anaerobic culture of diabetic foot infections: organisms and antimicrobial susceptibilities.the prevalence of diabetes mellitus is high in singapore. infections of the lower limb are significant causes of morbidity in this population. although the aerobic bacteriology of these infections is well-documented, there is less data available on the anaerobic pathogens involved. this study sets out to describe the anaerobic bacteria associated with diabetic foot infections, and evaluates the susceptibility to 3 antimicrobials with anaerobic activity.200819082200
[antibiotic therapy in odontogenic infections in children and adolescents: pharmacokinetic/pharmacodynamic analysis].the objective of this study was to evaluate the efficacy of the most commonly used antimicrobial treatments in odontogenic infections in children and adolescents on the basis of pharmacokinetic/ pharmacodynamic (pk/pd) criteria.200819100192
the role of anaerobic bacteria in chronic suppurative otitis media in children: implications for medical therapy.this review describes the microbiology, diagnosis and medical management of chronic suppurative otitis media (csom) in children highlighting the role of anaerobic bacteria. in studies that employed adequate method for recovery of anaerobic bacteria polymicrobial aerobic and anaerobic flora was isolated from over half of the children with csom. the predominant aerobic isolates were staphylococcus aureus and pseudomonas aeruginosa and the most frequently isolated anaerobic organisms were peptostre ...200819114112
two teenagers with sepsis and pneumonia. 200919114923
effectiveness of ozone against endodontopathogenic microorganisms in a root canal biofilm assess the antimicrobial efficacy of aqueous (1.25-20 microg ml(-1)) and gaseous ozone (1-53 g m(-3)) as an alternative antiseptic against endodontic pathogens in suspension and a biofilm model.200919125975
the microbiota of acute apical the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ...200919131319
sphenoid mucocele after radiation for the treatment of nasopharyngeal carcinoma. 200819133405
[comparative studying of anaerobic bacteria located in woman's reproductive ways in normal condition and dysbiosis].bacteriological investigation of obligate anaerobic bacteria located in ugt of two groups of the observed women has shown: that the microbiocoenosis of ugt of women of the group 1 can be determined as normal. identification of these anaerobic bacteria revealed the presence of representatives of the following species: lactobacillus spp., bifidobacterium spp., eubacterium spp., bacteroides spp., fusobacterium spp., peptococcus spp., peptostreptococcus spp. the microbiocoenosis of ugt of the women ...200819145822
the ability of non-bacteriocin producing streptococcus bovis strains to bind and transfer bovicin hc5 to other sensitive bacteria.streptococcus bovis hc5 produces a broad spectrum lantibiotic (bovicin hc5), but s. bovis jb1 does not have antimicrobial activity. preliminary experiments revealed an anomaly. when s. bovis jb1 cells were washed in stationary phase s. bovis hc5 cell-free culture supernatant, the s. bovis jb1 cells were subsequently able to inhibit hyper-ammonia producing ruminal bacteria (clostridium sticklandii, clostridium aminophilum and peptostreptococcus anaerobius). other non-bacteriocin producing s. bovi ...200919171197
lysozyme and microbiota in relation to gingivitis and periodontitis.the aim of this study was to investigate associations between the immune component of the oral cavity, lysozyme, in gingival crevicular fluid and unstimulated saliva, and colonization dynamics of periodontopathogenic bacteria from supra- and subgingival plaque samples in patients with gingivitis or periodontitis.200919179970
immunobiological activities of bacteria isolated from the root canals of postendodontic teeth with persistent periapical lesions.although intraradicular bacteria are widely considered to be the primary etiological agents of periapical lesions, the immunobiological properties of the species in question are not adequately known. the aim of this study, therefore, was to investigate the pathobiological properties of the 10 most frequently isolated endodontopathic bacterial species. using cellular components of the pathogens obtained by sonic extraction, we have investigated their ability to induce monocyte migration, interleu ...199219186419
antibiotic-resistant obligate anaerobes during exacerbations of cystic fibrosis patients.pseudomonas aeruginosa and staphylococcus aureus are thought to cause the majority of lung infections in patients with cystic fibrosis (cf). however, other bacterial pathogens may contribute to the pathophysiology of lung disease. here, obligate anaerobes were identified in a cross-sectional study, and cell numbers and antibiotic susceptibilities of facultative and obligate anaerobes from 114 sputum samples from nine children and 36 adults with cf were determined. furthermore, in 12 cf patients, ...200919196263
does diclofenac increase the risk of cervical necrotizing fasciitis in a rat model?non-steroidal anti-inflammatory drugs (nsaids) are known for aggravating in vitro infections and were reported in many cases of cervical necrotizing fasciitis (cnf). we developed a rat model of cnf, mimicking as closely as possible the human-cnf, to study the effect of a nsaids, diclofenac, as a promoting factor. twenty rats were injected bilaterally in the neck with peptostreptococcus and with a fresh saliva specimen for another 20 rats. half of each group was given an intramuscular injection o ...200919200252
