TitleAbstractYear(sorted descending)
notes on the microbiology of cholesteatoma: clinical findings and treatment.anomalous proliferation of the cholesteatoma epithelium is caused by extrinsic factors such as toxins or bacterial antigens combined with lytic enzymes, lymphokines and cytokines released from the inflammatory infiltrate. this could explain the close relationship between the aggressiveness of cholesteatoma and repeated bacterial superinfection, therefore it is very important to know the bacteria involved in order to control the regrowth of skin following surgery, reduce the aggressive potential ...200920161877
assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis.the aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (t-rflp), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features.200919702949
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
meningitis and epidural abscess related to pansinusitis.sinusitis can rarely be latent and present directly with intracranial complications. we present the case of an 11-year-old girl who presented with typical features of meningitis. she underwent neuroimaging because of slow improvement and concern for a brain abscess. despite no history or examination findings suggestive of sinusitis, she was found to have pansinusitis with intracranial extension causing meningitis and epidural abscess.200919369843
the bacteriology of salivary gland infections.the parotid gland is the salivary gland most commonly affected by inflammation. however, infection of the salivary glands can occur in any of the glands. the most common pathogens associated with acute bacterial infection are staphylococcus aureus and anaerobic bacteria. the predominant anaerobes include: anaerobic gram negative bacilli (eg, pigmented prevotella and porphyromonas); fusobacterium spp; and peptostreptococcus spp. in addition, streptococcus spp (including streptococcus pneumoniae) ...200919608044
effect of age on gingival crevicular fluid concentrations of mif and pge2.we used an experimental gingivitis study design to compare crevicular fluid concentrations of migration inhibitory factor (mif) and prostaglandin e(2) (pge(2)) in younger (18 to 30 yrs) and older (46 to 77 yrs) healthy adults. pge(2) increased after 1 wk in younger participants, whereas it decreased in older individuals after 1 wk of plaque accumulation. a significant interaction between age and time was observed for pge(2) (p = 0.04). high concentrations of mif were identified in both age group ...200919641151
bone microbial contamination influences autogenous grafting in sinus augmentation.the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation.200919656037
presence of aggregatibacter actinomycetemcomitans in young individuals: a 16-year clinical and microbiological follow-up look for clinical signs of periodontal disease in young adults who exhibited radiographic bone loss and detectable numbers of aggregatibacter actinomycetemcomitans in their primary dentition.200919678862
significance of cytomorphological and microbiological examination of bile collected by endoscopic cannulation of the papilla of vater.bile analysis yields important information such as "biliary microlithiasis" and biliary tract colonization by microorganisms like salmonella typhi or paratyphi, escherichia coli, etc., which may progresses to cholelithiasis and have been found to have a role in the development of gallbladder cancer in india.200919679953
identification of a mechanical rheostat in the hydrophobic core of protein l.the ability of proteins and their complexes to withstand or respond to mechanical stimuli is vital for cells to maintain their structural organisation, to relay external signals and to facilitate unfolding and remodelling. force spectroscopy using the atomic force microscope allows the behaviour of single protein molecules under an applied extension to be investigated and their mechanical strength to be quantified. protein l, a simple model protein, displays moderate mechanical strength and is t ...200919683005
the antimicrobial effects of hops (humulus lupulus l.) on ruminal hyper ammonia-producing assess the antimicrobial effects of hops (humulus lupulus l.) on hyper ammonia producing-bacteria (hab), which catabolize amino acids and peptides in the bovine rumen.200919413813
mechanical characterization of protein l in the low-force regime by electromagnetic tweezers/evanescent nanometry.mechanical manipulation at the single molecule level of proteins exhibiting mechanical stability poses a technical challenge that has been almost exclusively approached by atomic force microscopy (afm) techniques. however, due to mechanical drift limitations, afm techniques are restricted to experimental recordings that last less than a minute in the high-force regime. here we demonstrate a novel combination of electromagnetic tweezers and evanescent nanometry that readily captures the forced un ...200919413987
the microbiota on different oral surfaces in healthy children.knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes.200919416446
ten-year follow-up of a patient with osler-weber-rendu syndrome and recurrent cerebral abscess secondary to pulmonary arteriovenous 1998, one of us (mjm) published an article discussing several patients with osler-weber-rendu syndrome or hereditary haemorrhagic telangiectasia with secondary cerebral complications. these were intracerebral haemorrhage, ischaemic infarct and intracerebral abscess. we outlined the background of the syndrome as well as its genotype and predicted an increasingly important role for genetic testing. our aim in this paper is to examine the progress of one of the patients as well as recommending f ...200919428258
