PMID(sorted ascending)
treatment of peptostreptococcus micros endocarditis with teicoplanin. 19958562764
a case of anaerobic pericarditis. 19958562770
microbiology of study the microbiologic and clinical characteristics of patients with mediastinitis.19968572845
identification and discrimination of oral asaccharolytic eubacterium spp. by pyrolysis mass spectrometry and artificial neural networks.curie-point pyrolysis mass spectra were obtained from 29 oral asaccharolytic eubacterium strains and 6 abscess isolates previously identified as peptostreptococcus heliotrinreducens. pyrolysis mass spectrometry (pyms) with cluster analysis was able to clarify the taxonomic position of this group of organisms. artificial neural networks (anns) were then trained by supervised learning (with the back-propagation algorithm) to recognize the strains from their pyrolysis mass spectra; all eubacterium ...19968574131
endodontic pathogens in periodontal disease augmentation.periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endodontic infection. the aim ...19958583015
oral microbiota in subjects with a weak or strong response in experimental gingivitis.the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ...19958583022
phylogenetic analysis of ruminococcus flavefaciens, the type species of the genus ruminococcus, does not support the reclassification of streptococcus hansenii and peptostreptococcus productus as ruminococci.the 16s rrna gene sequence of the type strain of ruminococcus flavefaciens, the type species of the genus ruminococcus, was determined by pcr direct sequencing. a comparative sequence analysis showed that r. flavefaciens is phylogenetically related to a small cluster (cluster iv of collins et al. [m. d. collins, p. a. lawson, a. willems, j. j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 44:812-826, 1994]) of organisms which include ...19958590686
antimicrobial and toxic effects of established and potential root canal irrigants.three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants peridex, chlorhexidine gluconate (0.12%), and therasol were tested. alcohol and saline served as controls also. the in vitro portion of this study used four different microorganisms (streptococcus mutans, peptostreptococcus micros, prevotella intermedius, and porphyromonas gingivalis) to determine antimicrobial effect. presterilized 7-mm diameter whatman paper disks soaked with t ...19958596073
pet findings in a brain abscess associated with a silent atrial septal defect.brain abscesses are classical complications of congenital heart disease (chd) in children and adolescents. this association is rarely observed in adults. we report a 46-year-old man presenting a fronto-parietal abscess associated with an asymptomatic atrial septal defect. positron emission tomography (pet) study revealed high uptake of l-[methyl-11c]methionine ([11c]methionine) and 2-[18f]fluoro-2-deoxy-d-glucose (fdg) around the brain abscess. we suggest (1) to exclude a silent cardiac malforma ...19958599907
bacteriology and beta-lactamase activity in acute and chronic maxillary assess the bacteriology and beta-lactamase enzyme activity in aspirates of 10 acutely and 13 chronically inflamed maxillary sinuses.19968600928
microbiological features and pathogenesis of peritonsillar abscesses.samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ...19958605900
aerobic and anaerobic microbiology of infected study the aerobic and anaerobic microbiology of infected hemorrhoids.19968607502
endodontic pathogens: propagation of infection through patent dentinal tubules in traumatized monkey teeth.periapical pathology indicating endodontic infection, when present in marginal periodontitis-affected teeth, has recently been shown to be an aggravating factor in progression of marginal destruction. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endo ...19958625937
clinical significance of dental root canal microflora.previous work by this group has shown that a significant association exists between pain and the presence of either prevotella or peptostreptococcus spp. in dental root canals. the aim of this study was to examine a more extensive series of canals microbiologically, to determine whether any other particular endodontic symptoms or clinical signs showed specific associations with individual bacterial species.19968636492
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
backbone dynamics of a domain of protein l which binds to immunoglobulin light chains.protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule interacts with the variable domain of immunoglobulin (ig) light chains through five repeated homologous domains denoted b1 to b5. the fold of the ig-light-chain-binding b1 domain of protein l (plb1) has been shown to comprise an alpha-helix packed against a four-stranded beta-sheet and therefore resembles the structure of the igg-binding domains o ...19968654399
effect of co2 on the fermentation capacities of the acetogen peptostreptococcus productus u-1.the fermentative capacities of the acetogenic bacterium peptostreptococcus productus u-1 (atcc 35244) were examined. although acetate was formed from all the substrates tested, additional products were produced in response to co2 limitation. under co2-limited conditions, fructose-dependent growth yielded high levels of lactate as a reduced end product; lactate was also produced under co2-enriched conditions when fructose concentrations were elevated. in the absence of supplemental co2, xylose-de ...19968655492
the in vitro activity of trospectomycin against anaerobic bacteria.the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...19958667035
