purification, new assay, and properties of coenzyme a transferase from peptostreptococcus elsdenii.coenzyme a (coa) transferase from peptostreptococcus elsdenii has been purified and crystallized, and some of its properties have been established. the work was facilitated by a newly developed coupled and continuous spectrophotometric assay in which the disappearance of added acrylate could be followed at 245 nm. the rate-limiting conversion of acetyl- and beta-hydroxypropionyl coa to acrylyl coa by coa transferase was followed by the non-rate-limiting conversion to beta-hydroxypropionyl coa by ...1975369
survival of anaerobic bacteria during transportation. 1. experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen.the effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4 per cent. three b. fragilis strains and one strain of fusobacterium mortiferum and of peptostreptococcus anaerobius were investigated. bacterial recovery was determined one hour and 24 hours after evacuation of atmospheric air by pure carbon dioxide and p ...19763089
bacterial interference by oropharynegeal and clinical isolates of anaerobic bacteria.anaerobic isolates were tested for bacterial inhibitory activity. of 144 isolates, 102 were from oropharynegeal washings, and 42 were from clinical specimens. thirteen facultative bacterial species (seven members of the enterobacteriaceae and six species of gram-positive cocci) were used as indicators of inhibition. eleven anaerobic species were isolated from oral secretions. all isolates of bacteroides melaninogenicus, the most commonly recovered species, consistently inhibited several species ...197610336
studies on the binding of fmn by apoflavodoxin from peptostreptococcus elsdenii, ph and nacl concentration dependence.1. the ph and ionic strength dependence of the interaction of fmn with apoflavodoxin has been studied by fluorometry in the ph region 2-5, at 22 degrees c. 2. the rate constant of dissociation and the dissociation constant were experimentally determined; the rate constants of association were claculated at a given ph value. these constants depend on the ionic strength. the plots of these constants against the square root of the ionic strength are straight. 3. our data have been interpreted in te ...197610978
the syndrome of inappropriate secretion of antidiuretic hormone associated with anaerobic thoracic empyema.the syndrome of inappropriate secretion of antidiuretic hormone has been associated with many pulmonary diseases, including tuberculosis and bacterial and viral pneumonia: however, it has not been reported with anaerobic infections or empyema in the absence of pneumonia. we report a patient with empyema due to bacteroides melaninogenicus, bacteroides oralis, and peptostreptococcus who developed the syndrome. eight hours before the start of therapy, his serum sodium concentration was 127 meq per ...197715491
in vitro inhibition of growth of neisseria gonorrhoeae by genital microorganisms.the ability of microorganisms present in titers of over 10(5)/ml in the vaginal or cervical secretions to inhibit growth of n. gonorrhoeae in vitro was tested. study of a strain of n. gonorrhoeae against 77 microorganisms demonstrated that most strans of s. epidermidis, s. aureus and the one "gaffkya anaerobia" interfered with the growth of the n. gonorrhoeae. a minority of strains of s. viridans, neisseria, candida and bifidobacterium demonstrated interference. no strains of enterococcus, dipth ...197717163
effect of pressure upon the fluorescence of various flavodoxins.the effects of hydrostatic pressure in the range of 10(-3) to 11 kbar on the fluorescence of flavodoxins from peptostreptococcus elsdenii, desulfovibrio vulgaris, azotobacter vinelandii, and clostridium mp were investigated. the first three flavoproteins showed under high pressure enhancements of flavin fluorescence of over 50 times resulting from the release of flavin mononucleotide from the protein complex. the clostridial flavodoxin showed a very much smaller fluorescence change. at ph 7.5 th ...197720943
current aspects of gas gangrene, apropos of 47 cases collected over a 3-year period (1974-1976).on the basis of 47 cases of gas gangrene collected over the three year period between 1974 and 1976, the authors review the circumstances surrounding its development, the clinical features and the prognosis of the disorder which remains grave despite a well-defined therapeutic protocol combining surgery, antibiotics and hyperbaric oxygen. there would appear to be a real resurgence of the disease at the present time. post-traumatic and surgical aetiologies predominate, giving rise to two types of ...197723023
quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ...197824059
hydrogen peroxide and superoxide radical formation in anaerobic broth media exposed to atmospheric oxygen.fourteen different broth media were autoclaved under anaerobic conditions and then exposed to atmospheric oxygen. the hydrogen peroxide and superoxide radical formation as well as the bactericidal effect of the media were studied. the rate of killing of peptostreptococcus anaerobius vpi 4330-1 was high in media that rapidly autoxidized and accumulated hydrogen peroxide. in actinomyces broth (bbl), 50% of the cells were killed within 2 min, and in brewer thioglycolate medium (difco), 50% were kil ...197829560
acute suppurative parotitis caused by anaerobic bacteria: report of two cases.staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. it may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. we describe two cases of acute suppurative p ...197832513
