TitleAbstractYear(sorted ascending)
the primary structure of peptostreptococcus elsdenii flavodoxin. 19734711610
effect of acetohydroxamic acid on growth and volatile fatty acid production by rumen bacteria. 19734734379
studies on the kinetics and mechanism of reduction of flavodoxin from peptostreptococcus elsdenii by sodium dithionite. 19734743900
anaerobic microflora of the cervix in healthy women. 19734758303
dependence of the proton magnetic resonance spectra on the oxidation state of flavodoxin from clostridium mp and from peptostreptococcus elsdenii.the broadening of protein nuclear magnetic resonances in the spectra of the semiquinone forms of flavodoxins derived from clostridium mp and peptostreptococcus elsdenii relative to the resonances in the oxidized and reduced forms is highly selective. spectra from both species of flavodoxin indicate that conformational differences between the oxidized and fully reduced states are minor and, consequently, the broadening in the semiquinone form is ascribed to the paramagnetic effect of the flavin f ...19734519623
[treatment of bacteroides infections with lincomycin]. 19734543418
anaerobic bacteria in oral infections. 19744528419
the effects of preoperative rinsing with cetylpyridinium chloride on bacteremia associated with the surgical removal of impacted third molars. 19744528780
liver abscess production by non-spore-forming anaerobic bacteria in a mouse model.a model anaerobic infection consisting of progressive intrahepatic abscesses was produced in mice by injection of single and combined species of non-spore-forming anaerobic bacteria.19744816634
childhood cervical lymphadenitis: a reappraisal. 19744826618
parenteral clindamycin therapy for severe anaerobic infections. 19744833936
correction of the amino acid sequence of peptostreptococcus elsdenii flavodoxin. 19744843143
significance of anaerobic bacteria isolated from the urinary tract. i. clinical studies. 19744843341
tryptophan biosynthesis from indole-3-acetic acid by anaerobic bacteria from the rumen.microbes in ruminal contents incorporated (14)c into cells when they were incubated in vitro in the presence of [(14)c]carboxyl-labeled indole-3-acetic acid (iaa). most of the cellular (14)c was found to be in tryptophan from the protein fractions of the cells. pure cultures of several important ruminal species did not incorporate labeled iaa, but all four strains of ruminococcus albus tested utilized iaa for tryptophan synthesis. r. albus did not incorporate (14)c into tryptophan during growth ...19744855566
predominant gram-positive bacteria in human feces: numbers, variety, and persistence.the predominant gram-positive bacteria in 47 fecal specimens from 10 healthy men were studied by microscopic and cultural counts, by the characterization and tentative identification of isolates, and by the use of fluorescein isothiocyanate (fitc)-conjugated globulins prepared using some of the isolates. gram-positive bacteria averaged 10(10.5+/-0.4(sd)/g (wet weight) of feces with significant variation from host to host. characterization of 865 isolates, all strict anaerobes and carbohydrate fe ...19744595760
isolation, culture characteristics, and identification of anaerobic bacteria from the chicken cecum.studies on the anaerobic cecal microflora of the 5-week-old chicken were made to determine a suitable roll-tube medium for enumeration and isolation of the bacterial population, to determine effects of medium components on recovery of total anaerobes, and to identify the predominant bacterial groups. the total number of microorganisms in cecal contents determined by direct microscope cell counts varied (among six samples) from 3.83 x 10(10) to 7.64 x 10(10) per g. comparison of different nonsele ...19744596749
isolation and indentification of anaerobic bacteria in the clinical laboratory. a 2-year experience. 19744597783
bacteremia after tooth extractions studied with the aid of prereduced anaerobically sterilized culture media.both prereduced molten agar and broth and aerobic molten agar and broth were inoculated with blood samples collected from patients with periodontitis, but in otherwise good health, both before and after extraction of two or more teeth. postoperative blood samples from 23 of 25 patients sampled yielded anaerobic and facultative species. colony counts from nine samples yielded from less than 1 to over 100 colonies per ml of blood. organisms detected were species belonging to the genera bacteroides ...19744598226
sodium polyanethol sulfonate sensitivity of anaerobic cocci.growth of peptostreptococcus anaerobius was shown to be totally inhibited by sodium polyanethol sulfonate (sps). other anaerobic cocci grew in the presence of sps although some strains of peptococcus prevotii and peptococcus magnus showed delayed growth. a sps disk assay for the presumptive identification of p. anaerobius is described.19744598436