isolation of non-sporing anaerobic rods from infections in children.from 1974 to 1994, 2033 microbiological specimens from children were submitted for cultures for anaerobic bacteria. fifty-seven isolates of bifidobacterium spp. were obtained from 55 (3%) children, 67 isolates of eubacterium spp. from 65 (3%) children and 41 isolates of lactobacillus spp. from 40 (2%) children. most bifidobacterium isolates were from chronic otitis media, abscesses, peritonitis, aspiration pneumonia and paronychia. most eubacterium isolates were from abscesses, peritonitis, decu ...19968667406
bacteremia shortly after placental separation during cesarean assess frequency, risk factors, and microbiology of bacteremia within 15 minutes of placental separation during cesarean delivery.19968677085
[computed tomographic demonstration of intraspinal air after an infection with peptostreptococci]. 19968679984
[diagnosis and treatment of local forms of surgical anaerobic non-clostridial infection].145 patients with such kind of surgical infection have been taken to clarify the cause of it. aerobic agents were the cause in 16.6% and anaerobic nonclostridial agents--in 33.1% of all cases. the mixed flora was the cause in 44.1%. the most important place among anaerobic nonclostridial infections belongs to bacteroids (38.3%), peptostreptococcus (27.3%) and the peptococcus (17.2%). one must use wide-spectrum antibiotic for a treatment of a such kind of infection tiel the usults of microbiologi ...19968683929
pneumonia in mechanically ventilated children.the quantitative aerobic and anaerobic microbiology of bronchial aspirates, obtained using protective brush catheters, from 10 children with ventilator-associated pneumonia, is presented. aerobic or faculative organisms only were isolated in 1 child, anaerobic bacteria only in 3, and aerobic mixed with anaerobic bacteria in 6. there were 10 aerobic or faculative and 17 anaerobic isolates. the predominant aerobes were pseudomonas aeruginosa (2 isolates) and klebsiella sp. (2). the predominant ana ...19958685643
fetal fibronectin and microorganisms in vaginal fluid of healthy pregnant determine fetal fibronectin in vaginal fluid from healthy women during uncomplicated pregnancy and to investigate possible relationships to gestational age and the vaginal microflora.19968693926
[a case of the greater omentum abscess due to peptostreptococcus ssp].a case of the greater omentum abscess due to peptostreptococcus ssp. is reported. a 63-year-old male was admitted to heisei memorial hospital with a complaint of abdominal pain. an intraperitoneal mass measuring 6 cm in diameter was detected by ct and ultrasonogram. he was diagnosed as having an intraperitoneal abscess. after antibiotics therapy by cefotiam, laparotomy was performed and an abscess about 7 cm in diameter at the edge of the greater omentum was found. there was no evidence of perfo ...19968699102
microbiology of subperiosteal orbital abscess and associated maxillary sinusitis.aspirate of pus from eight subperiosteal orbital abscesses (spoas) and their corresponding infected sinuses were studied for aerobic and anaerobic bacteria. polymicrobial flora was found in all instances, and the number of isolates varied from two to five. anaerobes were recovered from all specimens. the predominant isolates were peptostreptococcus spp, prevotella spp, fusobacterium spp, staphylococcus aureus, and microaerophilic streptococci. concordance in the microbiological findings between ...19968699891
pyomyositis in children, caused by anaerobic bacteria.the author describes the microbiology and clinical features of six pyomyositis infections in children, which yielded anaerobic bacteria. anaerobic bacteria alone were recovered in four instances, and they are mixed with facultative bacteria in two. there were 15 bacterial isolates (13 anaerobic, 2 facultative). the bacteria were peptostreptococcus sp (5 isolates), bacteroides fragilis (3), clostridium sp (2), fuso-bacterium nucleatum (1), prevotella sp (1), bateroides sp (1), streptococcus pyoge ...19968708910
identification of interdomain sequences promoting the intronless evolution of a bacterial protein the evolution of eukaryotic genes, introns are believed to have played a major role in increasing the probability of favorable duplication events, chance recombinations, and exon shuffling resulting in functional hybrid proteins. as a rule, prokaryotic genes lack introns, and the examples of prokaryotic introns described do not seem to have contributed to gene evolution by exon shuffling. still, certain protein families in modern bacteria evolve rapidly by recombination of genes, duplication ...19968710897
anaerobic septic bursitis: case report and review. 19968722967
in vitro activity of the tricyclic beta-lactam gv104326.gv104326 is a novel tricyclic beta-lactam (a trinem or, formerly, tribactam). the in vitro activity of gv104326 was compared with those of cefuroxime, cefixime, amoxicillin, amoxicillin-clavulanic acid, cefpirome, and ciprofloxacin. gv104326 had in vitro activity generally similar to that of cefixime against members of the family enterobacteriaceae (mic at which 90% of the isolates are inhibited [mic90], < or = 2 micrograms/ml), with cefuroxime and amoxicillin-clavulanic acid being 8- to 32-fold ...19968723475
antibacterial activity of quinupristin/dalfopristin. rationale for clinical use.most gram-positive organisms are highly susceptible to the streptogramin, quinupristin/dalfopristin (rp 59500; synercid). minimum inhibitory concentrations for 90% of isolates (mic90) were < or = 1 mg/l for staphylococcus aureus, s. epidermidis, s. haemolyticus, streptococcus pneumoniae, s. pyogenes and listeria monocytogenes. importantly, quinupristin/dalfopristin shows similar activity against methicillin-susceptible and -resistant strains of s. aureus, and streptococci with benzylpenicillin ( ...19968724814
