| isolation and characterization of sheep pepsin. | sheep pepsin was isolated (approx. 120-fold purification) from aqueous abomasal homogenates by (1) ph fractionation, (2) chromatography on sepharose 4b-poly-l-lysine columns and (3) gel filtration on sephadex g-100. the enzyme had mol.wt. approx. 34000, n-terminal valine and c-terminal alanine. the amino acid composition of sheep pepsin was generally similar to that of pig and ox pepsins, with a very low content of basic residues and a high content of acidic and hydroxy-amino acids. the ph optim ... | 1977 | 15546 |
| the transport of l-leucine into the brain of the rat in vivo: saturable and non-saturable components of influx. | | 1977 | 16270 |
| purification and properties of the nad(p)h:nitrate reductase of the yeast rhodotorula glutinis. | the assimilatory nitrate reductase from the yeast rhodotorula glutinus has been purified 740-fold, its different catalytic activities have been characterized and some inhibitors studied. the purified enzyme (150 units per mg protein) contains a cytochrome of the b-557 type. an s20,w of 7.9 s was found by the use of sucrose density gradient centrifugation, and a stokes radius of 7.05 nm was determined by gel filtration. from these values, a molecular weight of 230 000 was estimated for the native ... | 1977 | 18182 |
| narcotic cueing properties of intraventricularly administered sufentanil, fentanyl, morphine and met-enkephalin. | the narcotic cueing activity of sufentanil, fentanyl, morphine and met-enkephalin was studied upon their injection into the lateral brain ventricle of the rat. comparative studies on the analgesic activity of the three narcotics support a close correlation between the narcotic cueing and the analgesic activity of narcotic drugs. | 1978 | 22444 |
| studies on the structure-activity relationship of adrenergic beta-mimetic benzylamine derivatives. iv.1) aryl-substituted 1-aminotetralins and 1-aminoindans. | | 1978 | 34486 |
| incomplete cardiac vagal blockade with atropine in the anesthetized dog. | in dogs in neurolept-anesthesia the successive administration of alpha- and beta-adrenergic blocking agents and atropine, which should cause the functional equivalent of surgical denervation of the heart, always results in a marked tachycardia. the same is observed in conscious dogs, but not during methoxyflurane anesthesia. bilateral vagotomy and administration of hexamethonium abolish the tachycardia. these observations demonstrate the presence of a vagally mediated chronotropic effect which b ... | 1979 | 42039 |
| renal cytotoxic effects of bence-jones protein. | | 1977 | 64688 |
| serum and cellular biologic tumor markers in patients with urologic cancer. | during the past several years the development of radioimmunoassay and immunocytochemical techniques to detect small amounts of marker in the sera and cancer cells of cancer patients has made a significant impact on the diagnosis and management of certain cancers. among these markers alpha-fetoprotein, human chorionic gonadotropin, and pregnancy specific beta-1 glycoprotein have been useful in the staging, detection of recurrence, prognosis, and management of testicular cancer. in this article th ... | 1979 | 93569 |
| the cortico-cortical connections to area 5 of the parietal lobe from the primary somatic sensory cortex of the monkey. | | 1978 | 24219 |
| synthetic antigens iii. antigen-binding cells to interpolymer of styrene and maleic acid psm and sheep red blood cells in mice immunized with psm. | studies on the immune response in mice to a new synthetic antigen interpolymer of styrene and maleic acid (psm) are described. the immune response was measured by determination of antigen binding cells by means of rosette test with psm-coated srbc, an unexpected effect was observed--besides rosette-forming cells for psm, rosette-forming cells and agglutinating antibodies for sheep red cells were found. the results presented in this paper suggest that psm is acting both as an immunogen and a poly ... | 1979 | 94989 |
| n-acetylglucosamine-6-sulfate sulfatase in man: deficiency of the enzyme in a new mucopolysaccharidosis. | | 1978 | 99718 |
| concentrations of oestradiol-17beta and progesterone in the peripheral plasma of the cynomolgus monkey (macaca fascicularis) in relation to the length of the menstrual cycle and its component phases. | ninety-three female cynomolgus monkeys were monitored throughout 647 menstrual cycles; 93.2% of these cycles were 22--37 days long and were normally distributed, with a mean of 29.2 days. menstrual bleeding generally lasted for 3--5 days and was not related to the length of the cycle. the levels of progesterone and oestradiol-17beta in the plasma were measured during the menstrual cycle in 30 monkeys. the concentration of oestradiol-17beta reached a mid-cycle peak on day 11 or 12 of the cycle. t ... | 1978 | 101640 |
| alpha-adrenergic blocking and beta-adrenergic agents --antidotes for cyanide toxicity--. | | 1977 | 21320 |
| isolation of an influenza virus subtype hav4navl from a budgerigar. | | 1979 | 108521 |
| separation of dna-dependent dna polymerase activities in micrococcus radiodurans. | dna polymerase activities in micrococcus radiodurans were separated into two fractions after purification more than 2000 fold. they differ in ph optimum and residual activities in the absence of a full deoxyribonucleoside triphosphates complement. nad partly inhibited one of the activities. both activities were eluted as a single peak on gel filtration and sedimented at the same rate on glycerol gradient centrifugation. molecular weight 140000 was calculated from stokes radius and sedimentation ... | 1977 | 14686 |
