normal bacterial flora from vaginas of criollo limonero order to describe the normal bacterial flora in vaginas of criollo limonero cows, 51 healthy multiparous cows, at least 90-day postpartum, were selected. duplicated swabs (n = 102) were taken from the vaginal fornix of cows to perform aerobic and anaerobic cultures as well as conventional biochemical tests. out of 102 swabs, bacterial growth was obtained in 55 (53.9%) while the remaining 47 (46.1%) did not exhibited any bacterial growth. of the 55 bacterial growths, 23 (41.8%) were aerobic wh ...201021082249
brainstem abscess caused by haemophilus influenza and peptostreptococcus species.we present a patient with a solitary brainstem abscess caused by haemophilus influenza type b (hib) and peptostreptococcus species. this is the first report of a hib brainstem abscess in the english literature. hib has been mainly associated with respiratory or nasal infections, and a few cases of intracerebral abscesses, but no brainstem abscesses have been described. the literature on solitary brainstem abscesses was reviewed and an overview of the literature between january 1984 and may 2009 ...201021109442
clinico-microbiological study and antimicrobial drug resistance profile of diabetic foot infections in north india.the study was carried out in diabetic patients with foot ulcer to determine the microbiological profile of infected ulcer, antibiotic resistance of the isolates and to find out the potential risk factors for infection with multidrug resistance and the outcome of these infections. a detailed clinical history and physical examination was carried out in each patient. pus samples for bacterial culture were collected from 102 patients admitted with diabetic foot infections. all patients had ulcer wit ...201021130641
bacteriologic culture of excised intervertebral disc from immunocompetent patients undergoing single level primary lumbar design: a consecutive case series from a single center of patients undergoing primary microdiscectomy for lumbar herniated nucleus pulposus (hnp) who received microbiologic laboratory culture of excised disc material. objective: to determine the prevalence of positive bacterial cultures in the disc material of immunocompetent patients without diabetes mellitus or other immune compromise. summary of background data: the intradiscal space is a physiologically tenuous environment in terms of ...201021150662
comparative evaluation of bactericidal potential of four root canal filling materials against microflora of infected non-vital primary teeth.since complete debridement of the root canals of the primary teeth is not practically possible due to the highly variable root canal anatomy, success of the endodontic therapy depends partly on the use ofantibacterial irrigating agents and root canal filling materials. recent literature indicates that anaerobes comprise a majority of the bacteria in necrotic root canals ofprimary teeth. the study determined the antibacterial effectiveness of four root canal filling materials namely calcium hydro ...201021189760
necrotising pneumonia due to finegoldia magna. 201021193258
efficacy of an anaerobic swab transport system to maintain aerobic and anaerobic microorganism viability after storage at -80 degrees amies agar gel swab transport system was evaluated for its ability to maintain bacterial viability and relative quantity after freezing at -80°c. nine american type culture collection (atcc) bacterial strains were used: 3 anaerobic strains (propionibacterium acnes, peptostreptococcus anaerobius, and clostridium sporogenes) and 6 facultative or strict aerobic bacterial strains (stenotrophomonas maltophilia, escherichia coli ([atcc 25922 and atcc 11775], salmonella enterica subsp. enterica sero ...201121217035
antimicrobial activity of diterpenes from viguiera arenaria against endodontic bacteria.six pimarane-type diterpenes isolated from viguiera arenaria baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. the microdilution method was used for the determination of the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) against porphyromonas gingivalis, prevotella nigrescens, prevotella intermedia, prevotella buccae, fusobacterium nucleatum, bacteroi ...201121233793
fluorescence correlation spectroscopy of fast chain dynamics within denatured protein l.intramolecular dynamics in the denatured state of a protein are of importance for protein folding. native-like contact formation within the ensemble of denatured conformations of a protein may guide its transformation towards the native conformation. the efficiency of folding is thus dependent on the diffusion of chain fragments, which facilitates contact formation. herein we investigate intramolecular diffusion of denatured molecules of the small two-state-folding protein l with fluorescence co ...201121271633
microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis.the objective was to examine the use of a tailor-made dna microarray containing probes representing the vaginal microbiota to examine bacterial vaginosis.201121272848
evaluation of three swab transport systems for the maintenance of clinically important bacteria in simulated mono- and polymicrobial this study, three swab transport systems were evaluated: m40 transystem, amies broth with a relatively new type of swab (both copan diagnostics, corona, ca, usa), and ssi transportmedium (statens serum institut, copenhagen denmark). the clsi m40-a standard procedures and 11 culture collection strains were used. the transport systems were tested at room temperature for holding times of 0, 24, and 48 h, and both mono- and polymicrobial samples were included. after 24 h of simulated transportati ...201121284737
an in vitro comparative study determining bactericidal activity of stabilized chlorine dioxide and other oral rinses.the study was conducted to determine the bactericidal activity of a stabilized chlorine dioxide oral rinse (closys oral rinse) compared to products currently available on the market.201121290979
impact of systemic antimicrobials combined with anti-infective mechanical debridement on the microbiota of generalized aggressive periodontitis: a 6-month compare the effects of systemic amoxicillin (amx) plus metronidazole (met) or placebos combined with anti-infective mechanical debridement on the sub-gingival microbiota of generalized aggressive periodontitis (gap).201121303403
salivary blood group antigens and microbial flora. 201021356009
microbiology of sinusitis.most sinus infections are viral, and only a small proportion develops a secondary bacterial infection. rhinoviruses, influenza viruses, and parainfluenza viruses are the most common causes of sinusitis. the most common bacteria isolated from pediatric and adult patients with community-acquired acute purulent sinusitis are streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, and streptococcus pyogenes. staphylococcus aureus and anaerobic bacteria (prevotella and porphyromonas, ...201121364226
tongue biofilm areal density and tongue coating index.two methods have been employed to measure the amount of tongue biofilm in humans: (1) areal density of microbes (cfu cm(-2)) (tongue scrape from a known/measured area), or (2) a tongue-coating index, where a human judge assesses the amount of 'coating' by visual inspection. the two methods were compared, both on human volunteers and on artificial biofilms. fusobacterium nucleatum (fn), prevotella intermedia (pi), streptococcus mutans (sm) and peptostreptococcus micros (pm) were obtained from sto ...200821386152
antibiotic susceptibility of cocultures in polymicrobial infections such as peri-implantitis or periodontitis: an in vitro model.background: although polymicrobial infections, such as peri-implantitis or periodontitis, were postulated in the literature to be caused by synergistic effects of bacteria, these effects remain unclear looking at antibiotic susceptibility. the aim of this study is to compare the antibiotic susceptibilities of pure cultures and definite cocultures. methods: laboratory strains of aggregatibacter actinomycetemcomitans (aa) (previously actinobacillus actinomycetemcomitans), capnocytophaga ochracea ( ...201121405933
clinical periodontal and microbiologic parameters in patients with rheumatoid arthritis.background: a limited number of studies suggest the prevalence of periodontal pathogens in patients with rheumatoid arthritis (ra); however, results are inconsistent. the aim of this study was to investigate clinical periodontal and microbiologic parameters in patients with ra. methods: 66 patients with ra aged 49.5±8.4 years participated in the study. the periodontal situation was assessed with the periodontal screening index (psr(®)/psi); allocated to: healthy, gingivitis (psr(®)/psi score 0-2 ...201121405936
ovine pedomics: the first study of the ovine foot 16s rrna-based microbiome.we report the first study of the bacterial microbiome of ovine interdigital skin based on 16s rrna by pyrosequencing and conventional cloning with sanger-sequencing. three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. the sheep were classified as having either healthy interdigital skin (h) and interdigital dermatitis (id) or virulent footrot ...201121430786
