PMID(sorted ascending)
management of an infected hematometrocolpos in a patient with congenital adrenal hyperplasia and vaginal stenosis. 19989679917
in vitro and in vivo antibacterial activities of am-1155 in the fields of obstetrics and is a new 8-methoxyquinolonecarboxylic acid with a broad spectrum of antibacterial activity. it inhibited more than 90% of clinical isolates of streptococcus agalactiae, escherichia coli, peptostreptococcus magnus, bacteroides fragilis and prevotella bivia at the concentration of 3.13 mg/l. the antibacterial activity of am-1155 was almost equal to that of sparfloxacin. the in vivo efficacy of am-1155 was evaluated using a polymicrobial pyometra (e. coli and b. fragilis) model of rats. the ...19989681200
experimental root canal infections in conventional and germ-free mice.a small animal model was evaluated to study the interrelationships between microorganisms after their implantation in root canals (inferior central incisors) using germ-free (gf) and conventional (cv) mice. the selected microorganisms were: porphyromonas endodontalis (atcc 35406), eubacterium lentum (atcc 25559), peptostreptococcus anaerobius (atcc 27337), fusobacterium nucleatum (atcc 10953), escherichia coli (atcc 25922), and enterococcus faecalis (atcc 4083). only p. anaerobius, e. coli, and ...19989693583
female genital tract bacterial coisolates with candida albicans in patients without clinical vitro, candida albicans has demonstrated the ability to inhibit replication of selected bacteria. little information exists on the impact of c. albicans on the vaginal bacterial flora in vivo. the purpose of this study is to identify the coexisting bacterial flora when c. albicans is isolated from vaginal cultures submitted to a hospital-based testing facility for reasons other than vulvovaginitis.19989702585
ovarian abscess caused by peptostreptococcus magnus following transvaginal ultrasound-guided aspiration of ovarian endometrioma and fixation with pure ethanol.transvaginal ultrasound-guided aspiration of ovarian endometrioma has been applied and emphasized as a safe and simple procedure.19989702588
[a case report of epidural abscess due to anaerobic bacteria, producing a mass of gas].a 54-year-old female was admitted to our hospital on may 8, 1996, for evaluation of fever and headache. her cerebrospinal fluid showed elevation of the cell count and total protein. the thoracic and lumbar mris revealed abnormal gas occupying the epidural space at the sacral level, and extended as high as 9th thoracic level. the gas spread inside the iliopsoas and paraspinal muscles. anaerobic bacteria such as bacteroides fragilis and peptostreptococcus productus were cultured from the spinal fl ...19989711118
superficial suppurative thrombophlebitis in children, caused by anaerobic bacteria.the purpose of this study was to present the microbiology and clinical features of six children with superficial suppurative thrombophlebitis (sst) caused by anaerobic bacteria.19989722004
subgingival microbial profile of papillon-lefèvre patients assessed by dna-probes.the prevalence of 18 selected bacterial species was assessed by means of "checkerboard" dna-dna hybridisation in a group of 12 saudi-arabian adolescents with papillon-lefèvre syndrome. a total of 36 tooth sites were investigated. the patients exhibited severe periodontal disease with deep pockets. all 12 patients harboured the putative bacterial pathogens p. intermedia, f. nucleatum, p. micros and s. intermedius while t. denticola, b. forsythus, p. nigrescens, e. corrodens, s. noxia and c. rectu ...19989722266
oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis.the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ...19989722273
preterm birth: associations with genital and possibly oral microflora.opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ...19989722706
in vitro activities of e1101, a novel oral cephalosporin, against bacteria causing infections in obstetric and gynecological patients.e1101 is a new oral cephalosporin with a broad spectrum of antibacterial activity. it inhibited more than 90% of clinical isolates of streptococcus agalactiae, escherichia coli and peptostreptococcus magnus at the concentration of 3.13 mg/l. e1101 was the most active agent against s. agalactiae and e. coli. since none of the compounds was sufficiently active against the bacteroides fragilis and prevotella bivia isolates, they are not appropriate in the treatment of patients with infections cause ...19989732148
in-vitro antibiotic susceptibility and molecular analysis of anaerobic bacteria isolated in cape town, south hundred and twenty-three anaerobic isolates from cape town, south africa, were tested in vitro against cefoxitin, chloramphenicol, metronidazole, co-amoxiclav, benzylpenicillin and clindamycin. forty-five gram-positive organisms were tested against rp59500 (a streptogramin). resistance in the gram-positive isolates was as follows: of the clostridium perfringens isolates, 4% were resistant to benzylpenicillin and 4% to clindamycin; of the peptostreptococcus anaerobius isolates, 10% were resis ...19989738844
increased antibody production against gut-colonizing escherichia coli in the presence of the anaerobic bacterium peptostreptococcus.germ-free rats were colonized with e. coli alone, or with e. coli plus lactobacillus acidophilus and a strain of the obligate anaerobic gram-positive species, peptostreptococcus. the presence of peptostreptococcus reduced translocation of e. coli, but increased the serum antibody response to e. coli antigen. whereas the immunoglobulin g (igg) anti-e. coli antibodies largely represented cross-reactive antibodies, those of the immunoglobulin m (igm) isotype represented true anti-e. coli antibodies ...19989743213
[bacterial flora in chronic purulent maxillary sinusitis].in the years from 1993 till 1995 there were 132 tests done on occurrence aerobes (oxygen bacteria) and 56 tests on occurrence of anaerobes and fungus in patients with chronic sinusitis. the most common microorganisms among the aerobes was haemophilus influenzae (23.1%) and staphylococcus aureus (20.9%). the most common microorganisms among the anaerobes was peptococcus and peptostreptococcus (together 57.1%) and from the strains bacteroides (36.8). the breeded oxygen microorganisms gr(+) were mo ...19979757687