| individual variability and correlation of the in vitro tests in tolerant rats. | cytotoxicity of lymphocytes, mixed lymphocyte cultures, stimulation with pha, suppressive activity of cells in vitro and efficacy of the adoptive transfer of cells in vivo were simultaneously investigated in rats bearing tolerated skin allografts. cells from animals which did not react in mlc were simultaneously hyporeactive when stimulated with pha. on the other hand, cytotoxicity of lymphocytes did not correlate with their ability to respond to specific antigens in mlc. none of the in vitro te ... | 1978 | 147185 |
| glycolysis mutants in saccharomyces cerevisiae. | mutants have been isolated in s. cerevisiae with the phenotype of growth on pyruvate but not on glucose, or growth on rich medium with pyruvate but inhibition by glucose. screening of mutagenized cultures was either without an enrichment step, or after enrichment using the antibiotic netropsin (young et al. 1976) or inositol starvation (henry, donahue and culbertson 1975). one class of mutants lacked pyruvate kinase (pyk), another class had all the enzymes of glycolysis, and one mutant lacked ph ... | 1978 | 147195 |
| neovasculogenesis. triggering factors and possible mechanisms. | the possible regulating mechanism(s) of neovascularization are discussed. experimental data supporting the view that ocular neovascularization might be triggered by a fundamental metabolic mechanism are presented. based on his own data and scattered evidence from the literature, the author suggests a model for a possible regulating mechanism of the neovascular process in which the prostaglandins fulfill the role of neovascular mediating substances. preliminary results demonstrating that indometh ... | 1979 | 94468 |
| thyroid function in preterm infants with respiratory distress syndrome. | thyroid function was evaluated in premature infants 30 to 35 weeks ega with severe rds and was compared to that of healthy control subjects of similar ega. the infants with rds had significantly lower serum levels of t3, t4, and ft4i during the first 60, 90, and 30 days, respectively, and had elevated t3ur during the first 10 days. the mean (+/- sd) postdelivery tsh peak was lower in the rds group (32.8 +/- 9.6 muu/ml) than in the control group (60.9 +/- 21.8 muu/ml; p less than 0.005). after tr ... | 1979 | 107304 |
| [use of intraspecific differentiation of sh. sonnei for the purpose of studying patterns in the epidemic process of dysentery]. | the use of complex typing of sh. sonnei by the colicinogenic and enzymatic properties in epidemiological analysis of dysentery caused by sh. sonnei, apart from establishment of epidemiological connections in the foci, offered a possibility of introducing significant corrections in the determination of the sizes and duration of existence of the epidemic foci, as well as in detection of the sources and factors of transmission of the infection established in epidemiological examination. typing of s ... | 1978 | 154276 |
| oncogenic transformation of c3h/10t1/2 clone 8 mouse embryo cells by halogenated pyrimidine nucleosides. | oncogenic transformation has been induced in vitro in the c3h/10t1/2 clone 8 line of mouse cells by exposure to 5-fluoro-2'-deoxyuridine (fudr) or 5-fluorouracil. this transformation is both dose and time dependent and can be markedly decreased by simultaneous exposure of the cells to thymidine. the transformation induced by 5-fluorouracil is probably due to its intracellular conversion to fudr or its monophosphate. transformation by fudr was found to be cell cycle dependent with maximum sensiti ... | 1976 | 129280 |
| atp-ase activity of purified human normal t- and b-lymphocytes. | in 15 healthy individuals the atp-ase activity of circulating b-lymphocytes was found to be significantly higher than of t-lymphocytes. two different methods were used for the purification of b- and t-lymphocytes and the result was independent of which one of these methods was used. the demonstration of different atp-ase activities of unstimulated b- and t-lymphocytes corresponds well with previously published histochemical findings in lymphatic tissues examined for atp-ase activity. it is concl ... | 1978 | 148099 |
| [human cytogenetic study methods--value and use. ii. banding methods in chromosome diagnosis]. | | 1979 | 159568 |
| ultrastructural features of the neuromuscular spindle in the dystrophic mouse. | | 1979 | 159888 |
| [use of the concanavalin-peroxidase-dab system for identification of human chromosomes]. | the effect of concanavalin a on the prometaphase chromosomes was investigated, using a staining reaction based on the peroxydase-diaminobenzidin-h2o2 system. after incubation with concanavalina a, the chromosomes telomeres as well as the centromeres and satellites of the acrocentric chromosomes were strongly stained. sometimes the chromatids appeared to be coiled. in other respect, it must be noted that peroxydase alone can stain the chromatids, which probably means that this compounds is able t ... | 1979 | 160811 |
| luteinized unruptured follicle syndrome: a subtle cause of infertility. | a clinical description of luteinized unruptured follicles is presented. this abnormality in ovulation is characterized by normal endocrinologic presumptive signs of ovulation: biphasic basal body temperature curves, secretory endometrium, and laboratory evidence of progesterone production by elevated urinary pregnanediol or plasma progesterone levels. in a group of 102 such infertile women, laparoscopy performed 3 to 5 days after apparent ovulation revealed the absence of a corpus hemorrhagicum ... | 1978 | 147778 |