response to periodontal therapy in patients with high or low levels of p. gingivalis, p. intermedia, p. nigrescens and b. a previous study, subjects receiving either adjunctive tetracycline or augmentin showed, on average, more attachment level gain 10 months post-therapy than subjects receiving either ibuprofen or a placebo, although some subjects in each treatment group showed loss of attachment post-therapy. since differences in treatment response might have been due to differences in the subgingival microbiota, the response to different therapies in subjects with different pre-therapy subgingival microbiotas ...19968739165
utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care.the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ...19968739166
comparison of spiral gradient endpoint and agar dilution methods for susceptibility testing of anaerobic bacteria: a multilaboratory collaborative evaluation.a multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (sge) method for the determination of the antimicrobial susceptibilities of anaerobes and to evaluate the equivalence of the mics obtained by the sge method with those obtained by the reference agar dilution method of the national committee for clinical laboratory standards. the standard deviation of the mic obtained by the sge method for the five participating laboratories was +/- 0.26 of ...19968748295
crystallization and x-ray analysis of a single fab binding domain from protein l of peptostreptococcus magnus.protein l is a multidomain cell wall constituent of certain strains of peptostreptococcus magnus which binds to the variable domain of immunoglobulin kappa-light chains. a single immunoglobulin-binding domain of m(r) = 9000 from this protein has been isolated and crystallized. the crystals are of space group p4(2)2(1)2, with cell dimensions a = b = 66.9 a, c = 68.3 a, and diffract to at least 2.2 a resolution. the asymmetric unit of the crystal contains two molecules of the protein l domain, rel ...19958749861
bacteriological epidemiology and treatment of bacterial hundred symptomatic women with clinical bacterial vaginosis (bv) were enrolled in this study. the bacteriological epidemiology of bv and efficacy of oral or vaginal administration of antimicrobial agents for treatment of bv were investigated. the epidemiology of bv was investigated before antimicrobial therapy. fifty patients were treated with oral administration of metronidazole (mtn), 500 mg twice a day, and 50 patients with 2% clindamycin (cldm) phosphate in a vaginal cream, 5 g once a da ...19968751270
polymerase chain reaction-based detection of bacteria in determine if the presently used bacterial detection techniques provide accurate and complete profiles of microorganisms found in human semen.19968751749
[antibiotic sensitivity of anaerobic bacteria isolated during one year (1993)].the aim of this study was to determine the antimicrobian susceptibility of the anaerobe bacteria isolated from clinical samples in the authors' department in 1993.19968756216
[in vitro activity of a new fluoroquinolone, marbofloxacin (ro 09-1168) against strictly anaerobic bacteria and some bacteria from human fecal flora].as recommended by the eec, the in vitro activity of a veterinary quinolone has to be evaluated on human strains isolated from the gut and especially against anaerobes. thus, the mics for marbofloxacin were determined by the reference agar method (norma nccls m11 a3) using wilkins chalgren medium for the 124 anaerobic strains and mueller hinton agar for e. coli, proteus, enterococcus and lactobacillus. on the whole aerobes, the activity of marbofloxacin was equal or slightly greater to that of of ...19968758470
direct evidence for a two-state protein unfolding transition from hydrogen-deuterium exchange, mass spectrometry, and nmr.we use mass spectrometry in conjunction with hydrogen-deuterium exchange and nmr to characterize the conformational dynamics of the 62-residue igg binding domain of protein l under conditions in which the native state is marginally stable. mass spectra of protein l after short incubations in d2o reveal the presence of two distinct populations containing different numbers of protected protons. nmr experiments indicate that protons in the hydrophobic core are protected in one population, whereas a ...19968762137
cloning, expression and purification of ppl-1, a kappa-chain binding protein, based upon protein l from peptostreptococcus magnus.protein l is a multi-domain, cell wall constituent of certain strains of peptostreptococcus magnus, which binds to the variable domain of the l-chains of ig. a gene fragment which codes for a single ig-binding domain of protein l (ppl-1) was cloned into a modified pkk223-3 vector and over-expressed in e. coli jm103. a rapid protein purification protocol is described. in these studies, purified ppl-1 was immobilised on to an agarose gel and tested against an array of igs and ig fragments. it was ...19958767928
a risk-benefit assessment of teicoplanin in the treatment of infections.teicoplanin is a glycopeptide antibiotic whose activity is selectively oriented against gram-positive aerobic and anaerobic bacteria, including staphylococcus aureus, coagulase-negative staphylococci, clostridium difficile, peptostreptococcus spp. and corynebacterium jeikeium; such activity is affected by neither methicillin resistance nor beta-lactamase production. teicoplanin is not significantly absorbed from the gastrointestinal tract; consequently, it has to be administered intravenously (e ...19958785019
[in vitro activities of sulopenem, a new parenteral penem, against anaerobes].in vitro activities of sulopenem, a novel parenteral penem, was compared with those of imipenem, flomoxef, cefuzonam, cefoperazone and sulbactam/ampicillin against 66 reference strains (19 genera, 61 species) and 392 recent clinical isolates of anaerobic bacteria and fastidious aerobic bacteria. sulopenem had a very broad spectrum against anaerobic bacteria. in general, this compound was active against anaerobic reference strains with mics of < or = 0.78 micrograms/ml, while being the least acti ...19968786627
