TitleAbstractYear(sorted ascending)
[the role anaerobes in dermatitis digitalis et interdigitalis in cattle].altogether 52 tissue samples and swabs from the corium of the claw of diseased cattle were investigated. microbiological examinations revealed the presence of 11 strictly anaerobic bacterial strains that belonged to the species peptococcus asacharolyticus, pc. sacharolyticus, peptostreptococcus anaerobius, bacteroides asacharolyticus and b. melaninogenicus. the remaining isolates were classified as bacteroides, fusobacterium, streptococcus and clostridium. in the above-mentioned strains observat ...19938259638
role of suspected periodontopathogens in microbiological monitoring of periodontitis.periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. although a specificity to some degree is found for actinobacillus actinomycetemcomitans in localized juvenile periodontitis (ljp), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. what we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshol ...19938260004
microbiology of the pericoronal pouch in mandibular third molar pericoronitis.the microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. the pericoronal pouch was sampled with paper points in a) 8 patients without mandibular third molar pericoronitis and b) 6 patients with mandibular third molar pericoronitis. under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), mot ...19938265205
anaerobic infection in chronic maxillary sinusitis.1) ten per cent of cases of chronic maxillary sinusitis in a series of 50 cases treated in the ent department of dayanand medical college, ludhiana, were caused by anaerobic infection. 2) the causative anaerobic micro-organisms encountered were peptostreptococcus and clostridium species. 3) treatment should, therefore, include metronidazole, especially in those cases refractory to conventional antibiotic therapy.19938270303
treatment of post-cesarean section endometritis with ampicillin and sulbactam or clindamycin and gentamicin.seventy-seven patients were prospectively enrolled in a randomized clinical trial to compare two antimicrobial regimens for the treatment of post-cesarean section endometritis. the two groups were not significantly different with respect to age, race, gravidity, parity, hours in labor, cesarean section indication, preoperative or postoperative hemoglobin/hematocrit, pretreatment white blood cell count or pretreatment temperature. pretreatment urine, blood and endometrial cultures were obtained. ...19938277478
in vitro activity of bay y3118 against anaerobic bacteria.the antimicrobial activity of a new quinolone, bay y3118, was determined against 326 strains of anaerobic bacteria and compared with the activities of ampicillin-sulbactam, cefotetan, clindamycin, imipenem, metronidazole, and sparfloxacin. the national committee for clinical laboratory standards-approved wadsworth agar dilution technique with brucella-laked blood agar was used throughout the study. breakpoints used to determine the percent susceptible were 2 micrograms/ml for bay y3118 and sparf ...19938285647
piperacillin and tazobactam versus clindamycin and gentamicin in the treatment of hospitalized women with pelvic infection. the piperacillin/tazobactam study compare the efficacy and safety of a combination of piperacillin and tazobactam with that of clindamycin and gentamicin in the treatment of hospitalized women with infections of the upper genital tract.19948290195
impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis.the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ...19948195981
bacterial endocarditis in patients with chronic liver disease.although patients with cirrhosis have an increased susceptibility for bacterial infections, endocarditis complicating cirrhosis has been reported only infrequently. in this study, our objective was to determine whether, bacterial endocarditis is, in fact, a complicating factor in cirrhosis.19948198106
[antimicrobial activities of cefuroxime against recent clinical isolates].antimicrobial activity of cefuroxime axetil (cxm-ax) was compared with those of other cephem antibiotics against clinically isolated strains obtained mainly from outpatients of our center in a period from january to september of 1990 and 1993. minimum inhibitory concentrations were determined and the following results were obtained. 1. the results suggested that, compared with reports of studies conducted with clinical isolates in early 1980's, mic80 of cxm were equal to or lower against staphyl ...19948201767
in vitro antibacterial activity of fk037, a new parenteral broad-spectrum cephalosporin, against recent clinical isolates in the fields of obstetrics and gynecology.the antibacterial activity of a new parenteral cephalosporin, fk037 was assessed against recent aerobic and anaerobic strains isolated from patients in the fields of obstetrics and gynecology during the period between january 1992 and june 1993. the mics of fk037 for 90% of the clinical isolates tested were 0.10 microgram/ml for escherichia coli and klebsiella pneumoniae, 0.20 microgram/ml for streptococcus agalactiae, 0.39 microgram/ml for gardnerella vaginalis, 0.78 microgram/ml for staphyloco ...19948205934
[anaerobic respiratory infection--evaluation of methods of obtaining specimens].anaerobic cultures were performed on various kinds of specimens obtained from 67 patients with respiratory infections. the infections consisted of 33 cases of pneumonia, 14 cases of lung abscess, 6 cases of pyothorax, 9 cases of chronic lower respiratory tract infection, and 5 cases of infection of pulmonary cyst. specimens included bronchoscopic specimens obtained using a single tube or protected catheter brush (pcb), transtracheal aspirate, percutaneous lung aspirate, aspirate using a suction ...19948207291
