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in-vitro activity of a new penem fce 22101.the in-vitro activity of a new penem fce 22101 was evaluated in comparison with other antimicrobial agents against 966 aerobic and anaerobic gram-negative and gram-positive bacteria. fce 22101 inhibited 90% of enterobacteriaceae, haemophilus influenzae, h. parainfluenzae, h. ducreyi, neisseria gonorrhoeae, branhamella catarrhalis, staphylococcus aureus, staph. epidermidis and group b streptococcus at concentrations of 0.12-4.0 mg/l. pseudomonas aeruginosa was resistant with mic90 of greater than ...19892786518
formation of the 1-(s-glutathionyl)-2,4,6-trinitrocyclohexadienate anion at the active site of glutathione s-transferase: evidence for enzymic stabilization of sigma-complex intermediates in nucleophilic aromatic substitution reactions.formation of the meisenheimer complex or sigma-complex [1-(s-glutathionyl)-2,4,6-trinitrocyclohexadienate] between glutathione (gsh) and 1,3,5-trinitrobenzene (tnb) can be observed at the active sites of isoenzymes 3-3 and 4-4 of rat liver gsh transferase. the spectroscopic properties (uv-visible and cd) of the enzyme-bound sigma-complex are consistent with a 1:1 complex in an asymmetric environment. competitive inhibitors which occupy the gsh binding site (e.g., gamma-l-glutamyl-d,l-2-aminomalo ...19892789999
axonal signals regulate expression of glia maturation factor-beta in schwann cells: an immunohistochemical study of injured sciatic nerves and cultured schwann cells.glia maturation factor-beta (gmf-beta) is a 17 kda protein purified and sequenced from bovine brains. using the monoclonal antibody g2-09 directed against gmf-beta, we previously demonstrated endogenous gmf-beta in astroblasts, schwann cells, and their tumors in culture. in the present study, we have used indirect immunofluorescence microscopy with g2-09 to examine the effects of transection, crush, and regeneration of sciatic nerve on the expression of gmf-beta in schwann cells in situ and to s ...19892795149
granule core loss in eosinophils from a patient with aspirin-induced asthma: an electron microscopic study.aspirin and other antiinflammatory drugs except steroid hormones precipitate asthmatic attacks in some patients with nasal polyps. the respiratory reaction can be severe and life-threatening. the incidence of nasal polyp found in aspirin-induced asthma ranges from 60% to 100%. this case report concerns a 27-year-old japanese man who has suffered from asthma for 4 years and had attacks of asthma after ingestion of antipyretics. he had nasal polyps and sinusitis. the polyps were removed and examin ...19892802266
changes in aldehyde dehydrogenase during rat urinary bladder carcinogenesis.we have reported that normal rat urinary bladder possesses significant amounts of an aldehyde dehydrogenase (class 3 aldh) expressed during hepatocarcinogenesis, but not detectable in normal liver. changes in expression of both liver and bladder aldh during n-butyl-n-(4-hydroxybutyl) nitrosamine (bbn)-induced bladder carcinogenesis were studied. the aldh phenotype was determined at intervals over 42 weeks by histochemical analysis, total aldh activity assays and gel electrophoresis using propion ...19892805229
changes in aldehyde dehydrogenase during rat urinary bladder carcinogenesis.we have reported that normal rat urinary bladder possesses significant amounts of an aldehyde dehydrogenase (class 3 aldh) expressed during hepatocarcinogenesis, but not detectable in normal liver. changes in expression of both liver and bladder aldh during n-butyl-n-(4-hydroxybutyl) nitrosamine (bbn)-induced bladder carcinogenesis were studied. the aldh phenotype was determined at intervals over 42 weeks by histochemical analysis, total aldh activity assays and gel electrophoresis using propion ...19892805229
effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo.the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ...19892813333
effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo.the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ...19892813333
effect of mci-186 on ischemia-induced changes in monoamine metabolism in rat brain.we examined the effects of mci-186 (3-methyl-1-phenyl-2-pyrazolin-5-one), a novel free radical scavenger and an inhibitor of ischemia-induced brain edema, on monoamine metabolism in the brains of both normal and ischemic rats. in normal rats, 3 mg/kg i.v. mci-186, a dose that prevents ischemic brain edema, had no significant effect on brain concentrations of dopamine, norepinephrine, 5-hydroxytryptamine, or their metabolites. after the injection of 5 microliters of 3% polyvinyl acetate into the ...19892815191
effect of mci-186 on ischemia-induced changes in monoamine metabolism in rat brain.we examined the effects of mci-186 (3-methyl-1-phenyl-2-pyrazolin-5-one), a novel free radical scavenger and an inhibitor of ischemia-induced brain edema, on monoamine metabolism in the brains of both normal and ischemic rats. in normal rats, 3 mg/kg i.v. mci-186, a dose that prevents ischemic brain edema, had no significant effect on brain concentrations of dopamine, norepinephrine, 5-hydroxytryptamine, or their metabolites. after the injection of 5 microliters of 3% polyvinyl acetate into the ...19892815191
antibiotic irrigation and the formation of intraabdominal adhesions.the efficacy of antibiotic peritoneal lavage in the prevention of postoperative infection is controversial. the role of intraperitoneally administered cefazolin and tetracycline in the formation of adhesions was studied in the rodent model. thirty-two rats were divided into 3 groups. group 1 underwent midline laparotomy with instillation of 10 ml of normal saline solution. group 2 and group 3 underwent the same procedure with instillation of 0.2 percent saline solutions of cefazolin or tetracycl ...19892817225
