PMID(sorted descending)
contrasting roles for symmetrically disposed beta-turns in the folding of a small investigate the role of turns in protein folding, we have characterized the effects of combinatorial and site-directed mutations in the two beta-turns of peptostreptococcal protein l on folding thermodynamics and kinetics. sequences of folded variants recovered from combinatorial libraries using a phase display selection method were considerably more variable in the second turn than in the first turn. these combinatorial mutants as well as strategically placed point mutants in the two turns h ...19979417937
[microflora of periodontal pockets in advanced periodontitis].the aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. from each subject 16-20 samples were taken using paper points. pooled sample after 60 s. mixing was serially diluted in reduced bhi. for total cell counts and for the isolation of black pigmented anaerobes brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without kanamycin-vancomycin mixture and bm agar plates were used. for isolation of a. actinomycetemcomitans ...19979411079
[aerobic and anaerobic bacterial flora in chronic sinusitis in adults].the aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). the isolated aerobic bacteria were as follows: streptococci from the species streptococcus salivarius, streptococcus anginosus, streptococcus group c, streptococcus sanguis, ...19979411078
[anti-anaerobic activity of carbapenems].the critical concentrations of sensitivity and resistance for meropenem versus anaerobic bacterias were analyzed. it is demonstrated the meropenem is a powerful inhibitor of the microorganisms of the bacteroides groups (mic90 < 1 mg/l), prevotella > porphyromonas, fusobacterium, veillonella, clostridium perfringens is inhibited at a mic < 0.06 mg/l. the mic of meropenem versus c. difficile is 2 mg/l. they are also highly susceptible to the propionibacterium, peptostreptococcus and peptococcus ty ...19979410076
[meropenem: microbiologic perspective].meropenem is a beta-lactamic carbapenem derived from thienamycin and is structurally characterized by the presence of a beta-methyl group in position c1 which confers stability to the molecule versus renal dehydropeptidase 1 (dhp-1), thereby making the coadministration of an enzyme inhibitor unnecessary. its esterochemical configuration of the lateral chain in c2 (dimethyl carbomoilpyrrolidenethium) increases the activity versus gram negative bacteria (enterobacteria and pseudomonas) and moreove ...19979410064
macrolides resistance of common bacteria isolated from determine the susceptibility to macrolides of common pathogenic bacteria isolated from taiwan, the in vitro activities of erythromycin, roxithromycin, azithromycin, clarithromycin, and dirithromycin were tested against 492 clinical isolates of eight different bacteria, collected from the national taiwan university hospital. the results showed high minimum inhibitory concentrations (mics) against most of the tested bacteria. the mic90s for staphylococcus aureus (both methicillin-resistant and ...19959407220
evaluation of the bbl crystal anaerobe identification system.the bbl crystal anaerobe (anr) identification system was evaluated, and the results were compared with those from conventional anaerobic methods. we tested 322 clinically significant anaerobic bacteria according to the manufacturer's instructions. the system identified correctly 286 of 322 (88.8%) of the anaerobic bacteria tested. of these, 263 of 322 (81.7%) were identified correctly on initial testing and 49 were identified correctly only to the genus level; on repeat testing, 23 of 49 (46.9%) ...19979399517
[trend of bacterial meningitis in children over a 14 year period (1981 through 1994) in japan--an analysis based on studies in 27 institutions].we observed 266 children with purulent meningitis in 27 institutions in japan during the 14 years from 1981 on dividing these years into 3 periods, 1981-1985, 1986-1990 and 1991-1994, and studied the trend of causative organisms identified in 254 among the 266 patients. their ages were less than 3 months after birth in 50 children and 3 months or older in 216: there were 141 boys and 125 girls. the causative organisms were h. influenzae in 134 patients and s. pneumoniae in 50, most of them being ...19979394553
[antimicrobial activities of clarithromycin against recent obtained clinical isolates].in order to evaluate antimicrobial activities of clarithromycin (cam), minimum inhibitory concentrations (mics) of cam and control drugs were determined against clinical isolates that were obtained from outpatients in 1994 and 1996. the results are summarized as follows; 1. it was not showed that cam-resistant strains were increasing among staphylococcus spp., beta-streptococci, moraxella subgenus branhamella catarrhalis, haemophilus influenzae, bordetella pertussis, campylobacter jejuni subsp. ...19979394238
[site-specific microbiological tests and the diagnostic significance-- dental and oral infections]. 19979379527
characterization of the free energy spectrum of peptostreptococcal protein l.native state hydrogen/deuterium exchange studies on cytochrome c and rnase h revealed the presence of excited states with partially formed native structure. we set out to determine whether such excited states are populated for a very small and simple protein, the igg-binding domain of peptostreptococcal protein l.19979377710
coronal leakage of mixed anaerobic bacteria after obturation and post space preparation.the purpose of this study was to assess bacterial leakage of a mixed anaerobic community of organisms in obturated canals after post space preparation.19979377197
taxonomic update and clinical significance of species within the genus peptostreptococcus. 19979376474
metastatic bacterial endophthalmitis. a report of four cases all leading to draw attention to the rare but severe entity of endophthalmitis as encountered due to metastatic spread of bacteria.19979374263
distribution and mobility of the tetracycline resistance determinant tetq.we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ...19979372425
