PMID(sorted ascending)
molecular epidemiology and association of putative pathogens in root canal infection.the purpose of this study was to investigate the epidemiology of putative pathogens in root canals with apical periodontitis and to determine the associations among the putative pathogens. eighteen symptomatic and 20 asymptomatic teeth from 36 subjects were studied. this research was performed with polymerase chain reaction and hybridization using rrna-based oligonucleotide probes. the most frequently found species was fusobacterium sp. (68.4%), followed by peptostreptococcus micros (44.7%) and ...200011199802
antagonism against vibrio cholerae by diffusible substances produced by bacterial components of the human faecal microbiota.cholera vibrios sometimes survive, probably in low-level silent populations, in the small intestine of chronic carriers or pass through the gastrointestinal tract of a few individuals without causing diarrhoea or colonisation. to understand these situations, the present study used plate cultures (ex-vivo test) to investigate the frequency of appearance of an inhibitory halo against vibrio cholerae produced by faecal specimens from 92 healthy volunteers (40 females, 52 males) aged 4-61 years. the ...200111211223
correlation between microbiology and previous sinus surgery in patients with chronic maxillary sinusitis.aspirates of 108 chronically inflamed maxillary sinuses were processed for aerobic and anaerobic bacteria. there were 295 bacterial isolates: 109 aerobic and facultative, and 186 anaerobic. the predominant aerobic isolates were staphylococcus aureus (17 isolates), alpha-hemolytic streptococci (14), pseudomonas aeruginosa (12), moraxella catarrhalis (10), and haemophilus spp (8). the predominant anaerobes were peptostreptococcus spp (61), prevotella spp (45), fusobacterium spp (15), and propionib ...200111219522
detection of peptostreptococcus micros dna in clinical samples by pcr.peptostreptococcus micros is a gram-positive anaerobic coccus which, although considered to be a natural commensal of the human oral cavity, is associated with periodontal, endodontal and peritonsillar infections. identification of the organism has to date relied upon conventional culture methods and biochemical analyses. the purpose of this study was to develop a pcr method for rapid and specific identification of this organism in clinical samples. a pair of primers was selected, each of which ...200111232771
microorganisms isolated from root canals presenting necrotic pulp and their drug susceptibility in vitro.the knowledge about causative agents involved in endodontic infections is increasing, especially due to the improvement of culture techniques for anaerobic bacteria, showing that these microorganisms are predominant in this pathology. in this study, 31 canals with pulp necrosis were microbiologically analyzed before and after manipulation. obligate and facultative anaerobes, microaerophilic bacteria and yeasts were recovered from 24, 14, 5 and 2 clinical specimens, respectively. the most frequen ...200111240863
microbial analysis of bone collected during implant surgery: a clinical and laboratory implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. however, this debris is potentially contaminated with oral bacteria. therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. twenty-four partially dentate patients were randomly al ...200111251657
multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of bacteroides fragilis group, prevotella, fusobacterium, porphyromonas, and peptostreptococcus vitro surveys of antimicrobial resistance among clinically important anaerobes are an important source of information that can be used for clinical decisions in the choice of empiric antimicrobial therapy. this study surveyed the susceptibilities of 556 clinical anaerobic isolates from four large medical centers using a broth microdilution method. piperacillin-tazobactam was the only antimicrobial agent to which all the isolates were susceptible. similarly, imipenem, meropenem, and metronidaz ...200111257040
[bacteriological study of chronic maxillary sinusitis in adults and observation of susceptibility to antibiotics].the unilateral mucosal samples were taken from 42 patients and were cultured for bacteria. meanwhile, the antimicrobial susceptibility was determined for 11 antibiotics and the beta-lactamase-producing bacteria(beta lpb) were examined. in 85.71% of all samples, bacterial growth was present. anaerobic bacteria were present in 21.43% of cases. predominant aerobic bacteria were alpha-hemolytic streptococci and coagulase-negative staphylococci. predominant anaerobic bacteria were peptostreptococcus ...199811263213
structures of the b1 domain of protein l from peptostreptococcus magnus with a tyrosine to tryptophan substitution.the three-dimensional structure of a tryptophan-containing variant of the igg-binding b1 domain of protein l has been solved in two crystal forms to 1.7 and 1.8 a resolution. in one of the crystal forms, the entire n-terminal histidine-tag region was immobilized through the coordination of zinc ions and its structural conformation along with the zinc coordination scheme were determined. however, the ordering of the histidine tag by zinc does not affect the overall structure of the rest of the pr ...200111264576
intraabdominal abscess managed successfully via the laparoscopic approach.a rare complication of laparoscopic fundoplication-an intraabdominal abscess located between the fundus and the caudate lobe of the liver-is described. a 41-year-old man had undergone a laparoscopic nissen-rossetti fundoplication for longstanding gastroesophageal reflux disease. on the 5th postoperative day, the patient's general condition became worse, and he developed intermittent-remittent fever (40 degrees c), an elevated white blood cell count (wbc), and an accelerated sedimentation rate. e ...200011265065
smoking and subgingival microflora in periodontal disease.the present investigation was undertaken to analyze the influence of smoking on the periodontal disease associated subgingival microflora. the population included 33 smokers and 31 non-smokers in the age range 36-86 years.200111284533
