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profile of subgingival microbiota in children with mixed dentition.a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...200011155173
growth inhibitory activity of gutta-percha points containing root canal medications on common endodontic bacterial pathogens as determined by an optimized quantitative in vitro assay.gutta-percha points containing calcium hydroxide, zinc oxide (zno), a mixture of zno and chlorhexidine (zno/chx), iodine-polyvinylpyrrolidone (zno/j-pvp), or a mixture of chx and j-pvp and zno (zno/chx/j-pvp) were tested for their ability to inhibit growth of pure cultures of bacterial species commonly involved in endodontic infections (peptostreptococcus micros, streptococcus intermedius, enterococcus faecalis, and porphyromonas gingivalis). to quantitate growth inhibition, an in vitro assay wa ...200011199764
molecular epidemiology and association of putative pathogens in root canal infection.the purpose of this study was to investigate the epidemiology of putative pathogens in root canals with apical periodontitis and to determine the associations among the putative pathogens. eighteen symptomatic and 20 asymptomatic teeth from 36 subjects were studied. this research was performed with polymerase chain reaction and hybridization using rrna-based oligonucleotide probes. the most frequently found species was fusobacterium sp. (68.4%), followed by peptostreptococcus micros (44.7%) and ...200011199802
aerobic and anaerobic microbiology of wound infection following spinal fusion in children.to study the aerobic and anaerobic microbiology of wound infections following spinal fusion in children.200010765134
an experimental mouse model to study the pathogenicity of prevotella bivia and investigations of possible virulence.induction of subcutaneous abscesses in mice was used to study the pathogenicity of prevotella bivia both in mono-infection and in mixed cultures with escherichia coli and peptostreptococcus spp. virulence factors such as coaggregation and aggregate formation of cells, haemagglutination activity and tolerance to serum bactericidal activity were investigated for their possible role in p bivia pathogenicity. monocultures of p bivia, e coli and peptostreptococcus spp did not induce subcutaneous absc ...200010786446
profile of subgingival microbiota in children with primary dentition.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ...200010791707
a breakdown of symmetry in the folding transition state of protein l.the 62 residue igg binding domain of protein l consists of a central alpha-helix packed on a four-stranded beta-sheet formed by n and c-terminal beta-hairpins. the overall topology of the protein is quite symmetric: the beta-hairpins have similar lengths and make very similar interactions with the central helix. characterization of the effects of 70 point mutations distributed throughout the protein on the kinetics of folding and unfolding reveals that this symmetry is completely broken during f ...200010801362
[otogenic cerebral abscess: a persistent problem].a young man with otitis media and cholesteatoma of the left ear developed secondary cerebral abscess. the clinical debut was non-specific, with headache, mild fever, and mild persistent otalgia in spite of early antibiotic treatment. studies revealed a cerebral abscess, so ent surgery in collaboration with the neurosurgery department was decided. this case illustrates that clinical manifestations in such cases can be mild and highlights the need to exclude this type of serious pathology.200010804119
dynamics of nasopharyngitis in children.our goal was to characterize the dynamics and bacterial interaction of the aerobic and anaerobic flora of nasal discharge of children at different stages of uncomplicated nasopharyngitis. methods and patients: serial semiquantitative nasopharyngeal (np) and quantitative nasal discharge (nd) cultures were taken every 3 to 5 days from 20 children in whom purulent discharge eventually developed (group 1), and a single culture was obtained from a group of 20 who had only clear discharge (group 2).200010793349
comparative activity of moxifloxacin in vitro against obligately anaerobic bacteria.the antimicrobial activity of moxifloxacin and seven other antibiotics (four of them quinolones) against 292 strains of obligately anaerobic bacteria was assessed employing a broth microdilution technique performed in wilkens-chalgren broth. mic50/mic90 values (mg/l) for moxifloxacin were as follows: bacteroides fragilis (n = 62) 0.25/2, bacteroides ovatus (n = 70) 1/4, bacteroides vulgatus (n = 29) 0.25/1, bacteroides thetaiotaomicron (n = 17) 2/2, bacteroides caccae (n = 11) 1/2, prevotella sp ...200010795599
translocation of indigenous microflora in an experimental model of sepsis.translocation of viable bacteria from gut to bloodstream and other sterile body sites during shock has been demonstrated in several experimental and clinical studies. the factors causing translocation and its incidence at different stages of shock are not known. the aim of the study was to evaluate the importance of several factors causing translocation of indigenous microflora in an experimental model of septic shock based on intraperitoneal escherichia coli sepsis in rats. counts of inoculated ...200010798556
features of severe periodontal disease in a teenager with chédiak-higashi syndrome.chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis.200010872965
in vitro inhibition of commercial douche products against vaginal microflora.recently, vaginal douching has been associated with many health risks in women. the aim of this study was to analyze the effect of commercial douche products against various vaginal microorganisms, including lactobacilli. seven commercial douches were tested against eight lactobacillus clinical isolates and three type strains from the american type culture collection. bv-associated bacteria included six strains of five genera: gardnerella, mobiluncus, mycoplasma, peptostreptococcus, and ureaplas ...200010805365