| morphine abstinence is associated with increased brain cyclic amp. | | 1975 | 165429 |
| the fibrinolysin system and its relationship to disease in the newborn. | the fibrinolysin system is incomplete in newborn infants. lack of serum plasminogen in premature newborn has an important role in the pathophysiology of the respiratory distress syndrome since alveolar fibrin deposits cannot be eliminated. urokinase activated human plasmin has increased the survival rate of infants with respiratory distress syndrome. plasminogen given i.v. at birth has reduced the incidence and the severity of respiratory distress syndrome, in a randomized double-blind study of ... | 1979 | 161694 |
| [evaluation of the outer envelope antigens of leptospira in complement fixation and hemagglutination tests for leptospirosis]. | | 1979 | 158370 |
| [integration and excision of transducing lambda phages carrying rna-polymerase genes]. | | 1979 | 159205 |
| use of cryopreserved virus-infected target cells in a lymphocytotoxicity 51cr release microassay for cell-mediated immunity to cytomegalovirus. | a new 51cr-release microassay is described for the measurement of cell-mediated immunity to cytomegalovirus, using cryopreserved target cells acutely infected with cytomegalovirus. | 1976 | 177371 |
| in vitro translation of encephalomyocarditis viral rna: synthesis of capsid precursor-like polypeptides. | | 1976 | 178097 |
| characterization of the parathyrin receptor in renal plasma membranes by labelled hormone and labelled antibody binding techniques. | the parathyrin receptor in renal cortex has been investigated by studying the binding of 125i-labelled parathyrin, or of unlabelled parathyrin detected with 125i-labelled antibodies, to a partially purified plasma membrane fraction. the kinetics of hormone uptake demonstrated a biphasic response in both systems at 22 degrees c but this phenomenon was not detectable at 37 degrees c. specific displacement of lactoperoxidase labelled 125i-labelled parathyrin occurred with 8 ng unlabelled bovine par ... | 1976 | 178366 |
| altered surface topology and membrane functions of rat thymocytes eluted from nylon wool columns. | (1) following incubation of thymocytes with nylon wool at 37 degrees c, the eluted cells showed an increase in the number of microvilli per cell and a concominant elongation of the microvilli (0.22 mum versus 1.15 mum. (2) cyclic adenosine monophosphate (cylic amp) levels were lowered by 30-50% in nylon wool-treated thymocytes. (3) nylon wool-treated cells showed an impaired na+-dependent amino acid transport system (2-aminoisobutyrate) whereas the na+-independent amino acid transport system (1- ... | 1976 | 177058 |
| complementation of ts mutants by a herpes simplex virus ts-transformed cell line. | | 1976 | 179018 |
| neural properties of cultured human endocrine tumor cells of proposed neural crest origin. | cells from human endocrine tumors of proposed neural crest origin--five pheochromocytomas, two medullary carcinomas of the thyroid, and two bronchial carcinoids--were grown in monolayer culture. cells from all nine tumors, including epithelial forms of medullary carcinoma of the thyroid and bronchial carcinoid cells, and epithelial and neuron-like pheochromocytoma cells demonstrated all-or-nothing, short-duration action potentials. | 1976 | 179139 |
| [study of the indirect complement fixation test for use in the differentiation of foot-and-mouth disease viruses and the determination of immunity]. | studies were carried out to establish the optimal conditions for the indirect complement-fixation test (icft) and explore the possibility to use the test in differentiating the foot-and-mouth disease (fmd) viruses and checking the immunity obtained in survivals or in animals that had been vaccinated against fmd. it was demonstrated that the proper use of icft necessitates in turn the use of a minimal amount of antigen, which in the presence of 1-2 e homologous hyperimmune serum completely binds ... | 1975 | 179191 |
| avian myeloblastosis virus-induced lymphosarcoma producing erythroblastic leucosis in chicks. | acute myeloblastosis and several forms of tumor, including one case of lymphosarcoma occurred when avian myeloblastosis virus (bai-a strain) was inoculated into newly hatched chicks (spf). the homogenate of lymphosarcoma inoculated intraperitoneally into other newly hatched chicks induced a high incidence of erythroblastic leucosis. electron microscopy did not reveal the presence of c-type virus particles in the tumor tissue. the relationship between avian myeloblastosis virus, lymphosarcoma and ... | 1975 | 179285 |
| the effect of antilipolytic agents on cyclic amp, free fatty acid and total catecholamine concentrations in plasma. | plasma concentrations of adenosine 3':5' cyclic monophosphate (cyclic amp) and free fatty acids have been measured in 15 patients with acute myocardial infarction and in 6 dogs given infusions of isoprenaline. plasma total catecholamines were also estimated in the patients. inhibition of adipose tissue lipolysis with a nicotinic acid analogue did not decrease plasma cyclic amp concentrations, either in the patients or when elevated in the dogs, thus suggesting that this tissue is not a major sou ... | 1976 | 179286 |
