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assessment of pediococcus acidilactici as a potential silage inoculant.eighteen pediococcus strains were screened for their potential as silage inoculants. pediococcus acidilactici g24 was found to be the most suitable, exhibiting a short lag phase on both glucose and fructose, a rapid rate of acid production, a high sugar-to-lactate conversion efficiency, no detectable breakdown of proteins or lactic acid, and the ability to grow within a broad range of ph and temperature. when tested in laboratory silos using grass with a water-soluble carbohydrate content of 24 ...199216348773
digestion of herring by indigenous bacteria in the minke whale forestomach.northeastern atlantic minke whales (balaenoptera acutorostrata) have a multichambered stomach system which includes a nonglandular forestomach resembling that of ruminants. bacteria from the forestomachs of herring-eating whales were enumerated and isolated in an anaerobic rumen-like culture medium (m8w medium). the total viable population of anaerobic bacteria ranged from 73 x 10 to 145 x 10/ml of forestomach fluid (n = 4). lactobacillus spp. (19.7%), streptococcus spp. (35.9%), and ruminococcu ...199416349460
pure culture fermentation of brined cucumbers.the relative abilities of pediococcus cerevisiae, lactobacillus plantarum, l. brevis, and several other species of lactic acid bacteria to grow and produce acid in brined cucumbers were evaluated in pure culture fermentations. such fermentations were made possibly by the use of two techniques, gamma radiation (0.83 to 1.00 mrad) and hot-water blanching (66 to 80 c for 5 min), designed first to rid the cucumbers of naturally occurring, interfering, and competitive microbial groups prior to brinin ...196416349651
pure culture fermentation of green olives.the method previously developed by us for the pure-culture fermentation of brined cucumbers and other vegetables has been applied successfully to manzanillo variety olives. field-run grade fruit was processed first by conventional procedures to remove most of the bitterness. then the relative abilities of lactobacillus plantarum, l. brevis, pediococcus cerevisiae, and leuconostoc mesenteroides to become established and produce acid in both heat-shocked (74 c for 3 min) and unheated olives, brine ...196616349674
occurrence of an inhibitor of lactic acid bacteria in green olives.green olives were found to contain an inhibitor(s) of several species of lactic acid bacteria usually associated with the spanish-type brined olive fermentation. the inhibitor was demonstrated by the presence of inhibition zones surrounding tissue which had been cut from frozen olives and implanted in a seeded nutritive agar medium. relative potencies of aqueous extracts of frozen olives were determined by a paper disc assay method. the mission variety of olive contained the most inhibitor, and ...196716349729
bloater formation by gas-forming lactic acid bacteria in cucumber fermentations.the formation of "bloaters" (hollow stock) in cucumbers brined for salt-stock purposes at 5 to 10% salt has been associated with gaseous fermentation caused chiefly by yeasts. recently, serious early bloater damage, not attributable to yeasts, has been observed in commercial-scale experiments on control of bloaters in overnight dill pickles brined in 50-gal barrels at 3.0 to 4.5% salt. growth of fermentative species of yeasts was effectively controlled by the addition of 0.025, 0.05, and 0.1% so ...196816349808
fermentation of philippine vegetable blends.seven blends of philipphine vegetables, two of which contained soybeans and one mongo bean sprouts, were prepared for fermentation and study of microbiological and chemical changes. the fermentations were typical lactic acid bacterial fermentations, initiated by leuconostoc mesenteroides and continued by lactobacillus brevis, pediococcus cerevisiae, and l. plantarum. the combination of high acidity and low ph resembled other vegetable fermentations, such as sauerkraut. the procedure offers a met ...196916349831
microbial inhibition by an isolate of pediococcus from cucumber brines.we reported earlier that pediococcus cerevisiae fbb-61 inhibited lactobacillus plantarum fbb-67 in mixed species inoculation used for the fermentation of brined cucumbers. herein, 16 isolates of the pediococcus genus from various sources were tested for inhibitory activity against l. plantarum and other microorganisms by a seeded-agar screening technique. only two of the 16 isolates gave consistent and distinctive zones of inhibition, and both were isolated from fermenting cucumber brines on sep ...197516350041
pediococcus parvulus gtf gene encoding the gtf glycosyltransferase and its application for specific pcr detection of beta-d-glucan-producing bacteria in foods and beverages.exopolysaccharide production by lactic acid bacteria is beneficial in the dairy and oat-based food industries and is used to improve the texture of the fermented products. however, beta-d-glucan-producing bacteria are considered spoilage microorganisms in alcoholic beverages because their secreted exopolysaccharides alter the viscosity of cider, wine, and beer, rendering them unpalatable. the plasmidic glycosyltransferase (gtf) gene of the pediococcus parvulus 2.6 strain isolated from ropy cider ...200616416914
pediococcus stilesii sp. nov., isolated from maize grains.a gram-positive, coccus-shaped, lactic acid bacterium, strain lmg 23082t, was isolated from steeped maize grains. the organism is homofermentative and produces d- and l-lactic acid from glucose. 16s rrna gene sequence analysis revealed that the organism belongs to the genus pediococcus, with pediococcus pentosaceus and pediococcus acidilactici as nearest neighbours. genotypic fingerprinting, whole-cell protein electrophoresis, dna-dna hybridizations and physiological and biochemical tests allowe ...200616449434
ladr, a new padr-related transcriptional regulator from listeria monocytogenes, negatively regulates the expression of the multidrug efflux pump mdrl.the listeria monocytogenes genome encodes putative multidrug efflux transporters but only the mdrl transporter has been partially characterized in the wild-type lo28 strain. here, we show in the lo28 strain, that the expression of mdrl is repressed at the transcriptional level, under standard growth conditions, by the product of a new gene ladr (lmo1408), and the expression of mdrl is induced in the presence of rhodamine. phylogenetic analysis in related firmicutes shows that ladr, conserved in ...200616451184