microbiology of infected poison ivy dermatitis.we report the aerobic and anaerobic microbiology of secondarily infected poison ivy dermatitis. the study involved retrospective review of clinical and microbiology laboratory records of patients with secondarily infected poison ivy lesions. bacterial growth was noted in 33 specimens. aerobic or facultative anaerobic bacteria only were present in 18 (55%) patients, anaerobic bacteria only in seven (21%), and mixed anaerobic-aerobic bacteria in eight (24%). forty-five isolates were recovered (1.4 ...200010809852
[what is your diagnosis? bilateral abscess forming pneumonia]. 200010815462
strategy for 3-d computed tomography diagnosis and treatment of small pulmonary arteriovenous fistula.in a patient with left hemianopsia, computed tomography (ct) and magnetic resonance imaging revealed an intracranial space-occupying lesion in the right occipital region. a cerebral abscess was removed at craniotomy. enhanced pulmonary ct showed a small coin lesion in the peripheral lingula. and at 3-d ct two nidi with feeding arteries and draining veins were seen, indicating pulmonary arteriovenous fistula. lingular segmentectomy was performed.200010816068
ulcus vulvae acutum.an acute vulvar ulcer was diagnosed based on a case history of a 17-year-old girl with genital ulcers. no relevant causative factors could be established. the gangrenous form of the disease, cutaneous anergy, and the cultured anaerobic pathogens suggested the possibility of an infectious pathogenesis.200010879308
review of the in vitro activity of gemifloxacin against gram-positive and gram-negative anaerobic pathogens.published reports on the in vitro activity of gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, against anaerobic pathogens are reviewed here. the studies used a variety of media, inocula and antimicrobial agents. using a proposed breakpoint of 0.5 mg/l, these studies showed that gemifloxacin had generally higher potency against gram-positive anaerobes (clostridium perfringens, all peptostreptococcus spp.) and fusobacteria (fusobacterium nucleatum, fusobacterium necrophorum) and mode ...200010824034
in vitro activities of mk-826 (l-749,345) against 363 strains of anaerobic bacteria.the activity of mk-826 was compared to the activities of cefoxitin, ceftriaxone, imipenem, and meropenem against 363 gram-negative and gram-positive anaerobes by using nccls procedures. at least 98% of the strains were susceptible to the carbapenems. all strains of clostridium perfringens, fusobacterium nucleatum, peptostreptococcus, and sutterella wadsworthensis were susceptible to all agents tested.200010898709
toll-like receptor 4-deficient mice have reduced bone destruction following mixed anaerobic infection.c3h/hej mice have an impaired ability to respond to lipopolysaccharide (lps) due to a mutation in the gene that encodes toll-like receptor 4 (tlr4). the effect of tlr4 deficiency on host responses to endodontic infections is unknown. in the present study, we compared periapical bone destruction, sepsis, and inflammatory cytokine production in lps-hyporesponsive c3h/hej and wild-type control c3h/heouj mice. the mandibular first molars of both strains were subjected to pulpal exposure and infectio ...200010899873
interleukin-1 receptor signaling rather than that of tumor necrosis factor is critical in protecting the host from the severe consequences of a polymicrobe anaerobic infection.infection of the dental pulp leads to an osteolytic lesion that results from a polymicrobial infection consisting largely of pathogenic anaerobes. infection causes significant morbidity and mortality mediated by bacterial factors and in some cases by the up-regulation of inflammatory cytokines. the inflammatory cytokines interleukin-1 (il-1) and tumor necrosis factor (tnf), in particular, play a complex and central role in the responses to microbial pathogens. however, relatively little is known ...200010899881
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1997 and march 1998].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in 19 facilities in japan since july 1982. this paper describes the results obtained during the period from april 1997 to march 1998. the number of cases investigated as objectives was 215 for one year. a total of 420 strains (170 strains from primary infections and 250 strains from postoperative infections) were isolated from 174 cases (80.9% of total ...200011070817
linezolid.linezolid is an oxazolidinone antibacterial agent that acts by inhibiting the initiation of bacterial protein synthesis. cross-resistance between linezolid and other inhibitors of protein synthesis has not been demonstrated. linezolid has a wide spectrum of activity against gram-positive organisms including methicillin-resistant staphylococci, penicillin-resistant pneumococci and vancomycin-resistant enterococcus faecalis and e. faecium. anerobes such as clostridium spp., peptostreptococcus spp. ...200010804037
characterization of bacterial orofacial infections using a new murine model.we devised a new murine orofacial infection model using bacteria from odontogenic infection origins and characterized the experimental infections. in this model, bacteria were injected into the submandible of mice. streptococcus constellatus and peptostreptococcus micros produced a single abscess at the injection site and their abscess-forming and lethal abilities were low: the median abscess-forming dose (af(50)) of s. constellatus and p. micros were 10(8.5-10.7)and 10(10.2-10.6)cfu/mouse, and ...200010906266
[bacterial vaginosis. epidemiology and risk factors].bacterial vaginosis (bv) is a change in vaginal ecosystem where lactobacilli dominate, flora is absent or greatly reduced, and replaced with a mixed, predominantly anaerobic flora, consisting of gardnerella vaginalis, mycoplasma hominis, mobiluncus spp, bacteroides spp, prevotela spp, peptostreptococcus spp, fusobacterium spp and porphyromonas spp. the concentration of bacteria increases from 100 to 1000 fold in women with bv compared to healthy women. bv has been formerly known as nonspecific v ...200010916461