phenotypic and phylogenetic characterization of ruminal tannin-tolerant bacteria.the 16s rrna sequences and selected phenotypic characteristics were determined for six recently isolated bacteria that can tolerate high levels of hydrolyzable and condensed tannins. bacteria were isolated from the ruminal contents of animals in different geographic locations, including sardinian sheep (ovis aries), honduran and colombian goats (capra hircus), white-tail deer (odocoileus virginianus) from upstate new york, and rocky mountain elk (cervus elaphus nelsoni) from oregon. nearly compl ...19989758806
[peptostreptococcus magnus osteoarticular infections after orthopedic surgery. 14 cases and pathogenicity factors].we report a retrospective study of 14 peptostreptococcus magnus bone and joint infections, following orthopaedic prostheses or implantation of fixation devices, diagnosed in two paris hospitals between 1992 and 1996. five patients experienced a knee joint infection after anterior cruciate ligament reconstruction with 4 artificial grafts, and 9 caught joint or wound infections, after limb traumatic injuries or bone neoplastic ruptures involving femur, tibia, calcaneum and humerus, treated by arth ...19989769879
[in vitro activity of 10 antibiotics including pristinamycin and its two components (rp 12536 and 27404) against strict anaerobes].the activities of the pristinamycin and its two components (rp 12536 and rp 27404) were investigated using the reference agar dilution method m11a3, on 175 anaerobic strains in comparison with that of erythromycin, clindamycin, metronidazole, amoxycillin either alone or combined with clavulanic acid, piperacillin, cefoxitin, cefotetan and cefotaxime. beta-lactamase production was detected for all the 55 b. fragilis group strains and 8/12 prevotella and 2/18 fusobacterium strains, respectively. o ...19989769928
sinusitis in neurologically impaired children.the microbiologic features of infected sinus aspirates in nine children with neurologic impairment were studied. anaerobic bacteria, always mixed with aerobic and facultative bacteria, were isolated in 6 (67%) aspirates and aerobic bacteria only in 3 (33%). there were 24 bacterial isolates, 12 aerobic or facultative and 12 anaerobic. the predominant aerobic isolates were klebsiella pneumoniae, escherichia coli, and staphylococcus aureus (2 each) and proteus mirabilis, pseudomonas aeruginosa, hae ...19989781990
aerobic and anaerobic microbiology of infection after trauma.clinical and laboratory data from 1973 to 1988 were retrospectively reviewed to study the microbiology of infection following trauma. a total of 368 specimens obtained from 340 trauma patients showed bacterial growth. the traumas included lacerations (163), blunt trauma (76), penetrating trauma (65), bites (20), and open fractures (10). anaerobic bacteria only were isolated in 119 (32%) specimens, aerobic bacteria only in 58 (16%), and mixed aerobic-anaerobic flora in 191 (52%). a total of 444 a ...19989786544
limited internal friction in the rate-limiting step of a two-state protein folding reaction.small, single-domain proteins typically fold via a compact transition-state ensemble in a process well fitted by a simple, two-state model. to characterize the rate-limiting conformational changes that underlie two-state folding, we have investigated experimentally the effects of changing solvent viscosity on the refolding of the igg binding domain of protein l. in conjunction with numerical simulations, our results indicate that the rate-limiting conformational changes of the folding of this do ...19989811844
microorganisms in closed periapical lesions.the purpose of this study was to investigate the microorganisms of strictly selected closed periapical lesions associated with both refractory endodontic therapy and pulpal calcification. definitive criteria were established that assured complete clinical isolation of the periapical lesion from the oral and periodontal environment. a total of 13 criteria-referenced lesions were selected from 70 patients with endodontic surgical indications. a well controlled culturing method was used in all case ...19989823127
recovery of anaerobic bacteria from a post-traumatic nasal septal abscess. a report of two cases.this report presents 2 children (boys, 11 and 9 years old) who had post-traumatic nasal septal abscesses. cultures for aerobic and anaerobic bacteria revealed peptostreptococcuus magnus and a beta-lactamase-producing prevotella intermedia in the first patient, and peptostreptococcus anaerobius, streptococcus intermedius, and prevotella melaninogenica in the second patient. anaerobic bacteria may play a role in nasal septal abscesses.19989823846
survey of anaerobic susceptibility patterns: a french multicentre 1996, the in vitro antibiotic susceptibility of 463 anaerobes was measured in five hospitals using the reference agar dilution method. none of the 209 b. fragilis group strains showed resistance to imipenem or ticarcillin-clavulanic acid. high resistance rates (29%) were observed for cefotetan and clindamycin. beta-lactamase production was detected respectively in 64% of the prevotella and 7% of the fusobacterium strains. because the same standardized methods were used for many years, the aut ...19989832284
additional clinical and microbiological effects of amoxicillin and metronidazole after initial periodontal therapy.the aims of this study were to evaluate the clinical and microbiological effects of initial periodontal therapy (it) and to determine the additional effects of systemic amoxicillin (flemoxin solutab) 375 mg tid plus metronidazole 250 mg tid therapy, in patients with adult actinobacillus actinomycetemcomitans (aa)-associated periodontitis in conjunction with either porphyromonas gingivalis (pg), bacteroides forsythus (bf) and/or prevotella intermedia (pi). in addition the adverse effects of the a ...19989846793
proficiencies of three anaerobic culture systems for recovering periodontal pathogenic bacteria.anaerobic culture is employed routinely in the primary isolation of periodontal pathogenic bacteria. however, little or no data exist on the relative abilities of the coy anaerobic chamber (coy laboratory products, grass lake, mich.), the gaspak (becton dickinson microbiology systems, cockeysville, md.), and the anaeropack (mitsubishi gas chemical america, inc., new york, n.y.) systems to grow important periodontal species, including porphyromonas gingivalis, prevotella intermedia/nigrescens, ba ...19999854085
microbiology of liver and spleen study the aerobic and anaerobic microbiology of liver and spleen abscesses and correlate the results with predisposing factors, potential causes and routes of infection, clinical and laboratory data of 48 patients with liver abscesses and 29 with spleen abscesses treated between 1970 and 1990 were reviewed retrospectively. in liver abscesses, a total of 116 isolates (2.4 isolates/specimen) was obtained; 43 were aerobic and facultative species (0.9 isolates/specimen) and 73 were anaerobic spec ...19989856643