a quantitative comparison of selected bacteria in human carious dentine by microscopic counts.the levels of selected cariogenic and obligately anaerobic bacteria have been compared in carious dentine taken from fissures, smooth surfaces, and root surfaces. the numbers of infected dentinal tubules were determined by immunohistological staining using species-specific antisera against selected bacteria. selective localization was observed; mutans streptococci were the predominant bacteria in dentine from fissures and smooth surface coronal caries, but not from root surface caries. the propo ...19948033185
bacteria invading periapical cementum.the aim of this study was to determine whether microorganisms invade periapical cementum of human teeth from the adjacent periapical lesions. we therefore attempted to isolate microorganisms from periapical cementum through the adoption of standard anaerobic procedures for obligate anaerobes. samples of cementum were taken from 10 amputated tooth roots at the time of apicoectomy. from two of these samples, bacteria were recovered after anaerobic incubation, but no bacteria were recovered after a ...19948035155
identification of five peptostreptococcus species isolated predominantly from the female genital tract by using the rapid id32a system.the rapid id32a kit (biomérieux vitek, inc., hazelwood, mo.) was evaluated for its ability to identify peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (virginia polytechnic institute), the current "gold standard" method. a total of 5 peptostreptococcus american type culture collection strains and 95 clinical isolates comprising peptostreptococcus anaerobius, p. asaccharolyticus, p. magnus, p. micros, and p. prevotii isolates were included for ...19948051260
case report: anaerobic meningitis caused by peptostreptococcus magnus after head and neck surgery.although anaerobic bacterial meningitis is uncommon, patients subjected to resection of head and neck malignancy appear at special risk. in this article, the authors report on a 72-year-old man in whom meningitis developed after extensive resection of the right sinuses for squamous cell carcinoma; initial treatment consisted of intravenous vancomycin and ceftazidime. intravenous penicillin g was added after the fortuitous early finding of intracellular cocci in wright-giemsa stained cerebral spi ...19948074138
changes in vaginal flora during pregnancy and association with preterm birth.a prospective study of the vaginal flora changes between midtrimester and labor was undertaken in 560 women; 337 had intact membranes and were in labor when swabs were taken. of these, 45 delivered preterm. gardnerella vaginalis, ureaplasma urealyticum (both associated with preterm birth when present in midtrimester), and mycoplasma hominis commonly persisted between midtrimester and labor, particularly in the preterm cohort (24 [65%] of 37 midtrimester isolates), and these organisms were seldom ...19948077737
iron-sulfur cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus: correlation of the cluster type with enzymatic activity.investigations were performed with regard to the function of the iron-sulfur cluster of l-serine dehydratase from peptostreptococcus asaccharolyticus, an enzyme which is novel in the class of deaminating hydro-lyases in that it lacks pyridoxal-5'-phosphate. anaerobically purified l-serine dehydratase from p. asaccharolyticus revealed epr spectra characteristic of a [3fe-4s]+ cluster constituting 1% of the total enzyme concentration. upon incubation of the enzyme under air the intensity of the [3 ...19948082806
[a case of niddm with non-clostridial gas-producing infection in the lower limb--the effects of hyperbaric oxygen therapy].a 36-year-old male was admitted because of swelling and pain in the lower left limb. he had been diagnosed as having non-insulin dependent diabetes mellitus 4 years previously and had been treated unsuccessfully with insulin. since gas formation was radiographically demonstrated in the soft tissue of the left foot, we treated him with antibiotics and hyperbaric oxygen under the diagnosis of gas gangrene. despite this therapy, the gangrenous lesions progressed. amputation of the left foot was nec ...19948151155
[antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections].to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ...19948151907
relationship between salivary blood group antigens, microbial flora and periodontal condition in young investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ...19948157769
protein pab, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling.some strains of the anaerobic human commensal and pathogen peptostreptococcus magnus bind human serum albumin (hsa), whereas other strains of this species express protein l, an immunoglobulin light chain-binding surface protein. a novel hsa-binding protein called protein pab was purified in one step from the culture supernatant of an hsa-binding strain of p. magnus by affinity chromatography on hsa-sepharose. the apparent size of the molecular was 47 kda on sds-polyacrylamide gel electrophoresis ...19948163519
delta 22-beta-muricholic acid in monoassociated rats and conventional rats.bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ...19948177022
antibacterial activity of clavulanate and tazobactam on peptostreptococcus spp. 19948182024
recombinant proteins l and lg. two new tools for purification of murine antibody fragments.several bacterial cell wall proteins, like proteins a and g, with valuable affinity for immunoglobulins have been discovered and are currently employed in immunological techniques. in 1988, protein l, a bacterial cell wall protein with ig-binding capacity, was isolated from the anaerobic bacterial species peptostreptococcus magnus. binding data with immunoglobulin fragments suggested that protein l could selectively bind the variable region of human kappa light chains. more recently a recombinan ...19947736533