critical role of beta-hairpin formation in protein g folding.comparison of the folding mechanisms of proteins with similar structures but very different sequences can provide fundamental insights into the determinants of protein folding mechanisms. despite very little sequence similarity, the approximately 60 residue igg binding domains of protein g and protein l both consist of a single helix packed against a four-stranded sheet formed by two symmetrically disposed beta-hairpins. we demonstrate that, as in the case of protein l, one of the two beta-turns ...200010932252
differences in the composition of the subgingival microbiota of two periodontitis populations of different geographical origin. a comparison between spain and the netherlands.the purpose of this study was to compare the subgingival microbiota of two geographically distinct patient populations using identical clinical and bacteriological methods. adult patients with a diagnosis of periodontitis were consecutively selected according to pre-defined clinical criteria. microbiological samples were taken from the deepest four sites with bleeding. the samples were plated on blood agar plates, for the determination of the total anaerobic counts and identification of specific ...200011037754
the effects of amoxicillin therapy on skin flora in infants.in order to determine the effect of amoxicillin therapy on the perineal skin microbial flora in infants, we took quantitative bacterial and fungal cultures of perineal and sternal areas from 25 infants treated with amoxicillin (40 mg/kg/day) for 10 days. specimens were obtained prior to therapy, within 3 days of conclusion of therapy, and 14-16 days later. immediately following therapy, a decline in the number of bacterial isolates occurred on both the perineum (89 to 47) and sternum (84 to 39). ...200011085662
bacteriology of histopathologically defined appendicitis in children.acute appendicitis is the most common surgical emergency in childhood. however, the pathogenesis and detailed microbiology are obscure.200011099090
microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients.polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities.200011073323
the periodontal abscess (i). clinical and microbiological findings.little information is available regarding the diagnosis and microbiology of periodontal abscesses. the aim of this descriptive clinical and microbiological study was to provide more information in order to help in the characterisation of the periodontal abscess associated to periodontitis.200010883867
[cerebral abscess in an immunocompetent patient]. 200010893748
synergic activity, for anaerobes, of trovafloxacin with clindamycin or metronidazole: chequerboard and time-kill methods.chequerboard titrations were used to test the activity of trovafloxacin, alone and in combination with clindamycin or metronidazole, against 156 gram-positive or gram-negative anaerobes, including 47 bacteroides fragilis group, 36 prevotella spp., 26 fusobacteria, 21 peptostreptococci and 26 clostridia. mic50/mic90 values (mg/l) of each drug alone against all 156 strains were: trovafloxacin, 0.5/1; clindamycin, 0.25/2; metronidazole, 1/2. synergy (fic indices </= 0.5) was seen in two strains wit ...200010797085
microbiology of healthy and diseased adenoids.to determine the qualitative and quantitative microbiology of core adenoid tissue obtained from four groups of 15 children each, with recurrent otitis media (rom), recurrent adenotonsillitis (rat), obstructive adenoid hypertrophy (oah), and occlusion or speech abnormalities (controls).200010852520
in vitro activity of telithromycin (hmr 3647) and seven other antimicrobial agents against anaerobic bacteria.we assessed the in vitro activity of telithromycin (hmr 3647) and seven other antimicrobials against 292 strains of obligately anaerobic bacteria. mics were determined with the microdilution technique and wilkins-chalgren broth according to din 58940-83. mic50/mic90s (mg/l) for telithromycin were 4/4 for bacteroides fragilis, bacteroides ovatus and bacteroides thetaiotaomicron, 2/4 for fusobacterium spp. and bilophila wadsworthia, 2/2 for bacteroides caccae, 1/4 for bacteroides vulgatus, 0.25/4 ...200010882699
[the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation].nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ...200011286181
early colonization of dental implants by putative periodontal pathogens in partially edentulous patients.there is limited scientific information available on the early colonization of the peri-implant pockets in partially edentulous individuals. knowledge about this process is one step in better understanding the etiology and pathogenesis of peri-implantitis. in this study, the early colonization of the peri-implant pockets by putative periodontal pathogens was studied in 20 partially edentulous individuals using anaerobic culture techniques. at baseline, the presence and levels of putative periodo ...200011168244
the effect of statherin and its shortened analogues on anaerobic bacteria isolated from the oral cavity.the susceptibility (mic) of 44 strains of anaerobic bacteria isolated from the oral cavity and 3 standard strains to statherin and its c-terminal fragments with sequences qyqqytf, yqqytf, qqytf, qytf and ytf was determined by means of plate dilution technique in brucella agar with 5% content of defibrinated sheep's blood, menadione and hemin. the culture was anaerobic. as shown, at concentrations from 12.5 to 100 microg/ml statherin and its c-terminal fragments inhibited the growth of anaerobic ...200011293657
comparison of microbial composition in the subgingival plaque of adult crowded versus non-crowded dental regions.it has been reported in the literature that certain species of bacteria (periodontopathogens) present in the subgingival plaque are associated with destructive periodontal disease. the purpose of this study was to investigate and compare the presence and proportional distribution of periodontopathogens in the subgingival plaque of adult crowded versus non-crowded dental regions. thirty adult patients with anterior dental crowding were selected from the orthodontic clinic of the university of pen ...200011307190
