PMID(sorted ascending)
mice lacking monocyte chemoattractant protein 1 have enhanced susceptibility to an interstitial polymicrobial infection due to impaired monocyte recruitment.monocyte chemoattractant protein 1 (mcp-1) is an important chemokine that induces monocyte recruitment in a number of different pathologies, including infection. to investigate the role of mcp-1 in protecting a host from a chronic interstitial polymicrobial infection, dental pulps of mcp-1(-/-) mice and controls were inoculated with six different oral pathogens. in this model the recruitment of leukocytes and the impact of a genetic deletion on the susceptibility to infection can be accurately a ...200212011011
aerobic and anaerobic microbiology of suppurative sialadenitis.aspirates of pus from acute suppurative sialadenitis were investigated for the presence of aerobic and anaerobic bacteria. a total of 47 specimens, 32 from parotid, 9 from submandibular and 6 from sublingual glands yielded bacterial growth. fifty five isolates, 25 aerobic and 30 anaerobic, were isolated from parotid infection: anaerobic bacteria only were detected in 13 (41%) specimens, aerobic or facultative bacteria only in 11 (34%) and mixed aerobic and anaerobic bacteria in 8 (25%). of a tot ...200212018662
physical and genetic map of the finegoldia magna (formerly peptostreptococcus magnus) atcc 29328 genome.a physical and genetic map of finegoldia magna (formerly peptostreptococcus magnus) atcc 29328 chromosome was constructed. the order of rare cleavage restriction fragments was determined by double digestion with the restriction enzymes i-ceui, sgrai, apai and pmei, cross-hybridization and apai-linking clones. the size of the circular chromosome of f. magna was estimated to be 1.9 mb. this strain also had a 200-kb megaplasmid. the chromosome contained four rrn operons, and the orientation of two ...200212023074
deuterium spin probes of side-chain dynamics in proteins. 1. measurement of five relaxation rates per deuteron in (13)c-labeled and fractionally (2)h-enriched proteins in pulse sequences are presented for the measurement of the relaxation of deuterium double quantum, quadrupolar order, and transverse antiphase magnetization in (13)ch(2)d methyl groups of (15)n-, (13)c-labeled, fractionally deuterated proteins. together with previously developed experiments for measuring deuterium longitudinal and transverse decay rates [muhandiram, d. r.; yamazaki, t.; sykes, b. d.; kay, l. e. j. am. chem. soc. 1995, 117, 11536], these schemes allow measurement of the five un ...200212033875
deuterium spin probes of side-chain dynamics in proteins. 2. spectral density mapping and identification of nanosecond time-scale side-chain the previous paper in this issue we have demonstrated that it is possible to measure the five different relaxation rates of a deuteron in (13)ch(2)d methyl groups of (13)c-labeled, fractionally deuterated proteins. the extensive set of data acquired in these experiments provides an opportunity to investigate side-chain dynamics in proteins at a level of detail that heretofore was not possible. the data, acquired on the b1 domain of peptostreptococcal protein l, include 16 (9) relaxation measu ...200212033876
reclassification of clostridium hydroxybenzoicum as sedimentibacter hydroxybenzoicus gen. nov., comb. nov., and description of sedimentibacter saalensis sp. nov..strain zf2t, isolated from freshwater sediment, is a motile, rod-shaped, gram-positive, endospore-forming, amino acid- and pyruvate-utilizing, anaerobic bacterium. it requires yeast extract for growth. carbohydrates are not utilized. the optimal temperature and ph for growth are 37 degrees c and 6.8-7.3, respectively. the g+c content of the dna is 34.0 mol %. a 16s rdna sequence analysis of strain zf2t revealed that the highest similarity (94.4%) was shared with clostridium hydroxybenzoicum jw/z ...200212054241
effect of deuteration on some structural parameters of methyl groups in proteins as evaluated by residual dipolar bond methyl 1h-13c and 13cmethyl-13c scalar and residual dipolar couplings have been measured at sites in an 15n, 13c, approximately 50% 2h labeled sample of the b1 immunoglobulin binding domain of peptostreptococcal protein l to investigate changes in the structure of methyl groups in response to deuterium substitution. both one bond methyl 1h-13c and 13cmethyl-13c scalar coupling constants have been found to decrease slightly with increasing deuterium content. previous studies have shown t ...200212061716
[clinical aspects of abscess development in parotitis].the purpose of this paper was to analyse parotideal abscesses on the basis of a larger number of patients.200212063691
[sensitivity of the folding pathway to the details of amino acid sequence].experimental data on the structure of transition state demonstrate that the proteins with the same topology as a rule have similar folding nuclei (the structured formed part of the transition state). in this review we discuss the experimental works which show that the position of folding nuclei is different among proteins with the same topology. these facts underline that the folding pathway is sensitive to the details of amino-acid sequence.200212068621
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--i. gram-positive bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, and penicillins. fifteen species, 1,062 strains, of gram-positive bacteria were isolated from the clinical materials annually collected from january to december, and consisted of methicillin-susceptible staphylococcus aureus (mssa; n = 127), methicillin-resistant s ...200212071093
peptostreptococcal pericarditis complicating anti-tumour necrosis factor alpha treatment in rheumatoid arthritis. 200212079913
invasion of dentinal tubules by oral bacteria.bacterial invasion of dentinal tubules commonly occurs when dentin is exposed following a breach in the integrity of the overlying enamel or cementum. bacterial products diffuse through the dentinal tubule toward the pulp and evoke inflammatory changes in the pulpo-dentin complex. these may eliminate the bacterial insult and block the route of infection. unchecked, invasion results in pulpitis and pulp necrosis, infection of the root canal system, and periapical disease. while several hundred ba ...200212097359
