PMID(sorted ascending)
beta-lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis.this study assessed the extent of beta-lactamase-producing bacteria in subgingival plaque samples obtained from 25 patients with refractory marginal periodontitis in the usa. beta-lactamase-positive isolates were characterized using commercial diagnostic kits and partial sequencing of the 16s rrna gene. the susceptibilities to different antimicrobial agents were tested and, in addition, the isolates were screened for the presence of extended spectrum beta-lactamases (esbls). beta-lactamase-produ ...200415327642
microbiological profile of early onset/aggressive periodontitis patients.the objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence.200415327644
dna-dna relatedness and phylogenetic positions of slackia exigua, slackia heliotrinireducens, eggerthella lenta, and other related bacteria.recently, two asaccharolytic eubacterium species, eubacterium exiguum and eubacterium lentum, and peptostreptococcus heliotrinreducens have been reclassified as slackia exigua, eggerthella lenta and slackia heliotrinireducens in the novel genera on the basis of 16s rdna sequence analysis. but dna-dna relatedness among these species and other related bacteria have not been reported yet. dna-dna relatedness is the standard arbiter and the recommended method for the designation and evaluation of ne ...200415327650
relationship of subgingival and salivary microbiota to gingival overgrowth in heart transplant patients following cyclosporin a therapy.severe gingival overgrowth (go) is induced in patients taking cyclosporin a (csa) following organ transplantation. determining which patient will develop go is still not possible. the purpose of this study was to establish an association between csa and gingival overgrowth in heart transplant patients taking into account periodontal and microbiological conditions.200415341348
intra-abdominal, retroperitoneal, and visceral abscesses in children.this review describes the microbiology, diagnosis, and management of intra-abdominal abscesses (including subphrenic, hepatic, splenic, and retroperitoneal abscesses) in children. they often occur as a complication of local or generalized peritonitis, commonly secondary to appendicitis, necrotizing enterocolitis, pelvic inflammatory disease, and tubo-ovarian infection, surgery or trauma. the original infection generally occurs because of the entry of enteric microorganisms into the peritoneal ca ...200415343468
[biological properties of microorganisms as the basis for the prognosis of the severity of pyoinflammatory pleuropulmonary diseases].in the study of 65 microbial cultures isolated from the purulent foci in acute pulmonary abscess and acute pyothorax of 48 patients, a wide spectrum of microflora was detected. staphylococci and pseudomonas prevailed among aerobes, bacteroids and peptostreptococci--among anaerobes. in cases of the prolonged course of the pathological process, as compared with the common one, microorganisms exhibited hemolytic activity and high antilysozyme and anticomplementary levels. these findings served as t ...200415346941
surgical infections: a microbiological study.surgical infections are mostly polymicrobial, involving both aerobes and anaerobes. one hundred seventeen cases comprised of abscesses (n=51), secondary peritonitis (n=25), necrotizing fascitis (n=22) and wounds with devitalized tissues (n=19) were studied. the number of microorganisms isolated per lesion was highest in secondary peritonitis (2.32). the aerobe/ anaerobe ratio was 0.81 in secondary peritonitis and 1.8 in necrotizing fascitis. most secondary peritonitis (80%), necrotizing fascitis ...200415361989
clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis.the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy.200415367191
small intestinal submucosa tension-free sling: postoperative inflammatory reactions and additional data. 200415371839
variation in antimicrobial action of proanthocyanidins from dorycnium rectum against rumen bacteria.the proanthocyanidin polymer fractions of the leaves of the forage legume dorycnium rectum were analysed by acid catalysis with benzyl mercaptan, nmr and es-ms. the results showed that d. rectum differs from other temperate proanthocyanidin-containing forage legumes in that the range of polymers extends up to very high degrees of polymerisation. three fractions were characterised as low, medium, and high molecular weight proanthocyanidin fractions with mean degree of polymerisations of 10.3, 41 ...200415381413
the role of sequence and structure in protein folding kinetics; the diffusion-collision model applied to proteins l and g.the diffusion-collision model (dcm) is applied to the folding kinetics of protein l and protein g. in the dcm, the two proteins are treated as consisting of two beta-hairpins and one alpha-helix, so that they are isomorphous with the three-helix bundle dcm model. in the absence of sequence dependent factors, both proteins would fold in the same way in the dcm, with the coalescence of the n-terminal hairpin and the helix slightly favored over the c-terminal hairpin and the helix because the forme ...200415458632
the effect of periodontal treatment on the salivary bacterial load and early plaque formation.the purpose of the present study was to investigate in periodontitis patients the relationship between the number of bacteria in the saliva and the amount of de novo plaque formation before and after treatment.200415491312
subgingival microbiota of chronic periodontitis subjects from different geographic locations.most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. the purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries.200415491316