[bacterial mixed infection in women with chronic recurrent cystitis].to study microbial repertoire of urine in healthy women and patients with chronic recurrent cystitis (crc) including facultative anaerobic (fa) and non-clostridial anaerobic (nca) bacteria. materials and methods. triple bacteriological study of urine was performed in three groups of women: group i--22 healthy virgin women aged 18- 25 years, group ii--24 women aged 18 - 25 years with regular sexual contacts, group iii--72 women aged 20 - 60 years with crc, before antibacterial therapy. bacteriolo ...201121446162
perioperative, postoperative, and prophylactic use of antibiotics in alloplastic total temporomandibular joint replacement surgery: a survey and preliminary guidelines.purpose: in 2009, the american academy of orthopedic surgeons recommended lifelong prophylaxis after orthopedic total joint replacement (tjr) before these patients undergo dental, aerodigestive, genitourinary (gu), and gastrointestinal (gi) procedures. because oral and maxillofacial surgeons worldwide are implanting alloplastic total temporomandibular joint replacements (tmj tjrs), it appeared reasonable to survey these surgeons to obtain data that might shed some light, not only on this issue, ...201121470752
bacteriological findings and antimicrobial resistance in odontogenic and non-odontogenic chronic maxillary sinusitis.the main objectives of this study were to estimate the frequency of chronic maxillary sinusitis of dental origin, and to evaluate the microbiology of odontogenic and non-odontogenic chronic maxillary sinusitis. aspirates of 59 patients with chronic maxillary sinusitis (47 non-odontogenic, 12 odontogenic), collected during a 3-year period, were microbiologically processed for aerobic and anaerobic bacteria. moreover, in the isolated bacteria the antimicrobial susceptibility was evaluated. in this ...201121498651
gingival tissue transcriptomes in experimental gingivitis.jönsson d, ramberg p, demmer rt, kebschull m, dahlén g, papapanou pn. gingival tissue transcriptomes in experimental gingivitis. j clin periodontol 2011; doi: 10.1111/j.1600-051x.2011.01719.x. abstract: aims: we investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis. material and methods: twenty periodontally and systemically healthy non-smoking volunteers participated in a 3-week experimental gingivitis protocol, followed by deb ...201121501207
sitafloxacin: in bacterial infections.sitafloxacin is a fluoroquinolone antibacterial with in vitro activity against a broad range of gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical pathogens. it is approved in japan for use in a number of bacterial infections caused by sitafloxacin-susceptible strains of staphylococcus spp., streptococcus pneumoniae, other streptococcus spp., enterococcus spp., moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., enterobacter sp ...201121504249
[resistance to b-lactamines of bacteria responsible for endodontic root canal infections].samples were taken under strictly anaerobic conditions from the root canals of 22 patients having a tooth in which necrotic pulp was associated with an inflammatory peri-apical lesion an optical periodontitis. in the majority of cases (85%), these lesions were chronic and the pulp chamber was closed. one hundred and two strains were isolated, 71.7% of the bacteria being obligate anaerobes and 49.8% being gram-negative bacilli. their ability to produce beta-lactamases was tested and 8.8% gave a p ...201021510355
microflora in maxillofacial infections-a changing scenario?purpose: advances in isolation and culturing techniques have brought the role of anaerobic bacteria-causing maxillofacial infections to the fore. recent literature also favors the role of anaerobes in maxillofacial infections. a prospective clinical and microbiological study was designed to check the validity of such claims. patients and methods: this study included 88 patients who fulfilled the inclusion criteria. pus was obtained by aspirating the involved spaces and culture and sensitivity te ...201121511379
bacterial diversity in persistent periapical lesions on root-filled teeth.the purpose of this study was to analyze the bacterial diversity in persistent apical lesions on root-filled teeth by using culture-independent molecular methods.200921523213
analysis of microbiota associated with peri-implantitis using 16s rrna gene clone library.peri-implantitis (pi) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. the subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in pi, the microbiota responsible for pi has not been identified.201021523229
osteoradionecrosis contains a wide variety of cultivable and non-cultivable microscopy, anaerobic culture and dna-dna hybridization have previously demonstrated an association between microorganisms and osteoradionecrosis (orn). the purpose of our study was to use culture independent molecular techniques to detect bacteria in necrotic bone lesions of the mandible after radiation therapy.201021523230