[bacterial flora in pharyngitis and tonsillitis].the aim of the study was a microbiological analysis of pharyngeal swabs obtained from 158 patients with the diagnosis of pharyngitis and purulent exudates from the tonsillar crypts of 10 patients treated for chronic purulent tonsillitis. beta haemolytic streptococci groups a, b, c and g were isolated from 30% of the patients. the most frequently isolated were streptococcus pyogenes--12% of patients and streptococcus group c--10.7%. other streptococci were isolated less frequently: streptococcus ...19989857615
microbiologic characteristics of persistent otitis identify the pathogens isolated from children with acute otitis media who did not respond to antimicrobial drug therapy.19989865757
[a case of subacute bacterial endocarditis caused by anaerobic peptostreptococcus]. 19979868074
[a study of anaerobic infection in maxillofacial region].to analyse the anaerobic infection of maxillofacial surgery and estimate the efficacy of antianaerobic therapy, 45 patients were divided into two groups, tinidazole group and metronidazole group. bacterial culture was positive before treating in all cases. there were bacillus melaniogenicus, veillonella, peptococcus and peptostreptococus, etc. there was excellent efficacy in the treatment of maxillofacial anaerobic infection by tinidazole intravenously. after treatment, the result of bacterial e ...19979868121
protein la, a novel hybrid protein with unique single-chain fv antibody- and fab-binding properties.existing ig-binding proteins all suffer from limitations in their binding spectrum. in the pursuit of the ultimate, non-restricted, ig-binding protein, we have constructed the hybrid protein la, by fusing four of the ig kappa light-chain-binding domains of peptostreptococcal protein l with four of the iggfc- and fab-binding regions of staphylococcal protein a. ligand-blot experiments demonstrated that the l and the a components were both functional in the hybrid, as the protein was shown to bind ...19989874260
oropharynx microbiota among alcoholics and non-alcoholics.the oropharynx microbiota plays an important role in the origin of infections, especially among alcoholics whose airway defenses are impaired.19989876451
trichomonas tenax empyema in an immunocompromised patient with advanced cancer.a 53-year-old male acromegalic patient with advanced rectal adenocarcinoma developed pleuritis in the course of cobalt irradiation, steroid treatment and chemotherapy. examination of drained pleural fluid demonstrated numerous motile organisms, which were identified as trichomonas tenax by giemsa staining. peptostreptococcus micros was also detected in the cultures of pleural fluid and blood. treatment with metronidazole successfully eliminated the protozoa and cured the pyothorax.19989879561
a commensal symbiosis between prevotella bivia and peptostreptococcus anaerobius involves amino acids: potential significance to the pathogenesis of bacterial both batch and continuous culture, peptostreptococcus anaerobius was able to grow in vaginal defined medium with prevotella bivia, but not in pure culture. growth of p. anaerobius was increased by 238% (p < 0.001) in peptone-supplemented vaginal defined medium conditioned by prior growth of p. bivia. analysis of p. bivia culture supernatants showed a net accumulation of amino acids and subsequent growth of p. anaerobius in the conditioned supernatants resulted generally in amino acid utilizat ...19989879923
pathogenicity of peptostreptococcus micros morphotypes and prevotella species in pure and mixed culture.recently, an atypical rough colony morphotype of peptostreptococcus micros, a species which is found in ulcerating infections, including periodontitis, was isolated. the virulence of morphotypes alone and in combination with prevotella intermedia and p. nigrescens was investigated both in vivo and in vitro. all strains tested induced abscesses containing fluid pus in a mouse skin model, and lesions caused by monocultures of the rough morphotype strains of p. micros were statistically significant ...19989879956
[clinical evaluation of lung abscess diagnosed by transtracheal aspiration].we have diagnosed lung abscess according to findings of infiltration with cavity formation on chest x-ray and/or ct-scan and pathogens isolated from transtracheal aspirates. we evaluated the clinical features of 20 patients with lung abscess (18 males and 2 females, mean age; 54.3 years). diabetus mellitus and periodontal diseases were prominent underlying diseases in patients with lung abscess. cough was complained in 13 patients, chest or back pain in 9, purulent sputum in 8 and hemosputum in ...19989884505
microbiology of perianal cellulitis in children: comparison of skin swabs and needle establish the aerobic and anaerobic microbiology of perianal cellulitis in children, comparing skin swab and needle aspirate methodology.19989888333
spondylitis caused by peptostreptococcus.peptostreptococcus is an anaerobic gram-positive coccus that is encountered in dental and sinus infections, pelvic infections in women, osteomyelitis, arthritis, skin and soft-tissues infections, conjunctivitis and bacteraemia [1,2]. we report here, what is to our knowledge, the first cases of spondylitis with discitis caused by peptostreptococcus.19989890688
comparative anti-anaerobic activity of men 10700, a penem antibiotic.the in vitro activity of men 10700, a new penem, has been compared with that of metronidazole, clindamycin, ciprofloxacin, co-amoxiclav, imipenem and three third generation cephalosporins against 120 strains of anaerobes. the organisms tested comprised clostridium perfringens, clostridium difficile, bacteroides fragilis and speciated members of the genera fusobacterium, veillonella and peptostreptococcus. men 10700 showed activity similar to that of imipenem, and was more potent than metronidazo ...19989916909
aerobic and anaerobic microbiology of axillary hidradenitis suppurativa.a retrospective review of the microbiological and clinical data of 17 specimens obtained from axillary hidradenitis suppurativa (hs) over a period of 6 years was undertaken to study the aerobic and anaerobic microbiology of this condition. a total of 42 bacterial isolates (2.5 per specimen) were obtained, 12 aerobic or facultative (0.7 per specimen) and 30 anaerobic or micro-aerophilic (1.8 per specimen). aerobic and facultative bacteria only were isolated in six (35%) cases, anaerobic bacteria ...19999920133