clindamycin in pure and mixed anaerobic infections. 19744600682
the bacteriology of intra-abdominal infections. 19744605216
anterior cervical infections: complications of transtracheal aspirations. 19744606222
studies on the cecal microflora of commercial broiler chickens.a study was made of the cecal microflora isolated from broilers (5-week-old) reared under typical commercial husbandry conditions. three hundred and twenty-five bacterial strains (randomly isolated from colonies representing 49 to 81% of the microscopic count) were isolated from cecal digesta of six animals on a rumen fluid roll tube medium (m98-5). seventy-seven percent of these strains consisted of strict anaerobes: gram-negative, pleomorphic cocci (5.2%), peptostreptococcus (1.5%), gram-posit ...19744608322
mycotic aneurysms. 19744479737
extracellular deoxyribonuclease production by anaerobic bacteria.the production of extracellular deoxyribonuclease was examined with anaerobic organisms isolated from clinical specimens. nuclease activity was extraordinarily common. all strains of fusobacterium, including eight species, as well as bacteroides fragilis and b. melaninogenicus, displayed enzyme activity. whereas the gram-positive bacteria were generally less productive, all strains of clostridium perfringens, peptostreptococcus intermedius, and p. anaerobius specifically produced deoxyribonuclea ...19744364608
effect of culture medium and carbon dioxide concentration on growth of anaerobic bacteria commonly encountered in clinical specimens.representative strains of anaerobic bacteria from human infections were used to evaluate broth media, gas mixtures, and inocula for use in developing a procedure for performing minimal inhibitory concentration antimicrobic susceptibility tests. nine commercially available media, including two that were chemically defined, were tested. tests were performed in atmospheres with carbon dioxide concentrations between 2.5 and 10% and also in the gaspak system (bbl) that had a disposable hydrogen-carbo ...19744364610
identification and properties of new flavins in electron-transferring flavoprotein from peptostreptococcus elsdenii and pig-liver glycolate oxidase. 19744365840
the isolation of anaerobic bacteria from wound swabs.the isolation of anaerobic bacteria from routine wound swabs by three procedures was evaluated. recovery of anaerobic organisms was doubled by immediate incubation of seeded plates, and the recovery could be further dramatically improved by the use of prereduced media, in conjunction with an anaerobic chamber. recommendations for the treatment of swabs and cultures for anaerobic investigation are made.19744371159
bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils.human polymorphonuclear neutrophils (pmn) were made anaerobic by nitrogen washout (oxygen saturation <1%, eh < -42 mv at ph 7.0), and the ability of the cells to kill bacteria was assayed and compared to the bactericidal activity of aerobic pmn. anaerobic pmn were able to kill staphylococcus epidermidis, enterococcus, viridans streptococci, pseudomonas aeruginosa, peptostreptococcus anaerobius, bacteroides fragilis, clostridium perfringens, and peptococcus magnus normally. organisms that were no ...19744361295
phosphatase activity of anaerobic organisms.anaerobic organisms were tested for phosphatase activity in different ph ranges. several groups of organisms displayed characteristic patterns. bacteroides fragilis, b. melaninogenicus, and b. ruminicola produced phosphatase with strongest activity at ph 8.6. fusobacterium mortiferum was the only species of this genus to show strong hydrolysis. the enzyme was active in both acid and alkaline ranges. the activity of gram-positive organisms was variable, the most active groups being clostridium pe ...19744363556
purification and properties of electron-transferring flavoprotein from peptostreptococcus elsdenii. 19744364030
microbial flora found in the products of conception in spontaneous abortions. 19744203014
stomach fermentation in east african colobus monkeys in their natural state.the microbial fermentation in the stomachs of two monkeys, colobus polykomos, collected in kenya, was studied. the gas accumulated within the stomach contained h(2) but no ch(4). volatile fatty acid concentrations were high, but accumulated acid prevented determination of the fermentation rate in untreated, incubated stomach contents. upon addition of bicarbonate, a very rapid rate could be demonstrated. some d- and l-lactate were in the stomach contents. starchy seeds or fruits rather than leav ...19744207763
n-terminal amino acid sequences of azotobacter vinelandii and rhodospirillum rubrum flavodoxins. 19744212186