the effects of treatment of acute otitis media with a low dose vs a high dose of amoxicillin on the nasopharyngeal compare the effects on the nasopharyngeal flora of therapy of acute otitis media in children with either a low dose or a high dose of amoxicillin.200919451466
fetal exposure to oral pathogens and subsequent risk for neonatal intensive care admission.maternal periodontal infection has been associated with adverse maternal and neonatal outcomes. in utero fetal exposure to oral pathogens was also recognized as deleterious to the fetus. the objective of this study was to determine the relationship between fetal exposure to oral pathogens and neonatal intensive care unit (nicu) admission.200919485816
[oral cavity microbiocenose in healthy teenagers and patients with chronic gastritis and gastroduodenitis].microbial association distinctive features and colonization degree of different oral cavity biotope were established in healthy teenagers and patients with chronic gastritis and gastroduodenitis. there were studied mucous membrane surfaces of cheek, tongue, content of gingival crevice, oral fluid and dental plaque of 24 healthy persons of the age 12-17 years and 29 patients of the same age with gastritis and gastroduodenitis. on the mucous membranes of the ill teenagers there were found porphiro ...200919491778
starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus.the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ...200919540450
descending necrotizing soft tissue infection of periodontal origin.descending necrotizing soft tissue infections involving the neck and chest are a rare but hazardous sequela of periodontal infections. these infections are associated with significant mortality.the high mortality rates of this condition are attributed to significant delays in diagnosis and treatment of common periodontal infections that have progressed to severe disease without overt clinical signs or symptoms. as with all necrotizing soft tissue infections, making a timely diagnosis requires th ...200919546678
comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples.200919563330
molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile.the microbial etiology of periradicular lesions is recognized; however, the bacterial profile of these lesions is not well elucidated. the purpose of this study was to determine the frequency of bacterial colonization in these lesions and to characterize the bacterial community present in root ends and periradicular lesions.200919567313
two teenagers with sepsis and pneumonia. 200919114923
effectiveness of ozone against endodontopathogenic microorganisms in a root canal biofilm assess the antimicrobial efficacy of aqueous (1.25-20 microg ml(-1)) and gaseous ozone (1-53 g m(-3)) as an alternative antiseptic against endodontic pathogens in suspension and a biofilm model.200919125975
the microbiota of acute apical the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ...200919131319
uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth.intra-amniotic infection and inflammation are major causes of preterm birth (ptb). however, intra-amniotic inflammation is often detected in the absence of infection. this may partly be due to the culturing methods employed in hospital laboratories, which are unable to detect the uncultivated species. in this study, intra-amniotic microbial infections associated with ptb were examined by both culture and 16s rrna-based culture-independent methods and were corroborated by the presence of intra-am ...200918971361
the ability of non-bacteriocin producing streptococcus bovis strains to bind and transfer bovicin hc5 to other sensitive bacteria.streptococcus bovis hc5 produces a broad spectrum lantibiotic (bovicin hc5), but s. bovis jb1 does not have antimicrobial activity. preliminary experiments revealed an anomaly. when s. bovis jb1 cells were washed in stationary phase s. bovis hc5 cell-free culture supernatant, the s. bovis jb1 cells were subsequently able to inhibit hyper-ammonia producing ruminal bacteria (clostridium sticklandii, clostridium aminophilum and peptostreptococcus anaerobius). other non-bacteriocin producing s. bovi ...200919171197
lysozyme and microbiota in relation to gingivitis and periodontitis.the aim of this study was to investigate associations between the immune component of the oral cavity, lysozyme, in gingival crevicular fluid and unstimulated saliva, and colonization dynamics of periodontopathogenic bacteria from supra- and subgingival plaque samples in patients with gingivitis or periodontitis.200919179970
antibiotic-resistant obligate anaerobes during exacerbations of cystic fibrosis patients.pseudomonas aeruginosa and staphylococcus aureus are thought to cause the majority of lung infections in patients with cystic fibrosis (cf). however, other bacterial pathogens may contribute to the pathophysiology of lung disease. here, obligate anaerobes were identified in a cross-sectional study, and cell numbers and antibiotic susceptibilities of facultative and obligate anaerobes from 114 sputum samples from nine children and 36 adults with cf were determined. furthermore, in 12 cf patients, ...200919196263