| [sensitivity of various races of the tularemia microbe to erythromycin, oleandomycin and polymyxin]. | sensitivity of 66 strains of the tularemia microbe to erythromycin, oleandomycin and polymyxin was studied. it was shown that all the strains were resistant to polymyxin. the strains of the non-artic and central asiatic races and 64 per cent of the strains of the holarctic race were resistant to erythromycin and oleandomycin. | 1976 | 179462 |
| [study of c1. perfringens resistance to lincomycin]. | a possibility of developing resistant forms of c1. perfringens during treatment of experimental anaerobic (gaseous) infection with lincomycin was studied. it was shown that treatment of the animals for 7 days resulted in an increase in the resistance by 33-41 times. it was noted that strains with decreased sensitivity to lincomycins had changed morphology and biochemical activity (decreased lecitinase activity, changed biochemical properties), decreased virulence and pathogenicity for animals. s ... | 1976 | 179463 |
| primary intracranial burkitt's lymphoma in an infant. | a case of intracranial burkitt's lymphoma is reported in a child whose symptoms began at 3 months of age with a definite histologic diagnosis established at 18 months. serologic studies demonstrated high antibody titers to epstein-barr virus (ebv) in the patient and in four out of five members of the immediate family. the patient also demonstrated immunity to antigens derived from african burkitt's lymphoma cell lines. the autopsy findings strongly support the case for the primary intracranial o ... | 1976 | 177169 |
| [protective effect of complexogenic drugs (cysteamine, cysteine) on some enzyme activities of the kidney of rats treated with chlormerodrin-hg197. histochemical studies]. | | 1975 | 177031 |
| [histology, fine structure and differentiation of experimental wilms tumors (author's transl)]. | histologic studies indicate that the cycasin-induced wilms' tumor in the rat is equal to human nephroblastoma in appearance. therefore, it may represent an interesting system for experimental oncology. in electron microscopy, the spindle cells of the sarcoma region mostly represent tubular cells. besides, the author's results showed that preexisting mesenchyma cells and blood vessels also form part of the tumor. electron microscopy studies have shown that cycasin feeding will lead to early cellu ... | 1978 | 204826 |
| [distribution and activity of pyridoxal kinase in human brain during ontogenesis]. | the activity of pyridoxal kinase was sharply increased in whole brain tissue of human, embryos and fetuses within 6-11 weeks of development. in brain stem the maximal values of the enzyme activity was observed at early stages of prenatal development of fetuses. the activity of pyridoxal kinase was increased in cerebral cortex and in the limbic system up to complete maturation of fetuses. it correlated with the fetus age within 14-40 weeks of development as calculated per 1 g of tissue wight or 1 ... | 1976 | 179608 |
| intratumor chemoimmunotherapy with mitomycin c and components from mycobacteria in regression of line 10 tumors in guinea pigs. | intratumor chemotherapy with the use of mitomycin c and/or immunotherapy caused regression of line 10 carcinomas in strain 2 guinea pigs and resulted in development of tumor-specific immunity. the immunotherapeutic preparation consisted of oil-in-water emulsions containing mycobacterium cell walls or residues of cell walls termed cell wall skeletons. the latter preparations were combined with trehalose dimycolate, which was isolated by microparticulate chromatography from whole cells of mycobact ... | 1978 | 205361 |
| intracellular distribution and sedimentation properties of virus-specific rna in two clones of bhk cells transformed by polyoma virus. | the virus-specific rna in two independently derived clones of polyoma virus-transformed hamster cells was studied by hybridizing labeled rna, with excess purified polyoma dna, immoblized on filters. in one clone (pybhk1), less than 25% of the total labeled virus-specific rna was found in the cytoplasm, irrespective of the labeling time. in the other clone (pybhk2), it was estimated that 39% of the total virus-specific rna was present inthe cytoplasm after labeling for 3 h. both the proportion of ... | 1976 | 178888 |
| effect of treatment with exogenous interferon, polyriboinosinic-polyribocytidylic acid, or polyriboinosinic-polyribocytidylic acid-poly-l-lysine complex on herpesvirus hominis infections in mice. | the effect of treatment with exogenous interferon was compared with those of two interferon inducers, polyriboinosinic-polyribocytidylic acid [poly(i) . poly(c)] and poly(i) . poly(c)-poly-l-lysine complex [poly(iclc)], in three model herpesvirus hominis type 2 infections of mice. after intraperitoneal inoculation of h. hominis type 2, all drugs significantly protected animals against death and increased the mean day of death when administered as late as 48 hr after viral inoculation. with intra ... | 1978 | 206632 |
| the effects of nerve growth factor and its antiserum on synapses in the superior cervical ganglion of the guinea-pig. | 1. the effects of nerve growth factor (ngf) and its antiserum on synapses in the superior cervical ganglion of the guinea-pig have been examined by intracellular recording and electron microscopy. 2. exogenous ngf, supplied locally from a silicone rubber pellet implanted near ganglia for 4-7 days, had little effect on either the function or the number of ganglionic synapses. 3. however, the depression of synaptic transmission and loss of synaptic contacts on ganglion cells which follow post-gang ... | 1978 | 206691 |
| hepatitis a. | | 1977 | 205765 |
| respiratory disease in horses. | | 1977 | 196170 |