isolation of ureolytic peptostreptococcus productus from feces using defined medium; failure of common urease tests.colony counts of fecal samples from three persons, obtained by using a chemically defined anaerobic roll-tube medium (containing glucose, maltose, glycerol, minerals, hemin, b-vitamins, methionine, volatile fatty acids, sulfide, bicarbonate, agar, carbon dioxide (gas phase), and 1 mm nh(4) (+) as main nitrogen source), averaged 60% of the 8.8 x 10(10) bacteria per g obtained when 0.2% trypticase and 0.05% yeast extract were added to the otherwise identical medium. when 0.2% vitamin-free casitone ...19744213672
synergistic necrotizing myositis complicating vascular injury. 19744214303
[changes of susceptibility of anaerobes to various antibiotics (author's transl)]. 19744215896
nutritional features and ecology of predominant anaerobic bacteria of the intestinal tract. 19744217101
establishment of a limited rumen flora in gnotobiotic lambs fed on a roughage diet. 19744217352
[subphrenic abscess due to peptostreptococcus]. 19744219409
whipple disease: light and electron microscopy study. 19744138040
fluorescent antibody study of the gram-positive anaerobic cocci.fluorescent antibody conjugates were prepared from five species of anaerobic cocci commonly isolated from human infections. when tested with homologous and heterologous cells these conjugates were found to be highly specific. there was no evidence of a common genus antigen. peptococcus magnus conjugates detected a species-specific antigen; cross-reactions with peptostreptococcus anaerobius were readily eliminated by absorption. the conjugates from peptococcus asaccharolyticus, peptococcus prevot ...19744140708
evidence that apo-reduced nicotinamide adenine dinucleotide dehydrogenase and apo-electron-transferring flavoprotein from peptostreptococcus elsdenii are identical. 19744151307
[experiences with freter's anaerobic chamber in the clinical laboratory]. 19744154683
micro-organisms from necrotic pulp of traumatized teeth. 19744155793
[susceptibility of non-sporing anaerobes of the genera bacteroides, fusobacterium, sphaerophorus, peptococcus, and peptostreptococcus to clindamycin (author's transl)]. 19744155889
[bacteriological investigations on reptiles and amphibians (author's transl)]. 19744155899
d-lactate dehydrogenase of peptostreptococcus elsdenii.d-lactate dehydrogenase has been purified to near homogeneity from peptostreptococcus elsdenii. as isolated, the enzyme contains flavine adenine dinucleotide and a tightly bound metal cofactor. inactivation by ortho-phenanthroline occurs in two steps and is partially blocked by d-lactate. reactivation by divalent metal ions occurs, with divalent zinc being the most effective. when ferricyanide is used as the electron acceptor, d-lactate has an apparent k0.5 of 3.3 m0.46; its binding is negativel ...1975368
purification, new assay, and properties of coenzyme a transferase from peptostreptococcus elsdenii.coenzyme a (coa) transferase from peptostreptococcus elsdenii has been purified and crystallized, and some of its properties have been established. the work was facilitated by a newly developed coupled and continuous spectrophotometric assay in which the disappearance of added acrylate could be followed at 245 nm. the rate-limiting conversion of acetyl- and beta-hydroxypropionyl coa to acrylyl coa by coa transferase was followed by the non-rate-limiting conversion to beta-hydroxypropionyl coa by ...1975369
susceptibility of anaerobic bacteria to metronidazole: relative resistance of non-spore-forming gram-positive baccilli.susceptibility of 358 clinical isolates of obligate anaerobes to metronidazole was determined by an agar-dilution technique. only 66% of all isolates were inhibited by 6.25 mug/ml, whereas 30% required larger than or equal to 50 mug/ml. considerable variation in susceptibility was observed among different genera and species of bacteria. fusobacterium was most senstitive, followed by clostridium, bacteroides and peptococcus, peptostreptococcus, veillonella and acidaminococcus, and non-spore-formi ...1975163867
prophylactic antibiotics in cesarean section.using a random selection procedure, 100 patients were evaluated in order to determine the value of prophylactic antibiotics in cesarean section. all patients were evaluated for febrile morbidity, and specific infection sites were recorded. significant differences were found between the treated and control groups and between cesarean sections performed prior to the onset of labor and after the onset of labor.1975164642