does diclofenac increase the risk of cervical necrotizing fasciitis in a rat model?non-steroidal anti-inflammatory drugs (nsaids) are known for aggravating in vitro infections and were reported in many cases of cervical necrotizing fasciitis (cnf). we developed a rat model of cnf, mimicking as closely as possible the human-cnf, to study the effect of a nsaids, diclofenac, as a promoting factor. twenty rats were injected bilaterally in the neck with peptostreptococcus and with a fresh saliva specimen for another 20 rats. half of each group was given an intramuscular injection o ...200919200252
microbial transformation and bioactivation of isoflavones from pueraria flowers by human intestinal bacterial strains.the flowers from pueraria, which are called puerariae flos, have been used since ancient times for recovery from alcohol intoxication. we elucidated the microbial transformation of the main isoflavones (1, 1a and 2) by using 29 commercially available human intestinal bacterial strains together with the bioactivation of the hepatoprotective activity of their metabolites. tectoridin (1a), which contains only one glucosyl moiety, was metabolized to its aglycone 1b by various bacterial strains. on t ...200919219523
the bacteriology of chronic rhinosinusitis with and without nasal compare the bacteriologic findings in ethmoidal biopsy specimens and nasal lavage samples from healthy control patients and from patients with chronic rhinosinusitis (crs) with nasal polyps (crsnp+) and without nasal polyps (crsnp-).200919221239
does pregnancy have an impact on the subgingival microbiota?we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.200919228092
[diabetic foot infections. prevalence and antibiotic sensitivity of the causative microorganisms].foot infections are a common reason for hospitalization and a cause of complications in patients with diabetes. the aim of this study was to determine the prevalence of microorganisms found on culture in complicated diabetic foot infections in hospitalized patients, and the sensitivity of the causative microorganisms to antimicrobial agents.200919237227
grafting of protein l-binding activity onto recombinant antibody fragments.recombinant antibody fragments consisting of variable domains can be easily produced in various host cells, but there is no universal system that can be used to purify and detect them in the free form or complexed with their antigen. protein l (ppl) is a cell wall protein isolated from peptostreptococcus magnus, which has been reported to interact with the v-kappa chain of some, but not all, antibodies. here we grafted the v-kappa framework region 1 (fr1) sequence of a high-affinity ppl-binding ...200919268418
clinical features and prognostic factors of anaerobic infections: a 7-year retrospective study.risk factors for mortality resulting from anaerobic infection are incompletely defined. the clinical significance of a broad range of pathogenic obligate anaerobic organisms was examined, and factors independently associated with mortality were identified in patients with clinically significant anaerobic infections.200919270476
into thin air. 200919272085
cytoprotective effect of proanthocyanidin-rich cranberry fraction against bacterial cell wall-mediated toxicity in macrophages and epithelial cells.recent studies brought evidence regarding the potential beneficial effects of cranberry polyphenols for periodontal infections. in this study, we evaluated the capacity of a proanthocyanidin-rich cranberry fraction to protect macrophages and oral epithelial cells against cytotoxicity induced by bacterial components. u937 cells, differentiated into adherent macrophage-like cells, as well as oral epithelial cells were treated with cell wall or lipopolysaccharide preparations from periodontopathoge ...200919277973
[septic shock caused by gardnerella vaginalis and peptostreptococcus species after cesarean section].a 41-year old multisectio patient got a life-threatening postoperative infection with gardnerella vaginalis and peptostreptococcus spp. perioperative treatment with cefuroxime and metronidazol is recommended.200919284925
anti-biofilm properties of satureja hortensis l. essential oil against periodontal pathogens.essential oils of several plants are widely used in ethnomedicine for their antimicrobial and anti-inflammatory properties. however, very limited data exist on their use in connection to periodontal diseases. the aim of the present study was to investigate the bacterial growth inhibiting and anti-biofilm effects of satureja hortensis l. (summer savory), salvia fruticosa m. (sage), lavandula stoechas l. (lavender), myrtus communis l., and juniperus communis l. (juniper) essential oils. chemical c ...200919285147
bacterial conversion of secoisolariciresinol and has been investigated whether secoisolariciresinol (seco) and anhydrosecoisolariciresinol (ahs), an acid degradation product of seco, could be fermented in a similar way, and to a similar extent, by members of the intestinal microbiota.200919302311
branchial cleft cysts in adults. diagnostic procedures and treatment in a series of 18 cases.branchial cleft anomalies may be presented as branchial cysts, fistulas, or sinuses. purpose of this paper is to present the diagnostic procedures and the treatment in a series of branchial cleft cysts.200919308468