intraabdominal abscess managed successfully via the laparoscopic approach.a rare complication of laparoscopic fundoplication-an intraabdominal abscess located between the fundus and the caudate lobe of the liver-is described. a 41-year-old man had undergone a laparoscopic nissen-rossetti fundoplication for longstanding gastroesophageal reflux disease. on the 5th postoperative day, the patient's general condition became worse, and he developed intermittent-remittent fever (40 degrees c), an elevated white blood cell count (wbc), and an accelerated sedimentation rate. e ...200011265065
amoxicillin plus metronidazole in the treatment of adult periodontitis patients. a double-blind placebo-controlled study.the aim of this double-blind, parallel study was to evaluate the adjunctive effects of systemically administered amoxicillin and metronidazole in a group of adult periodontitis patients who also received supra- and subgingival debridement.200111314884
[cellulitis and necrotizing fasciitis: microbiology and pathogenesis].streptococcus pyogenes is a common cause of necrotizing cutaneous infections in otherwise healthy children and adults. several surface components are involved in the processes of adherence and invasiveness, such as protein m and capsulae. streptolysin o and other bacterial products, such as pyrogenic exotoxins, are involved in tissue injury and necrosis. toxins a and c act as superantigens and are expressed by strains associated with the toxic shock syndrome. staphylococcus aureus, alone or in a ...200111319369
effect of amoxycillin with or without clavulanate on adenoid bacterial flora.the effect of antimicrobial therapy with amoxycillin (amx) or co-amoxiclav (amc) on the adenoid bacterial flora of 45 children with recurrent otitis media (rom), scheduled for elective adenoidectomy, was studied. patients were randomized before surgery into three groups of 15, having had either no antibiotic therapy (control), or 10 days of therapy with amx or amc. core adenoid tissues were quantitatively cultured for aerobic and anaerobic bacteria. polymicrobial aerobic-anaerobic flora was pres ...200111481299
linezolid: a review of its use in the management of serious gram-positive infections.linezolid is the first of a new class of antibacterial drugs, the oxazolidinones. it has inhibitory activity against a broad range of gram-positive bacteria, including methicillin-resistant staphylococcus aureus (mrsa), glycopeptide-intermediate s. aureus (gisa), vancomycin-resistant enterococci (vre) and penicillin-resistant streptococcus pneumoniae. the drug also shows activity against certain anaerobes, including clostridium perfringens, c. difficile, peptostreptococcus spp. and bacteroidesfr ...200111324682
a microbiological study of papillon-lefévre syndrome in two patients.to analyse the microflora of subgingival plaque from patients with papillon-lefévre syndrome (pls), which is a very rare disease characterised by palmar-plantar hyperkeratosis with precocious periodontal destruction.200111328836
[soft tissue infections caused by non-group a streptococci]. 200111555914
bacteria isolated after unsuccessful endodontic treatment in a north american population.the purpose of this study was to determine the composition of the microbial flora present in teeth after the failure of root canal therapy in a north american population. these results were then compared with those of the previous scandinavian studies.200111346739
relationship of cigarette smoking to the subgingival microbiota.the relationship of cigarette smoking to the composition of the subgingival microbiota is not clear. some studies indicated higher levels of certain species in smokers, while other studies failed to detect differences in the microbiota between subjects with different smoking histories. thus, the purpose of the present investigation was to examine the prevalence, proportions and levels of the subgingival species in adult subjects who were current, past or never smokers.200111350499
vaginal microflora associated with bacterial vaginosis in nonpregnant women: reliability of sialidase detection.to determine the prevalence of gardnerella vaginalis, anaerobic bacteria and mycoplasma hominis in vaginal specimens of women with and without bacterial vaginosis (bv) as well as to determine the sensitivity and specificity of the direct sialidase assay of vaginal fluid as a rapid test for diagnosing this syndrome.200111368254
recovery of anaerobic bacteria from 3 patients with infection at a pierced body site.we describe 3 adolescents who developed infections due to anaerobes at pierced body sites: the nipple, the umbilicus, and the nasal septum. anaerobes (prevotella intermedia and peptostreptococcus anaerobius) were recovered from pure culture of specimens obtained from 1 patient with nipple infection and were mixed with aerobic bacteria in cultures of specimens obtained from 2 patients (streptococcus aureus, peptostreptococcus micros, and prevotella melaninogenica were recovered from a patient wit ...200111389513
smoking affects the subgingival microflora in periodontitis.tobacco smoking has been identified as one major risk factor for destructive periodontal disease. scaling and root planing have been shown to be less effective in smokers with periodontitis. the aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers.200111394403
in vitro activity and post-antibiotic effect of quinupristin/dalfopristin (synercid).the in vitro activities of quinupristin/dalfopristin (synercid), ampicillin, erythromycin, clarithromycin, vancomycin, teicoplanin, ciprofloxacin and tetracycline were examined and compared against 526 gram-positive bacteria. the minimal inhibitory concentrations (mics) for quinupristin/dalfopristin against staphylococcus aureus, including methicillin-resistant strains, were low (mic(90) = 0.5 mg/l), and were comparable with those of vancomycin and teicoplanin. this compound was superior to the ...200111399860