antibiotic susceptibility testing of anaerobic bacteria by the standardized disc diffusion method with special reference to bacteroides fragilis.the susceptibility of 90 strains of bacteroides fragilis to 16 antibacterial drugs was determined by the agar plate dilution test and the disc diffusion test. good correlation of results was obtained with the two methods, and regression line analysis could be performed for beta-lactam antibiotics, erythromycin, tetracyclines, lincomycin and clindamycin. thus the standardized disc diffusion method can be used for susceptibility testing, but only a clear distinction between sensitive and resistant ...1975167431
anaerobic infections of the female genital tract.anaerobic bacterial organisms are a frequent cause of female genital tract infections. there has been a lack of appreciation of the significant role anaerobes play in obstetric and gynecologic infections. utilization of newer appropriate anaerobic culture techniques has shown the importance of these organisms as pathogens in serious infections of the genital tract. many species of anaerobic bacteria appear to be part of the normal microflora of the lower genital tract in female subjects. similar ...1975167582
growth curves of anaerobic bacteria in solid media.simple pour plate and spectrophotometric techniques for the evaluation of growth curves of several anaerobic bacteria on solid media are described. three basic patterns of anaerobic growth were observed. the curves obtained were very reproducible when studied on separate occasions. the curves obtained by spectrophotometric measurement were comparable to those obtained by the pour plate method, especially when a large bacterial inoculum was used. limitations in the interpretation of the results a ...1975167660
electron-transferring flavoprotein of peptostreptococcus elsdenii that functions in the reduction of acrylyl-coenzyme peptostreptococcus elsdenii, a three-component flavoprotein electron transfer system catalyzes the oxidation of lactate and the reduction of crotonyl-coenzyme a (coa). spectral evidence showed that d-lactate dehydrogenase, when reduced by d-lactate, was able to reduce butyryl-coa dehydrogenase, but only in the presence of the electron-transferring flavoprotein. reduced nicotinamide adenine dinucleotide could replace reduced d-lactate dehydrogenase. a reconstituted system, containing the three ...1975172488
role of anaerobic bacteria in subdural empyema. report of four cases and review of 327 cases from the english literature.anaerobic bacteria were isolated from the subdural space in all four cases of subdural empyema encountered over a 2 and a half year period. only one aerobe was isolated in these cases. the bacteriology of subdural empyema was further analyzed from a review of 327 cases reported in the english literature. anaerobes accounted for 12 per cent of 234 cases; in addition, 27 per cent of cases were reportedly "sterile." these data support our finding that anaerobic bacteria may play a far more importan ...1975234678
[new species of lactic acid bacteria, described in the ussr, in the light of modern identification schemes]. 1975235331
d-lactate dehydrogenase of peptostreptococcus elsdenii. 1975236453
evaluation of bacteriological transport commercially manufactured bacteriological transport systems, including culturette, trans-cul (with stuart and amies), handiswab, securline (with amies and amies without charcoal) and culture caddy, were evaluated to determine whether these systems were capable of maintaining the viability and constant numbers of mixtures of hardy, fastidious and anaerobic organisms over 72 hours at 25c and 4c. the results of the study indicated that if specimens are maintained in transport systems at 25c t ...1975239597
survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems.the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ...1975240870
brain stem abscess treated surgically. wtih special note upon the employment of thorium dioxide.a 10-year-old girl, with congenital heart disease, harboring a brain stem abscess, was recently treated at the ucla hospital. needle aspirations of the abscess was performed through a posterior occipital craniectomy, and thorium dioxide (thorotrast) was placed within the abscess cavity as a marker. postoperatively, the patient improved temporarily but died 18 days later. autopsy examination included radioactive analysis of brain and liver tissue. radioautographs were superimposed on h&e preparat ...19751124486
[a case of empyema due to an anaerobic bacteria, peptostreptococcus]. 19751127834
disk susceptibility testing of slow-growing anaerobic bacteria.the susceptibility of 55 strains of slow-growing anaerobes to eight clinically useful or potentially useful antibiotics was determined by agar dilution and disk diffusion tests. strains of the genera peptococcus, peptostreptococcus, megasphaera, veillonella, eubacterium, bifidobacterium, clostridium, and fusobacterium were included. all strains were susceptible to chloramphenicol, but varied in their susceptibility to penicillin, lincomycin, clindamycin, tetracyclines, and vancomycin. correlatio ...19751137353