antibiotic susceptibility patterns and resistance genes of starter cultures and probiotic bacteria used in food.a survey of starter and probiotic cultures was carried out to determine the current antibiotic resistance situation in microbial food additives in switzerland. two hundred isolates from 90 different sources were typed by molecular and other methods to belong to the genera lactobacillus (74 samples), staphylococcus (33 samples), bifidobacterium (6 samples), pediococcus (5 samples), or were categorized as lactococci or streptococci (82 samples). they were screened for phenotypic resistances to 20 ...200616464696
growth response and modifications of organic nitrogen compounds in pure and mixed cultures of lactic acid bacteria from wine.the interactions between the proteolytic x2l strain of oenococcus oeni and the non-proteolytic 12p strain of pediococcus pentosaceus were assayed. the characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. o. oeni showed poor cell growth and greater ability in the release of amino acids to the extracellular medium, whereas p. pentosaceus showed a higher yield in cell production with a decrease in the amino acid c ...200616467990
effects of a bacterial hay preservative (pediococcus pentosaceus) on hay under experimental storage conditions.the abundant growth of molds and thermophilic actinomycetes in stored hay decreases its quality and can be hazardous for the producer who inhales these contaminants when the moldy hay is fed in closed barns. these microbes are responsible for a respiratory disease called farmer's lung. products, including bacterial cultures that can be inoculated in hay, are available to prevent hay deterioration by molds and bacteria. the aim of this study was to verify the effectiveness of pediococcus pentosac ...199516535180
development and characterization of lactose-positive pediococcus species for milk fermentation.bacteriophages against streptococcus thermophilus are a growing problem in the italian cheese industry. one possible control method involves replacing s. thermophilus in mozzarella starter blends with lactic acid bacteria from a different genus or species. in this study, we evaluated lactose-positive pediococci for this application. because we could not identify any commercially available pediococci with fast acid-producing ability in milk, we transformed pediococcus pentosaceus atcc 25744, p. p ...199616535280
vegetable-associated pediococcus parvulus produces pediocin pa-1.two bacteriocin-producing strains of pediococcus parvulus were isolated from minimally processed vegetables. recombinant dna techniques revealed the presence of the pediocin pa-1 gene in both strains. biochemical analysis confirmed the production of pediocin pa-1 and excluded the presence of other bacteriocins.199716535615
usefulness of length heterogeneity-pcr for monitoring lactic acid bacteria succession during maize ensiling.the use of length-heterogeneity pcr was explored to monitor lactic acid bacteria succession during ensiling of maize. bacterial diversity was studied during the fermentation of 30-day-old maize in optimal and spoilage-simulating conditions. a length heterogeneity pcr profile database of lactic acid bacteria isolated from the silage and identified by 16s rrna gene sequencing was established. although interoperonic 16s rrna gene length polymorphisms were detected in some isolates, strain analysis ...200616542413
the effect of prebiotics on production of antimicrobial compounds, resistance to growth at low ph and in the presence of bile, and adhesion of probiotic cells to intestinal mucus.screening of five bile salt-resistant and low ph-tolerant lactic acid bacteria for inhibitory activity against lactic acid bacteria and bacterial strains isolated from the faeces of children with hiv/aids. determining the effect of prebiotics and soy milk-base on cell viability and adhesion of cells to intestinal mucus.200616553737
reversal of d-cycloserine inhibition of bacterial growth by alanine.zygmunt, walter a. (mead johnson & co., evansville, ind.). reversal of d-cycloserine inhibition of bacterial growth by alanine. j. bacteriol. 84:154-156. 1962.-reversal of the antibacterial activity of d-4-amino-3-isoxazolidone by alanine in bacterial cultures actively growing on chemically defined media was compared in cultures requiring exogenous alanine and those capable of its synthesis. dl-alanine was the most effective reversal agent in pediococcus cerevisiae, an alanine-requiring organism ...196216561951
oxidative metabolism in pediococcus pentosaceus iii. glucose dehydrogenase system.lee, chin k. (north carolina state of the university of north carolina, raleigh), and walter j. dobrogosz. oxidative metabolism in pediococcus pentosaceus. iii. glucose dehydrogenase system. j. bacteriol. 90:653-660. 1965.-a method was developed for the purification of glucose dehydrogenase from pediococcus pentosaceus az-25-5. the procedures included treatments with protamine sulfate, ammonium sulfate, and heat in addition to acid precipitation, calcium phosphate adsorption and elution, and die ...196516562063
probiotics for animal nutrition in the european union. regulation and safety assessment.probiotics are alive micro-organisms, generally bacteria but also yeasts than, when ingested alive in sufficient amount, they have a positive effect on the health going beyond the nutritional ones commonly known. probiotics may operate through a nutritional and/or health or sanitary effect. micro-organisms used in animal feed in the eu are mainly bacterial strains of gram-positive bacteria belonging to the types bacillus, enterococcus, lactobacillus, pediococcus, streptococcus and strains of yea ...200616563585
characterization of hora and its flanking regions of pediococcus damnosus abbc478 and development of more specific and sensitive hora pcr method.to characterize hora and its flanking regions of pediococcus damnosus abbc478 and, on the basis of this insight, to develop a more specific and sensitive hora pcr method.200616599994
nonstarter lactic acid bacteria biofilms and calcium lactate crystals in cheddar cheese.a sanitized cheese plant was swabbed for the presence of nonstarter lactic acid bacteria (nslab) biofilms. swabs were analyzed to determine the sources and microorganisms responsible for contamination. in pilot plant experiments, cheese vats filled with standard cheese milk (lactose:protein = 1.47) and ultrafiltered cheese milk (lactose:protein = 1.23) were inoculated with lactococcus lactis ssp. cremoris starter culture (8 log cfu/ml) with or without lactobacillus curvatus or pediococci acidila ...200616606716
gluten breakdown by lactobacilli and pediococci strains isolated from sourdough.to evaluate the growth and metabolic activity of lactobacilli and pediococci strains in a gluten base medium (gbm), formulated for a proper selection of proteolytic strains to be used in sourdough fermentation.200616620203