prospective study of bacteremia rate after elastic band ligation and sclerotherapy of esophageal varices in patients with hepatosplenic schistosomiasis.esophageal band ligation is considered to be as efficient as endoscopic sclerotherapy, with a lower complication rate, including bacteremia. there are few studies comparing the two methods. the aim of this study was to compare the incidence of bacteremia after both treatments in patients with portal hypertension secondary to schistosomiasis.19979351034
the aerobic and anaerobic bacteriology of perirectal abscesses.the microbiology of perirectal abscesses in 144 patients was studied. aerobic or facultative bacteria only were isolated in 13 (9%) instances, anaerobic bacteria only were isolated in 27 (19%) instances, and mixed aerobic and anaerobic flora were isolated in 104 (72%) instances. a total of 325 anaerobic and 131 aerobic or facultative isolates were recovered (2.2 anaerobic isolates and 0.9 aerobic isolates per specimen). the predominant anaerobes were as follows: bacteroides fragilis group (85 is ...19979350771
in-vitro activity of spiramycin and metronidazole alone or in combination against clinical isolates from odontogenic hundred and forty-eight isolates of bacteria from 20 intraoral odontogenic abscesses were tested for their susceptibility to spiramycin and metronidazole alone or in combination. all isolates, except rothia spp. (one), enterococcus avium (three), haemophilus parainfluenzae (one) and staphylococcus aureus (one) were sensitive to spiramycin and/or metronidazole. among the anaerobes, spiramycin as well as metronidazole showed good antimicrobial activity against species of prevotella, eubacteriu ...19979338486
bilateral anaerobic empyemas complicating infectious mononucleosis. 19979315821
dental implant implants provide a restorative tool to support crowns, bridge abutments, and removable dentures. osseointegrated implants are titanium posts that are surgically implanted in alveolar bone. a tight immobile bond (osseointegration) forms between bone and titanium, and prosthetic and restorative fixtures are attached to the implants. titanium implants differ from natural teeth, which may make them more susceptible to mechanical stress. a small proportion of implants are not successful and ma ...19979310682
recently described clinically important anaerobic bacteria: taxonomic aspects and update.a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ...19979310640
increased susceptibility of rag-2 scid mice to dissemination of endodontic infections.specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. to investigate this question we compared periapical-lesion pathogenesis in rag-2 severe combined immunodeficient (scid) mice with immunocompetent control mice following surgical pulp exposure. in order to equalize the bacterial challenge, an infection protocol ...19979284152
peptostreptococcal myonecrosis of extraperitoneal origin--a life-threatening complication of pelvic ring disruption.this report illustrates the clinical and pathological findings of anaerobic streptococcal myonecrosis following extraperitoneal rectal injury in a 28-year-old patient with traumatic pelvic ring disruption. four days following admission, the patient underwent laparotomy, debridement and drainage of the left-sided abdominal wall and presacral space for gross surgical emphysema and streptococcal anaerobic myonecrosis. the specificity of this infection and its impact on the diagnosis and treatment o ...19979283248
use of the isolator 1.5 microbial tube for culture of synovial fluid from patients with septic arthritis.synovial fluid specimens obtained from patients with arthritis were plated onto solid media (conventional cultures) or inoculated into an isolator 1.5 microbial tube (isolator cultures), and the yield and time to detection of organisms were compared. overall, 144 specimens obtained from 137 patients were processed, and 31 (21.5%) cultures obtained from 29 patients were positive by at least one method. staphylococcus aureus was isolated from 12 patients, streptococcus pneumoniae and kingella king ...19979276427
effect of the addition of peptostreptococcus productus atcc35244 on the gastro-intestinal microbiota and its activity, as simulated in an in vitro simulator of the human gastro-intestinal tract.peptostreptococcus productus atcc35244, a reductive acetogenic strain, was added daily over 9 successive days to the fourth vessel (ascending colon) of the shime, a six-stage reactor system simulating the in vivo continuous culture conditions of the human gastro-intestinal tract. final numbers of organisms (cfu)/ml reactor contents (c) were attained such that log10c = 6.9 +/- 0.1. the addition caused the ch4 production to decrease below the detection limit while total gas and co2 production in t ...19979274052
beta-lactamase producing bacteria in adult 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ...19979266340
microorganisms in vaginal fluid from women in prolonged order to compare the vaginal microflora of women in prolonged pregnancy with that of women who delivered at term, samples for quantitative aerobic and anaerobic microbiological culture were collected from 100 women at 42 weeks of gestation and from 60 women at term. the occurrence of lactobacilli-dominated flora was similar in women at term and women with prolonged pregnancy. however, non-hydrogen-peroxide-producing lactobacilli (p < 0.01) were significantly more common and peptostreptococci ...19979251948
separation method of igg fragments using protein l.protein l (igg kappa-chain-binding bacterial protein) showed a precipitate line(pseudo-immuno-reaction) with igg and f(ab')2 fragment, but did not show any line with the fab fragment, the fc fragment and free kappa-chains in the micro-ouchterlony method. the igg and fab fraction obtained from pa-pain-digested igg (from the sera of patients with chronic thyroiditis), followed by protein a-sepharose, were separated by protein l-sepharose affinity chromatography. the unbound fraction (uf) consisted ...19979246561