microbial transformation and bioactivation of isoflavones from pueraria flowers by human intestinal bacterial strains.the flowers from pueraria, which are called puerariae flos, have been used since ancient times for recovery from alcohol intoxication. we elucidated the microbial transformation of the main isoflavones (1, 1a and 2) by using 29 commercially available human intestinal bacterial strains together with the bioactivation of the hepatoprotective activity of their metabolites. tectoridin (1a), which contains only one glucosyl moiety, was metabolized to its aglycone 1b by various bacterial strains. on t ...200919219523
the bacteriology of chronic rhinosinusitis with and without nasal compare the bacteriologic findings in ethmoidal biopsy specimens and nasal lavage samples from healthy control patients and from patients with chronic rhinosinusitis (crs) with nasal polyps (crsnp+) and without nasal polyps (crsnp-).200919221239
does pregnancy have an impact on the subgingival microbiota?we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.200919228092
[diabetic foot infections. prevalence and antibiotic sensitivity of the causative microorganisms].foot infections are a common reason for hospitalization and a cause of complications in patients with diabetes. the aim of this study was to determine the prevalence of microorganisms found on culture in complicated diabetic foot infections in hospitalized patients, and the sensitivity of the causative microorganisms to antimicrobial agents.200919237227
grafting of protein l-binding activity onto recombinant antibody fragments.recombinant antibody fragments consisting of variable domains can be easily produced in various host cells, but there is no universal system that can be used to purify and detect them in the free form or complexed with their antigen. protein l (ppl) is a cell wall protein isolated from peptostreptococcus magnus, which has been reported to interact with the v-kappa chain of some, but not all, antibodies. here we grafted the v-kappa framework region 1 (fr1) sequence of a high-affinity ppl-binding ...200919268418
clinical features and prognostic factors of anaerobic infections: a 7-year retrospective study.risk factors for mortality resulting from anaerobic infection are incompletely defined. the clinical significance of a broad range of pathogenic obligate anaerobic organisms was examined, and factors independently associated with mortality were identified in patients with clinically significant anaerobic infections.200919270476
into thin air. 200919272085
cytoprotective effect of proanthocyanidin-rich cranberry fraction against bacterial cell wall-mediated toxicity in macrophages and epithelial cells.recent studies brought evidence regarding the potential beneficial effects of cranberry polyphenols for periodontal infections. in this study, we evaluated the capacity of a proanthocyanidin-rich cranberry fraction to protect macrophages and oral epithelial cells against cytotoxicity induced by bacterial components. u937 cells, differentiated into adherent macrophage-like cells, as well as oral epithelial cells were treated with cell wall or lipopolysaccharide preparations from periodontopathoge ...200919277973
[septic shock caused by gardnerella vaginalis and peptostreptococcus species after cesarean section].a 41-year old multisectio patient got a life-threatening postoperative infection with gardnerella vaginalis and peptostreptococcus spp. perioperative treatment with cefuroxime and metronidazol is recommended.200919284925
anti-biofilm properties of satureja hortensis l. essential oil against periodontal pathogens.essential oils of several plants are widely used in ethnomedicine for their antimicrobial and anti-inflammatory properties. however, very limited data exist on their use in connection to periodontal diseases. the aim of the present study was to investigate the bacterial growth inhibiting and anti-biofilm effects of satureja hortensis l. (summer savory), salvia fruticosa m. (sage), lavandula stoechas l. (lavender), myrtus communis l., and juniperus communis l. (juniper) essential oils. chemical c ...200919285147
bacterial conversion of secoisolariciresinol and has been investigated whether secoisolariciresinol (seco) and anhydrosecoisolariciresinol (ahs), an acid degradation product of seco, could be fermented in a similar way, and to a similar extent, by members of the intestinal microbiota.200919302311
branchial cleft cysts in adults. diagnostic procedures and treatment in a series of 18 cases.branchial cleft anomalies may be presented as branchial cysts, fistulas, or sinuses. purpose of this paper is to present the diagnostic procedures and the treatment in a series of branchial cleft cysts.200919308468
[experimental vaginal dysbiosis on the model of white laboratory mice].qualitative and quantitative composition of microbiocenosis of urogenital tract (ugt) of mice has been studied. when investigating ugt of mice in norm (n = 8), microorganisms of several genera with following occurrence frequency were detected: lactobacillus (100%), streptococcus (100%), staphylococcus (87.5%), micrococcus (12.5%), bacillus (12.5%), fusobacterium (87.5%), peptococcus (62.5%), peptostreptococcus (50%), bacteroides (100%) and representatives of enterobacteriaceae f (12.5%) family. ...200819351049