[pharmacological and pharmacokinetic properties of azithromycin (zithromac), a novel 15-membered ring macrolide antibacterial agent].azithromycin (zithromac), a 15-membered ring macrolide antibacterial agent, was approved to be manufactured in japan in march 2000. it showed good in vitro and/or in vivo antibacterial activities against staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, peptostreptococcus micros, haemophilus influenzae, moraxella catarrhalis, mycoplasma pneumoniae and chlamydia pneumoniae. its activity against h. influenzae was particularly more potent than that of currently used macrolide ...200111411344
role of aromatic stacking interactions in the modulation of the two-electron reduction potentials of flavin and substrate/product in megasphaera elsdenii short-chain acyl-coenzyme a dehydrogenase.the effects of aromatic stacking interactions on the stabilization of reduced flavin adenine dinucleotide (fad) and substrate/product have been investigated in short-chain acyl-coenzyme a dehydrogenase (scad) from megasphaera elsdenii. mutations were made at the aromatic residues phe160 and tyr366, which flank either face of the noncovalently bound flavin cofactor. the electrochemical properties of the mutants were then measured in the presence and absence of a butyryl-coa/crotonyl-coa mixture. ...200111412126
nugent score related to vaginal culture in pregnant women.to relate gram-stained smears, using the nugent criteria, to quantitative and qualitative vaginal cultures in pregnant women.200111430961
microbiological characteristics of subgingival microbiota in adult periodontitis, localized juvenile periodontitis and rapidly progressive periodontitis subjects.to describe the prevalence of the cultivable subgingival microbiota in periodontal diseases and to draw attention to the polymicrobial nature of periodontic infections.200111422244
recovery of anaerobic bacteria from four children with postthoracotomy sternal wound infection.the cases of 4 children who developed postthoracotomy sternal wound infection caused by anaerobic bacteria are presented. the predominate anaerobes were peptostreptococcus species and pigmented prevotella species. polymicrobial infection was present in all cases, and aerobic bacteria also were recovered in 2 instances. all patients responded to surgical debridement and antimicrobials effective against the isolated aerobic and anaerobic bacteria. these findings highlight the potential importance ...200111433096
chi1 torsion angle dynamics in proteins from dipolar couplings.experiments are presented for the measurement of one-bond carbon-proton dipolar coupling values at ch and ch2 ositions in 13c-labeled, approximately 50% fractionally deuterated proteins. 13cbeta-1hbeta dipolar couplings have been measured for 38 of 49 possible residues in the 63-amino-acid b1 domain of peptostreptococcal protein l in two aligning media and interpreted in the context of side-chain chi1 torsion angle dynamics. the beta protons for 18 of the 25 beta-methylene-containing amino acids ...200111448195
antibacterial activity and post-antibiotic effect of flurithromycin compared with other macrolides and penicillins against periodontal pathogens.in this study the authors examined the activity of flurithromycin compared to that of erythromycin, spiramycin and penicillin against 107 strains of various species supposed to cause periodontitis. the range of mics of flurithromycin was: < or =0.06-2 mg/l for p. gingivalis (28 isolates), 0.06-2 mg/l for p. melaninogenica (7), 0.5-4 mg/l for p. intermedia (5), 0.25-8 mg/l for prevotella sp. (8), 1-16 mg/l for f. nucleatum (14), 0.12-0.5 mg/l for w. recta (2), 0.5-32 mg/l for e. corrodens, 0.5-2 ...200111450882
[bacteriologic investigation of periodontal infections].the gingivitis and periodontitis are very common diseases in hungary: more than 80% of the adult population suffers with gingivitis and 15-20% has destructive periodontitis. the composition of peridontopathogenic bacterial flora is totally different from that of the bacterial flora causing dental infections. in periodontal infections dominantly facultative and obligatory anaerobic microorganisms occur (actinobacillus actinomycetemcomitans (a. actinomycetemcomitans), porphyromonas gingivalis (p. ...200111480243
cavernous sinus thrombophlebitis caused by sphenoid sinusitis--report of autopsy case.a 34-year-old man developed fever, headache, nausea, double vision, exophthalmus, ptosis, disturbance of vision and oculomotor nerve palsy. magnetic resonance imaging and cerebral angiography led to the clinical diagnoses of cavernous sinus thrombophlebitis and suspicion of bacterial aneurysm of the left internal carotid artery, respectively. peptostreptococcus was detected in blood culture analysis. he died 15 days after admission. systemic organs showed several septic changes. in particular, t ...200111430492
an in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.the bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.200111482142
antibacterial properties of electron beam-sterilized gutta-percha cones.the purpose of the study was to determine the effect of electron beam sterilization on gutta-percha cones (gpcs) at different times after sterilization. an agar diffusion test was used with -one aerobic bacterium (bacillus subtilis) and five oral anaerobic bacteria (fusobacterium nucleatum, peptostreptococcus micros, porphyromonas gingivalis, propionibacterium acnes, and veillonella parvula). with each microorganism 30 agar plates were prepared, evenly distributed among three groups (group 1: un ...200111487145
proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus.members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ...200111491354