cloning and expression of the two genes coding for l-serine dehydratase from peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both l-serine dehydratases from escherichia coli.the structural genes sdha and sdhb, coding for the alpha- and beta-subunits of the [4fe-4s] cluster containing l-serine dehydratase from peptostreptococcus asaccharolyticus, have been cloned and sequenced. expression of modified sdhb together with sdha in escherichia coli led to overproduction of active his6-tagged l-serine dehydratase. e. coli mew22, deficient in the l-serine dehydratase l-sd1, was complemented by this sdhba construct. the derived amino acid sequence of sdhba shares similaritie ...19979244285
ovarian vein thrombosis with symptoms prior to term--a case report.a case with ovarian vein thrombosis before delivery is presented. c-reactive protein exceeded 200 mg/l, and the patient was treated for a suspected infection before the true diagnosis was revealed by ct. after aggressive anti-coagulant therapy, a follow-up ct showed complete remission.19979243212
nmr analysis of the interaction between protein l and ig light chains.protein l is a cell wall protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. it binds to immunoglobulin (ig) light chains predominantly of the kappa subtype from a wide range of animal species. this binding is mediated by five highly homologous repeats designated as b1-b5, each of which comprises 72 to 76 amino acid residues. the fold of the ig light chain-binding b1 domain of protein l has previously been shown to comprise an alpha-helix packed agains ...19979231896
occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings.bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. the purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. dentin ...19979226388
time-kill study of the activity of trovafloxacin compared with ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against six anaerobes.a time-kill method was developed to examine the killing kinetics of trovafloxacin, ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against one strain each of bacteroides fragilis, bacteroides thetaiotaomicron, prevotella melaninogenica, fusobacterium mortiferum, peptostreptococcus magnus and clostridium perfringens. solutions and suspensions were prepared inside an anaerobic glove box, using syringes and prereduced broth. bottles were then incubate ...19979222066
immune response to fusobacterium nucleatum, prevotella intermedia and other anaerobes in children with acute tonsillitis.the number of aerobic and anaerobic bacteria was determined in the saliva of 20 children with acute group a beta-haemolytic streptococcal (gabhs) pharyngo-tonsillitis, and 20 with acute non-gabhs tonsillitis. antibody titres to four gram-negative anaerobic bacilli that reside in the oropharynx (fusobacterium nucleatum, prevotella intermedia, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans) were determined in these and 20 control patients. an average of 8.8 aerobic and anaerobi ...19979222046
the antimicrobial activity of endodontic sealers to anaerobic bacteria.the effect of five root canal sealers and two root canal dressing materials on the growth of three anaerobic bacteria associated with endodontic infections was determined using the agar diffusion inhibitory test. samples of the following endodontic sealers (apexit, ketac-endo, roth sealer, sealapex and tubliseal) and root canal dressing materials (pulpdent and root-cal) were incubated for 48 h with the following anaerobic bacteria: capnocytophaga ochracea, porphyromonas gingivalis and peptostrep ...19969206446
variations in the susceptibilities of components of the endodontic microflora to biomechanical procedures.the role of the endodontic microflora in pulpal disease and in endodontic treatment failures is well established. thus the need for effective microbial control is one of the important justifications for biomechanical procedures. however the efficacy of this stage of treatment is dependent upon the vulnerability of the involved species, which may not be uniform. the aim of this study was to investigate variations in the susceptibilities of members of the root canal microflora to routine biomechan ...19969206439
associations of endodontic symptoms and signs with particular combinations of specific bacteria.significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ...19969206427
microbiology of nonbullous impetigo.our objective was to establish the aerobic and anaerobic microbiology of nonbullous impetigo (ni) in children. we used a retrospective review of clinical microbiology laboratory and patients' records. specimens were obtained from 40 patients with ni lesions and showed bacterial growth. aerobic or facultative anaerobic bacteria only were present in 24 patients (60%), strict anaerobic bacteria only in 5 patients (12.5%), and mixed anaerobic-aerobic flora was present in 11 patients (27.5%). sixty-f ...19979192410
periodontal findings in cohen syndrome with chronic neutropenia.radiographic periodontal status and microbiological findings of periodontal pockets in subjects with cohen syndrome are presented in this report. this hereditary disorder causes mental retardation, and neutropenia is one feature of the syndrome. fifteen patients with cohen syndrome and 15 controls matched for age and sex and, as far as possible, according to the degree of mental retardation were examined. alveolar bone loss was evaluated from the panoramic radiographs. two subgingival samples we ...19979182743
protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum.the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ...19979178115
matrix metalloproteinases-1, -3 and -8 and myeloperoxidase in saliva of patients with human immunodeficiency virus infection.human immunodeficiency virus (hiv)-seropositive patients have frequently severe gingival inflammation and/or attachment loss. in addition many infectious diseases affect their periodontium with varying clinical manifestations. matrix metalloproteinases seem to play a key role in physiological periodontal remodelling and pathological tissue destruction. the aim of the present study was to characterize the presence, molecular forms, cellular sources, activities, and relative amounts of fibroblast- ...19969171509