purification and properties of nadh-dependent 5, 10-methylenetetrahydrofolate reductase (metf) from escherichia coli.a k-12 strain of escherichia coli that overproduces methylenetetrahydrofolate reductase (metf) has been constructed, and the enzyme has been purified to apparent homogeneity. a plasmid specifying metf with six histidine residues added to the c terminus has been used to purify histidine-tagged metf to homogeneity in a single step by affinity chromatography on nickel-agarose, yielding a preparation with specific activity comparable to that of the unmodified enzyme. the native protein comprises fou ...19999922232
coronal leakage: endotoxin penetration from mixed bacterial communities through obturated, post-prepared root canals.coronal leakage has been considered as one of the major causes of failure in root canal treatment. bacteria have been shown to penetrate the root canal obturating materials and influence the periapical tissues. endotoxin, a component of gram-negative bacteria, is a potent inflammatory agent and may be able to penetrate obturating materials faster than bacteria. this in vitro study examined the possible penetration of post-prepared canals by endotoxin and bacterial cells derived from mixed bacter ...19989922745
[bilateral tubo-ovarian actinomycosis and rheumatoid arthritis]. 19989974016
aerobic and anaerobic microbiology of surgical-site infection following spinal fusion.the aerobic and anaerobic microbiology of surgical-site infections (ssi) following spinal fusion was retrospectively studied. this was done by reviewing the clinical and microbiological records at the naval hospital in bethesda, md., from 1980 to 1992. aspirates of pus from 25 infection sites showed bacterial growth. aerobic bacteria only were recovered from 9 (36%) specimens, anaerobic bacteria only were recovered from 4 (16%), and mixed aerobic and anaerobic bacteria were recovered from 12 (48 ...19999986872
isolation of fusobacterium necrophorum from cancrum oris (noma).a study of the predominant microflora in active sites of noma (cancrum oris) lesions was carried out in eight noma patients 3-15 years of age in sokoto state in northwestern nigeria. paper point sampling and conventional anaerobic microbiologic techniques were used. fusobacterium necrophorum was recovered from 87.5% of the noma lesions. oral microorganisms included prevotella intermedia, alpha-hemolytic streptococci, and actinomyces spp. which were isolated from 75.0%, 50.0%, and 37.5% of the pa ...19999988340
elastase activity of anaerobes isolated from amniotic fluid with preterm premature rupture of membranes.a total of 131 anaerobes isolated from amniotic fluid with preterm premature rupture of membranes and stored were examined for elastolytic activity by the method described by williams et al (lett appl microbiol 1988;7:173-6).19999988804
comparative in vitro activities of amoxicillin-clavulanate against aerobic and anaerobic bacteria isolated from antral puncture specimens from patients with an agar dilution method, the antimicrobial susceptibilities of antral sinus puncture isolates were studied. pneumococci were generally susceptible to amoxicillin, azithromycin, and clarithromycin, but 17% of pneumococcal isolates were resistant to cefuroxime. haemophilus influenzae isolates were resistant to amoxicillin and clarithromycin. beta-lactamase production occurred in 69% of prevotella species. one-third of peptostreptococcus magnus isolates were resistant to azithromycin and clarith ...199910049296
relationship of the subgingival microbiota to a chairside test for aspartate aminotransferase in gingival crevicular fluid.the aim of the present study was to evaluate the association between the occurrence of certain specific periodontal pathogens and aspartate aminotransferase (ast) levels in gingival crevicular fluid (gcf).199910052771
infections associated with dental procedures in total hip procedures may lead to a transient bacteraemia lasting for up to 30 minutes. of the numerous cases of total hip arthroplasty (tha) reported which have been infected from haematogenous sources, dental procedures have been involved only infrequently. we reviewed the records of 2973 patients after tha. of the late infections identified in 52 patients, three (6%) were strongly associated with a dental procedure. infection was diagnosed by culture from the affected joint; streptococcus viridan ...199910068004
infection after rotator cuff repair.sixteen patients (15 men and 1 woman) were treated for infection complicating rotator cuff repair during the period 1975 through 1994. eight of the 16 patients had their initial procedure performed elsewhere. the remaining 8 procedures were performed at our institution with the known incidence of this complication being 0.27%. in addition to intravenous antibiotic therapy, an average of 3.5 (range 2 to 8) operative procedures were required to eradicate the infections. micro-organisms cultured we ...199910077787
clinical and microbiological characteristics of smokers with early onset periodontitis.cigarette smoking is a potential risk factor which has recently been associated with periodontal disease progression. the objective of this study was to compare the microbial profile of smokers and non-smokers in a group of patients with early onset periodontitis. the study population consisted of 60 healthy individuals, 40 males and 20 females aged 22 to 35 yr, exhibiting early onset periodontitis. thirty patients were smokers (30.9 cigarettes/d) and 30 non-smokers. smokers had a higher proport ...199910086883
the microbiota of periodontal pockets with different depths in therapy-resistant periodontitis.this study presents the composition of the cultivable microbiota colonising periodontal pockets of different depths among 2 patient-groups classified as non-responsive (nr-group; 11 participants) or responsive (r-group; 10 participants) to periodontal treatment. microbiological samples from three types of pocket (< 4 mm deep a-samples; 4-5 mm b-samples; > 5 mm c-samples) were analysed by cultural methods for putative periodontitis pathogens, microbial groups constituting > or = 5% of the total c ...199910100039