rpob gene: a target for identification of lab cocci by pcr-dgge and melting curves analyses in real time pcr.lactic acid bacteria (lab) are essential in the quality of many fermented beverages like beer, cider and wine. in the two later cases, they convert malic acid into lactic acid during the malolactic fermentation. after fermentation, microbial stabilization is needed to prevent the development of spoilage bacteria species. among them, cocci lead to different alterations: pediococcus sp., and some strains of leuconostoc mesenteroides and oenococcus oeni can produce exopolysaccharides which modify w ...200616626824
fermentation of wheat: effects of backslopping different proportions of pre-fermented wheat on the microbial and chemical composition.the objective of the study was to examine effect of backslop on the chemical and microbiological characteristics of fermented wheat (fw). coarsely ground wheat was mixed with water (1:3 wt/wt) and inoculated with 6 log cfu ml(-1) each of an overnight culture of lactobacillus plantarum and pediococcus pentosaceus. four fermentation treatments were conducted in 45 1, closed, pvc containers over 48 hours. three treatments investigated the benefits of the addition of previously fermented wheat (back ...200616649578
microspectrofluorometric measurement of chloroplast dna in dividing and expanding leaf cells of spinacia oleracea.absolute dna amounts of individual chloroplasts from mesophyll and epidermal cells of developing spinach leaves were measured by microspectrofluorometry using the dna-specific stain, 4,6-diamidino-2-phenyl indole, and the bacterium, pediococcus damnosus, as an internal standard. values obtained by this method showed that dna amounts of individual chloroplasts from mesophyll cells fell within a normal distribution curve, although mean dna amounts changed during leaf development and also differed ...198616664887
elimination of escherichia coli o157:h7 from fermented dry sausages at an organoleptically acceptable level of microencapsulated allyl isothiocyanate.four sausage batters (17.59% beef, 60.67% pork, and 17.59% pork fat) were inoculated with two commercial starter culture organisms (>7 log(10) cfu/g pediococcus pentosaceus and 6 log(10) cfu/g staphylococcus carnosus) and a five-strain cocktail of nonpathogenic variants of escherichia coli o157:h7 to yield 6 to 7 log(10) cfu/g. microencapsulated allyl isothiocyanate (ait) was added to three batters at 500, 750, or 1,000 ppm to determine its antimicrobial effects. for sensory analysis, separate b ...200616672446
development of a competitive exclusion product for poultry meeting the regulatory requirements for registration in the european union.competitive exclusion treatment is able to increase the pathogen colonization resistance of day-old chicks by applying probiotic bacteria stabilizing the indigenous microflora. in order to develop a safe microbial feed additive, various bacterial strains were isolated out of the gastrointestinal tract of healthy chickens. one hundred twenty-one representatives were selected based on differences in whole-cell protein patterns and screened for antagonistic properties. five effective strains (pedio ...200616676374
plasmid profile patterns and properties of pediococci isolated from caper fermentations.a collection comprising 14 isolates of pediococcus pentosaceus and one pediococcus acidilactici from the fermentation of caper fruits was studied. all isolates showed very similar fermentation profiles and produced a limited number of exoenzymes. all isolates carried large plasmids of diverse sizes between 20 and 55 kb, while some also contained smaller plasmids between 10 and 16 kb. cluster analysis of plasmid profiles revealed four main groups with various degrees of similarities. all amino ac ...200616715824
lactobacillus plantarum and pediococcus pentosaceus starter cultures as a tool for microflora management in malting and for enhancement of malt processability.lactobacillus plantarum vtt e-78076 (e76) and pediococcus pentosaceus vtt e-90390 (e390) starter cultures were added to the steeping water of normal malting barley in order to balance the microbial community and to enhance malt processability. in this study, we also investigated the effects of lactic acid-acidified mrs-spent medium (mrs-la) on malting performance. malting trials with five different two-row barley varieties were carried out in 25 kg pilot scale. the starter cultures promoted yeas ...200616719505
lactobacillus plantarum gene clusters encoding putative cell-surface protein complexes for carbohydrate utilization are conserved in specific gram-positive bacteria.genomes of gram-positive bacteria encode many putative cell-surface proteins, of which the majority has no known function. from the rapidly increasing number of available genome sequences it has become apparent that many cell-surface proteins are conserved, and frequently encoded in gene clusters or operons, suggesting common functions, and interactions of multiple components.200616723015
microscopic and voltammetric characterization of bioanalytical platforms based on lactate oxidase.a microscopic and voltammetric characterization of lactate oxidase- (lox-) based bioanalytical platforms for biosensor applications is presented. in this context, emphasis is placed on amperometric biosensors based on lox that have been immobilized by direct absorption on carbon surfaces, in particular, glassy carbon (gc) and highly ordered pyrolytic graphite (hopg). the immobilized lox layers have been characterized using atomic force microscopy (afm) under liquid conditions and cyclic voltamme ...200616732675
data mining and characterization of a novel pediocin-like bacteriocin system from the genome of pediococcus pentosaceus atcc 25745.the genome of pediococcus pentosaceus atcc 25745 contains a gene cluster that resembles a regulated bacteriocin system. the gene cluster has an operon-like structure consisting of a putative pediocin-like bacteriocin gene (termed pena) and a potential immunity gene (termed peia). genetic determinants involved in bacteriocin transport and regulation are also found in proximity to pena and peia but the so-called accessory gene involved in transport and the inducer gene involved in regulation are m ...200616735728
biofilm formation in an ice cream plant.the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ...200616779628
histamine, histidine, and growth-phase mediated regulation of the histidine decarboxylase gene in lactic acid bacteria isolated from wine.fermented foods are frequently contaminated by histamine that is generated by microorganisms with histidine decarboxylase activity. the ingestion of large amounts of histamine can cause serious toxicological problems in humans. a study of the effects of histamine, histidine, and growth phase on histamine production by lactic acid bacteria isolated from wine is reported here. with northern blots and specific activity analysis, we observed that histidine induces the expression of the histidine dec ...200616790022