[the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation].nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ...200011286181
the effect of statherin and its shortened analogues on anaerobic bacteria isolated from the oral cavity.the susceptibility (mic) of 44 strains of anaerobic bacteria isolated from the oral cavity and 3 standard strains to statherin and its c-terminal fragments with sequences qyqqytf, yqqytf, qqytf, qytf and ytf was determined by means of plate dilution technique in brucella agar with 5% content of defibrinated sheep's blood, menadione and hemin. the culture was anaerobic. as shown, at concentrations from 12.5 to 100 microg/ml statherin and its c-terminal fragments inhibited the growth of anaerobic ...200011293657
comparison of microbial composition in the subgingival plaque of adult crowded versus non-crowded dental has been reported in the literature that certain species of bacteria (periodontopathogens) present in the subgingival plaque are associated with destructive periodontal disease. the purpose of this study was to investigate and compare the presence and proportional distribution of periodontopathogens in the subgingival plaque of adult crowded versus non-crowded dental regions. thirty adult patients with anterior dental crowding were selected from the orthodontic clinic of the university of pen ...200011307190
immunoglobulin-binding domains of peptostreptococcal protein l enhance vaginal colonization of mice by streptococcus gordonii.protein l, an immunoglobulin-binding protein of some strains of the anaerobic bacterium peptostreptococcus magnus, has been hypothesized to be a virulence determinant in bacterial vaginosis. in order to investigate the role of protein l in peptostreptococcal virulence, the ig-binding domains of protein l were expressed at the surface of the human oral commensal streptococcus gordonii. recombinant streptococci were used in vaginal colonization experiments, and protein l-expressing s. gordonii dem ...200111312616
amoxicillin plus metronidazole in the treatment of adult periodontitis patients. a double-blind placebo-controlled study.the aim of this double-blind, parallel study was to evaluate the adjunctive effects of systemically administered amoxicillin and metronidazole in a group of adult periodontitis patients who also received supra- and subgingival debridement.200111314884
[cellulitis and necrotizing fasciitis: microbiology and pathogenesis].streptococcus pyogenes is a common cause of necrotizing cutaneous infections in otherwise healthy children and adults. several surface components are involved in the processes of adherence and invasiveness, such as protein m and capsulae. streptolysin o and other bacterial products, such as pyrogenic exotoxins, are involved in tissue injury and necrosis. toxins a and c act as superantigens and are expressed by strains associated with the toxic shock syndrome. staphylococcus aureus, alone or in a ...200111319369
linezolid: a review of its use in the management of serious gram-positive infections.linezolid is the first of a new class of antibacterial drugs, the oxazolidinones. it has inhibitory activity against a broad range of gram-positive bacteria, including methicillin-resistant staphylococcus aureus (mrsa), glycopeptide-intermediate s. aureus (gisa), vancomycin-resistant enterococci (vre) and penicillin-resistant streptococcus pneumoniae. the drug also shows activity against certain anaerobes, including clostridium perfringens, c. difficile, peptostreptococcus spp. and bacteroidesfr ...200111324682
a microbiological study of papillon-lefévre syndrome in two analyse the microflora of subgingival plaque from patients with papillon-lefévre syndrome (pls), which is a very rare disease characterised by palmar-plantar hyperkeratosis with precocious periodontal destruction.200111328836
bacteria isolated after unsuccessful endodontic treatment in a north american population.the purpose of this study was to determine the composition of the microbial flora present in teeth after the failure of root canal therapy in a north american population. these results were then compared with those of the previous scandinavian studies.200111346739
relationship of cigarette smoking to the subgingival microbiota.the relationship of cigarette smoking to the composition of the subgingival microbiota is not clear. some studies indicated higher levels of certain species in smokers, while other studies failed to detect differences in the microbiota between subjects with different smoking histories. thus, the purpose of the present investigation was to examine the prevalence, proportions and levels of the subgingival species in adult subjects who were current, past or never smokers.200111350499
comparative in vitro activities of gemifloxacin, other quinolones, and nonquinolone antimicrobials against obligately anaerobic bacteria.the in vitro activity of gemifloxacin was compared to that of other quinolone and nonquinolone antimicrobials against 204 anaerobes by the agar dilution technique. the data indicate that gemifloxacin has a rather selective anaerobic activity. most peptostreptococcus, porphyromonas, and fusobacterium species are susceptible, while gemifloxacin's activity against other gram-negative anaerobes appears to be variable.200111353648
vaginal microflora associated with bacterial vaginosis in nonpregnant women: reliability of sialidase determine the prevalence of gardnerella vaginalis, anaerobic bacteria and mycoplasma hominis in vaginal specimens of women with and without bacterial vaginosis (bv) as well as to determine the sensitivity and specificity of the direct sialidase assay of vaginal fluid as a rapid test for diagnosing this syndrome.200111368254
recovery of anaerobic bacteria from 3 patients with infection at a pierced body site.we describe 3 adolescents who developed infections due to anaerobes at pierced body sites: the nipple, the umbilicus, and the nasal septum. anaerobes (prevotella intermedia and peptostreptococcus anaerobius) were recovered from pure culture of specimens obtained from 1 patient with nipple infection and were mixed with aerobic bacteria in cultures of specimens obtained from 2 patients (streptococcus aureus, peptostreptococcus micros, and prevotella melaninogenica were recovered from a patient wit ...200111389513