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
meningitis and epidural abscess related to pansinusitis.sinusitis can rarely be latent and present directly with intracranial complications. we present the case of an 11-year-old girl who presented with typical features of meningitis. she underwent neuroimaging because of slow improvement and concern for a brain abscess. despite no history or examination findings suggestive of sinusitis, she was found to have pansinusitis with intracranial extension causing meningitis and epidural abscess.200919369843
surveillance and trends of antimicrobial resistance among clinical isolates of anaerobes in kuwait hospitals from 2002 to 2007.the susceptibility trends for all anaerobes processed by the anaerobe reference laboratory against various antibiotics were determined by using data for 2557 isolates referred by all government hospitals in kuwait from 2002 to 2007. mic were determined for the following anti-anaerobic antibiotics: amoxicillin-clavulanic acid, clindamycin, imipenem, meropenem, metronidazole, penicillin, piperacillin, piperacillin-tazobactam and vancomycin (for gram-positive anaerobes only), using e-test method. t ...201019406247
the antimicrobial effects of hops (humulus lupulus l.) on ruminal hyper ammonia-producing assess the antimicrobial effects of hops (humulus lupulus l.) on hyper ammonia producing-bacteria (hab), which catabolize amino acids and peptides in the bovine rumen.200919413813
mechanical characterization of protein l in the low-force regime by electromagnetic tweezers/evanescent nanometry.mechanical manipulation at the single molecule level of proteins exhibiting mechanical stability poses a technical challenge that has been almost exclusively approached by atomic force microscopy (afm) techniques. however, due to mechanical drift limitations, afm techniques are restricted to experimental recordings that last less than a minute in the high-force regime. here we demonstrate a novel combination of electromagnetic tweezers and evanescent nanometry that readily captures the forced un ...200919413987
the microbiota on different oral surfaces in healthy children.knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes.200919416446
ten-year follow-up of a patient with osler-weber-rendu syndrome and recurrent cerebral abscess secondary to pulmonary arteriovenous 1998, one of us (mjm) published an article discussing several patients with osler-weber-rendu syndrome or hereditary haemorrhagic telangiectasia with secondary cerebral complications. these were intracerebral haemorrhage, ischaemic infarct and intracerebral abscess. we outlined the background of the syndrome as well as its genotype and predicted an increasingly important role for genetic testing. our aim in this paper is to examine the progress of one of the patients as well as recommending f ...200919428258
the effects of treatment of acute otitis media with a low dose vs a high dose of amoxicillin on the nasopharyngeal compare the effects on the nasopharyngeal flora of therapy of acute otitis media in children with either a low dose or a high dose of amoxicillin.200919451466
fetal exposure to oral pathogens and subsequent risk for neonatal intensive care admission.maternal periodontal infection has been associated with adverse maternal and neonatal outcomes. in utero fetal exposure to oral pathogens was also recognized as deleterious to the fetus. the objective of this study was to determine the relationship between fetal exposure to oral pathogens and neonatal intensive care unit (nicu) admission.200919485816
[oral cavity microbiocenose in healthy teenagers and patients with chronic gastritis and gastroduodenitis].microbial association distinctive features and colonization degree of different oral cavity biotope were established in healthy teenagers and patients with chronic gastritis and gastroduodenitis. there were studied mucous membrane surfaces of cheek, tongue, content of gingival crevice, oral fluid and dental plaque of 24 healthy persons of the age 12-17 years and 29 patients of the same age with gastritis and gastroduodenitis. on the mucous membranes of the ill teenagers there were found porphiro ...200919491778
starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus.the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ...200919540450
descending necrotizing soft tissue infection of periodontal origin.descending necrotizing soft tissue infections involving the neck and chest are a rare but hazardous sequela of periodontal infections. these infections are associated with significant mortality.the high mortality rates of this condition are attributed to significant delays in diagnosis and treatment of common periodontal infections that have progressed to severe disease without overt clinical signs or symptoms. as with all necrotizing soft tissue infections, making a timely diagnosis requires th ...200919546678
comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples.200919563330
molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile.the microbial etiology of periradicular lesions is recognized; however, the bacterial profile of these lesions is not well elucidated. the purpose of this study was to determine the frequency of bacterial colonization in these lesions and to characterize the bacterial community present in root ends and periradicular lesions.200919567313