an unclassified eubacterium taxon in acute dento-alveolar abscess.the microflora of pus samples aspirated from 50 acute dento-alveolar abscesses was examined. a total of 143 bacterial strains was isolated, consisting predominantly of prevotella spp., alpha-haemolytic streptococcus spp., peptostreptococcus spp. and eubacterium spp. an unclassified asaccharolytic eubacterium taxon was encountered in 17 (34%) of the abscesses. this taxon was found to have a positive association with fusobacterium spp. and a negative association with alpha-haemolytic streptococcus ...19948107059
electrogenic glutamine uptake by peptostreptococcus anaerobius and generation of a transmembrane potential.peptostreptococcus anaerobius converted glutamine stoichiometrically to ammonia and pyroglutamic acid, and the eadie-hofstee plot of glutamine transport was biphasic. high-affinity, sodium-dependent glutamine transport (affinity constant [kt] of 1.5 microm) could be driven by the chemical gradient of sodium, and more than 20 mm sodium was required for half-maximal velocity. high-affinity glutamine transport was not stimulated or inhibited by a membrane potential (delta psi). low-affinity glutami ...19948113169
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...19948123556
bacterial colonization and degradation of demineralized dentin matrix in situ.lesion formation in dentin involves demineralization followed by degradation of the exposed organic matrix. proteinases from microorganisms present in the dentin are believed to play an important role in the breakdown of the dentinal collagen. in this study, the microflora colonizing decalcified dentin matrix, placed in situ, was identified. the gelatinolytic activity of the isolated strains was assessed and related to the degradation of the dentin matrix. the predominant species found were stre ...19948124693
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...19948126176
determination of 16s ribosomal rna sequences of all members of the genus peptostreptococcus and their phylogenetic position.a comparative analysis of 16s ribosomal rna sequences of all peptostreptococcus species revealed that most members of the genus peptostreptococcus should be divided into many different genera. the relationship between clostridia and peptostreptococci was analysed to find the phylogenetic position of peptostreptococci.19948132148
effect of different combinations of antibiotics on experimental septic peritonitis in rabbits.this study was undertaken to evaluate the efficacy of different combinations of antimicrobial agents in the treatment of experimental septic peritonitis. two hundred rabbits, divided into ten groups of 20 rabbits each, were used. septic peritonitis was provoked in two stages: treatment of animals by mechanical lavage or antibiotics was performed during the first stage; blood cultures, isolation and identification of aerobic and anaerobic bacteria in the peritoneal cavity were performed during th ...19947819597
[treatment of puerperal endometritis. evaluation of the efficacy and safety of clindamycin + gentamycin vs. penicillin + chloramphenicol + gentamycin].this was a prospective, single-blind, comparative study in patients with diagnosis of puerperal endometritis, carried out at the loayza hospital in lima, peru. the objective of this study was to evaluate the efficacy and safety of clindamycin and gentamicin in the management of endometritis vs. penicillin, chloramphenicol and gentamicin for 10 days. sixty-five patients were enrolled and 62 were evaluable for efficacy. both treatment groups were comparable in the pre-treatment period in terms of ...19947821833
on the interaction between single chain fv antibodies and bacterial immunoglobulin-binding proteins.using four bacterial immunoglobulin-binding proteins, we have analyzed the binding characteristics of a panel of 34 human single chain fv antibodies, expressed in e. coli and with known specificity and sequence. several of the single chain fv antibodies showed affinity for staphylococcal protein a and peptostreptococcal protein l, but not for the streptococcal proteins g or h. the affinity of the binding was higher for protein l (4.5 and 1.4 x 10(9) m-1) than for protein a (7.7 and 6.7 x 10(8) m ...19947822821
[susceptibility of strict anaerobic bacteria to antibiotics in france: a multicenter study].during 1992, the in vitro antibiotic susceptibility of 462 anaerobic bacteria was performed in 7 hospitals, by the reference agar dilution method. among the 222 bacteroides fragilis group strains, only one bacteroides fragilis strain was resistant to imipenem and all bêtalactams, even combined with bêtalactamase-inhibitors while metronidazole resistance could not be detected. one major outer membrane protein (probably a porin) was lacking in some of the six amoxicillin-clavulanic acid resistant ...19947824321
in vitro activity of du-6859a against anaerobic bacteria.the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ...19947840599
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...19947851086
microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ...19947853132
peptostreptococcal infection in children.680 peptostreptococcus sp. (ps) were recovered from 598 (34%) of 1,750 specimens obtained from 554 patients. they included 103 ps asaccharolyticus, 74 ps magnus, 56 ps prevotii, 51 ps micros, 46 ps anaerobius, 11 ps morbilorum, and 10 ps saccharolyticus. most infections were polymicrobial (in 553 instances or 92%) but in 45 (8%), ps were recovered in pure culture. most ps were isolated from abscesses (237), ears (104), peritoneal fluid (95), lung infections (66), bone (30) and sinuses (24). pred ...19947855547