improved procedure for transport of dental plaque samples and other clinical specimens containing anaerobic improved transport system for samples containing anaerobic bacteria was developed. this system increased the recovery rate of anaerobic bacteria up to 28.8% as compared to a commonly used method.197939087
differentiation of several isolates of peptococcus magnus by counterimmunoelectrophoresis.uniform sonicated suspensions of three clinical isolates of peptococcus magnus that were morphologically, chromatographically, and biochemically identical were used to produce hyperimmune antisera in rabbits. similar hyperimmune serum was prepared to a single strain of peptostreptococcus anaerobius. when these antisera and antigens were tested by counterimmunoelectrophoresis, the three isolates of peptococcus magnus were immunologically distinct. none of the antisera to peptococcus magnus formed ...197768929
extractable antigen shared by peptostreptococcus anaerobius strains.extracts from several species of gram-positive cocci were prepared by a modification of the rantz-randall autoclave method and tested for reactions with rabbit anti-peptostreptococcus anaerobius (atcc 27337 and vpi 5737) sera in a capillary precipitin test. antigen preparations from two reference strains of p. anaerobius (atcc 27337 and vpi 5737) and six clinical isolates of p. anaerobius reacted with the p. anaerobius antisera. these extracts formed a line of identity by immunodiffusion and dis ...197988461
identification of bacteroides fragilis by indirect immunofluorescence.rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ...197994651
a new hydrolase specific for taurine-conjugates of bile acids.through the investigation of the bile acid-deconjugation activities of human intestinal anaerobes, a new enzyme was discovered in peptostreptococcus intermedius which hydrolyzed specifically the taurine-conjugates, but not the glycine-conjugates of bile acids. however, the enzymes in streptococcus faecalis and lactobacillus brevis hydrolyzed chiefly the glycine-conjugates.1978100491
the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy.quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ...1979118996
predominant cultivable microbiota in periodontosis. 1977138729
bacteriological studies of developing supragingival dental plaque. 1977138733
susceptibility of anaerobic bacteria to metronidazole: relative resistance of non-spore-forming gram-positive baccilli.susceptibility of 358 clinical isolates of obligate anaerobes to metronidazole was determined by an agar-dilution technique. only 66% of all isolates were inhibited by 6.25 mug/ml, whereas 30% required larger than or equal to 50 mug/ml. considerable variation in susceptibility was observed among different genera and species of bacteria. fusobacterium was most senstitive, followed by clostridium, bacteroides and peptococcus, peptostreptococcus, veillonella and acidaminococcus, and non-spore-formi ...1975163867
prophylactic antibiotics in cesarean section.using a random selection procedure, 100 patients were evaluated in order to determine the value of prophylactic antibiotics in cesarean section. all patients were evaluated for febrile morbidity, and specific infection sites were recorded. significant differences were found between the treated and control groups and between cesarean sections performed prior to the onset of labor and after the onset of labor.1975164642
antibiotic susceptibility testing of anaerobic bacteria by the standardized disc diffusion method with special reference to bacteroides fragilis.the susceptibility of 90 strains of bacteroides fragilis to 16 antibacterial drugs was determined by the agar plate dilution test and the disc diffusion test. good correlation of results was obtained with the two methods, and regression line analysis could be performed for beta-lactam antibiotics, erythromycin, tetracyclines, lincomycin and clindamycin. thus the standardized disc diffusion method can be used for susceptibility testing, but only a clear distinction between sensitive and resistant ...1975167431
growth curves of anaerobic bacteria in solid media.simple pour plate and spectrophotometric techniques for the evaluation of growth curves of several anaerobic bacteria on solid media are described. three basic patterns of anaerobic growth were observed. the curves obtained were very reproducible when studied on separate occasions. the curves obtained by spectrophotometric measurement were comparable to those obtained by the pour plate method, especially when a large bacterial inoculum was used. limitations in the interpretation of the results a ...1975167660
electron-transferring flavoprotein of peptostreptococcus elsdenii that functions in the reduction of acrylyl-coenzyme peptostreptococcus elsdenii, a three-component flavoprotein electron transfer system catalyzes the oxidation of lactate and the reduction of crotonyl-coenzyme a (coa). spectral evidence showed that d-lactate dehydrogenase, when reduced by d-lactate, was able to reduce butyryl-coa dehydrogenase, but only in the presence of the electron-transferring flavoprotein. reduced nicotinamide adenine dinucleotide could replace reduced d-lactate dehydrogenase. a reconstituted system, containing the three ...1975172488
evaluation of the sodium polyanethol sulfonate disk test for the identification of peptostreptococcus anaerobius.the previously reported sodium polyanethol sulfonate disk test for the identification of peptostreptococcus anaerobius (graves et al., 1974) was evaluated, with modifications. three bands of brucella agar, three inoculum sizes, and two inoculum sources were compared. nine stock cultures of p. anaerobius (eight normal flora isolates and atcc 27337) and 16 fresh clinical isolates were used. all cultures of p. anaerobius showed inhibition zones of 12 to 30 mm in diameter, regardless of test conditi ...1976185236
