PMID(sorted ascending)
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004].tendency of isolated bacteria from infections in abdominal surgery during the period from april 2005 to march 2006 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 384 strains including 18 strains of candida spp. were isolated from 161 (70.3%) of 229 patients with surgical infections. one hundred and ninty-five strains were isolated from primary infections, and 171 strains were isolated from postoperative infections. from primary infecti ...200717612256
middle meatus bacteriology of acute rhinosinusitis in patients after irradiation of nasopharynx.patients who undergo radiotherapy for nasopharyngeal carcinoma (npc) tend to suffer from rhinosinusitis because irradiation causes damage to sinonasal tissue; however, their bacteriology is lacking in the literature. the aim of this study was to determine the bacteriology and antibiotic resistance in acute rhinosinusitis (ars) of these patients.200717621810
necrotizing fasciitis: a complication of percutaneous coronary revascularization.percutaneous coronary revascularization is rarely complicated by infection. necrotizing fasciitis (nf) is a severe, deep-seated, potentially life-threatening infection of fascia and subcutaneous tissues. we report herein 2 cases of nf in patients undergoing percutaneous transluminal coronary revascularization for treatment of acute coronary syndrome. these are the first 2 reported cases of nf associated with percutaneous coronary revascularization.200717626992
the effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by m1 and m2 assess the influence of co-culture with mineral trioxide aggregate (mta) on phagocytosis and the production of reactive oxygen intermediates (roi) and nitrogen (no) species and the arginase activity by m1 and m2 peritoneal macrophages.200717627697
bacteriology of diabetic foot lesions.clinical grading and bacteriological study of 107 patients with diabetic foot lesions revealed polymicrobial aetiology in 69 (64.4%) and single aetiology in 21 (19.6%). among 107 patients 62 had ulcer. of these 31 had mixed aerobes. twenty six patients with cellulitis and 12 with gangrene had more than 5 types of aerobes and anaerobes such as e. coli, klebsiella spp., pseudomonas spp., proteus spp., enterobactor spp., enterococci spp., clostridium perfringens, bacteroides spp., prevotella spp. a ...200417642727
microbial flora in orodental infections.the present study was carried out to compare the normal aerobic and anaerobic bacterial oral flora with flora from deep seated dental caries, gingivitis and adult periodontitis. all the samples belonging to both the control and study groups yielded microbes. aerobe / anaerobe ratio was high in normal flora (1.48) as compared to dental caries (0.9), gingivitis (0.72) and periodontitis (0.56). ninety seven percent of orodental infections were polymicrobial and three or more microbes were found in ...200317642993
anaerobes in bacterial vaginosis.four hundred high vaginal swabs were taken from patients attending gynaecology and obstetrics department of govt. medical college, amritsar. the patients were divided into four groups i.e. women in pregnancy (group i), in labour/post partum (group ii), with abnormal vaginal discharge or bacterial vaginosis (group iii) and asymptomatic women as control (group iv). anaerobic culture of vaginal swabs revealed that out of 400 cases, 212(53%) were culture positive. maximum isolation of anaerobes was ...200317642997
role of anaerobes in acute pelvic inflammatory disease.pouch of douglas aspirates were collected from 50 women with history and examination suggestive of acute pelvic inflammatory disease (pid) and 20 healthy women admitted for tubal ligation served as control. a total of 57 microorganisms were isolated from 37 patients out of 50 in study group. of 37 positive cultures 21(56.7%) were monomicrobial and 16(43.2%) were polymicrobial. most common symptom in study group was lower abdominal pain (90%), vaginal discharge (70%) and irregular bleeding (40%) ...200317643017
actinomycotic bacteraemia after dental assess role of oral flora in causing bacteraemia following dental manipulations.200217657035
anaerobes in pleuropulmonary infections.a total of 76 anaerobes and 122 aerobes were isolated from 100 patients with pleuropulmonary infections, e.g. empyema (64), pleural effusion (19) and lung abscess (13). in 14% of the patients, only anaerobes were recovered, while a mixture of aerobes and anaerobes was encountered in 58%. from all cases of lung abscess, anaerobic bacteria were isolated, alone (04) or along with aerobic bacteria (13). from empyema and pleural effusion cases, 65.6% and 68.4% anaerobes were recovered respectively. a ...200217657055
development of 16s rrna-based probes for the identification of gram-positive anaerobic cocci isolated from human clinical specimens.fluorescent probes targeted at 16s rrna were designed for peptostreptococcus anaerobius and peptostreptococcus stomatis (pana134), parvimonas micra (pamic1435), finegoldia magna (fmag1250), peptoniphilus asaccharolyticus (pnasa1254), peptoniphilus ivorii (pnivo731), peptoniphilus harei (pnhar1466), anaerococcus vaginalis (avag1280) and anaerococcus lactolyticus (alac1438), based on the 16s rrna sequences of reference strains and 88 randomly chosen clinical isolates. these strains were also used ...200717714522
periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: a case-control study.recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. this case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (cad) based on clinical and periodontal microbiologic parameters.200717760542
comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of fusobacterium nucleatum and peptostreptococcus (micromonas) micros.this study compared growth and susceptibility to different concentrations of sodium hypochlorite (naocl) of mono- and dual-species biofilms of fusobacterium nucleatum or peptostreptococcus micros in vitro at 24 hours or 96 hours. a mann-whitney u test revealed that although at 24 hours dual-species biofilms had similar viable counts to those of monospecies biofilms (p > 0.001), they were more resistant to naocl (p < 0.001). at 96 hours, both microorganisms had higher viable counts and were more ...200717804319
probing the determinants of coenzyme specificity in peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis.glutamate dehydrogenase (ec from peptostreptococcus asaccharolyticus has a strong preference for nadh over nadph as a coenzyme, over 1000-fold in terms of kcat/km values. sequence alignments across the wider family of nad(p)-dependent dehydrogenases might suggest that this preference is mainly due to a negatively charged glutamate at position 243 (e243) in the adenine ribose-binding pocket. we have examined the possibility of altering coenzyme specificity of the peptostreptococcus enz ...200717850332
an infected porous polyethylene orbital implant.a 75-year-old man underwent enucleation with placement of a porous polyethylene orbital implant (medpor, porex technologies, fairburn, ga, u.s.a.). over the next 5 years, he was seen on numerous occasions with socket discharge that was unresponsive to a variety of eyedrops. exposure and re-exposure of the implant occurred, and the implant was removed. histopathologic assessment was consistent with an infectious process within the implant. postoperatively, the patient's symptoms and signs resolve ...200717881998
breast abscess bacteriologic features in the era of community-acquired methicillin-resistant staphylococcus aureus epidemics.increasing rates of community-acquired methicillin-resistant staphylococcus aureus (mrsa) infections have also affected the microbial profile of breast abscesses.200717891866
purification and partial characterization of a bacteriocin produced by eikenella corrodens.the purpose of this study was to purify and characterize a bacteriocin produced by eikenella corrodens a32e2.200817922820
in vitro and in vivo antibacterial activities of dc-159a, a new fluoroquinolone.dc-159a is a new 8-methoxy fluoroquinolone that possesses a broad spectrum of antibacterial activity, with extended activity against gram-positive pathogens, especially streptococci and staphylococci from patients with community-acquired infections. dc-159a showed activity against streptococcus spp. (mic(90), 0.12 microg/ml) and inhibited the growth of 90% of levofloxacin-intermediate and -resistant strains at 1 microg/ml. the mic 90s of dc-159a against staphylococcus spp. were 0.5 microg/ml or ...200817938194
an improved picture of methyl dynamics in proteins from slowly relaxing local structure analysis of 2h spin relaxation.protein dynamics is intimately related to biological function. core dynamics is usually studied with 2h spin relaxation of the 13cdh2 group, analyzed traditionally with the model-free (mf) approach. we showed recently that mf is oversimplified in several respects. this includes the assumption that the local motion of the dynamic probe and the global motion of the protein are decoupled, the local geometry is simple, and the local ordering is axially symmetric. because of these simplifications mf ...200717941658
degradation of serine-containing oligopeptides by peptostreptococcus micros atcc 33270.microorganisms of peptostreptococcus micros are asaccharolytic, anaerobic gram-positive cocci that are frequently isolated from human oral sites such as periodontal pockets. preliminary study showed that several amino acids, including serine, enhanced slightly the growth of p. micros. therefore, we investigated the degradation of serine and serine-containing oligopeptides.200717949340
evaluation of the microbiota of primary endodontic infections using checkerboard dna-dna hybridization.the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp.200717949342
bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography.the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography.200717949344
peptostreptococcus micros cell wall elicits a pro-inflammatory response in human macrophages.peptostreptococcus micros is a gram-positive anaerobic bacterium associated with periodontitis, a chronic inflammatory disease affecting tooth-supporting tissues. in the present study, we investigated the response of human macrophages to stimulation with a cell wall preparation from p. micros. in addition, the effect of the preparation on the phosphorylation of macrophage kinases was studied. the preparation, which was non-toxic for macrophages, significantly increased the secretion of the pro-i ...200717956940
[late complications of a pseudo aneurysm of the left ventricle: thrombus infection and purulent pericarditis].pseudoaneurysm is a rare complication of left ventricle myocardial infarction. rupture with tamponade and sudden death is the usual outcome. surgical intervention remains the treatment of choice. long term survival cases without surgery are rare. infection of the thrombus is also a possible event. we report the case of a patient with postinfarction left ventricular pseudoaneurysm complicated by infection of the thrombus and purulent pericarditis involving a peptostreptococcus. infection must be ...200717963717
anion modulation of the 1h/2h exchange rates in backbone amide protons monitored by nmr spectroscopy.the ability of three anionic cosolutes (sulfate, thiocyanate, and chloride) in modulating the (1)h/(2)h exchange rates for backbone amide protons has been investigated using nuclear magnetic resonance (nmr) for two different proteins: the igg-binding domain of protein l (protl) and the glucose-galactose-binding protein (ggbp). our results show that moderate anion concentrations (0.2 m-1 m) regulate the exchange rate following the hofmeister series: addition of thiocyanate increases the exchange ...200717965190