glycoside conjugation in microsomes from hepatic and renal carcinoma of man.human hepatoma which had been xenografted into nude mice have been estimated for their ability to catalyze glucuronic acid and glucose conjugation of endogenous compounds and p-nitrophenol. the xenobiotic p-nitrophenol was glucuronidated with a comparable rate in microsomes from human hepatoma, human liver and host liver. with regard to glucuronic acid or glucose conjugation of the endogenous compounds of bile acids, bilirubin and steroid hormones, glucosidation of bile acids was the only conjug ...19872820859
glycoside conjugation in microsomes from hepatic and renal carcinoma of man.human hepatoma which had been xenografted into nude mice have been estimated for their ability to catalyze glucuronic acid and glucose conjugation of endogenous compounds and p-nitrophenol. the xenobiotic p-nitrophenol was glucuronidated with a comparable rate in microsomes from human hepatoma, human liver and host liver. with regard to glucuronic acid or glucose conjugation of the endogenous compounds of bile acids, bilirubin and steroid hormones, glucosidation of bile acids was the only conjug ...19872820859
effects of tilomisole, indomethacin and levamisole on regulation of epstein barr virus-induced b cell proliferation by peripheral blood mononuclear cells from normal individuals and patients with rheumatoid arthritis.when activated in autologous mixed leukocyte reactions (auto-mlr) in vitro, t cells from normal individuals produce a suppressor factor(s) which inhibits the epstein-barr virus (ebv)-induced proliferation of normal b cells. in contrast, t cells from patients with rheumatoid arthritis (ra) are deficient in their ability to generate this suppressor factor in auto-mlr. addition of tilomisole (wy-18,251; 33(p-chlorophenyl)thiazolo[3,2-a]benzimidazole-2-acetic acid) to the auto-mlr (0.1-100 microgram ...19872825478
a one-step preparative method for separating ser 6-phosphorylated hmg 14 from unphosphorylated hmg 14 and in vitro phosphorylation reaction components.while clear evidence exists for the regulation of the phosphorylation of the very basic high mobility group (hmg) and histone chromatin proteins, the physiological role of their phosphorylation remains poorly understood. elucidation of these roles has been difficult, in part, because of the inability to obtain sufficient quantities of purified phosphorylated derivatives. we have used mono s cation-exchange chromatography to prepare milligram quantities of pure ser 6-phosphorylated hmg 14 (ser 6- ...19882839054
initiation and characterization of primary mouse kidney epithelial cultures.primary cultures of murine renal epithelial cells were established from a preparation of proximal tubule fragments. confluent cultures exhibited multiple dome formation, indicating the presence of tight junctions and an intact transcellular transport process. ultrastructural analysis revealed a monolayer of polarized cells, with a sparse but clearly defined microvillar surface facing the growth medium and a basolateral surface attached to the substratum. cultures grown on collagen gels did not s ...19882840432
effect of na+/h+ exchange inhibitors on agonist-induced contraction of rat aorta.a number of vasoconstrictor agonists activate the na+/h+ antiport system in vascular smooth muscle, leading to alkalinization of the cytosol and influx of na+. it is believed that agonist-induced na+/h+ exchange may play an important role in contraction. we have evaluated this hypothesis by determining the effect of inhibition of na+/h+ exchange on phenylephrine (pe)-induced contraction of rat aorta. preincubation of rat aorta with the na+/h+ exchange inhibitor amiloride (0.5 mm) inhibited subse ...19882849662
differential effects of (-)-norepinephrine and of (+/-)-isoproterenol on cardiac contraction, an uptake phenomenon.concentration-dependent inotropic effects of (-)-norepinephrine and of (+/-)-isoproterenol were compared in isometrically contracting guinea pig papillary muscles stimulated at a frequency of 0.2 hz. (-)-norepinephrine (0.1-100 mumol/l) elicited a positive inotropic effect that was not antagonized by carbachol, failed to produce a positive inotropic staircase after resumption of stimulation and shortened relaxation time in a concentration-dependent fashion.(+/-)-isoproterenol had a dual action: ...19882849663
gentamicin inhibits agonist stimulation of the phosphatidylinositol cascade in primary cultures of rabbit proximal tubular cells and in rat renal cortex.a growing body of evidence indicates that aminoglycoside antibiotics interact with phosphoinositides and this has led to the hypothesis that these drugs perturb the phosphatidylinositol (pi) cascade. to test this hypothesis we examined the effect of gentamicin on agonist stimulation of the pi cascade in primary culture of rabbit proximal tubular cells (rptc) and in rat renal cortex. parathyroid (pth) (10(-6) m) stimulated a significant increase in total inositol phosphates, inositol monophosphat ...19882849680
effects of vitamin d3 and related compounds on angiotensin converting enzyme activity of endothelial cells and on release of plasminogen activator from them. 19882854501
effects of vitamin d3 and related compounds on angiotensin converting enzyme activity of endothelial cells and on release of plasminogen activator from them. 19882854501
labelling of liver glutamate by [1-14c]- and [2-14c]-pyruvate as a measure of oxaloacetate-fumarate disequilibrium in the mitochondria. 19852860898
pharmacology of the ruminant gastroduodenal junction. 19852869150