clinical and microbiological determinants of ailing dental implants.the failure of the host tissue to establish or maintain osseointegration around dental implants is due to either occlusal or parafunctional forces, premature loading, ill-directed stress, or microbial infection. the long-term failure rate of dental implants is generally 5-10%. although a variety of etiologies of early peri-implant bone loss (from implant placement to 1-year post-loading) have been proposed, factors associated with late implant failures are less well understood but are probably r ...200918384407
photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial.recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ...200918465191
bacterial colonization of oral implants from nondental sources.implants showing signs of peri-implantitis harbor a microbiota similar to that of periodontitis-affected teeth. this case report describes the subgingival microbiota of a 45-year-old female with advanced periodontitis before and after complete edentulation and reconstruction with dental implants. a 3-month healing period post extraction passed before implants were placed using a two-stage submerged implant protocol. at 4- to 6-month recall visits after definitive prosthetic reconstruction, some ...200918657153
in vitro bactericidal activity of recombinant human beta-defensin-3 against pathogenic bacterial strains in human tooth root canal.human beta-defensin-3 (hbd3), an endogenous antimicrobial peptide, has strong broad-spectrum antimicrobial activity. this study aimed to obtain recombinant hbd3 (rhbd3) and to test the hypothesis that the antimicrobial characteristics of hbd3 may offer an advantage over conventional medicine in reducing intracanal bacteria. genetic engineering was used to obtain active rhbd3 and analysis revealed that it exhibited a broad spectrum of antibacterial activity at low micromolar concentrations agains ...200918775647
targeted antimicrobial activity of a specific igg-smap28 conjugate against porphyromonas gingivalis in a mixed culture.antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. to assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (smap) 28 was linked to affinity- and protein g-purified rabbit immunoglobulin g (igg) antibodies specific to the outer sur ...200918778918
pericarditis caused by anaerobic bacteria.this review describes the microbiology, diagnosis and management of pericarditis due to anaerobic bacteria. the predominant anaerobes isolated from patients with pericarditis are gram-negative bacilli (mostly bacteroides fragilis group) as well as peptostreptococcus, clostridium, fusobacterium, bifidobacterium and actinomyces spp. anaerobic bacteria can be recovered from pericarditis resulting from the following mechanisms: (i) spread from a contiguous site of infection, either de novo or follow ...200918789852
spondylodiscitis due to peptostreptococcus spp: a case report. 200919022696
sudden onset proptosis secondary to cavernous sinus thrombosis from underlying mandibular dental infection.the present report concerns a patient who presented with a 4-day history of left-sided facial pain arising from a pre-existing dental infection and progressive shortness of breath. the patient had a previous diagnosis of rheumatoid arthritis and was being treated with methotrexate. the rapid development of a right eye proptosis necessitated urgent decompression with a lateral canthotomy and cantholysis. imaging revealed a left facial abscess, cavernous sinus thrombosis (cst), bilateral internal ...200921789100
postoperative mediastinitis due to finegoldia magna with negative blood cultures.we report a case of finegoldia magna (formerly known as peptostreptococcus magnus) mediastinitis following coronary artery bypass in a 50-year-old patient. even if staphylococci remain the main causative organism of postoperative mediastinitis, the responsibility of anaerobic bacteria must be considered in cases of fever and sternal drainage with negative blood cultures.200919812272
comparative evaluation of the antimicrobial efficacy of aloe vera tooth gel and two popular commercial toothpastes: an in vitro study.aloe vera (aloe barbadensis miller) has been suggested for a wide variety of ailments but its use in dentistry is limited. this article reviews the uses of the plant and describes an in vitro investigation that compared the antimicrobial effectiveness of aloe vera tooth gel with two popular, commercially available dentifrices. the preliminary results showed that aloe vera tooth gel and the toothpastes were equally effective against candida albicans, streptococcus mutans, lactobacillus acidophilu ...200919819812
bacterial diversity in persistent periapical lesions on root-filled teeth.the purpose of this study was to analyze the bacterial diversity in persistent apical lesions on root-filled teeth by using culture-independent molecular methods.200921523213
tongue biofilm areal density and tongue coating index.two methods have been employed to measure the amount of tongue biofilm in humans: (1) areal density of microbes (cfu cm(-2)) (tongue scrape from a known/measured area), or (2) a tongue-coating index, where a human judge assesses the amount of 'coating' by visual inspection. the two methods were compared, both on human volunteers and on artificial biofilms. fusobacterium nucleatum (fn), prevotella intermedia (pi), streptococcus mutans (sm) and peptostreptococcus micros (pm) were obtained from sto ...200821386152
