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nucleotide sequence of the gene for peptostreptococcal protein l.a gene bank of peptostreptococcus magnus dna was established using an e. coli host-vector system. western blot analysis identified a clone expressing protein l which bound to the light chain of human immunoglobulins. dna sequence determination and analysis revealed an open reading frame of 992 amino acids, giving a theoretical secreted protein of 106 kd with a pl of 4.67.19947987012
[brain abscess complicating dental caries in children].brain abscess following dental or periapical infection is rare in childhood. this report describes brain abscesses found in two children with dental caries. case 1.--a 12 year-old boy was admitted because he had suffered from acute meningitis for 3 days. clinical examination showed symptoms of meningitis plus palsy of the right third and fourth cranial nerves and of the left facial nerve, and a defect in the left temporal field. funduscopic examination showed papilledema; ct scan and mri showed ...19947987445
prophylactic antibiotic treatment in therapeutic or complicated diagnostic ercp: results of a randomized controlled clinical study.the present study evaluated the effects of prophylactic administration of cefotaxime in patients undergoing therapeutic or complicated diagnostic ercp. one hundred patients were randomized to two groups of 50 patients each. patients in group 1 received an intravenous infusion of 2 g cefotaxime 15 minutes before endoscopy; patients in group 2 did not receive an intravenous antibiotic before ercp (control group). blood samples were drawn for bacteriologic cultures before endoscopy and 5, 15, 30, a ...19947988813
evaluation of the antimicrobial effectiveness of citric acid and sodium hypochlorite on the anaerobic flora of the infected root canal.this study was designed to evaluate the antimicrobial effectiveness of 25% citric acid, compared with 2.5% sodium hypochlorite, after time intervals of 5, 15, 30 and 60 min. anaerobic bacterial species were isolated from infected root canals and categorized according to gram stain as gram-positive cocci, gram-negative cocci, gram-positive rods and gram-negative rods. the experimental method used was similar to that proposed by the german society for hygiene and microbiology (dghm) with slight mo ...19947995646
[an evaluation of pathogens in patients with bronchopulmonary infection by transtracheal aspiration: december 1978-march 1993].we performed transtracheal aspiration (tta) in 1165 patients, who were suspected to have bronchopulmonary infection, from december 1978 to march 1993. we isolated pathogens from tta in 806 patients (69.2%). we isolated h. influenzae (62 cases), s. pneumoniae (39 cases) and m. catarrhalis (24 cases) in patients with acute bronchitis, s. pneumoniae (65 cases), alpha-streptococcus sp. (52 cases), h. influenzae (32 cases) and s. aureus (29 cases) in patients with pneumonia or lung abscess and h. inf ...19947996025
distribution of clindamycin in cortical bone during direct local infusion of the canine tibia.the distribution of clindamycin in tibial cortical bone, administered via direct local infusion with an implantable pump, is described. clindamycin concentrations in cortical bone were measured after 3, 7, and 21 days of intraosseous infusion. the tibia were divided into four quadrants relative to the outflow infusion catheter site located in the medial aspect of the mid-diaphysis. a gradient of 5-30 mm from the infusion site was documented in all four quadrants (proximal lateral, proximal media ...19947996320
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...19948004324
the clinical importance of gram-positive anaerobic cocci isolated at st bartholomew's hospital, london, in 1987.the clinical importance of the gram-positive anaerobic cocci (gpac) isolated in 1987 at st bartholomew's hospital, london, is assessed. of about 800 anaerobic isolates, 209 (27%) were gpac, of which 67 (32%) were from abscesses and 22 (11%) were in pure growth. four species comprised 77% of the 168 isolates available for study: peptostreptococcus magnus (55 isolates, 33%), p. micros (23, 14%), p. asaccharolyticus (24, 14%) p. asaccharolyticus (24, 14%) and p. anaerobius (27, 16%). different spec ...19948006943
an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro.there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ...19957593703
role of anaerobic bacteria in chronic otitis media and cholesteatoma.otitis media (om), a common infection in children, can cause significant morbidity. selection of the most appropriate treatment regimen directed against the pathogens responsible for the om can minimize complications. the most frequently isolated bacteria from chronic om are staphylococcus aureus, pseudomonas aeruginosa and anaerobic bacteria. the predominant anaerobes are peptostreptococcus spp., pigmented prevotella and porphyromonas spp., bacteroides spp. and fusobacterium spp. many of the or ...19957782173
microbiologic and clinical study of polytetrafluoroethylene membranes for guided bone regeneration around implants.this study determined the microbiota of the mucosa- and implant-facing parts of expanded polytetrafluoro ethylene augmentation material, and the influence of major periodontopathogens on the healing process associated with guided bone regeneration around dental implants. seventeen patients with nine dehiscence and eight extraction defects were studied. prior to surgery and at membrane removal, microbial morphotypes, total viable counts, and the occurrence of selected microbial species were exami ...19957615319
[infectious osteoarthritis of the pubis in sportmen. apropos of 2 cases].in athletes, osteoarthritis of the symphysis pubis is an uncommon condition that should be promptly differentiated from pubic pain due to microtrauma. the symphysis pubis is infected via the bloodstream, usually by a staphylococcus. pubic pain with fever and severe disability suggests the diagnosis. increased uptake of bone-seeking radionuclides is an early finding. microbiological and histological studies of specimens obtained under radiological guidance confirm the diagnosis. two new cases of ...19938012337