bacteriological study of bronchoalveolar lavage in immunocompromised hosts.sixty bronchoalveolar lavage samples collected from lung cancer patients attending the pulmonary tuberculosis and chest diseases unit of the kasturba medical college hospital, manipal; were cultured for both the aerobic and anaerobic organisms. fifty nine samples yielded bacteria in pure culture. pseudomonas aeruginosa (34.8%) was the commonest aerobe, peptostreptococcus was the commonest anaerobe (45.2%) isolated. bacteroides fragilis was isolated in (23.8%) of cases. gentamycin was found to be ...19947868174
the gingival immune response to periodontal pathogens in juvenile periodontitis.a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ...19947870467
major methodology-dependent discordant susceptibility results for bacteroides fragilis group isolates but not other anaerobes.two standardized susceptibility test methods, a broth microdilution (bmd) and agar dilution (ad) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. against the 339 strains of the bacteroides fragilis group bmd minimum inhibitory concentration (mic) values were lower than those from ad testing for all the beta-lactams. overall for the b. fragilis group and the beta-lactams, the mode mic valu ...19947874880
study of gene transfer in vitro and in the digestive tract of gnotobiotic mice from lactococcus lactis strains to various strains belonging to human intestinal flora.the use of genetically modified organisms (gmo) in dairy products requires evaluation of the dna transfer capacity from such organisms among the human intestinal microflora. thus, both in vitro and in vivo [in the digestive tract (dt) of mice] transfer from lactococcus lactis donor strains of the conjugative plasmid pil205 (cmr) and the non-conjugative plasmid pil253 (emr) to: (1) recipient strains isolated from human faecal flora bacteroides sp., bifidobacterium sp., peptostreptococcus sp. (str ...19947921350
anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ...19947924212
peritonitis following endoscopic polypectomy in a peritoneal dialysis patient: the need for antibiotic prophylaxis. 19947927824
evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates.the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ...19947933531
the functional units of a peptostreptococcal protein l.protein l is a cell-surface protein from peptostreptococcus which interacts with immunoglobulin kappa light chains. a gene from peptostreptococcus strain 3316 coding for protein l and fragments thereof were expressed in escherichia coli. the peptides were examined for binding to immunoglobulin and serum albumin. the four c units were shown to be responsible for binding to immunoglobulin and the four d units for binding to albumin. this protein l molecule therefore binds to albumin at a site sepa ...19947934898
changes in root canal microbiota during the development of rat periapical lesions.periapical lesions are reproducibly induced in rats by pulp exposure and infection from the oral cavity. lesions expand rapidly between day 7 and day 15-20 (active phase), with slowed expansion thereafter. in the present study we characterized the root canal microbiota present during the active phase of lesion development in this system. the mandibular first molars of sprague-dawley rats were exposed on day 0. the teeth were extracted after 7 days (n = 10 animals) and 15 days (n = 10), and the m ...19947936717
diagnosis and management of abscesses in the basal ganglia and thalamus: a survey.a review is made of the current management strategies of abscesses in basal ganglia and thalamus, based on a review of the literature and three of our own cases. clinical picture, aetiology, diagnostic, surgical treatment and outcome are discussed. stereotactic abscess puncture in combination with temporary drainage and rinsing of the abscess cavity in combination with systemic medication of antibiotics has become the management of choice with satisfactory results.19947942190
three-dimensional solution structure of an immunoglobulin light chain-binding domain of protein l. comparison with the igg-binding domains of protein g.protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. it has affinity for immunoglobulin (ig) through interaction with framework structures in the variable ig light chain domain. the ig-binding activity is located to five homologous repeats called b1-b5 in the n-terminal part of the protein. we have determined the three-dimensional solution structure of the 76 amino acid residue long b1 domain using nmr spectrosc ...19947947810
study on treatment of bacterial vaginosis with oral administration of metronidazole or cefdinir.bacterial vaginosis (bv) is considered to be one of the most common vaginal infections in women. fifteen symptomatic women with bv were enrolled in this study. ten patients with the diagnosis of bv were treated with 10 days oral administration of metronidazole (mtn), 500 mg twice a day, and five patients with cefdinir (cfdn), 300 mg three times a day. in the mtn therapy, the rate of abnormal vaginal discharge subjectively decreased from 100 to 60%, the rate of abnormal vaginal discharges objecti ...19947956461
rapid diagnosis of anaerobic gram-positive cocci by salt tolerance.the maximum salt tolerance was 2.5% in cases of peptostreptococcus anaerobius and peptococcus prevotii, 3% in ruminococcus albus, 4% in acidaminococcus fermentans, peptococcus niger and megasphaera elsdenii and 5% in peptococcus magnus. the biochemical tests being largely inactive and time consuming the findings of salt tolerance if combined with those of morphological data provide clue to the rapid identification of gram-positive anaerobic cocci.19947963612