susceptibilities of bovine summer mastitis bacteria to antimicrobial agents.the susceptibility to 9 antimicrobial agents of 32 aerobic bacterial isolates and to 10 antimicrobial agents of 37 anaerobic bacterial isolates from 23 cases of bovine summer mastitis (16 actinomyces pyogenes isolates, 8 streptococcus dysgalactiae isolates, 3 s. uberis isolates, 3 s. acidominimus isolates, 2 streptococcus spp., 15 peptostreptococcus indolicus isolates, 10 fusobacterium necrophorum isolates, and 12 isolates of anaerobic gram-negative rods) was determined by the agar dilution meth ...19968787898
microbiology of acute purulent pericarditis. a 12-year experience in a military study the aerobic and anaerobic micro-biological and clinical characteristics in 15 cases of acute pericarditis treated over a 12-year period.19968790081
microbiology of periapical abscesses and associated maxillary sinusitis.aspirate of pus from 5 periapical abscesses of the upper jaw and their corresponding maxillary sinusitis were studied for aerobic and anaerobic bacteria. polymicrobial flora were found in all instances, where the number of isolates varied from 2 to 5. anaerobes were recovered from all specimens. the predominant isolates were prevotella sp., porphyromonas sp., fusobacterium nucleatum, and peptostreptococcus sp. concordance in the microbiological findings between periapical abscess and the maxilla ...19968794972
clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects.thirteen periodontally healthy subjects were monitored clinically for 6-12 months. clinical measurements at 6-weekly intervals included duplicate pd measurements, presence of plaque, redness, and bleeding on probing. baseline measurements consisted of 2 visits 1 wk apart. microbial samples were taken from 11 of the subjects who had completed at least 8 months of monitoring. levels of serum antibodies to 12 periodontal species were determined from 10 subjects. standard deviations of replicate pd ...19968814590
bacteriologic findings in tonsillitis and pericoronitis.bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ...19968816129
amplified expression and large-scale purification of protein l'.the gene fragment (ppl') encoding the functional unit of peptostreptococcus protein l was isolated using pcr and expressed in e. coli. as the gene fragment lacked its own promoter, the 5' pcr primer was designed to incorporate an nde1 restriction site (catatg) into the gene. this enabled the gene to be cloned in frame into an nde1 restriction site immediately downstream of a trp promoter. to prevent read through, a stop codon was introduced into the 3' primer. expression of ppl' was up to 27% to ...19968818265
a purgative action of barbaloin is induced by eubacterium sp. strain bar, a human intestinal anaerobe, capable of transforming barbaloin to aloe-emodin anthrone.orally administered barbaloin (100 mg/kg) did not induce any diarrhea in male wistar rats, in spite of severe diarrhea with sennoside b (40 mg/kg). also, in gnotobiote rats mono-associated with peptostreptococcus intermedius, a human intestinal anaerobe capable of reducing sennidins to rhein anthrone, barbaloin did not induce diarrhea; the faecal water content (71.9%) 8 h after the administration of barbaloin was not increased, compared with that (73.9%) just before the treatment. however, sever ...19968820926
changing anaerobic spectrum in suppurative lung disease: a case report.a spectrum of three different anaerobes were isolated from a debilitated patient with suppurative lung disease, within a two-year period. repeated isolation from three consecutive samples and symptomatic relief with metronidazole provide clinical evidence of anaerobic lung infection. this case emphasizes the importance of anaerobic culture in cases of protracted pulmonary suppurative disease.19968822645
[antimicrobial activities of tosufloxacin against recently obtained clinical isolates].to evaluate antimicrobial activities of tosufloxacin (tflx), minimum inhibitory concentrations (mics) of tflx and other drugs were determined against clinical isolates that were obtained in our laboratory from february of 1993 to january of 1994 (period i), and from february of 1995 to january of 1996 (period ii). the results are summarized as follows: 1. compared to mic90's reported by others for tflx in the 1980's, those obtained by us against beta-streptococci, streptococcus pneumoniae, pepto ...19968828073
the dynamics of microbial colonization of barrier membranes for guided tissue regeneration.the microbial colonization of expanded polytetrafluoroethylene membrane by putative periodontopathogens at 3 minutes of intraoral manipulation was determined in 42 patients with 42 mandibular posterior two- to three-wall defects. twenty patients exhibited no periodontal pockets of > or = 5 mm, other than the study site, and low levels of pathogens (group a). twenty-two patients revealed multiple periodontal pockets of 5 mm or more and numerous pathogens (group b). within the preceding 3 months o ...19968832481
[aerobic and anaerobic bacterial flora in acute abdominal illnesses].366 specinmens taken from patients suffering from acute abdominal illnesses who had undergone operations were examined. bacterial pathogens were isolated from 318 specimens. aerobic bacteria were isolated from 53.8% specimens. anaerobic bacteria were isolated from 12.6% specimens. mixed bacterial flora were isolated from 33.6% specimens. among aerobic bacteria e. coli and streptococcus faecalis were most often isolated. among anaerobic bacteria bacteroides fragilis, peptostreptococcus spp. and p ...19958833932
sodium-dependent succinate decarboxylation by a new anaerobic bacterium belonging to the genus anaerobic bacterium was isolated from a polluted sediment, with succinate and yeast extract as carbon and energy sources. the new strain was gram-positive, the cells were coccal shaped, the mol% g+g content of the genomic dna was 29, and the peptidoglycan was of the l-ornithine-d-glutamic acid type. comparative sequence analysis of the 16s rrna gene showed the new strain to belong to the genus peptostreptococcus. succinate, fumarate, pyruvate, 3-hydroxybutyrate and lysine supported growth. su ...19968836437