comparative susceptibility of anaerobic bacteria to minocycline, doxycycline, and tetracycline.the comparative susceptibility of 622 recent clinical isolates of anaerobic bacteria to minocycline, doxycycline, and tetracycline was determined by an agar-dilution technique. in addition to bacteroides fragilis, a variety of other anaerobic bacteria was resistant to achievable blood concentrations of tetracycline (55% inhibited by 6.25 mug/ml) and doxycycline (58% inhibited by 2.5 mug/ml). in contrast, minocycline was significantly more active (p < 0.05) than both doxycycline and tetracycline, ...19751137358
microdilution technique for antimicrobial susceptibility testing of anaerobic bacteria.a microdilution technique using commercially available media and materials was developed and used to determine the minimal inhibitory concentrations (mics) of clindamycin, chloramphenicol, tetracycline, minocycline, ampicillin, carbenicillin, cephalothin, and gentamicin for 101 anaerobic isolates. representative strains of bacteroides, clostridium, fusobacterium, peptococcus, and peptostreptococcus were tested. the use of schaedler broth at ph 7.2, an inoculum of 10(5) to 10(7) colony-forming un ...19751137383
acute pelvic inflammatory disease and clinical response to parenteral doxycycline.the bacteriology of acute pelvic inflammatory disease (pid) and clinical response to parenteral doxycycline were evaluated in 30 patients. only 3 of 21 cul-de-sac cultures from pid patients were sterile, whereas all 8 normal control subjects yielded negative results (p< 0.005). poor correlation was observed between cervical and cul-de-sac cultures. neisseria gonorrhoeae, isolated from the cervix in 17 patients (57%), was recovered from the cul-de-sac only once. streptococcus, peptococcus, peptos ...19751169908
bacteriology of normal and infected conjunctiva.the conjunctivas of 273 inflamed eyes were cultured by both aerobic and anaerobic techniques. isolations were obtained from 267 (97.8%) of the eyes. aerobic organisms were isolated from 237 (86.8%) of the conjunctivas. staphylococcus aureus from 63 (23.1%) of the diseased eyes was the aerobic pathogen most often isolated. anaerobic bacteria were isolated from 172 (63.0%) of the conjunctivas. propionibacterium acnes was isolated from 126 (46.2%) of the conjunctivas, and peptostreptococcus species ...19751176598
oxygen tolerance of fresh clinical anaerobic bacteria.the oxygen tolerance and sensitivity of 57 freshly isolated anaerobic bacteria from clinical specimens was studied. all the organisms tolerated 8 h or more of exposure to oxygen in room air. growth of the isolates in increasing oxygen concentrations demonstrated that the 57 isolates varied in oxygen sensitivity from strict to aerotolerant anaerobes. comparison of the oxygen tolerance and sensitivity showed that the most tolerant organisms (best survival after prolonged exposure) included anaerob ...19751176601
the reduction of n-hydroxy-4-acetylaminobiphenyl by the intestinal microflora of the rat.the role of the intestinal flora in the conversion of n-hydroxy-4-acetyl-aminobiphenyl (n-oh-aabp) to 4-acetylaminobiphenyl has been examined. this reaction, which reverses the metabolic activation of the parent carcinogen, can be demonstrated in cultures of some bacteria indigenous to the intestinal microflora. these include cultures of clostridium sp., clostridium perfringens, peptostreptococcus productus i, and bacteroides fragilis ss. thetaiotaomicron and ss. vulgatus. in contrast, cultures ...19751182690
"bad breath": presenting manifestation of anaerobic pulmonary infection.three patients are described in whom a putrid breath odor was the only or fist manifestation of anaerobic infection of the lung. fever, productive cough, and pleuritic pain, common findings in anaerobic pulmonary infection, were not initally present. "bad breath" may be an early due to the presence of anaerobic pulmonary infection.19751203000
the importance of the anaerobic bacteria in paranasal sinusitis.the anaerobic examination of the material, obtained by 100 antral punctures, in 66 patients with paranasal sinusitis, showed the importance of the anaerobic bacteria. in 33% we found anaerobes: 12% of the cultures were pure anaerobic, 21% were mixed aerobic-anaerobic. the most frequent anaerobe is the peptostreptococcus. anaerobic cultures are more frequently found in the unilateral cases of paranasal sinusitis. the presence of anaerobes does not seem to be dependent on the patient's age, the du ...19751224138