a quantitative comparison of selected bacteria in human carious dentine by microscopic counts.the levels of selected cariogenic and obligately anaerobic bacteria have been compared in carious dentine taken from fissures, smooth surfaces, and root surfaces. the numbers of infected dentinal tubules were determined by immunohistological staining using species-specific antisera against selected bacteria. selective localization was observed; mutans streptococci were the predominant bacteria in dentine from fissures and smooth surface coronal caries, but not from root surface caries. the propo ...19948033185
bacteria invading periapical cementum.the aim of this study was to determine whether microorganisms invade periapical cementum of human teeth from the adjacent periapical lesions. we therefore attempted to isolate microorganisms from periapical cementum through the adoption of standard anaerobic procedures for obligate anaerobes. samples of cementum were taken from 10 amputated tooth roots at the time of apicoectomy. from two of these samples, bacteria were recovered after anaerobic incubation, but no bacteria were recovered after a ...19948035155
identification of five peptostreptococcus species isolated predominantly from the female genital tract by using the rapid id32a system.the rapid id32a kit (biomérieux vitek, inc., hazelwood, mo.) was evaluated for its ability to identify peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (virginia polytechnic institute), the current "gold standard" method. a total of 5 peptostreptococcus american type culture collection strains and 95 clinical isolates comprising peptostreptococcus anaerobius, p. asaccharolyticus, p. magnus, p. micros, and p. prevotii isolates were included for ...19948051260
case report: anaerobic meningitis caused by peptostreptococcus magnus after head and neck surgery.although anaerobic bacterial meningitis is uncommon, patients subjected to resection of head and neck malignancy appear at special risk. in this article, the authors report on a 72-year-old man in whom meningitis developed after extensive resection of the right sinuses for squamous cell carcinoma; initial treatment consisted of intravenous vancomycin and ceftazidime. intravenous penicillin g was added after the fortuitous early finding of intracellular cocci in wright-giemsa stained cerebral spi ...19948074138
changes in vaginal flora during pregnancy and association with preterm birth.a prospective study of the vaginal flora changes between midtrimester and labor was undertaken in 560 women; 337 had intact membranes and were in labor when swabs were taken. of these, 45 delivered preterm. gardnerella vaginalis, ureaplasma urealyticum (both associated with preterm birth when present in midtrimester), and mycoplasma hominis commonly persisted between midtrimester and labor, particularly in the preterm cohort (24 [65%] of 37 midtrimester isolates), and these organisms were seldom ...19948077737
iron-sulfur cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus: correlation of the cluster type with enzymatic activity.investigations were performed with regard to the function of the iron-sulfur cluster of l-serine dehydratase from peptostreptococcus asaccharolyticus, an enzyme which is novel in the class of deaminating hydro-lyases in that it lacks pyridoxal-5'-phosphate. anaerobically purified l-serine dehydratase from p. asaccharolyticus revealed epr spectra characteristic of a [3fe-4s]+ cluster constituting 1% of the total enzyme concentration. upon incubation of the enzyme under air the intensity of the [3 ...19948082806
an in-vitro study of the comparative viability on different swab types of simulated specimens of bacteria commonly present in oro-facial infections.an in-vitro study was undertaken to investigate the survival on bacteriological swabs of three potentially pathogenic organisms found in oro-facial infections, by using simulated clinical specimens incubated in the presence or absence of amies transport medium. standard inocula of pure cultures of streptococcus milleri, prevotella melaninogenica and peptostreptococcus anaerobius were soaked on to swabs and plated out on to solid media at various intervals up to 3 days, enabling an estimate of th ...19938099810
cytotoxicity versus antibacterial activity of some antiseptics in vitro.through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. a follow-up study was conducted to examine the germicidal effect of 0.05% naocl, 10 mg/ml metronidazole and supernatant of ca (oh)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. in vitro testi ...19938100851
management of maxillofacial infections: a review of 50 cases.maxillofacial infections often place the oral and maxillofacial surgeon in situations where timely decisions have to be made. these decisions can be life-saving. this study reviews 50 infections treated over a 3-year period. the results reveal rapid resolution of the infections by adhering to fundamental principles in their management: recognition of airway compromise, surgical intervention, and the administration of the appropriate antibiotic. a protocol for the management of maxillofacial infe ...19938101564
bacterial and fungal contamination of human fetal liver collected transvaginally for hematopoietic stem cell transplantation.transplantation of fetal hematopoietic stem cells is a new therapy for fetuses diagnosed in utero with inherited disorders. however, prior to transplantation of fetal stem cells, the cells must be free of microbial contamination. in order to investigate the contamination of human fetal liver tissue, we determined the rate and types of bacterial and fungal contamination of human fetal liver tissue collected transvaginally for use in stem cell transplantation by using the us pharmacopoiea assay. w ...19938101709