environmental stress response in wine lactic acid bacteria: beyond bacillus subtilis.lactic acid bacteria (lab) constitute a heterogeneous group of bacteria that are traditionally used to produce fermented foods. the industrialization of food transformations has increased the economical importance of lab, as they play a crucial role in the development of the organoleptic and hygienic quality of fermented products. however, the strains selected for industrial purposes, should tolerate adverse conditions encountered in industrial processes, either during starter handling and stora ...200616809231
characterisation of prototype nurmi cultures using culture-based microbiological techniques and pcr-dgge.undefined nurmi-type cultures (ntcs) have been used successfully to prevent salmonella colonisation in poultry for decades. such cultures are derived from the caecal contents of specific-pathogen-free birds and are administered via drinking water or spray application onto eggs in the hatchery. these cultures consist of many non-culturable and obligately anaerobic bacteria. due to their undefined nature it is difficult to obtain approval from regulatory agencies to use these preparations as direc ...200616814892
diversity of a stable enrichment culture which is useful for silage inoculant and its succession in alfalfa silage.alfalfa is a kind of forage that is difficult to ensile for good quality. therefore, inoculants are always used to enhance the preservation of alfalfa silage. through continuous restricted subcultivation, a lactic acid bacteria community (al2) was selected from well-fermented alfalfa silage, which sharply decreased the ph level and produced a large amount of lactic acid. the adding of al2 to alfalfa at ensiling resulted in a more rapid drop in ph and higher levels of lactic acid, and it also red ...200616819954
isolation and characterization of lactic acid bacteria from suan-tsai (fermented mustard), a traditional fermented food in taiwan.to isolate, characterize, and identify lactic acid bacteria (lab) in suan-tsai (fermented mustard), a traditional fermented food in taiwan.200616834599
environmental factors influencing growth of and exopolysaccharide formation by pediococcus parvulus 2.6.natural exopolysaccharides (epss) from food-grade lactic acid bacteria have potential for development and exploitation as food additives and functional food ingredients with both health and economic benefits. in this study, we have examined the physiological capacity of eps production in pediococcus parvulus 2.6. eps formation by p. parvulus 2.6 was found to be linked to biomass yields, provided that glucose was not limiting. higher biomass yields and eps productions were obtained when cultures ...200616854485
methylglyoxal bypass identified as source of chiral contamination in l(+) and d(-)-lactate fermentations by recombinant escherichia coli.two new strains of escherichia coli b were engineered for the production of lactate with no detectable chiral impurity. all chiral impurities were eliminated by deleting the synthase gene (msga) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both l: (+)- and d: (-)-lactate. strain tg113 contains only native genes and produced optically pure d: (-)-lactate. strain tg108 contains the ldhl gene from pediococcus acidilactici and produced only l: (+)-lactate. in mineral sa ...200616868860
isolation and characterization of lactic acid bacteria from dochi (fermented black beans), a traditional fermented food in taiwan.to isolate, characterize and identify lactic acid bacteria (lab) in dochi (fermented black beans), a traditional fermented food in taiwan.200616869910
short-chain fatty acid formation in the hindgut of rats fed native and fermented oat fibre concentrates.the formation of scfa in rats fed fermented oat fibre concentrates was compared with that of rats fed native oat fibre concentrate. the cultures used were lactic acid bacteria consisting of lactobacillus bulgaricus and streptococcus thermophilus (v2), the exopolysaccharide-producing strain pediococcus damnosus 2.6 (pd) and l. reuteri (lr). the materials were incorporated into test diets yielding a concentration of indigestible carbohydrates of 80 g/kg (dry weight). rats fed the v2-fermented fibr ...200616869990
detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animals.the production of antimicrobial activities as well as the presence of bacteriocin structural genes (enta, entb, entp, entq, cyll, entas-48, bac31, and entl50a/b) were studied in 140 non-selected faecal enterococcal isolates recovered from wild animals. eight different indicator strains (including listeria monocytogenes, pediococcus pentosaceus, and different enterococcal species) were used for antimicrobial activity detection. twenty-five of the 140 enterococci (18%) showed antimicrobial activit ...200716872815
assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine.susceptibility to 12 antibiotics was tested in 75 unrelated lactic acid bacteria strains of wine origin of the following species: 38 lactobacillus plantarum, 3 lactobacillus hilgardii, 2 lactobacillus paracasei, 1 lactobacillus sp, 21 oenococcus oeni, 4 pediococcus pentosaceus, 2 pediococcus parvulus, 1 pediococcus acidilactici, and 3 leuconostoc mesenteroides. the minimal inhibitory concentrations of the different antibiotics that inhibited 50% of the strains of the lactobacillus, leuconostoc a ...200616876896
microbiological and sensory quality of dry fermented sausages containing alginate-microencapsulated lactobacillus reuteri.the probiotic bacterium lactobacillus reuteri was added to dry sausage batter, without or after being microencapsulated in alginate using either extrusion or emulsion technology. pediococcus pentosaceus and staphylococcus carnosus were added at 7 log cfu/g as starter cultures for fermentation. the sausage batter was stuffed in 55 mm fibrous casings and fermented, with smoking, at <or=26 degrees c and 88% relative humidity (rh) for 72 h. during fermentation as well as drying at 75% rh and 13 degr ...200616884803
yeast and lactic acid flora of tej, an indigenous ethiopian honey wine: variations within and between production units.a total of 200 samples of tej, an indigenous ethiopian honey wine, were collected from ten production units at different production times. the samples were analysed for their microbial flora. mean counts of aerobic mesophilic bacteria and aerobic spores for the different production units were <3 log cfu/ml. coliforms and other members of enterobacteriaceae were below detectable levels basically due to the low ph of the samples (<4.0). yeasts were among the dominant micro-organisms in all samples ...200616943014