organisms isolated from dogs and cats with anaerobic infections and susceptibility to selected antimicrobial determine the prevalence of obligate anaerobic bacteria in bacterial infections in dogs and cats and susceptibility to selected antimicrobial agents.19979170087
microbiology of chronic maxillary sinusitis: comparison between specimens obtained by sinus endoscopy and by surgical drainage.the aerobic and anaerobic microbiology of sinus aspirates obtained during surgery was compared with culture of samples obtained by endoscopy. six patients with chronic maxillary sinusitis were evaluated. polymicrobial flora was found in all specimens (two-to-five isolates/sample). a total of 24 isolates (18 anaerobic, five aerobic and one micro-aerophilic) was obtained from sinus aspirates, and 25 isolates (16 anaerobic and nine aerobic) were found in endoscopic specimens. the predominant organi ...19979152041
synthesis by an alpha-glucosidase of glycosyl-trehaloses with an isomaltosyl residue.glycosyl-trehaloses with an isomaltosyl residue were synthesized by alpha-glucosidase from aspergillus niger by using maltotetraose as a glucosyl donor and trehalose as the acceptor. the one trisaccharide and two tetrasaccharides formed were isolated by successive column chromatography. the results of an enzymatic digestion, methylation analysis, and 13c-nmr studies indicated that these oligosaccharides were alpha-isomaltosyl alpha-glucoside, alpha-isomaltotriosyl alpha-glucoside and alpha-isoma ...19979145529
antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects.tosufloxacin, a quinolone, showed a broad antibacterial spectrum against gram-positive and gram-negative bacteria including anaerobic bacteria. tosufloxacin was 4- to 8-fold more active than levofloxacin and ciprofloxacin. the mic90 of tosufloxacin for clinical isolates of bacteroides fragilis, bacteroides vulgatus, bacteroides thetaiotaomicron and peptostreptococcus asaccharolyticus were 0.78, 0.39, 1.56 and 0.39 micrograms/ml, respectively. morphological observation with the scanning and trans ...19979142454
a case of secondary sjögren's syndrome with a swollen thigh. 19979135217
penicillin g and penicillin g-tinidazole treatment of experimentally induced summer mastitis--effect on elimination rates of bacteria and outcome of the disease.two udder hind quarters of ten pregnant heifers were inoculated experimentally with a combination of actinomyces pyogenes, peptostreptococcus indolicus and fusobacterium necrophorum at a dose of 9 x 10(8) colony forming units (cfu) of each species of bacteria. the development of aerobic-anaerobic mastitis was followed-up clinically and through analysis of udder secretion samples. all heifers developed acute clinical mastitis. thick, purulent secretions with foul odour were already present 24 h a ...19949133056
tissue penetration of meropenem in patients undergoing gynecologic surgery.the purpose of this study was to assess the tissue-penetrating ability of a new beta-lactam antibiotic, meropenem, in 64 patients undergoing elective gynecologic surgery. patients received a single 500-mg dose intravenously before surgery. plasma and tissue concentrations of meropenem were highest at approximately 1 hour, and good tissue penetration was seen in the variety of specimens evaluated. the median plasma concentration at approximately 1 hour was 13.3 micrograms/ml. the median fluid and ...19979126690
kinetics of folding of the igg binding domain of peptostreptococcal protein l.the kinetics of folding of a tryptophan containing mutant of the igg binding domain of protein l were characterized using stopped-flow circular dichroism, stopped-flow fluorescence, and hd exchange coupled with high-resolution mass spectrometry. both the thermodynamics and kinetics of folding fit well to a simple two-state model: (1) guanidine induced equilibrium denaturation transitions measured by fluorescence and circular dichroism were virtually superimposable. (2) the kinetics of folding/un ...19979116017
polymicrobial brain abscess in a patient infected with human immunodeficiency virus.although intracranial mass lesions that occur as a result of infection have commonly been reported in patients infected with human immunodeficiency virus, polymicrobial pyogenic brain abscess has not been described in this setting. we report the first case of a patient with a polymicrobial brain abscess involving streptococcus bovis, fusobacterium necrophorum, peptostreptococcus, and group g streptococcus, and we review the relevant world literature.19979114198
cervical necrotizing fasciitis as a complication of acute epiglottitis. 19979106089
characterization of smooth and rough morphotypes of peptostreptococcus micros.isolation of the smooth (sm) morphotype of peptostreptococcus micros, a suspected oral pathogen, is sometimes accompanied by isolation of a rough (rg) morphotype of p. micros. the rg type readily changes to a sm-like variant (rgsm) in broth culture. sm and rg isolates and rgsm variants were compared to determine whether these three types are the result of phase variation. the rgsm variants resembled the sm morphotype in colony morphology; furthermore, the sm type and the rgsm type did not have t ...19979103622
[the detection of peptostreptococcus asaccharolyticus and bacillus cereus in clinical materials from a child who died of sepsis with a fulminant clinical course].a case of an acute disease with a rapid clinical course and a fatal outcome in the presence of irreversible toxicoinfectious shock, appearing in two children after the consumption of sheep kidneys, is described. the post mortem examination of the children revealed the presence of hemorrhagic, erosive and necrotic areas on the mucous membrane of the stomach, the duodenum and the upper section of the small intestine. from the material obtained by probing the stomach of one of the children 6 hours ...19969103069
periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants.the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ...19979100195
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between july 1995 and june 1996].isolated bacteria from infections in general surgery during the period from july 1994 to june 1995 were investigated in a multicenter study in japan, and the following results were obtained. one hundred and sixty-four strains were isolated from primary infections, and 202 strains were isolated from postoperative infections. from primary infections, anaerobic gram-positive bacteria were predominant, while from post operative infections, aerobic gram-positive bacteria were predominant. among aerob ...19979100076
a comparison of susceptibility results of the bacteroides fragilis group and other anaerobes by traditional mic results and statistical methods.antimicrobial susceptibility is compared by mic values reflecting activity by weight and/or percentage of strains resistant. this study establishes these parameters in vitro in the traditional fashion and extends analysis statistically for various groups of anaerobic bacteria. mode mic, mic50, mic90 values and percentage of strains resistant indicated comparable activity for most of the beta-lactams. geometric and arithmetic mics suggested ceftizoxime to be the most active beta-lactam. statistic ...19979096180
solution structure of the albumin-binding ga module: a versatile bacterial protein domain.the albumin-binding ga module is found in a family of surface proteins of different bacterial species. it comprises 45 amino acid residues and represents the first known example of contemporary module shuffling. using 1h nmr spectroscopy we have determined the solution structure of the ga module from protein pab, a protein of the anaerobic human commensal and pathogen peptostreptococcus magnus. this structure, the first three-dimensional structure of an albumin-binding protein domain described, ...19979086265
septic arthritis after arthroscopic anterior cruciate ligament reconstruction. diagnosis and management.we performed a retrospective study of knee joint infections after arthroscopic anterior cruciate ligament reconstruction at our institution. two thousand five hundred anterior cruciate ligament reconstructions were performed between 1988 and 1993. seven (0.3%) patients experienced postoperative deep infections of the knee. all anterior cruciate ligament reconstructions were performed using arthroscopically assisted techniques. six (86%) of these patients had concomitant open procedures performed ...19979079185
inhibitory effects of metronidazole on anaerobic metabolism of phenylalanine and leucine by peptostreptococcus anaerobius.the metabolism of phenylalanine and leucine by peptostreptococcus anaerobius and the effects of metronidazole on this metabolism were studied. the cells metabolized both phenylalanine and leucine under strict anaerobic conditions and produced hydroxylated products (i.e. phenyllactate from phenylalanine and hydroxyisocaproate from leucine). other products were phenylpropionate, cinnamate and phenylacetate from phenylalanine; isocaproate, alpha-ketoisocaproate and isovalerate from leucine. metroni ...19979069531
anaerobic cocci of clinical importance.the current knowledge is reviewed concerning the anaerobic cocci, in particular those of clinical relevance. the anaerobic cocci are defined and their current taxonomic positions discussed. it is clear that new genera and species await to be characterised fully. the overwhelming majority found in clinical material belong to the genus peptostreptococcus, with the remainder belonging to the veillonellae and, possibly, ruminococci. human infections with other anaerobic cocci are extremely rare. the ...19969069108
aerobic and anaerobic microbiology of bacterial tracheitis in children.establish the aerobic and anaerobic microbiology of bacterial tracheitis in children.19979061728
equilibrium and pre-equilibrium fluorescence studies on the interaction between protein l and kappa light chain. 19979056936
the clinical, microbial, and host response characteristics of the failing implant.the goal of this study was to provide new data regarding levels of inflammatory and growth factor mediators and bacterial pathogens associated with failing implants, as compared to healthy implants. twenty-one patients with failing implant sites (group 1) and 8 patients with only healthy implants (group 2) were included. fifteen of the 21 failing implant patients (group 1) also presented with at least one stable nondiseased implant. plaque samples were examined, using dna oligonucleotide probes ...19979048452
evaluation of the anaeropack system for growth of clinically significant anaerobes.the anaeropack (mitsubishi gas chemical america, inc., new york, n.y.) system was compared with the gaspak (becton dickinson microbiology systems, cockeysville, md.) system and a conventional anaerobe chamber to evaluate the ability of the anaeropack system to support the growth of clinically significant anaerobes. the anaeropack system requires no catalyst or water, produces no hydrogen, and is oxygen absorbing and carbon dioxide generating. it is simple to use and reduces preparation time to a ...19979041388
development and evaluation of a selective and differential medium for the primary isolation of peptostreptococcus micros.peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. a selective and differential medium for the primary isolation of p. micros was developed and evaluated. columbia cna agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (p. micros medium: pmm). p. micros has a chara ...19969028263
electrophoresis of whole-cell soluble proteins of microorganisms isolated from bacteremias in endodontic therapy.we have previously demonstrated that anaerobic bacteria are the microorganisms most frequently isolated from blood following endodontic therapy of teeth with apical periodontitis. phenotypic characterisation of the isolates suggested that the bacteria in the blood originated from the root canal. the present experiment using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was carried out in an effort to verify these findings, and to further study the microorganisms involved i ...19969021323