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1998 and march 1999].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in japan since july 1982. this paper describes the results obtained in fiscal 1998 (from april 1998 to march 1999). the number of cases investigated as objectives was 225 for one year. a total of 429 strains (121 strains from primary infections and 308 strains from postoperative infections) were isolated from 183 cases (81.3% of total cases). in primar ...200111771334
viable bacteria in root dentinal tubules of teeth with apical periodontitis.two sets of teeth with apical periodontitis were collected at different geographic locations to study the identity of bacteria left in the root dentinal tubules. root dentin of 20 of these teeth was cultured from three locations between pulp and cementum (a, b, and c). in addition dentin from eight teeth was examined histologically. using the culturing technique bacteria were found in 77% of the dentin samples from set 1 (amsterdam) and in 87.5% of the dentin samples from set 2 (glasgow). at gre ...200111491642
untreated periodontal disease in indonesian adolescents. subgingival microbiota in relation to experienced progression of periodontitis.in an indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. at baseline the age ranged from 15 to 25 years. clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up.200111422582
mutans streptococci in subgingival plaque of treated and untreated patients with periodontitis.the etiology of root caries is thought to be identical to coronal caries, though root caries seem to be more complicated because of the higher susceptibility of exposed roots (dentin) by periodontal therapy to demineralization than intact enamel. this implies that mutans streptococci are the most likely pathogens in the development of root caries. although it is known that both the numbers of mutans streptococci and the frequency of isolation in root caries lesions are negatively correlated with ...200111422591
the clinical relevance of microbiologic testing: a comparative analysis of microbiologic samples secured from the same sites and cultured in two independent laboratories.a field study using five different private periodontal practices was conducted; it compared two microbiologic culture samples simultaneously secured from the same sites within 23 individual patients and submitted for bacterial identification and antibiotic sensitivity testing to two separate laboratories. the results from the two laboratories were often different. in no instance did both laboratories agree on the presence of identical bacterial species. when only bacteria above threshold levels ...200111490400
molecular analysis of peptostreptococcus micros isolates from patients with periodontitis.recent studies provide strong evidence implicating peptostreptococcus micros in the pathogenesis of various oral infections, including oropharyngeal abscesses and periodontal disease. to date, very little is known regarding the role of p. micros in periodontal disease. therefore, a genetic analysis was initiated to differentiate among strains of p. micros infecting periodontal patients.200111495135
molecular identification of microorganisms from endodontic infections.a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ...200111526164
bacteriology of adenoids and tonsils in children with recurrent adenotonsillitis.adenoids and tonsils electively removed from 25 children with a history of recurrent group a beta-hemolytic streptococci (gabhs) adenotonsillitis were cultured for aerobic and anaerobic bacteria. two hundred twenty-four organisms (112 aerobes and facultatives, 110 anaerobes, and 2 candida albicans) were isolated from the tonsils, and 229 (111 aerobes and facultatives and 118 anaerobes) were isolated from the adenoids. mixed infection was present in all instances, with an average of 9.1 isolates ...200111558761
[comparative study of chromatography-mass spectrography of microorganism's chemical markers in blood and intestinal mucosa bioptats].fatty acids, that are microorganism's markers in blood and jejunum's, ileum's, and large intestine's bioptats of the patients with irritable bowel syndrome were investigated with gas chromatography. markers of cl. perfringens, cl. difficile, enterococcus, streptomyces, enterobacterial, klebsiella, e. coli, peptostreptococcus, candida albicans, genus of streptococcus, of staphylococcus, of fusobacterium sp and others microorganisms were revealed. settling of intestinal mucosa was the same in jeju ...200111565126
complex between peptostreptococcus magnus protein l and a human antibody reveals structural convergence in the interaction modes of fab binding proteins.peptostreptococcus magnus protein l (ppl) is a multidomain, bacterial surface protein whose presence correlates with virulence. it consists of up to five homologous immunoglobulin binding domains that interact with the variable (vl) regions of kappa light chains found on two thirds of mammalian antibodies.200111587642
roles of native topology and chain-length scaling in protein folding: a simulation study with a go-like model.we perform folding simulations on 18 small proteins with using a simple go-like protein model and analyze the folding rate constants, characteristics of the transition state ensemble, and those of the denatured states in terms of native topology and chain length. near the folding transition temperature, the folding rate k(f) scales as k(f) approximately exp(-c rco n(0.6)) where rco and n are the relative contact order and number of residues, respectively. here the topology rco dependence of the ...200111601854
comparative in vitro activity of ertapenem and 11 other antimicrobial agents against aerobic and anaerobic pathogens isolated from skin and soft tissue animal and human bite wound infections.we studied the comparative in vitro activity of ertapenem, a new carbapenem, against 240 aerobic and 180 anaerobic recent clinical bite isolates using an agar dilution method and an inoculum of 10(4) cfu/spot for aerobes and 10(5) cfu/spot for anaerobes. ertapenem inhibited 410/420 (98%) of the isolates tested at < or = 4 mg/l with only 4/5 campylobacter gracilis and 1/3 campylobacter rectus strains requiring . or = 16 mg/l for inhibition. ertapenem was only moderately active (mic 8 mg/l) agains ...200111679553