bacterial superinfection in human tungiasis.tungiasis is caused by penetration of the female sand flea tunga penetrans into the epidermis of its host. it is endemic in many countries in latin america, the caribbean and sub-saharan africa. although superinfection is a common clinical observation, the frequency and the pattern of bacterial pathogens associated with tungiasis have never been investigated systematically. we conducted a prospective clinico-bacteriological study with patients living in a shantytown in fortaleza, capital of cear ...200212100437
actinobacillus actinomycetemcomitans proportion of subgingival bacterial flora in relation to its clonal type.we investigated whether certain actinobacillus actinomycetemcomitans clones occur in elevated proportions in subgingival flora, and if the proportions relate to other bacteria in the samples. a total of 121 a. actinomycetemcomitans strains from 121 patients with periodontitis were serotyped and 60 strains were also genotyped. the 121 strains were divided into three groups and the 60 strains into two groups according proportion of a. actinomycetemcomitans. the samples from the 60 patients with ge ...200212120706
cutaneous and subcutaneous infections in newborns due to anaerobic bacteria.this review describes the microbiology and management of the major cutaneous and subcutaneous infections in newborns where anaerobic bacteria predominate: omphalitis, necrotizing fasciitis, breast abscess, and scalp infection following intrauterine fetal monitoring. the predominant bacteria known to cause these infections are group b streptococcus, group d enterococcus, group a streptococcus, staphylococcus aureus, enterobacteriaceae, and anaerobic bacteria. all of these agents can colonize or i ...200212122901
clinical review: bacteremia caused by anaerobic bacteria in children.this review describes the microbiology, diagnosis and management of bacteremia caused by anaerobic bacteria in children. bacteroides fragilis, peptostreptococcus sp., clostridium sp., and fusobacterium sp. were the most common clinically significant anaerobic isolates. the strains of anaerobic organisms found depended, to a large extent, on the portal of entry and the underlying disease. predisposing conditions include: malignant neoplasms, immunodeficiencies, chronic renal insufficiency, decubi ...200212133179
design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ...200212167534
randomized, double-blind, multicenter comparison of oral cefditoren 200 or 400 mg bid with either cefuroxime 250 mg bid or cefadroxil 500 mg bid for the treatment of uncomplicated skin and skin-structure infections.uncomplicated skin and skin-structure infections are commonly observed in medical practice. because these infections typically are confined to the superficial layers and seldom lead to the destruction of skin structures and resultant systemic dissemination, in general they can be treated with an oral antibiotic with potent microbiologic activity against gram-positive pathogens.200212182257
combinations of bacterial species in endodontic infections.this study was undertaken to investigate combinations of bacteria found in root-canal infections of teeth with periapical bone destruction without clinical signs and symptoms.200212196223
genotypic diversity of clinical actinomyces species: phenotype, source, and disease correlation among genospecies.we determined the frequency distribution of actinomyces spp. recovered in a routine clinical laboratory and investigated the clinical significance of accurate identification to the species level. we identified 92 clinical strains of actinomyces, including 13 strains in the related arcanobacterium-actinobaculum taxon, by 16s rrna gene sequence analysis and recorded their biotypes, sources, and disease associations. the clinical isolates clustered into 21 genogroups. twelve genogroups (74 strains) ...200212202591
comparative in vitro activity of faropenem and 11 other antimicrobial agents against 405 aerobic and anaerobic pathogens isolated from skin and soft tissue infections from animal and human bites.faropenem, a new oral beta-lactam agent with a penem structure, was very active against 405 aerobic and anaerobic bite isolates. it inhibited 232 of 236 (98%) aerobic isolates, including all pasteurella spp. and eikenella corrodens at < or = 0.25 mg/l, and 164/169 (97%) anaerobic isolates, at < or = 1 mg/l. the 10 isolates that required > or = 2 mg/l for inhibition were one strain each of acinetobacter lwoffi, corynebacterium minutissimum, bacteroides ovatus, lactobacillus delbrueckii and peptos ...200212205068
[anaerobic bacterial analysis of 436 specimens of clinical infection].this study was aimed at the relationship between anaerobes and clinical infection. aerobic and anaerobic bacterial culture was performed for 436 specimens of clinical infection. the incidence of anaerobic bacteria isolates and predominant species were analyzed. the results showed that the incidence of anaerobic isolates was 88.3% (385/436), the main predeminoue species were gram negative nonsporeforming anaerobic rods (bacteroids, prevotella and porphyromonas) and peptostreptococcus. the endogen ...199912205907
[endogenous intragastrical microflora in gastric cancer]the aim of this prospective study was to identify the type of microbial flora and additionally to correlate the total number of microorganisms with factors such as age, tumoral area, and the ph gastric in unaffected gastric mucosal biopsies obtained during gastrojejunostomy (n=5) or gastrectomy (n=1) in patients with gastric adenocarcinoma (n=15) or lymphoma (n=1) at the belen hospital, trujillo, peru, between january 1, 1996 and april 30,1998. the mean age of the patients was 54.4 + 18.3 years ...199812209218
evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein l interface.the multidomain bacterial surface protein l (ppl) is a virulence factor expressed by only 10% of peptostreptococcus magnus strains, and its expression is correlated with bacterial vaginosis. the molecular basis for its ability to recognize 60% of mammalian immunoglobulin light chain variable regions (v(l)) has been described recently by x-ray crystallography, which suggested the presence of two v(l) binding sites on each protein l domain (graille, m., stura, e. a., housden, n. g., beckingham, j. ...200212221088
[a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers].a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ...198512339972