an unclassified eubacterium taxon in acute dento-alveolar abscess.the microflora of pus samples aspirated from 50 acute dento-alveolar abscesses was examined. a total of 143 bacterial strains was isolated, consisting predominantly of prevotella spp., alpha-haemolytic streptococcus spp., peptostreptococcus spp. and eubacterium spp. an unclassified asaccharolytic eubacterium taxon was encountered in 17 (34%) of the abscesses. this taxon was found to have a positive association with fusobacterium spp. and a negative association with alpha-haemolytic streptococcus ...19948107059
electrogenic glutamine uptake by peptostreptococcus anaerobius and generation of a transmembrane potential.peptostreptococcus anaerobius converted glutamine stoichiometrically to ammonia and pyroglutamic acid, and the eadie-hofstee plot of glutamine transport was biphasic. high-affinity, sodium-dependent glutamine transport (affinity constant [kt] of 1.5 microm) could be driven by the chemical gradient of sodium, and more than 20 mm sodium was required for half-maximal velocity. high-affinity glutamine transport was not stimulated or inhibited by a membrane potential (delta psi). low-affinity glutami ...19948113169
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...19948123556
bacterial colonization and degradation of demineralized dentin matrix in situ.lesion formation in dentin involves demineralization followed by degradation of the exposed organic matrix. proteinases from microorganisms present in the dentin are believed to play an important role in the breakdown of the dentinal collagen. in this study, the microflora colonizing decalcified dentin matrix, placed in situ, was identified. the gelatinolytic activity of the isolated strains was assessed and related to the degradation of the dentin matrix. the predominant species found were stre ...19948124693
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...19948126176
determination of 16s ribosomal rna sequences of all members of the genus peptostreptococcus and their phylogenetic position.a comparative analysis of 16s ribosomal rna sequences of all peptostreptococcus species revealed that most members of the genus peptostreptococcus should be divided into many different genera. the relationship between clostridia and peptostreptococci was analysed to find the phylogenetic position of peptostreptococci.19948132148
in vitro evaluation of the effectiveness of calcium hydroxide and paramonochlorophenol on anaerobic bacteria from the root canal.the effectiveness of calcium hydroxide and paramonochlorophenol (pmcp) was tested on anaerobic bacteria isolated from infected root canals, at time intervals 5, 15, 30 and 60 min. the experimental method used was similar to that proposed by the german society of hygiene and microbiology (dghm), with slight modifications. testing revealed that calcium hydroxide was significantly more effective than pmcp against the total number of anaerobes. additionally, it was proved to be quickly and highly ef ...19938143576
[what kinds of species are in the conjunctiva?].with suitable culture methods less than 5% of conjunctival fluid specimens remain negative. from approximately 95% of the swab cultures at least 65 bacterial species - although in low numbers - were recovered. among the predominant organisms were gram-positive species like several coagulase-negative staphylococcus spp., propionibacterium spp., corynebacterium spp. and members of the genera peptostreptococcus, streptococcus and actinomyces. among the frequently isolated gram-negative species were ...19938144184
[a case of niddm with non-clostridial gas-producing infection in the lower limb--the effects of hyperbaric oxygen therapy].a 36-year-old male was admitted because of swelling and pain in the lower left limb. he had been diagnosed as having non-insulin dependent diabetes mellitus 4 years previously and had been treated unsuccessfully with insulin. since gas formation was radiographically demonstrated in the soft tissue of the left foot, we treated him with antibiotics and hyperbaric oxygen under the diagnosis of gas gangrene. despite this therapy, the gangrenous lesions progressed. amputation of the left foot was nec ...19948151155
[antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections].to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ...19948151907
relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults.to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ...19948157769
protein pab, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling.some strains of the anaerobic human commensal and pathogen peptostreptococcus magnus bind human serum albumin (hsa), whereas other strains of this species express protein l, an immunoglobulin light chain-binding surface protein. a novel hsa-binding protein called protein pab was purified in one step from the culture supernatant of an hsa-binding strain of p. magnus by affinity chromatography on hsa-sepharose. the apparent size of the molecular was 47 kda on sds-polyacrylamide gel electrophoresis ...19948163519
delta 22-beta-muricholic acid in monoassociated rats and conventional rats.bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ...19948177022
antibacterial activity of clavulanate and tazobactam on peptostreptococcus spp. 19948182024
influence of the thymus and the normal microflora on the plasma fibronectin concentration in mice.the plasma fibronectin (pfn) concentration (cc) of untreated genetically or artificially athymic mice, or treated with tp-4 (thymus hormone sequence analog synthetic preparation) showed no significant difference from their euthymic or untreated controls. in contrast, the pfn cc in mice with different microbiological state showed significant alterations; the highest level occurred in conventional mice and the lower level in germfree mice was increased by bacterial monocontamination. the alternati ...19938184670
impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis.the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ...19948195981
bacterial endocarditis in patients with chronic liver disease.although patients with cirrhosis have an increased susceptibility for bacterial infections, endocarditis complicating cirrhosis has been reported only infrequently. in this study, our objective was to determine whether, bacterial endocarditis is, in fact, a complicating factor in cirrhosis.19948198106
[antimicrobial activities of cefuroxime against recent clinical isolates].antimicrobial activity of cefuroxime axetil (cxm-ax) was compared with those of other cephem antibiotics against clinically isolated strains obtained mainly from outpatients of our center in a period from january to september of 1990 and 1993. minimum inhibitory concentrations were determined and the following results were obtained. 1. the results suggested that, compared with reports of studies conducted with clinical isolates in early 1980's, mic80 of cxm were equal to or lower against staphyl ...19948201767
in vitro antibacterial activity of fk037, a new parenteral broad-spectrum cephalosporin, against recent clinical isolates in the fields of obstetrics and gynecology.the antibacterial activity of a new parenteral cephalosporin, fk037 was assessed against recent aerobic and anaerobic strains isolated from patients in the fields of obstetrics and gynecology during the period between january 1992 and june 1993. the mics of fk037 for 90% of the clinical isolates tested were 0.10 microgram/ml for escherichia coli and klebsiella pneumoniae, 0.20 microgram/ml for streptococcus agalactiae, 0.39 microgram/ml for gardnerella vaginalis, 0.78 microgram/ml for staphyloco ...19948205934
[anaerobic respiratory infection--evaluation of methods of obtaining specimens].anaerobic cultures were performed on various kinds of specimens obtained from 67 patients with respiratory infections. the infections consisted of 33 cases of pneumonia, 14 cases of lung abscess, 6 cases of pyothorax, 9 cases of chronic lower respiratory tract infection, and 5 cases of infection of pulmonary cyst. specimens included bronchoscopic specimens obtained using a single tube or protected catheter brush (pcb), transtracheal aspirate, percutaneous lung aspirate, aspirate using a suction ...19948207291
trypsin-dependent production of an antibacterial substance by a human peptostreptococcus strain in gnotobiotic rats and in vitro.an antibacterial substance appeared within 1 day in feces of gnotobiotic rats harboring a human intestinal peptostreptococcus strain. it disappeared when the rat bile-pancreatic duct was ligatured or when the rats ingested a trypsin inhibitor. anaerobic cultures of the peptostreptococcus strain in a medium supplemented with trypsin also exhibited an antibacterial activity, which was also inhibited by the trypsin inhibitor. in vitro the antibacterial substance from both feces and culture medium w ...19938215361
[cytotoxicity of anaerobic bacteria isolated from infected root canal].bacteria in the infected root canal play an important role in the progression of the periapical lesion. the purpose of the present study was to investigate the cytotoxic effects of bacteria isolated from an infected root canal on the periapical lesion. four obligately anaerobic bacteria were isolated and their sonic extracts (se) were prepared. the cytotoxic assay of each se for mc3t3-e1 cell was used with respect to the morphological cell changes and [3h]-thymidine uptake. the following results ...19938228523
epidural spinal abscess containing gas: mri demonstration. 19938232872
cleavages of the o- and c-glucosyl bonds of anthrone and 10,10'-bianthrone derivatives by human intestinal bacteria.a strictly anaerobic bacterium, bifidobacterium sp. sen, capable of hydrolyzing the o-glucosyl of sennosides was isolated from human feces. the bacterium stepwisely hydrolyzed sennoside b to sennidin b through sennidin-8-monoglucoside in pyf medium but not in gam broth. addition of d-glucose to pyf medium resulted in loss of the hydrolyzing activity in culture but addition of d-fructose did not affect the activity. coculture of this bacterium with peptostreptococcus intermedius led to rapid accu ...19938234419
bacterial l-serine dehydratases: a new family of enzymes containing iron-sulfur clusters.two families of enzymes are described which catalyse identical chemical reactions but differ in their prosthetic groups and hence in their mechanism of action. one family, the pyridoxal-5'-phosphate (plp)-dependent l-threonine dehydratases, also use l-serine as substrate. the other, hitherto unrecognized family is the iron-dependent, highly specific bacterial l-serine dehydratases. it has been shown that l-serine dehydratase from the anaerobic bacterium peptostreptococcus asaccharolyticus contai ...19938236444
description of two morphotypes of peptostreptococcus micros.peptostreptococcus micros is often isolated from abscesses in several parts of the human body. the oral cavity is considered the natural habitat for the species, which has been implicated as a periodontal pathogen. in plaque samples from periodontitis patients we observed the presence of a rough morphotype of p. micros in addition to the previously recognized smooth morphotype. the rough morphotype has not been described previously. both morphotypes are frequently isolated simultaneously from th ...19938240959
[study of obligate anaerobic bacterial sensitivity to tinidazole and metronidazole (determination of minimal inhibiting concentration--mic)].comparative susceptibility testing of 428 strains of obligate anaerobic bacteria belonging to genera propionibacterium, arachnia, actinomyces, bacteroides, prevotella, porphyromonas, anaerorhabdus, fibrobacter, fusobacterium, peptostreptococcus and clostridium to metronidazole and tinidazole was performed. the study of the susceptibility of anaerobic bacteria was carried out by the method of serial dilution in brucella agar according to finegold and sutter (1972). strains of b. fragilis species, ...19938249400