differential translation of the genes encoding the proton-translocating atpase of escherichia coli.translation of the gene for the b subunit of the escherichia coli proton-translocating atpase has been examined. oligonucleotide-directed site-specific mutagenesis was used to mutate certain nucleotides in the intergenic region between unce (c) and uncf (b). one of the changes was predicted to lower the stability of a proposed stem structure which blocked the ribosome binding site of the uncf mrna segment. the result of the mutation is a nearly 3-fold increase in the rate of synthesis of the b p ...19862873137
differential translation of the genes encoding the proton-translocating atpase of escherichia coli.translation of the gene for the b subunit of the escherichia coli proton-translocating atpase has been examined. oligonucleotide-directed site-specific mutagenesis was used to mutate certain nucleotides in the intergenic region between unce (c) and uncf (b). one of the changes was predicted to lower the stability of a proposed stem structure which blocked the ribosome binding site of the uncf mrna segment. the result of the mutation is a nearly 3-fold increase in the rate of synthesis of the b p ...19862873137
homocysteic acid, an endogenous agonist of nmda-receptor: release, neuroactivity and localization. 19862878563
somatostatin 28 and neuropeptide y innervation in the septal area and related cortical and subcortical structures of the human brain. distribution, relationships and evidence for differential coexistence.somatostatin 28- and neuropeptide y-containing innervations were mapped in the human medial forebrain (eight control brains) with immunohistochemistry, using the sensitive avidin-biotin-peroxidase method. peptidergic perikarya and fibers had an extensive distribution: they were densest in the ventral striatum (nucleus accumbens, olfactory tubercle and bed nucleus of the stria terminalis) and infralimbic cortex, of intermediate density in the medial septal area and of lowest density in the dorsal ...19872888048
a mixed endocrine adrenal tumour causing steatorrhoea.a 60 year old man developed steatorrhoea, weight loss, mild diabetes mellitus, labile hypertension and limb cramps. raised plasma concentrations of catecholamines, particularly noradrenaline and a computed tomography-scan showing an adrenal tumour strongly suggested a pheochromocytoma. adrenoreceptor blockade reversed the symptoms, decreased faecal fat, and increased duodenal trypsin to normal concentrations. after adrenalectomy the patient was asymptomatic and there was no steatorrhoea. the blo ...19872890560
isolation and mapping of a polymorphic dna sequence (pmcoc12) on chromosome 14 [d14s20]. 19882899879
cns amyloid proteins in neurodegenerative diseases.the amyloid plaques found in neurodegenerative diseases show considerable morphologic diversity. two amyloidogenic proteins have been isolated from the brains of humans and animals with neurodegenerative diseases--beta-protein from alzheimer's disease (ad) and down's syndrome, and prion protein (prp) from scrapie and creutzfeldt-jakob disease (cjd). using monoclonal antibodies to a synthetic peptide corresponding to a portion of beta-protein and rabbit antiserum to hamster scrapie prp 27-30, we ...19882901696
acquired renal cysts in five-sixths nephrectomized rats: the roles of oxalate deposits in renal tubules and a renotropic factor.the present study was undertaken to see if the oxalate deposits seen in renal tubules are a causative factor in the development of acquired renal cysts in chronic renal failure. thirty 5/6 nephrectomized rats had free access to water containing 8 mg/ml of vitamin c (oxalate precursor) and 20 5/6 nephrectomized rats were given tap water without vitamin c. oxalate deposits were found on microscopy in the renal tubules of vitamin c-treated rats in the 11th and 12th postnephrectomy months; however, ...19892918951
pharmacokinetics of trimetrexate (nsc 352122) in monkeys.the pharmacokinetics of trimetrexate was studied in rhesus monkeys following i.v. bolus, continuous i.v. infusion, oral, and subcutaneous administration. two methods were used to measure drug concentration in plasma, cerebrospinal fluid (csf), and urine: the dihydrofolate reductase inhibition assay, and a reverse phase high-pressure liquid chromatography assay. the pharmacokinetic behavior of trimetrexate was characterized by triexponential plasma disappearance, elimination primarily by biotrans ...19862933145
comparison of human growth hormone levels in children with satisfactory and unsatisfactory growth.sixty-six children with satisfactory growth and 73 with unsatisfactory growth (both sexes) from three different age groups (1 year, 5 years and 10 years) were included in this study. it was observed that the serum concentrations of human growth hormone (hgh) in children with satisfactory growth ranged from 1.00 to 4.00 ng/ml and never exceeded 4.00 ng/ml. in a comparative study there was a significant increase in serum hgh levels in subjects of all age groups who were not growing satisfactorily. ...19872956220
human milk-derived lactoferrin inhibits mitogen and alloantigen induced human lymphocyte proliferation.purified lactoferrin (lf), isolated from human milk, was tested for its effect on human t-lymphocyte proliferative responses to phytohemagglutinin (pha) and to alloantigens in mixed lymphocyte culture. lf inhibited proliferation in both assays in a dose-dependent manner. the suppressive effect was not due to lf mediated cytotoxicity since washing cells that had been pre-incubated with lf restored their proliferative activity. lf was most effective in suppressing the pha response when added withi ...19872963123