retrospective case review of capsular contracture after two-stage breast reconstruction: is colonization of the tissue expander pocket associated with subsequent implant capsular contracture?periprosthetic capsular contracture is a common problem associated with implant-based breast reconstruction. the purpose of this study was to determine if bacterial colonization of the tissue expander contributes to contracture of the permanent implant. medical records were reviewed for 86 patients (124 tissue expanders) between 1997 and 2001 in 1 institution. three specimens taken from the expander were cultured. the overall incidence of colonization was 42.7%; 49.4% (38.8-60.0) of immediate an ...200415502455
rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis.although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ...200415508748
role of anaerobic bacteria in infections following tracheostomy, intubation, or the use of ventilatory tubes in children.colonization of the tracheobronchial tree with microorganisms almost always follows tracheal intubation, tracheostomy, or the use of ventilatory tubes. infection of the tracheostomy wound site frequently occurs after prolonged use of the tracheostomy. the long-term-ventilated child is at high risk for developing tracheobronchitis or nosocomial pneumonia, generally involving aerobic gram-negative or gram-positive bacteria. several studies have illustrated the role of anaerobic bacteria in lower r ...200415535147
pharmacokinetics and pharmacodynamics of intravenous levofloxacin at 750 milligrams and various doses of metronidazole in healthy adult subjects.the purpose of this investigation was to evaluate the steady-state pharmacokinetics, pharmacodynamics, and safety of intravenous levofloxacin at 750 mg administered once daily combined with three different dosages of intravenous metronidazole (500 mg every 8 h [q8h], 1,000 mg q24h, and 1,500 mg q24h). eighteen healthy adult subjects received all three combinations in a randomized, crossover fashion. serial blood and urine samples were collected on the third day of each study period. the 24-h are ...200415561831
microbiology and management of peritonsillar, retropharyngeal, and parapharyngeal abscesses.this review describes the microbiology, diagnosis, and management of peritonsillar, retropharyngeal, and parapharyngeal abscesses in children. predominant anaerobic organisms isolated in peritonsillar, lateral pharyngeal, and retropharyngeal abscesses are prevotella, porphyromonas, fusobacterium and peptostreptococcus spp.; aerobic organisms are group a streptococcus ( streptococcus pyogenes ), staphylococcus aureus and haemophilus influenzae . anaerobic bacteria can be isolated from most absces ...200415573356
[a case of retroperitoneal abscess by anaerobic bacteria].a case of retroperitoneal abscess with an unknown origin is presented. a 60-year-old woman visited our hospital complaining of fever and right flank pain. abdominal computed tomography and ultrasound examination revealed retroperitoneal abscess posterior to right kidney. percutaneous drainage and administration of antibiotics were performed. then, both symptoms were improved. the fluid culture proved to be anaerobic bacteria, peptostreptococcus species and porphyromonas asaccharolytica. she had ...200415575225
microbiological basis of oral infections and sensitivity to antibiotics.because oral infections are common, the physician must understand the underlying etiology, pathogeny, and other variables that determine how these processes evolve in order to choose the most appropriate antibiotic drug. the special characteristics of the oral cavity determine the make-up of the microflora that lives there. different anaerobic species belonging to the peptostreptococcus, prevotella, fusobacterium, gemella, and porphyromonas genera are of particular interest, as are the aerobic s ...200415580129
periimplantitis.orodental rehabilitation through the use of implants offers very high success rates. in this paper, we describe some of the complications involved with this technique, such as periimplant disease and, within this category, periimplantitis, an inflammatory reaction in which there is a loss of the bony support of the implant accompanied by inflammation. the aetiology of the disease is conditioned by the status of the tissue surrounding the implant, implant design, degree of roughness, the poor ali ...200415580138
socket infection due to retained gauze after evisceration.a 64-year-old man had late-onset socket infection due to retained surgical gauze after evisceration. external examination showed a mass of retained surgical gauze with copious yellowish discharge and gas bubbles. computed tomography scans showed a 3.2 x 2.4 x 2.4-cm heterogeneous mass and numerous gas bubbles. culture of the discharge yielded pseudomonas aeruginosa, streptococcus viridans, peptostreptococcus species, and fusobacterium species. surgical debridement and antibiotic therapy achieved ...200415580975
clinical and microbiological associations in chronic periodontitis.chronic periodontitis is the most prevalent form of periodontal disease. the subgingival microflora has been associated with the onset and progression of the disease. the aim of this work was to establish associations and correlations between clinical indicators and subgingival plaque microbiological data in patients diagnosed with moderate or severe periodontitis. one hundred sites with a probing depth of > or = 5 mm were selected. plaque index (pi), gingival index (gi), bleeding on probing (bp ...200415584257
coaggregation profiles of the microflora from root surface caries lesions.bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. as no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single candida spp. the quantitative coa ...200515598414