analyses of recombinant stereotypic ighv3-21-encoded antibodies expressed in chronic lymphocytic leukemia.chronic lymphocytic leukemia (cll) cells that use igh encoded by ighv3-21 and that have a particular stereotypic third cdr (hcdr3), dangmdv (motif-1), almost invariably express ig l chains (igl) encoded by iglv3-21, whereas cll that use ighv3-21-encoded igh with another stereotypic hcdr3, dpsfyssswtlfdy (motif-2), invariably express κ-igl encoded by igkv3-20. this nonstochastic pairing could reflect steric factors that preclude these igh from pairing with other igl or selection for an ig with a ...201121525382
macromolecular crowding fails to fold a globular protein in cells.proteins perform their functions in cells where macromolecular solutes reach concentrations of >300 g/l and occupy >30% of the volume. the volume excluded by these macromolecules stabilizes globular proteins because the native state occupies less space than the denatured state. theory predicts that crowding can increase the ratio of folded to unfolded protein by a factor of 100, amounting to 3 kcal/mol of stabilization at room temperature. we tested the idea that volume exclusion dominates the c ...201121534566
effects of adding polyclonal antibody preparations on ruminal fermentation patterns and digestibility of cows fed different energy sources.nine ruminally cannulated cows fed different energy sources were used to evaluate an avian-derived polyclonal antibody preparation (pap-mv) against the specific ruminal bacteria streptococcus bovis, fusobacterium necrophorum, clostridium aminophilum, peptostreptococcus anaerobius and clostridium sticklandii and monensin (mon) on ruminal fermentation patterns and in vivo digestibility. the experimental design was three latin squares 3 x 3 distinguished by the main energy source in the diet [dry-g ...201121551346
detection of nine microorganisms from the initial carious root lesions using a taqman-based real-time pcr.oral diseases (2011) objective:  the purpose of this study was to quantify nine selected cariogenic bacteria in plaque from sound root surfaces and initial carious root lesions using taqman pcr and to analyse a putative dependence on the kind of initial periodontal treatment. material and methods:  fifty-four subjects with generalized chronic periodontitis were randomly allocated to one of the following initial periodontal therapies: full-mouth disinfection, full-mouth scaling and root planing o ...201121605286
recovery of aerobic and anaerobic bacteria in sinus fungal ball.this case study with chart review describes the aerobic and anaerobic microbiology of specimens obtained from 47 patients with aspergillus spp fungus ball. bacteria were recovered from 32 of the 47 (68%) patients. eighty-six isolates, 29 aerobic and facultatives and 57 anaerobic, were recovered. aerobic and facultatives only were recovered in 6 instances (19% of culture-positive specimens), anaerobes only in 11 (34%), and mixed aerobes and anaerobes in 15 (47%). the predominant aerobes were stap ...201121804027
analysis of the antimicrobial susceptibility of anaerobic bacteria isolated from endodontic infections in brazil during a period of nine years.the purpose of this study was to analyze the susceptibility of some anaerobic species isolated from a brazilian population at different periods of time by determining a pattern of development of resistance to frequently prescribed antibiotics in endodontics.201121763894
appendicitis: when there is more than meets the eye.acute appendicitis is the most frequent emergency in gastrointestinal surgery. obstruction of the appendiceal lumen appears to be one of the most common physiologic mechanisms for the development of acute appendicitis. once obstructed, the dilatation of the lumen causes ischemia and necrosis of the wall. the most common organisms involved in appendicitis are escherichia coli, peptostreptococcus, bacillus fragilis and pseudomonas. rarely, actinomyces is involved in this process. in this case repo ...201121763232
anaerobic bacteria colonizing the lower airways in lung cancer patients.anaerobes comprise most of the endogenous oropharyngeal microflora, and can cause infections of airways in lung cancer patients who are at high risk for respiratory tract infections. the aim of this study was to determine the frequency and species diversity of anaerobes in specimens from the lower airways of lung cancer patients. sensitivity of the isolates to conventional antimicrobial agents used in anaerobe therapy was assessed. respiratory secretions obtained by bronchoscopy from 30 lung can ...201121744326