deep neck abscess: an analysis of microbial etiology and the effectiveness of antibiotics.the objective was to demonstrate the aerobic and anaerobic microbiology of deep neck space abscess and to analyze the coverage rate of different empiric antimicrobial agents. a retrospective review of hospitalized patients with deep neck abscess diagnosed at a tertiary-care, general hospital between april 2001 and october 2006. the study enrolled 100 patients. the bacterial cultures of 89 patients yielded positive results (89%). the predominant aerobes were viridans streptococci, klebsiella pneu ...200821694873
frequency, microbial interactions, and antimicrobial susceptibility of fusobacterium nucleatum and fusobacterium necrophorum isolated from primary endodontic infections.this study assessed the prevalence and microbial interactions of fusobacterium nucleatum and fusobacterium necrophorum in primary endodontic infections from a brazilian population and their antimicrobial susceptibility to some antibiotics by the e-test. one hundred ten samples from infected teeth with periapical pathologies were analyzed by culture methods. five hundred eighty individual strains were isolated; 81.4% were strict anaerobes. f. nucleatum was found in 38 root canals and was associat ...200819026872
the effect of a supragingival plaque-control regimen on the subgingival microbiota in smokers and never-smokers: evaluation by real-time polymerase chain reaction.the aim of the present study was to evaluate the effect of strict supragingival plaque control on the subgingival microbiota in smokers and never-smokers. research into the impact of supragingival plaque control on the number of subgingival bacteria has resulted in contradictory findings. real-time polymerase chain reaction (pcr) has been suggested as a valid alternative to current microbiologic methods based on bacteria cultures.200819053920
infective endocarditis caused by anaerobic bacteria.this review summarizes the microbiology, diagnosis and management of infective endocarditis (ie) due to anaerobic bacteria. anaerobic bacteria are an uncommon but important cause of ie. most cases are caused by the anaerobic and microaerophilic streptococci propionibacterium acnes, bacteroides fragilis and clostridium species. predisposing factors and signs and symptoms of ie caused by anaerobic bacteria are similar to those seen in ie due to facultative anaerobic bacteria with the following exc ...200819056073
anaerobic culture of diabetic foot infections: organisms and antimicrobial susceptibilities.the prevalence of diabetes mellitus is high in singapore. infections of the lower limb are significant causes of morbidity in this population. although the aerobic bacteriology of these infections is well-documented, there is less data available on the anaerobic pathogens involved. this study sets out to describe the anaerobic bacteria associated with diabetic foot infections, and evaluates the susceptibility to 3 antimicrobials with anaerobic activity.200819082200
[antibiotic therapy in odontogenic infections in children and adolescents: pharmacokinetic/pharmacodynamic analysis].the objective of this study was to evaluate the efficacy of the most commonly used antimicrobial treatments in odontogenic infections in children and adolescents on the basis of pharmacokinetic/ pharmacodynamic (pk/pd) criteria.200819100192
the role of anaerobic bacteria in chronic suppurative otitis media in children: implications for medical therapy.this review describes the microbiology, diagnosis and medical management of chronic suppurative otitis media (csom) in children highlighting the role of anaerobic bacteria. in studies that employed adequate method for recovery of anaerobic bacteria polymicrobial aerobic and anaerobic flora was isolated from over half of the children with csom. the predominant aerobic isolates were staphylococcus aureus and pseudomonas aeruginosa and the most frequently isolated anaerobic organisms were peptostre ...200819114112
relationship of specific vaginal bacteria and bacterial vaginosis treatment failure in women who have sex with women.bacterial vaginosis frequently persists after treatment. the role of newly defined bacterial vaginosis-associated bacteria (bvab), which have a specificity for this condition of 97% or greater, has not been assessed.200818591634
a case of necrotizing fasciitis due to streptococcus agalactiae, arcanobacterium haemolyticum, and finegoldia magna in a dog-bitten patient with diabetes.we report a case of necrotizing fasciitis involving streptococcus agalactiae, arcanobacterium haemolyticum, and finegoldia magna in a 36-yr-old female diabetic patient, which started after a minor dog bite to the toe of the patient. this case suggested that a trivial infection after a minor dog bite in an immunocompromised patient such as diabetes patient could result in a significant complication such as necrotizing fasciitis. the life-threatening infection was cured by timely above-the-knee am ...200818594170
increasing trends in antimicrobial resistance among clinically important anaerobes and bacteroides fragilis isolates causing nosocomial infections: emerging resistance to carbapenems.this study reports data on the susceptibilities to five commonly used antianaerobic agents of five clinically frequently encountered anaerobes from 2000 to 2007 and to bacteroides fragilis isolates causing nosocomial infections from 1990 to 2006. there was a trend of decreasing susceptibilities of these anaerobes to ampicillin-sulbactam, cefmetazole, chloramphenicol, and clindamycin with time during the study period. the rates of susceptibility to clindamycin and cefmetazole for all clinical iso ...200818625771