anaerobic bacteria in biliary disease in elderly patients.gallbladder bile from 52 elderly subjects who had undergone biliary tract surgery was examined for the presence of bacteria. twelve patients had sterile bile, 18 specimens of bile yielded anaerobes as well as aerobes, and 22 yielded aerobic bacteria only. escherichia coli was the most commonly isolated organism (30 strains). bacteroides fragilis was the most frequently encountered anaerobic bacterium and was found in 15 patients. the klebsiella-enterobacter group was the second most commonly iso ...1977192815
a continuous fluorometric assay for flavokinase. properties of flavokinase from peptostreptococcus elsdenii.a continuous fluorometric assay that utilizes apoflavodoxin as a trapping agent for riboflavin 5'-phosphate (fmn) has been developed for flavokinase (atp:riboflavin 5'-phosphotransferase, ec use of this assay is illustrated in a procedure for the partial purification of flavokinase from the strict anaerobe peptostreptococcus elsdenii. the purified enzyme catalyzed the formation of 8.3 nmol fmn - min-1 - mg-1 at 37 degrees c and had apparent km values for riboflavin and atp of 10 and 4 ...1977195619
gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on neisseria meningitidis in blood culture media.the inhibitory effect of sodium polyanethol sulfonate (0.05%) upon growth of neisseria meningitidis was found to be neutralized by adding gelatin (l.1%) to the growth medium. the neutralizing effect was demonstrated in solid medium, as well as in nutrient broth for blood cultures. the findings parallel those of wilkins and west (6) regarding gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on peptostreptococcus anaerobius.1977195978
[fecal flora of man. v. communication: the fluctuation of the fecal flora of the healthy adult (author's transl)].seven fecal specimens from each of healthy persons, aged 25 to 42 years, during the period of 2 months were analyzed to obtain the knowledge on the day-to-day variation in the same person and the person-to-person variation. irrespective of individuals, bacteroidaceae, catenabacteria (eubacteria and strict anaerobic lactobacilli), peptostreptococci (anaerobic gram positive cocci) and bifidobacteria constituted the predominant flora. spirillaceae, megasphaerae and clostridia (except cl. perfringen ...1977196456
the in vitro and in vivo activity of taurolin against anaerobic pathogenic antibacterial agent, taurolin, formed by the combination of 2 molecules of taurine with 3 molecules of formaldehyde has been tested against anaerobes in vitro and in vivo. in vitro, taurolin is highly active against bacteroides species and anaerobic streptococci as measured by the minimum inhibitory concentration. in vivo, small dosages of taurolin given intraperitoneally increase the mortality in mice with bacteroides peritonitis. however, by increasing the dosage of taurolin, survival is pr ...1977201040
rubredoxin. 1978213684
effect of silver nitrate application on the conjunctival flora of the newborn: and the occurrence of clostridial conjunctivitis.newborn conjunctival cultures were obtained from 35 babies prior to silver nitrate application and 48 hours later. on initial culture, 46 facultative bacteria and 27 anaerobes were recovered; 48 facultative and 18 anaerobes were recovered after 48 hours. haemophilus vaginalis, bacteroides species and anaerobic cocci decreased in numbers, whereas s. epidermidis, micrococcus and propionibacterium acnes increased during this time interval. clostridial species were isolated from two cases who develo ...1978216788
occurrence of anaerobic bacteria in diseases of the dog and cat.a survey for anaerobic bacteria was conducted in 314 clinical specimens from dogs and cats. a total of 187 anaerobic isolates in pure and mixed culture were isolated from 111 of the specimens that contained anaerobic bacteria. common isolated included actinomyces (9.1%), clostridium perfringens (19.3%), other clostridium spp (11.2%), peptostreptococcus anaerobius (7.5%), bacteroides melaninogenicus (13.4%), other bacteroides spp (17.6%), and fusobacterium necrophorum (5.3%). anaerobic bacteria w ...1979224739
[surgical infections by anaerobes in gyneco-obstetrics]. 1978232933
[sensitivity of anaerobes in gyneco-obstetric infections]. 1978232934
role of anaerobic bacteria in subdural empyema. report of four cases and review of 327 cases from the english literature.anaerobic bacteria were isolated from the subdural space in all four cases of subdural empyema encountered over a 2 and a half year period. only one aerobe was isolated in these cases. the bacteriology of subdural empyema was further analyzed from a review of 327 cases reported in the english literature. anaerobes accounted for 12 per cent of 234 cases; in addition, 27 per cent of cases were reportedly "sterile." these data support our finding that anaerobic bacteria may play a far more importan ...1975234678
[new species of lactic acid bacteria, described in the ussr, in the light of modern identification schemes]. 1975235331
d-lactate dehydrogenase of peptostreptococcus elsdenii. 1975236453