sudan azo dyes and para red degradation by prevalent bacteria of the human gastrointestinal tract.sudan azo dyes have genotoxic effects and ingestion of food products contaminated with sudan i, ii, iii, iv, and para red could lead to exposure in the human gastrointestinal tract. in this study, we examined thirty-five prevalent species of human intestinal bacteria to evaluate their capacity to degrade sudan dyes and para red. among these tested bacterial strains, 23, 13, 33, 30, and 29 out of 35 species tested were able to reduce sudan i, ii, iii, iv, and para red, respectively, to some exten ...201019580882
the bacteriology of salivary gland infections.the parotid gland is the salivary gland most commonly affected by inflammation. however, infection of the salivary glands can occur in any of the glands. the most common pathogens associated with acute bacterial infection are staphylococcus aureus and anaerobic bacteria. the predominant anaerobes include: anaerobic gram negative bacilli (eg, pigmented prevotella and porphyromonas); fusobacterium spp; and peptostreptococcus spp. in addition, streptococcus spp (including streptococcus pneumoniae) ...200919608044
effect of age on gingival crevicular fluid concentrations of mif and pge2.we used an experimental gingivitis study design to compare crevicular fluid concentrations of migration inhibitory factor (mif) and prostaglandin e(2) (pge(2)) in younger (18 to 30 yrs) and older (46 to 77 yrs) healthy adults. pge(2) increased after 1 wk in younger participants, whereas it decreased in older individuals after 1 wk of plaque accumulation. a significant interaction between age and time was observed for pge(2) (p = 0.04). high concentrations of mif were identified in both age group ...200919641151
bone microbial contamination influences autogenous grafting in sinus augmentation.the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation.200919656037
presence of aggregatibacter actinomycetemcomitans in young individuals: a 16-year clinical and microbiological follow-up look for clinical signs of periodontal disease in young adults who exhibited radiographic bone loss and detectable numbers of aggregatibacter actinomycetemcomitans in their primary dentition.200919678862
significance of cytomorphological and microbiological examination of bile collected by endoscopic cannulation of the papilla of vater.bile analysis yields important information such as "biliary microlithiasis" and biliary tract colonization by microorganisms like salmonella typhi or paratyphi, escherichia coli, etc., which may progresses to cholelithiasis and have been found to have a role in the development of gallbladder cancer in india.200919679953
identification of a mechanical rheostat in the hydrophobic core of protein l.the ability of proteins and their complexes to withstand or respond to mechanical stimuli is vital for cells to maintain their structural organisation, to relay external signals and to facilitate unfolding and remodelling. force spectroscopy using the atomic force microscope allows the behaviour of single protein molecules under an applied extension to be investigated and their mechanical strength to be quantified. protein l, a simple model protein, displays moderate mechanical strength and is t ...200919683005
[anaerobic bloodstream infections: study of 68 episodes].there is some controversy regarding the current rates of anaerobic bacteremia. some authors have described an increasing incidence in recent years, whereas others report declining rates. there is even debate over whether to routinely perform anaerobic blood cultures. we present a prospective analysis of anaerobic bloodstream infections diagnosed at our medical center from january 2003 to may 2008.201019683837
assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis.the aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (t-rflp), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features.200919702949
the use of pcr for the identification and characterisation of bacteriocin genes from bacterial strains isolated from rumen or caecal contents of cattle and sheep.pcr primers were designed to amplify the gene that encodes bovicin 255 from streptococcus gallolyticus lrc0255 and the bacteriocin genes from butyrivibrio fibrisolvens strains ar10 and or79a (bvid and bvi79a) in order to screen for their incidence in rumen and caecal b. fibrisolvens and streptococcus bovis-like isolates from new zealand and north american ruminants. none of the b. fibrisolvens-like strains (n=34) isolated from new zealand or north america had the genes encoding for butyrivibrioc ...200419712403
a case of brain abscess extended from deep fascial space infection.a case of brain abscess in the temporal lobe caused by direct intracranial extension of deep neck abscess is described. the abscess also spread to the orbital cavity through infraorbital fissure. the possible etiology of this case might be dental surgery. the diagnostic imaging clearly showed the routes of intracranial and -orbital extension of parapharyngeal and masticator space abscesses. from the abscess specimens, oral streptococci, anaerobic streptococci, and anaerobic gram-negative bacilli ...200919716487
comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim).200919722792
crystal structure of a mucus-binding protein repeat reveals an unexpected functional immunoglobulin binding activity.lactobacillus reuteri mucus-binding protein (mub) is a cell-surface protein that is involved in bacterial interaction with mucus and colonization of the digestive tract. the 353-kda mature protein is representative of a broadly important class of adhesins that have remained relatively poorly characterized due to their large size and highly modular nature. mub contains two different types of repeats (mub1 and mub2) present in six and eight copies, respectively, and shown to be responsible for the ...200919758995
abdominal pain after endoscopic hemostasis of gastric tumor bleeding. splenic infarction with abscess formation. 200919799837
comparative in-vitro activity of ceftazidime (gr-20263) and other beta-lactamase stable cephalosporins against anaerobic bacteria.the in-vitro activity of ceftazidime was determined against 586 clinical isolates of anaerobic bacteria by an agar-dilution technique and compared with that of cefuroxime, moxalactam and cefoxitin. at 16 mg/l, only 60% of all isolates were inhibited by ceftazidime, compared to 79% by cefuroxime, 81% by moxalactam and 80% by cefoxitin. ceftazidime was relatively inactive against bacteroides fragilis group, lactobacillus and clostridium species other than cl. perfringens. peptococcus, peptostrepto ...198119802974
postoperative mediastinitis due to finegoldia magna with negative blood cultures.we report a case of finegoldia magna (formerly known as peptostreptococcus magnus) mediastinitis following coronary artery bypass in a 50-year-old patient. even if staphylococci remain the main causative organism of postoperative mediastinitis, the responsibility of anaerobic bacteria must be considered in cases of fever and sternal drainage with negative blood cultures.200919812272
comparative evaluation of the antimicrobial efficacy of aloe vera tooth gel and two popular commercial toothpastes: an in vitro study.aloe vera (aloe barbadensis miller) has been suggested for a wide variety of ailments but its use in dentistry is limited. this article reviews the uses of the plant and describes an in vitro investigation that compared the antimicrobial effectiveness of aloe vera tooth gel with two popular, commercially available dentifrices. the preliminary results showed that aloe vera tooth gel and the toothpastes were equally effective against candida albicans, streptococcus mutans, lactobacillus acidophilu ...200919819812
fish bone-induced pancreatitis. 201019835985
maxillary sinusitis in patients ventilated for a severe head injury and with nostrils free of any foreign body.this study aims to determine the frequency of maxillary sinusitis in the patients with traumatic head injury and nostrils free of any foreign body. in addition, the sensitivity and specificity of ultrasonography (us) for the detection of the presence of fluid in maxillary sinuses were evaluated.201019879574
in vitro activity of tigecycline and comparators against a european compilation of anaerobes collected as part of the tigecycline evaluation and surveillance trial (test).the tigecycline evaluation and surveillance trial (test) is a global surveillance study designed to monitor the in vitro activity of the broad-spectrum antimicrobial tigecycline against nosocomial- and community-acquired pathogens. in this study the in vitro activity of tigecycline against 1256 anaerobic pathogens collected across europe has been compared to the activity of several comparator antibiotics. the pathogens examined in this study included bacteroides, prevotella, anaerococcus, clostr ...201019883155
a severely symptomatic case of anaerobic chronic bacterial prostatitis successfully resolved with moxifloxacin has been demonstrated that patients showing symptoms of chronic bacterial prostatitis but culture-negative prostate-specific specimens can benefit from administration of antibacterial agents. this suggests that organisms that are not isolated in the routine practice may be responsible for prostate infection in an undefined fraction of subjects. anaerobic bacteria have been proposed to play a pathogenic role in cbp, on the basis of studies describing clinical remission after eradication of pat ...201019883774
the role of anaerobic bacteria in bacteremia.anaerobic bacteria remain an important cause of bloodstream infections and account for 1-17% of positive blood cultures. this review summarizes the epidemiology, microbiology, predisposing conditions, and treatment of anaerobic bacteremia (ab) in newborns, children, adults and in patients undergoing dental procedures. the majority of ab are due to gram-negative bacilli, mostly bacteroides fragilis group. the other species causing ab include peptostreptococcus, clostridium spp., and fusobacterium ...201020025984
tissue plasminogen activator in the percutaneous drainage of splenic abscess. 201020036149
preliminary report on anaerobic culture at mymensingh medical college hospital in bangladesh.puerperal sepsis is an important cause of maternal morbidity and mortality in developing countries. this study was undertaken to isolate and identify the anaerobic bacterial agents of puerperal sepsis among the patients admitted in mymensingh medical college hospital (mmch) during the period from july 2006 to june 2007. endocervical swabs/secretions were collected from 50 cases of puerperal sepsis and were cultured anaerobically. anaerobiasis was done by using gas pack (bd gas pak tm ez) in anae ...201020046165