formulation and evaluation of pla and plga in situ implants containing secnidazole and/or doxycycline for treatment of periodontitis.the purpose of this study is to formulate in situ implants containing doxycycline hydrochloride and/or secnidazole that could be used in the treatment of periodontitis by direct periodontal intrapocket administration. biodegradable polymers [poly (lactide) (pla) and poly (lactide-co-glycolide) (plga)], each polymer in two concentrations 25%w/w, 35%w/w were used to formulate the in situ implants. the rheological behavior, in vitro drug release and the antimicrobial activity of the prepared implan ...200818654864
the role of toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens.periapical lesions arise as a result of the activation and interaction of the host immune responses against root canal infection. recently identified toll-like receptors (tlr) seem to be involved in the recognition and development of immune responses against a myriad of microorganisms. however, very little information is available on the role of tlr in the induction of periapical lesions.200818793356
rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by facilitate the identification of anaerobes cultivated from periodontal disease, whole cell bacterial identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) was evaluated.200818793359
molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain was the aim of the present study to evaluate root canal samples for the presence and numbers of specific species as well as for total bacterial load in teeth with chronic apical periodontitis using quantitative real-time polymerase chain reaction (pcr).200818793361
orbital abscess secondary to acute dacryocystitis: case report.acute dacryocystitis usually induces preseptal infection. in rare instances the infection that is confined to the lacrimal sac can extend to the orbital contents resulting in orbital cellulitis. we present a case of intraconal abscess secondary to acute dacryocystitis and review the literature of orbital cellulitis resulting from acute lacrimal sac infection.200818797671
bacteriological and clinical evaluation of twelve cases of post-surgical sepsis of odontogenic tumours at a referral determine the bacterial aetiology of sepsis occurring following surgery of odontogenic tumours and assess the effect of prompt and proper antibiotic usage.200818817023
rationally designed ligands for use in affinity chromatography: an artificial protein l.synthetic affinity ligands can circumvent the drawbacks of natural immunoglobulin (ig)-binding proteins by imparting resistance to chemical and biochemical degradation and to in situ sterilization, as well as ease and low cost of production. protein l (ppl), isolated from peptostreptococcus magnus strains, interacts with the fab (antigen-binding fragment) portion of igs, specifically with kappa light chains, and represents an almost universal ligand for the purification of antibodies. the concep ...200818826050
polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded flx amplicon pyrosequencing (btefap).diabetic extremity ulcers are associated with chronic infections. such ulcer infections are too often followed by amputation because there is little or no understanding of the ecology of such infections or how to control or eliminate this type of chronic infection. a primary impediment to the healing of chronic wounds is biofilm phenotype infections. diabetic foot ulcers are the most common, disabling, and costly complications of diabetes. here we seek to derive a better understanding of the pol ...200818833331
correlates of periodontal decline and biologic markers in older adults.there is limited information on infectious and host responses distinguishing older people with or without active periodontitis. this study measured bacterial and serum cytokine and high-sensitivity c-reactive protein (hscrp) levels in older persons.200818834247
purulent pericarditis with cardiac tamponade caused by anaerobic agents.purulent pericarditis is a rare disease associated with high mortality, even with appropriate antibiotic therapy. the authors report the case of a patient with purulent pericarditis with no identifiable primary source that presented clinically as cardiac tamponade. the patient required emergency pericardiocentesis and surgical pericardiectomy due to persisting signs of tamponade. microbiology cultures isolated anaerobic agents of fusobacterium spp., corynebacterium spp. and peptostreptococcus. t ...200818959092
alternate arrangement of ppl b3 domain and spa d domain creates synergistic double-site binding to vh3 and vkappa regions of our previous study, a kind of novel hybrid immunoglobulin (ig)-binding proteins (ibps) was obtained with the characteristic structure of alternately arranged finegoldia magna (formerly peptostreptococcus magnus) protein l (p. magnus protein l, ppl) b3 domain (b3) and staphylococcal protein a (spa) d domain (d). in this study, two representative molecules of these novel proteins, ld3 (b3-d-b3) and ld5 (b3-d-b3-d-b3) (ld3/5), showed substantially higher affinity for igg-f(ab')2, igm, and iga th ...200818694300
value of anaerobic culture in bacterial surveillance program for platelet concentrates.short-term aerobic bacterial culture (stabc) has been used routinely in hong kong since 1998 to reduce bacterial contamination in platelet concentrates (pcs) with good results. with more countries implementing routine aerobic and anaerobic cultures of pcs, a prospective study was conducted to determine the value of anaerobic culture to stabc.200818694462
evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (ig)-binding proteins (ibps) with four kinds of ig molecules.protein a, protein g and protein l are three well-defined immunoglobulin (ig)-binding proteins (ibps), which show affinity for specific sites on ig of mammalian hosts. although the precise functions of these molecules are not fully understood, it is thought that they play an important role in pathogenicity of bacteria. the single domains of protein a, protein g and protein l were all demonstrated to have function to bind to ig. whether combinations of ig-binding domains of various ibps could exh ...200818700046
clinicopathologic challenge. gangrenous cellulitis caused by peptostreptococcus. 200818717855
the influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice.this study assessed the influence of mineral trioxide aggregate (mta) on adaptive immune responses. balb/c mice were immunized with heat-killed fusobacterium nucleatum (fn) in mta or other control adjuvants, and serum igg responses to fn were measured. either fn- or peptostreptococcus anaerobius (pa)-reactive memory t cells (tm) were preincubated in vitro with/without mta and restimulated with fn or pa. tm proliferation and cytokine production were assessed. compared with control groups, immunog ...200818718367
detection of anaerobic prosthetic joint infection by pcr and dna sequencing--a case report. 200818766493
bisphosphonate-associated osteonecrosis: a clinician's reference to patient management.bisphosphonates (bps), as inhibitors of osteoclasts, are widely used in the management of metastatic bone disease and in the prevention of osteomalacia and osteoporosis. recent cases of bone necrosis of the jaws have been associated with the use of bisphosphonate therapy. a case is presented of a patient with osteonecrosis of the maxilla with a history of long-term bisphosphonate therapy for metastatic breast cancer. the authors treated the patient and suggest appropriate patient management guid ...200818767452
root canal microbiota of teeth with chronic apical periodontitis.samples from infected root canals of 43 teeth with chronic apical periodontitis were analyzed for the presence and relative levels of 83 oral bacterial species and/or phylotypes using a reverse-capture checkerboard hybridization assay. associations between the most frequently detected taxa were also recorded. the most prevalent taxa were olsenella uli (74%), eikenella corrodens (63%), porphyromonas endodontalis (56%), peptostreptococcus anaerobius (54%), and bacteroidetes oral clone x083 (51%). ...200818768651
[peptostreptococcus endocarditis: presentation of two cases and literature review].we describe two new cases of peptostreptococcus endocarditis, one case of peptostreptococcus micros prosthetic valve endocarditis and the other of peptostreptococcus assaccharolyticus tricuspid native valve endocarditis in an intravenous drug user (idu) patient and review nine cases previously reported.200818769744
photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial.recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment.200818771363
campylobacter species isolated from extra-oro-intestinal abscesses: a report of four cases and literature review.campylobacter species are frequently isolated from fecal specimens of patients with diarrheal illness. several campylobacter species are commonly isolated from the oral cavity. in contrast, campylobacter species are rarely isolated from extra-oro-intestinal abscesses. reported here are four cases of extra-oro-intestinal abscesses due to polymicrobial flora, including campylobacter species. the first case is a 35-year-old woman who was diagnosed with a brain abscess caused by c. gracilis, strepto ...200818488257
changes in abundance of lactobacillus spp. and streptococcus suis in the stomach, jejunum and ileum of piglets after weaning.this present study investigated the changes in bacterial community composition, with an emphasis on lactobacillus spp. and streptococcus suis populations as potentially beneficial and harmful groups, in the stomach, jejunum and ileum of piglets after weaning (21 days postpartum) by 16s rrna gene-based methods. denaturing gradient gel electrophoresis analysis showed that, after weaning, predominant bands related to lactobacillus spp. disappeared and were replaced by potential pathogenic species, ...200818554303
influence of local tetracycline on the microbiota of alveolar osteitis in rats.the aim of the present study was to evaluate the effects of local tetracycline on the occurrence of alveolar osteitis in rats, and on the microbiota associated to this infection. forty wistar rats were randomly assigned to 4 groups (n=10): i - the rats had the maxillary right incisor extracted and the alveolar wound did not receive any treatment; ii - adrenaline and ringer-pras were introduced into the alveolar wound; iii - the alveolar wound was irrigated with sterile saline; and iv - the alveo ...200818568225
quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems.transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ...200818579722
deep brain stimulation for holmes' tremor related to a thalamic abscess.medical treatment is usually ineffective for holmes' tremor, and surgery is the treatment of choice for many patients. here we report the case of a 14-year-old girl who developed holmes' tremor related to a thalamic abscess and was successfully treated with thalamic deep brain stimulation.200818581123