microbiology of otitis externa.microbiologic and clinical data from 26 patients with otitis externa were prospectively evaluated. specimens were processed for aerobic and anaerobic bacteria. bacterial growth was noted in 23 specimens. a total of 33 aerobic and 2 anaerobic bacteria were recovered. aerobic bacteria only were isolated in 21 (91%) patients, anaerobic bacteria only in 1 (4%), and mixed aerobic and anaerobic bacteria in 1 (4%). the most common isolates were pseudomonas aeruginosa (14 instances), staphylococcus aure ...19979018252
contemporary management of deep neck space infections.deep neck infections continue to be seen despite the wide use of antibiotics. these infections follow along fascial planes to create deep neck space abscesses. the clinical presentation often points to the space involved. understanding the regional anatomy gives the surgeon the ability to treat these grave infections. the records of 24 patients with a diagnosis of deep neck space abscess admitted to hermann hospital between 1988 and 1993 were reviewed. fifty percent of the patients had received ...19979018251
conformational analysis of peptides corresponding to all the secondary structure elements of protein l b1 domain: secondary structure propensities are not conserved in proteins with the same fold.the solution conformation of three peptides corresponding to the two beta-hairpins and the alpha-helix of the protein l b1 domain have been analyzed by circular dichroism (cd) and nuclear magnetic resonance spectroscopy (nmr). in aqueous solution, the three peptides show low populations of native and non-native locally folded structures, but no well-defined hairpin or helix structures are formed. in 30% aqueous trifluoroethanol (tfe), the peptide corresponding to the alpha-helix adopts a high po ...19979007989
[antimicrobial activity of cefodizime against clinical isolates].in order to evaluate antimicrobial activity of cefodizime (cdzm), minimum inhibitory concentrations (mics) of cdzm and control drugs were determined against clinical isolates collected from nation-wide medical institutions and in our laboratory from september to december of 1992 and from september to december of 1995. the results are summarized as follows: 1. bacterial species with no or few strains resistant to cdzm included streptococcus pyogenes, haemophilus influenzae, citrobacter koseri, pr ...19968986558
effect of 2-bromoethanesulfonic acid and peptostreptococcus productus atcc 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis.evidence is provided that reductive acetogenesis can be stimulated in ruminal samples during short-term (24-h) incubations when methanogenesis is inhibited selectively. while addition of the reductive acetogen peptostreptococcus productus atcc 35244 alone had no significant influence on ch4 and volatile fatty acid (vfa) production in ruminal samples, the addition of this strain together with 2-bromoethanesulfonic acid (bes) (final concentration, 0.01 or 0.03 mm) resulted in stimulation of acetic ...19978979351
an rrna approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination.ruminal amino acid degradation is a nutritionally wasteful process that produces excess ruminal ammonia. monensin inhibited the growth of monensin-sensitive, obligate amino acid-fermenting bacteria and decreased the ruminal ammonia concentrations of cattle. 16s rrna probes indicated that monensin inhibited the growth of peptostreptococcus anaerobius and clostridium sticklandii in the rumen. clostridium aminophilum was monensin sensitive in vitro, but c. aminophilum persisted in the rumen after m ...19968975611
[role of 67 gallium citrate and 99mtc-hmpao-labelled neutrophils in the integrated diagnostic imaging in a case of peptostreptococcus anaerobius hepatic abscess]. 19968966261
[successful scartectomy and cryoablation for ventricular tachycardia occurring late after correction of tetralogy of fallot].a 26-year-old man had a total repair of tetralogy of fallot at 1 year of age, and had redo surgery for restenosis of the right ventricular outflow tract and small residual vsd at 11 years of age. after the second operation, av block developed and an endocardial pacemaker system was implanted. for the last 3 years, he had mild febrile episodes, cough, occasional hemoptysis and paroxysmal ventricular tachycardia. because of his refractory tachycardia and suspected infective endocarditis, he was ad ...19968964997
degradation of lactoferrin by periodontitis-associated bacteria.the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...19968961558
the adherence of periodontopathogens to periodontal probes. a possible factor in intra-oral transmission?periodontal probes have previously been shown to harbor several bacterial types or species after probing periodontally diseased pockets. this study aims to identify and quantify periodontopathogens that may adhere to a periodontal probe by culturing techniques. it also examines the probe's roughness on its capability to collect bacteria, comparing merrit-b probes (with deep indentations) with tps probes (with smooth surfaces). from the differential phase contrast microscopy findings it was seen ...19968959565
emerging resistance of anaerobic bacteria to antimicrobial agents in south previous studies, bacteroides fragilis group organisms isolated from korean patients were more frequently resistant to various antimicrobial agents, including clindamycin, than were isolates in other countries. a recent report of increased resistance of peptostreptococcus species prompted us to include such isolates in a study of antimicrobial susceptibility. anaerobes isolated in 1994 at a tertiary care hospital in seoul were tested by agar dilution method. none of the b. fragilis group orga ...19968953110