anaerobic pulmonary infections in children.pulmonary infections due to anaerobic bacteria usually occur in children prone to aspiration. the source of the anaerobic bacteria is the oropharyngeal bacterial flora, where these organisms outnumber aerobic and facultative organisms in a 10:1 ratio. the most common lower respiratory tract infections where anaerobic bacteria are recovered mixed with aerobic organisms are aspiration pneumonia, lung abscess, and empyema. the predominant isolated anaerobic bacteria are peptostreptococcus, fusobact ...200415599270
[treatment of acute pyoinflammatory diseases of the lungs and pleura under control of microorganism persistence factors].results of treatment of patients with acute pulmonary abscess, acute empyema of the pleura and pyopneumothorax were analyzed. the local treatment of the main group of patients consisting of 50 persons included oxitocin in combination with antiseptics and antibiotics which facilitated maximal reduction of the persistent potential of the pathogenic agent, its rapid elimination from the purulent focus and allowed to get better results using conservative therapy. the obtained clinical effect confirm ...200415626066
[clinical and bacteriological substantiation of the use of cefoperazone/sulbactam in complex therapy of patients with pyo-destructive forms of lower respiratory tract infection (lrti)].antibacterial therapy is the most important component of the complex management of pyo-destructive forms of lrti. since the microbial flora is rather variable and polymorphous, antibiotics used in the treatment of lrti should be active against both aerobic (especially gramnegative) and anaerobic pathogens. the aim of the study was to prove, on the basis of the bacteriological and clinical findings, the validity of the use of cefoperazone/sulbactam (cs), the only inhibitor-protected cephalosporin ...200415628799
estimates of methyl 13c and 1h csa values (deltasigma) in proteins from cross-correlated spin relaxation.simple pulse schemes are presented for the measurement of methyl (13)c and (1)h csa values from (1)h-(13)c dipole/(13)c csa and (1)h-(13)c dipole/(1)h csa cross-correlated relaxation. the methodology is applied to protein l and malate synthase g. average (13)c csa values are considerably smaller for ile than leu/val (17 vs 25 ppm) and are in good agreement with previous solid state nmr studies of powders of amino acids and dipeptides and in reasonable agreement with quantum-chemical dft calculat ...200415630560
b cell superantigens: a microbe's answer to innate-like b cells and natural antibodies.marginal zone b cells and b-1 cells have been termed innate-like b cells as they express limited repertoires that play special roles in immune defenses against common infections. these b cells are the sources of natural antibodies and are capable of highly accelerated clonal responses that help counter blood-borne infections. we have characterized a class of microbial product with highly adapted binding interactions with host immunoglobulins/b cell receptors (bcrs), which enable the targeting of ...200515633015
use of bioinformatics and pcr in the search for abc transporter homology among various bacteria.bioinformatics databases and search tools are utilised to produce polymerase chain reaction (pcr) primers for the amplification of an abc transporter gene from the clinically important anaerobe finegoldia magna. on sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of enterococcus faecalis. little sequence data is available for f. magna and the newly isolated dna could be a useful tool in the identification of this organism in clinical specimens.200415649009
alteration in the endogenous intestinal flora of swiss webster mice by experimental angiostrongylus costaricensis infection.the association between worm infections and bacterial diseases has only recently been emphasized. this study examined the effect of experimental angiostrongylus costaricensis infection on endogenous intestinal flora of swiss webster mice. eight mice aging six weeks were selected for this experiment. four were infected with a. costaricensis and the other four were used as controls. twenty eight days after the worm infection, all mice in both groups were sacrificed and samples of the contents of t ...200415654427
synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein l from peptostreptococcus magnus.rational design and combinatorial chemistry were utilized to search for lead protein l (ppl) mimetics for application as affinity ligands for the purification of antibodies and small fragments, such as fab and scfv, and as potential diagnostic or therapeutic agents. inspection of the key structural features of the complex between ppl and human fab prompted the de novo design and combinatorial synthesis of a 169-membered solid-phase ligand library, which was assessed for binding to human igg and ...200515688433
periodontal infections and pre-term low birth weight: a case-control study.pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ...200515691348
traumatic cholesteatoma presenting as a brain abscess: a case discuss the clinical aspects and management of traumatic cholesteatomas of the temporal bone.200515699721
recovery of anaerobic bacteria from wounds after lawn-mower injuries.accidental injury while using lawn mowers can cause serious infectious complications in the injured extremity. anaerobic bacteria were rarely recovered from this infection. two children who sustained injury in their foot by a lawn mower developed severe wound infection. culture of the wound from 1 patient had heavy growth of clostridium bifermentans and peptostreptococcus magnus, and the culture from the other child grew clostridium perfringens. antimicrobial therapy directed at the pathogens an ...200515699821