protein l mutants for the crystallization of antibody many cases, antibody and their complexes can be crystallized and their structure determined without major difficulties. the remaining problematic cases may be approached through techniques such as of combinatorial complex crystallization which uses immunoglobulin binding proteins (ibp). the range of lattices that can be made using this method can be expanded by engineering mutants of ibp domains. we have designed peptostreptococcus magnus protein l (ppl) mutants with altered immunoglobulin li ...200212351898
evaluation of serological markers to differentiate between ulcerative colitis and crohn's disease: panca, asca and agglutinating antibodies to anaerobic coccoid rods.accurate diagnosis of inflammatory bowel disease, in particular the differentiation between ulcerative colitis and crohn's disease, is important for treatment and prognosis. several serological markers have been used as non-invasive diagnostic tools in inflammatory bowel disease patients both to differentiate ulcerative colitis from crohn's disease and to define patient subgroups.200212352222
antimicrobial susceptibility of major pathogens of orofacial odontogenic infections to 11 beta-lactam this study, we evaluated the current effectiveness of 11 beta-lactam antibiotics for treatment of orofacial odontogenic infections by determining the antimicrobial susceptibility of the major pathogens. the antimicrobial susceptibilities of viridans streptococci (n = 47), peptostreptococcus (n = 67), porphyromonas (n = 18), fusobacterium (n = 57), black-pigmented prevotella (n = 59) and non-pigmented prevotella (n = 47) isolated from pus specimens of 93 orofacial odontogenic infections to pen ...200212354209
[a throat infection with rapid deterioration]. 200212362714
initial effect of controlled release chlorhexidine on subgingival microorganisms.little or no data exist on the ability of subgingival application of periochip (2.5 mg chlorhexidine gluconate in a biodegradable chip; astra pharmaceuticals, westborough, ma, usa) to suppress periodontopathic microorganisms. the present study compared the subgingival microbiota of periodontitis sites receiving the chlorhexidine chip plus scaling and root planing (sc/rp) or sc/rp alone.200212366861
sequence variations within protein families are linearly related to structural is commonly believed that similarities between the sequences of two proteins infer similarities between their structures. sequence alignments reliably recognize pairs of protein of similar structures provided that the percentage sequence identity between their two sequences is sufficiently high. this distinction, however, is statistically less reliable when the percentage sequence identity is lower than 30% and little is known then about the detailed relationship between the two measures of s ...200212381308
in vitro activities of faropenem against 579 strains of anaerobic bacteria.the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ...200212384389
recovery of anaerobic bacteria from a glossal abscess in an adolescent.glossal abscess is infrequent in children. anaerobic bacteria are rarely recovered from this infection and never have been reported in children or adolescents. a 15-year-old patient presented with a tongue abscess following trauma. aspirate of the abscess yielded polymicrobial anaerobic flora: prevotella melaninogenica, fusobacterium nucleatum, and peptostreptococcus micros. the patient recovered following incision and drainage and 14 days of antimicrobial therapy with clindamycin. this report i ...200212395007
joint and bone infections due to anaerobic bacteria in children.the current review describes the microbiology, diagnosis and management of septic arthritis and osteomyelitis due to anaerobic bacteria in children. staphylococcus aureus, haemophilus influenzae type-b, and group a streptococcus, streptococcus pneumoniae, kingela kingae, neisseria meningiditis and salmonella spp are the predominant aerobic bacteria that cause arthritis in children. gonococcal arthritis can occur in sexually active adolescents. the predominant aerobes causing osteomyelitis in chi ...200212396847
comparative in vitro activities of ertapenem against bacterial pathogens from patients with acute pelvic infection.this study compared the in vitro activities of ertapenem, ceftriaxone, co-amoxiclav, ciprofloxacin and piperacillin-tazobactam against 314 aerobic bacteria and of ertapenem, piperacillin-tazobactam, cefoxitin, ceftriaxone, chloramphenicol, ticarcillin-clavulanate, ampicillin-sulbactam, clindamycin and metronidazole against 500 anaerobic bacteria from 212 patients with acute pelvic infection. antimicrobial susceptibilities were determined by broth microdilution (aerobes) or agar dilution (anaerob ...200212407133
phenolic antibacterials from piper betle in the prevention of halitosis.piper betle l. (piperaceae) leaves which are traditionally used in india and china in the prevention of oral malodor was examined by bioassay-guided fractionation to yield allylpyrocatechol (apc) as the major active principle which showed promising activity against obligate oral anaerobes responsible for halitosis. the biological studies with apc indicated that the potential to reduce methylmercaptan and hydrogen sulfide was mainly due to the anti-microbial activity as established using dynamic ...200212413722
a study on the isolation of anaerobic bacteria from the root canal infections in anterior non-vital teeth with special reference to bacteroides species. 200212420575
prevalence of pathogens in diabetic foot infection in south indian type 2 diabetic determine the prevalence of pathogens in diabetic foot infections, in relation to parameters like wagner's grading, duration of diabetes and healing time.200212421021
two-part implants inserted in a one-stage or a two-stage procedure. a prospective comparative study.the aim of this study was to evaluate the feasibility of using a two-part implant system in a one-stage procedure and to monitor the microflora in the peri-implant area in relation to clinical and radiographic outcome.200212445222
heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16s-23s intergenic ribosomal rna polymorphisms.peptostreptococci are gram-positive, strictly anaerobic bacteria which, although regarded as members of the commensal human microflora, are also frequently isolated from sites of clinical infection. the study of this diverse group of opportunist pathogens has been hindered by an inadequate taxonomy and the lack of a valid identification scheme. recent re-classification of the peptostreptococcus family into five distinct genus groups has helped to clarify the situation. however, this has been on ...200212448679
bacteriology of acute and chronic sphenoid sinusitis.aspirates of 16 acutely infected and 7 chronically infected sphenoid sinuses were processed for aerobic and anaerobic bacteria. a total of 29 isolates were recovered from the 16 cases of acute sphenoid sinusitis (1.8 per specimen): 22 aerobic and facultative (1.4 per specimen), and 7 anaerobic (0.4 per specimen). aerobic and facultative organisms alone were recovered in 10 specimens (62%), anaerobes alone were isolated in 3 (19%), and mixed aerobic and anaerobic bacteria were recovered in 3 (19% ...200212450174
ertapenem (mk-0826), a new carbapenem: comparative in vitro activity against clinically significant anaerobes.ertapenem, a new long acting beta-lactam with broad-spectrum antimicrobial coverage, was tested in vitro to compare its activity against 556 clinical isolates of anaerobes to other established agents using a broth microdilution method as recommended by the nccls. against all anaerobes ertapenem inhibited 99.1% of isolates at 4 microg/ml, had a mode mic of 0.12 microg/ml, and showed activity comparable to imipenem, meropenem, trovafloxacin, piperacillin-tazobactam, and metronidazole. five of the ...200212458126
antibacterial activity of moxifloxacin against periodontal anaerobic pathogens involved in systemic infections.the in vitro activity of moxifloxacin was compared with that of penicillin g, amoxycillin/clavulanate, cefoxitin, erythromycin, clindamycin and metronidazole against 158 isolates associated with periodontal infections. mic(50)/mic(90) values of moxifloxacin were respectively 0.06/0.5 mg/l for porphyromonas gingivalis (35), for prevotella spp. (28) and actinomyces spp. (35), 0.12/0.25 mg/l for fusobacterium nucleatum (20) and 0.06/0.12 mg/l for peptostreptococcus spp. (30). the minimum inhibitory ...200212458140
identification of bacteria in acute endodontic infections and their antimicrobial susceptibility.the purpose of the study was to identify the bacterial composition of the microbiota from acute endodontic abscesses/cellulitis and their antimicrobial susceptibilities.200212464902
comparison of 16s rdna-based pcr and checkerboard dna-dna hybridisation for detection of selected endodontic pathogens.molecular methods have been used recently to investigate the bacteria encountered in human endodontic infections. the aim of the present study was to compare the ability of a 16s rdna-based pcr assay and checkerboard dna-dna hybridisation in detecting actinobacillus actinomycetemcomitans, bacteroides forsythus, peptostreptococcus micros, porphyromonas endodontalis, por. gingivalis and treponema denticola directly from clinical samples. specimens were obtained from 50 cases of endodontic infectio ...200212466407
development of a pcr assay specific for peptostreptococcus anaerobius.peptostreptococcus anaerobius is a gram-positive anaerobic coccus that is widely distributed in the normal human flora. the organism has also been implicated as a causative agent of several systemic infections, including endocarditis and infections of the genitourinary and gastrointestinal tracts. its role in oral disease is less well defined, although it has been implicated in periodontal disease, gingivitis and root canal infections. identification of p. anaerobius in clinical samples is curre ...200212466408
porphyromonas gingivalis, bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction.bacteria play an essential role in the pathogenesis of destructive periodontal disease. it has been suggested that not all bacteria associated with periodontitis may be normal inhabitants of a periodontally healthy dentition. in particular, porphyromonas gingivalis and actinobacillus actinomycetemcomitans have been isolated infrequently from subjects without periodontitis. the aim of the present study was to compare prevalence and proportions of a number of periodontal bacteria in periodontitis ...200212472995
development of a membrane-array method for the detection of human intestinal bacteria in fecal samples.a membrane-array method was developed for the detection of human intestinal bacteria in fecal samples without using the expensive microarray-arrayer and laser-scanner. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to nitrocellulose membranes. digoxigenin (dig)-labeled 16s rdnas were amplified by polymerase chain ...200212477438
metabolism of pyrrolizidine alkaloids by peptostreptococcus heliotrinreducens and a mixed culture derived from ovine ruminal fluid.a mixed culture of ovine ruminal microbes metabolizes the macrocyclic pyrrolizidine alkaloids present in the plant senecio jacobaea, including jacobine and seneciphylline. previous attempts to identify metabolites of these alkaloids have not been successful. the objective of this study was to compare the metabolism of pyrrolizidine alkaloids by a mixed culture of ovine ruminal microbes to the metabolism of pyrrolizidine alkaloids by the known organism peptostreptococcus heliotrinreducens. p. hel ...200212488016
comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme a dehydrogenase-novel analog complexes.spectroelectrochemical and off-resonance raman indicate that substrate/product binding to medium-chain acyl-coenzyme a (coa) dehydrogenase (pmcad) results in ligand polarization and positive flavin potential shifts, which activate the enzyme for electron transfer. bacterial short-chain acyl-coa dehydrogenase (bscad) typically exhibits smaller potential shifts upon substrate/product binding that have not been linked to ligand polarization. to further investigate the roles of ligand binding and po ...200312504892
[the microorganisms associated with bacterial vaginosis as a cause of tubal sterility].bacterial vaginosis is one of the most widespread disturbances of the normal state of the vagina of women of reproductive and menopausal age. this condition is highly disregarded due to its moderate and not always clear clinical manifestation. the disturbed proportion of microorganisms in vagina is shown in the strong decrease or lack of lactobacilli and a spread of associated with bacterial vaginosis microorganisms. g. vaginalis, mobiluncis spp., bacteroides spp. (without b. fragilis), peptostr ...200212516260