[characteristics of microbiocenosis of gingival pocket in patients with chronic generalized periodontitis].as a result of examination of 56 patients with chronic generalized periodontitis (cgp) of a moderate severity and a severe form of the disease, microflora from gingival pocket in patients which severe form of cgp was established to have higher specific weight in index of dissemination of strains such as staphylococcus aureus, streptococcus pyogencs, enterococcus, corynebacterium spp., anaerobic bacteria. strains from patients with a severe form of cgp were characterized by a higher level of expr ...201121716238
deep neck abscess: an analysis of microbial etiology and the effectiveness of antibiotics.the objective was to demonstrate the aerobic and anaerobic microbiology of deep neck space abscess and to analyze the coverage rate of different empiric antimicrobial agents. a retrospective review of hospitalized patients with deep neck abscess diagnosed at a tertiary-care, general hospital between april 2001 and october 2006. the study enrolled 100 patients. the bacterial cultures of 89 patients yielded positive results (89%). the predominant aerobes were viridans streptococci, klebsiella pneu ...200821694873
sudden onset proptosis secondary to cavernous sinus thrombosis from underlying mandibular dental infection.the present report concerns a patient who presented with a 4-day history of left-sided facial pain arising from a pre-existing dental infection and progressive shortness of breath. the patient had a previous diagnosis of rheumatoid arthritis and was being treated with methotrexate. the rapid development of a right eye proptosis necessitated urgent decompression with a lateral canthotomy and cantholysis. imaging revealed a left facial abscess, cavernous sinus thrombosis (cst), bilateral internal ...200921789100
enteral tube feeding alters the oral indigenous microbiota in elderly adults.enteral tube feeding is widely used to maintain nutrition for elderly adults with eating difficulties, but its long-term use alters the environment of the oral ecosystem. this study characterized the tongue microbiota of tube-fed elderly adults by analyzing the 16s rrna gene. the terminal restriction fragment length polymorphism (t-rflp) profiles of 44 tube-fed subjects were compared with those of 54 subjects fed orally (average age 86.4 -¦ 6.9 years). barcoded pyrosequencing data were also obta ...201121821752
peptostreptococcus anaerobius infective endocarditis complicated by spleen infarction.peptostreptococcus sp., which are normal flora of human mucocutaneous surfaces, can be recovered in mixed infections involving the skin, soft tissue, respiratory tract, gastrointestinal tract and genitourinary tract. peptostreptococci are rarely reported to be the cause of infective endocarditis. because of their fastidiousness, peptostreptococci are difficult to isolate and are often overlooked. the authors report a case of peptostreptococcus infective endocarditis of a native mitral valve comp ...201121709536
Dental Abnormalities in Eight Captive Giant Pandas (Ailuropodamelanoleuca) in China.Dental data from eight adult giant pandas (Ailuropoda melanoleuca) (four females and four males) were collected at the Beijing Zoo from February 2009 to July 2010. Examination findings were recorded in dental charts. All the pandas had different degrees of tooth wear. Incisors, canines and second premolars had the most abnormalities. Five animals had caries; molars were the most affected. Chip fractures were found in seven teeth (incisor, canine and premolar) of five pandas; two had complicated ...201121906751
Endodontic pathogens causing deep neck space infections: clinical impact of different sampling techniques and antibiotic susceptibility.The aims of the present study were to compare microbial populations in patients suffering from deep neck space abscesses caused by primary endodontic infections by sampling the infections with aspiration or swabbing techniques and to determine the susceptibility rates of the isolated bacteria to commonly used antibiotics.201121846534
biomimetic affinity ligands for immunoglobulins based on the multicomponent ugi reaction.affinity chromatography is the method of choice for biomolecule separation and isolation with highly specific target recognition; it is ideally suited to the purification of immunotherapeutic proteins (i.e., mabs). conventional affinity purification protocols are based on natural immunoglobulin (ig)-binding proteins, which are expensive to produce, labile, unstable, and exhibit lot-to-lot variability. biological ligands are now being replaced by cost-effective, synthetic ligands, derived from th ...201221964782