pharmacokinetics and pharmacodynamics of linezolid in obese patients with cellulitis.linezolid is an oxazolidinone antimicrobial with excellent oral bioavailability and tissue penetration and is active against multidrug-resistant skin/soft tissue pathogens.200515701775
influence of antimicrobial treatment for helicobacter pylori infection on the intestinal microflora in japanese macaques.eradication treatment for helicobacter pylori is known to cause mild but relatively frequent adverse effects. some adverse effects such as diarrhoea and soft stools are related to disruption of the composition of the intestinal microflora. this study investigated the microfloral changes resulting from administration of an eradication regimen using proton pump inhibitor (ppi), amoxycillin and clarithromycin. twenty-eight laboratory-bred japanese macaques either were administered eradication treat ...200515713617
micromonas (peptostreptococcus) micros: unusual case of prosthetic joint infection associated with dental procedures.micromonas (peptostreptococcus) micros is frequently associated with periodontal disease as well as respiratory, gastrointestinal and female genitourinary tract infections, but only rarely has been reported as a pathogenic agent of prosthetic joint infections. here we describe a case of a 63-year-old woman with prosthetic joint infection of total hip arthroplasty caused by the anaerobic species micromonas micros, associated with tooth extraction. samples obtained intraoperatively and from the or ...200515715175
binding of actinobacillus actinomycetemcomitans lipopolysaccharides to peptostreptococcus micros stimulates tumor necrosis factor alpha production by macrophage-like cells.peptostreptococcus micros is a gram-positive bacterium that has been associated with periodontitis and endodontic infections. in this study, we hypothesized that p. micros binds the immunomodulating component lipopolysaccharide derived from gram-negative bacteria to increase its capacity to stimulate cytokine production by host cells. the ability of p. micros to bind actinobacillus actinomycetemcomitans lipopolysaccharide was demonstrated by an enzyme-linked immunosorbent assay and by immunoelec ...200515720573
properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an escherichia coli host.peptostreptococcus asaccharolyticus glutamate dehydrogenase (l-glutamate: nad+ oxidoreductase (deaminating); ec overexpressed in escherichia coli has been purified by two new methods. enzyme made by the first method showed remarkable thermophilicity, with a temperature optimum of 60 degrees c, and also thermostability, which suggested the second, simpler method, incorporating a heat step. this produced 94 mg of homogeneous protein per litre culture medium. the basic kinetic parameters f ...200515727821
[frequency, diagnostic criteria and consequences of bacterial vaginosis in pregnant women].vaginal discharge based on changes in a vaginal ecosystem (decreasing number of lactobacillus and increasing number of mobiluncus spp., gardnerella vaginalis, prevotella spp., mycoplasma hominis, peptostreptococcus spp) appears to be a major cause of bacterial vaginosis. bacterial vaginosis is associated with: preterm delivery, premature rupture of membranes, chorioamnionitis, amniotic fluid infection, endometritis, low-weight neonates. the role of natural microflora of vagina, the most importan ...200415730019
engineering high affinity superantigens by phage display.protein l (ppl) is a b-cell superantigen from peptostreptococcus magnus known to bind to mammalian vkappa light chains. ppl from p.magnus strain 312 comprises five homologous immunoglobulin (ig) binding domains. we first analysed the binding of the individual domains (b1-b5) of ppl(312) to human vkappa light chains (huvkappa) subtypes 1 (huvkappai) and 3 (huvkappaiii). using a combination of rational design and phage selection we isolated mutants of the n-terminal b1 domain with a 14-fold increa ...200515733921
microbiology and management of infected neck review and describe the microbiology, diagnosis, and management of infected neck cysts.200515742293
[pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology].this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria.200515757581
anaerobic spondylodiscitis: case series and systematic review.bacterial spondylodiscitis is rarely caused by anaerobic organisms. we describe two patients with lumbar vertebral osteomyelitis and discitis caused by anaerobic bacteria, including an unusual occurrence after an endodontic procedure, and review the salient clinical features and outcomes of 31 previously reported cases.200515759942
influence of denatured and intermediate states of folding on protein aggregation.we simulate the aggregation thermodynamics and kinetics of proteins l and g, each of which self-assembles to the same alpha/beta [corrected] topology through distinct folding mechanisms. we find that the aggregation kinetics of both proteins at an experimentally relevant concentration exhibit both fast and slow aggregation pathways, although a greater proportion of protein g aggregation events are slow relative to those of found for protein l. these kinetic differences are correlated with the am ...200515772307
protein l-agarose for adsorption of autoantibodies: a potential tool for extracorporeal treatment.this work investigated the adsorption of autoantibodies such as anti-ss-a/ro, anti-ss-b/la, anti-sm, and anti-dsdna on protein l-agarose gel. in order to determine better conditions for igg adsorption on this matrix, some buffer systems were tested. adsorption data were analyzed using the langmuir and langmuir-freundlich isotherm models. the experimental isotherms were best described by the langmuir-freundlich model, which indicated negative and positive cooperativities for binding in the presen ...200515787626