stimulation of growth hormone secretion by central administration of atrial natriuretic polypeptide in the rat.intracerebroventricular (icv) injection of alpha-human atrial natriuretic polypeptide (alpha hanp) or alpha-rat anp (0.6 and 3 nmol/rat) elicited an increase in plasma gh levels both in conscious freely moving rats and in urethane-anesthetized rats when given at the trough of spontaneous gh secretion. antiserum specific for rat grf did not affect the plasma gh increase induced by icv injection of alpha hanp. intracerebroventricular injection of alpha anp (3 nmol/rat) failed to stimulate gh secre ...19882966063
[mechanism of ischemic disorders of fatty acid oxidation in heart mitochondria].the article deals with the effect of permanent, temporal and total myocardial ischemia on the oxidation of acetate, hexanoate, palmitoylcarnitine and palmitoyl-coa in isolated rabbit heart mitochondria. all the three models of ischemia in different experimental situations demonstrated a similar degree of fatty acid oxidation suppression independent of the length of acyl residue, the suppression being the greatest during the first hours and in total ischemia. both in the control and in ischemia, ...19852988585
stimulation of a cell-mediated cytotoxic response to felv-induced t cell lymphomas in the cat.nonspecific cell-mediated cytotoxicity was examined in the peripheral blood and spleens of normal and vaccinia virus-infected adult domestic cats. natural cytotoxic (nc)-like cells, as measured by lysis of vaccinia- or hsv-infected, adherent cat tongue cells, were found in both the spleen and peripheral blood of normal, nonimmune cats. cytotoxicity was expressed in a 16-hr assay but not in a 4-hr assay. natural killer (nk)-like cells, as measured by lysis of an felv-induced lymphoid tumor cell l ...19852993416
polyphosphoinositide metabolism in aging human erythrocytes.normal human erythrocytes were fractionated in a density gradient. capacity to metabolize polyphosphoinositides was compared in young (least dense) and old (most dense) cells. polyphosphoinositide synthesis was assessed by following the incorporation of radioactivity from [gamma-32p]atp into the 1-(3-sn-phosphatidyl)-d-myo-inositol 4-phosphate (ptdins4p) and 1-(3-sn-phosphatidyl)-d-myo-inositol 4,5-bisphosphate (ptdins(4,5)p2) of isolated membranes. there was no significant age-dependent change ...19853000548
the epidemiology of the acquired immunodeficiency syndrome. 19863008169
peptidoglycan synthetic activities in membranes of escherichia coli caused by overproduction of penicillin-binding protein 2 and roda protein.penicillin-binding protein (pbp)-2 and the roda protein are known to function in determining the rod shape of escherichia coli cells. peptidoglycan biosynthetic reactions that required these two proteins were demonstrated in the membrane fraction prepared from an e. coli strain that overproduced both of these two proteins and which lacked pbp-1b activity (the major peptidoglycan synthetase activity in the normal e. coli membranes). the cross-linked peptidoglycan was synthesized from udp-n-acetyl ...19863009484
pain, increasing girth in healthy japanese man. 19863021791
pain, increasing girth in healthy japanese man. 19863021791
encephalomyocarditis virus 3c protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the p3 region.all picornaviral peptides are derived by progressive posttranslational cleavage of a giant precursor polyprotein. translation of encephalomyocarditis virus (emc) rna in rabbit reticulocyte extracts produces active viral peptides, including protease 3c, which is responsible for many cleavage reactions within the processing cascade. dna plasmids containing 5' noncoding sequences of emc linked to other portions of the viral genome were constructed and transcribed into rna. like virion rna, the clon ...19863021972
sequence of the genome ends and of the junction between the ends in concatemeric dna of pseudorabies virus.the nucleotide sequences of the termini of the mature pseudorabies virus genome and of the junction between these termini in concatemeric dna were compared. to ensure conservation of unmodified 5' and 3' termini, the end fragments obtained directly (uncloned) from mature viral dna were sequenced. the sequence obtained from 5' and 3' end labeling revealed that whereas the l terminus was blunt ended, the s terminus had a 2-base (gg) 3' overhang. the sequences spanning the junction between the term ...19863023670
vectors for expression and amplification of cdna in mammalian cells: expression of rat phenylalanine hydroxylase.we have constructed two recombinant plasmid vectors for direct expression and amplification of cdna in mammalian cells. each vector carries two dominant selectable markers (the bacterial neo gene and the mouse dhfr gene), a promoter sequence (viral ltr in pav009/a+, and sheep metallothionein promoter in pmt010/a+), a polyadenylation signal sequence, and a bam hi site to allow insertion of cdna. we have used these vectors to prepare recombinant clones for the expression of rat phenylalanine hydro ...19863028740
vectors for expression and amplification of cdna in mammalian cells: expression of rat phenylalanine hydroxylase.we have constructed two recombinant plasmid vectors for direct expression and amplification of cdna in mammalian cells. each vector carries two dominant selectable markers (the bacterial neo gene and the mouse dhfr gene), a promoter sequence (viral ltr in pav009/a+, and sheep metallothionein promoter in pmt010/a+), a polyadenylation signal sequence, and a bam hi site to allow insertion of cdna. we have used these vectors to prepare recombinant clones for the expression of rat phenylalanine hydro ...19863028740