polymicrobial etiology of acute pelvic inflammatory disease.we studied 204 women with acute pelvic inflammatory disease to delineate further the causes of that illness. gonococci were recovered from 91. gonococcal pili antibody rose or fell significantly in 12 of 18 patients with positive cultures and only two of 19 who had negative cultures and smears for neisseria gonorrhaoea(p smaller than 0.005). n. gonorrhoeae was found in peritoneal exudate from eight of 21 patients with, and none of 33 without, cervical gonococcal infection. among patients with se ...1975806017
prevalence and antibiotic susceptibility of organisms isolated from acute soft-tissue abscesses secondary to dental caries. 1975806046
the bacteriology of acute pelvic inflammatory disease.the bacteriology of acute pelvic inflammatory disease (pid) was studied in 20 patients by simultaneous cul-de-sac, cervical, and blood cultures with aerobic and fastidious anaerobic techniques. cul-de-sac cultures were positive in 18 of 20 patients, while all 8 normal control subjects yielded negative results (p less than 0.0005, chi-square analysis). anaerobic bacteria (predominatly peptococcus and peptostreptococcus) were present in 10 patients (59 per cent), and they were the exclusive isolat ...1975807109
antibiotic treatment of oral anaerobic infections. 19751056466
puerperal endometritis: a prospective microbiologic study.this study was undertaken to determine the flora in the endometrial cavity of two groups of puerperal patients: 47 afebrile control subjects and 27 with endometritis. a transcervical culturing technique designed to avoid cervical contamination and to preserve aerobes, anaerobes, and mycoplasma was employed, and bacterial colony counts were reported semiquantitatively. no difference was found in the flora of these two groups. furthermore, the most common pathogenic organisms (in decreasing order ...19751090177
anaerobic infections of the female genital tract: prospects and perspectives. 19751094357
occurrence of megasphaera elsdenii in faecal samples of young pigs.important numbers of megasphaera elsdenii, known as a normal inhabitant of the rumen of cattle and sheep were recently cultured from intestinal contents of man and pigs. studying the occurrence of this organism in faecal contents of pigs, we paid attention to the use of a strict anaerobic isolating and counting technique. it was demonstrated that megasphaera elsdenii is a strict anaerobic organism. exposure to air during 15 minutes caused an important decrease of the colony count. comparison of ...19751098324
anaerobic specimen transport device.a device is described and evaluated for the anaerobic transport of clinical specimens. the device limits the amount of oxygen entering with the sample to a maximum of 2%, which is rapidly removed by reacting with hydrogen in the presence of a palladium catalyst. the viability on swabs of 12 species of anaerobes, four strains of facultative anaerobes and a strain of pseudomonas aeruginosa, was maintained during the length of the tests (24 or 48 h). the results demonstrated that this device protec ...19751104656
evaluation of a test kit for identification of anaerobic bacteria.a test kit for identification of anaerobic bacteria--api--has been compared for accuracy in individual tests and for identification on the genus or species level with pre-reduced anaerobically sterilized media methods on 241 anaerobic strains. the microsystem was found to be reliable and permits identification of the clinically most significance anaerobic bacteria if it is supplemented with other tests such as gaschromatographic analysis, morphology, lecithinase, lipase and antibiotic sensitivit ...19751105128
[homogenous population of specific igg antibodies in 2 patients with infectious disease characterized by a protracted course]. 19751233778
bacterial arthritis in the adult.a brief review of bacterial arthritis as seen at the mayo clinic in the years 1958 through 1973 has been presented with a selected literature review. once considered a disease of children and adolescents, this disease now is seen more often in adults, especially in those who are elderly, are chronically ill, or are receiving medications that lower the host resistance in invading bacteria. the diagnosis must be made early, with rapid isolation and identification of the organism. proper antibacter ...1975809738
properties of immobilized flavodoxin from peptostreptococcus elsdenii. an affinity ligand for the purification of riboflavin 5'-phosphate (fmn) and its analogues.the small flavoprotein, flavodoxin, isolated from peptostreptococcus elsdenii, has been covalently coupled to cnbr-activated sepharose 4b. the immobilized protein replaces ferredoxin as an electron carrier in hydrogen production from dithionite or pyruvate in the presence of ferredoxin-free extracts of p. elsdenii; compared with soluble flavodoxin, its activities in these systems are 13% and 3.5% respectively. acid treatment reversibly dissociates fmn from the immobilized protein. the dissociati ...19751204624