smoking affects the subgingival microflora in periodontitis.tobacco smoking has been identified as one major risk factor for destructive periodontal disease. scaling and root planing have been shown to be less effective in smokers with periodontitis. the aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers.200111394403
in vitro activity and post-antibiotic effect of quinupristin/dalfopristin (synercid).the in vitro activities of quinupristin/dalfopristin (synercid), ampicillin, erythromycin, clarithromycin, vancomycin, teicoplanin, ciprofloxacin and tetracycline were examined and compared against 526 gram-positive bacteria. the minimal inhibitory concentrations (mics) for quinupristin/dalfopristin against staphylococcus aureus, including methicillin-resistant strains, were low (mic(90) = 0.5 mg/l), and were comparable with those of vancomycin and teicoplanin. this compound was superior to the ...200111399860
[pharmacological and pharmacokinetic properties of azithromycin (zithromac), a novel 15-membered ring macrolide antibacterial agent].azithromycin (zithromac), a 15-membered ring macrolide antibacterial agent, was approved to be manufactured in japan in march 2000. it showed good in vitro and/or in vivo antibacterial activities against staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, peptostreptococcus micros, haemophilus influenzae, moraxella catarrhalis, mycoplasma pneumoniae and chlamydia pneumoniae. its activity against h. influenzae was particularly more potent than that of currently used macrolide ...200111411344
role of aromatic stacking interactions in the modulation of the two-electron reduction potentials of flavin and substrate/product in megasphaera elsdenii short-chain acyl-coenzyme a dehydrogenase.the effects of aromatic stacking interactions on the stabilization of reduced flavin adenine dinucleotide (fad) and substrate/product have been investigated in short-chain acyl-coenzyme a dehydrogenase (scad) from megasphaera elsdenii. mutations were made at the aromatic residues phe160 and tyr366, which flank either face of the noncovalently bound flavin cofactor. the electrochemical properties of the mutants were then measured in the presence and absence of a butyryl-coa/crotonyl-coa mixture. ...200111412126
microbiological characteristics of subgingival microbiota in adult periodontitis, localized juvenile periodontitis and rapidly progressive periodontitis describe the prevalence of the cultivable subgingival microbiota in periodontal diseases and to draw attention to the polymicrobial nature of periodontic infections.200111422244
untreated periodontal disease in indonesian adolescents. subgingival microbiota in relation to experienced progression of an indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. at baseline the age ranged from 15 to 25 years. clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up.200111422582
mutans streptococci in subgingival plaque of treated and untreated patients with periodontitis.the etiology of root caries is thought to be identical to coronal caries, though root caries seem to be more complicated because of the higher susceptibility of exposed roots (dentin) by periodontal therapy to demineralization than intact enamel. this implies that mutans streptococci are the most likely pathogens in the development of root caries. although it is known that both the numbers of mutans streptococci and the frequency of isolation in root caries lesions are negatively correlated with ...200111422591
computer-based redesign of a protein folding pathway.a fundamental test of our current understanding of protein folding is to rationally redesign protein folding pathways. we use a computer-based design strategy to switch the folding pathway of protein g, which normally involves formation of the second, but not the first, beta-turn at the rate limiting step in folding. backbone conformations and amino acid sequences that maximize the interaction density in the first beta-hairpin were identified, and two variants containing 11 amino acid replacemen ...200111427890
cavernous sinus thrombophlebitis caused by sphenoid sinusitis--report of autopsy case.a 34-year-old man developed fever, headache, nausea, double vision, exophthalmus, ptosis, disturbance of vision and oculomotor nerve palsy. magnetic resonance imaging and cerebral angiography led to the clinical diagnoses of cavernous sinus thrombophlebitis and suspicion of bacterial aneurysm of the left internal carotid artery, respectively. peptostreptococcus was detected in blood culture analysis. he died 15 days after admission. systemic organs showed several septic changes. in particular, t ...200111430492
nugent score related to vaginal culture in pregnant relate gram-stained smears, using the nugent criteria, to quantitative and qualitative vaginal cultures in pregnant women.200111430961
recovery of anaerobic bacteria from four children with postthoracotomy sternal wound infection.the cases of 4 children who developed postthoracotomy sternal wound infection caused by anaerobic bacteria are presented. the predominate anaerobes were peptostreptococcus species and pigmented prevotella species. polymicrobial infection was present in all cases, and aerobic bacteria also were recovered in 2 instances. all patients responded to surgical debridement and antimicrobials effective against the isolated aerobic and anaerobic bacteria. these findings highlight the potential importance ...200111433096
chi1 torsion angle dynamics in proteins from dipolar couplings.experiments are presented for the measurement of one-bond carbon-proton dipolar coupling values at ch and ch2 ositions in 13c-labeled, approximately 50% fractionally deuterated proteins. 13cbeta-1hbeta dipolar couplings have been measured for 38 of 49 possible residues in the 63-amino-acid b1 domain of peptostreptococcal protein l in two aligning media and interpreted in the context of side-chain chi1 torsion angle dynamics. the beta protons for 18 of the 25 beta-methylene-containing amino acids ...200111448195