| human beta-endorphin: the real opium of the people? | | 1978 | 209845 |
| comparison of interferon action in interferon resistant and sensitive l1210 cells. | translation inhibition, leu-trna aminoacylation and double-stranded rna and atp dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse l 1210 r and l 1210 s cells. no differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67k dalton phosphoprotein fraction in interferon-treated l 1210 s cell-free extracts. in non-responding cell-free lysates, the lack of sti ... | 1978 | 212514 |
| the binding isotherms for the interaction of 5-doxyl stearic acid with bovine and human albumin. | binding isotherms for the interaction of 5-doxyl stearic acid with bovine and human albumin are reported. the critical micelle concentration (cmc) and the limiting solubility of 5-doxyl stearic acid were determined using the electron spin resonance (esr)-spin label method. the cmc and the limiting solubility of this spin-label stearic acid in saline-phosphate buffer are 3.5 x 10(-5) m and 2 x 10(-4) m, respectively. we found no esr line width evidence for pre-association of the spin-label steara ... | 1978 | 213518 |
| necrotizing pancreatitis due to simian adenovirus type 31 in a rhesus monkey. | simian adenovirus type 31 was isolated from pancreatic tissue of an 8-year-old male rhesus monkey that died as the result of acute necrotizing pancreatitis. histologically, the pancreas showed wide-spread necrosis, extensive infiltration of polymorphonuclear leukocytes, and nuclear inclusions in pancreatic acinar cells. large numbers of adenovirus particles were demonstrable in the acinar cells by electron microscopy. this is the second case of adenovirus-associated pancreatitis we have found in ... | 1978 | 205187 |
| secretory granules of the duodenal enterochromaffin cells of the cat. | | 1979 | 222959 |
| isolation and characterization of two threonine-poor apolipoproteins of human plasma high density lipoproteins. | | 1978 | 208597 |
| effects of fildes enrichment on antibiotic susceptibility testing of haemophilus influenzae in the autobac. | the results of this investigation showed that the autobac could be used to determine the susceptibility of haemophilus influenzae to ampicillin, chloramphenicol, and tetracycline when eugonic broth was supplemented with 1.5% fildes enrichment. an excellent correlation was obtained between the susceptibilities as determined by the agar dilution minimal inhibitory concentration method and the autobac. for ampicillin all h. influenzae isolates with minimal inhibitory concentrations of </=1 mug/ml h ... | 1979 | 314272 |
| site-specific recombination leading to the integration of phages p1 and p7. | | 1979 | 385221 |
| specific inhibition of macrophage migration inhibition factor by fucosylated glycolipid rm. | the effects of glycolipids on the interaction of the mif (migration inhibition factor) with rat macrophages were examined using a migration inhibition assay system. mif activity was specifically blocked by fucosylated glycolipid rm [gal alpha 1-3gal(2-1 alpha fuc) beta 1-3galnac beta 1-3gal beta 1-4glc beta 1-1ceramide, (1978) j. biochem. 83, 85-90], but not by cytolipin r, hematoside, or blood group b active glycolipid [gal alpha 1-3gal(2-1 alpha fuc) beta 1-4glcnac beta 1-3gal beta 1-4glc beta ... | 1979 | 389925 |
| effect of interferon on transcription and translation of vesicular stomatitis virus in human and simian cell cultures. | the effect of interferons on vesicular stomatitis virus (vsv) primary transcription, amplified rna synthesis [i.e. the sum of primary transcription rna replication (leading to [ - ] rna) and secondary transcription (leading to [ + ] rna) and virus protein synthesis were studied. in a human cell line, both human and simian interferons inhibited the initiation of primary transcription and amplified rna synthesis. in contrast, in a simian cell line tested similarly, the initiation of these activiti ... | 1978 | 214523 |
| synthesis and quantitative structure-activity relationships of antibacterial 1-(substituted benzhydryl)-4-(5-nitro-2-furfurylideneamino) piperazines. | 1-benzhydryl -4- (5-nitro-2-furfurylideneamino) piperazine and 11 substituted analogs were prepared and examined for in vitro antimicrobial activity. the compounds were active against bacillus cereus 7, bacillus megaterium 122, bacillus subtilis 104, clostridium perfringens 13, and the tetracycline-resistant clostridium perfringens 37. regression analyses on the antibacterial activity data based on the hansch approach, using pi, pi2, and sigma parameters, yielded several statistically significan ... | 1978 | 207855 |
| lung compliance and lung morphology following artificial ventilation in the premature and full-term rabbit neonate. | rabbit neonates delivered on day 27--30 of gestation were subjected to intermittent positive pressure ventilation (ippv) for 60 min. tidal volume was standardized to 10 ml/kg, and the insufflation pressure required to maintain this tidal volume was recorded. the quasistatic compliance of the lung-thorax system, corrected for variations in body weight, was significantly lower in animals delivered on day 27--28 than in those delivered on day 29--30. immature animals with low lung-thorax complianc ... | 1979 | 394314 |
| neurotensin: antinocisponsive action in rodents. | | 1979 | 421735 |
| chromosome assignments of genes in man using mouse-human somatic cell hybrids: association of diphtheria toxin sensitivity with chromosome 5. | | 1975 | 1192802 |