detection of bacteria in platelet concentrates: comparison of broad-range real-time 16s rdna polymerase chain reaction and automated culturing.based on real-time polymerase chain reaction (pcr) technology, a broad-range 16s rdna assay was validated and its performance was compared to that of an automated culture system to determine its usefulness for rapid routine screening of platelet concentrates (pcs).200515847662
chicken anti-protein l for the detection of small amounts of protein l in the presence of igg.protein l is a cell surface protein, expressed by peptostreptoccocus magnus, which binds to the variable light chains of immunoglobulins without interfering with antigen binding. it can be used for purification of mammalian antibodies of all classes in contrast to the ig-binding proteins protein a and protein g. detection of protein l leakage into antibody preparations is important, since protein l could interfere in immunological assays and cause adverse reactions in vivo. here we have develope ...200515857176
mechanically unfolding the small, topologically simple protein l.beta-sheet proteins are generally more able to resist mechanical deformation than alpha-helical proteins. experiments measuring the mechanical resistance of beta-sheet proteins extended by their termini led to the hypothesis that parallel, directly hydrogen-bonded terminal beta-strands provide the greatest mechanical strength. here we test this hypothesis by measuring the mechanical properties of protein l, a domain with a topology predicted to be mechanically strong, but with no known mechanica ...200515863479
microbiology of acute and chronic maxillary sinusitis associated with an odontogenic origin.: to study the microbiology of sinusitis associated with odontogenic origin.200515867647
tobacco smoking and periodontal microflora in a saudi arabian explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits.200515882210
bacteriology of chronic maxillary sinusitis associated with nasal polyposis.aspirates from 48 chronically inflamed maxillary sinuses from patients who had nasal polyposis were processed for aerobic and anaerobic bacteria. bacterial growth was present in 46 (96%) specimens. aerobic or facultative bacteria were present in 6 (13%) specimens, anaerobic bacteria alone in 18 (39%), and mixed aerobic and anaerobic bacteria in 22 (48%). there were 110 bacterial isolates (2.4 per specimen). thirty-nine of the isolates were aerobic or facultative organisms (0.85 per specimen). th ...200515888469
associations between igg antibody to oral organisms and carotid intima-medial thickness in community-dwelling adults.the aims of this study are to describe the relationships between igg antibodies to 17 oral organisms and atherosclerosis as indexed by carotid intima-medial wall thickness (imt) and to evaluate the role of smoking.200515893320
infected neonatal cephalohematomas caused by anaerobic present the microbiological and clinical features of six children with infected cephalohematomas (ic) caused by anaerobic bacteria.200515914351
identification of cultivable microorganisms from primary endodontic infections with exposed and unexposed pulp space.this study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. the canal contents were analyzed by aerobic/anaerobic culture, and conventional identification techniques. there were 211 isolates of bacteria belonging to 28 genera and 55 species recovered from exposed canals. in the unexpose ...200515917680
comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens.the purpose of this study was to compare the antimicrobial activities of 6% and 3% sodium hypochlorite (naocl), 2% and 0.12% chlorhexidine gluconate (chx), and 0.01% and 0.005% doxycycline (doxy) on four microorganisms associated with primary endodontic infections. the agar diffusion test was used to measure antimicrobial activities of these agents against peptostreptococcus micros, prevotella intermedia, streptococcus sanguis, and lactobacillus acidophilus. minimum inhibitory concentration anal ...200515917691
periodontal pathogens: a quantitative comparison of anaerobic culture and real-time pcr.periodontitis is a multi-factorial chronic inflammatory and destructive disease of the tooth-supporting tissues. quantitative anaerobic culture techniques have been used for microbial diagnosis of the different forms of the disease. the aim of this study was to compare real-time pcr with quantitative anaerobic culture for detection and quantification of 5 prominent periodontal pathogens. real-time pcr assays with the 16s rrna genes of actinobacillus actinomycetemcomitans, prevotella intermedia, ...200515919188
different crystal packing in fab-protein l semi-disordered peptide complex.proteins and peptides with variable degrees of disorder are a challenge for protein crystallization. these may be completely disordered or just contain regions with a high degree of mobility that may be represented by a multitude of discretely defined conformations. these difficulties are not insurmountable, but it may be unreasonable to expect a clean result from a structural point of view. the complex between a murine monoclonal antibody (19d9d6) and a synthetic peptide that encompasses the fi ...200515930632
comparison of the crystallization and crystal packing of two fab single-site mutant protein l complexes.protein l from peptostreptococcus magnus (ppl) is a multidomain protein composed of four or five immunoglobulin-binding domains that target the kappa light chain of a large repertoire of human and murine antibodies. thus, a single domain of this protein can be used to aid the crystallization of fab, free or complexed to their antigen when it is not possible to obtain crystals without it. each wild-type ppl domain has two light-chain binding sites that target the same region of the light chain an ...200515930633