bacteriology of dentoalveolar abscesses in patients who have received empirical antibiotic therapy.we investigated aspirates of pus from dentoalveolar abscesses in 13 patients who had received empirical antibiotic therapy within 1 month before presentation at our facility. the antibiotics administered were lincomycin, amoxicillin, ampicillin, or a cephalosporin. information on antibiotic therapy was obtained from the patients' medical histories. cultures of all dentoalveolar abscesses yielded organisms. a total of 70 isolates (42 anaerobes and 28 facultative organisms) were recovered, represe ...19968953107
comparison of activities of trovafloxacin (cp-99,219) and five other agents against 585 anaerobes with use of three media.agar dilution testing was used to compare the in vitro activities of trovafloxacin and ofloxacin, ciprofloxacin, imipenem, metronidazole, and clindamycin against 585 anaerobes. the activity of trovafloxacin was superior to those of ofloxacin and ciprofloxacin, with 94%, 91%, 96%, 100%, 90%, and 100% inhibition of bacteroides fragilis, non-fragilis bacteroides species, peptostreptococcus magnus/peptostreptococcus micros, clostridium perfringens, prevotella bivia/prevotella disiens, and fusobacter ...19968953106
in vitro susceptibility of anaerobes to quinolones in the united states.the in vitro activity of early fluoroquinolone antibodies--including ciprofloxacin, ofloxacin, fleroxacin, pefloxacin, enoxacin, and lomefloxacin--against most anaerobes has been limited, a characteristic making them poor choices as antianaerobic agents. newer fluoroquinolones, including levofloxacin, sparfloxacin, and grepafloxacin, have moderate activity against anaerobes, including the bacteroides fragilis group as well as clostridium, peptostreptococcus, prevotella, and fusobacterium species ...19968953099
the vaginal microflora of pig-tailed macaques and the effects of chlorhexidine and benzalkonium on this characterize normal vaginal microflora of pig-tailed macaques and to evaluate two commonly used intravaginal compounds, chlorhexidine (chg), a vaginal antiseptic (surgilube, e. fougera, melville, ny), and benzalkonium chloride (bzk) (spermicidal contraceptive) in this monkey model to assess effects on the vaginal microflora.19968946634
degradation of host protease inhibitors and activation of plasminogen by proteolytic enzymes from porphyromonas gingivalis and treponema denticola.bacterial proteases may participate in the pathogenesis of periodontal diseases through their action on host proteins. in the present study, the ability of selected periodontopathogens, as well as two proteases isolated from porphyromonas gingivalis and treponema denticola, to degrade host protease inhibitors was evaluated. the activation of human plasminogen by the two bacterial proteases was also investigated. proteolytic breakdown of host protease inhibitors (alpha-1-antitrypsin, antichymotry ...19968936322
[antimicrobial activities of clarithromycin against clinical isolates].to examine the antimicrobial activity of clarithromycin (cam) against strains clinically isolated from outpatients in 1994, minimum inhibitory concentrations (mics) were determined for cam and the control drugs. the results were as follows; 1. mic50 and mic90 of cam were similar to those investigated in 1980's against many bacterial species. 2. cam showed strong antimicrobial activities against beta-lactamase producing moraxella subgenus branhamella catarrhalis, bordetella pertussis, campylobact ...19968935125
[the study of drug resistance in aerobic and anaerobic bacterial flora to selected antibacterial drugs].the aim of the study to determine the resistance to certain antibacterial drugs of the aerobic and anaerobic bacterial flora isolated from patients operated on for acute abdominal infections. the resistance was investigated by the disc diffusion method. among the isolated aerobes enterobacteriaceae, enterococcus spp, pseudomonas spp. staphylococcus spp were most frequent, and in the group of anaerobes the most frequent were bacteroides spp, peptostreptococcus spp and peptococcus spp. the sensiti ...19968926770
[occurrence of non-spore forming anaerobic bacteria in the upper airways of patients with chronic obstructive pulmonary disease].the aim of this study was to assess the occurrence of anaerobic non-sporeforming bacteria upper airways flora in the exacerbation of copd. sputum from 35 copd patients was sent to the laboratory in sterile, filled with co2 containers and cultured under anaerobic conditions. in all patients bacteriological tests were positive. the most common anaerobic bacteria were as follows: peptostreptococcus - in 28 pts, fusobacterium - in 27 pts, bacteroides - in 26 pts and prevotella in 24. less common wer ...19968926767
[orbit complication of sinusitis. a report of 7 cases].orbital complications of sinusitis were reviewed. seven cases seen in a 6-year period from 1989 to 1994 were classified following chandler. the pathogenesis, classification, diagnosis, and treatment of orbital complications, which are rare and steadily decreasing in frequency, are discussed. because of its possible consequences, this complication should not be overlooked.19968924288
pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples.pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ...19968919784
microbiology of the transition from acute to chronic maxillary sinusitis.repeated aspirations of sinus secretions by endoscopy was performed in five patients over a period of 34-50 days and, ultimately, surgical drainage was done in three who presented with acute maxillary sinusitis that did not respond to antimicrobial therapy and became chronic. the aspirates were cultured for aerobic and anaerobic bacteria. most of the bacteria isolated from the first culture were aerobic or facultative bacteria: streptococcus pneumoniae (three isolates), haemophilus influenzae no ...19968918954
microbiology of infected atopic dermatitis.bacterial infections occur frequently in lesions of atopic dermatitis (ad). the objectives of the study were to establish the aerobic and anaerobic microbiology of secondarily infected ad.19968915731