aerobic and anaerobic microbiology of necrotizing fasciitis in children.specimens obtained from eight children with necrotizing fasciitis (nf) were cultured for aerobic and anaerobic bacteria. a total of 21 isolates were recovered, 13 anaerobic and 8 aerobic or facultatives. the facultative organism streptococcus pyogenes was present alone in two (25%) instances, and mixed aerobic and anaerobic bacteria were isolated in six (75%). the predominant isolates were peptostreptococcus spp. (6 isolates, including 3 peptostreptococcus magnus). s. pyogenes (4), bacteroides f ...19968844744
infection of a total knee joint prosthesis by peptostreptococcus micros and propionibacterium acnes in an elderly ra patient: implant salvage with longterm antibiotics and needle aspiration/irrigation.we describe the favourable long-term outcome after late total knee joint prosthesis infection in an elderly ra patient. peptostreptococcus micros and propionibacterium acnes were cultured from the synovial fluid, and the finding of a coexistent abscess at the root of a wisdom tooth suggested a dental origin to the joint infection. long-term antibiotics in conjunction with aspiration/irrigation resulted in salvage of the implant and an excellent functional outcome.19968853177
characterization of the binding properties of protein lg, an immunoglobulin-binding hybrid protein.protein lg is a 50-kda hybrid molecule containing four ig-light-chain-binding domains from protein l of peptostreptococcus magnus and two igg-fe-binding repeats from streptococcal protein g. here we analyse the binding of protein lg to ig from several mammalian species. protein lg was shown to bind human igg of all subclasses and other ig classes that carry kappa chains. the binding to human igg was only marginally influenced by changes in temperature (4-37 degrees c) or salt concentration (0-1. ...19968856054
acute phase response in heifers with experimentally induced mastitis.ten pregnant heifers were inoculated in both hind udder quarters with a mixture of actinomyces pyogenes, fusobacterium necrophorum and peptostreptococcus indolicus. development of the experimental mastitis was monitored by sequential clinical and bacteriological examinations, and by blood acute phase protein profiles. sequential changes in plasma fibrinogen, serum haptoglobin, acid-soluble glyco-proteins and alpha 1-proteinase inhibitor activity were analysed and compared with both the clinical ...19968864931
deep neck infections. 19968870932
in vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.the antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the national committee for clinical laboratory standards-approved wadsworth agar dilution technique. the species tested included bacteroides fragilis (n = 91), other members of the b. fragilis group (n = 130), campylobacter gracilis (n = 15), other bacteroides spp. (n = 16), prevotella spp. (n = 49), porphyromonas spp. (n = 15), fusobacterium spp. (n = 62), bilophila wadsworthia (n = 24), sutterel ...19968878617
protein pab, an albumin-binding bacterial surface protein promoting growth and virulence.the anaerobic bacterium peptostreptococcus magnus is a human commensal and pathogen. previous work has shown that strains of p. magnus isolated from patients with gynecological disease (vaginosis) frequently express an immunoglobulin (ig) light chain-binding protein called protein l. here we report that strains isolated from localized suppurative infections bind human serum albumin (hsa), whereas commensal isolates bind neither ig nor hsa. the hsa-binding protein pab was extracted from the bacte ...19968900134
in vitro activity of quinupristin/dalfopristin (rp 59500) against a large collection of infrequently isolated or tested species.quinupristin/dalfopristin (rp 59500, synercid) is a parenteral streptogramin combination antimicrobial that possesses a synergistic and often bactericidal action against many grampositive species. in this study, a collection of 1270 uncommonly isolated or tested strains were evaluated for susceptibility to quinupristin/dalfopristin using agar dilution minimum inhibitory concentration (mic) methods described in the national committe for clinical laboratory standards. the greatest antimicrobial ac ...19968902412
molecular analysis of microflora associated with dentoalveolar abscesses.the microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16s rrna genes were randomly amplified by means of conserved eubacterial primers and cloned. restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16s rrna from the cultured bacteria was used to detect putative unculturable bacteria. clones representative of five predominant groups of uncultured organisms were sequenced. two were identified as porp ...19968904410
a study of the anaerobic bacterial flora of the female genital tract in health and disease.semi-quantitative and qualitative bacterial assessment of the vaginal and cervical flora of a total of 202 women was carried out over a period of six months to determine the bacterial flora in three groups of women and changes caused by prior use of antibiotics. the number was made up of 32 healthy volunteers, 80 women with gynaecological problems and 90 women with gynaecological infections who had had antibiotic treatment prior to this study. standard methods were used for the investigations. f ...19958907604
vaginal microflora associated with bacterial vaginosis in japanese and thai pregnant women.the vaginal flora of 118 japanese and 208 thai pregnant women were investigated for the presence of bacterial vaginosis (bv), bv-associated organisms, and bv-associated enzyme. a similar prevalence of bv was found among the japanese (13.6%) and thai women (15.9%). the microbial flora of women with bv were complex; the mean number of isolates recovered in the bv group was approximately 2 times more than that in a group of healthy women. prevotella species, porphyromonas species, peptostreptococcu ...19968909838