actinomycetemcomitin: a new bacteriocin produced by aggregatibacter (actinobacillus) actinomycetemcomitans.aggregatibacter (actinobacillus) actinomycetemcomitans p(7-20) strain isolated from a periodontally diseased patient has produced a bacteriocin (named as actinomycetemcomitin) that is active against peptostreptococcus anaerobius atcc 27337. actinomycetemcomitin was produced during exponential and stationary growth phases, and its amount decreased until it disappeared during the decline growth phase. it was purified by ammonium sulphate precipitation (30-60% saturation), and further by fplc (mono ...200817990016
extensive epidural abscess with surgical treatment and long term follow up.spinal epidural abscess is an uncommon infection. there are few reports on extensive epidural abscesses.200717998130
severe versus local odontogenic bacterial infections: comparison of microbial was the aim of this study to evaluate the clinical and microbiological differences between severe and local odontogenic abscesses.200817998782
[the development and in vitro experiment study of a bio-type root canal filling sealer using calcium phosphate cement].the purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (cpc), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal.200718004487
the bacteriology and antimicrobial susceptibility of infected and non-infected dog bite wounds: fifty bite wounds are a common reason for dogs requiring veterinary care, but there is surprisingly little data on the bacteriology of bite wounds. a prospective study was performed on dogs with various grades of bite wound to identify the bacteria present in these wounds. swabs were collected from all wounds for bacterial culture and cytology. all swabs were cultured aerobically and anaerobically and all aerobic cultures were evaluated for antibiotic susceptibility using the kirby bauer disk diff ...200818029118
cephalosporins in overcoming beta-lactamase-producing bacteria and preservation of the interfering bacteria in the treatment of otitis, sinusitis and tonsillitis.the treatment of upper respiratory tract infections (urtis) is complicated by the resurgence of beta-lactamase-producing bacteria (blpb) and the absence of interfering bacteria. blpb can have a direct pathogenic impact in causing the infection as well as an indirect impact through their ability to produce the enzyme beta-lactamase. blpb may not only survive penicillin therapy but can also protect other penicillin-susceptible bacteria from penicillin. in this review, the clinical in vitro and in ...200718039079
[co-occurence of indol-producing bacterial strains in the vagina of women infected with chlamydia trachomatis].the aim of this study was to determine if cervicitis, caused by chlamydia trachomatis (c. trachomatis), has an influence on the frequency of occurrence of selected aerobic and anaerobic bacterial strains, connected with etiology of aerobic vaginitis (av) and bacterial vaginosis (bv). indole-producing bacteria have received particular attention due to their possibly inductive role in chronic cervicitis caused by c. trachomatis.200718050609
characterization of the protein unfolding processes induced by urea and temperature.correct folding is critical for the biological activities of proteins. as a contribution to a better understanding of the protein (un)folding problem, we studied the effect of temperature and of urea on peptostreptococcal protein l destructuration. we performed standard molecular dynamics simulations at 300 k, 350 k, 400 k, and 480 k, both in 10 m urea and in water. protein l followed at least two alternative unfolding pathways. urea caused the loss of secondary structure acting preferentially o ...200818065481
rapid identification of non-sporing anaerobes using nuclear magnetic resonance spectroscopy and an identification strategy.the non-sporing anaerobes cause a wide spectrum of infections. they are difficult to culture and their identification is tedious and time-consuming. rapid identification of anaerobes is highly desirable. towards this end, the potential of nuclear magnetic resonance (nmr) spectroscopy for providing a fingerprint within the proton spectrum of six genera belonging to anaerobes reflecting their characteristic metabolites has been investigated.200718087080
[a case of bacteremia by atopobium rimae in a patient with liver cirrhosis].atopobium rimae, previously lactobacillus rimae, is a strictly anaerobic, non-spore forming grampositive rod which was frequently isolated from odontogenic infection. we report a case of a. rimae bacteremia. a 47-yr-old man with liver cirrhosis was admitted to the hospital via emergency room due to fever and chill. his abdominal and pelvic computed tomography revealed a small abscess near the left adrenal gland. three sets of blood cultures were taken and non-spore forming, grampositive rods wer ...200718094600
generation and expression of a minimal hybrid ig-receptor formed between single domains from proteins l and g.the ig-binding properties of protein l from peptostreptococcus magnus and protein g from streptococcus have been successfully combined through the construction of a novel hybrid protein, consisting of a single ig-binding domain from each protein. the biophysical and biochemical properties of this construct have been characterized through equilibrium and pre-equilibrium fluorescence spectroscopy, circular dichroism, isothermal titration calorimetry, affinity chromatography, and conformational sta ...200818158250