antibacterial activity and post-antibiotic effect of flurithromycin compared with other macrolides and penicillins against periodontal this study the authors examined the activity of flurithromycin compared to that of erythromycin, spiramycin and penicillin against 107 strains of various species supposed to cause periodontitis. the range of mics of flurithromycin was: < or =0.06-2 mg/l for p. gingivalis (28 isolates), 0.06-2 mg/l for p. melaninogenica (7), 0.5-4 mg/l for p. intermedia (5), 0.25-8 mg/l for prevotella sp. (8), 1-16 mg/l for f. nucleatum (14), 0.12-0.5 mg/l for w. recta (2), 0.5-32 mg/l for e. corrodens, 0.5-2 ...200111450882
bacteriology and beta-lactamase activity in acute exacerbation of chronic assess the bacteriology of beta-lactamase (bl) enzyme activity in sputum of 40 patients with acute exacerbation of chronic bronchitis (aecb).200111468101
[bacteriologic investigation of periodontal infections].the gingivitis and periodontitis are very common diseases in hungary: more than 80% of the adult population suffers with gingivitis and 15-20% has destructive periodontitis. the composition of peridontopathogenic bacterial flora is totally different from that of the bacterial flora causing dental infections. in periodontal infections dominantly facultative and obligatory anaerobic microorganisms occur (actinobacillus actinomycetemcomitans (a. actinomycetemcomitans), porphyromonas gingivalis (p. ...200111480243
effect of amoxycillin with or without clavulanate on adenoid bacterial flora.the effect of antimicrobial therapy with amoxycillin (amx) or co-amoxiclav (amc) on the adenoid bacterial flora of 45 children with recurrent otitis media (rom), scheduled for elective adenoidectomy, was studied. patients were randomized before surgery into three groups of 15, having had either no antibiotic therapy (control), or 10 days of therapy with amx or amc. core adenoid tissues were quantitatively cultured for aerobic and anaerobic bacteria. polymicrobial aerobic-anaerobic flora was pres ...200111481299
an in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.the bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.200111482142
antibacterial properties of electron beam-sterilized gutta-percha cones.the purpose of the study was to determine the effect of electron beam sterilization on gutta-percha cones (gpcs) at different times after sterilization. an agar diffusion test was used with -one aerobic bacterium (bacillus subtilis) and five oral anaerobic bacteria (fusobacterium nucleatum, peptostreptococcus micros, porphyromonas gingivalis, propionibacterium acnes, and veillonella parvula). with each microorganism 30 agar plates were prepared, evenly distributed among three groups (group 1: un ...200111487145
the clinical relevance of microbiologic testing: a comparative analysis of microbiologic samples secured from the same sites and cultured in two independent laboratories.a field study using five different private periodontal practices was conducted; it compared two microbiologic culture samples simultaneously secured from the same sites within 23 individual patients and submitted for bacterial identification and antibiotic sensitivity testing to two separate laboratories. the results from the two laboratories were often different. in no instance did both laboratories agree on the presence of identical bacterial species. when only bacteria above threshold levels ...200111490400
proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus.members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ...200111491354
viable bacteria in root dentinal tubules of teeth with apical periodontitis.two sets of teeth with apical periodontitis were collected at different geographic locations to study the identity of bacteria left in the root dentinal tubules. root dentin of 20 of these teeth was cultured from three locations between pulp and cementum (a, b, and c). in addition dentin from eight teeth was examined histologically. using the culturing technique bacteria were found in 77% of the dentin samples from set 1 (amsterdam) and in 87.5% of the dentin samples from set 2 (glasgow). at gre ...200111491642
molecular analysis of peptostreptococcus micros isolates from patients with periodontitis.recent studies provide strong evidence implicating peptostreptococcus micros in the pathogenesis of various oral infections, including oropharyngeal abscesses and periodontal disease. to date, very little is known regarding the role of p. micros in periodontal disease. therefore, a genetic analysis was initiated to differentiate among strains of p. micros infecting periodontal patients.200111495135
molecular identification of microorganisms from endodontic infections.a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ...200111526164
[soft tissue infections caused by non-group a streptococci]. 200111555914
bacteriology of adenoids and tonsils in children with recurrent adenotonsillitis.adenoids and tonsils electively removed from 25 children with a history of recurrent group a beta-hemolytic streptococci (gabhs) adenotonsillitis were cultured for aerobic and anaerobic bacteria. two hundred twenty-four organisms (112 aerobes and facultatives, 110 anaerobes, and 2 candida albicans) were isolated from the tonsils, and 229 (111 aerobes and facultatives and 118 anaerobes) were isolated from the adenoids. mixed infection was present in all instances, with an average of 9.1 isolates ...200111558761
[comparative study of chromatography-mass spectrography of microorganism's chemical markers in blood and intestinal mucosa bioptats].fatty acids, that are microorganism's markers in blood and jejunum's, ileum's, and large intestine's bioptats of the patients with irritable bowel syndrome were investigated with gas chromatography. markers of cl. perfringens, cl. difficile, enterococcus, streptomyces, enterobacterial, klebsiella, e. coli, peptostreptococcus, candida albicans, genus of streptococcus, of staphylococcus, of fusobacterium sp and others microorganisms were revealed. settling of intestinal mucosa was the same in jeju ...200111565126
complex between peptostreptococcus magnus protein l and a human antibody reveals structural convergence in the interaction modes of fab binding proteins.peptostreptococcus magnus protein l (ppl) is a multidomain, bacterial surface protein whose presence correlates with virulence. it consists of up to five homologous immunoglobulin binding domains that interact with the variable (vl) regions of kappa light chains found on two thirds of mammalian antibodies.200111587642