| endophthalmitis associated with haemophilus influenzae type b bacteremia and meningitis. | the unusual occurrence of endophthalmitis associated with haemophilus influenzae type b bacteremia and meningitis was confirmed in three young children during a five-year period. in contrast to bacterial endophthalmitis attributed to other microorganisms, these three infections resolved completely within a few days with conventional antimicrobial therapy. endophthalmitis is but one of the apparently increasing number of unsuual complications that may be observed as a result of h influenzae type ... | 1979 | 312601 |
| [bile and stress ulcer in the rat (author's transl)]. | the influence of bile on the ulcer formation under "restraint stress" condition was studied in rats with direct choledocho-gastrostomia resp. bile duct ligation. after 9 hrs. of immobilisation in plaster cuffs the animals with unrestricted bile flow via the choledochosstress gastrostomia showed as many ulcers as the sham-operated controls. there was no difference in the ulcer localisation, the region of the bile duct anastomosis was without any ulcer. after bile duct ligation the ulcer frequency ... | 1975 | 1214546 |
| impairment of proteoglycan-collagen interaction in early experimental osteoarthrosis. | | 1975 | 1221943 |
| [autognostic human activities]. | | 1975 | 1137908 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| [inhibiton of isocitrate dehydrogenase and isocitrate lyase from acinetobacter calcoaceticus by acids of the citrate and glyoxylate cycle]. | acinetobacter calcoaceticus contains two forms of nadp+-dependent isocitrate dehydrogenases differing, among others, by their molecular weights and regulatory properties. the regulation of the high-molecular form of isocitrate dehydrogenase and of isocitrate lyase by organic acids, either belonging or related to the citrate and glyoxalate cycle, is investigated. while alpha-ketoglutarate and oxalacetate competitively inhibit the isocitrate dehydrogenase against ds-isocitrate, glyoxylate and pyru ... | 1975 | 1199592 |
| gastric uptake of gallium-67 in aids. | we describe gastric localization of 67ga in 13 patients with acquired immunodeficiency syndrome (aids) among 148 referred primarily to rule out pneumocystis carinii pneumonia (pcp). endoscopic biopsies in five of the patients indicated cytomegalovirus (cmv) infection in one, gastritis in two, and normal tissue in two. other associated, but nongastric, infections in these 13 patients included esophageal candidiasis, pcp, mycobacterium avium-intracellulare (mai) complex, coccidioidomycosis, toxopl ... | 1992 | 1314894 |
| leukotriene b4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa. | human polymorphonuclear leukocytes (pmnl) metabolize the potent chemotaxin leukotriene b4 (ltb4) by omega-oxidation to 20-hydroxyl-ltb4 and 20-carboxy-ltb4. the ability of unstimulated human pmnl to metabolize exogenous ltb4 was found to be inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa, in a dose-dependent manner. 1-hydroxyphenazine (1-ohp), a metabolite of pyocyanin, was not inhibitory under identical conditions. the initial enzymic step in the conversion of ... | 1992 | 1316878 |
| alpha 2-adrenoceptor density in forebrain areas of zucker rats: no effect of genetic obesity or antiglucocorticoid treatment. | the density of alpha 2-adrenoceptors was determined in different forebrain regions of lean and obese zucker rats by para-[125i]iodoclonidine ([125i]pic) receptor autoradiography. no significant differences were observed between the zucker phenotypes in the density of alpha 2-adrenoceptors in any brain areas examined. four days treatment with the antiglucocorticoid mifepristone (ru 38486) did not affect [125i]pic-binding. it is concluded that the hyperphagia and abnormal corticosterone secretion ... | 1992 | 1322226 |
| characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis. | alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ... | 1992 | 1370473 |
| biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis. | the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ... | 1992 | 1370931 |
| mouse hypersensitivity pneumonitis: depletion of nk cells abrogates the spontaneous regression phase and leads to massive fibrosis. | the contribution of natural killer cells (nk) in the progression of mouse hypersensitivity pneumonitis induced by repeated intranasal instillations with the thermophilic actinomycete faeni rectivirgula was examined. these instillations determined a very large increase in the lung index (ca. twofold at 3 weeks), used as a measure of inflammation. in addition, this instillation was associated with a tenfold increase in the number of cells recovered in the bronchoalveolar lavage (bal) at 3 weeks an ... | 1992 | 1468409 |
| aneuploidy in mouse fetuses after paternal exposure to x rays. | | 1979 | 450138 |
| use of an enzyme-linked immunosorbent assay to monitor the control of staphylococcus aureus mastitis. | conventional culture methods were used to evaluate the ability of an elisa to identify staphylococcus aureus imi. the test was 96% accurate; sensitivity was 90%, and specificity was 97%. the test was used to screen preserved milk samples rapidly in 10 cooperator herds. prevalence of imi was greater than 10% in 6 herds at the first test. average prevalence of cows scoring +2 (suspect) and +3 (positive) was 12.6%. prevalence declined during the 12-mo study. incidence of new imi decreased from 7.9% ... | 1992 | 1560137 |