meningioma associated with abscess formation--a case report.a rare case of meningioma associated with both intratumoral and peritumoral abscess formation occurred in a 38-year-old man presenting with signs and symptoms of elevated intracranial pressure, intracranial infection, and right temporal pole mass lesion. the mass lesion was totally removed, revealing a meningioma. group b streptococcus and peptostreptococcus were cultured from both the tumor and peritumoral white matter. hematogenous spread of the organisms related to recent dental work was the ...200515936397
bacteriology of chronic dacryocystitis in a tertiary eye care determine the current bacteriology of adult chronic dacryocystitis in a tertiary eye care center and compare it with previously reported studies.200515942496
antimicrobial susceptibility variation of 50 anaerobic periopathogens in aggressive periodontitis: an interindividual variability study.the frequent use of antibiotics in developed countries has led to the emergence of widespread bacterial resistance. in this study, the interindividual variability of the antibiotic susceptibility of 50 putative microorganisms in aggressive periodontitis patients has been evaluated by means of vc (variation coefficient).200515943770
recovery of potential pathogens and interfering bacteria in the nasopharynx of smokers and smoking and passive exposure to cigarette smoke are associated with colonization by some potentially pathogenic species of bacteria and an increased risk of respiratory tract infection in both adults and children. in an attempt to explain these observations, this study compared the frequency of isolation of potential pathogens, and aerobic and anaerobic bacteria that possess interfering capabilities (ie, interfering with the in vitro growth of potential pathogens) in the nasopharynx of sm ...200515947322
prevalence of periodontopathic bacteria in aggressive periodontitis patients in a chilean population.actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis (agp). other periodontopathic bacteria such as porphyromonas gingivalis are also suspected of participating in aggressive periodontitis although the evidence to support this is controversial. the aim of the present study was to determine the prevalence of eight periodontopathic bacteria in chilean patients with agp.200515974855
large thigh abscess after placement of synthetic transobturator report a unique complication associated with transobturator slings.200615986099
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004].tendency of isolated bacteria from infections in general surgery during the period from april 2003 to march 2004 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 455 strains including 14 strains of candida spp. were isolated from 191(75.2%) of 254 patients with surgical infections. two hundred and thirty-nine strains were isolated from primary infections, and 216 strains were isolated from postoperative infections. from primary infection ...200515997655
on the origin of the thermostabilization of proteins induced by sodium phosphate.the b1 domain of protein l shows a linear rise in thermostability with increasing concentrations of sodium phosphate. equal behavior is observed for a set of mutant proteins where surface lysines are mutated to noncharged residues, but the mutant's thermostabilities show different sensitivities to phosphate, encoded in the varying slopes observed (mi). the melting temperature in the absence of the cosolute also correlates linearly with mi. the stabilizing effect of the phosphate ion reaches a sa ...200515998065
antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in the netherlands and spain.antimicrobial resistance of periodontal pathogens towards currently used antibiotics in periodontics has been investigated in a previous study. microbial resistance in the periodontal microflora was more frequently observed in spanish patients in comparison with dutch patients. the aim of the present study was to compare antimicrobial susceptibility profiles of five periodontal bacteria isolated from periodontitis patients in spain and in the netherlands.200515998275
bacteriology of acute and chronic ethmoid sinusitis.aspirates from 26 acutely and 17 chronically infected ethmoid sinuses were studied. thirty-seven aerobes and 10 anaerobes were recovered from isolates from patients with acute sinusitis. streptococcus pneumoniae and haemophilus influenzae were predominant. twenty-seven aerobes and 41 anaerobes were found in isolates from patients with chronic sinusitis. the predominant isolates were anaerobic gram-negative bacilli and peptostreptococcus spp.200516000483
toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria.the colonic epithelium provides an interface between the host and micro-organisms colonising the gastrointestinal tract. molecular recognition of bacteria is facilitated through toll-like receptors (tlr). the colonic epithelium expresses relatively high levels of mrna for tlr3 and less for tlr2 and -4. little is known of the expression patterns and mode of induction of expression for these pattern recognition receptors in human colon. the aim of this study was to investigate their localization i ...200516011525
bacterial diversity in necrotizing ulcerative periodontitis in hiv-positive subjects.necrotizing ulcerative periodontitis (nup) is a painful and potentially debilitating affliction that affects about 2% to 6% of hiv-positive subjects. nup may be caused by specific microorganisms that are presently unknown or by microbial species not usually thought to cause periodontal infections. the purpose of this study was to define the bacterial species associated with nup in hiv-positive patients.200216013212