microbiota of successful osseointegrated dental implants.the long-term survival of dental implants depends, in part, on control of bacterial infection in the peri-implant region. periodontal pathogens colonized implants symptomatic through infection, whereas the microbiota of successful implants was similar to that of periodontal health. this study examined the impact on the peri-implant microbiota of crown restorations; implant type; length of time of loading; history of implant or periodontal infections; and whether implants replaced single or multi ...199910102550
in vitro antibacterial properties of pexiganan, an analog of magainin.pexiganan, a 22-amino-acid antimicrobial peptide, is an analog of the magainin peptides isolated from the skin of the african clawed frog. pexiganan exhibited in vitro broad-spectrum antibacterial activity when it was tested against 3,109 clinical isolates of gram-positive and gram-negative, anaerobic and aerobic bacteria. the pexiganan mic at which 90% of isolates are inhibited (mic90) was 32 micrograms/ml or less for staphylococcus spp., streptococcus spp., enterococcus faecium, corynebacteriu ...199910103181
comparative activity of eighteen antimicrobial agents against anaerobic bacteria isolated in south africa.the in vitro activity of 18 antimicrobial agents was determined against 378 anaerobic bacteria isolated in bloemfontein, south africa, during 1996/97. against the gram-positive isolates, mics of penicillin and cefoxitin were >0.5 microg/ml and >16 microg/ml, respectively, for five and three strains of non-perfringens clostridium spp. seventeen peptostreptococcus anaerobius strains were resistant to penicillin (mic > or = 2 microg/ml). all gram-positive anaerobes tested except one peptostreptococ ...199910192714
intra-operative ultrasound-guided drainage of parotid abscess.parotitis complicated by parotid abscess remains a potentially life-threatening problem. conventional surgical treatment involves incising the parotid parenchyma in the direction of the facial nerve until the abscess is located and evacuated. intra-operative ultrasound greatly assists in localizing the abscess and in ensuring its complete drainage. expeditious and exact localization of the abscess reduces operative time. equally importantly, ultrasound-assisted drainage reduces surgical dissecti ...199810197156
cloning of fiba, encoding an immunogenic subunit of the fibril-like surface structure of peptostreptococcus micros.although we are currently unaware of its biological function, the fibril-like surface structure is a prominent characteristic of the rough (rg) genotype of the gram-positive periodontal pathogen peptostreptococcus micros. the smooth (sm) type of this species as well as the smooth variant of the rg type (rgsm) lack these structures on their surface. a fibril-specific serum, as determined by immunogold electron microscopy, was obtained through adsorption of a rabbit anti-rg type serum with excess ...199910198013
coaggregation of candida dubliniensis with fusobacterium nucleatum.the binding of microorganisms to each other and oral surfaces contributes to the progression of microbial infections in the oral cavity. candida dubliniensis, a newly characterized species, has been identified in human immunodeficiency virus-seropositive patients and other immunocompromised individuals. c. dubliniensis phenotypically resembles candida albicans in many respects yet can be identified and differentiated as a unique candida species by phenotypic and genetic profiles. the purpose of ...199910203506
adherence of peptostreptococcus micros morphotypes to epithelial cells in vitro.peptostreptococcus micros, which is associated with oral and non-oral mixed anaerobic infections, occurs in three colony morphotypes, the smooth type, the rough type and the smooth variant of the rough type. these types differ in surface structures; the rough type expresses large fibrillar surface appendages, which are absent on the surface of both the smooth and the smooth variant of the rough type. to determine the role of these surface structures in adherence we characterized the adherence of ...199910204480
coagulase-negative staphylococci are the most common bacteria found in cultures from the deep portions of hidradenitis suppurativa lesions, as obtained by carbon dioxide laser surgery.the significance of bacterial findings in hidradenitis suppurativa (hs) is controversial. interpretation of the results of bacteriological examinations from the surface of hs lesions is obscured by the possible contamination of resident skin bacteria. bacteriological analysis of aspirates from deeper parts of hs is liable to show low sensitivity. we used a carbon dioxide (co2) laser method to evaporate the diseased tissue level by level from the surface downwards, allowing concurrent sampling of ...199910215774
metronidazole susceptibility testing of anaerobic bacteria associated with periodontal disease.the aim of the present investigation was to determine the susceptibility of prevotella intermedia, porphyromonas gingivalis, fusobacterium and peptostreptococcus micros to metronidazole in vitro. two methods were applied on each isolated strain: agar dilution and epsilometer etest. a total of fifty three wild test strains (13 p.intermedia, 14 p. gingivalis, 14 f.spp and 12 p.micros) were isolated from patients with periodontitis. the etest appears to be a simple, rapid and reliable method for th ...199910223399
in vitro activities of pazufloxacin, a novel injectable quinolone, against bacteria causing infections in obstetric and gynecological patients.t-3762 is an injectable new quinolone with a broad spectrum of antibacterial activity. pazufloxacin (t-3761) is an active form of t-3762. the minimal inhibitory concentrations (mics) of pazufloxacin for 50% of the clinical isolates tested were 3.13 microg/ml for streptococcus agalactiae, 6.25 microg/ml for gardnerella vaginalis, 0.025 microg/ml for escherichia coli, 0.78 microg/ml for pseudomonas aeruginosa, 6.25 microg/ml for peptostreptococcus magnus, 6.25 microg/ml for bacteroides fragilis an ...199910224336
interactions between a single immunoglobulin-binding domain of protein l from peptostreptococcus magnus and a human kappa light chain.the placement of a tryptophan residue into a single ig-binding-domain of protein l from peptostreptococcus magnus has been used to examine the binding interactions between the binding domain and kappa light chains (kappa-chains). the fluorescence intensity of the mutant domain increases on the formation of a complex with kappa-chains. this has been used to determine the kd of the complex under a range of conditions by using both pre-equilibrium and equilibrium methods. the kd values determined f ...199910229674