| structural and functional analyses of the repressor, rbsr, of the ribose operon of escherichia coli. | the dna sequence encoding the rbs repressor protein, rbsr, has been determined. amino acid sequence analyses of the product of an rbsr-lacz fusion and of affinity-purified rbsr demonstrate that translation begins at an unusual codon, ttg, and that the initial amino acid is removed during maturation of the protein. dna-binding assays indicate that rbsr binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site and that the affinity for the rbs operator is reduce ... | 1992 | 1304369 |
| antagonism of the motor effects of cgrp and of capsaicin on the guinea pig ileum by human cgrp8-37. | | 1992 | 1637118 |
| adrenalin activation of beta 2-adrenoceptors stimulates morphological changes in astrocytes (pituicytes) cultured from adult rat neurohypophyses. | neurohypophysial astrocytes, the pituicytes, are known to undergo morphological changes in vivo in response to stimuli that increase the demand for hormone secretion. similar changes have been induced by beta-adrenergic stimulation both in the isolated, but otherwise intact, neural lobe and in pituicytes cultured from adult rats. since the predominant beta-receptor subtype in the neural lobe is beta 2, we investigated the possibility that beta 2-receptor activation is mainly responsible for the ... | 1991 | 1682016 |
| antibacterial peptides designed as analogs or hybrids of cecropins and melittin. | eight new analogs of cecropin a, two new analogs of melittin and 30 hybrid peptides containing sequences from cecropins and melittin have been synthesized. the lengths of the peptides have varied from 37 residues (the length of cecropin a) to 18 residues. the peptides have been assayed for lysis of sheep red blood cells and for antibacterial activity against two gram negative and three gram positive bacteria. the best analogs of cecropin a maintained the anti-escherichia coli activity of the par ... | 1992 | 1483838 |
| [leiomyosarcoma of the right ventricle. report of a case and review of the literature]. | primary cardiac leiomyosarcoma is a very rare condition. only 15 cases have been reported in the literature. we report the case of a 27 year old man admitted to hospital for chest pain in march 1985. echocardiography showed a right ventricular tumour which was completely resected at surgery. the diagnosis of leiomyosarcoma was confirmed by histological examination. after 22 months follow-up, the patient was still alive despite pulmonary metastases. the diagnosis of cardiac tumour used to be made ... | 1991 | 1759901 |
| interactions of cadmium and selenium in rat plasma in vivo and in vitro. | 75se and 109cd tracers were used to study the binding of se and cd to plasma proteins at various seo32- doses and times upt to 24 h after the simultaneous subcutaneous administration of seo32- markedly increased both se and cd plasma levels over that in control animals. gel permeation chromatography of plasma indicated that at all times up to 24 h cd and se were bound in an atomic ratio of approx. 1 : 1 in 330 000 and 130 000 dalton fractions. from 4 to 24 h, cd and se appeared in the 420 000 da ... | 1976 | 1260013 |
| effect of spectrin on structure properties of lipid bilayers formed from mixtures of phospholipids. fluorescence and microcalorimetric studies. | effect of spectrin from human erythrocytes on structure properties of lipid bilayers formed from a mixture of phosphatidylethanolamine/phosphatidylserine (pe/ps) and/or phosphatidylethanolamine/phosphatidylcholine (pe/pc) was studied with the use of fluorescence and microcalorimetric methods. spectrin did not affect the order parameter of lipids in pe/ps vesicles. however, spectrin binding to liposomes did influence temperature, half-width and enthalpy of phase transitions in mixtures of dimyris ... | 1991 | 1769515 |
| comparison of in vitro biological properties and mouse toxicities of three thymidine analogs active against human immunodeficiency virus. | three analogs of thymidine, d4t [2',3'-didehydro-2',3'-dideoxythymidine; 1-(2,3-dideoxy-beta-d-glyceropent-2-enofuranosyl)thymine], fddt (3'-fluoro-3'-deoxythymidine), and azt (3'-azido-3'-deoxythymidine), were compared in biological tests designed to assess their potential utility as anti-human immunodeficiency virus (hiv) agents. the in vitro potencies of these compounds against hiv infection in cem cells were measured, with fddt and azt being more potent than d4t. the cytotoxicities of d4t, f ... | 1990 | 1693057 |
| processing of the glycoprotein of feline immunodeficiency virus: effect of inhibitors of glycosylation. | the processing and transport of the envelope glycoprotein complex of feline immunodeficiency virus (fiv) in the persistently infected crandell feline kidney (crfk) cell line were investigated. pulse-chase analyses revealed that the glycoprotein is synthesized as a precursor with an mr of 145,000 (gp145) and is quickly trimmed to a molecule with an mr of 130,000 (gp130). treatment of gp130 with endoglycosidase h (endo h) resulted in a protein with an mr of 75,000, indicating that nearly half the ... | 1991 | 1847441 |
| a monoclonal antibody against the sulfidopeptide leukotrienes ltc4, ltd4 and lte4. | a monoclonal antibody (1a-ldr1) against sulfidopeptide leukotrienes (lt) is described. the mab shows a nearly identical detection limit of about 0.04 ng for ltc4, ltd4, lte4 and naclte4 in standard fluid phase ria. steric modifications, however, diminish the sensitivity, as determined for the examples 5-epi-ltc4, 6-epi-ltc4, 5,6-epi-ltc4 and 11-trans-ltc4. no crossreactivity could be observed for ltb4. crossreactions with components of the lt peptide chain such as l-cysteine or glutathione, as w ... | 1991 | 1847833 |