microbiology of infected atopic dermatitis.bacterial infections occur frequently in lesions of atopic dermatitis (ad). the objectives of the study were to establish the aerobic and anaerobic microbiology of secondarily infected ad.19968915731
microbiology of the transition from acute to chronic maxillary sinusitis.repeated aspirations of sinus secretions by endoscopy was performed in five patients over a period of 34-50 days and, ultimately, surgical drainage was done in three who presented with acute maxillary sinusitis that did not respond to antimicrobial therapy and became chronic. the aspirates were cultured for aerobic and anaerobic bacteria. most of the bacteria isolated from the first culture were aerobic or facultative bacteria: streptococcus pneumoniae (three isolates), haemophilus influenzae no ...19968918954
pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples.pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ...19968919784
[orbit complication of sinusitis. a report of 7 cases].orbital complications of sinusitis were reviewed. seven cases seen in a 6-year period from 1989 to 1994 were classified following chandler. the pathogenesis, classification, diagnosis, and treatment of orbital complications, which are rare and steadily decreasing in frequency, are discussed. because of its possible consequences, this complication should not be overlooked.19968924288
[occurrence of non-spore forming anaerobic bacteria in the upper airways of patients with chronic obstructive pulmonary disease].the aim of this study was to assess the occurrence of anaerobic non-sporeforming bacteria upper airways flora in the exacerbation of copd. sputum from 35 copd patients was sent to the laboratory in sterile, filled with co2 containers and cultured under anaerobic conditions. in all patients bacteriological tests were positive. the most common anaerobic bacteria were as follows: peptostreptococcus - in 28 pts, fusobacterium - in 27 pts, bacteroides - in 26 pts and prevotella in 24. less common wer ...19968926767
[the study of drug resistance in aerobic and anaerobic bacterial flora to selected antibacterial drugs].the aim of the study to determine the resistance to certain antibacterial drugs of the aerobic and anaerobic bacterial flora isolated from patients operated on for acute abdominal infections. the resistance was investigated by the disc diffusion method. among the isolated aerobes enterobacteriaceae, enterococcus spp, pseudomonas spp. staphylococcus spp were most frequent, and in the group of anaerobes the most frequent were bacteroides spp, peptostreptococcus spp and peptococcus spp. the sensiti ...19968926770
[antimicrobial activities of clarithromycin against clinical isolates].to examine the antimicrobial activity of clarithromycin (cam) against strains clinically isolated from outpatients in 1994, minimum inhibitory concentrations (mics) were determined for cam and the control drugs. the results were as follows; 1. mic50 and mic90 of cam were similar to those investigated in 1980's against many bacterial species. 2. cam showed strong antimicrobial activities against beta-lactamase producing moraxella subgenus branhamella catarrhalis, bordetella pertussis, campylobact ...19968935125
degradation of host protease inhibitors and activation of plasminogen by proteolytic enzymes from porphyromonas gingivalis and treponema denticola.bacterial proteases may participate in the pathogenesis of periodontal diseases through their action on host proteins. in the present study, the ability of selected periodontopathogens, as well as two proteases isolated from porphyromonas gingivalis and treponema denticola, to degrade host protease inhibitors was evaluated. the activation of human plasminogen by the two bacterial proteases was also investigated. proteolytic breakdown of host protease inhibitors (alpha-1-antitrypsin, antichymotry ...19968936322
the vaginal microflora of pig-tailed macaques and the effects of chlorhexidine and benzalkonium on this characterize normal vaginal microflora of pig-tailed macaques and to evaluate two commonly used intravaginal compounds, chlorhexidine (chg), a vaginal antiseptic (surgilube, e. fougera, melville, ny), and benzalkonium chloride (bzk) (spermicidal contraceptive) in this monkey model to assess effects on the vaginal microflora.19968946634
in vitro susceptibility of anaerobes to quinolones in the united states.the in vitro activity of early fluoroquinolone antibodies--including ciprofloxacin, ofloxacin, fleroxacin, pefloxacin, enoxacin, and lomefloxacin--against most anaerobes has been limited, a characteristic making them poor choices as antianaerobic agents. newer fluoroquinolones, including levofloxacin, sparfloxacin, and grepafloxacin, have moderate activity against anaerobes, including the bacteroides fragilis group as well as clostridium, peptostreptococcus, prevotella, and fusobacterium species ...19968953099
comparison of activities of trovafloxacin (cp-99,219) and five other agents against 585 anaerobes with use of three media.agar dilution testing was used to compare the in vitro activities of trovafloxacin and ofloxacin, ciprofloxacin, imipenem, metronidazole, and clindamycin against 585 anaerobes. the activity of trovafloxacin was superior to those of ofloxacin and ciprofloxacin, with 94%, 91%, 96%, 100%, 90%, and 100% inhibition of bacteroides fragilis, non-fragilis bacteroides species, peptostreptococcus magnus/peptostreptococcus micros, clostridium perfringens, prevotella bivia/prevotella disiens, and fusobacter ...19968953106