bacterial populations contaminating the upper gut in patients with small intestinal bacterial overgrowth syndrome.small intestinal bacterial overgrowth syndrome (sibos) is characterized by an abnormally high bacterial population level in the upper gut, exceeding 10(5) organisms/ml (5 log colony-forming unit (cfu)/ml). to understand its origin and select an appropriate antibiotic treatment, we have analyzed the bacterial populations contaminating the upper gut in sibos patients.199910235214
the family coriobacteriaceae: reclassification of eubacterium exiguum (poco et al. 1996) and peptostreptococcus heliotrinreducens (lanigan 1976) as slackia exigua gen. nov., comb. nov. and slackia heliotrinireducens gen. nov., comb. nov., and eubacterium lentum (prevot 1938) as eggerthella lenta gen. nov., comb. nov.16s rrna gene sequences were determined for eubacterium exiguum and peptostreptococcus heliotrinreducens. these species were found to be closely related and, together with eubacterium lentum, to constitute a branch of the coriobacteriaceae. two new genera are proposed on the basis of phenotypic characteristics and 16s rrna gene sequence comparisons: slackia to include the bile-sensitive species eubacterium exiguum and p. heliotrinreducens, and eggerthella to include the bile-resistant eubacteriu ...199910319481
microbiological and clinical effects of a 1% chlorhexidine-gel in untreated periodontal pockets from adult periodontitis patients.the aim of this study was to report the microbiological and clinical effects of repeated subgingival administration of a 1% chlorhexidine-gel in periodontal pockets from 10 patients with adult periodontitis. results showed that the experimental treatment significantly improved clinical parameters (plaque index, gingival bleeding index, and pocket probing depth). direct subgingival administration of chlorhexidine-gel also produced a remarkable modification in the proportions of putative periodont ...199910322610
prevalence of corynebacterial 16s rrna sequences in patients with bacterial and "nonbacterial" prostatitis.the etiology of chronic prostatitis syndromes in men is controversial, particularly when positive cultures for established uropathogens are lacking. although identification of bacteria in prostatic fluid has relied on cultivation and microscopy, most microorganisms in the environment, including some human pathogens, are resistant to cultivation. we report here on an rrna-based molecular phylogenetic approach to the identification of bacteria in prostate fluid from prostatitis patients. positive ...199910325338
interference by aerobic and anaerobic bacteria in children with recurrent group a beta-hemolytic streptococcal compare the frequency of recovery of aerobic and anaerobic bacteria with interfering capability of group a beta-hemolytic streptococci (gabhs) in the tonsils of children with and without a history of recurrent gabhs pharyngotonsillitis.199910326813
comparative microbiological characteristics of failing implants and periodontally diseased teeth.the purpose of this report was to compare the distribution of periodontal pathogens recovered from failing implants and teeth with adult and recurrent forms of periodontitis.199910328655
fungi in therapy-resistant apical periodontitis.the occurrence of yeasts in 967 microbiological endodontic samples taken from root canals in persistent endodontic infections was studied. the sampling was done by general practitioners in various parts of finland from root canal infections which did not respond favourably to standard conservative therapy. the samples were cultivated aerobically on a non-selective enriched horse blood agar medium, on tsbv agar medium in 5% co2 and anaerobically on horse blood agar medium. micro-organisms were fo ...199710332243
differential induction of colitis and gastritis in hla-b27 transgenic rats selectively colonized with bacteroides vulgatus or escherichia coli.resident bacteria play an important role in initiating and perpetuating gastrointestinal inflammation. we previously demonstrated that six commensal bacteria including bacteroides vulgatus caused more aggressive colitis and gastritis in hla-b27 transgenic rats than did the other five bacteria without b. vulgatus. this study compared the degree of gastrointestinal inflammation in gnotobiotic hla-b27 transgenic rats monoassociated with either b. vulgatus or escherichia coli. gnotobiotic transgenic ...199910338507
effects of hydrogen peroxide on growth and selected properties of porphyromonas this study we first evaluated the effects of hydrogen peroxide (h2o2) on growth and selected properties of porphyromonas gingivalis, and compared them with those obtained by a reducing agent (cysteine). the growth of p. gingivalis was only moderately affected when h2o2 was added at concentrations up to 30 mm in a complex culture medium. however, when a defined basal medium was used, h2o2 at a concentration of 3 mm completely inhibited growth of p. gingivalis. incorporation of cysteine at conc ...199910339828
[multiple abscesses (cerebral, splenic, cutaneous) and pulmonary infection]. 199910365514
antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis the present study, the antibiotic susceptibility of most prevalent micro-organisms in advanced periodontitis patients was evaluated. in 56 patients, pooled subgingival plaque samples were taken from the deepest site of each quadrant and were cultivated anaerobically. from each patient, the 4 most frequently encountered types of bacterial colonies were subcultured and identified (rapid id 32 a). from all bacterial species identified in the 224 subcultures, the 4 most prevalent were used for su ...199910382573
flow cytometry to monitor phagocytosis and oxidative burst of anaerobic periodontopathogenic bacteria by human polymorphonuclear leukocytes.the reduced susceptibility to phagocytosis found among some periodontopathogenic anaerobes may account for the differences between invasive and non-invasive strains. we applied flow cytometry as a powerful tool to analyze and quantify phagocytosis using standardized cultures of oral anaerobes (porphyromonas gingivalis, prevotella intermedia, p. nigrescens, capnocytophaga gingivalis, c. ochracea, c. sputigena, fusobacterium nucleatum and peptostreptococcus micros) and heparinized whole blood. bac ...199910384401
inflammatory mediators in saliva of patients with rapidly progressive periodontitis during war stress induced incidence increase.rapidly progressive periodontitis (rpp) results from the interaction between the periodontal microflora and the host. stress is believed to play an important role in determining host responses, and it has been proposed that hyperactivity of host defense mechanisms significantly increases tissue destruction typical for this disease. during a period of four months we have diagnosed 20 patients with acute rpp, all of them active participants in battles of the croatian liberation war with posttrauma ...199910402713