vaginal microflora associated with bacterial vaginosis in japanese and thai pregnant women.the vaginal flora of 118 japanese and 208 thai pregnant women were investigated for the presence of bacterial vaginosis (bv), bv-associated organisms, and bv-associated enzyme. a similar prevalence of bv was found among the japanese (13.6%) and thai women (15.9%). the microbial flora of women with bv were complex; the mean number of isolates recovered in the bv group was approximately 2 times more than that in a group of healthy women. prevotella species, porphyromonas species, peptostreptococcu ...19968909838
a study of the anaerobic bacterial flora of the female genital tract in health and disease.semi-quantitative and qualitative bacterial assessment of the vaginal and cervical flora of a total of 202 women was carried out over a period of six months to determine the bacterial flora in three groups of women and changes caused by prior use of antibiotics. the number was made up of 32 healthy volunteers, 80 women with gynaecological problems and 90 women with gynaecological infections who had had antibiotic treatment prior to this study. standard methods were used for the investigations. f ...19958907604
molecular analysis of microflora associated with dentoalveolar abscesses.the microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16s rrna genes were randomly amplified by means of conserved eubacterial primers and cloned. restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16s rrna from the cultured bacteria was used to detect putative unculturable bacteria. clones representative of five predominant groups of uncultured organisms were sequenced. two were identified as porp ...19968904410
in vitro activity of quinupristin/dalfopristin (rp 59500) against a large collection of infrequently isolated or tested species.quinupristin/dalfopristin (rp 59500, synercid) is a parenteral streptogramin combination antimicrobial that possesses a synergistic and often bactericidal action against many grampositive species. in this study, a collection of 1270 uncommonly isolated or tested strains were evaluated for susceptibility to quinupristin/dalfopristin using agar dilution minimum inhibitory concentration (mic) methods described in the national committe for clinical laboratory standards. the greatest antimicrobial ac ...19968902412
protein pab, an albumin-binding bacterial surface protein promoting growth and virulence.the anaerobic bacterium peptostreptococcus magnus is a human commensal and pathogen. previous work has shown that strains of p. magnus isolated from patients with gynecological disease (vaginosis) frequently express an immunoglobulin (ig) light chain-binding protein called protein l. here we report that strains isolated from localized suppurative infections bind human serum albumin (hsa), whereas commensal isolates bind neither ig nor hsa. the hsa-binding protein pab was extracted from the bacte ...19968900134
in vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.the antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the national committee for clinical laboratory standards-approved wadsworth agar dilution technique. the species tested included bacteroides fragilis (n = 91), other members of the b. fragilis group (n = 130), campylobacter gracilis (n = 15), other bacteroides spp. (n = 16), prevotella spp. (n = 49), porphyromonas spp. (n = 15), fusobacterium spp. (n = 62), bilophila wadsworthia (n = 24), sutterel ...19968878617
deep neck infections. 19968870932
acute phase response in heifers with experimentally induced mastitis.ten pregnant heifers were inoculated in both hind udder quarters with a mixture of actinomyces pyogenes, fusobacterium necrophorum and peptostreptococcus indolicus. development of the experimental mastitis was monitored by sequential clinical and bacteriological examinations, and by blood acute phase protein profiles. sequential changes in plasma fibrinogen, serum haptoglobin, acid-soluble glyco-proteins and alpha 1-proteinase inhibitor activity were analysed and compared with both the clinical ...19968864931
characterization of the binding properties of protein lg, an immunoglobulin-binding hybrid protein.protein lg is a 50-kda hybrid molecule containing four ig-light-chain-binding domains from protein l of peptostreptococcus magnus and two igg-fe-binding repeats from streptococcal protein g. here we analyse the binding of protein lg to ig from several mammalian species. protein lg was shown to bind human igg of all subclasses and other ig classes that carry kappa chains. the binding to human igg was only marginally influenced by changes in temperature (4-37 degrees c) or salt concentration (0-1. ...19968856054
infection of a total knee joint prosthesis by peptostreptococcus micros and propionibacterium acnes in an elderly ra patient: implant salvage with longterm antibiotics and needle aspiration/irrigation.we describe the favourable long-term outcome after late total knee joint prosthesis infection in an elderly ra patient. peptostreptococcus micros and propionibacterium acnes were cultured from the synovial fluid, and the finding of a coexistent abscess at the root of a wisdom tooth suggested a dental origin to the joint infection. long-term antibiotics in conjunction with aspiration/irrigation resulted in salvage of the implant and an excellent functional outcome.19968853177
aerobic and anaerobic microbiology of necrotizing fasciitis in children.specimens obtained from eight children with necrotizing fasciitis (nf) were cultured for aerobic and anaerobic bacteria. a total of 21 isolates were recovered, 13 anaerobic and 8 aerobic or facultatives. the facultative organism streptococcus pyogenes was present alone in two (25%) instances, and mixed aerobic and anaerobic bacteria were isolated in six (75%). the predominant isolates were peptostreptococcus spp. (6 isolates, including 3 peptostreptococcus magnus). s. pyogenes (4), bacteroides f ...19968844744
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