the bactericidal effect of a genius nd:yag evaluate the 'in vitro' bactericidal effect of the nd:yag laser (genius, mølsgaard dental, copenhagen, denmark) on six periodontal pathogens.201020096084
biomass-derived syngas fermentation into biofuels: opportunities and challenges.the conversion of biomass-derived synthesis gas (or syngas in brief) into biofuels by microbial catalysts (such as clostridium ljungdahlii, clostridium autoethanogenum, acetobacterium woodii, clostridium carboxidivorans and peptostreptococcus productus) has gained considerable attention as a promising alternative for biofuel production in the recent past. the utilization of the whole biomass, including lignin, irrespective of biomass quality, the elimination of complex pre-treatment steps and co ...201020096574
polymerase chain reaction-denaturing gradient gel electrophoresis, cloning, and sequence analysis of bacteria associated with acute periapical abscesses in children.polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), cloning, and sequencing were applied to the microbiologic study of acute periapical abscesses of endodontic origin in children to examine the predominant bacteria.201020113778
anterior chest wall abscess--an unusual complication of an odontogenic infection.introduction: a majority of odontogenic infections can be successfully managed with routine incision and drainage and empirical antibiotics. sometimes an odontogenic infection may spread to contiguous spaces and descend along the neck causing significant morbidity and rarely even mortality. case report: we report a case of an odontogenic infection which had an unusual spread to the anterior chest wall.201020135335
notes on the microbiology of cholesteatoma: clinical findings and treatment.anomalous proliferation of the cholesteatoma epithelium is caused by extrinsic factors such as toxins or bacterial antigens combined with lytic enzymes, lymphokines and cytokines released from the inflammatory infiltrate. this could explain the close relationship between the aggressiveness of cholesteatoma and repeated bacterial superinfection, therefore it is very important to know the bacteria involved in order to control the regrowth of skin following surgery, reduce the aggressive potential ...200920161877
development of a novel pcr method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections.the objective of this study was to develop a novel polymerase chain reaction (pcr) method to comprehensively analyze salivary bacterial flora.201020171911
orbital abscess associated with sinusitis from odontogenic origin. 201020190502
peptostreptococcus species: an unusual cause of infective endocarditis.peptostreptococcus species are part of the normal flora of human mucocutaneous surfaces. they account for 30% of anaerobes isolated from clinical sources, and are involved in a wide variety of clinically significant infections. peptostreptococcus magnus is the species most frequently isolated from clinical specimens as well as from normally sterile sites in pure culture. rarely have these organisms been reported as causes of endocarditis involving native and prosthetic valves, and very little is ...201020218045
spatial distribution of some microbial trophic groups in a plug-flow-type anaerobic bioreactor treating swine manure.anaerobic digestion of swine manure is carried out by a consortium of microbial species, including volatile fatty acid (vfa) producers, vfa-degraders and methanogens. the distribution of five phylogenetic groups within a plug-flow-type anaerobic bioreactor consisting of eight serially-connected tanks was examined through the sequential digestion of swine manure. quantification was carried out using reverse transcription real-time pcr (rt-q-pcr) assays targeting the 16s rrna of clostridium (clust ...201020220236
[acetate accumulation and shift of bacterial community during anaerobic sewage sludge fermentation by ph adjustment].to investigate the accumulation mechanism of volatile fatty acids (vfas) the shift of bacterial community and the contribution of syntroph acetogenic bacteria during the anaerobic fermentation of sewage sludge under different ph values.200920222451
photodynamic therapy in periodontal therapy: microbiological observations from a private recent years, the combination of laser light and photosensitizer known as photodynamic therapy (pdt) has been used in periodontal therapy. however, there are not enough clinical studies to fully evaluate the effects of pdt on the periodontal tissues. this microbiological study examined the effects of pdt on the periodontal bacteria in combination with scaling and root planing (srp) in the same group of patients by randomly selecting pdt or srp for use in different quadrants of the mouth. for ...201020236906
antibiotic susceptibility and resistance of the odontogenic microbiological spectrum and its clinical impact on severe deep space head and neck infections.the purpose of this retrospective study was to identify the major pathogens responsible for deep space head and neck infections and their current resistance to routinely used antibiotics in a university hospital setting.201020346713