bacteriology of dentoalveolar abscesses in patients who have received empirical antibiotic therapy.we investigated aspirates of pus from dentoalveolar abscesses in 13 patients who had received empirical antibiotic therapy within 1 month before presentation at our facility. the antibiotics administered were lincomycin, amoxicillin, ampicillin, or a cephalosporin. information on antibiotic therapy was obtained from the patients' medical histories. cultures of all dentoalveolar abscesses yielded organisms. a total of 70 isolates (42 anaerobes and 28 facultative organisms) were recovered, represe ...19968953107
emerging resistance of anaerobic bacteria to antimicrobial agents in south previous studies, bacteroides fragilis group organisms isolated from korean patients were more frequently resistant to various antimicrobial agents, including clindamycin, than were isolates in other countries. a recent report of increased resistance of peptostreptococcus species prompted us to include such isolates in a study of antimicrobial susceptibility. anaerobes isolated in 1994 at a tertiary care hospital in seoul were tested by agar dilution method. none of the b. fragilis group orga ...19968953110
the adherence of periodontopathogens to periodontal probes. a possible factor in intra-oral transmission?periodontal probes have previously been shown to harbor several bacterial types or species after probing periodontally diseased pockets. this study aims to identify and quantify periodontopathogens that may adhere to a periodontal probe by culturing techniques. it also examines the probe's roughness on its capability to collect bacteria, comparing merrit-b probes (with deep indentations) with tps probes (with smooth surfaces). from the differential phase contrast microscopy findings it was seen ...19968959565
degradation of lactoferrin by periodontitis-associated bacteria.the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...19968961558
[successful scartectomy and cryoablation for ventricular tachycardia occurring late after correction of tetralogy of fallot].a 26-year-old man had a total repair of tetralogy of fallot at 1 year of age, and had redo surgery for restenosis of the right ventricular outflow tract and small residual vsd at 11 years of age. after the second operation, av block developed and an endocardial pacemaker system was implanted. for the last 3 years, he had mild febrile episodes, cough, occasional hemoptysis and paroxysmal ventricular tachycardia. because of his refractory tachycardia and suspected infective endocarditis, he was ad ...19968964997
[role of 67 gallium citrate and 99mtc-hmpao-labelled neutrophils in the integrated diagnostic imaging in a case of peptostreptococcus anaerobius hepatic abscess]. 19968966261
an rrna approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination.ruminal amino acid degradation is a nutritionally wasteful process that produces excess ruminal ammonia. monensin inhibited the growth of monensin-sensitive, obligate amino acid-fermenting bacteria and decreased the ruminal ammonia concentrations of cattle. 16s rrna probes indicated that monensin inhibited the growth of peptostreptococcus anaerobius and clostridium sticklandii in the rumen. clostridium aminophilum was monensin sensitive in vitro, but c. aminophilum persisted in the rumen after m ...19968975611
effect of 2-bromoethanesulfonic acid and peptostreptococcus productus atcc 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis.evidence is provided that reductive acetogenesis can be stimulated in ruminal samples during short-term (24-h) incubations when methanogenesis is inhibited selectively. while addition of the reductive acetogen peptostreptococcus productus atcc 35244 alone had no significant influence on ch4 and volatile fatty acid (vfa) production in ruminal samples, the addition of this strain together with 2-bromoethanesulfonic acid (bes) (final concentration, 0.01 or 0.03 mm) resulted in stimulation of acetic ...19978979351
[antimicrobial activity of cefodizime against clinical isolates].in order to evaluate antimicrobial activity of cefodizime (cdzm), minimum inhibitory concentrations (mics) of cdzm and control drugs were determined against clinical isolates collected from nation-wide medical institutions and in our laboratory from september to december of 1992 and from september to december of 1995. the results are summarized as follows: 1. bacterial species with no or few strains resistant to cdzm included streptococcus pyogenes, haemophilus influenzae, citrobacter koseri, pr ...19968986558
conformational analysis of peptides corresponding to all the secondary structure elements of protein l b1 domain: secondary structure propensities are not conserved in proteins with the same fold.the solution conformation of three peptides corresponding to the two beta-hairpins and the alpha-helix of the protein l b1 domain have been analyzed by circular dichroism (cd) and nuclear magnetic resonance spectroscopy (nmr). in aqueous solution, the three peptides show low populations of native and non-native locally folded structures, but no well-defined hairpin or helix structures are formed. in 30% aqueous trifluoroethanol (tfe), the peptide corresponding to the alpha-helix adopts a high po ...19979007989
contemporary management of deep neck space infections.deep neck infections continue to be seen despite the wide use of antibiotics. these infections follow along fascial planes to create deep neck space abscesses. the clinical presentation often points to the space involved. understanding the regional anatomy gives the surgeon the ability to treat these grave infections. the records of 24 patients with a diagnosis of deep neck space abscess admitted to hermann hospital between 1988 and 1993 were reviewed. fifty percent of the patients had received ...19979018251