antimicrobial activity of dv-7751a, a new fluoroquinolone.we compared the in vitro antibacterial activity of dv-7751a against gram-positive and -negative bacteria with those of quinolones currently available. mics for 90% of the strains tested (mic90s) against clinical isolates of methicillin-susceptible and -resistant staphylococcus aureus and staphylococcus epidermidis were 0.20, 0.39, 0.20, and 0.78 micrograms/ml, respectively. moreover, mic50s for dv-7751a against ofloxacin-resistant methicillin-resistant s. aureus were 4-, 8-, 16-, 32-, and 64-fol ...19938257132
[the role anaerobes in dermatitis digitalis et interdigitalis in cattle].altogether 52 tissue samples and swabs from the corium of the claw of diseased cattle were investigated. microbiological examinations revealed the presence of 11 strictly anaerobic bacterial strains that belonged to the species peptococcus asacharolyticus, pc. sacharolyticus, peptostreptococcus anaerobius, bacteroides asacharolyticus and b. melaninogenicus. the remaining isolates were classified as bacteroides, fusobacterium, streptococcus and clostridium. in the above-mentioned strains observat ...19938259638
role of suspected periodontopathogens in microbiological monitoring of periodontitis.periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. although a specificity to some degree is found for actinobacillus actinomycetemcomitans in localized juvenile periodontitis (ljp), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. what we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshol ...19938260004
microbiology of the pericoronal pouch in mandibular third molar pericoronitis.the microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. the pericoronal pouch was sampled with paper points in a) 8 patients without mandibular third molar pericoronitis and b) 6 patients with mandibular third molar pericoronitis. under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), mot ...19938265205
anaerobic infection in chronic maxillary sinusitis.1) ten per cent of cases of chronic maxillary sinusitis in a series of 50 cases treated in the ent department of dayanand medical college, ludhiana, were caused by anaerobic infection. 2) the causative anaerobic micro-organisms encountered were peptostreptococcus and clostridium species. 3) treatment should, therefore, include metronidazole, especially in those cases refractory to conventional antibiotic therapy.19938270303
treatment of post-cesarean section endometritis with ampicillin and sulbactam or clindamycin and gentamicin.seventy-seven patients were prospectively enrolled in a randomized clinical trial to compare two antimicrobial regimens for the treatment of post-cesarean section endometritis. the two groups were not significantly different with respect to age, race, gravidity, parity, hours in labor, cesarean section indication, preoperative or postoperative hemoglobin/hematocrit, pretreatment white blood cell count or pretreatment temperature. pretreatment urine, blood and endometrial cultures were obtained. ...19938277478
in vitro activity of bay y3118 against anaerobic bacteria.the antimicrobial activity of a new quinolone, bay y3118, was determined against 326 strains of anaerobic bacteria and compared with the activities of ampicillin-sulbactam, cefotetan, clindamycin, imipenem, metronidazole, and sparfloxacin. the national committee for clinical laboratory standards-approved wadsworth agar dilution technique with brucella-laked blood agar was used throughout the study. breakpoints used to determine the percent susceptible were 2 micrograms/ml for bay y3118 and sparf ...19938285647
piperacillin and tazobactam versus clindamycin and gentamicin in the treatment of hospitalized women with pelvic infection. the piperacillin/tazobactam study group.to compare the efficacy and safety of a combination of piperacillin and tazobactam with that of clindamycin and gentamicin in the treatment of hospitalized women with infections of the upper genital tract.19948290195
[multiple hepatic-splenic abscesses. a favorable result with antibiotic treatment].the authors report a case of multiple and spleen pyogenic abscesses caused by peptostreptococcus spp., in a patient who recently underwent laparotomy due to duodenal perforation. he was successfully treated by means of intensive and extended antibiotherapy. we would like to stress the importance of an early and etiological diagnosis performed by percutaneous puncture and bacteriological study, in order to start a selective antibiotherapy. other therapeutical possibilities are discussed.19938292440
recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms.the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ...19938324113
anaerobic bacterial infections of the lung and pleural space.in 193 reviewed cases of pleuropulmonary infections involving anaerobic bacteria, the predominant clinical syndromes were aspiration pneumonia, lung abscess, and empyema. most patients had an indolent infection with tissue necrosis (abscess or bronchopulmonary fistula) at initial presentation. nevertheless, many had a more fulminant disease course resembling that of pneumococcal pneumonia. transtracheal aspiration was the procedure most commonly used to obtain uncontaminated specimens for anaero ...19938324127
bacteriology of anaerobic pleuropulmonary infections: preliminary report.a retrospective bacteriologic study of anaerobic pleuropulmonary infections diagnosed at the wadsworth veterans affairs medical center between 1976 and 1991 was performed. there were 116 specimens from 110 patients. available strains were reexamined using the latest tests and taxonomic schemes. pleural fluid was believed to provide the most reliable specimen; cultures yielded an average of 3.0 anaerobes and 0.6 nonanaerobes per specimen. the most commonly encountered anaerobes were pigmented pre ...19938324128