peptostreptococcus infective endocarditis and bacteremia. analysis of cases at a tertiary medical center and review of the literature.peptostreptococcus infective endocarditis is rare but associated with high morbidity. we report two cases and evaluate all positive blood cultures for peptostreptococcus at our institution, followed by a review of the literature. this organism causes a subacute presentation and cardiac valve pathology is a risk factor. penicillin remains the treatment of choice.201020371296
a pediatric patient with acute suppurative thyroiditis caused by eikenella corrodens.a previously healthy 6-year-old boy had continuous fever for 6 days before admission to our hospital. his general condition was good except for pyrexia. the left lobe of the thyroid gland was swollen, red, hot, and tender, and neck movement was limited. the provisional diagnosis was upper respiratory tract infection. we demonstrated the existence of an orifice of the left piriform sinus by esophagography in this case, and made a diagnosis of acute suppurative thyroiditis caused by a piriform sin ...201020424880
crystallization and preliminary structural analyses of glutamate dehydrogenase from peptoniphilus asaccharolyticus.glutamate dehydrogenase (ec from peptoniphilus asaccharolyticus has been expressed as a selenomethionine-derivatized recombinant protein and diffraction-quality crystals have been grown that are suitable for structure determination. preliminary structural analyses indicate that the protein assembles as a homohexameric enzyme complex in solution, similar to other bacterial and mammalian enzymes to which its sequence identity varies between 25 and 40%. the structure will provide insight ...201020445250
innate inflammatory responses of human decidual cells to periodontopathic bacteria.the purpose of this study was to test the hypothesis that periodontopathic bacteria exert potent proinflammatory effects in human decidua.201020452492
antagonism and synergism in gardnerella vaginalis strains isolated from women with bacterial vaginosis.antagonistic and synergistic substances are important for interactions between micro-organisms associated with human body surfaces, either in healthy or in diseased conditions. in the present study, such compounds produced by gardnerella vaginalis strains isolated from women with bacterial vaginosis (bv) were detected in vitro and the antagonistic ones were partially characterized. among 11 g. vaginalis strains tested, all showed antagonistic activity against at least one of the 22 indicator bac ...201020466841
lemierre-like syndrome: a series of unique cases. 201020488807
competition between yogurt probiotics and periodontal pathogens in investigate the competition between probiotics in bio-yogurt and periodontal pathogens in vitro.201020491536
lead identification and optimization in crude samples using label free resonant acoustic profiling.detecting the molecular basis of protein-protein recognition is an essential element in understanding protein function because their ability to form specific complexes with other proteins underlies most cellular processes. the use of labels has limitations, such as changes to the binding kinetics due to the alterations in structure and function that occur with label addition, difficulty in detecting biochemical activities and the need for additional steps in assay development. these issues have ...201020549603
[identification of anaerobic bacteria isolated from various clinical specimens and determination of antibiotic susceptibilities].routine isolation, identification and susceptibility testing of anaerobic bacteria present several difficulties leading to defects in the determination of local susceptibility patterns which will guide empirical treatment protocols. this study was carried out to identify the anaerobic bacteria isolated from various clinical materials obtained from the suspected patients with anaerobic infection and to determine the antibiotic susceptibilities against several antibiotics. one hundred clinical spe ...201020549955
the pott's puffy tumor: an unusual complication of frontal sinusitis, methods for its detection.this is a case report of a pott's puffy tumor, characterized by a subperiosteal abscess associated with frontal bone osteomyelitis, as a consequence of a frontal sinusitis, in a 15-year-old boy. pott's puffy tumor is a rare condition usually seen as a complication of frontal sinusitis and more commonly described in children. given that, superficial temporal artery pseudoaneurysms might be interpreted as a cyst or lipoma, it is imperative that physicians be aware of their presentation.201020653869
microbiota around teeth and dental implants in periodontally healthy, partially edentulous patients: is pre-implant microbiological testing relevant?this study aimed to assess the prevalence of seven periodontal marker pathogens, before implant placement and 1 yr after loading, in periodontally healthy individuals and to assess the long-term effectiveness of pre-implant reduction of pathogens to below threshold levels. in 93 individuals needing single tooth replacement, pooled subgingival microbiological samples from standard sites were cultured and analyzed before implant treatment and 1 yr after loading. threshold levels commonly used in p ...201020662908