effects of peptide hi on basal and stimulated insulin and glucagon secretion in the mouse.peptide hi (phi) is a peptide with 27 amino acids that is structurally similar to vip (vasoactive intestinal peptide). since phi, like vip, has been demonstrated to occur in intrapancreatic neurons, and since vip earlier was shown to stimulate insulin and glucagon secretion in the mouse, we investigated whether also phi affects islet hormone secretion in this species. phi was thereby injected intravenously at dose levels between 0.5 and 8.0 nmol/kg. it was found that phi did not affect basal lev ...19883047591
[alcohol and endocrinologic homeostasis].in addition to direct toxic effects on endocrine organs chronic alcohol intake affects regulation of endocrine systems by disturbed liver function. as a result in patients with alcohol-induced liver cirrhosis gonadal axis is characterized by low total and free testosterone, elevated estradiol. lh, fsh, and sexual hormone binding globulin and an enhanced conversion of testosterone to estradiol. prolactin also is found to be elevated. the thyrotropic axis is characterised by low t3- und t4- as wel ...19883062942
[the activity of the control de vectores during the 50th anniversary year of the instituto de medicina tropical "pedro kourí"]. 19883067282
[the activity of the control de vectores during the 50th anniversary year of the instituto de medicina tropical "pedro kourí"]. 19883067282
topical mupirocin in the treatment of bacterial skin infections.mupirocin is an investigational topical antibiotic used for treatment and prophylaxis of bacterial skin infections. mupirocin differs from other antibiotics in its synthesis, structure, and mechanism of action. in vitro, mupirocin possesses antimicrobial activity against staphylococci, streptococci, hemophilus influenzae, and neisseria gonorrhoeae. few studies comparing mupirocin to other topical antibiotics are available. initial studies comparing mupirocin to inactive vehicle in the treatment ...19863102197
topical mupirocin in the treatment of bacterial skin infections.mupirocin is an investigational topical antibiotic used for treatment and prophylaxis of bacterial skin infections. mupirocin differs from other antibiotics in its synthesis, structure, and mechanism of action. in vitro, mupirocin possesses antimicrobial activity against staphylococci, streptococci, hemophilus influenzae, and neisseria gonorrhoeae. few studies comparing mupirocin to other topical antibiotics are available. initial studies comparing mupirocin to inactive vehicle in the treatment ...19863102197
regulation of intestinal apolipoprotein b synthesis and secretion by caco-2 cells. lack of fatty acid effects and control by intracellular calcium ion.to investigate the mechanism of control of intestinal apolipoprotein b (apob) secretion, we studied the effects of fatty acids and calcium ionophores on the human intestinal model cell line caco-2. although treatment with various fatty acids (18:1w9, 18:2w6, and 20:5w3) complexed to bovine serum albumin resulted in a dramatic redistribution of apob-100 from the low density and high density lipoproteins to the very low density lipoprotein fraction, there was no effect of any of the fatty acids on ...19883125180
effects of the antimicrobic tiamulin on seven gram-negative bacterial fish pathogens.in vitro and in vivo test were carried out with tiamulin and gram-negative bacterial pathogens of fish. determination of minimum inhibitory concentration for 51 strains of seven species of gram-negative bacterial pathogens showed that only strains of vibrio anguillarum were sensitive at 1.6-6.25 ppm, while the rest of test strains required 25- greater than 100 ppm. control of infection was not achieved when tiamulin was fed for 14 days at 5 or 50 mg/kg to rainbow trout (salmo gairdneri) experime ...19883127604
recovery of mitomycin c-treated mouse 10t1/2 cells during confluent holding.the relationship between cytotoxicity, sister-chromatid exchanges (sce) and the repair of dna crosslinks was studied in mouse 10t1/2 cells during confluent holding following either acute or protracted mmc treatment. no cytotoxic effects were observed with increasing doses of mmc until sce frequencies 1.8 times background levels were induced. protracted mmc treatments were less cytotoxic than acute mmc exposure at doses which yielded similar frequencies of sce. the kinetics of recovery during con ...19883127697
characterization of a-i-containing lipoproteins in subjects with a-i milano variant.the a-i milano variant of apolipoprotein a-i (a-im), by virtue of its arg-173----cys substitution, is capable of forming a disulfide bond with the 77-amino-acid apolipoprotein a-ii polypeptide (a-iis) as well as with itself to produce dimers, a-im/a-iis and a-im/a-im, respectively. a-i-containing lipoproteins (lp): particles with a-ii (lp(a-i with a-11)) and particles without a-ii (lp(a-i without a-ii)) in the plasma of two nonhyperlipidemic a-im carriers were investigated to determine the effec ...19883129016
arachidonic acid, prostaglandin e2 and f2 alpha inhibit leucine degradation in chick skeletal muscle.arachidonic acid (5 microm), prostaglandin e2 (0.28 microm) and f2 alpha (14 microm) inhibited (p less than 0.01) the rates of net leucine transamination, leucine oxidative decarboxylation and total co2 production from leucine in extensor digitorum communis muscles from fed ten-day-old chicks. indomethacin (50 microm) markedly inhibited (p less than 0.01) the rate of pge2 production in the presence of 5 microm arachidonic acid and prevented the inhibition of leucine degradation by arachidonic ac ...19883130060