[clinical and microbiological study of adult periodontal disease].the aim of this study was to carry out a microbiological evaluation of sites with and without clinical evidence of moderate and severe periodontitis and their correlation with clinical parameters. a total of 52 disease sites and 10 healthy sites were selected according to clinical criteria. the following clinical indexes were measured for all the sites: plaque index, gingival index, blood on probing, depth on probing and insertion level. samples of subgingival plaque were collected for culture a ...200111594003
roles of native topology and chain-length scaling in protein folding: a simulation study with a go-like model.we perform folding simulations on 18 small proteins with using a simple go-like protein model and analyze the folding rate constants, characteristics of the transition state ensemble, and those of the denatured states in terms of native topology and chain length. near the folding transition temperature, the folding rate k(f) scales as k(f) approximately exp(-c rco n(0.6)) where rco and n are the relative contact order and number of residues, respectively. here the topology rco dependence of the ...200111601854
comparative in vitro activity of ertapenem and 11 other antimicrobial agents against aerobic and anaerobic pathogens isolated from skin and soft tissue animal and human bite wound infections.we studied the comparative in vitro activity of ertapenem, a new carbapenem, against 240 aerobic and 180 anaerobic recent clinical bite isolates using an agar dilution method and an inoculum of 10(4) cfu/spot for aerobes and 10(5) cfu/spot for anaerobes. ertapenem inhibited 410/420 (98%) of the isolates tested at < or = 4 mg/l with only 4/5 campylobacter gracilis and 1/3 campylobacter rectus strains requiring . or = 16 mg/l for inhibition. ertapenem was only moderately active (mic 8 mg/l) agains ...200111679553
efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters.the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition.200111686813
absence of a specific subgingival microflora in adults with down's syndrome.periodontal disease in down's syndrome (ds) is generally characterized by a high degree of bone loss. bone loss of 5 mm or more is observed in 70% of these subjects. among ds subjects, considerable differences in disease progression occur. so far, no studies have been conducted in which specific properties of the subgingival microflora have been related to the condition observed.200111686820
azotobacter vinelandii ferredoxin i: a sequence and structure comparison approach to alteration of [4fe-4s]2+/+ reduction potential.the reduction potential (e(0)') of the [4fe-4s](2+/+) cluster of azotobacter vinelandii ferredoxin i (avfdi) and related ferredoxins is approximately 200 mv more negative than the corresponding clusters of peptostreptococcus asaccharolyticus ferredoxin and related ferredoxins. previous studies have shown that these differences in e(0)' do not result from the presence or absence of negatively charged surface residues or in differences in the types of hydrophobic residues found close to the [4fe-4 ...200211704670
single-site mutations induce 3d domain swapping in the b1 domain of protein l from peptostreptococcus magnus.thermodynamic and kinetic studies of the protein l b1 domain (ppl) suggest a folding pathway in which, during the folding transition, the first beta hairpin is formed while the second beta hairpin and the alpha helix are largely unstructured. the same mutations in the two beta turns have opposite effects on the folding and unfolding rates. three of the four residues composing the second beta turn in ppl have consecutive positive phi angles, indicating strain in the second beta turn.200111709166
[antimicrobial activities of carbapenems and fourth generation cephems against clinically isolated strains].we determined the antibacterial activities of 4 carbapenems (imipenem, panipenem, meropenem and biapenem) and 2 forth generation cephems (cefozopran and cefepime) against 850 bacterial strains (18 species) isolated during the period from january 1998 through september 2000. the 4 carbapenems showed excellent antibacterial activities against methicillin-susceptible s. aureus (mssa), s. pneumoniae, e. faecalis, p. aeruginosa, m. (b). catarrhalis, b. fragilis and peptostreptococcus spp. as compared ...200111729712
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.200111737506
supragingival and subgingival microbiota of adult patients with down's syndrome. changes after periodontal this longitudinal study, five adult down's syndrome patients with periodontitis were placed on a frequent recall visit schedule (every 6 weeks) after treatment, in order to investigate: 1) the microbiological status, both supragingivally and subgingivally, and the changes that occurred after treatment and 2) the effect of frequent professional supragingival plaque control on the subgingival microbiota and clinical variables in these patients. the clinical variables recorded were probing pocke ...200111737662
splenic abscess caused by peptostreptococcus species, diagnosed with the aid of abdominal computerized tomography and treated with percutaneous drainage and antibiotics: a case report.we describe herein a 63-year-old patient with a splenic abscess due to peptostreptococcus spp., diagnosed with the aid of abdominal computerised tomography and treated with ultra-sound guided percutaneous drainage and antibiotics. the bacteriological characteristics of splenic abscesses are discussed.200111744179
structure, specificity, and mode of interaction for bacterial albumin-binding modules.we have determined the solution structure of an albumin binding domain of protein g, a surface protein of group c and g streptococci. we find that it folds into a left handed three-helix bundle similar to the albumin binding domain of protein pab from peptostreptococcus magnus. the two domains share 59% sequence identity, are thermally very stable, and bind to the same site on human serum albumin. the albumin binding site, the first determined for this structural motif known as the ga module, co ...200211751858