survival of escherichia coli o157:h7 in dry fermented sausages containing micro-encapsulated probiotic lactic acid bacteria.escherichia coli o157:h7 is capable of surviving the rigorous processing steps during the manufacture of dry fermented sausages. the effect of adding two probiotic organisms, lactobacillus reuteri and bifidobacterium longum as co-cultures with the meat starter cultures pediococcus pentosaceus and staphylococcus carnosus on the viability of e. coli o157:h7 in dry fermented sausages was studied. a 5 strain cocktail of e. coli o157:h7 was added at 7.4 log cfu/g to the sausage batter and challenged ...200716943098
the effects of lactobacillus buchneri 40788 and pediococcus pentosaceus r1094 on the fermentation of corn silage.the effect of inoculating whole-plant corn at the time of harvest with lactobacillus buchneri 40788 (4 x 10(5) cfu/g of fresh forage) combined with pediococcus pentosaceus r1094 (1 x 10(5) cfu/g) on the fermentation and aerobic stability of corn silage (37% dry matter) through 361 d of ensiling was investigated. dry matter recovery was similar between treatments throughout the study except at one early time point (14 d), when treated silage had a lower recovery than untreated silage. the concent ...200616960076
benefits of a synbiotic formula (synbiotic 2000forte) in critically ill trauma patients: early results of a randomized controlled trial.since probiotics are considered to exert beneficial health effects by enhancing the host's immune response, we investigated the benefits of a synbiotics treatment on the rate of infections, systemic inflammatory response syndrome (sirs), severe sepsis, and mortality in critically ill, mechanically ventilated, multiple trauma patients. length of stay in the intensive care unit (icu) and number of days under mechanical ventilation were also evaluated.200616983476
lethality of commercial whole-muscle beef jerky manufacturing processes against salmonella serovars and escherichia coli o157:h7.thermal processes used in making whole-muscle beef jerky include a drying step, which may result in enhanced pathogen thermotolerance and evaporative cooling that reduce process lethality. several salmonellosis outbreaks have been associated with beef jerky. in this study, a standardized process was used to inoculate beef strips with five-strain cocktails of either salmonella serovars or escherichia coli o157:h7, to marinate the strips at ph 5.3 for 22 to 24 h at 5 degrees c, and to convert the ...200616995510
pediococcus ethanolidurans sp. nov., isolated from the walls of a distilled-spirit-fermenting cellar.two novel pediococcus strains, z-9(t) and z-17, were isolated from a distilled-spirit-fermenting cellar in hebei province, china. the cells were gram-positive, non-spore-forming, non-motile cocci and occurred in pairs or tetrads. the strains were facultatively anaerobic and produced only lactic acid [d(-) and l(+) isomers in the ratio 50 : 50] from glucose fermentation. catalase activity was not present. both strains were able to grow in 6.5 % ethanol and at ph 3.5, but not in 4 % nacl. the mean ...200617012570
determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor "indicator microorganism".successful application of growth inhibition techniques for quantitative determination of bacteriocins relies on the sensitivity of the applied indicator microorganism to the bacteriocin to which is exposed. however, information on indicator microorganisms' performance and comparisons in bacteriocin determination with bioassays is almost non-existing in the literature. the aim of the present work was to evaluate the parameter "indicator microorganism" in bioassays carried out on solid -agar diffu ...200617032438
antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms.non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ...200717049799
fluorescence in situ hybridisation detection of lactobacillus plantarum group on olives to be used in natural fermentations.at present there are very few studies on the bacterial diversity of olives and on the importance of the microbial species for the fermentation of olives aimed to table olives production. most of the authors report on the occurrence of lactobacillus plantarum as principal member of these communities or at least as the species responsible for the fermentation. in this study, fluorescence in situ hybridisation (fish) with 16s rrna probes was used to evaluate the occurrence of l. plantarum in olives ...200617052794
modification of the data-processing method for the turbidimetric bioassay of nisin.the data processing method of the turbidimetric bioassay of nisin was modified to facilitate its industrial application. the influence of the initial indicator concentration was minimized by a redefined specific dose of the bacteriocin as the quotient between the titer of the added bacteriocin and the initial population density of the indicator in the suspension. it was found that dc = 0.125 microg ml(-1) was the critical dose of nisin that can cause a complete inhibition of the indicator, pedio ...200717063329
isolation and identification of a paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin.a new bacterial strain, displaying potent antimicrobial properties against gram-negative and gram-positive pathogenic bacteria, was isolated from food. based on its phenotypical and biochemical properties as well as its 16s rrna gene sequence, the bacterium was identified as paenibacillus polymyxa and it was designated as strain osy-df. the antimicrobials produced by this strain were isolated from the fermentation broth and subsequently analyzed by liquid chromatography-mass spectrometry. two an ...200717071789
antibiotic resistances of starter and probiotic strains of lactic acid bacteria.the antibiotic resistances of 45 lactic acid bacteria strains belonging to the genera lactobacillus, streptococcus, lactococcus, pediococcus, and leuconostoc were investigated. the objective was to determine antibiotic resistances and to verify these at the genetic level, as is currently suggested by the european "qualified presumption of safety" safety evaluation system for industrial starter strains. in addition, we sought to pinpoint possible problems in resistance determinations. primers wer ...200717122388
rna-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samples.recently we showed the applicability and sensitivity of the rna-based sandwich hybridisation assay (sha) for detection of gram-negative cells in environmental samples [leskelä, t., tilsala-timisjärvi, a., kusnetsov, j., neubauer, p., breitenstein, a., 2005. sensitive genus-specific detection of legionella by a 16s rrna based sandwich hybridization assay. j. microbiol. met. 62, 167-179.]. in this study the aim was to test and optimise this method for the detection of gram-positive cells from brew ...200717157400
