant organisms recovered were staphylococcus spp, streptococcus spp, propionibacterium spp, and peptostreptococcus spp. this study demonstrates the presence of potential pathogens in headset de ...19921731163
protein l from peptostreptococcus magnus binds to the kappa light chain variable domain.protein l is an immunoglobulin light chain-binding protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the major variable region subgroups of human kappa and lambda light chains were tested for protein l binding; v kappa i, v kappa iii, and v kappa iv bound protein l, whereas no binding occurred with proteins of the v kappa ii subgroup or with any lambda light chain subgroups. studies of the protein l binding capacity of naturally occurring vl fragment ...19921733930
multicentre study on antibiotic susceptibilities of anaerobic bacteria to cefoperazone-sulbactam and other antimicrobial agents.the antibiotic susceptibilities of 241 anaerobic bacteria recovered from six geographic sites in north america were tested by agar dilution to cefoperazone-sulbactam and other drugs. of the 189 bacteroides fragilis group isolates, only one was resistant to cefoperazone-sulbactam (0.5%) or ampicillin-sulbactam (0.5%), and none was resistant to ticarcillin-clavulanate or chloramphenicol. no resistance to cefoperazone-sulbactam was observed among the other bacteroides spp., clostridium spp., or pep ...19921737725
the relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women.lactobacilli provide an important microbial defense against genital colonization by pathogens. the role of hydrogen peroxide (h2o2) in the control of genital microflora was explored in a cross-sectional study of 275 women in the second trimester of pregnancy. vaginal cultures were obtained for detection of h2o2-positive and h2o2-negative lactobacilli and other members of the genital microflora. compared with women with h2o2-negative lactobacilli, women colonized by h2o2-positive lactobacilli wer ...19921738516
severe anaerobic necrotizing pneumonia complicated by pyopneumothorax and anaerobic monoarthritis due to peptostreptococcus magnus.a rare case of a severe anaerobic necrotizing pneumonia complicated by pyopneumothorax and monoarthritis due to anaerobic peptostreptococcus magnus is described in a 20-year-old soldier. the patient's immunological status was normal. there were no predisposing underlying factors, and he was treated successfully without any residual damage. to our knowledge, a similar case has never been reported in the literature.19911745861
bacterial vaginosis.bacterial vaginosis (bv) is the most common of the vaginitides affecting women of reproductive age. it appears to be due to an alteration in the vaginal ecology by which lactobacillus spp., the predominant organisms in the healthy vagina, are replaced by a mixed flora including prevotella bivia, prevotella disiens, porphyromonas spp., mobiluncus spp., and peptostreptococcus spp. all of these organisms except mobiluncus spp. are also members of the endogenous vaginal flora. while evidence from tr ...19911747864
the microflora of periodontal sites showing active destructive progression.20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ...19911752997
[chronic conjunctivitis caused by oral anaerobic germs].a patient with chronic, unilateral conjunctivitis is presented. the predominant bacterial flora consisted mainly of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. restriction endonuclease fingerprinting of genomic dna demonstrated that p. intermedia most probably originated from the oral cavity. in the oral cavity this species was recovered from the dorsum of the tongue, the tonsillar area and the periodontal pockets. topical treatment with various antibi ...19911758517
[a classic case of ludwig's angina].the aa. have the chance to review the etiology, the clinic and the management of this risky process, through a characteristic ludwig's angina of their own practice. a middle age man carrying an odontogenic inflammation developed a spreading infection into the deep neck tissues. the problem was successfully resolved with antibiotherapy and surgical drainage.19911781512
[analysis of 98 cases of thoracic empyema].ninety-eight cases of empyema thoracis admitted to juntendo university hospital between 1979 and 1990 were reviewed. males accounted for 78 cases and females 20 cases. thirteen pediatric patients ranged in age from 17 days to 4 years, while the 85 adult cases ranged from 16 to 89 years (mean: 58.4 years). the mortality rate increased with age. fifty-three cases of community-acquired empyema thoracis consisted of 24 with no underlying disease (including 13 pediatric cases), and 29 with diabetes m ...19911783810
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta).the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ...19911803013
[effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections].the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ...19911805697
[empyema due to an anaerobic streptococcus in childhood].anaerobic infections are quite rare in pediatric age, being that, they affect only neonates and immunodepressed patients. we think to be somewhat interesting to describe the case of our patient, a 9 year old boy, unaffected by any predisposing factor, came under our observation because of a severe respiratory distress. he showed evident clinical and radiological signs of pleural effusion in the right lung, together with a gas coil in the upper field and a left mediastinal shifting. a thoracentes ...19911806927
bacteria in the apical 5 mm of infected root canals.ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...19911809801
formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria.during the course of our studies on the metabolism of iridoid glycosides by human intestinal bacteria, we found that geniposide (1) and gardenoside (4) were transformed to new nitrogen-containing compounds, genipinine (3) and gardenine (6), respectively, along with the known aglycones. although the amounts of new metabolites were somewhat lower than those of the aglycones, they were quantitatively analyzed by means of liquid chromatography/mass spectrometry (lc/ms). of 25 strains of human intest ...19911818345
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
native valve infective endocarditis in adults--analysis of 32 consecutive cases over a ten-year period from 1980 to 1989.thirty-two patients with native valve infective endocarditis who presented over a 10-year period at our hospital were analyzed retrospectively. the presenting symptom was a persistent fever in 22 patients. in 30 patients, the new york heart association functional class was less than ii before the development of endocarditis. blood cultures were positive on all occasions in 24 out of 29 culture-positive patients. all of the viridans streptococci, accounting for 79% of the isolated pathogens, were ...19911829492
microbial risk indicators for periodontal attachment loss. 19911831856
clinical and microbiological findings on newly inserted hydroxyapatite-coated and pure titanium human dental implants.the clinical and microbiologic features of 30 hydroxyapatite-coated root-form endosseous dental implants (tri-stage) were compared to 10 similar pure titanium implants without hydroxyapatite coatings. in 7 of 9 partially edentulous patients studied, pure titanium fixtures were placed adjacent to hydroxyapatite-coated implants. implants in the maxilla were submerged beneath mucosal tissues after implant placement for a minimum of 6 months, and in the mandible for at least 4 months. all patients w ...19911843465
microbial differences in 2 clinically distinct types of failures of osseointegrated implants.the microflora associated with osseointegrated implants suspected of failing from infection or trauma were evaluated. of 75 patients examined, 11 demonstrated failing fixtures. 22 of 48 fixtures failed in 6 fully edentulous patients and 10 of 34 failed in 5 partially edentulous patients. the etiology of failure was attributed to infection if there was bleeding, suppuration, pain, high plaque and gingival indices and granulomatous tissue upon surgical removal. traumatic etiology was suspected in ...19911843467
anaerobic pathogenesis: collagenase production by peptostreptococcus magnus and its relationship to site of infection.fifty isolates of peptostreptococcus magnus from intraabdominal sepsis, nonpuerperal breast abscess, and diabetic foot infections were examined for collagenase activity using bovine type i collagen. collagenase production was detected in a higher percentage of strains from nonpuerperal breast and diabetic foot specimens (p less than .001). this enzyme may be responsible for p. magnus playing a more central role in the pathogenesis of nonpuerperal breast abscess and diabetic foot disease than in ...19911850443
bacteriological investigations of clinical mastitis in heifers in sweden.bacterial analyses were carried out of 2069 udder secretions, isolated from 1481 heifers with mastitis in eight veterinary districts in sweden. streptococci, e.g. streptococcus dysgalactiae and str. uberis, dominated the bacterial flora, being isolated from 34.4 and 19.5% respectively of heifers with clinical mastitis occurring from puberty up to 14 d post partum. bacterial species generally regarded as important pathogens in the summer mastitis complex, e.g. actinomyces pyogenes, stuart-schwan ...19911856352
antibiosis between bacteria isolated from the vagina of women with and without signs of bacterial vaginosis.lactobacilli from women with and without bacterial vaginosis (bv) were tested for h2o2 production. thirty-seven (79%) of the 47 strains of lactobacilli isolated from the women without bv produced h2o2, while only nine (23%) of the 39 strains of lactobacilli obtained from women with bv did so. five of 20 h2o2-producing and two of 26 non-producing strains of lactobacillus exhibited antibiosis against four of 12 strains of peptostreptococci and two of 10 strains of mobiluncus spp. none of a further ...19911859646
nosocomial infections of ocular conjunctiva in newborns delivered by cesarian section.colonization of the ocular conjunctiva in newborns delivered by cesarian section occurs usually within the first day of life. we have studied the flora of the ocular conjunctiva at birth, from 19 newborns delivered by cesarian section, coming from two different maternity hospitals. ocular conjunctiva cultures yielded the main predominant flora in both maternity hospitals considered. the most common genus of this flora are: staphylococcus, corynebacterium and propionibacterium acnes. peptostrepto ...19911870845
[clinical study on cefteram pivoxil in asymptomatic bacteriospermia].in order to understand the clinical efficacy of cefteram pivoxil (cftm) in the treatment of asymptomatic bacteriospermia, the following studies were performed. 1. concentrations of cftm in the semen after oral administration of 200 mg to normal healthy adults (n = 5) reached a maximum level of 0.66 +/- 0.04 microgram/ml in 5 hours after the administration, then decreased rapidly, and averaged 0.15 +/- 0.03 micrograms at 7 hours after administration. 2. activities of cftm, cefaclor (ccl) and lome ...19911880933
[randomized study of amoxicillin-clavulanic acid versus amoxicillin-metronidazole combinations in pyogenic infections of dental origin].thirty patients, without any valve defect, cardiac malformation or previous antibiotic administration and presenting dental infections, have been treated either with amoxycillin-clavulanic acid or amoxycillin and metronidazole. aerobic and anaerobic bacteria have been isolated. these infections were due to mixed flora. nearly one half of anaerobes were peptostreptococcus, s. milleri and s. sanguis being predominant among aerobes. the species identified were those found in the mouth in the absenc ...19911881695