microbiology of otitis externa.microbiologic and clinical data from 26 patients with otitis externa were prospectively evaluated. specimens were processed for aerobic and anaerobic bacteria. bacterial growth was noted in 23 specimens. a total of 33 aerobic and 2 anaerobic bacteria were recovered. aerobic bacteria only were isolated in 21 (91%) patients, anaerobic bacteria only in 1 (4%), and mixed aerobic and anaerobic bacteria in 1 (4%). the most common isolates were pseudomonas aeruginosa (14 instances), staphylococcus aure ...19979018252
electrophoresis of whole-cell soluble proteins of microorganisms isolated from bacteremias in endodontic therapy.we have previously demonstrated that anaerobic bacteria are the microorganisms most frequently isolated from blood following endodontic therapy of teeth with apical periodontitis. phenotypic characterisation of the isolates suggested that the bacteria in the blood originated from the root canal. the present experiment using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was carried out in an effort to verify these findings, and to further study the microorganisms involved i ...19969021323
development and evaluation of a selective and differential medium for the primary isolation of peptostreptococcus micros.peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. a selective and differential medium for the primary isolation of p. micros was developed and evaluated. columbia cna agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (p. micros medium: pmm). p. micros has a chara ...19969028263
evaluation of the anaeropack system for growth of clinically significant anaerobes.the anaeropack (mitsubishi gas chemical america, inc., new york, n.y.) system was compared with the gaspak (becton dickinson microbiology systems, cockeysville, md.) system and a conventional anaerobe chamber to evaluate the ability of the anaeropack system to support the growth of clinically significant anaerobes. the anaeropack system requires no catalyst or water, produces no hydrogen, and is oxygen absorbing and carbon dioxide generating. it is simple to use and reduces preparation time to a ...19979041388
the clinical, microbial, and host response characteristics of the failing implant.the goal of this study was to provide new data regarding levels of inflammatory and growth factor mediators and bacterial pathogens associated with failing implants, as compared to healthy implants. twenty-one patients with failing implant sites (group 1) and 8 patients with only healthy implants (group 2) were included. fifteen of the 21 failing implant patients (group 1) also presented with at least one stable nondiseased implant. plaque samples were examined, using dna oligonucleotide probes ...19979048452
equilibrium and pre-equilibrium fluorescence studies on the interaction between protein l and kappa light chain. 19979056936
aerobic and anaerobic microbiology of bacterial tracheitis in children.establish the aerobic and anaerobic microbiology of bacterial tracheitis in children.19979061728
anaerobic cocci of clinical importance.the current knowledge is reviewed concerning the anaerobic cocci, in particular those of clinical relevance. the anaerobic cocci are defined and their current taxonomic positions discussed. it is clear that new genera and species await to be characterised fully. the overwhelming majority found in clinical material belong to the genus peptostreptococcus, with the remainder belonging to the veillonellae and, possibly, ruminococci. human infections with other anaerobic cocci are extremely rare. the ...19969069108
inhibitory effects of metronidazole on anaerobic metabolism of phenylalanine and leucine by peptostreptococcus anaerobius.the metabolism of phenylalanine and leucine by peptostreptococcus anaerobius and the effects of metronidazole on this metabolism were studied. the cells metabolized both phenylalanine and leucine under strict anaerobic conditions and produced hydroxylated products (i.e. phenyllactate from phenylalanine and hydroxyisocaproate from leucine). other products were phenylpropionate, cinnamate and phenylacetate from phenylalanine; isocaproate, alpha-ketoisocaproate and isovalerate from leucine. metroni ...19979069531
septic arthritis after arthroscopic anterior cruciate ligament reconstruction. diagnosis and management.we performed a retrospective study of knee joint infections after arthroscopic anterior cruciate ligament reconstruction at our institution. two thousand five hundred anterior cruciate ligament reconstructions were performed between 1988 and 1993. seven (0.3%) patients experienced postoperative deep infections of the knee. all anterior cruciate ligament reconstructions were performed using arthroscopically assisted techniques. six (86%) of these patients had concomitant open procedures performed ...19979079185
solution structure of the albumin-binding ga module: a versatile bacterial protein domain.the albumin-binding ga module is found in a family of surface proteins of different bacterial species. it comprises 45 amino acid residues and represents the first known example of contemporary module shuffling. using 1h nmr spectroscopy we have determined the solution structure of the ga module from protein pab, a protein of the anaerobic human commensal and pathogen peptostreptococcus magnus. this structure, the first three-dimensional structure of an albumin-binding protein domain described, ...19979086265
a comparison of susceptibility results of the bacteroides fragilis group and other anaerobes by traditional mic results and statistical methods.antimicrobial susceptibility is compared by mic values reflecting activity by weight and/or percentage of strains resistant. this study establishes these parameters in vitro in the traditional fashion and extends analysis statistically for various groups of anaerobic bacteria. mode mic, mic50, mic90 values and percentage of strains resistant indicated comparable activity for most of the beta-lactams. geometric and arithmetic mics suggested ceftizoxime to be the most active beta-lactam. statistic ...19979096180
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