ethanol and its novel metabolites inhibit insulin action on adipocytes.the effects of ethanol, acetaldehyde, and the two novel metabolites of ethanol 2,3-butanediol and 1,2-propanediol on basal and insulin-stimulated adipocyte lipogenesis and glucose oxidation in vitro were investigated. whereas ethanol and acetaldehyde inhibited both processes at concentrations far greater than those found in alcoholic subjects, the two diols were extremely potent inhibitors of basal and insulin-stimulated adipocyte metabolism at concentrations far below those observed in alcoholi ...19883133260
isolation and characterization of chondroitin 6-sulfate proteoglycans present in the extracellular matrix of rabbit bone marrow.the authors' previous studies showed that the glycosaminoglycans present in rabbit bone marrow were composed of chondroitin 6-sulfate (79%) and hyaluronic acid (16%). immunohistochemically the chondroitin 6-sulfate was demonstrated to be in bone marrow matrix constructing hematopoietic microenvironment. in this study the authors isolated and characterized these glycosaminoglycans in their macromolecular form (i.e., proteoglycans). bone marrow of 3-month-old rabbits was defatted with organic solv ...19883135865
acceleration of starvation- and glycerol-induced myxospore formation by prior heat shock in myxococcus xanthus.the effect of heat shock on myxococcus xanthus was investigated during both glycerol- and starvation-induced development. cells heat shocked at 40 degrees c for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees c. additionally, m. xanthus cells heat shocked prior to glycerol induction formed a greater total number of myxospores when sporulation was complete than did control cells maintained at 30 degrees c. however, in starvation-induce ...19883141380
production and purification of anthrax toxin. 19883148094
effect of different modes of dialysis on serum erythropoietin levels in pediatric patients. a report of the southwest pediatric nephrology study group.the relative importance of erythropoietin (ep) and inhibitors of erythropoiesis in the development of anemia in pediatric patients with end-stage renal disease (esrd) was assessed in 82 patients: 41 treated with peritoneal dialysis (pd) and 41 with hemodialysis (hd). serum ep was determined with a sensitive radioimmunoassay. potential serum inhibition of erythroid (cfu-e) and granulocytic (cfu-gm) progenitor cell growth was assessed using human bone marrow cell cultures. the mean ep level for al ...19883153056
volume overload hypertrophy elicited by cold and its effects on myocardial capillarity.capillarity and fiber cross-sectional areas were measured in the hearts of guinea pigs exposed to cold early during growth. twelve male guinea pigs were kept at 5 +/- 1 degrees c for 4-18 weeks. hearts were perfusion fixed via the aorta with a 2.5% glutaraldehyde, 1% formaldehyde-buffered solution, blocks were cut from left (lv) and right (rv) ventricles, post-fixed in oso4, dehydrated and embedded in spurr medium. blocks were cut transversely to fiber orientation, 0.5 micron thick, stained with ...19853156396
[influence of bovine parathyroid hormone-(1-34) on the contraction of the rat vas deferens and the effect of calcium]. 19853158160
the effect of trypanosoma vivax and trypanosoma congolense infections on the reaction time and semen characteristics in the zebu bull. 19883167554
alpha-lactalbumin production by rat mammary gland: prepartum emergence of resistance to glucocorticoids and progestins.explants of mammary glands from pregnant or lactating rats were shown to differ markedly in their response to glucocorticoid and progestin in terms of alpha-lactalbumin production. tissue from day 14 pregnant rats had low initial tissue levels and basal production of alpha-lactalbumin, and showed a dose-biphasic response to glucocorticoids, with significantly higher production at 3 nm than 300 nm ru26988; the progestin org 2058 inhibited production. in explants from lactating rats (day 1 and day ...19883208993
pregnancy rate and foetal mortality in aleutian disease virus infected mink. 19883223478
[toxicologic properties and the nature of the combined effect of a orthomenthane hydrocarbon mixture]. 19883234846
in situ optical spectroscopy of some systems of biological interest.monitoring the optical absorption or emission spectrum of a condensed phase sample offers information about the supramolecular assembly, packing effects and other features characteristic of the phase that would be missed when one studies solution-state spectra. we have used the technique of photoacoustic spectroscopy to study intact biological specimens, such as algae, parasite cells and the eye lens. such a study has offered information about the status of endogenous hemin in plasmodium cells a ...19883242637
collagen fiber alignment in the human preauricular dermis before and after rhytidectomy--a scanning electron microscopy study.the microarchitecture of collagen fibers in preauricular dermis specimens that were obtained during two-phase rhytidectomy were visualized and recorded using the scanning electron microscope. examination of photomicrographs revealed that both spatial arrangement and degree of convolution of the collagen fibers were completely different in the specimens of the two phases. the results are interpreted in terms of the viscoelastic properties of the skin.19863273035
differential regulation of amyloid-beta-protein mrna expression within hippocampal neuronal subpopulations in alzheimer disease.we have mapped the neuroanatomical distribution of amyloid-beta-protein mrna within neuronal subpopulations of the hippocampal formation in the cynomolgus monkey (macaca fascicularis), normal aged human, and patients with alzheimer disease. amyloid-beta-protein mrna appears to be expressed in all hippocampal neurons, but at different levels of abundance. in the central nervous system of monkey and normal aged human, image analysis shows that neurons of the dentate gyrus and cornu ammonis fields ...19883277189