the role of bacterial interference in otitis, sinusitis and tonsillitis.bacterial interactions that include antagonism (interference) and synergism help maintain balance between the members of the normal endogenous flora. alpha-streptococci that predominate in the normal respiratory tract flora attracted most attention in studies of bacterial interference. other organisms that possess interfering characteristics in upper respiratory tract infections (urtis) are nonhemolytic streptococci, and prevotella and peptostreptococcus spp. the production of bacteriocins by so ...200516025067
[comparison of the detection of anaerobic bacteria in haemoculture systems bactec lytic/10 anaerobic/f, bact/alert fan anaerobic and bact/alert fn anaerobic].the purpose of this study is to deal with the problem of anaerobic cultivation of clinical specimens and consider the possibility of using semi-automated blood culture instruments. the bactec lytic, bact/alert fan anaerobic and bact/alert fn anaerobic bottles were inoculated with bacteriodes fragilis, clostridium perfringens, peptostreptococcus anaerobius and feingoldia magna strains. the times to detection (ttd) for positive bottles were evaluated with reference to the number of inoculated bact ...200516025428
genotypic characterization of bacteria cultured from duck characterize the bacterial composition of mallard duck faeces and determine if novel bacterial species are present that could be utilized as potential indicators of avian faecal contamination.200516033461
trypanosoma cruzi: influence of predominant bacteria from indigenous digestive microbiota on experimental infection in verify the influence of some predominant components from indigenous microbiota on systemic immunological responses during experimental chagas disease, germ-free nih swiss mice were mono-associated with escherichia coli, enterococcus faecalis, bacteroides vulgatus or peptostreptococcus sp. and then infected with the y strain of trypanosoma cruzi. all the mono-associations predominantly induced a th1 type of specific immune response to the infection by t. cruzi. a direct correlation was observe ...200516038901
cervical necrotizing fasciitis: an unusual complication of genuine peritonsillar abscess. 200516048699
identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis.most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ...200516081935
[efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study].erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens.200516087299
antibacterial activity of aqueous extracts of selected chewing sticks.this aim of this study was to determine the antibacterial activity in extracts obtained from various nigerian chewing sticks. aqueous extracts from seventeen chewing sticks and the fruit of c. ferruginea, one fruit used in oral hygiene in nigeria, were screened for antibacterial activity against type cultures of staphylococcus aureus, bacillus subtilis, escherichia coli, and pseudomonas aeruginosa. eleven of the test extracts showed activity against at least two of these referenced organisms. mi ...200516127476
microbiology of acute exacerbation of chronic sinusitis.we undertook to evaluate the microbiology of acute exacerbation of chronic sinusitis (aecs).200516134356
[role of obligate anaerobes in infections in hospitalized patients and therapeutic options].monitoring of prevalence and antibiotic susceptibility of strictly anaerobic bacteria, causing infections in hospitalized patients, constitutes a part of a program for prudent use of antibiotics. the aim of the study was to assess prevalence of strictly anaerobic bacteria in patients hospitalized in a tertiary care hospital in 2001-2002 with reference to empiric antibiotic therapy. the most common gram-positive bacteria were clostridium difficile--27.7%, peptostreptococcus spp. and peptoniphilus ...200516134392
[the occurrence of microorganisms in intraoral abscesses].the aim of this study was to assess the occurrence of microorganisms in 39 intraoral abscesses. the samples were place in transport medium. the materials were inoculated on adequate enrichment and selective media and cultivated under anaerobic and aerobic conditions. the microorganisms were found in all samples (100%), anaerobic bacteria most frequently were recovered. the predominant anaerobes were from genus prevotella, bacteroides, fusobacterium and peptostreptococcus. among aerobic bacteria, ...200516134393
[surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2003].for the post-marketing surveillance of cefmetazole (cmz, cefmetazon), mics of injectable beta-lactam antibacterials including cmz against clinical isolates from 15 medical institutions all over japan are measured yearly and the incidence rates of resistance in various species are also evaluated. in the first surveillance from june 2000 to march 2001, 574 isolates of 13 species were tested, 548 isolates of the same 13 species were tested in the second surveillance from april 2001 to march 2002, a ...200516161756
identification and antimicrobial susceptibility of micro-organisms recovered from cutaneous lesions of human american tegumentary leishmaniasis in minas gerais, evaluation of the microbiota present in cutaneous ulcers from 31 patients with a clinical and parasitological diagnosis of american tegumentary leishmaniasis (atl) was carried out by the standard filter paper disc technique, including antimicrobial susceptibility of the bacterial isolates. microbial examination indicated that 21 patients (67.7%) were contaminated with one to four bacteria and some of them also with yeast. a total of 142 micro-organisms were isolated. staphylococcus aureus was ...200516192439