european surveillance study on antimicrobial susceptibility of gram-positive anaerobic cocci.gram-positive anaerobic cocci (gpac) are a heterogeneous group of microorganisms frequently isolated from local and systemic infections. in this study, the antimicrobial susceptibilities of clinical strains isolated in 10 european countries were investigated. after identification of 299 gpac to species level, the minimum inhibitory concentrations of penicillin, imipenem, clindamycin, metronidazole, vancomycin and linezolid were determined by the agar dilution method according to the clinical and ...200818180149
detection and identification of bacterial dna in detect and identify bacteria in semen by sequencing polymerase chain reaction (pcr)-amplified ribosomal rna gene regions (rdnas).200818191853
descending necrotizing mediastinitis secondary to retropharyngeal abscess without cervical spread.cervical infections can cause neck phlegmonosa and occasionally develop lethal mediastinitis. we report a 52-year-old man with a retropharyngeal abscess causing descending mediastinis without cervical spread. thoracoscopic drainage without cervicotomy was successful. deep neck infections causing a descending mediastinitis should be considered in patients even without an abnormal neck appearance.200818213468
anaerobic bacterial pericardial effusion in a cat.a 9-year-old male cat was presented for evaluation of chronic weight loss and was subsequently diagnosed with pericardial effusion. the effusion was quantified as a septic exudate caused by the anaerobic bacterium peptostreptococcus. antibiotic therapy resulted in complete resolution of the pericardial effusion. as peptostreptococcus is a common oral bacterium and the cat had a previous dental procedure, it is speculated that the pericardial effusion was secondary to bacteraemia from the dental ...200718237044
characterization of chorioamnionitis in 2nd-trimester c-section placentas and correlation with microorganism recovery from subamniotic tissues.prolonged exposure to infection appears to influence fetal/neonatal development. we characterize the relationship between histologic patterns of inflammation and microorganism recovery from the placentas of live born infants delivered before the 28th postmenstrual week. the subamniotic parenchyma of 835 placentas delivered by cesarean section were cultured and evaluated for specific histologic patterns of inflammation in a blinded fashion. cases with prolonged membrane rupture were excluded. mic ...200818237241
complete genome sequence of finegoldia magna, an anaerobic opportunistic pathogen.finegoldia magna (formerly peptostreptococcus magnus), a member of the gram-positive anaerobic cocci (gpac), is a commensal bacterium colonizing human skin and mucous membranes. moreover, it is also recognized as an opportunistic pathogen responsible for various infectious diseases. here, we report the complete genome sequence of f. magna atcc 29328. the genome consists of a 1,797,577 bp circular chromosome and an 189,163 bp plasmid (ppep1). the metabolic maps constructed based on the genome inf ...200818263572
smap29 congeners demonstrate activity against oral bacteria and reduced toxicity against oral keratinocytes.cathelicidins are antimicrobial peptides found in epithelial and mucosal tissues as well as the secondary granules of neutrophils. smap29, a sheep cathelicidin, has differential antimicrobial properties against various pathogens, including periodontal organisms. the purpose of this study was to evaluate the antimicrobial properties and cytotoxicity of smap29, smap28, and three congeners (smap18a, smap18d, and smap14a).200818279175
microbiology and management of abdominal infections.this review describes the microbiology, diagnosis, and management of intra-abdominal infections. these infections include secondary peritonitis, abdominal abscesses, and cholangitis. the infection generally occurs because enteric microorganisms enter the peritoneal cavity through a defect in the wall of the intestine or other viscus as a result of obstruction, infarction, or direct trauma. mixed aerobic and anaerobic flora can be recovered. the predominant aerobic isolates are escherichia coli, ...200818288616
isolation of obligate anaerobic bacteria from ulcerative keratitis in domestic determine the frequency of obligate anaerobic bacterial isolation from corneal samples of domestic animals with ulcerative keratitis and to characterize the historical, clinical, cytological, and microbiological features of culture-positive cases.200818302576
[relationship between clostridium difficile associated diarrhea and intestinal microecosystem disorder in patients received allogeneic hematopoietic stem cell transplantation].this study was to investigate the relationship between clostridium difficile associated diarrhea (cdad) and intestinal microecosystem in patients received allogeneic hematopoietic stem cell transplantation (allo-hsct) and to clarify clinical characteristics of intestinal microecosystem disorder. clostridium difficile (cd) was isolated and identified by enzyme-linked-immunosorbent assay using clostridium difficile premier toxins a&b kit and anaerobic culture in 44 cases with diarrhea. fecal flora ...200818315917
anaerobic liver abscesses as initial presentation of silent colonic cancer.rarely, multiple liver abscesses caused by anaerobic organisms are the presenting form of unsuspected colonic cancer in the absence of liver metastases.200418333045
urea and guanidinium chloride denature protein l in different ways in molecular dynamics performing protein-denaturation experiments, it is common to employ different kinds of denaturants interchangeably. we make use of molecular dynamics simulations of protein l in water, in urea, and in guanidinium chloride (gdmcl) to ascertain if there are any structural differences in the associated unfolding processes. the simulation of proteins in solutions of gdmcl is complicated by the large number of charges involved, making it difficult to set up a realistic force field. furthermore, at ...200818339753