the normal vaginal flora, h2o2-producing lactobacilli, and bacterial vaginosis in pregnant women.in this study of the vaginal flora of 171 pregnant women in labor at term, the flora was categorized as normal (lactobacillus predominant), intermediate, or representative of bacterial vaginosis (bv) on the basis of a vaginal smear. bv was diagnosed in 39 women (23%); the vaginal flora was classified as normal in 50% of cases and as intermediate in 27%. h2o2-producing lactobacilli were recovered from 5% of women with bv, 37% of those with an intermediate flora, and 61% of those with a normal flo ...19938324131
bacteriologic findings in peritonsillar abscesses in young adults.aspirated pus samples from 124 patients with peritonsillar abscess were cultured quantitatively for aerobes and anaerobes. a total of 98% of the samples yielded bacteria. of the 550 isolates obtained (mean, 4.4 per patient), 143 were aerobes (representing 16 species or groups) and 407 were anaerobes (representing 40 species or groups). aerobes were isolated from 86% of patients-alone in 20 cases and together with anaerobes in 87. the most common aerobic isolates were streptococcus pyogenes (isol ...19938324134
peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report.peritonsillar abscess is a potentially life-threatening complication of acute tonsillitis. on occasion, peritonsillar abscess can extend to neck spaces and/or to the mediastinum. we describe a case of a patient with a peritonsillar abscess that extended to the neck, producing bilateral retropharyngeal abscesses and myonecrosis of the strap muscles. culture of a specimen of the necrotic muscle yielded prevotella intermedia, prevotella buccae, lactobacillus catenaforme, another lactobacillus speci ...19938324135
oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment.microbial populations colonizing the teeth are a major source of pathogens responsible for oral and dental infections, including periodontal diseases, gingivitis, pericoronitis, endodontitis, peri-implantitis, and postextraction infections. each entity has distinct clinical and microbial features. bacterial species associated with oral infections include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, campylobacter rectus, eubacterium ...19938324136
[a case of empty sella syndrome and pan-hypopituitarism associated with acute suppurative paranasal sinusitis]. 19938340667
l-serine and l-threonine dehydratase from clostridium propionicum. two enzymes with different prosthetic groups.l-serine dehydratase from the gram-positive bacterium peptostreptococcus asaccharolyticus is novel in the group of enzymes deaminating 2-hydroxyamino acids in that it is an iron-sulfur protein and lacks pyridoxal phosphate [grabowski, r. and buckel, w. (1991) eur. j. biochem. 199, 89-94]. it was proposed that this type of l-serine dehydratase is widespread among bacteria but has escaped intensive characterization due to its oxygen lability. here, we present evidence that another gram-positive ba ...19938344301
the microbiology of bacterial vaginosis.a group of microorganisms are present concurrently in high concentrations in the vaginas of women with bacterial vaginosis. the major members of the group are gardnerella vaginalis, anaerobic gram-negative rods belonging to the genera prevotella, porphyromonas and bacteroides, peptostreptococcus species, mycoplasma hominis, ureaplasma urealyticum, and often mobiluncus species. facultative species of lactobacillus are present in lower concentrations and are less prevalent in bacterial vaginosis t ...19938357043
lung abscess: analysis of 66 cases from 1979 to 1991.sixty-six patients with bacterial lung abscess were treated between 1979 and 1991 in our hospital. among these patients, death occurred in one of the 42 cases of community-acquired infection (mortality rate: 2.4%) and in 16 of the 24 cases of nosocomial infection (mortality rate: 66.7%). of all 66 cases, 55 were culture-positive, and the etiologic agents isolated from 24 of the culture-positive cases were found to be anaerobic bacteria. the most common aerobes isolated from the foci were identif ...19938358116
purification of antibodies using protein l-binding framework structures in the light chain variable domain.protein l from the bacterial species peptostreptococcus magnus binds specifically to the variable domain of ig light chains, without interfering with the antigen-binding site. in this work a genetically engineered fragment of protein l, including four of the repeated ig-binding repeat units, was employed for the purification of ig from various sources. thus, igg, igm, and iga were purified from human and mouse serum in a single step using protein l-sepharose affinity chromatography. moreover, hu ...19938360508
the influence of race and gender on periodontal microflora.previous studies have demonstrated that demographic characteristics of subject populations influence both the incidence of periodontal diseases and various aspects of host responses to periodontal bacteria. in this study we analyzed the components of the subgingival microflora from individuals with adult periodontitis, early onset periodontitis, gingivitis, and periodontal health as a function of gender and race (black and white). clinical categories were analyzed individually so that there were ...19938387107
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 43-1993. a 71-year-old woman with confusion, hemianopia, and an occipital mass. 19938413415
role of anaerobic bacteria in liver abscesses in children.aspirates from pyogenic liver abscesses obtained from 14 children were cultured for aerobic and anaerobic bacteria. of 29 organisms that were recovered, 17 were anaerobic and 12 were aerobic or facultative. the predominant organisms were peptostreptococcus spp. (5 isolates). bacteroides fragilis group (4), fusobacterium spp. (3) and staphylococcus aureus (4). aerobic or facultative bacteria only were recovered in five cases, anaerobic bacteria only in four and mixed aerobic and anaerobic bacteri ...19938414802