evaluation of bacteriological transport commercially manufactured bacteriological transport systems, including culturette, trans-cul (with stuart and amies), handiswab, securline (with amies and amies without charcoal) and culture caddy, were evaluated to determine whether these systems were capable of maintaining the viability and constant numbers of mixtures of hardy, fastidious and anaerobic organisms over 72 hours at 25c and 4c. the results of the study indicated that if specimens are maintained in transport systems at 25c t ...1975239597
survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems.the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ...1975240870
dental caries in gnotobiotic rats inoculated with a strain of peptostreptococcus intermedius. 1977264886
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
dental caries in gnotobiotic rats inoculated with a strain of peptostreptococcus intermedius. 1978277560
dental caries in gnotobiotic rats incubated with a strain of peptostreptococcus intermedius. 1978280581
post-tonsillectomy anaerobic septicemia. 1979286176
long-term effects of tetracycline on the subgingival microflora. 1979287678
metastatic peptostreptococcus intermedius endophthalmitis after a dental procedure.a 46-year-old man developed symptoms of a chronic progressive uveitis in his right eye approximately one week after a dental procedure. the patient's intraocular inflammation was not diminished by massive treatment with topical and systemic corticosteroid therapy or intravenously administered adrenocorticotropic hormone. the inflammatory process progressed to an overt endophthalmitis during a period of three weeks and the eye eventually required evisceration. a pure culture of peptostreptococcus ...1978306760
anaerobic and aerobic bacteriology of acute conjunctivitis.aerobic and anaerobic cultures and clinical data were obtained from 131 patients presenting with acute conjunctivitis. similar cultures were obtained from 60 noninflamed individuals. anaerobes were isolated from 66 patients, 51 times in mixed culture with aerobes and 15 times (11.5%) as the only isolates. aerobes only were recovered in 54 patients. the organisms found to be statistically significantly more commonly recovered from eyes with conjunctivitis were staphylococcus aureus, hemophilus in ...1979313179
bactericidal activity of tinidazole. an in vitro comparison of the effects of tinidazole and metronidazole against bacteroides fragilis and other anaerobic bacteria.the new antiprotozoal agent, tinidazole, was found to be bactericidal against all 52 isolates of obligate anaerobic bacteria tested, 42 bacteroides fragilis, 4 clostridia and 6 peptostreptococci. the minimum bactericidal concentrations of tinidazole for b. fragilis ranged from 0.25 to 4 mug/ml, and those of metronidazole from 0.25 to 8 mug/ml, i.e. several times lower than the serum concentrations achievable after oral administration. in most cases the mic was identical with mbc or half of it. o ...1977318974
[growth of non-sporing anaerobes in an oxygen-free blood culture system (author's transl)].the efficacy of the commercially available vacutainer blood culture system to support the growth of non-sporing anaerobes was compared with two laboratory-prepared blood culture media (supplemented thioglycollate medium and brain heart infusion). the media were inoculated with 10, 100 and 1000 organisms of the species tested, and the number of colony-forming units was determined at intervals of 8-10 hrs. analogous experiments were performed with batches of the media to which 10% vol/vol of human ...1977325956
necrotizing fasciitis in postpartum patients. a report of four cases.necrotizing fascitis is a rare but rapidly progressive soft tissue infection not previously reported in an obstetric patient. since cure is dependent on rapid recognition and early surgical intervention, 4 cases are reported herein, the bacteriology discussed, and the literature reviewed.1977337196
bacteriological findings in cultures of clinical material from bartholin's abscess.purulent exudate from 28 cases of bartholin's abscess were examined for aerobic, anaerobic, and microaerophilic bacteria. three cases gave no bacterial growth, five cases grew facultative bacteria only, and a further three grew a mixture of facultative and anaerobic bacteria. one case gave a pure growth of a microaerophilic streptococcus. anaerobic bacteria were the only isolates in 16 cases and bacteroides species were the most common organisms isolated; they were present as 62.5% of the total ...1977338640
eikenella corrodens subacute bacterial endocarditis: mixed infection in amphetamine user. 1977340988
the bacteriology of cavitating pulmonary infections and empysema. studies of transtracheal aspirates and pleural fluid.a prospective study of bacteriology in 14 cases of cavitating pulmonary infections and empyema is reported. bacteriologic results were based on transtracheal aspirates or pleural fluid. appropriate anaerobic bacteriologic methods were employed. anaerobic bacteria were recovered in 11 patients (79%); they were the only pathogens isolated in 6 patients. the predominant species were fusobacterium nucleatum, bacteroides melaninogenicus, bacteroides fragilis and peptostreptococcus. aerobic bacteria w ...1978352421
antimicrobial effect of amniotic fluid.the antimicrobial effect of amniotic fluid (af) obtained during the first (af1) and second (af2) trimesters was compared with the third (af3) against anaerobic bacteria, such as bacteroides fragilis ss. fragilis (6 strains), eubacterium lentum (3 strains), and peptostreptococcus anaerobius (4 strains). escherichia coli (5 strains) served as a positive control. af1 supported the growth of all 4 anaerobes (except b fragilis for 4 hours) for the entire 24-hour period tested. af2 supported the growt ...1978355963