moraxella catarrhalis-dependent tonsillar b cell activation does not lead to apoptosis but to vigorous proliferation resulting in nonspecific igm production.the respiratory pathogen moraxella catarrhalis has a high affinity for human igd and is mitogenic for peripheral blood b lymphocytes. moraxella igd-binding protein, which is a multifunctional outer membrane protein with adhesive properties, is responsible for the interaction. previous experiments with the ig-binding b cell superantigens protein a and protein l from staphylococcus aureus and peptostreptococcus magnus, respectively, have suggested that nonimmune bcr cross-linking induces b cell ap ...200818372337
[lumbar pyomyositis caused by finegoldia magna (peptostreptococcus magnus) in a patient with hepatitis c virus infection]. 200818373916
a retrospective analysis of isolates from patients with vaginitis in a private greek obstetric/gynecological hospital (2003-2006).vaginitis is a common cause of complaints in women in various parts of the world. through our literature search, we identified noteworthy differences in the etiology of vaginitis based on the geographic location. this study was conducted to describe microbial etiology in a population of symptomatic women with vaginitis in greece.200818376352
clinical and microbiological determinants of ailing dental implants.the failure of the host tissue to establish or maintain osseointegration around dental implants is due to either occlusal or parafunctional forces, premature loading, ill-directed stress, or microbial infection. the long-term failure rate of dental implants is generally 5-10%. although a variety of etiologies of early peri-implant bone loss (from implant placement to 1-year post-loading) have been proposed, factors associated with late implant failures are less well understood but are probably r ...200918384407
microbiology and management of joint and bone infections due to anaerobic describes the microbiology, diagnosis, and management of septic arthritis and osteomyelitis due to anaerobic bacteria.200818392922
ocular infections due to anaerobic bacteria in children.the recent increased recovery of anaerobic bacteria from children has led to greater appreciation of the role these organisms play in pediatric infections at all body sites, including the eye. in studies that employed adequate methods for recovery of anaerobes, they were isolated from approximately one-third of the children with conjunctivitis, half of the time in pure culture. the predominant recovered anaerobes were clostridium spp., gram-negative anaerobic bacilli, and peptostreptococcus spp. ...200818404954
[microbiological and biochemical characteristics of inflammatory tissues in the periodontium].to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva.200818413987
infection associated with hematoma formation after shoulder arthroplasty.hematoma formation requiring operative treatment after shoulder arthroplasty may be associated with higher patient morbidity. we therefore determined whether there was an association of hematoma formation requiring operative treatment with deep infection after shoulder arthroplasty. between 1978 and 2006, we performed 4147 shoulder arthroplasties in 3643 patients. of these, 12 shoulders (0.3%) underwent reoperation for hematoma formation. the mean time interval from arthroplasty to surgery for t ...200818421541
microbial analysis of canals of root-filled teeth with periapical lesions using polymerase chain reaction.the objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. dna was extracted from 45 canal samples of root-filled teeth with periapical lesions. a pcr assay using species-specific primers of 16s rdna and the downstream intergenic spacer region was used for microbial detection. enterococcus faecalis ...200818436030
photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial.recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ...200918465191
nonpuerperal breast infection.we undertook a microbiological study of purulent specimens from women with symptomatic breast abscesses.199518476022
assessment of the microbiota in root canals of human primary teeth by checkerboard dna-dna hybridization.the purpose of this study was to evaluate in vivo the prevalence of microorganisms in root canals of human primary teeth, by checkerboard dna-dna hybridization.200718477431
campylobacter species isolated from extra-oro-intestinal abscesses: a report of four cases and literature review.campylobacter species are frequently isolated from fecal specimens of patients with diarrheal illness. several campylobacter species are commonly isolated from the oral cavity. in contrast, campylobacter species are rarely isolated from extra-oro-intestinal abscesses. reported here are four cases of extra-oro-intestinal abscesses due to polymicrobial flora, including campylobacter species. the first case is a 35-year-old woman who was diagnosed with a brain abscess caused by c. gracilis, strepto ...200818488257
changes in abundance of lactobacillus spp. and streptococcus suis in the stomach, jejunum and ileum of piglets after weaning.this present study investigated the changes in bacterial community composition, with an emphasis on lactobacillus spp. and streptococcus suis populations as potentially beneficial and harmful groups, in the stomach, jejunum and ileum of piglets after weaning (21 days postpartum) by 16s rrna gene-based methods. denaturing gradient gel electrophoresis analysis showed that, after weaning, predominant bands related to lactobacillus spp. disappeared and were replaced by potential pathogenic species, ...200818554303