microbiology of retroperitoneal abscesses in children.samples of pus from 41 children with retroperitoneal abscess treated between 1974 and 1994 yielded a total of 125 organisms (3.0 isolates/specimen); 58 isolates were aerobic and facultative species (1.4/specimen) and 67 were anaerobic (1.6/specimen). aerobic bacteria only were isolated from 7 (17%) abscesses, anaerobic bacteria only from 3 (7%) and mixed aerobic and anaerobic bacteria from 31 (76%); 34 (83%) infections were polymicrobial. the predominant aerobic and facultative isolates were esc ...199910403422
destructive knee joint infection caused by peptostreptococcus micros: importance of early microbiological diagnosis.peptostreptococcus micros is a commensal of the oral cavity and the genitourinary tract that rarely causes serious infections. a case of a destructive knee joint infection with rapid progress caused by p. micros is presented. the significance of the microbiological findings was initially not acknowledged, which contributed to a nonsuccessful clinical outcome.199910405436
clinical and microbiological effects of initial periodontal therapy in conjunction with amoxicillin and clavulanic acid in patients with adult periodontitis. a randomised double-blind, placebo-controlled study.the aim of the present study was to investigate the clinical and microbiological effects of initial periodontal therapy in conjunction with systemic amoxicillin plus clavulanic acid in adult periodontitis patients using a double-blind, parallel-group, and placebo-controlled protocol. 21 patients with a clinical diagnosis of generalised adult periodontitis were recruited. clinical measurements and microbiological assessments were carried out at baseline, 3, and 12 months post-treatment. approxima ...199910412851
bacteremia after dental treatment in mentally handicapped people.bacteremia may occur after disruption of the oral mucous membrane, particularly after dental treatment. 18 mentally handicapped patients who underwent dental treatment with general anesthesia were included in our study. none of the patients had general illnesses or received antibiotic protection. from each patient several blood samples were drawn aseptically during dental treatment and cultured. the majority of aerobic bacteria recovered belonged to streptococcus sp and gemella sp., anaerobic ba ...199910412852
porphyromonas gingivalis prevalence related to other micro-organisms in adult refractory periodontitis.forty-six adult periodontal patients, selected on the basis of clinical examination, and 46 adult healthy subjects were examined. the subgingival plaque samples from one inflammatory and one non-inflammatory site of each periodontal patient were studied to determine porphyromonas gingivalis prevalence related to other periodontal micro-organisms and to periodontal tissue destruction. the results showed porphyromonas gingivalis as the main pathogenic micro-organism isolated in the inflammatory si ...199910423739
periodontitis associated bacteria in supragingival plaque of dental hygienists: stability of carrier state and clinical development.the purpose of this study was the clinical and microbiological re-examination of dental hygienists, who, 30 months before, had shown remarkably high supragingival levels of periodontitis-associated micro-organisms. interdental plaque was collected from the same molar sites and investigated by the same immunofluorescence assay with taxa-specific monoclonal antibodies as at the initial examination. on average, the 15 re-examined subjects showed slightly increased plaque levels but unchanged bleedi ...199910424388
activity of telithromycin (hmr 3647) against anaerobic bacteria compared to those of eight other agents by time-kill methodology.time-kill studies examined the activities of telithromycin (hmr 3647), erythromycin a, azithromycin, clarithromycin, roxithromycin, clindamycin, pristinamycin, amoxicillin-clavulanate, and metronidazole against 11 gram-positive and gram-negative anaerobic bacteria. time-kill studies were carried out with the addition of oxyrase in order to prevent the introduction of co(2). macrolide-azalide-ketolide mics were 0.004 to 32.0 microg/ml. of the latter group, telithromycin had the lowest mics, espec ...199910428930
[trovafloxacin in gynecology].presence of microorganisms in the female lower genital tract (lgt) in concentrations that modify the established normal equilibrium produce different symptoms that make necessary to consult the gynecologist. it is currently accepted that infections of the lgt are due to microorganisms that are normally integrating the internal flora, except for the erroneously called "sexually transmitted diseases" that are caused by external microorganisms. this means that all those microorganisms usually prese ...199910436555
cervical necrotizing fasciitis: 10 years' experience at a single evaluate clinical and microbiologic characteristics, modalities of treatment and outcome of patients with cervical necrotizing fasciitis admitted to our institution.199910447540
infection-stimulated infraosseus inflammation and bone destruction is increased in p-/e-selectin knockout mice.infections of the dental pulp commonly result in infraosseus inflammation and bone destruction. however, the role of phagocytic leucocytes in the pathogenesis of pulpal infections has been uncertain. in this work we used p/e-/- selectin-deficient mice, which lack rolling adhesion of leucocytes to endothelium and mimic the human syndrome, leucocyte adhesion deficiency ii (lad-ii), to test the hypothesis that phagocytic leucocytes protect against pulpal infection and subsequent periapical infraoss ...199910447722
in vitro activity of gemifloxacin (sb 265805) against anaerobes.gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...199910471570
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents: special references to bacteria isolated between july 1996 and june 1997].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in 20 facilities in japan since july 1982. this paper describes the results obtained during period from july 1996 to june 1997. the number of cases investigated as objectives was 217 for one year. a total of 406 strains were isolated from 177 cases (81.6% of total cases). from primary infections 162 strains were isolated, and from postoperative infecti ...199910480049