effects of storage in an anaerobic transport system on bacteria in known polymicrobial mixtures and in clinical anaerobic transport system (ats) which provides for catalytic removal of oxygen was evaluated by using in vitro-prepared polymicrobial mixtures of logphase bacteria and clinical specimens. inoculated swabs were stored at room temperature in (i) aerobic, (ii) anaerobic glove box, and (iii) ats environments, and bacteria were quantitated after 2, 24, 48, and 72 h. bacteria in a three-part mixture of bacteroides fragilis, peptostreptococcus anaerobius, and escherichia coli and in a five-part mix ...1978370142
perirectal abscess in childhood. a review of 29 cases.a total of 29 cases of perirectal abscess at milwaukee children's hospital between 1965 and 1975 were retrospectively reviewed. one fourth of these children had a serious underlying chronic disease. staphylococcus aureaus was the organism that grew most frequently from pus obtained from the abscesses, accounting for 52% of the isolates. therapy included incision and drainage alone and incision and drainage plus parenteral and/or oral antibiotic treatment. one patient, a child with leukemia, died ...1979373420
tyrosine degradation in presumptive identification of peptostreptococcus anaerobius.a new tyrosine medium was developed and evaluated for the differentiation of peptostreptococcus anaerobius from other anaerobic, gram-positive cocci. the strains included 159 originating from clinical specimens and 13 reference strains received from other workers in the field. only one strain of each species was included in the study from multiple cultures from the same patient. this medium is simple to prepare and can be used in a small clinical laboratory. one hundred seventy-two strains of an ...1979379031
[bacteriological aspects of trichomonal vaginitis (author's transl)].an analysis was made of the vaginal flora of 25 gynaecological patients with acute trichomomal vaginitis, with the view to elucidating the bacteriological situation. eighty-four isolates, an average of 3.4 per patient, were taken and included a wide range of aerobic and anaerobic bacteria. - obligate anaerobic species, such as bacteriodes and peptostreptococci, with susceptibility to metronidazole were among the predominant pathogens. - the differentiated susceptibility of the most common bacter ...1979386649
the prevalence of anaerobic bacteria in suprapubic bladder aspirates obtained from pregnant women.the incidence of asymptomatic bacteriuria due to aerobic, facultative anaerobic and strictly anaerobic bacteria during pregnancy was determined in 185 patients admitted for complications of pregnancy, using suprapubic bladder aspiration. anaerobic bacterial counts of 103 per ml urine or higher were detected in 6.4 per cent of the specimens. the incidence of anaerobic bacteriuria seems comparable to that of bacteriuria due to aerobic and facultative anaerobic micro organisms. in our group of pati ...1979388942
[technics for isolation, incubation and preliminary identification tests].in this report the aa. emphasize the fact that anaerobes are mostly isolated from mixed cultures in which several bacteria are present. therefore they suggest to grow all the bacteria under the best conditions and consequently to select the anaerobes from the aerobes. furthermore they describe the selecting techniques used at istituto superiore di sanità and clinica medica iii, to attain this aim and enumerate the best methods used for anaerobic incubation.1979399835
amino acid sequence of desulfovibrio vulgaris flavodoxin.the complete amino acid sequence for the 148-amino acid flavodoxin from desulfovibrio vulgaris was determined to be: h3n+-met-pro-lys-ala-leu-ile-val-tyr-gly-ser-thr-thr-gly-asn-thr-glu-tyr-thr-ala-glu-thr-ile-ala-arg-glu-leu-ala-asn-ala-gly-tyr-glu-val-asp-ser-arg-asp-ala-ala-ser-val-glu-ala-gly-gly-leu-phe-glu-gly-phe-asp-leu-val-leu-leu-gly-cys-ser-thr-trp-gly-asp-asp-ser-ile-glu-leu-gln-asp-asp-phe-ile-pro-leu-phe-asp-ser-leu-glu-glu-thr-gly-ala-gln-gly-arg-lys-val-ala-cys-phe-gly-cys-gly-as ...1977402366
metronidazole therapy of anaerobic bacteremia, meningitis, and brain abscess.four patients with bacteroides fragilis bacteremia, one patient with a brain abscess due to bacteroides species, fusobacterium naviforme, and peptostreptococcus species, and an infant with bacteroides species ventriculitis and meningitis were treated with metronidazole. in all cases the anaerobic pathogens were eradicated. five of the six patients recovered. one patient with leukemia in whom b fragilis bacteremia was eradicated by metronidazole treatment subsequently died of pseudomonas aerugino ...1979434970
aerobic and anaerobic bacterial flora of the maternal cervix and newborn gastric fluid and conjunctiva: a prospective study.aerobic and anaerobic cultures were performed on cervical swabs from 35 mothers and their newborn infants' gastric aspirates and conjunctival sacs. four hundred seventy-nine isolates were obtained; 287 were aerobes and 192 were anaerobes. the cervical and gastric cultures overall yielded a similar flora, but this was not always the case when one compared mothers with their own newborn infants. the conjunctival cultures yielded about half of the number of bacteria per specimen that the gastric co ...1979440845
[short-term oral chemoprophylaxis before intestine surgery. quantitative determination of bacteria and fungi in stool specimens (author's transl)].the methods of quantitative analysis of aerobe and anaerobe microbes and fungi stool specimens are described. the results of the studies in health people are compared to the results in patients undergoing surgical treatment of intestinal tract. a group of these patients received neomycin and bacitracin orally as short-time chemoprophylaxis to diminish possible woundinfection and/or sepsis. after oral medication germs as bifidobacterium, bacteroides and clostridium (not cl. perfringens) are reduc ...1979452770