influence of local tetracycline on the microbiota of alveolar osteitis in rats.the aim of the present study was to evaluate the effects of local tetracycline on the occurrence of alveolar osteitis in rats, and on the microbiota associated to this infection. forty wistar rats were randomly assigned to 4 groups (n=10): i - the rats had the maxillary right incisor extracted and the alveolar wound did not receive any treatment; ii - adrenaline and ringer-pras were introduced into the alveolar wound; iii - the alveolar wound was irrigated with sterile saline; and iv - the alveo ...200818568225
quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems.transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ...200818579722
deep brain stimulation for holmes' tremor related to a thalamic abscess.medical treatment is usually ineffective for holmes' tremor, and surgery is the treatment of choice for many patients. here we report the case of a 14-year-old girl who developed holmes' tremor related to a thalamic abscess and was successfully treated with thalamic deep brain stimulation.200818581123
study of gaseous substrate fermentations: carbon monoxide conversion to acetate. 1. batch culture.biological processes may be used to convert gas phase substrates, such as h(2)s, ch(4), co, h(2), and co(2), to useful products. utilization of these substrates is often a mass transfer limited process, first requiring absorption across the gas-liquid interface and diffusion through the culture medium to the cell surface, prior to reaction. this article presents a method for determining fermentation parameters of a gaseous substrate in convenient batch vessels using a modified monod model. the p ...198918588164
study of gaseous substrate fermentations: carbon monoxide conversion to acetate. 2. continuous culture.the fermentation of gaseous substrates such as co, h(2), and co(2) may be performed in a continuous stirred tank reactor, as well as the traditional batch reactor. in this article, the conversion of carbon monoxide by peptostreptococcus productus is demonstrated in a stirred tank reactor under both mass transfer-controlled and nonmass transfer-controlled conditions. utilizing a non-steady-state procedure, intrinsic rates are evaluated under non-mass transfer-controlled conditions in a time perio ...198918588165
relationship of specific vaginal bacteria and bacterial vaginosis treatment failure in women who have sex with women.bacterial vaginosis frequently persists after treatment. the role of newly defined bacterial vaginosis-associated bacteria (bvab), which have a specificity for this condition of 97% or greater, has not been assessed.200818591634
a case of necrotizing fasciitis due to streptococcus agalactiae, arcanobacterium haemolyticum, and finegoldia magna in a dog-bitten patient with diabetes.we report a case of necrotizing fasciitis involving streptococcus agalactiae, arcanobacterium haemolyticum, and finegoldia magna in a 36-yr-old female diabetic patient, which started after a minor dog bite to the toe of the patient. this case suggested that a trivial infection after a minor dog bite in an immunocompromised patient such as diabetes patient could result in a significant complication such as necrotizing fasciitis. the life-threatening infection was cured by timely above-the-knee am ...200818594170
increasing trends in antimicrobial resistance among clinically important anaerobes and bacteroides fragilis isolates causing nosocomial infections: emerging resistance to carbapenems.this study reports data on the susceptibilities to five commonly used antianaerobic agents of five clinically frequently encountered anaerobes from 2000 to 2007 and to bacteroides fragilis isolates causing nosocomial infections from 1990 to 2006. there was a trend of decreasing susceptibilities of these anaerobes to ampicillin-sulbactam, cefmetazole, chloramphenicol, and clindamycin with time during the study period. the rates of susceptibility to clindamycin and cefmetazole for all clinical iso ...200818625771
formulation and evaluation of pla and plga in situ implants containing secnidazole and/or doxycycline for treatment of periodontitis.the purpose of this study is to formulate in situ implants containing doxycycline hydrochloride and/or secnidazole that could be used in the treatment of periodontitis by direct periodontal intrapocket administration. biodegradable polymers [poly (lactide) (pla) and poly (lactide-co-glycolide) (plga)], each polymer in two concentrations 25%w/w, 35%w/w were used to formulate the in situ implants. the rheological behavior, in vitro drug release and the antimicrobial activity of the prepared implan ...200818654864
bacterial colonization of oral implants from nondental sources.implants showing signs of peri-implantitis harbor a microbiota similar to that of periodontitis-affected teeth. this case report describes the subgingival microbiota of a 45-year-old female with advanced periodontitis before and after complete edentulation and reconstruction with dental implants. a 3-month healing period post extraction passed before implants were placed using a two-stage submerged implant protocol. at 4- to 6-month recall visits after definitive prosthetic reconstruction, some ...200918657153
alternate arrangement of ppl b3 domain and spa d domain creates synergistic double-site binding to vh3 and vkappa regions of our previous study, a kind of novel hybrid immunoglobulin (ig)-binding proteins (ibps) was obtained with the characteristic structure of alternately arranged finegoldia magna (formerly peptostreptococcus magnus) protein l (p. magnus protein l, ppl) b3 domain (b3) and staphylococcal protein a (spa) d domain (d). in this study, two representative molecules of these novel proteins, ld3 (b3-d-b3) and ld5 (b3-d-b3-d-b3) (ld3/5), showed substantially higher affinity for igg-f(ab')2, igm, and iga th ...200818694300
value of anaerobic culture in bacterial surveillance program for platelet concentrates.short-term aerobic bacterial culture (stabc) has been used routinely in hong kong since 1998 to reduce bacterial contamination in platelet concentrates (pcs) with good results. with more countries implementing routine aerobic and anaerobic cultures of pcs, a prospective study was conducted to determine the value of anaerobic culture to stabc.200818694462
evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (ig)-binding proteins (ibps) with four kinds of ig molecules.protein a, protein g and protein l are three well-defined immunoglobulin (ig)-binding proteins (ibps), which show affinity for specific sites on ig of mammalian hosts. although the precise functions of these molecules are not fully understood, it is thought that they play an important role in pathogenicity of bacteria. the single domains of protein a, protein g and protein l were all demonstrated to have function to bind to ig. whether combinations of ig-binding domains of various ibps could exh ...200818700046
clinicopathologic challenge. gangrenous cellulitis caused by peptostreptococcus. 200818717855
the influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice.this study assessed the influence of mineral trioxide aggregate (mta) on adaptive immune responses. balb/c mice were immunized with heat-killed fusobacterium nucleatum (fn) in mta or other control adjuvants, and serum igg responses to fn were measured. either fn- or peptostreptococcus anaerobius (pa)-reactive memory t cells (tm) were preincubated in vitro with/without mta and restimulated with fn or pa. tm proliferation and cytokine production were assessed. compared with control groups, immunog ...200818718367
detection of anaerobic prosthetic joint infection by pcr and dna sequencing--a case report. 200818766493
bisphosphonate-associated osteonecrosis: a clinician's reference to patient management.bisphosphonates (bps), as inhibitors of osteoclasts, are widely used in the management of metastatic bone disease and in the prevention of osteomalacia and osteoporosis. recent cases of bone necrosis of the jaws have been associated with the use of bisphosphonate therapy. a case is presented of a patient with osteonecrosis of the maxilla with a history of long-term bisphosphonate therapy for metastatic breast cancer. the authors treated the patient and suggest appropriate patient management guid ...200818767452
root canal microbiota of teeth with chronic apical periodontitis.samples from infected root canals of 43 teeth with chronic apical periodontitis were analyzed for the presence and relative levels of 83 oral bacterial species and/or phylotypes using a reverse-capture checkerboard hybridization assay. associations between the most frequently detected taxa were also recorded. the most prevalent taxa were olsenella uli (74%), eikenella corrodens (63%), porphyromonas endodontalis (56%), peptostreptococcus anaerobius (54%), and bacteroidetes oral clone x083 (51%). ...200818768651
[peptostreptococcus endocarditis: presentation of two cases and literature review].we describe two new cases of peptostreptococcus endocarditis, one case of peptostreptococcus micros prosthetic valve endocarditis and the other of peptostreptococcus assaccharolyticus tricuspid native valve endocarditis in an intravenous drug user (idu) patient and review nine cases previously reported.200818769744
photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial.recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment.200818771363
in vitro bactericidal activity of recombinant human beta-defensin-3 against pathogenic bacterial strains in human tooth root canal.human beta-defensin-3 (hbd3), an endogenous antimicrobial peptide, has strong broad-spectrum antimicrobial activity. this study aimed to obtain recombinant hbd3 (rhbd3) and to test the hypothesis that the antimicrobial characteristics of hbd3 may offer an advantage over conventional medicine in reducing intracanal bacteria. genetic engineering was used to obtain active rhbd3 and analysis revealed that it exhibited a broad spectrum of antibacterial activity at low micromolar concentrations agains ...200918775647
targeted antimicrobial activity of a specific igg-smap28 conjugate against porphyromonas gingivalis in a mixed culture.antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. to assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (smap) 28 was linked to affinity- and protein g-purified rabbit immunoglobulin g (igg) antibodies specific to the outer sur ...200918778918
pericarditis caused by anaerobic bacteria.this review describes the microbiology, diagnosis and management of pericarditis due to anaerobic bacteria. the predominant anaerobes isolated from patients with pericarditis are gram-negative bacilli (mostly bacteroides fragilis group) as well as peptostreptococcus, clostridium, fusobacterium, bifidobacterium and actinomyces spp. anaerobic bacteria can be recovered from pericarditis resulting from the following mechanisms: (i) spread from a contiguous site of infection, either de novo or follow ...200918789852
the role of toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens.periapical lesions arise as a result of the activation and interaction of the host immune responses against root canal infection. recently identified toll-like receptors (tlr) seem to be involved in the recognition and development of immune responses against a myriad of microorganisms. however, very little information is available on the role of tlr in the induction of periapical lesions.200818793356
rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by facilitate the identification of anaerobes cultivated from periodontal disease, whole cell bacterial identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) was evaluated.200818793359
molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain was the aim of the present study to evaluate root canal samples for the presence and numbers of specific species as well as for total bacterial load in teeth with chronic apical periodontitis using quantitative real-time polymerase chain reaction (pcr).200818793361