[etiologic factors in acute inflammatory states of the abdominal cavity].three hundred and two samples of biological material from patients operated because of acute inflammatory states of abdominal cavity were investigated. both aerobic and anaerobic bacteria were investigated. among aerobic flora most frequently occurred enterococci, e. coli and pseudomonas. bacteroides and peptostreptococcus were most frequently occurring anaerobes. mixed flora was seen very often. isolated microorganisms were tested for susceptibility to ciprofloxacin, cefoperazone, cefotaxime, g ...19947967931
increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria.a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ...19947979307
nucleotide sequence of the gene for peptostreptococcal protein l.a gene bank of peptostreptococcus magnus dna was established using an e. coli host-vector system. western blot analysis identified a clone expressing protein l which bound to the light chain of human immunoglobulins. dna sequence determination and analysis revealed an open reading frame of 992 amino acids, giving a theoretical secreted protein of 106 kd with a pl of 4.67.19947987012
[brain abscess complicating dental caries in children].brain abscess following dental or periapical infection is rare in childhood. this report describes brain abscesses found in two children with dental caries. case 1.--a 12 year-old boy was admitted because he had suffered from acute meningitis for 3 days. clinical examination showed symptoms of meningitis plus palsy of the right third and fourth cranial nerves and of the left facial nerve, and a defect in the left temporal field. funduscopic examination showed papilledema; ct scan and mri showed ...19947987445
prophylactic antibiotic treatment in therapeutic or complicated diagnostic ercp: results of a randomized controlled clinical study.the present study evaluated the effects of prophylactic administration of cefotaxime in patients undergoing therapeutic or complicated diagnostic ercp. one hundred patients were randomized to two groups of 50 patients each. patients in group 1 received an intravenous infusion of 2 g cefotaxime 15 minutes before endoscopy; patients in group 2 did not receive an intravenous antibiotic before ercp (control group). blood samples were drawn for bacteriologic cultures before endoscopy and 5, 15, 30, a ...19947988813
evaluation of the antimicrobial effectiveness of citric acid and sodium hypochlorite on the anaerobic flora of the infected root canal.this study was designed to evaluate the antimicrobial effectiveness of 25% citric acid, compared with 2.5% sodium hypochlorite, after time intervals of 5, 15, 30 and 60 min. anaerobic bacterial species were isolated from infected root canals and categorized according to gram stain as gram-positive cocci, gram-negative cocci, gram-positive rods and gram-negative rods. the experimental method used was similar to that proposed by the german society for hygiene and microbiology (dghm) with slight mo ...19947995646
[an evaluation of pathogens in patients with bronchopulmonary infection by transtracheal aspiration: december 1978-march 1993].we performed transtracheal aspiration (tta) in 1165 patients, who were suspected to have bronchopulmonary infection, from december 1978 to march 1993. we isolated pathogens from tta in 806 patients (69.2%). we isolated h. influenzae (62 cases), s. pneumoniae (39 cases) and m. catarrhalis (24 cases) in patients with acute bronchitis, s. pneumoniae (65 cases), alpha-streptococcus sp. (52 cases), h. influenzae (32 cases) and s. aureus (29 cases) in patients with pneumonia or lung abscess and h. inf ...19947996025
distribution of clindamycin in cortical bone during direct local infusion of the canine tibia.the distribution of clindamycin in tibial cortical bone, administered via direct local infusion with an implantable pump, is described. clindamycin concentrations in cortical bone were measured after 3, 7, and 21 days of intraosseous infusion. the tibia were divided into four quadrants relative to the outflow infusion catheter site located in the medial aspect of the mid-diaphysis. a gradient of 5-30 mm from the infusion site was documented in all four quadrants (proximal lateral, proximal media ...19947996320
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...19948004324
the clinical importance of gram-positive anaerobic cocci isolated at st bartholomew's hospital, london, in 1987.the clinical importance of the gram-positive anaerobic cocci (gpac) isolated in 1987 at st bartholomew's hospital, london, is assessed. of about 800 anaerobic isolates, 209 (27%) were gpac, of which 67 (32%) were from abscesses and 22 (11%) were in pure growth. four species comprised 77% of the 168 isolates available for study: peptostreptococcus magnus (55 isolates, 33%), p. micros (23, 14%), p. asaccharolyticus (24, 14%) p. asaccharolyticus (24, 14%) and p. anaerobius (27, 16%). different spec ...19948006943
penicillin g and penicillin g-tinidazole treatment of experimentally induced summer mastitis--effect on elimination rates of bacteria and outcome of the disease.two udder hind quarters of ten pregnant heifers were inoculated experimentally with a combination of actinomyces pyogenes, peptostreptococcus indolicus and fusobacterium necrophorum at a dose of 9 x 10(8) colony forming units (cfu) of each species of bacteria. the development of aerobic-anaerobic mastitis was followed-up clinically and through analysis of udder secretion samples. all heifers developed acute clinical mastitis. thick, purulent secretions with foul odour were already present 24 h a ...19949133056