sub-gingival microflora in macaca mulatta species of rhesus monkey.the macaca mulatta species of rhesus monkey is one of several non-human primate (nhp) models for periodontal disease. this report presents the bacteriology of the gingival sulci in m. mulatta monkeys. three sub-gingival sites (maxillary right central incisor, the disto-buccal of the mandibular left second molar and mesio-buccal of the mandibular right second molar) of 9 monkeys were evaluated clinically before scaling and 7 days after scaling. plaque samples were obtained from sub-gingival sites ...19938426283
phylogeny of the ammonia-producing ruminal bacteria peptostreptococcus anaerobius, clostridium sticklandii, and clostridium aminophilum sp. nov.in previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. three isolates, strains c, ft (t = type strain), and sr, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. on the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. in this study, the 16s rrna sequences of these isolat ...19938427801
contemporary management of deep infections of the neck.odontogenic infections rarely lead to involvement of the lateral and retropharyngeal spaces. when this does occur, the microbiology of the infection is similar to the typical odontogenic infection, ie, streptococcus and oral anaerobes including peptostreptococcus, bacteroides, and fusobacterium. there is an increased incidence of fusobacterium seen in the more severe infections, as well as a higher incidence of streptococcus milleri. many patients who have deep cervical infections also have some ...19938445462
metabolism of safflor yellow b by human intestinal bacteria. 19938450320
a review of commonly prescribed oral antibiotics in general dentistry.the microflora associated with odontogenic infections are typically mixed and of indigenous origin. streptococcus, peptostreptococcus, peptococcus, fusobacterium, bacteroides, and actinomyces species are the principle microflora isolated from these infections. penicillin v (phenoxymethyl penicillin) remains the antimicrobial of choice for the initial empirical treatment of odontogenic infections. this agent is safe, highly effective and inexpensive. amoxicillin has little indication for the rout ...19938453530
in vitro and in vivo antibacterial activities of t-3761, a new quinolone derivative.t-3761, a new quinolone derivative, showed broad and potent antibacterial activity. its mics for 90% of the strains tested were 0.20 to 100 micrograms/ml against gram-positive bacteria, including members of the genera staphylococcus, streptococcus, and enterococcus; 0.025 to 3.13 micrograms/ml against gram-negative bacteria, including members of the family enterobacteriaceae and the genus haemophilus; 0.05 to 50 micrograms/ml against glucose nonfermenters, including members of the genera pseudom ...19938460909
proton nuclear magnetic resonance sequential assignments and secondary structure of an immunoglobulin light chain-binding domain of protein l.the 1h nmr assignments have been made for the immunoglobulin (ig) light chain-binding b1 domain of protein l from peptostreptococcus magnus. the secondary structure elements and the global folding pattern were determined from nuclear overhauser effects, backbone coupling constants, and slowly exchanging amide protons. the b1 domain was found to be folded into a globular unit of 61 amino acid residues, preceded by a 15 amino acid long disordered n-terminus. the folded portion of the molecule cont ...19938461301
microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis.a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ...19938463936
[actinomycosis infection of the cerebellum].the case is presented of a 37-year-old male with a cerebellar abscess due to actinomyces israelii. the patient was admitted after dental surgery because of fever and symptoms indicative of raised, intracranial pressure. no abnormalities were detected in peripheral blood and cerebrospinal fluid. ct scan revealed a space-occupying lesion in the left cerebellar hemisphere with perifocal oedema and occlusive hydrocephalus. after emergency external ventriculostomy complete removal of the mass was ach ...19938333207
on the interaction between protein l and immunoglobulins of various mammalian species.protein l, a cell wall molecule of certain strains of the anaerobic bacterial species peptostreptococcus magnus, shows high affinity for human immunoglobulin (ig) light chains. in the present study protein l was tested against a panel of human myeloma proteins of the igg, igm, iga and ige classes, and strong binding was seen with antibodies carrying kappa light chains. a high degree of specificity for ig was demonstrated in binding experiments with human plasma proteins. apart from human ig, str ...19938469922
[recent bacteriologic data: from physiopathology to treatment].bacterial vaginosis is characterized by a change in the vaginal ecosystem in which lactobacillus spp, the dominant members of the normal flora, are replaced by an association of various bacterial species including gardnerella vaginalis, anaerobes (bacteroides spp, prevotella spp, porphyromonas spp, peptostreptococcus spp and mobiluncus spp) and mycoplasma hominis. the reasons for this imbalance are unknown, although the loss of lactobacilli that produce hydrogen peroxide (which is toxic for g. v ...19938484089
spinal epidural abscess: unusual ct and microbiologic findings.the incidence of spinal epidural abscess is increasing. its expeditious diagnosis is essential if treatment is to be effective. the authors present a case in which both the computed tomography appearance and the microbiologic observations were unusual.19938504337