epidemiology of diabetic foot and management of foot problems in india.diabetes the global epidemic is rapidly increasing at an alarming rate. developing countries like india harbor the majority of diabetic people and by the year 2030 ad india will have the largest number of diabetic patients. diabetic foot is one of the common diabetic complications found in india. both aerobic and anaerobic pathogens form the etiology for diabetic foot infection. members of the enterobacteriaceae family were the most prominent among the aerobes while members of the genus peptostr ...201020705622
efficacy and safety of moxifloxacin in uncomplicated pelvic inflammatory disease: the monalisa evaluate the efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole in uncomplicated pelvic inflammatory disease (upid) in asia.201020716255
subgingival microbial profiles of smokers with periodontitis.the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ...201020739702
aerobic and anaerobic bacteriology of chronic adenoid disease in children.bacterial cultures from nasopharyngeal swabs of children after adenoidectomy and from the removed adenoid tissue in the same patient group were compared. at the same time, the colony-forming unit, as a measure of viable bacterial cells and the composition of isolated bacteria were also determined in the case of adenoid tissue. our findings showed that the culture results of nasopharyngeal swabs and inner part of the adenoid tissue are in close correlation. polymicrobial aerobic-anaerobic flora w ...201020810172
microbiology of acute complicated bacterial sinusitis at the university of the witwatersrand.a retrospective chart review study at two referral hospitals identified 226 consecutive surgical patients with acute complicated sinusitis.201020822623
peptostreptococcus russellii sp. nov., isolated from a swine-manure storage pit.a polyphasic taxonomic study was performed on seven strains of an unknown gram-positive, nonspore-forming, obligately anaerobic coccus-shaped bacterium isolated from a swine-manure storage pit. comparative 16s rrna gene sequencing confirmed that all these isolates were highly related to each other and formed a hitherto unknown linage within the clostridial rrna xi cluster of organisms. pairwise analysis demonstrated that the novel organism to be most closely related to peptostreptococcus anaerob ...201020833884
encapsulating peritoneal sclerosis: a single-center experience and review of the literature.encapsulating peritoneal sclerosis (eps) is a serious and often fatal complication of long-term pd with severe malnutrition and poor prognosis. it causes progressive obstruction and encapsulation of the bowel. this retrospective study reviews our experience and that reviewed in the literature concerning eps. it refers to a total of 1966 patients treated with chronic pd between 1974 and 2008. twenty one of them (1.1%) developed eps, with the incidence increasing with the duration of pd. mean age ...201020924672
[an unusual endometritis].we report a case of pyometra without any related risk factor. this leads to a severe septic shock. evolution was successful because of quick surgical management. we report literature management of such infection. although pyometra is an uncommon disease, it should be kept in mind as delayed management may lead to fatal outcome.201120926206
bactericidal activity of stabilized chlorine dioxide as an endodontic irrigant in a polymicrobial biofilm tooth model system.the purpose of this study was to determine bactericidal efficacy of 0.04% stabilized chlorine dioxide, 3% sodium hypochlorite, 2% chlorhexidine gluconate, and sterile distilled water in a polymicrobial biofilm model.201020951304
oxygen tension modulates the cytokine response of oral epithelium to periodontal bacteria.there is an inverse relationship between pocket depth and pocket oxygen tension with deep pockets being associated with anaerobic bacteria. however, little is known about how the host tissues respond to bacteria under differing oxygen tensions within the periodontal pocket.201020955352
purification of antibodies using affinity chromatography.affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilised to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. it involves the exploitation of specific interactions between a binding affinity pair, such as those between an antibody and its associated antigen, or between any ligand and its associated binding receptor/protein. with the discovery of protein a in 1970, and, subsequently pr ...201120978976
specified species in gingival crevicular fluid predict bacterial diversity.analysis of gingival crevicular fluid (gcf) samples may give information of unattached (planktonic) subgingival bacteria. our study represents the first one targeting the identity of bacteria in gcf.201021049043
early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man.aim: to compare the early bacterial colonization and soft tissue health of mucosa adjacent to zirconia (zro(2) ) and titanium (ti) abutment surfaces in vivo. materials and methods: twenty edentulous subjects received two endosseous mandibular implants. the implants were fitted with either a zro(2) or a ti abutment (non-submerged implant placement, within-subject comparison, left-right randomization). sulcular bacterial sampling and the assessment of probing pocket depth, recession and bleeding o ...201021054554
Displaying items 2101 - 2200 of 2255