dna from periodontopathogenic bacteria is immunostimulatory for mouse and human immune cells.although bacterial dna (bdna) containing unmethylated cpg motifs stimulates innate immune cells through toll-like receptor 9 (tlr-9), its precise role in the pathophysiology of diseases is still equivocal. here we examined the immunostimulatory effects of dna extracted from periodontopathogenic bacteria. a major role in the etiology of periodontal diseases has been attributed to actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and peptostreptococcus micros. we therefore isolated d ...200312540566
acute bacterial suppurative parotitis: microbiology and management.the parotid gland is the salivary gland most commonly affected by inflammation. the most common pathogens associated with acute bacterial parotitis are staphylococcus aureus and anaerobic bacteria. the predominant anaerobes include gram-negative bacilli (including pigmented prevotella and porphyromonas spp.), fusobacterium spp., and peptostreptococcus spp. streptococcus spp. (including s. pneumoniae) and gram-negative bacilli (including escherichia coli) have also been reported. gram-negative or ...200312544218
the microflora recovered from the outer-surfaces of the frialit-2 implanto-prosthetic connector.the aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. in 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 frialit-2 implants. the functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. after r ...200312562362
management of complex multi-space odontogenic infections.the successful management of multi-space orofacial odontogenic infections involves identification of the source of the infection, the anatomical spaces encountered, the predominant microorganisms that are found during the various stages of odontogenic fascial space infection, the impact of the infectious process on defense systems, the ability to use and interpret laboratory data and imaging studies, and a thorough understanding of contemporary antibiotic and supportive care. the therapeutic goa ...200212572406
immunoglobulin superantigen protein l induces il-4 and il-13 secretion from human fc epsilon ri+ cells through interaction with the kappa light chains of ige.peptostreptococcus magnus protein l is a multidomain bacterial surface protein that correlates with virulence. it consists of up to five homologous ig-binding domains (b1-b5) that interact with the variable domain of ig kappa l chains. intact protein l stimulates the synthesis and the release of il-4 and il-13 from human basophils in vitro. a protein l fragment covering the ig-binding domains b1-b4 also induced il-4 and il-13 release from basophils. there was an excellent correlation (r(s) = 0.8 ...200312574351
vaginal bacterial flora activates rat peritoneal mast cells.sixteen strains of physiological and pathological vaginal bacteria were tested for their ability to secrete histamine from rat peritoneal mast cells in vitro. we noticed that mycoplasma hominis-induced histamine release was very high (up to 53.6%). the stimulation of rat mast cells with staphylococccus cohnii, staphylococcus coagulase(-) (two strains), ureaplasma urealyticum, peptostreptococcus spp., bacteroides capillosus, staphylococcus aureus and streptococcus agalactiae resulted in lower but ...200212575924
effects of selected immunouppressive drugs on prostaglandin release, protein synthesis and cell proliferation in human gingival fibroblasts and on the growth of plaque bacteria.immunosuppressants play an essential role in transplantation therapy. in view of the side effects, e.g. gingival overgrowth, the present in vitro study was performed in order to investigate the effect of selected immunosuppressants on metabolic activities of gingival fibroblasts. furthermore, the effect on the growth of six oral microorganisms was investigated.200312578751
the consequences of suppression of anaerobic bacteria.anaerobic bacteria such as bacteroides fragilis, peptostreptococcus species, and fusobacterium species, when accompanied by aerobic bacteria or in the presence of dead tissue, can cause severe infections. this article discusses the most common type of anaerobic infection, i.e., infection after colonic contamination of the abdominal cavity and soft tissues. colonic anaerobes rarely cause infections as solitary pathogens. mixed infections of aerobes and anaerobes are treated by source control, sur ...200012594909
peptostreptococcus micros in primary endodontic infections as detected by 16s rdna-based polymerase chain reaction.a 16s rdna-based polymerase chain reaction (pcr) method was used to detect peptostreptococcus micros in primary root canal infections. samples were collected from 50 teeth having carious lesions, necrotic pulps, and different forms of periradicular diseases. dna extracted from the samples was amplified using the pcr assay, which yielded a specific fragment of p. micros 16s rdna. p. micros was detected in 6 of 22 root canals associated with asymptomatic chronic periradicular lesions (27.3%), 2 of ...200312597709
activities of garenoxacin (bms-284756) and other agents against anaerobic clinical isolates.a total of 590 clinical isolates consisting of 33 species of both gram-positive and gram-negative anaerobes were collected from nine centers in the chicago area in 1998-1999. the largest number of isolates (330 isolates, 56%) belonged to the bacteroides group. isolates were tested by agar dilution against garenoxacin (bms-284756, t-3811 me), trovafloxacin, moxifloxacin, clindamycin, imipenem, piperacillin-tazobactam, and cefoxitin. all but one species (2% of bacteroides vulgatus isolates) were f ...200312604521
[two cases of empyema caused by peptostreptococcus]. 200212611246
faropenem, a new oral penem: antibacterial activity against selected anaerobic and fastidious periodontal isolates.the in vitro activity of faropenem, an oral penem, was compared with those of penicillin, co-amoxiclav, cefoxitin, clindamycin, erythromycin and metronidazole against 106 isolates of anaerobic pathogens involved in systemic infections. the organisms tested comprised porphyromonas gingivalis (29), prevotella spp. (eight), prevotella melaninogenica (seven), prevotella intermedia (five), actinomyces spp. (25), fusobacterium nucleatum (14), peptostreptococcus spp. (11), bacteroides ureolyticus (five ...200312615878