characterization of the copper-thiolate cluster in yeast metallothionein and two truncated mutants.cu-metallothionein was purified from saccharomyces cerevisiae harboring plasmids containing mutated cup1 metallothionein genes resulting in deletions at the carboxy-terminal end of the polypeptide. the truncated polypeptides are recovered as polypeptides of 35 and 48 residues in length. the cu-s cluster in the wild-type metallothionein and the two truncates were characterized. the truncated proteins, designated t35 and t48, contain 4 and 2 fewer cysteinyl residues, respectively, compared to the ...19883283130
cytotoxic escherichia coli. 19873305326
effects of endotoxemia on pulmonary vascular resistances in unanesthetized sheep.ten experiments were conducted on nine sheep to determine the effects of endotoxemia (1.0 microgram/kg iv over 15 min) on the vascular resistances of two segments of the pulmonary circulation. the first segment (s1) was from the main pulmonary artery to the site in the pulmonary veins corresponding to the pressure measured with a deflated and wedged 7-fr swan-ganz catheter. the second segment (s2) was from the wedge pressure measurement site to the left atrium. endotoxemia caused both pulmonary ...19873308813
activation of the estrogen receptor from n-nitrosomethylurea-induced rat mammary tumors.the activation of the estrogen receptor (er) from n-nitrosomethylurea (nmu)-induced rat mammary tumors was studied in vitro. the activation of the receptor induced by heating of the cytosol containing occupied er was measured by a 3-4-fold increase of receptor binding to nuclei in comparison with the nuclear binding of the nonactivated er. the activation of the er was further shown by alteration of the elution profile from deae-cellulose. a shift of the receptor peak from 234 mm (peak ii, nonact ...19883347053
isolation of a cdna that encodes the peptide core of the secretory granule proteoglycan of rat basophilic leukemia-1 cells and assessment of its homology to the human analogue.it has been previously shown that a single gene is used to encode the peptide core of the extracellular proteoglycan of rat l2 yolk sac tumor cells and the intracellular proteoglycan of rat basophilic leukemia (rbl)-1 cells. in order to determine if the predicted amino acid sequences of these proteoglycans are identical as well as to isolate a full length cdna encoding a rat secretory granule proteoglycan, a cdna library was prepared from rbl-1 cells and screened with the 165-base pair 5'----xmn ...19883366780
[analysis of membrane heterogeneity and non-linearity of summation of postsynaptic potentials in a simulation model of the purkinje cell].propagation and summation of epsp was studied by means of a numerical model of rat purkinje neuron. it was shown that the difference between the membrane resistivity of the soma and dendrites can be determined experimentally by the character of epsp attenuation, while comparing intra- and extracellular recordings. it was also shown that at an increase of synaptic input the nonlinear increase of the potential is more pronounced in the soma that in the synaptic region. the role of this fact for th ...19883370230
structural organization of the mouse mitochondrial malate dehydrogenase gene.structural organization of the mouse mitochondrial malate dehydrogenase (ec 1.1.1.37) gene was determined by analyzing a genomic dna fragment isolated from a cosmid library. the gene is 12,000 base-pairs long and contains nine exons interrupted by eight introns of various sizes. the 5' and 3'-flanking regions, and the exact sizes and boundaries of the exon blocks including the transcription-initiation sites were determined. in the 5'-flanking region, there is neither a tata box nor a caat box. i ...19883379635
[the thymus in exposure to pain].the effect of isotonic solution and microtrauma of the gastrocnemius muscle to the structure and cell composition in various zones of the thymus has been studied on mature white male rats on the first, 7th and 14th days of the experiment. at injection of the isotonic solution (sodium chloride) the part of small lymphocytes decreases and that of the large and middle ones--increases (it is mostly manifested on the 7th day of the experiment). the amount of immature plasma cells increases sharply at ...19883415484
complement component deposition in uteroplacental (spiral) arteries in normal human pregnancy.there is conflicting evidence for the deposition of complement in spiral arteries in normal and abnormal human pregnancies. the immunogold silver staining (igss) technique was used to investigate the distribution of c1q, c3d, c4, c6 and c9 within the spiral arteries of formalin-fixed normal pregnancy hysterectomy specimens ranging in gestational age from 4 to 40 weeks. deposition of complement components studied was observed in all cases suggesting classical pathway activation. reactivity was no ...19873430480
tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells.when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ...19873440028
in vitro studies with melphalan and pediatric neoplastic and normal bone marrow cells.chemotherapeutic agents are used in increasingly high dosages to treat patients with refractory cancers. an in vitro clonogenic assay was used to investigate the effects of melphalan on cultures of human neuroblastoma, ewing's sarcoma, and osteosarcoma cells, as well as on normal bone marrow cells. a 1-hr incubation with 10(-5) m melphalan significantly inhibited tumor colony growth of both fresh neuroblastoma and osteosarcoma cells (p less than 0.01) while ewing's sarcoma cells and normal bone ...19863458679