kinetics and catalytic properties of coenzyme a transferase from peptostreptococcus elsdenii.coenzyme a (coa) transferase from peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. the native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,000). heat treatment of the crude cell extract to 58 degrees c causes proteolysis of the native enzyme and yields a catalytically active enzyme with an approximate molecular weight of ...1976977540
studies on the binding of fmn by apoflavodoxin from peptostreptococcus elsdenii, ph and nacl concentration dependence.1. the ph and ionic strength dependence of the interaction of fmn with apoflavodoxin has been studied by fluorometry in the ph region 2-5, at 22 degrees c. 2. the rate constant of dissociation and the dissociation constant were experimentally determined; the rate constants of association were claculated at a given ph value. these constants depend on the ionic strength. the plots of these constants against the square root of the ionic strength are straight. 3. our data have been interpreted in te ...197610978
fecal steroids and bacterial flora in patients with polyposis coli.fecal neutral and acid steroids and bacterial flora have been studied in a series of patients with polyposis coli and normal controls. the patients with polyposis coli showed a higher concentration of cholesterol and primary bile acids, which increased still more after ileorectostomy. the bacterial flora of the patients with polyposis coli showed an anaerobe/aerobe ratio of 2.7/1 with a relative increase in clostridia and bifidobacteria and decrease in eubacteria and bacteroides. after ileorecto ...19761247152
identification and properties of 8-hydroxyflavin--adenine dinucleotide in electron-transferring flavoprotein from peptostreptococcus elsdenii.1. a new flavin prosthetic group has been isolated in pure form from the electron-transferring flavoprotein of peptostreptococcus elsdenni. its structure has been established as the fad derivative of 7-methyl-8-hydroxyisoalloxazine: (see article). proof of this structure has been obtained by chemical syntehsis of 7-methyl-8-hydroxyisoalloxazine models, and by stepwise degradation of the native compound to 7-methy-8-hydroxyalloxazine. the orange chromophore is characterized by a strong absorption ...19764321
the elimination of urease activity in streptococcus faecium as evidence for plasmid-coded urease.a strain of streptococcus faecium from the sheep rumen showed spontaneous loss of urease activity when subcultured at the normal rumen temperature of 38 degrees c, although in mixed cultures in vivo or in vitro loss of urease was not apparent. the rate of loss of urease in pure cultures was increased at incubation temperatures above 38 degrees c, but loss was never complete. however, at temperatures below 38 degrees c loss was greater, and at 22 or 18 degrees c the urease was completely eliminat ...19761107485
evaluation of amniotic fluid for aerobic and anaerobic bacteria.studies of 117 pregnant women, 83 at term, were instituted to determine the bacteriologic state of amniotic fluid, utilizing both standard aerobic and anaerobic technics. a high association of postpartum infection was found in women who had long periods of premature reptured membranes and many vaginal examinations. significant organisms including anaerobes, were isolated in many of these instances. based on the findings of these studies, it is recommended that amniocentesis for aerobic and anaer ...19761108641
the predominant cultivable organisms in juvenile periodontitis.quantitative and qualitative examinations of the predominant cultivable microflora of the advanced tissue destruction (pocket) associated with juvenile periodontitis and of control pockets without loss of attachment were carried out by using anaerobic tube culture technique. the microflora from the control sites consisted primarily of streptococci, gram-negative rods, and gram-positive rods. in contrast, the test pockets had a relatively stable and characteristic microflora, dominated by various ...19761061986
bacteriological findings in sinusitis (1963-1975).two different studies were performed in order to determine the role of aerobic and anaerobic bacteria in sinusitis. aerobes: a retrospective study of 331 cases of all types of sinusitis (from 1963 till 1975) showed an increasing occurrence of haemophilus influenzae during the last three years. only in the acute sinusitis is streptococcus pneumoniae more frequently found than haemophilus influenzae, which exhibits the highest occurrence in chronic and undeterminable cases. we found cultures with ...19761070150