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1999 and march 2000].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in japan since july 1982. in this paper, the results obtained in the academic year 1999 (from april 1999 to march 2000) have been summarized. two hundred seven cases were investigated, and 411 strains were isolated from 169 cases (81.6%). of those strains, 184 and 227 strains were from primary infections and postoperative infections, respectively. in p ...200212621728
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2000 and march 2001].tendency of isolated bacteria from infections in general surgery and their antimicrobial susceptibilities during the period from april 2000 to march 2001 were investigated in a multicenter study in japan, and the following results were obtained. the number of cases investigated as objectives was 234 for one year. a total of 388 strains (136 strains from primary infections and 252 strains from postoperative infections) were isolated from 165 cases (70.5% of total cases). in primary infections, an ...200212621729
[antibacterial activity of cefpodoxime against clinical isolates in 2000 and 2001].as the post-marketing surveillance of cefpodoxime proxetil (banan), mics of cefpodoxime (cpdx, an active form of banan) against 1090 clinical isolates of 22 species from 15 medical institutions all over japan from june 2000 to march 2001 were measured using the broth microdilution method approved by the japanese society of chemotherapy and compared with those of oral cephem antibacterials, cefaclor, cefdinir, cefditoren, and cefcapene. in this study, remarkable change in the activity of cpdx was ...200212621736
[identification and susceptibility to antimicrobial agents of strictly anaerobic bacteria isolated from hospitalized patients].the aim of this study was to identify anaerobic strains isolated in 2001 from clinical specimens obtained from patients of warsaw hospital and to evaluate a susceptibility of these strains to antimicrobial agents. in 2001 two hundred and twenty five clinical strains of obligate anaerobes were cultured, which were identified in the automatic atb system (biomérieux, france) using biochemical tests api 20 a. drug-susceptibility of strains was determined also in atb system with the use of atb ana st ...200212632658
zygomatic abscess complicating a huge mastoid cholesteatoma with intact eardrum. 200312646852
anaerobic cocci populating the deep tissues of chronic wounds impair cellular wound healing responses in vitro.anaerobic cocci are estimated to be present in the deep tissues of over 50% of chronic skin wounds. while the part they play in the chronicity of these wounds is uninvestigated, anaerobic cocci have previously been shown to be involved in other chronic inflammatory human conditions.200312653737
evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility.studies of the microbiota from the canals of teeth with failure of endodontic therapy have revealed that it differs markedly from that of untreated necrotic dental pulps. this study aimed to evaluate the microbiota of 30 root-filled teeth with persisting periapical lesions and to test the antibiotic susceptibility of the most prevalent species. microbial samples, isolation and speciation were done using advanced microbiologic techniques for anaerobic species. a total of 55 bacterial species were ...200312654099
bactericidal activities of methoxyfluoroquinolones gatifloxacin and moxifloxacin against aerobic and anaerobic respiratory pathogens in serum.gatifloxacin (bristol-myers squibb) and moxifloxacin (bayer) are new methoxyfluoroquinolones with broad-spectrum activity against aerobic and anaerobic pathogens of the respiratory tract. in this investigation, we analyzed the bactericidal activity in serum over time of these antimicrobials against three aerobic (streptococcus pneumoniae, haemophilus influenzae, and staphylococcus aureus) and four anaerobic (peptostreptococcus micros, peptostreptococcus magnus, fusobacterium nucleatum, and prevo ...200312654663
microorganisms from canals of root-filled teeth with periapical lesions.the objective of the present study was to identify the microbial flora within root canals of teeth with failed root-canal treatment and to determine the association of the various species with clinical features.200312656508
an immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions.we immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. the bacteria in the specimens were detected by brown-brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. seventy percent of the examined teeth showed bacteria in ...200312669880
antimicrobial properties of self-etching primer-bonding systems.self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ...200312670069
transformation of arctiin to estrogenic and antiestrogenic substances by human intestinal bacteria.after anaerobic incubation of arctiin (1) from the seeds of arctium lappa with a human fecal suspension, six metabolites were formed, and their structures were identified as (-)-arctigenin (2), (2r,3r)-2-(3',4'-dihydroxybenzyl)-3-(3",4"-dimethoxybenzyl)butyrolactone (3), (2r,3r)-2-(3'-hydroxybenzyl)-3-(3",4"-dimethoxybenzyl)butyrolactone (4), (2r,3r)-2-(3'-hydroxybenzyl)-3-(3"-hydroxy-4"-methoxybenzyl)butyrolactone (5), (2r,3r)-2-(3'-hydroxybenzyl)-3-(3",4"-dihydroxybenzyl)butyrolactone (6), and ...200312672988
specific pcr detection of peptostreptococcus magnus.peptostreptococcus magnus is the most pathogenic and one of the most common gram-positive anaerobic cocci found in human clinical specimens. the organism has been isolated in pure culture from a range of serious infections, including meningitis and endocarditis. however, isolation of peptostreptococcus magnus from the oral cavity has rarely been attempted. identification of peptostreptococcus magnus in clinical specimens is reliant upon microbiological culture and biochemical methods, which ofte ...200312676869
ultrastructural analysis of an infected collagen-coated vascular graft.the incidence of infection following arterial reconstruction using synthetic graft materials varies from less than 1 to 5%. one of three mechanisms is thought to be responsible: 1. intraoperative contamination, 2. extension from adjacent infected or colonized tissue, or 3. hematogenous or lymphogenous seeding. we present ultrastructural data of a patient with a polymicrobial graft infection due to a prostheto-enteric fistula 16 years after reconstruction of an aortobifemoral graft. the polymer s ...200312677763