[right-sided infective endocarditis with ventricular septal defect].two patients underwent surgical treatment for right-sided infective endocarditis with ventricular septal defect. in both cases, blood cultures showed peptostreptococcus, and the operation was performed at non-active phase after antibiotics therapy. the case 1 was a 7-year-old girl who was observed a vegetation on the chorda of the anterior paillary muscle by echocardiography. the defect was directly closed and the vegetation was excised. the case 2 was 22-year-old female who had been diagnosed o ...19949423075
the antimicrobial activity of essential oils and essential oil components towards oral bacteria.a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ...19947478759
positive and negative associations between bacterial species in dental root canals.significant associations have been previously reported between certain pairs of bacterial species isolated from human dental root canals. the aim of this study was to examine microbiologically a more extensive series of cases, with particular reference to obligate anaerobes which accounted for 64% of total isolations. a total of 65 different species was isolated and individual root canals yielded a maximum of eleven bacterial species. highly significant positive associations (p < 0.001) were fou ...19947700162
evaluation of the etest for antimicrobial spectrum and potency determinations of anaerobes associated with bacterial vaginosis and hundred ninety-seven anaerobic organisms (24 gardnerella vaginalis, 16 mobiluncus spp., 19 peptostreptococcus spp., 20 lactobacillus spp., 20 prevotella bivia/disiens, 81 bacteroides fragilis group, 12 clostridium spp., and five fusobacterium spp.) were processed by the etest (ab biodisk, solna, sweden) and a reference (brucella blood agar) method against 10 antimicrobial agents. for the bacterial vaginosis-associated pathogens, the etest was more reproducible and correlated acceptably with ...19947705035
associations of specific bacteria with some endodontic signs and symptoms.although a wide range of bacterial species has been isolated from infected dental root canals it remains necessary to determine whether any particular group of such bacteria is associated with specific endodontic symptoms and clinical signs. in this study 30 root canals were examined microbiologically; of these, 14 were associated with pain, 20 with tenderness to percussion, 23 presented with wet root canals, seven with swelling, five with purulent exudate and four with a sinus. clinical and mic ...19947751061
demonstration of human immunoglobulin g fc-binding activity in oral bacteria.nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ...19947496956
in vitro comparison of the effectiveness of three surface disinfectants.choosing a general purpose surface disinfectant for the dental office environment is difficult because of the wide range of products available, varying claims by manufacturers and contradictory reports in the literature regarding product efficacy. this study tested the antimicrobial effectiveness of diluted-for-use o-phenyl-phenol (omni), didecyl dimethyl ammonium (basic g) and isopropanol (virahol). products were tested for antimicrobial activity at 0, 12 and 24 hours, 3, 6 and 8 days. o-phenyl ...19947523623
techniques for controlling variability in gram staining of obligate anaerobes.identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ...19957538512
translation of trpg in bacillus subtilis is regulated by the trp rna-binding attenuation protein (trap).trpg of bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and l-tryptophan. expression of trpg is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. examination of both transcriptional and translational gene fusions to lacz involving trpg and direct measurements of trpg mrna levels and trpg polypeptide accumulation demonstrated that translation of trpg is regulated by trypt ...19957543470
role of the reference laboratory in susceptibility testing of anaerobes and a survey of isolates referred from laboratories in england and wales during 1993-1994.methods for susceptibility testing of anaerobes and their patterns of susceptibility remain debatable. the responsibilities of the public health laboratory service anaerobe reference unit include identification of referred isolates; development of methods; monitoring of the occurrence of anaerobic infections; performance of taxonomic studies; maintenance of a reference collection; and monitoring of the antibiotic susceptibility of clinical isolates. selection of antibiotics for anaerobic infecti ...19957548547
bacteriology of chronic otitis media, chronic sinusitis, and paranasal mucopyocele in japan.a bacteriologic study of cases of chronic otitis media, chronic sinusitis, and paranasal mucopyocele diagnosed at gifu university hospital was performed between 1989 and 1992. bacteria were isolated from all of 13 samples of discharge from the ears of patients with chronic otitis media, all of 14 mucosal biopsy specimens from patients with chronic sinusitis, 8 of 10 aspirate samples from patients with chronic sinusitis, and 9 of 15 aspirates samples from patients with mucopyocele. cultures often ...19957548557
a retrospective review of cases of anaerobic empyema and update of bacteriology.we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ...19957548560
role of bacterial vaginosis in pelvic inflammatory disease.pelvic inflammatory disease (pid) is a frequent infection in sexually active young women and results in adverse sequelae, including tubal-factor infertility and ectopic pregnancy. in the 1970s investigations using culdocentesis demonstrated that anaerobic bacteria played an important role in the etiology of pid. this finding has subsequently been confirmed by studies utilizing laparoscopy and/or endometrial biopsy to obtain specimens directly from the upper genital tract (uterine cavity and fall ...19957548573