[use of the etest method for antimicrobial susceptibility testing of obligate anaerobes].the aim of this study was to evaluate etest usefulness for antimicrobial susceptibility testing of obligate anaerobes and to compare the activity of five antibacterial drugs against clinical strains of anaerobes. one hundred strains of obligate anaerobes were tested: 2 reference strains (b. fragilis atcc 25285 and b. thetaiotaomicron atcc 29741) and 98 clinical strains isolated from patients of the infant jesus clinical hospital--center for trauma treatment in warsaw during the last three years ...200111757426
aggressive periodontitis associated with fanconi's anemia. a case report.fanconi's anemia is an autosomal recessive disease associated with chromosomal breakage as well as pancytopenia, skin pigmentation, renal hypoplasia, cardiac defects, microcephaly, congenital malformations of the skeleton, hypogonadism, and increased risk of leukemia. the present report describes the periodontal clinical and microbiological status of an 11-year old male having fanconi's anemia.200111759873
the bacterial flora in inflammatory bowel disease: current insights in pathogenesis and the influence of antibiotics and probiotics.the pathogenesis of inflammatory bowel disease (ibd) remains unknown, although in recent years more data have become available. the contribution of genetic and environmental factors is evident, and the luminal bacterial flora plays a major role in the initiation and perpetuation of chronic ibd. animal models of ibd have shown that colitis does not occur in a germ-free environment. in human ibd, inflammation is present in parts of the gut containing the highest bacterial concentrations. moreover, ...200111768558
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1998 and march 1999].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in japan since july 1982. this paper describes the results obtained in fiscal 1998 (from april 1998 to march 1999). the number of cases investigated as objectives was 225 for one year. a total of 429 strains (121 strains from primary infections and 308 strains from postoperative infections) were isolated from 183 cases (81.3% of total cases). in primar ...200111771334
ertapenem: a new carbapenem.ertapenem is a new 1-beta-methyl carbapenem, stable to dehydropeptidase, which binds preferable to penicillin-binding proteins (pbp) 2 and 3. ertapenem has a broad antibacterial spectrum with mic90 values < 0.5 mg/l for penicillin-susceptible streptococcus pneumoniae, streptococcus pyogenes, methicillin-sensitive staphylococcus aureus, haemophilus influenzae, moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., serratia spp., proteus spp., clostridium perfringens, fusobact ...200111772242
accurate computer-based design of a new backbone conformation in the second turn of protein l.the rational design of loops and turns is a key step towards creating proteins with new functions. we used a computational design procedure to create new backbone conformations in the second turn of protein l. the protein data bank was searched for alternative turn conformations, and sequences optimal for these turns in the context of protein l were identified using a monte carlo search procedure and an energy function that favors close packing. two variants containing 12 and 14 mutations were f ...200211786026
suppurative parotitis caused by anaerobic bacteria in newborns.staphylococci are the usual bacterial etiology of suppurative parotitis in newborns. this report describes for the first time recovery of anaerobic bacteria from aspirates of the infected gland in two infants with suppurative parotitis. peptostreptococcus intermedius and prevotella melaninogenica were isolated from one child and prevotella intermedia from the other patient. complete recovery occurred after 4 weeks of antimicrobial therapy.200211791111
the microbiology of evaluate the aerobic and anaerobic microbiology of mucopyocele.200111801943
periapical lesion progression with controlled microbial inoculation in a type i diabetic mouse model.uncontrolled or poorly controlled diabetes mellitus may be a risk factor for the development of large and/or debilitating periapical infections. the objectives of this investigation were to: (i) determine the effect of diabetes mellitus on the pathogenesis of periapical lesions with or without specific bacterial inoculations at the exposure sites, and (ii) test the sensitivities of two microbiological techniques in detecting the persistence of the bacterial inoculum in exposed pulps of nonobese ...200211806653
[a comparative study of various evaluation methods of the antibiotic sensitivity of strict anaerobic bacteria of the subgingival flora].the study on the sensitiveness of slow-growing anaerobes bacteria to antibiotics is delicate when you consider the technical motives that make it difficult to transpose the standard methods frequently used in microbiological laboratories. the three main methods used to determine susceptibility to antibiotics are: disk-diffusion test, antibiotics containing microdilution plates and atb ana (biomérieux). the aim of this study is to compare the effectiveness of each of these methods on severe anaer ...200111808376
[anaerobic infection and its rapid detection in surgical patients].to investigate anaerobic infection and its rapid detection in surgical patients.199911829950
in vitro antagonistic effect of lactobacillus on organisms associated with bacterial assess antagonistic properties of lactobacillus strains isolated from the vaginas of healthy women as compared to the most common bacterial agents related to vaginosis.200211838310
antimicrobial effects of 2.94 microm er:yag laser radiation on root surfaces: an in vitro study.this in vitro study investigated the antimicrobial effects of 2.94 microm er:yag laser radiation on root surfaces.200211846852