reconstruction of superior vena cava-right atrial continuity and "anatomic" repair of transposition of great arteries with ventricular septal defect.a superior vena cava-to-right pulmonary artery anastomosis (glenn procedure), used to palliate certain types of cyanotic congenital heart defects, has usually been left intact at the time of definitive intracardiac repair. late failure of this shunt has prompted attempts to reestablish superior vena cava-to-right atrium and right ventricle-to-right pulmonary artery continuity. the present patient, a 10-year-old boy with transposition of the great arteries, ventricular septal defect, and mild pul ...1976768645
medium-dependent inhibition of peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media.of 13 species of anaerobic cocci, peptostreptococcus anaerobius was the only species tested that was sensitive to 0.1% sodium polyanetholsulfonate (sps). however, the sensitivity of p. anaerobius to sps varied according to the media in which the cultures were grown. in supplemented peptone (b-d) and brain heart infusion media, most strain of p. anaerobius were not inhibited by sps. gelatin and proteose peptone were the medium components which were protective. the minimal inhibitory concentration ...1976770497
a bacteriological study of the intestinal mucosa and luminal fluid of adults with acute diarrhoea.bacteriological studies of jejunal mucosal biopsy specimens and contents were performed on 22 hospitalized adult patients with acute diarrhoea and 24 control normal subjects. none of the washed homogenates of the mucosal specimens were sterile and only one fluid specimen obtained from a control subject was sterile. a definite enteric pathogen was found in only five of the 22 diarrhoea patients. there was no qualitative difference in the bacterial profile of the jejunal mucosa and contents of the ...1976779681
human fecal flora: variation in bacterial composition within individuals and a possible effect of emotional are presented on the distribution of 101 bacterial species and subspecies among 1,442 isolates from 25 fecal specimens from three men on: (i) their normal diet and normal living conditions, (ii) normal living conditions but eating the controlled metabolic diet designed for use in the skylab simulation and missions, and (iii) the skylab diet in simulated skylab (isolation) conditions. these bacteria represent the most numerous kinds in the fecal flora. analyses of the kinds of bacteria from ...1976938032
anaerobic bacteria from the large intestine of mice.anaerobic bacteria from the colon of laboratory mice were enumerated and isolated using strict anaerobic techniques. direct microscopic counts revealed 4.4 x 10(10) organisms in each gram (wet weight) of colon contents. actual cultural counts averaged 3.2 x 10(10) organisms, which was 73% of the direct microscopic count. the tentatively identified genera were bacteroides, eubacterium, fusobacterium, lactobacillus, peptostreptococcus, and propionibacterium. strains of fusobacterium, lactobacillus ...1976938042
in vitro susceptibilities of anaerobic bacteria to josamycin.a total of 132 strains of anaerobic bacteria were tested for susceptibility to josamycin, using a broth dilution technique. all strains of peptococcus species, peptostreptococcus species, and bacteroides fragilis were inhibited by 2 mug or less per ml. seventy percent of these susceptible strains were also killed by 2 mug or less of josamycin per ml. however, 2 of 12 clostridium species and 6 of 10 fusobacterium species had minimum inhibitory concentrations of 32 mug or more per ml.1976949184
pulmonary botryomycoma.a patient with a mobile mass of anaerobic organisms in the pulmonary parenchyma is presented. radiographically it resembled a fungous ball. this observed botryomycoma may be part of the spectrum of aspiration pneumonia.1976954467
anaerobic infections in hospital practice.the role of anaerobes in the pathogenesis of infections was investigated. anaerobes were isolated from 0,25% of blood cultures and from 15,8% of other specimens tested; in 15,1% of cases where anaerobes were isolated, no aerobes were found. the strains most commonly encountered were bacteroides fragilis, b. melaninogenicus, peptostreptococcus anaerobius and clostridium perfringens. sensitivity tests in vitro showed all organisms to be sensitive to clindamycin, metronidazole and chloramphenicol, ...1976973160
[brain abscess secondary to pulmonary arteriovenous malformation, case report (author's transl)].a case of brain abscess in the right parietal lobe secondary to pulmonary arteriovenous malformation of the right lung is presented. a 27-year-old man was admitted for headache and left homonymous hemianopsia. cag, vag and brain scan detected brain abscess of the right parietal lobe which was successfully removed surgically. he had been noted to have generalized cyanosis and club finger which corresponded to polycytemia and hypercapnia on examination. subsequently pulmonary arteriovenous malform ...1976988496
Displaying items 101 - 200 of 2255