microbiology and management of joint and bone infections due to anaerobic describes the microbiology, diagnosis, and management of septic arthritis and osteomyelitis due to anaerobic bacteria.200818392922
ocular infections due to anaerobic bacteria in children.the recent increased recovery of anaerobic bacteria from children has led to greater appreciation of the role these organisms play in pediatric infections at all body sites, including the eye. in studies that employed adequate methods for recovery of anaerobes, they were isolated from approximately one-third of the children with conjunctivitis, half of the time in pure culture. the predominant recovered anaerobes were clostridium spp., gram-negative anaerobic bacilli, and peptostreptococcus spp. ...200818404954
[microbiological and biochemical characteristics of inflammatory tissues in the periodontium].to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva.200818413987
infection associated with hematoma formation after shoulder arthroplasty.hematoma formation requiring operative treatment after shoulder arthroplasty may be associated with higher patient morbidity. we therefore determined whether there was an association of hematoma formation requiring operative treatment with deep infection after shoulder arthroplasty. between 1978 and 2006, we performed 4147 shoulder arthroplasties in 3643 patients. of these, 12 shoulders (0.3%) underwent reoperation for hematoma formation. the mean time interval from arthroplasty to surgery for t ...200818421541
microbial analysis of canals of root-filled teeth with periapical lesions using polymerase chain reaction.the objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. dna was extracted from 45 canal samples of root-filled teeth with periapical lesions. a pcr assay using species-specific primers of 16s rdna and the downstream intergenic spacer region was used for microbial detection. enterococcus faecalis ...200818436030
urea and guanidinium chloride denature protein l in different ways in molecular dynamics performing protein-denaturation experiments, it is common to employ different kinds of denaturants interchangeably. we make use of molecular dynamics simulations of protein l in water, in urea, and in guanidinium chloride (gdmcl) to ascertain if there are any structural differences in the associated unfolding processes. the simulation of proteins in solutions of gdmcl is complicated by the large number of charges involved, making it difficult to set up a realistic force field. furthermore, at ...200818339753
moraxella catarrhalis-dependent tonsillar b cell activation does not lead to apoptosis but to vigorous proliferation resulting in nonspecific igm production.the respiratory pathogen moraxella catarrhalis has a high affinity for human igd and is mitogenic for peripheral blood b lymphocytes. moraxella igd-binding protein, which is a multifunctional outer membrane protein with adhesive properties, is responsible for the interaction. previous experiments with the ig-binding b cell superantigens protein a and protein l from staphylococcus aureus and peptostreptococcus magnus, respectively, have suggested that nonimmune bcr cross-linking induces b cell ap ...200818372337
[lumbar pyomyositis caused by finegoldia magna (peptostreptococcus magnus) in a patient with hepatitis c virus infection]. 200818373916
a retrospective analysis of isolates from patients with vaginitis in a private greek obstetric/gynecological hospital (2003-2006).vaginitis is a common cause of complaints in women in various parts of the world. through our literature search, we identified noteworthy differences in the etiology of vaginitis based on the geographic location. this study was conducted to describe microbial etiology in a population of symptomatic women with vaginitis in greece.200818376352
generation and expression of a minimal hybrid ig-receptor formed between single domains from proteins l and g.the ig-binding properties of protein l from peptostreptococcus magnus and protein g from streptococcus have been successfully combined through the construction of a novel hybrid protein, consisting of a single ig-binding domain from each protein. the biophysical and biochemical properties of this construct have been characterized through equilibrium and pre-equilibrium fluorescence spectroscopy, circular dichroism, isothermal titration calorimetry, affinity chromatography, and conformational sta ...200818158250
european surveillance study on antimicrobial susceptibility of gram-positive anaerobic cocci.gram-positive anaerobic cocci (gpac) are a heterogeneous group of microorganisms frequently isolated from local and systemic infections. in this study, the antimicrobial susceptibilities of clinical strains isolated in 10 european countries were investigated. after identification of 299 gpac to species level, the minimum inhibitory concentrations of penicillin, imipenem, clindamycin, metronidazole, vancomycin and linezolid were determined by the agar dilution method according to the clinical and ...200818180149
detection and identification of bacterial dna in detect and identify bacteria in semen by sequencing polymerase chain reaction (pcr)-amplified ribosomal rna gene regions (rdnas).200818191853
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