identification of predominant human and animal anaerobic intestinal bacterial species by terminal restriction fragment patterns (trfps): a rapid, pcr-based method.identification of predominant human and animal intestinal tract anaerobes by conventional methods is cumbersome, time-consuming and less sensitive as compared to molecular methods. we have developed a molecular technique to identify most of the abundant intestinal microflora by polyermase chain reaction (pcr) amplification of a 16s rrna gene fragment using a pair of universal pcr primers. the forward pcr primer was labelled with 6-carboxyfluorescein amino hexy (6-fam) fluorescent dye to detect t ...200111851378
[retrospective analysis of fusobacterium associated infections; experience at aomori prefectural hospital with 108 cases from 1995 to 1999].we experienced 108 cases of fusobacterium associated infections, including otolaryngeal, oral, pleuropulmonary, intraabdominal, skin and soft tissue infections, at aomori prefectural hospital during the 5 year-period from 1995 to 1999. a total of 433 organisms, included 113 fusobacterium spp. (80 fusobacterium nucleatum, 18 fusobaterium necrophorum, 5 fusobacterium varium, 4 fusobacterium mortiferum, 6 fusobacterium spp.), were recovered with an average of 4.0 organisms per case of the 108 cases ...200211852471
effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions.the aim of this study was to evaluate the fate of microorganisms in root canals of teeth with infected pulps and periapical bone lesions with and without the use of calcium hydroxide medication.200211858203
in vitro activity of gemifloxacin compared to seven other oral antimicrobial agents against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis.using a microbroth method for aerobes and agar dilution for anaerobes, we studied the comparative in vitro activity of gemifloxacin, three fluoroquinolones, two macrolides and two beta-lactams, against 207 aerobic and 162 anaerobic antral sinus puncture isolates. gemifloxacin was active at < or = 0.25 ug/ml against 198/207 [96%] aerobes and 127/162 [78%] anaerobes or 325/369 [88%] of all isolates and was the most active quinolone on a weight basis against gram-positive organisms. all haemophilus ...200211858906
antimicrobial activity of sch 27899, oligosaccharide member of the everninomycin class with a wide gram-positive spectrum.the in vitro antimicrobial activity of sch 27899 (everninomycin), a novel oligosaccharide compound of the everninomycin class, was compared with vancomycin, chloramphenicol, clinafloxacin, teicoplanin and doxycycline against 428 clinical strains of bacteria. everninomycin base exhibited the greatest antimicrobial activity compared to other formulations against all strains tested (mic90: 0.25 microg/ml) followed by clinafloxacin and teicoplanin (mic90: 0.5 microg/ml), vancomycin (mic90: 2 microg/ ...199511866719
[the microflora of ulzerous zone mucosa in patients with duodenal ulcer].bacteriological study of the biopsies taken from gastric and duodenal mucosa of 10 healthy volunteers and 74 patients with duodenal ulcer, was carried out. in the gastroduodenal zone of healthy subjects microorganisms of 6 genera (streptococcus, candida, staphylococcus, bacillus, helicobacter and lactobacillus) were detected. h. pylori was isolated in 20% of cases only in biopsy specimens taken from the antral section of the stomach of healthy as monoculture or in combination with c. albicans. i ...200111871291
bacteriocin-like activity of butyrivibrio fibrisolvens jl5 and its effect on other ruminal bacteria and ammonia production.when ruminal bacteria from a cow fed hay were serially diluted into an anaerobic medium that had only peptides and amino acids as energy sources, little growth or ammonia production was detected at dilutions greater than 10(-6). the 10(-8) and 10(-9) dilutions contained bacteria that fermented carbohydrates, and some of these bacteria inhibited clostridium sticklandii sr, an obligate amino acid-fermenting bacterium. phylogenetic analysis indicated that the most active isolate (jl5) was closely r ...200211872448
periodontal bacteria in rabbit mandibular and maxillary abscesses.despite the high incidence of odontogenic abscesses in pet rabbits, published data on the bacteriology of these infections are lacking, and clinical cultures are often ambiguous, making antibiotic choices difficult. in order to define the bacteriology of these infections, 12 rabbit mandibular and maxillary abscesses were cultured aerobically and anaerobically. all specimens yielded pathogenic bacteria, including fusobacterium nucleatum, prevotella heparinolytica, prevotella spp., peptostreptococ ...200211880435
differences in backbone dynamics of two homologous bacterial albumin-binding modules: implications for binding specificity and bacterial adaptation.proteins g and pab are bacterial albumin-binding proteins expressed at the surface of group c and g streptococci and peptostreptococcus magnus, respectively. repeated albumin-binding domains, known as ga modules, are found in both proteins. the third ga module of protein g from the group g streptococcal strain g148 (g148-ga3) and the second ga module of protein pab from p.magnus strain alb8 (alb8-ga) exhibit 59% sequence identity and both fold to form three-helix bundle structures that are very ...200211884146
maternal periodontitis and prematurity. part ii: maternal infection and fetal exposure.clinical data from the first 812 deliveries from a cohort study of pregnant mothers entitled oral conditions and pregnancy (ocap) demonstrate that both antepartum maternal periodontal disease and incidence/progression of periodontal disease are associated with preterm birth and growth restriction after adjusting for traditional obstetric risk factors. in the current study we present measures of maternal periodontal infection using whole chromosomal dna probes to identify 15 periodontal organisms ...200111887461
subgingival microbiota of indigenous indians of central define the subgingival microbial profiles of adult subjects from a previously identified rural community of indigenous indians in guatemala, central america.200211895544