aggressive periodontitis associated with fanconi's anemia. a case report.fanconi's anemia is an autosomal recessive disease associated with chromosomal breakage as well as pancytopenia, skin pigmentation, renal hypoplasia, cardiac defects, microcephaly, congenital malformations of the skeleton, hypogonadism, and increased risk of leukemia. the present report describes the periodontal clinical and microbiological status of an 11-year old male having fanconi's anemia.200111759873
the bacterial flora in inflammatory bowel disease: current insights in pathogenesis and the influence of antibiotics and probiotics.the pathogenesis of inflammatory bowel disease (ibd) remains unknown, although in recent years more data have become available. the contribution of genetic and environmental factors is evident, and the luminal bacterial flora plays a major role in the initiation and perpetuation of chronic ibd. animal models of ibd have shown that colitis does not occur in a germ-free environment. in human ibd, inflammation is present in parts of the gut containing the highest bacterial concentrations. moreover, ...200111768558
ertapenem: a new carbapenem.ertapenem is a new 1-beta-methyl carbapenem, stable to dehydropeptidase, which binds preferable to penicillin-binding proteins (pbp) 2 and 3. ertapenem has a broad antibacterial spectrum with mic90 values < 0.5 mg/l for penicillin-susceptible streptococcus pneumoniae, streptococcus pyogenes, methicillin-sensitive staphylococcus aureus, haemophilus influenzae, moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., serratia spp., proteus spp., clostridium perfringens, fusobact ...200111772242
[the microflora of ulzerous zone mucosa in patients with duodenal ulcer].bacteriological study of the biopsies taken from gastric and duodenal mucosa of 10 healthy volunteers and 74 patients with duodenal ulcer, was carried out. in the gastroduodenal zone of healthy subjects microorganisms of 6 genera (streptococcus, candida, staphylococcus, bacillus, helicobacter and lactobacillus) were detected. h. pylori was isolated in 20% of cases only in biopsy specimens taken from the antral section of the stomach of healthy as monoculture or in combination with c. albicans. i ...200111871291
immunoglobulin-binding domains of peptostreptococcal protein l enhance vaginal colonization of mice by streptococcus gordonii.protein l, an immunoglobulin-binding protein of some strains of the anaerobic bacterium peptostreptococcus magnus, has been hypothesized to be a virulence determinant in bacterial vaginosis. in order to investigate the role of protein l in peptostreptococcal virulence, the ig-binding domains of protein l were expressed at the surface of the human oral commensal streptococcus gordonii. recombinant streptococci were used in vaginal colonization experiments, and protein l-expressing s. gordonii dem ...200111312616
statistical theory for protein combinatorial libraries. packing interactions, backbone flexibility, and the sequence variability of a main-chain structure.combinatorial experiments provide new ways to probe the determinants of protein folding and to identify novel folding amino acid sequences. these types of experiments, however, are complicated both by enormous conformational complexity and by large numbers of possible sequences. therefore, a quantitative computational theory would be helpful in designing and interpreting these types of experiment. here, we present and apply a statistically based, computational approach for identifying the proper ...200111178917
[clinical and microbiological study of adult periodontal disease].the aim of this study was to carry out a microbiological evaluation of sites with and without clinical evidence of moderate and severe periodontitis and their correlation with clinical parameters. a total of 52 disease sites and 10 healthy sites were selected according to clinical criteria. the following clinical indexes were measured for all the sites: plaque index, gingival index, blood on probing, depth on probing and insertion level. samples of subgingival plaque were collected for culture a ...200111594003
efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters.the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition.200111686813
absence of a specific subgingival microflora in adults with down's syndrome.periodontal disease in down's syndrome (ds) is generally characterized by a high degree of bone loss. bone loss of 5 mm or more is observed in 70% of these subjects. among ds subjects, considerable differences in disease progression occur. so far, no studies have been conducted in which specific properties of the subgingival microflora have been related to the condition observed.200111686820
computer-based redesign of a protein folding pathway.a fundamental test of our current understanding of protein folding is to rationally redesign protein folding pathways. we use a computer-based design strategy to switch the folding pathway of protein g, which normally involves formation of the second, but not the first, beta-turn at the rate limiting step in folding. backbone conformations and amino acid sequences that maximize the interaction density in the first beta-hairpin were identified, and two variants containing 11 amino acid replacemen ...200111427890
correlation between microbiology and previous sinus surgery in patients with chronic maxillary sinusitis.aspirates of 108 chronically inflamed maxillary sinuses were processed for aerobic and anaerobic bacteria. there were 295 bacterial isolates: 109 aerobic and facultative, and 186 anaerobic. the predominant aerobic isolates were staphylococcus aureus (17 isolates), alpha-hemolytic streptococci (14), pseudomonas aeruginosa (12), moraxella catarrhalis (10), and haemophilus spp (8). the predominant anaerobes were peptostreptococcus spp (61), prevotella spp (45), fusobacterium spp (15), and propionib ...200111219522