bactericidal effect of cysteine exposed to atmospheric oxygen.peptostreptococcus anaerobius vpi 4330-1 was exposed to atmospheric oxygen in a dilution bland (0.2% gelatin, salts, resazurin) solution. the organisms were rapidly killed when the solution contained cysteine. the organisms were effectively protected by catalase and horseradish peroxidase as well as by the metal ion-chelating agents 8-hydroxyquinoline and 2,2'-bipyridine. superoxide dismutase increased the rate of killing of the organisms, whereas singlet oxygen quenchers and scavengers of hydro ...1979453819
isolation and characterisation of bacteria from abscesses in the subcutis of cats.thirty-six closed abcesses in the subcutis of cats were examined. of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes only. bacteriodes was the genus most commonly isolated (28.6% of all isolates) followed by fusobacterium (19.0%) and pasteurella (multocida) (13.1%). peptostreptococcus anaerobius was ...1979458840
[first steps in the bacterial colonization of the digestive tract of neonates (author's transl)].meconium samples obtained from 14 children aged 18 to 51 h were subjected to a quantitative differential analysis. the meconium of one of the children (47 h old) was sterile; in those of the 11 infants aged less than 47 h the dominant flora was composed of either enterobacteria, mainly escherichia coli sensitive to usual antibiotics, or of streptococci. the genus acinetobacter constituted the dominant flora in one case only. staphylococcus, corynebacterium, clostridium (c. perfringens), bacteroi ...1979475219
description of strains of peptostreptococcus anaerobius isolated from subcutaneous abscesses in cats.strains of peptostreptococcus, streptococcus and of a gram-positive coccus, which was initially isolated as an anaerobe but grew subsequently as a facultative organism, were isolated from subcutaneous abscesses in cats. the cat strains of peptostreptococcus gave metabolic fermentation products in combinations described for p. anaerobius. the streptococcus strains conformed to the group s. intermedius. the facultative organism described had the metabolic products of p. anaerobius but the distinct ...1979479840
[bacteriological diagnosis of anaerobic infections (author's transl)].a review on anaerobic bacteria with special reference to their role in human disease and an outline for clinical laboratory practice (collection of the specimens, culture media, isolation, identification, antibiotic sensitivity tests) is presented.1979485599
aerobic and anaerobic bacterial flora in semen from fertile and infertile groups of men.semen samples were collected by masturbation under asceptic conditions from men who had sired children within the past 6 mo (group a) and asymptomatic men attending an infertility clinic who had not sired children and whose wives were asymptomatic for infertility (group b). these 109 semen samples were analyzed and cultured for isolation of aerobic and anerobic organisms. overall, 68% of the specimens had positive bacterial cultures: 54% of the samples from group a were positive and 73% from gro ...1979485650
anaerobic bacteria in late infections following orthopedic surgery.twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ...1979492071
recovery of anaerobic bacteria from pediatric patients. a one-year experience.during 1975, 115 speciments from the soft tissues, body fluids, and purulent sites in 105 pediatric ward and nursery patients were submitted to the clinical laboratory for anaerobic study, and 75% of the cultures were positive. in most instance specimens were collected in anaerobic transport tubes containing peptone yeast extract and glucose. in the laboratory, samples were processed by the anaerobic holding jar method. nineteen different anaerobic organisms were isolated; the average number of ...1979495592
osteomyelitis of the mandibular condyle secondary to dental extractions.although osteomyelitis of the mandibular condyle secondary to dental extraction is scarcely mentioned in the medical and dental literature, we have seen three cases within a two-year period. the first two patients were examined for an eroded condyle three months and 18 months following the extractions. the third patient, who was treated one month after extraction for acute osteomyelitis of the condyle, has done well. all three patients required operations and administration of long-term antibiot ...1979503487
obligate anaerobes in clinical veterinary practice.clinical specimens obtained from domestic animals were examined to determine the relative prevalence of obligate anaerobic bacteria and the species represented. of 3,167 samples cultured anaerobically as well as aerobically, 2,234 were bacteriologically positive. of these positive samples, 583 (26%) contained species of obligate anaerobic bacteria in a total of 641 isolates. most positive samples contained anaerobes admixed with aerobic species, although 6% of such samples yielded pure cultures ...1979511987
medium for selective isolation of fusobacterium nucleatum from human periodontal pockets.a selective medium, cve agar, was developed for the isolation of fusobacterium nucleatum from subgingival plaque of periodontally diseased patients. the medium contained 1.0% trypticase (bbl microbiology systems), 0.5% yeast extract, 0.5% nacl, 0.2% glucose, 0.02% l-tryptophan, 1.5% agar, and 5% defibrinated whole sheep blood. erythromycin and crystal violet were added as the selective inhibitory agents at concentrations of 4 and 5 micrograms/ml, respectively. the medium permitted almost total r ...1979521483
differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products.gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ...1979528680
anaerobic isolates in chronic suppurative otitis media. 1979538681
comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline.the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ...1979543353
neonatal scalp abscess and fetal monitoring: factors associated with infection.forty-two of 929 (4.5 per cent) newborn infants prospectively studied following continuous, direct fetal heart rate monitoring during labor developed a scalp abscess at the site of electrode application during the neonatal period. factors associated with infection with the use of a multivariate analysis were duration of monitoring (p less than 0.01) and high-risk indications for monitoring (p less than 0.01). of the 42 infected neonates, 33 had complete bacteriologic studies. microorganisms were ...1977561542
fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon.a total of 154 strains from 22 species of bifidobacterium, peptostreptococcus, lactobacillus, ruminococcus, coprococcus, eubacterium, and fusobacterium, which are present in high concentrations in the human colon, were surveyed for their ability to ferment 21 different complex carbohydrates. plant polysaccharides, including amylose, amylopectin, pectin, polygalacturonate, xylan, laminarin, guar gum, locust bean gum, gum ghatti, gum arabic, and gum tragacanth, were fermented by some strains from ...1977563214
bovine superoxide dismutase and copper ions potentiate the bactericidal effect of autoxidizing cysteine.when cysteine is oxidized by oxygen, hydrogen peroxide is formed, and hydrogen peroxide is very toxic to peptostreptococcus anaerobius vpi 4330-1. native and inactivated superoxide dismutase increased the rate of oxidation of cysteine and thereby potentiated the toxic effect of cysteine. a similar increase in the rate of oxidation of cysteine and in the toxicity of cysteine was obtained with cu2+.1979573589
enhancement of experimental anaerobic infections by blood, hemoglobin, and hemostatic agents.certain foreign materials have been demonstrated to enhance the infectivity of aerobic and anaerobic bacteria. whole blood and other protein compounds encountered in surgical settings or trauma were tested for their effect on infectivity of nonsporeforming anaerobic bacteria. infectious synergistic mixtures of bacteroides fragilis plus peptostreptococcus anaerobius and bacteroides melaninogenicus plus fusobacterium necrophorum were each diluted to a barely noninfectious or minimally infectious c ...1978631883
clindamycin-induced enterocolitis in hamsters.a lethal enterocolitis was induced in hamsters by oral or parenteral administration of clindamycin in amounts comparable to those used in treatment of humans. the intestinal lesions were characterized histologically as an acute inflammatory reaction with pseudomembrane formation and resembled the lesions seen in humans with antibiotic-induced colitis. results of quantitative stool cultures showed the numbers of peptostreptococcus and corynebacterium decreased in animals with colitis after challe ...1978649990
anaerobic microflora of the vagina in children.vaginal cultures from 25 healthy girls from two months to 15 years of age were examined for aerobic, facultatively anaerobic, and obligately anaerobic bacteria. an average of 8.7 species (3.7 aerobic and facultatively anaerobic species; 5.3 obligately anaerobic species) were isolated from these cultures. staphylococcus epidermidis (21), diphtheroids (20), bacteroides (19), peptococci (19), peptostreptococci (14), and bacteroides melaninogenicus (14) were most prevalent organisms.1978686083
nutritional characteristics of megasphaera elsdenii.megasphaera elsdenii b159 (formerly peptostreptococcus elsdenii; rogosa 1971) is able to grow in a chemically defined medium containing minerals, nh4+ as the sole nitrogen source, sulfate as the source of sulfur, acetate and glucose as the carbon and energy sources, and the vitamins biotin, pyridoxine, and calcium pantothenate. when lactate serves as the carbon source, with cysteine present as the reducing agent, acetate is not required. valine and threonine appeared to be required although dire ...1978688105
[various antigenic components in peptococcus and peptostreptococcus strains]. 1978694106
factors related to the oxygen tolerance of anaerobic bacteria.the effect of atmospheric oxygen on the viability of 13 strains of anaerobic bacteria, two strains of facultative bacteria, and one aerobic organism was examined. there were great variations in oxygen tolerance among the bacteria. all facultative bacteria survived more than 72 h of exposure to atmospheric oxygen. the survival time for anaerobes ranged from less than 45 min for peptostreptococcus anaerobius to more than 72 h for two clostridium perfringens strains. an effort was made to relate th ...1978697363
[anaerobiosis in purulent material]. 1978714804
recurrent acute suppurative thyroiditis.two patients had two recurrent episodes each of acute suppurative thyroiditis. circulating concentrations of plasma thyrotrophin, thyroxine, and triiodothyronine were within the range of normal. antibodies to thyroid microsome or thyroglobulin were not found. anaerobic bacteria such as bacteroides, peptostreptococcus, and peptococcus were identified as causative agents, indicating that anaerobic organisms may play an important role in the pathologic process. the present cases are the third and f ...1978717311