| effects of prolonged inhalation of n-formyl-methionyl-leucyl-phenylalanine in rabbits. | on the basis of its potent proinflammatory and spasmogenic effects, n-formyl-methionyl-leucyl-phenylalanine (fmlp), a bacterial oligopeptide, is a putative mediator of bronchoconstriction and airway inflammation during bacterial bronchial infection. however, after an fmlp dose-response curve in rabbits, tachyphylaxis to a second challenge was seen in some rabbits and airway inflammation was absent. this study was designed to reproduce the more prolonged airway exposure to fmlp that may occur dur ... | 1991 | 1885438 |
| modulation of mycotoxin and nitrosamine carcinogenesis by indole-3-carbinol: quantitative analysis of inhibition versus promotion. | the value of chemopreventive agents for reducing human response to mycotoxins and n-nitrosamines remains uncertain, especially since many such agents also can act as tumour promoters. indole-3-carbinol (i3c) from cruciferous vegetables can inhibit dna adduction and hepatocarcinogenesis induced by aflatoxin b1 (afb1) or n-nitroso-diethylamine in trout if given before and with the carcinogen but promotes carcinogenesis when given after initiation. similar results have been obtained with i3c and af ... | 1991 | 1906835 |
| glucose and glutamine metabolism in rat macrophages: enhanced glycolysis and unaltered glutaminolysis in spontaneously diabetic bb rats. | metabolism of glutamine (gln, 2 mm) and glucose (5 mm) was studied in vitro in isolated resident peritoneal macrophages from both normal (bbn) and spontaneously diabetic bb (bbd) rats. the major products from gln were ammonia, glutamate, co2 and to a lesser extent aspartate. glucose decreased (p less than 0.01) the production of ammonia, co2 and aspartate from gln by 34-60%, but had no effect on the amount of glutamate accumulated. the major products from glucose were lactate and to a much lesse ... | 1991 | 1764469 |
| helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (macaca nemestrina) | a new microaerophilic, spirally curved, rod-shaped bacterium was isolated from the gastric mucosa of a pigtailed macaque (macaca nemestrina). the gram-negative cells of this bacterium are oxidase, catalase, and urease positive and strongly resemble helicobacter pylori (campylobacter pylori) cells. like h. pylori, this organism does not metabolize glucose, does not reduce nitrate or produce indole, does not produce h2s from triple sugar iron agar, does not hydrolyze hippurate or esculin, and does ... | 1991 | 1995031 |
| cardiomyopathy and woolly haircoat syndrome of hereford cattle. | | 1991 | 2043089 |
| vestibular papillae of the vulva. lack of evidence for human papillomavirus etiology. | vestibular papillae of the vulva are usually considered as anatomical variants of the vestibular mucosa. clinically, however, they are quite often interpreted as condylomata acuminata and recent studies have suggested that they could be related to human papillomavirus infection. this prompted us to search for human papillomavirus dna using the southern blot hybridization technique, by analyzing biopsy specimens taken from 29 patients who presented with papillae of the vestibular mucosa. human pa ... | 1990 | 2175164 |
| requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. | by analysis of a temperature-sensitive yeast mutant, a heat-shock protein in the matrix of mitochondria, mitochondrial hsp70 (ssc1p), is found to be involved both in translocation of nuclear-encoded precursor proteins across the mitochondrial membranes and in (re)folding of imported proteins in the matrix. | 1990 | 2234077 |
| kinetic study of esterification by immobilized lipase in n-hexane. | the kinetic of the esterification of oleic acid by ethanol catalyzed by immobilized lipase of mucor miehei in n-hexane as a solvent has been completely studied. the kinetics of the reaction are suggested to agree with a ping-pong bi bi mechanism in which only inhibition by excess of ethanol has been identified. values of all apparent kinetic parameters were computed. no evidence of any significant external diffusional limitation which could account for these values has been detected. optimizatio ... | 1990 | 2265698 |
| studies on the mechanism of oxidative phosphorylation. positive cooperativity in atp synthesis. | kinetic and nucleotide binding studies have shown that submitochondrial particles from bovine heart possess three exchangeable binding sites for adp or gdp. in order of decreasing affinity at neutral ph, these sites will be referred to as sites i, ii, and iii, and their respective dissociation constants as ki, kii, and kiii. in oxidative phosphorylation experiments in the presence of saturating amounts of inorganic phosphate, rapid atp (or gtp) synthesis occurred only upon adp (or gdp) binding t ... | 1990 | 2294123 |
| ab-interno neodymium:yag versus erbium:yag laser sclerostomies in a rabbit model. | this study was undertaken to determine whether thermally-induced tissue necrosis was a factor in ab-interno contact-laser sclerostomy failure. a rabbit model was used to compare the continuous-wave neodymium (nd):yag with the pulsed erbium (er):yag laser with respect to such failure. laser energy was focused into a fused-silica fiber optic (400 microns) for the nd:yag laser (12 w; 3 to 5 seconds), and into a single-crystal, uncladded sapphire fiber optic (250 microns) for the er:yag laser (7 to ... | 1992 | 1574289 |
| calcitonin gene-related peptide stimulates irregular spiking and transit of contents in the small intestine of the rat. | | 1990 | 2327270 |