[in vitro bactericidal activities of new oral penem, faropenem against the various clinical isolates].the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of faropenem (frpm) more compared with those of various oral beta-lactams against 15 isolates each of 6 species of microorganism. frpm possessed potent in vitro antibacterial activity against both aerobic and anaerobic gram-positive bacteria tested. frpm showed the same activity as new oral cephems such as cefdinir, cefditoren and cefcapene against all gram-negative bacteria, but k. pneumoniae strains were le ...199910480050
[clinical evaluation of anaerobic infections in patients with bronchopulmonary infections diagnosed by transtracheal aspiration].we evaluated the clinical and bacteriologic features in the patients with bronchopulmonary infections isolated anaerobes from transtracheal aspirates between april 1990 and march 1998. some anaerobe was isolated in 42 (10.9%) in 387 patients whom we performed transtracheal aspiration (tta), in 42 (15.7%) of 268 in whom some organism was isolated from tta, or in 42 (16.3%) of 257 patients in whom some bacterium excluding acid-fast bacteria, fungi or mycoplasma from tta. the isolation rate of anae ...199910481403
microbiological features of papillon-lefèvre syndrome periodontitis.papillon-lefevre syndrome patients exhibit hyperkeratosis palmo-plantaris and severe periodontitis. the syndrome is an autosomal recessive trait, but the mechanism of periodontal destruction is not known. this report presents the clinical and microbiological features of an 11-year old girl with papillon-lefèvre syndrome. clinical examination included conventional periodontal measurements and radiographic analysis. in samples from 3 deep periodontal lesions, the occurrence of major suspected peri ...199910487314
the microbiology of infected and noninfected leg ulcers.a clinical study was undertaken to investigate and compare specifically the aerobic and anaerobic microbiology of infected and noninfected leg ulcers.199910487444
microbiology of infected pustular psoriasis lesions.bacterial infections can occur in lesions of pustular psoriasis (pp). the objective of this study was to establish the aerobic and anaerobic microbiology of secondarily infected pp.199910487445
recurrent acute suppurative thyroiditis in a child: case report.a 23-month-old girl presented with a history of persistent fever and growing left anterior neck mass following an upper respiratory tract infection. laboratory studies revealed leukocytosis, elevated levels of the erythrocyte sedimentation rate and c-reactive protein, and a mild impairment of thyroid function. thyroid scan showed a decreased radioactive iodine uptake of the left thyroid gland. culture of the thyroid aspirate grew the mixed flora, viridans streptococci, prevotella spp, and peptos ...199810496157
acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification.perinatally, and between menarche and menopause, increased levels of estrogen cause large amounts of glycogen to be deposited in the vaginal epithelium. during these times, the anaerobic metabolism of the glycogen, by the epithelial cells themselves and/or by vaginal flora, causes the vagina to become acidic (ph approximately 4). this study was designed to test whether the characteristics of acid production by vaginal flora in vitro can account for vaginal acidity. eight vaginal lactobacillus is ...199910496892
tetracycline-coated polytetrafluoroethylene barrier membranes in the treatment of intraosseous periodontal lesions.periodontal pathogens are detrimental to periodontal healing in barrier membrane-assisted periodontal therapy. tetracycline-coating of barrier membranes may reduce levels of infecting pathogens. this study evaluated the clinical and microbiological effects of tetracycline-coated expanded polytetrafluoroethylene (t-eptfe) barrier membranes in the treatment of 2- to 3-wall intraosseous periodontal lesions around mandibular molars.199910505803
microbiological understandings and mysteries of noma (cancrum oris).the microbiologic history of noma was reviewed. studies have associated the disease process with large numbers of fusiform bacilli and spirochetal organisms. in order to study the microbiology of the staging and infection periods of noma 62 nigerian children, aged 3-14 years, 22 children had acute necrotizing ulcerative gingivitis (anug) and were also malnourished, 20 exhibited no acute necrotizing ulcerative gingivitis but were malnourished and 20 were free of acute necrotizing ulcerative gingi ...199910522213
in vitro susceptibility to pexiganan of bacteria isolated from infected diabetic foot ulcers.during two clinical trials involving the treatment of 835 outpatients with infected diabetic foot ulcers, 2515 bacterial isolates, including 2337 aerobes and 178 anaerobes, were grown from cultures of the ulcers. the in vitro susceptibility of these isolates was determined to pexiganan, a peptide anti-infective evaluated in these clinical trials, and to other classes of antibiotics. pexiganan demonstrated broad spectrum antimicrobial activity against gram-positive and gram-negative aerobes and a ...199910529881
beta-hemolytic streptococci and other beta-hemolytic organisms in apical periodontitis and severe marginal periodontitis.thirteen teeth with necrotic pulps and apical periodontitis and nine severe periodontal pockets were cultured for presence of beta-hemolytic streptococci and other beta-hemolytic organisms. samples were dispersed and plated on two non-selective and one selective growth media and incubated anaerobically and in 10% co2 in air. a total of 59 beta-hemolytic colonies were purified and identified. eight beta-hemolytic streptococcal isolates were obtained from three of the severe marginal periodontitis ...199910530151
dexamethasone differentially regulates cytokine transcription and translation in macrophages responding to bacteria or okadaic acid.many microorganisms and microbial products induce expression of pro-inflammatory cytokines such as interleukin-1 (il-1alpha/beta) and tumour necrosis factor-alpha (tnf-alpha) in macrophages, primarily by transcriptional activation. we show here, by using mouse macrophages in primary culture, that pre-treatment with dexamethasone inhibits bacteria-induced il-1beta expression as mrna and cellular pro-il-1beta in parallel, consistent with an effect primarily on transcriptional activation. in contra ...199910530874
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