new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria.the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ...200717173232
glucan and fructan production by sourdough weissella cibaria and lactobacillus plantarum.after a large screening on sourdough lactic acid bacteria, exopolysaccharide (eps)-forming strains of weissella cibaria, lactobacillus plantarum, and pediococcus pentosaceus were selected. after 6 days of incubation at 30 degrees c, the synthesis of eps in mrs-based broth ranged from 5.54 to 7.88 mg ml-1. eps had an apparent molecular mass of ca. 104 da. as shown by carbohydrate consumption, the synthesis of eps was found from sucrose only. two types of homopolysaccharides were synthesized: gluc ...200617177514
influence of pediococcus-based probiotic on coccidiosis in broiler chickens.coccidiosis is the major parasitic disease of poultry and is caused by the apicomplexan parasites eimeria. drugs and live vaccines are the 2 main control measures of the disease; however, due to increasing concerns with prophylactic drug use and the high cost of vaccines, alternative control methods are needed. recent evidence that various dietary and live microbial supplements can influence host immunity against enteric diseases prompted us to investigate the role of a pediococcus-based probiot ...200717179417
partial characterization of bacteriocins produced by human lactococcus lactis and pediococccus acidilactici isolates.the aim of this study was to isolate bacteriocin-producing lactic acid bacteria (lab) from human intestine.200717184344
biodiversity of bacterial ecosystems in traditional egyptian domiati cheese.bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ...200717189434
vanillin production from simple phenols by wine-associated lactic acid bacteria.the ability of lactic acid bacteria (lab) to metabolize certain phenolic precursors to vanillin was investigated.200717209816
daptomycin susceptibility of unusual gram-positive bacteria: comparison of results obtained by the etest and the broth microdilution method.mics of daptomycin, linezolid, and vancomycin against 212 isolates, including listeria monocytogenes and pediococcus, leuconostoc, rhodococcus, and nocardia spp., were determined by the broth microdilution method; daptomycin mics were also determined by the etest. except with those for leuconostoc spp., daptomycin etest mics showed >90% agreement with mics obtained by the broth microdilution method.200717220420
evaluation of the efficacy of a probiotic containing lactobacillus, bifidobacterium, enterococcus, and pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities.the aim of this work was to investigate the efficacy of a new multibacterial species probiotic in broiler nutrition. the probiotic contained 2 lactobacillus strains, 1 bifidobacterium strain, 1 enterococcus strain, and 1 pediococcus strain. four hundred 1-d-old male cobb broilers were allocated in 4 experimental treatments for 6 wk. the experimental treatments received a corn-soybean basal diet and were as follows: "control," with no other additions; "probiotic in feed and water," (pfw) with pro ...200717234844
inventory and monitoring of wine microbial consortia.the evolution of the wine microbial ecosystem is generally restricted to saccharomyces cerevisiae and oenococcus oeni, which are the two main agents in the transformation of grape must into wine by acting during alcoholic and malolactic fermentation, respectively. but others species like the yeast brettanomyces bruxellensis and certain ropy strains of pediococcus parvulus can spoil the wine. the aim of this study was to address the composition of the system more precisely, identifying other comp ...200717235561
screening and selection of lactic acid bacteria strains suitable for ensiling grass.lactic acid bacteria (lab) strains shown to have broad-spectrum antimicrobial activity were screened for potential as grass silage inoculants. the strains capable of rapidly lowering the ph of the grass matrix and with low proteolytic activity were assessed in laboratory-scale silos in a grass matrix containing natural microbial flora.200717241337
susceptibility of pediococcus spp. to antimicrobial agents.to investigate the susceptibility of pediococcus species to antimicrobial agents.200717241343
limitations in the use of drosophila melanogaster as a model host for gram-positive bacterial infection.to examine sensitivities of various drosophila melanogaster strains towards human pathogenic and nonpathogenic gram-positive bacteria.200717257264
nanomechanical properties of globular proteins: lactate oxidase.we report on the study of the nanomechanical properties of a lactate oxidase (lox) monolayer immobilized on gold substrates by atomic force microscopy techniques operating under buffer conditions. topographical contact mode imaging evidenced the protein deformation under the applied tip load. we performed approaching force curves with both stiff and soft cantilevers by imposing maximum loads of 1.6 nn and 400 pn, respectively. we found that the experimental data were well fitted by the hertz mod ...200717261045
a five-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged with salmonella enterica serovar typhimurium.salmonella spp. infection is a major cause of gastroenteritis, with many thousands of cases reported in the european union every year. the use of probiotics offers the potential to improve this situation. here, we investigate the effects of oral treatment of pigs with a defined lactic acid bacteria culture mixture on both clinical and microbiological signs of salmonella enterica serovar typhimurium infection. fifteen weaned pigs blocked by sex and weight were administered control milk or a mixtu ...200717261517
optimization of technical conditions for the transformation of pediococcus acidilactici p60 by electroporation.previously reported techniques for the electrotransfer of foreign dna into pediococci yield only a small number of transformants/mug dna, especially when using undomesticated strains. this study reports an improved protocol for the electrotransformation of pediococci, based on trials using pediococcus acidilactici p60 and the plasmid prs4c1. the improved protocol yields from 2 to 3 log units more transformants than the previously reported methods, with up to (9.1+/-1.3)x10(4) transformants/mug o ...200717275085
influence of the carbohydrate source on beta-glucan production and enzyme activities involved in sugar metabolism in pediococcus parvulus 2.6.the influence of carbohydrate source on growth, exopolysaccharide (eps) production and on the activity of the enzymes implicated in energy generation and udp-glucose synthesis in pediococcus parvulus 2.6 was evaluated. the highest eps production was obtained on glucose, while fructose was a poor substrate for eps synthesis. hplc and nmr analysis on monomer composition and structure of the eps showed that this strain produced the same beta-glucan, regardless of the carbohydrate source. the alpha- ...200717303279