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...19957548575
foot infections in diabetic patients: the role of anaerobes.the prevalence of anaerobic bacteria in cultures of specimens from foot infections in diabetic patients is dependent upon the method of obtaining the specimen, the care with which it is transported anaerobically, and the sophistication of the laboratory methods. the rate at which anaerobes are isolated with use of the best methods ranges from 74% to 95% of patients, but it is only 41% to 53% in clinical studies of patients with limb-threatening infection. the mean number of bacterial isolates fr ...19957548576
a phage display system for studying the sequence determinants of protein folding.we have developed a phage display system that provides a means to select variants of the igg binding domain of peptostreptococcal protein l that fold from large combinatorial libraries. the premise underlying the selection scheme is that binding of protein l to igg requires that the protein be properly folded. using a combination of molecular biological and biophysical methods, we show that this assumption is valid. first, the phage selection procedure strongly selects against a point mutation i ...19957549875
the microbiology and antibiotic treatment of peritonsillar abscesses.pus from 53 peritonsillar abscesses was cultured and associations between the microbiological results and clinical data were investigated with the aim of developing a clinical protocol for treatment. a positive culture grew in 85% of quinsies and of these 16% produced aerobes and 84% anaerobes. penicillin-resistant organisms were grown from 32% of patients and all but one of these organisms (haemophilus influenzae) was sensitive to metronidazole. there was no association between clinical present ...19957554331
microbiology of secondary bacterial infection in scabies lesions.aerobic and anaerobic bacteria were grown from specimens obtained from 30 children with secondarily infected scabies lesions. aerobic or facultative bacteria only were present in 14 (47%) patients, anaerobic bacteria only were present in 6 (20%) patients, and a mixed anaerobic-aerobic flora was present in 10 (33%) patients. fifty isolates were recovered (1.7 per specimen); 27 were aerobic or facultative bacteria and 23 were strict anaerobes. the predominant aerobic and facultative bacteria were ...19957559963
in vitro activity of bay y 3118, and nine other antimicrobial agents against anaerobic bacteria.the antibacterial activity of bay y 3118, a new chlorofluoroquinolone, was determined against 257 strains of anaerobic bacteria and compared with the activities of ofloxacin, ciprofloxacin, sparfloxacin, imipenem, cefoxitin, clindamycin, chloramphenicol, metronidazole, and ornidazole. overall, bay y 3118 was the most active agent tested against the bacteroides fragilis group. its activity (mic90, 0.5 mg/l) was 16-fold lower than that of sparfloxacin (mic90, 8 mg/l), and more than 100-fold lower ...19957562012
[in vitro activity of carbapenems (biapenem, imipenem and meropenem) and some other antibiotics against strict anaerobic bacteria].during 1994, the in vitro antibiotic susceptibility of 306 anaerobic bacteria was performed in 4 hospitals, by the reference agar dilution method. among the 129 b. fragilis group strains, only two b. fragilis strains were resistant to the three carbapenems and all beta-lactams, even combined with beta-lactamase-inhibitors while metronidazole resistance could not be detected. evolution in antibiotic resistance rates could be assessed only for piperacillin whose resistance rates increased to 20%. ...19957567117
changes in the core tonsillar bacteriology of recurrent tonsillitis: 1977-1993.microbiological studies of the core of tonsils removed from children with recurrent tonsillitis due to group a beta-hemolytic streptococci were conducted during three periods, with 50 patients in each period: 1977-1978 (period 1), 1984-1985 (period 2), and 1992-1993 (period 3). mixed flora were present in all tonsils, with 8.1 organisms per tonsil (3.8 aerobes and 4.3 anaerobes). the predominant isolates in each period were staphylococcus aureus, moraxella catarrhalis, peptostreptococcus species ...19957578726
recombinant proteins l and lg: efficient tools for purification of murine immunoglobulin g order to improve antibody purification methods, recombinant proteins l and lg were tested in the purification of murine monoclonal immunoglobulin g (igg) and its fragments. after affinity constant evaluation in different buffer systems, high-performance affinity chromatographic columns were prepared by coupling the proteins to affi-prep 10 resin and tested with eight different murine monoclonal antibodies and their fragments of different isotypes. affinity chromatographic experiments confirme ...19957581856
the role of cefotaxime in the treatment of surgical infections.this study examines the role of cefotaxime in the treatment of both gram-negative and gram-positive surgical infections. a dose of 2 g of cefotaxime will sustain peripheral compartment concentrations of 2.6, 3.9, 1.6, and 0.7 micrograms/ml for 6, 8, 10 and 12 h, respectively. therefore, the proportion of pathogens with a minimal inhibitory concentration (mic) below the peripheral compartment cefotaxime concentrations was assessed as a measure of therapeutic potential. it was observed that bacter ...19957587036
the ga module, a mobile albumin-binding bacterial domain, adopts a three-helix-bundle structure.we present the first study of the secondary structure and global fold of an albumin-binding domain. our data show that the ga module from protein pab, an albumin-binding protein from the anaerobic bacterial species peptostreptococcus magnus, is composed of a left-handed three-helix bundle. the helical regions were identified by sequential and medium range noes, values of nh-c alpha h coupling constants, chemical shift indices, and the presence of slowly exchanging amide protons, as determined by ...19957589548