intestinal bacterial communities that produce active estrogen-like compounds enterodiol and enterolactone in humans.lignans are dietary diphenolic compounds which require activation by intestinal bacteria to exert possible beneficial health effects. the intestinal ecosystem plays a crucial role in lignan metabolism, but the organisms involved are poorly described. to characterize the bacterial communities responsible for secoisolariciresinol (seco) activation, i.e., the communities that produce the enterolignans enterodiol (ed) and enterolactone (el), a study with 24 human subjects was undertaken. seco activa ...200516204524
chronic sinusitis in children and adults: role of bacteria and antimicrobial management.the nasopharynx serves as the reservoir for anaerobic bacteria as well as pathogenic bacteria that can cause respiratory infections including sinusitis. some of these organisms possess the ability to interfere with the growth of potential pathogens and may play a role in preventing infections. anaerobic bacteria emerge as pathogens as the infection becomes chronic. this may result from the selective pressure of antimicrobial agents that enable resistant anaerobic organisms to survive, and from t ...200516216174
[soft tissue infections in oral, maxillofacial, and plastic surgery. bacterial spectra and antibiotics].soft tissue infections in the maxillofacial region are mainly caused by staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli but also by members of the genera enterococcus, klebsiella, and enterobacter, respectively.200516228187
antibacterial action of photoactivated disinfection {pad} used on endodontic bacteria in planktonic suspension and in artificial and human root measure antibacterial action of photoactivated disinfection (pad) on endodontic bacteria in planktonic suspension and root canals. methods: four bacteria, fusobacterium nucleatum,peptostreptococcus micros, prevotella intermedia and streptococcus intermedius, were tested in suspension. after mixing equal volumes of tolonium chloride and bacterial suspension for 60s, each 200 microl of concentration (>10(6)cfu ml(-1)) was irradiated with light at 633+/-2 nm. each energy dose/tolonium chloride c ...200616239058
microbial analysis of the autogenous bone collected by bone filter during oral surgery: a clinical study.the purposes of the study presented are to identify the microbial content of the collected bone debris and to determine the antibacterial efficiency of chlorhexidine mouth rinse in reducing the microbial content of the collected bone debris.200516243175
[bacterial spectra and antibiotics in odontogenic infections. renaissance of the penicillins?].the role played by odontogenic infection in dental, oral, and maxillofacial surgery is not to be underestimated even at the present time. an extensive, standardized, prospective study was performed with the intention of verifying the bacterial spectrum of odontogenic infections to evaluate antibiotic sensitivity.200516261393
effect of sodium alendronate on alveolar bone resorption in experimental periodontitis in rats.bisphosphonates are potent inhibitors of bone resorption and were shown to inhibit bone resorption in experimental periodontitis by unknown mechanisms. we studied the effect of the aminobisphosphonate sodium alendronate (sa) in experimental periodontitis. wistar rats were subjected to ligature placement around the second upper left molars.200516274309
[two cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae].most of bartholin's gland abscesses have been thought to be caused by microorganisms found in opportunistic infections. however, we have encountered two very interesting cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae, two major pathogens of respiratory tract infections. in the first case, since abscess formation was not observed due to disintegration, cefdinir (cfdn), 300 mg/day, t.i.d. for 5 days was administered. the treatment improved clinic ...200516276738
the effect of haart on salivary microbiota in the women's interagency hiv study (wihs).study the prevalence of potentially pathogenic microorganisms in saliva of hiv-positive women in the women's interagency hiv study.200516301151
microbiology of intracranial abscesses and their associated evaluate the organisms recovered from infected sinuses and associated intracranial abscesses (ias).200516301376
the in-vitro activity of three long-acting cephalosporins against bacteroides fragilis, peptostreptococcus species and clostridium perfringens. 198916312326
in vitro bactericidal activity of the glycopeptide compounds vancomycin, teicoplanin and ramoplanin (a-16686/mdl 62,198). 198916312373
bacterial etiology of diabetic foot infections in south india.foot infections are a frequent complication of patients with diabetes mellitus, accounting for up to 20% of diabetes-related hospital admissions. infectious agents are associated with the worst outcomes, which may ultimately lead to amputation of the infected foot unless prompt treatment strategies are ensued. the present study sought to reveal the bacterial etiology of diabetic foot ulcers in south india, the diabetic capital of india.200516314237
microbiology profile in women with pelvic inflammatory disease in relation to iud study the microbial characteristics of patients with pelvic inflammatory disease (pid) and the possible impact of an intrauterine device (iud) on the microbial environment in women presenting with pid.200516338777
[bacterial 16s rdna sequence analysis of siberian tiger faecal flora].bacterial 16s rdna library of siberian tiger was developed and 15 different clones were obtained using ecor i and hind iii in restriction fragment length polymorphism analysis. dna sequencing and similarity analysis showed that 10 clones matched corresponding clostridium sequences, of which 6 sequences had over 99% similarity with clostridium novyi type a, and 4 sequences had 97% similarity with swine manure bacterium rt-18b, which identified as peptostreptococcus spp. the other five 16s rdna se ...200516342753
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