antimicrobial activity of nisin against oenococcus oeni and other wine bacteria.nisin is a bacteriocin used against food spoilage bacteria. sulphur dioxide is a potent antioxidant as well as an antimicrobial agent widely used in the wine industry. in this study we describe the effect of these important antibacterial agents on the growth of a collection of 64 lactic acid bacteria (23 oenococcus, 29 lactobacillus, 3 leuconostoc and 9 pediococcus), 23 acetic acid bacteria and 20 yeast isolates, most of them recovered from wine. minimal inhibitory concentrations (mic) and minim ...200717320991
coculture-inducible bacteriocin activity of lactobacillus plantarum strain j23 isolated from grape must.detection and characterization of bacteriocin production by lactobacillus plantarum strain j23, recovered from a grape must sample in spain, have been carried out. bacteriocin activity was degraded by proteolytic enzymes (trypsin, alfa-chymotrypsin, papaine, protease, proteinase k and acid proteases), and it was stable at high temperatures (121 degrees c, 20min), in a wide range of ph (1-12), and after treatment with organic solvents. l. plantarum j23 showed antimicrobial activity against oenoco ...200717367681
characterization of two bacteriocins produced by pediococcus acidilactici isolated from "alheira", a fermented sausage traditionally produced in portugal.lactic acid bacteria were isolated from "alheira" sausages that have been sampled from different regions in portugal. the sausages were produced according to different recipes and with traditional starter cultures. two isolates (ha-6111-2 and ha-5692-3) from different sausages were identified as strains of pediococcus acidilactici. each strain produces a bacteriocin, designated as bacha-6111-2 and bacha-5692-3. both bacteriocins are produced at low levels after 18 h of growth in mrs broth (3200 ...200717368595
antimicrobial susceptibilities of lactobacillus, pediococcus and lactococcus human isolates and cultures intended for probiotic or nutritional use.to determine mics of 16 antimicrobials representing all major classes for 473 taxonomically well-characterized isolates of lactic acid bacteria (lab) encompassing the genera lactobacillus, pediococcus and lactococcus. to propose tentative epidemiological cut-off (ecoff) values for recognizing intrinsic and acquired antimicrobial resistances in numerically dominant species.200717369278
species diversity and relative abundance of vaginal lactic acid bacteria from women in uganda and korea.lactobacilli play an important role in maintaining vaginal health of women. the aim of this study was to compare the species richness and relative abundance of lactobacillus and other lactic acid bacteria in women of two geographically distant countries, uganda and korea.200717381754
quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets.to explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (agps) on a vegetable-based diet.200717381758
effects of chinese wolfberry (lycium chinense p. mill.) leaf hydrolysates on the growth of pediococcus acidilactici.growth stimulating effects of lych leaf hydrolysates on pediococcus acidilactici imt101 cells were observed when mrs broth was supplemented with 20% (v/v) h1+h2, the mixture of hydrolysates prepared by a traditional tea-making process. cells grown on mrs containing h1+h2 showed a shortened lag phase while yielding a cell concentration (x(s)) significantly higher than other conditions investigated entering stationary phase. the maximal specific growth rate (mu(max)) was also the highest among all ...200817383176
simultaneous microchip enzymatic measurements of blood lactate and glucose.a miniaturized capillary electrophoretic (ce) microchip device for the simultaneous measurements of lactate and glucose is described. the new microchip bioassay protocol integrates an electrophoretic separation of lactate and glucose, post-column enzymatic reactions of these metabolites with their respective oxidase enzymes, and an amperometric (anodic) detection of enzymatically-liberated hydrogen peroxide at a gold-coated thick-film carbon detector. factors influencing the response have been e ...200717386641
lactobacillus composti sp. nov., a lactic acid bacterium isolated from a compost of distilled shochu residue.two strains of lactic acid bacteria, strains nric 0689(t) and nric 0690, were isolated from a compost of distilled shochu residue in japan. the isolates showed quite low sequence similarity to known species of lactic acid bacteria on the basis of 16s rrna gene sequence; the highest sequence similarities to nric 0689(t) were shown by the type strains of lactobacillus satsumensis, l. plantarum, l. hilgardii, l. buchneri and l. parabuchneri (92.9, 92.9, 92.8, 92.6 and 92.5 %, respectively). the iso ...200717392222
peoc01: a plasmid from pediococcus acidilactici which encodes an identical streptomycin resistance (aade) gene to that found in campylobacter jejuni.the complete nucleotide sequence of peoc01, a plasmid (11,661 bp) from pediococcus acidilactici ncimb 6990 encoding resistance to clindamycin, erythromycin, and streptomycin was determined. the plasmid, which also replicates in lactococcus and lactobacillus species contains 16 putative open reading frames (orfs), including regions annotated to encode replication, plasmid maintenance and multidrug resistance functions. based on an analysis the plasmid replicates via a theta replicating mechanism ...200717395262
identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations.presumptive lactic acid bacterial cocci were found in six sourdoughs (out of 20) from the abruzzo region (central italy) and subjected to phenotypic and genotypic characterization. a total of 21 isolates, recognized as seven strains by randomly amplified polymorphic dna (rapd)-polymerase chain reaction (pcr) typing, were identified by a polyphasic approach, consisting of 16s rrna gene sequencing, multiplex pcr assays and physiological features, as enterococcus faecium and pediococcus pentosaceus ...200717418310
pretreatment with pro- and synbiotics reduces peritonitis-induced acute lung injury in rats.to study whether enteral pretreatment with a synbiotic composition of lactic acid bacteria and bioactive fibers can reduce peritonitis-induced lung neutrophil infiltration and tissue injury in rats.200717426542