role of escherichia coli p fimbriae in intestinal colonization in gnotobiotic rats.adherence via p fimbriae is associated with long-term persistence of escherichia coli in the human large intestine, but a causal relationship has not been proven. in the present study, germfree rats were colonized with a mixture of two isogenic e. coli strains, one p fimbriated and the other type 1 fimbriated. both types of fimbriae conferred adherence to rat colonic epithelial cells. with two mutant strains from a pyelonephritogenic isolate of serotype o75:k5:h-, the p-fimbriated strain 824 att ...19957591136
an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro.there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ...19957593703
factors affecting the occurrence of bacteremia associated with tooth extraction.this study examined the factors that affect the occurrence of bacteremia associated with tooth extraction and the kinds of bacteria causing this bacteremia. bacteremia was found in 132 (72.1%) of 183 patients who had one or more teeth extracted for various reasons. bacteremia occurred more frequently when teeth were extracted because of inflammatory dental diseases. the occurrence of bacteremia also increased with the number of teeth extracted and the age of the patients. when the volume of bloo ...19957594761
antibacterial effects of some root end filling addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. this investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, super eba and a mineral trioxide aggregate on nine facultative bacteria streptococcus fecalis, streptococcus mitis, streptococcus mutans, streptococcus salivarius, lactobacillus species, staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, and esc ...19957595152
mapping of the immunoglobulin light chain-binding site of protein l.protein l is a cell surface protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule binds specifically and with high affinity to immunoglobulins (ig) of a wide range of animal species. the ig-binding activity is mediated through five highly homologous domains, each 72 to 76 amino acid residues long, which interact with framework regions in the variable domain of ig light chains. the interaction does not interfere with the antigen binding capac ...19957608965
clinical features and aerobic and anaerobic microbiological characteristics of investigate the aerobic and anaerobic microbiological characteristics of cellulitis and correlate them with the infection site.19957611872
microbiologic and clinical study of polytetrafluoroethylene membranes for guided bone regeneration around implants.this study determined the microbiota of the mucosa- and implant-facing parts of expanded polytetrafluoro ethylene augmentation material, and the influence of major periodontopathogens on the healing process associated with guided bone regeneration around dental implants. seventeen patients with nine dehiscence and eight extraction defects were studied. prior to surgery and at membrane removal, microbial morphotypes, total viable counts, and the occurrence of selected microbial species were exami ...19957615319
prosthetic-valve endocarditis caused by peptostreptococcus anaerobius. 19957620041
periodontal microbiota of mobile and non-mobile teeth.the mechanism of accelerated periodontal destruction around teeth with occlusal trauma and increased mobility remains unclear. one possibility is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. this study compared the subgingival microflora in mobile and non-mobile teeth of 35 adults on supportive maintenance therapy and 15 with untreated adult periodontitis. in each subject, subgingival paper-point samples were obtained from a mobile tooth ...19957623258
[nosocomial infections in present-day traumatological-orthopedic hospitals].nosocomial infection remains a significant problem in modern traumatology and orthopedics. staphylococcus prevails in its etiological pattern. the activation of microorganisms such as acinetobacter is noted. in addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. anaerobic infection is seedy in 30.4% of patients' blood samples. great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. the authors' ...19957626997
a numerical taxonomic study of the gram-positive anaerobic cocci.clinical (101) and collection (26) strains of gram-positive anaerobic cocci were examined in conventional tests and pyrolysis mass spectrometry (pms). numerical classifications based upon conventional test reaction patterns (ctrps) and pms showed 27 and 22 clusters, respectively. cross-tabulation of cluster membership in the two classifications showed excellent correlation, with the combined classifications showing clear groups corresponding to the currently recognised species peptostreptococcus ...19957629855
[antibacterial activities of cefmenoxime against recent fresh clinical isolates from patients in sinusitis].in order to evaluate antimicrobial activity of cefmenoxime (cmx), minimum inhibitory concentrations (mics) of cmx and control drugs were determined against clinical isolates from patients of sinusitis that were obtained in our laboratory from october of 1993 to march of 1994. the results are summarized as follows; 1. cmx showed strong antimicrobial activities against streptococcus pneumoniae, haemophilus influenzae and moraxella subgenus branhamella catarrhalis that were 3 major aerobic bacteria ...19957637194
liver abscesses as initial presentation of crohn's disease. 19957639260
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