differences in antimicrobial activity of four commercial 0.12% chlorhexidine mouthrinse formulations: an in vitro contact test and salivary bacterial counts evaluate the in vitro and in vivo antimicrobial activity of four commercial 0.12% chlorhexidine mouthrinses.200312694428
microbiology and management of acute suppurative thyroiditis in children.this review describes the microbiology, diagnosis and management of suppurative thyroiditis (st). staphylococcus aureus, streptococcus pyogenes, streptococcus epidermidis, and streptococcus pneumoniae, are the predominant aerobic isolates. the most common anaerobic bacteria are gram-negative bacilli and peptostreptococcus spp. agents that are rarely recovered include klebsiella spp., haemophilus influenzae, streptococcus viridans, salmonella spp., enterobacteriaceae, mycobacterium tuberculosis, ...200312697345
microbiology and management of intra-abdominal infections in children.the present review describes the microbiology, diagnosis, and management of intra-abdominal infections in children. infection generally occurs due to the entry of enteric micro-organisms into the peritoneal cavity through a defect in the wall of the intestine or other viscus as a result of obstruction, infarction, or direct trauma. mixed aerobic and anaerobic flora can be recovered from the peritoneal cavity of these patients. the predominant aerobic isolates are escherichia coli, and enterococc ...200312709135
in vitro and in vivo antibacterial activities of telithromycin.telithromycin is one of the ketolides, characterised by a 3-keto group instead of l-cladinose and a c(11)-c(12) carbamate link by an alkyl chain to a pyridinum and imidazolium ring side chain. we evaluated in vitro and in vivo antibacterial activities of telithromycin against gynaecological pathogens.200312714813
[extrinsic ureteral obstruction secondary to inflammatory gynecologic pathology].to present two cases of extrinsic ureteral obstruction secondary to gynaecological inflammatory diseases, and to perform a bibliographic review.200312731448
bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer.the goal of this study is to determine if the smear layer affects the passage of bacteria through or around obturating material as evidenced by penetration of bacteria through and out the canal. specifically, this study focused on determining the effect of the smear layer on the magnitude of bacterial penetration through the apical foramen.200312735922
[bacterial pneumonia in hiv-infected patients]patients and methods: this is a retrospective study performed on hiv-positive patients discharged from our institution from january 1993 through december 1998 with a diagnosis of bacterial pneumonia. cases of tb or atypical micobacterial infection were excluded from this analysis. causative organisms were identified, when possible, by taking into account positive cultures from diverse sources (blood, sputum, pleural fluid and others). results: in the 6-yr period we considered, 120 patients were ...199912748443
in vitro activities of telithromycin and 10 oral agents against aerobic and anaerobic pathogens isolated from antral puncture specimens from patients with sinusitis.a study of the comparative in vitro activity of telithromycin, a new ketolide, against 155 aerobic and 171 anaerobic antral sinus puncture isolates showed it to be active against a broad range of sinus pathogens. all pneumococci, including erythromycin-resistant strains, were susceptible to telithromycin at < or = 0.5 microg/ml; all haemophilus influenzae and eikenella corrodens strains were inhibited by < or = 4 microg of telithromycin/ml; all moraxella spp. and beta-lactamase-producing prevote ...200312760875
in vitro activities of dalbavancin and nine comparator agents against anaerobic gram-positive species and corynebacteria.dalbavancin is a novel semisynthetic glycopeptide with enhanced activity against gram-positive species. its comparative in vitro activities and those of nine comparator agents, including daptomycin, vancomycin, linezolid, and quinupristin-dalfopristin, against 290 recent gram-positive clinical isolates strains, as determined by the nccls agar dilution method, were studied. the mics of dalbavancin at which 90% of various isolates tested were inhibited were as follows: actinomyces spp., 0.5 microg ...200312760876
[utility of anaerobic blood cultures in extra-hospitalary bacteremias].to compare the rentability of anaerobic hemocultures with aerobic cultures in patients suffering bacteremia, trying to analyze the rentability of anaerobic cultures.200312768830
immunoglobulin-binding domains: protein l from peptostreptococcus magnus.protein l is a multidomain cell-wall protein isolated from peptostreptococcus magnus. it belongs to a group of proteins that contain repeated domains that are able to bind to igs without stimulating an immune response, the most characterized of this group being protein a ( staphylococcus aureus ) and protein g ( streptococcus ). both of these proteins bind predominantly to the interface of c(h)2-c(h)3 heavy chains, while protein l binds exclusively to the v(l) domain of the kappa -chain. the fun ...200312773190
[early diagnosis of bacterial brain abscesses: interest of diffusion-weighted mri].three cases of bacterial brain abscesses, in immunocompetent patients, are reported. in all these cases, the diffusion-weighted magnetic resonance (mri) with apparent diffusion coefficient (adc) map has permitted an early diagnosis and a rapid treatment. this emergency mri showed in the three cases a low signal on ti-weighted images, a high signal on t2-weighted and echo-planar images, and a decrease of adc (0.36- 0.49 x 10(-3) mm2/s). so, this new mri technique provides an available and rapid e ...200312773871
deep neck infection: a present-day complication. a retrospective review of 83 cases (1998-2001).deep neck infections are less and less frequent today than in the past. nevertheless, their complications are often life-threatening. the present study reviews the experience of the department of otolaryngology and head and neck surgery of padua with deep neck infections during the period from 1998 to 2001. eighty-three patients (55 males and 28 females) were retrospectively considered. the site of origin of deep neck infection was identified in 76 patients (91%). the most common cause was denta ...200312774234
additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial is an established procedure to use calcium hydroxide [ca(oh)2] as a therapeutic component to achieve sterilization of infected root canals. unfortunately, some bacterial species are relatively resistant to ca(oh)2 and are therefore associated with treatment failures. the objective of this study was to identify combinations of substances that improve antimicrobial activity. ca(oh)2 was used as a suspension or as a component of gutta-percha points. efficacy of killing was compared for (a) ca(oh ...200312775007
late infection in patients with scoliosis treated with spinal instrumentation.sixty patients were treated using a multilevel spinal instrumentation system. spine arthrodesis was done posteriorly in all patients using a combination of two rods, hooks, screws, and cross-link plates. the galveston technique was used in three patients. five patients presented with late deep wound infections 1 to 5 years postoperatively. two patients presented with a local subcutaneous abscess, whereas the remaining patients had a local drainage. exploration revealed pus lining the instrumenta ...200312782866
evaluation of antibacterial activity of mangifera indica on anaerobic dental microglora based on in vivo studies.the present study was carried out to compare the subgingival plaque microflora in mango leaf users. fifty subjects of both sexes, 25 of them used tooth brush and 25 used mango leaf as their home care hygiene device were included in the study. the microbiological evaluation for specific bacterial counts of actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum and peptostreptococcus micros were carried out for all subject. specific microbial ...200212785172
the effects of a triclosan/copolymer dentifrice on oral bacteria including those producing hydrogen sulfide.a clinical procedure was developed to examine the effects of short-term and extended use of a triclosan/copolymer dentifrice and a commercial fluoride dentifrice on oral bacteria, including those producing hydrogen sulfide. healthy adults volunteered for this double-blind, crossover design clinical study and provided saliva samples for culturing on enriched and indicator media to enumerate all salivary bacteria and those producing hydrogen sulfide (odorigenic), respectively. subjects brushed wit ...200312786953
comparative in vitro activity of ph-027 versus linezolid and other anti-anaerobic antimicrobials against clinical isolates of clostridium difficile and other anaerobic is a new 5-triazole oxazolidinone synthesized in our laboratories, which shows strong activity against gram-positive aerobic bacteria including clinical isolates. the objective of this study was to investigate the in vitro activity of this compound in comparison with linezolid and other antibiotics against gram-positive and gram-negative anaerobes. the in vitro activity of ph-027 in comparison with those of linezolid and other antimicrobial agents was evaluated against 201 clinical isolat ...200312797385
endocarditis due to peptostreptococcus anaerobius: case report and literature review of peptostreptococcal endocarditis.infective endocarditis caused by anaerobic streptococci occurs rarely, and only five cases have been described in detail, with two additional cases mentioned in a review of anaerobic infective arthritis. all have resulted from infection caused by peptostreptococci. another case of anaerobic gram-positive endocarditis caused by peptostreptococcus anaerobius has recently been encountered. the patient was successfully treated with a beta-lactam for six weeks, plus gentamicin (to which the organism ...200312803344
increased antimicrobial resistance in organisms recovered from otitis media with effusion.previous studies concerning the microbiology of otitis media with effusion (ome) did not correlate the past use of antimicrobial agents with the recovered organism's antimicrobial susceptibility. a retrospective analysis of cultures obtained from aspirates of 129 children with ome was performed. the study identified the isolated organisms and determined their susceptibility to the most recently administered antimicrobials. bacterial growth was noted in 58 (45 per cent) patients. aerobic organism ...200312818052
antibacterial activity of medicinal plant extracts against periodontopathic bacteria.this study was performed to evaluate the antibacterial activity of althaea officinalis l. roots, arnica montana l. flowers, calendula officinalis l. flowers, hamamelis virginiana l. leaves, illicium verum hook. fruits and melissa officinalis l. leaves, against anaerobic and facultative aerobic periodontal bacteria: porphyromonas gingivalis, prevotella spp., fusobacterium nucleatum, capnocytophaga gingivalis, veilonella parvula, eikenella corrodens, peptostreptococcus micros and actinomyces odont ...200312820224
in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, bacitracin, and four other antimicrobials against intestinal anaerobic using an agar dilution method, the in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, and five other agents were determined against 300 gram-positive and 54 gram-negative strains of intestinal anaerobes. ramoplanin was active at <or=2 microg/ml against 287 of 300 (95.7%) gram-positive organisms, including 18 strains of clostridium difficile for which mics of ramoplanin were 0.25 to 0.5 microg/ml; for 3 of these, linezolid mics were 8 to 16 micro g/ml. nineteen clostridium ...200312821492
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2001 and march 2002].isolated bacteria from infections in general surgery during the period from april 2001 to march 2002 were investigated in a multicenter study in japan, and the following results were obtained. in this series, four hundred and twenty strains were isolated from 175 (79.2%) of 221 patients with surgical infections. one hundred and eighty-six strains were isolated from primary infections, and 234 strains were isolated from postoperative infections. from primary infections, anaerobic gram-positive ba ...200312825413
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