microbial analysis of bone collected during implant surgery: a clinical and laboratory study.dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. however, this debris is potentially contaminated with oral bacteria. therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. twenty-four partially dentate patients were randomly al ...200111251657
multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of bacteroides fragilis group, prevotella, fusobacterium, porphyromonas, and peptostreptococcus species.in vitro surveys of antimicrobial resistance among clinically important anaerobes are an important source of information that can be used for clinical decisions in the choice of empiric antimicrobial therapy. this study surveyed the susceptibilities of 556 clinical anaerobic isolates from four large medical centers using a broth microdilution method. piperacillin-tazobactam was the only antimicrobial agent to which all the isolates were susceptible. similarly, imipenem, meropenem, and metronidaz ...200111257040
structures of the b1 domain of protein l from peptostreptococcus magnus with a tyrosine to tryptophan substitution.the three-dimensional structure of a tryptophan-containing variant of the igg-binding b1 domain of protein l has been solved in two crystal forms to 1.7 and 1.8 a resolution. in one of the crystal forms, the entire n-terminal histidine-tag region was immobilized through the coordination of zinc ions and its structural conformation along with the zinc coordination scheme were determined. however, the ordering of the histidine tag by zinc does not affect the overall structure of the rest of the pr ...200111264576
smoking and subgingival microflora in periodontal disease.the present investigation was undertaken to analyze the influence of smoking on the periodontal disease associated subgingival microflora. the population included 33 smokers and 31 non-smokers in the age range 36-86 years.200111284533
bacteriology and beta-lactamase activity in acute exacerbation of chronic bronchitis.to assess the bacteriology of beta-lactamase (bl) enzyme activity in sputum of 40 patients with acute exacerbation of chronic bronchitis (aecb).200111468101
[use of the etest method for antimicrobial susceptibility testing of obligate anaerobes].the aim of this study was to evaluate etest usefulness for antimicrobial susceptibility testing of obligate anaerobes and to compare the activity of five antibacterial drugs against clinical strains of anaerobes. one hundred strains of obligate anaerobes were tested: 2 reference strains (b. fragilis atcc 25285 and b. thetaiotaomicron atcc 29741) and 98 clinical strains isolated from patients of the infant jesus clinical hospital--center for trauma treatment in warsaw during the last three years ...200111757426
[a comparative study of various evaluation methods of the antibiotic sensitivity of strict anaerobic bacteria of the subgingival flora].the study on the sensitiveness of slow-growing anaerobes bacteria to antibiotics is delicate when you consider the technical motives that make it difficult to transpose the standard methods frequently used in microbiological laboratories. the three main methods used to determine susceptibility to antibiotics are: disk-diffusion test, antibiotics containing microdilution plates and atb ana (biomérieux). the aim of this study is to compare the effectiveness of each of these methods on severe anaer ...200111808376
identification of predominant human and animal anaerobic intestinal bacterial species by terminal restriction fragment patterns (trfps): a rapid, pcr-based method.identification of predominant human and animal intestinal tract anaerobes by conventional methods is cumbersome, time-consuming and less sensitive as compared to molecular methods. we have developed a molecular technique to identify most of the abundant intestinal microflora by polyermase chain reaction (pcr) amplification of a 16s rrna gene fragment using a pair of universal pcr primers. the forward pcr primer was labelled with 6-carboxyfluorescein amino hexy (6-fam) fluorescent dye to detect t ...200111851378
single-site mutations induce 3d domain swapping in the b1 domain of protein l from peptostreptococcus magnus.thermodynamic and kinetic studies of the protein l b1 domain (ppl) suggest a folding pathway in which, during the folding transition, the first beta hairpin is formed while the second beta hairpin and the alpha helix are largely unstructured. the same mutations in the two beta turns have opposite effects on the folding and unfolding rates. three of the four residues composing the second beta turn in ppl have consecutive positive phi angles, indicating strain in the second beta turn.200111709166
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.200111737506
supragingival and subgingival microbiota of adult patients with down's syndrome. changes after periodontal treatment.in this longitudinal study, five adult down's syndrome patients with periodontitis were placed on a frequent recall visit schedule (every 6 weeks) after treatment, in order to investigate: 1) the microbiological status, both supragingivally and subgingivally, and the changes that occurred after treatment and 2) the effect of frequent professional supragingival plaque control on the subgingival microbiota and clinical variables in these patients. the clinical variables recorded were probing pocke ...200111737662
the microbiology of mucopyocele.to evaluate the aerobic and anaerobic microbiology of mucopyocele.200111801943
splenic abscess caused by peptostreptococcus species, diagnosed with the aid of abdominal computerized tomography and treated with percutaneous drainage and antibiotics: a case report.we describe herein a 63-year-old patient with a splenic abscess due to peptostreptococcus spp., diagnosed with the aid of abdominal computerised tomography and treated with ultra-sound guided percutaneous drainage and antibiotics. the bacteriological characteristics of splenic abscesses are discussed.200111744179
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