identification of lactic acid bacteria from fermented tea leaves (miang) in thailand and proposals of lactobacillus thailandensis sp. nov., lactobacillus camelliae sp. nov., and pediococcus siamensis sp. nov.eighteen rod-shaped homofermentatives, six heterofermentatives, and a coccal homofermentative lactic acid bacteria were isolated from fermented tea leaves (miang) produced in the northern part of thailand. the isolates were placed in a monophyletic cluster consisting of lactobacillus and pediococcus species. they were divided into seven groups by phenotypic and chemotaxonomic characteristics, dna-dna similarity, and 16s rrna gene sequences. groups i to vi belonged to lactobacillus and group vii ...200717429157
effects of pediococcus- and saccharomyces-based probiotic (mitomax) on coccidiosis in broiler chickens.coccidiosis is the major parasitic disease of poultry. in this study, the role of the commercial probiotic mitomax which contains pediococcus acidilactici and saccharomyces boulardii was evaluated by measuring body weight gain, fecal oocyst shedding, and serum antibody responses as an alternative control method of prophylactic drug against coccidiosis. day-old broiler chicks were fed regular or probiotic diets supplemented with mitomax at 0.01%, 0.1%, or 1.0% of diet, and challenged 2 weeks late ...200717434586
pcr monitoring of lactobacillus and bifidobacterium dynamics in fermentations by piglet intestinal microbiota.a new group-specific primer (lact71r), targeting the 16s-23s rdna intergenic spacer region of lactobacillus, was tested in its specificity to amplify rdna of lactobacilli from piglet intestinal origin by polymerase chain reaction (pcr). lact71r and lab0677f, a lactobacillus group-specific primer targeting the 16s rdna, generated a common amplicon by pcr with dna from lactobacillus and pediococcus reference strains, but not from weissella strains. sequence analysis of clones obtained by pcr ampli ...200717440917
sequence analysis of the mobilizable lactococcal plasmid pgdh442 encoding glutamate dehydrogenase activity.a novel plasmid named pgdh442 had previously been isolated from a plant lactococcus lactis strain. this plasmid encodes two interesting properties with applications in the dairy industry: a glutamate dehydrogenase activity that stimulates amino acid conversion to aroma compounds, and cadmium/zinc resistance that can be used as a selectable marker. moreover, this plasmid can be transferred naturally to other strains, but appears to be incompatible with certain other lactococcal plasmids. during t ...200717464081
development of thermal surrogate microorganisms in ground beef for in-plant critical control point validation studies.in search of a suitable surrogate microorganism for in-plant critical control point validation, we compared the rates of thermal inactivation of three bacteria, enterococcus faecium b2354, pediococcus parvulus hp, and pediococcus acidilactici lp, to those of listeria monocytogenes and salmonella. ground beef samples containing 4 and 12% fat were inoculated with e. faecium, l. monocytogenes, and salmonella senftenberg 775w and heated at 58, 62, 65, or 68 degrees c. the decimal reduction times (d- ...200717477266
high resolution crystal structure of pedb: a structural basis for the classification of pediocin-like immunity proteins.pediocin-like bacteriocins, ribosomally-synthesized antimicrobial peptides, are generally coexpressed with cognate immunity proteins in order to protect the bacteriocin-producer from its own bacteriocin. as a step for understanding the mode of action of immunity proteins, we determined the crystal structure of pedb, a pediocin-like immunity protein conferring immunity to pediocin pp-1.200717537233
modelling the effect of high pressure on the inactivation kinetics of a pressure-resistant strain of pediococcus damnosus in phosphate buffer and gilt-head seabream (sparus aurata).the aim of this research was to: (i) determine the inactivation pattern of a pressure-resistant strain of pediococcus damnosus by high hydrostatic pressure in phosphate buffer (ph 6.7) and gilt-head seabream using the linear, biphasic and weibull models; and (ii) validate the applicability of the weibull model to predict survival curves at other experimental pressure levels.200717578414
development of innovative pediocin pa-1 by dna shuffling among class iia bacteriocins.pediocin pa-1 is a member of the class iia bacteriocins, which show antimicrobial effects against lactic acid bacteria. to develop an improved version of pediocin pa-1, reciprocal chimeras between pediocin pa-1 and enterocin a, another class iia bacteriocin, were constructed. chimera ep, which consisted of the c-terminal half of pediocin pa-1 fused to the n-terminal half of enterocin a, showed increased activity against a strain of leuconostoc lactis isolated from a sour-spoiled dairy product. t ...200717601819
lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin.to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development.200717650194
influence of geographical origin and flour type on diversity of lactic acid bacteria in traditional belgian sourdoughs.a culture-based approach was used to investigate the diversity of lactic acid bacteria (lab) in belgian traditional sourdoughs and to assess the influence of flour type, bakery environment, geographical origin, and technological characteristics on the taxonomic composition of these lab communities. for this purpose, a total of 714 lab from 21 sourdoughs sampled at 11 artisan bakeries throughout belgium were subjected to a polyphasic identification approach. the microbial composition of the tradi ...200717675431
a taxonomic survey of lactic acid bacteria isolated from wheat (triticum durum) kernels and non-conventional flours.in order to explore the correspondence between raw material- and mature sourdough-lactic acid bacterial (lab) communities, 59 italian wheat (triticum durum) grain samples, one bran and six non-conventional flour samples were analyzed through a culture-dependent approach. the highest cell count by an agar medium specific for lab was 2.16 log cfu/g. from about 2300 presumptive lab (gram-positive and catalase-negative) colonies collected, a total of 356 isolates were subjected to identification by ...200717683888
ecological removal of recalcitrant phenolic compounds of treated olive mill wastewater by pediococcus pentosaceus.treatment of olive mill wastewater by different biological process led to an important decrease of organic compounds but the black coloration due to the recalcitrant phenolic compounds persists. experimental design using fractional factorial plan showed that, among the seven studied factors, yeast extract, dilution and glucose exhibited a positive effect on pediococcus pentosaceus growth and treated olive mill wastewater (tomw) decolourisation. optimization of influent factors showed that 2.5 an ...200817686628
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