plasma half-life extension of small recombinant antibodies by fusion to immunoglobulin-binding domains (igbd).many therapeutic proteins possessing a small size are rapidly cleared from circulation. half-life extension strategies have therefore become increasingly important to improve the pharmacokinetic and pharmacodynamic properties of protein therapeutics. here, we performed a comparative analysis of the half-life extension properties of various bacterial immunoglobulin-binding domains (igbd) derived from staphylococcus protein a (spa), streptococcus protein g (spg) and finegoldia (formerly peptostrep ...201122147690
management outcome of npc-related and non-npc-related brain abscess in hong kong.objectives: (1) to review the patient profile, management outcome and prognostic factors of brain abscess; (2) to compare the neurological outcome of nasopharyngeal carcinoma (npc)-related brain abscess with non-npc related brain abscess. method: retrospective review of consecutive patients diagnosed (radiologically and/or microbiologically) with brain abscess in a regional neurosurgical center in hong kong over a nine year period. results: fifty-four patients were recruited into this study. the ...201122178290
[clinical effect of chitosan in bacterial vaginosis therapy].determination of antibacterial activity of acid-soluble chitosan in bacterial vaginosis therapy in women of reproductive age and comparison of therapy results of traditional scheme and local administration of acid-soluble chitosan.201122145355
fournier's gangrene in elderly patient: report of a case.fournier's gangrene (fg) is rapidly progressing acute gangrenous infection of the anorectal and urogenital area. fg needs precocious diagnosis and aggressive treatment with the use of wide spectrum antibioticus and surgical debridement. in our case, a 91-year-old japanese female who had rehabilitation after treatment of pneumonia and her past history was rheumatoid arthritis treated with steroid and chronic heart failure. her activities of daily living was bedridden with dementia. necrotic skin ...201121921428
cervical necrotizing fasciitis and a descending mediastinal abscess caused by acute epiglottitis with diabetes mellitus: a life-threatening complication.cervical necrotizing fasciitis and a descending mediastinal abscess of the neck following acute epiglottitis, life-threatening complications, is reported in a 43-year-old man with dm. the bacterial culture showed peptostreptococcus anaerobius. the patient recovered after surgical debridement and broad-spectrum antibiotics therapy.201122088790
[repression of bacterial biofilms in suppurative necrotic complications of diabetic foot syndrome by ultrasound cavitation].study of wound microbial landscape in suppurative necrotic complications in patients with diabetic foot syndrome (dfs), and a possibility of suppression of bacterial biofilm development in these wounds by ultrasound cavitation (usc).201121913385
structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers.despite a long-suspected role in the development of human colorectal cancer (crc), the composition of gut microbiota in crc patients has not been adequately described. in this study, fecal bacterial diversity in crc patients (n=46) and healthy volunteers (n=56) were profiled by 454 pyrosequencing of the v3 region of the 16s ribosomal rna gene. both principal component analysis and unifrac analysis showed structural segregation between the two populations. forty-eight operational taxonomic units ...201121850056
[bacteriology of ethmoidal sinus in chronic rhinosinusitis with and without nasal polyps].to compare the bacteriologic features of anterior ethmoidal biopsy specimens between chronic rhinosinusitis without nasal polyps (crsnp-), chronic rhinosinusitis with nasal polyps(crsnp+) and control patients.201121845823
vulvar abscess: a retrospective review.vulvar abscesses can progress to cause significant illness requiring disfiguring surgical debridement, broad spectrum antibiotics, and require hospitalization. a retrospective review of de-identified charts of 13 patients admitted for inpatient care for vulvar abscesses from 2004-2009 at west virginia university hospitals was conducted. risk factors for vulvar abscess in these patients included obesity and diabetes. body mass index (bmi) is directly proportionate to the size of the abscess and i ...201021932487
Primary sternal osteomyelitis due to Peptostreptococcus anaerobius.Primary sternal osteomyelitis (PSO) is a rare syndrome. In adults, it usually occurs with underlying predisposing factors, such as immunodeficiency, or intravenous (IV) drug abuse. The infecting organism in these patients is usually Staphylococcus aureus or Pseudomonas aeruginosa. Peptostreptococcus species are Gram-positive anaerobic cocci and are part of the normal flora of human mucocutaneous surfaces. Peptostreptococcus infection can occur in all body sites, including the central nervous sys ...201121847552
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