meningitis and shunt infection caused by anaerobic bacteria in children.this review describes the microbiology and management of meningitis and shunt infections caused by anaerobic bacteria in children. the predominant anaerobes recovered in meningitis are bacteriodes spp., bacteriodes fragilis, fusobacterium spp., and clostridium spp. peptostreptococcus, veillonella, actinomyces, propionibacterium acnes, and eubacterium are less commonly isolated. the predisposing conditions for meningitis are acute or chronic middle-ear infection, sinusitis, pharyngitis, and pulmo ...200211897473
bacterial meningoencephalomyelitis in dogs: a retrospective study of 23 cases (1990-1999).the clinical records of 23 dogs (1990-1999) with histopathologically confirmed bacterial meningoencephalomyelitis were evaluated retrospectively. no breed, age, sex, or weight predisposition was found. all the dogs presented with clinical signs of a brain lesion, whereas 5 of 23 had neck pain. pyrexia was detected in 11 of 23 dogs on admission. cbcs revealed neutrophilic leucocytosis in 7 of 21 dogs and thrombocytopenia in 3 of 21 dogs. the serum chemistry profiles were abnormal in 15 of 21 dogs ...200211899031
[four cases of non-clostridial gas gangrene with diabetes mellitus].we report four cases of non-clostridial gas gangrene. all cases were associated with diabetes mellitus as the underlying disease. case 1: a 60-year-old male developed an ulcerative lesion on the dorsum of his left foot. peptostreptococcus asaccharolyticus, citrobacter freundii and staphyrococcus epidermidis were identified in culture from odoriferous pus. case 2: a 81-year-old female developed a lesion on her vulva spreading to the right lower abdomen. bacteroides bivius, peptostreptococcus asac ...200211915237
acute suppurative thyroiditis caused by an infected piriform sinus fistula with thyrotoxicosis.we report herein an unusual case of thyrotoxicosis caused by acute suppurative thyroiditis (ast) infected through a piriform sinus fistula (psf). a 28-year-old man presented with pain over the thyroid gland and elevated serum thyroid hormone levels, a picture similar to subacute thyroiditis. a fine-needle aspiration biopsy from the left lobe showed neutrophil infiltration, and culture from the aspirate grew anaerobic peptostreptococcus. a neck computed tomography (ct) scan showed an abscess in t ...200211916288
management of acute suppurative parotitis. 200211928106
bacteriology and antimicrobial susceptibility of gram-positive cocci isolated from pus specimens of orofacial odontogenic infections.we recently reported the beta-lactamase production and antimicrobial susceptibility of anaerobic gram-negative rods isolated from pus specimens of 93 orofacial odontogenic infections. in this report, we determine the bacteriology and antimicrobial susceptibility of bacteria other than anaerobic gram-negative rods, mainly gram-positive cocci, isolated from the same specimens. streptococcus constellatus and peptostreptococcus micros were frequent isolates from all types of infection examined. pept ...200211929563
treatment of papillon-lefèvre syndrome periodontitis.conventional mechanical treatment of papillon-lefèvre syndrome periodontitis has a poor prognosis. this report describes an effective antimicrobial treatment of rapidly progressing periodontitis in an 11-year old girl having papillon-lefèvre syndrome.200211966936
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--i. gram-positive bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, and carbapenems. changes in the bacterial sensitivity for czop were also evaluated with the resistance ratio calculated with breakpoint mic. sixteen species (1,913 strains) of gram-positive bacteria were isolated from the clinical materials annually collected fr ...200211977919
quantitative microbiological study of human carious dentine by culture and real-time pcr: association of anaerobes with histopathological changes in chronic pulpitis.the bacteria found in carious dentine were correlated with the tissue response of the dental pulps of 65 teeth extracted from patients with advanced caries and pulpitis. standardized homogenates of carious dentine were plated onto selective and nonselective media under anaerobic and microaerophilic conditions. in addition, real-time pcr was used to quantify the recovery of anaerobic bacteria. primers and fluorogenic probes were designed to detect the total anaerobic microbial load, the genera pr ...200211980945
bacteriology of acute and chronic frontal sinusitis.aspirates of 15 acutely and 13 chronically infected frontal sinuses were processed for aerobic and anaerobic bacteria. a total of 20 isolates (1.3 per specimen) were recovered from the 15 cases of acute frontal sinusitis, 16 aerobic and facultative isolates (1.1 per specimen) and 4 anaerobic isolates (0.3 per specimen). aerobic and facultative organisms alone were recovered in 13 specimens (87%), and mixed aerobic and anaerobic bacteria were recovered in 2 (13%). the predominant aerobic and facu ...200212003592
in vitro bactericidal activity and post-antibiotic effect of abt-773 versus co-amoxiclav against anaerobes. 200212003989
the effects of an immediately pre-surgical chlorhexidine oral rinse on the bacterial contaminants of bone debris collected during dental implant implant surgery produces bone debris that can be used in the "simultaneous augmentation" technique. although this debris is contaminated with oral bacteria, a stringent aspiration protocol has been shown to reduce the levels of contamination. chlorhexidine mouthrinse is a well-proven antibacterial rinse that has been shown to reduce infectious complications associated with dental implants. this study examined the effect of pre-operative rinsing with a 0.1% chlorhexidine digluconate mouthr ...200212005141
parotid salivary s-iga antibodies during experimental gingivitis in smokers and non-smokers.persons who smoke display a less pronounced increase of gingival bleeding in the experimental gingivitis model as compared with non-smokers. the aim of the present study was to investigate whether this could partly be explained by differences in levels of parotid total secretory iga (s-iga) or parotid s-iga reactive with selected oral microorganisms. parotid saliva samples were obtained from 11 smoking and 14 non-smoking volunteers, at baseline, after 5 and 14 days of full mouth experimental gin ...200212009188
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