[diagnosis of contact mediastinitis in necrotic phlegmons of the neck]. 19911882299
the importance of positive bacterial cultures of specimens obtained during clean orthopaedic operations.microbiological cultures of specimens of tissue and of fluids from the wound in forty patients who had had consecutive clean, elective orthopaedic operations (excluding total joint replacements) and had not received antibiotics preoperatively were analyzed. of the forty patients, twenty-three (58 per cent) had a positive culture on at least one of the media that were used and seventeen (43 per cent) had negative cultures. of the forty specimens that were obtained from swabbing of the wound, eigh ...19911890121
chronic conjunctivitis caused by oral anaerobes and effectively treated with systemic metronidazole plus this study, we report on a case of refractory, unilateral anaerobic conjunctivitis. the predominant anaerobic flora consisted of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. by using the technique of restriction endonuclease fingerprinting of genomic dna, it was shown that the p. intermedia likely originated from the oral cavity. topically applied antibiotics had failed to suppress the infection in the past. successful treatment was achieved after sy ...19911890173
[2 cases of rhinogenic intracranial complications with normal rhinoscopy].two cases of non-postoperative rhinogenic intracranial complications are reported: patient 1 was a 25-year-old female with meningitis and right orbital phlegmon, and patient 2 was a 21-year-old male with a brain abscess. main symptoms in these patients were meningeal signs and high fever, and intranasal findings were normal in both patients. the diagnosis was not made until computed tomography revealed paranasal abnormalities. soon after surgical intervention and treatment with large doses of an ...19911896694
purification and characterization of nadp(+)-dependent 5,10-methylenetetrahydrofolate dehydrogenase from peptostreptococcus productus marburg.the 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown peptostreptococcus productus marburg was purified to apparent homogeneity. the purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with nadp+ as the electron acceptor at a specific activity of 627 u/mg of protein. the km values for methylenetetrahydrofolate and for nadp+ were 27 and 113 microm, respectively. the enzyme, which lacked 5,10-methenyltetrahydrofolate cyclohydrolase activity, was in ...19911899860
microbial aspects of failing osseointegrated dental implants in humans.the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ...19911906537
[hemolytic jaundice in anaerobic bacteremia. a report of a case resolved surgically].the authors report a case of haemolytic jaundice caused by anaerobic bacteria arising from an abdominal abscess. this is undoubtedly a rare pathology but, when occurring, the resolution may be achieved by surgical therapy.19911911074
suitability of the broth-disk elution test for evaluating susceptibility of obligate anaerobes to trimethoprim-sulfonamides.species of anaerobic bacteria isolated from clinical veterinary specimens were tested for susceptibility to trimethoprim-sulfonamides by the broth-disk elution technique. three different media were used for each organism: prereduced anaerobically sterilized (pras) brain-heart infusion broth (bhi), thioglycollate broth, and a semidefined pras medium. susceptibility results from these media were compared with those determined by interpreting the minimal inhibitory concentration obtained using an a ...19911911991
bacteriologic monitoring of periodontal diseases: cultural, enzymatic, immunologic, and nucleic acid studies.evidence supporting the bacteriologic etiology of periodontal diseases has led investigators to monitor the microbiologic species associated with each disease. the type of study performed has evolved at different levels over the years and is predominantly influenced by available technologies. as the technology has advanced, so too have attempts at monitoring specific microorganisms. the ultimate goal in most research efforts on clinical bacteriologic monitoring of the periodontal diseases has be ...19911912631
protein-kinase-c-independent activation of arachidonate release and prostaglandin e2 formation in macrophages interacting with certain bacteria.certain bacterial species, of which we selected fusobacterium nucleatum, gardnerella vaginalis, peptostreptococcus anaerobius and propionibacterium acnes, were found to induce release of arachidonic acid in a dose- and time-dependent manner in mouse macrophages. the release of arachidonic acid showed a characteristic lag period of approximately 10 min and was accompanied by selective transformation into prostaglandin e2. bacteria killed by various methods caused a similar response, indicating th ...19911915341
selection, expression, and nucleotide sequencing of the glutamate dehydrogenase gene of peptostreptococcus asaccharolyticus.the gene for the catabolic nad-linked glutamate dehydrogenase of peptostreptococcus asaccharolyticus was cloned by selection of escherichia coli for complementation of a biosynthetic defect. cloned fragments containing the gene and the p. asaccharolyticus transcription and translation signals are very highly expressed in e. coli. the nucleotide sequence of the cloned gene was determined. it codes for a polypeptide of 421 amino acids, the sequence of which is similar to those of the nadp-acceptin ...19911917850
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