intrapleural streptokinase in experimental empyema.intrapleural streptokinase has been used in multiloculated empyemas to enhance pleural space drainage, presumably by causing fibrinolysis of the interlocular septae. we evaluated the efficacy and safety of daily administration of 10,000 u intrapleural streptokinase or equal volumes of saline to enhance resolution of experimental empyema in the rabbit pleural space. seventy-two hours after intrapleural turpentine, 10(8) colony-forming units each of escherichia coli, peptostreptococcus anaerobius, ...19938466134
antibacterial activity of oral antibiotics against anaerobic bacteria.the spectrum and the antibacterial susceptibility of anaerobic bacteria to oral antibiotics isolated from clinical specimens was assessed in two different centres, the first receiving specimens from university departments and the second from general practitioners and small hospitals. susceptibility was studied with a microtiter ready-to-use panel system, using the manufacturer's modified wilkins-chalgren's broth as the test medium for the following antibiotics: ampicillin, ampicillin+sulbactam, ...19938508686
peptostreptococcus micros has a uniquely high capacity to form hydrogen sulfide from glutathione.there are high amounts of hydrogen sulfide in deep periodontal pockets. this volatile sulfur compound may be formed from l-cysteine, but only low levels of this amino acid can be expected to be present in periodontal pockets. glutathione, l-gamma-glutamyl-l-cysteinylglycine, is in high concentration in most tissue cells, and this tripeptide may be more readily available as a source of hydrogen sulfide formation in the pockets. the ability of 37 different species of oral bacteria to utilize gluta ...19938510983
[bacteriological and clinical studies in 23 cases of thoracic empyema--the role of oral streptococci and anaerobes].the bacteriology of empyema fluid and the clinical background of 23 cases from july 1987 through july 1992 were studied. nineteen cases were male and 4 female, with a mean age of 59.6 years (range; 33 to 84 y.o.). there were 15 cases of community-acquired infection and 8 of nosocomial infection. acute pneumonia and/or lung abscess developed into empyema in 19 cases. sixteen cases had associated predisposing conditions, such as diabetes, chronic bronchitis, disorders with dysphagia, and excess al ...19938515622
infected epidermoid cyst of the sphenoid bone.an infected epidermoid cyst presented on ct as a primarily dense, sclerotic expansile lesion in the greater wing of the sphenoid bone. presumably, infection was responsible for the atypical appearance.19938517372
in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria.the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ...19938517721
[studies on the locality and the function of spirosome in bacterial cells: relationship between the production of spirosome and anaerobic glycolysis].the locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and electron microscopy in peptostreptococcus productus, clostridium sp. hd-17, lactobacillus fermentum, eubacterium aerofaciens and escherichia cali b. when the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of ...19938315808
bioactivation of cysteine conjugates of 1-nitropyrene oxides by cysteine conjugate beta-lyase purified from peptostreptococcus magnus.to determine the role of cysteine conjugate beta-lyase (beta-lyase) in the metabolism of mutagenic nitropolycyclic aromatic hydrocarbons, we determined the effect of beta-lyase on the mutagenicities and dna binding of cysteine conjugates of 4,5-epoxy-4,5-dihydro-1-nitropyrene (1-np 4,5-oxide) and 9,10-epoxy-9,10-dihydro-1-nitropyrene (1-np 9,10-oxide), which are detoxified metabolites of the mutagenic compound 1-nitropyrene. we purified beta-lyase from peptostreptococcus magnus gai0663, since p. ...19958526486
structures of bacterial immunoglobulin-binding domains and their complexes with immunoglobulins.three-dimensional structures are now available for several immunoglobulin binding domains from bacterial proteins a, g, and l. x-ray diffraction and nmr experiments on complexes of these domains with portions of immunoglobulins have revealed common structural themes used in these interactions. these data expand our understanding of structure/function relationships in these molecular recognition processes and provide the basis for rational design of artificial immunoglobulin-binding molecules.19958528763
[clinical study of anaerobic respiratory infection].this clinical study involved 35 cases, anaerobic bacteria were detected by tta (transtracheal aspiration) or percutaneous lung aspiration, or pleural puncture. these cases were treated over the last 8 years in our department. there were 9 empyema, 9 pneumonitis, 5 lung abscess, 1 necrotizing pneumonia and 11 chronic lower airway infection. in 13 cases (37%), anaerobic bacteria alone were detected, whereas both anaerobic and aerobic bacteria were observed in the other 22 cases (63%). of all bacte ...19938315301
competition for peptides and amino acids among periodontal bacteria.we recently studied the utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine), l-cysteinylglycine and l-cysteine by anaerobic bacteria. the rate of hydrogen sulfide formation from these compounds was determined and it was concluded that peptostreptococcus micros and fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. in the present study it is shown that methyl mercaptan formation from l-methionine and l-methionyl-containing peptides can also be used to ...19958544102
aerobic and anaerobic microbiology of superficial suppurative thrombophlebitis.to study the aerobic and anaerobic microbiologic characteristics of superficial suppurative thrombophlebitis.19968546586
treatment of peptostreptococcus micros endocarditis with teicoplanin. 19958562764
a case of anaerobic pericarditis. 19958562770
microbiology of mediastinitis.to study the microbiologic and clinical characteristics of patients with mediastinitis.19968572845
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