orbital abscess secondary to acute dacryocystitis: case report.acute dacryocystitis usually induces preseptal infection. in rare instances the infection that is confined to the lacrimal sac can extend to the orbital contents resulting in orbital cellulitis. we present a case of intraconal abscess secondary to acute dacryocystitis and review the literature of orbital cellulitis resulting from acute lacrimal sac infection.200818797671
bacteriological and clinical evaluation of twelve cases of post-surgical sepsis of odontogenic tumours at a referral determine the bacterial aetiology of sepsis occurring following surgery of odontogenic tumours and assess the effect of prompt and proper antibiotic usage.200818817023
rationally designed ligands for use in affinity chromatography: an artificial protein l.synthetic affinity ligands can circumvent the drawbacks of natural immunoglobulin (ig)-binding proteins by imparting resistance to chemical and biochemical degradation and to in situ sterilization, as well as ease and low cost of production. protein l (ppl), isolated from peptostreptococcus magnus strains, interacts with the fab (antigen-binding fragment) portion of igs, specifically with kappa light chains, and represents an almost universal ligand for the purification of antibodies. the concep ...200818826050
polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded flx amplicon pyrosequencing (btefap).diabetic extremity ulcers are associated with chronic infections. such ulcer infections are too often followed by amputation because there is little or no understanding of the ecology of such infections or how to control or eliminate this type of chronic infection. a primary impediment to the healing of chronic wounds is biofilm phenotype infections. diabetic foot ulcers are the most common, disabling, and costly complications of diabetes. here we seek to derive a better understanding of the pol ...200818833331
correlates of periodontal decline and biologic markers in older adults.there is limited information on infectious and host responses distinguishing older people with or without active periodontitis. this study measured bacterial and serum cytokine and high-sensitivity c-reactive protein (hscrp) levels in older persons.200818834247
purulent pericarditis with cardiac tamponade caused by anaerobic agents.purulent pericarditis is a rare disease associated with high mortality, even with appropriate antibiotic therapy. the authors report the case of a patient with purulent pericarditis with no identifiable primary source that presented clinically as cardiac tamponade. the patient required emergency pericardiocentesis and surgical pericardiectomy due to persisting signs of tamponade. microbiology cultures isolated anaerobic agents of fusobacterium spp., corynebacterium spp. and peptostreptococcus. t ...200818959092
uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth.intra-amniotic infection and inflammation are major causes of preterm birth (ptb). however, intra-amniotic inflammation is often detected in the absence of infection. this may partly be due to the culturing methods employed in hospital laboratories, which are unable to detect the uncultivated species. in this study, intra-amniotic microbial infections associated with ptb were examined by both culture and 16s rrna-based culture-independent methods and were corroborated by the presence of intra-am ...200918971361
difficult intubation provokes evaluate the prevalence of bacteremia after mask ventilation, laryngoscopy, and endotracheal intubation before induction of general anesthesia and to discover any correlation between traumatic manipulations and bacteremia. the specific bacteria responsible, knowledge of which may guide the prophylactic use of antibiotics, also were investigated.200818983229
spondylodiscitis due to peptostreptococcus spp: a case report. 200919022696
frequency, microbial interactions, and antimicrobial susceptibility of fusobacterium nucleatum and fusobacterium necrophorum isolated from primary endodontic infections.this study assessed the prevalence and microbial interactions of fusobacterium nucleatum and fusobacterium necrophorum in primary endodontic infections from a brazilian population and their antimicrobial susceptibility to some antibiotics by the e-test. one hundred ten samples from infected teeth with periapical pathologies were analyzed by culture methods. five hundred eighty individual strains were isolated; 81.4% were strict anaerobes. f. nucleatum was found in 38 root canals and was associat ...200819026872
the effect of a supragingival plaque-control regimen on the subgingival microbiota in smokers and never-smokers: evaluation by real-time polymerase chain reaction.the aim of the present study was to evaluate the effect of strict supragingival plaque control on the subgingival microbiota in smokers and never-smokers. research into the impact of supragingival plaque control on the number of subgingival bacteria has resulted in contradictory findings. real-time polymerase chain reaction (pcr) has been suggested as a valid alternative to current microbiologic methods based on bacteria cultures.200819053920
infective endocarditis caused by anaerobic bacteria.this review summarizes the microbiology, diagnosis and management of infective endocarditis (ie) due to anaerobic bacteria. anaerobic bacteria are an uncommon but important cause of ie. most cases are caused by the anaerobic and microaerophilic streptococci propionibacterium acnes, bacteroides fragilis and clostridium species. predisposing factors and signs and symptoms of ie caused by anaerobic bacteria are similar to those seen in ie due to facultative anaerobic bacteria with the following exc ...200819056073
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