comparative susceptibilities of anaerobic bacteria to metronidazole, ornidazole, and sc-28538.the susceptibilities of 284 anaerobic bacteria, including 55 strains of the bacteroides fragilis group, were determined by an agar dilution technique to metronidazole and two newer nitroimidazoles, ornidazole and sc-28538. all three agents showed marked in vitro activity against virtually all anaerobic bacteria tested. at concentrations </=1 mug/ml, sc-28538 was significantly more active than either metronidazole or ornidazole. at concentrations of >1 mug/ml, the activities of all three agents w ...1978718156
bacteriology of chronic otitis media.tympanocentesis was performed in 50 patients with chronic otitis media; pus was cultured aerobically and anaerobically. only aerobes were isolated from 21 patients (42%), three patients had only anaerobes (peptococcus sp), and 25 patients (50%) had both aerobes and anaerobes. only one specimen had no growth. there were 68 aerobic isolates. pseudomonas aeruginosa was recovered in 36 patients (72%); other aerobes commonly recovered included proteus sp and staphylococcus aureus. there were 48 anaer ...1979759663
reconstruction of superior vena cava-right atrial continuity and "anatomic" repair of transposition of great arteries with ventricular septal defect.a superior vena cava-to-right pulmonary artery anastomosis (glenn procedure), used to palliate certain types of cyanotic congenital heart defects, has usually been left intact at the time of definitive intracardiac repair. late failure of this shunt has prompted attempts to reestablish superior vena cava-to-right atrium and right ventricle-to-right pulmonary artery continuity. the present patient, a 10-year-old boy with transposition of the great arteries, ventricular septal defect, and mild pul ...1976768645
medium-dependent inhibition of peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media.of 13 species of anaerobic cocci, peptostreptococcus anaerobius was the only species tested that was sensitive to 0.1% sodium polyanetholsulfonate (sps). however, the sensitivity of p. anaerobius to sps varied according to the media in which the cultures were grown. in supplemented peptone (b-d) and brain heart infusion media, most strain of p. anaerobius were not inhibited by sps. gelatin and proteose peptone were the medium components which were protective. the minimal inhibitory concentration ...1976770497
a bacteriological study of the intestinal mucosa and luminal fluid of adults with acute diarrhoea.bacteriological studies of jejunal mucosal biopsy specimens and contents were performed on 22 hospitalized adult patients with acute diarrhoea and 24 control normal subjects. none of the washed homogenates of the mucosal specimens were sterile and only one fluid specimen obtained from a control subject was sterile. a definite enteric pathogen was found in only five of the 22 diarrhoea patients. there was no qualitative difference in the bacterial profile of the jejunal mucosa and contents of the ...1976779681
polymicrobial etiology of acute pelvic inflammatory disease.we studied 204 women with acute pelvic inflammatory disease to delineate further the causes of that illness. gonococci were recovered from 91. gonococcal pili antibody rose or fell significantly in 12 of 18 patients with positive cultures and only two of 19 who had negative cultures and smears for neisseria gonorrhaoea(p smaller than 0.005). n. gonorrhoeae was found in peritoneal exudate from eight of 21 patients with, and none of 33 without, cervical gonococcal infection. among patients with se ...1975806017
prevalence and antibiotic susceptibility of organisms isolated from acute soft-tissue abscesses secondary to dental caries. 1975806046
ticarcillin disodium in anaerobic infections.twenty-five patients were treated with ticarcillin disodium, 18 of whom had anaerobic infections that included pleuropulmonary infections (seven), mandibular osteomyelitis (four), perirectal abscess (two), sepsis, primary site unknown (one), liver abscess (one), pelvic abscess (one), decubitus ulcer (one), and synergistic gangrene (one). seven had no anaerobic infections. three had anaerobic septicemia. culture results included anaerobes: peptococci (ten), peptostreptococci (ten), bacteroides fr ...1978718311
bacterial arthritis in the adult.a brief review of bacterial arthritis as seen at the mayo clinic in the years 1958 through 1973 has been presented with a selected literature review. once considered a disease of children and adolescents, this disease now is seen more often in adults, especially in those who are elderly, are chronically ill, or are receiving medications that lower the host resistance in invading bacteria. the diagnosis must be made early, with rapid isolation and identification of the organism. proper antibacter ...1975809738
anaerobic bacteremia as observed in a children's hospital. clinically this may signify true anaerobic ascertain the significance of anaerobic bacteremia in a children's hospital, the records of all patients whose blood cultures grew anaerobes during a 24-month period were reviewed. anaerobes were isolated from 144 out of 1,126 blood cultures yielding bacteria. anaerobic diphtheroid grew in 122 out of 143 anaerobic cultures, but only 4 out of 122 were isolated from patients with anaerobic sepsis. nine per cent of the total episodes of anaerobic bacteremia occurred in 13 children who met out cr ...1978679583
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