invasiveness, metastasis, and transplantability of mouse liver nodules.data are presented which show that various mouse liver nodular lesions, such as hyperplastic nodules, hepatocellular adenomas, and hepatocellular carcinomas, differ not only morphologically but also biologically regarding their behavior in the primary and secondary hosts. the hyperplastic nodules grew slowly by expansion without affecting the integrity of the lobular structure. the primary hepatocellular adenomas also grew slowly by expansion but eliminated the histoid liver structure. neither l ...19873472500
department of health and human services, public health service, national institutes of health. recombinant dna advisory committee. minutes of meeting, september 29, 1986. 19873475740
treatment of endemic goitre with iodine and thyroid hormones, alone or in combination. (preliminary report).108 patients with endemic nontoxic goitre have been treated in the field with thyroxine (t4), triiodothyronine (t3), and potassium iodide (ki), singly or in combination, or with placebo. after 6 months of continuous treatment, goitre size decreased significantly in the 7 actively treated groups, but not in the one treated with placebo. the combination of 150 micrograms t4 + 150 micrograms ki daily seemed the most effective treatment, both clinically and by its suppression of the 131i uptake and ...19863486112
possible role of protein phosphorylation in the mitogenic effect of high density lipoproteins on cultured vascular endothelial cells.the implication of protein phosphorylation in the mitogenic action of high density lipoproteins (hdl) on bovine vascular endothelial cells was investigated by incubating endothelial cell cultures in the presence of 32p-labeled phosphoric acid. the incorporation of 32p into proteins was measured after fractionation by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and autoradiography of the gel. in endothelial cells seeded at low density and made quiescent by serum s ...19863486870
short- and long-term effects of angiotensin ii on the rat adrenal zona glomerulosa. a coupled stereological and enzymological study.long-term angiotensin ii administration induced a time-dependent increase in the volume of rat zona glomerulosa cells and in the plasma concentration of aldosterone. cell hypertrophy is coupled with comparable increases in the surface area per cell of mitochondrial cristae and in the activity per cell of 18-hydroxylase. 11 beta-hydroxylase activity was not affected. these findings allow to suggest that the angiotensin-ii-enhanced growth of rat zona glomerulosa is associated with the de novo synt ...19863493913
right hepatic lobectomy for primary lymphoma: a case report and literature review.primary lymphoma of the liver is an extremely rare entity; only eight cases have been reported in the literature. we treated a 34-year-old man with primary hepatic lymphoma by right hepatic lobectomy, including right hemicolectomy the nephrectomy. the histological diagnosis was a small cell diffuse histiocytic lymphoma. details of this case plus that of others in the literature are described in this report.19863534401
isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase.the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ...19863542024
evaluating rapid tests for streptococcal pharyngitis: the apparent accuracy of a diagnostic test when there are errors in the standard of comparison.the evaluation of new rapid antigen detection tests for streptococcal pharyngitis is complicated by the presence of errors in the standard of comparison. even "gold standard" throat cultures are not perfectly accurate. this article is an analysis of how errors in the comparison cultures affect the apparent accuracy of new tests for streptococcal pharyngitis. one of the questions addressed is whether throat cultures performed in a physician's office are accurate enough to use as a standard in a c ...19873553828
cytoplasmic gels from macrophages. evidence for the involvement of four proteins in the calcium-dependent solation of actin networks.a method has been devised to study the influence of ca2+ on the in vitro formation of actin gel networks. under appropriate conditions low-ca2+ cytosolic extracts (less than 1 nm) from macrophages rapidly formed a macromolecular complex composed of actin, filamin, alpha-actinin and two new proteins of 70 kda and 55 kda. [pacaud, m. (1986) eur. j. biochem. 156, 521-530]. increasing concentrations of free ca2+ to 1-2 microm resulted in complete inhibition of the association of 70-kda protein, a pr ...19873569290
cytocidal and toxic effect of various cytostatic drugs on three ascites tumors of the mouse.the cytocidal and toxic effects of four cytotoxic drugs (cy, ddp, vcr, ara-c) were studied using three types of ascites tumors (l 1210, jb-1, eat) growing on three different mouse strains (b6d2f1, akr, nmri). there were considerable differences in the cytocidal effect of the same dose of each drug on the three tumor cell lines; 100% of the l 1210 ascites tumor-bearing animals were permanently cured by a high dose of cy (300 mg/kg) and 30% by ddp (13 mg/kg), while most of the jb-1 and all eat-bea ...19873584212
cytocidal and toxic effect of various cytostatic drugs on three ascites tumors of the mouse.the cytocidal and toxic effects of four cytotoxic drugs (cy, ddp, vcr, ara-c) were studied using three types of ascites tumors (l 1210, jb-1, eat) growing on three different mouse strains (b6d2f1, akr, nmri). there were considerable differences in the cytocidal effect of the same dose of each drug on the three tumor cell lines; 100% of the l 1210 ascites tumor-bearing animals were permanently cured by a high dose of cy (300 mg/kg) and 30% by ddp (13 mg/kg), while most of the jb-1 and all eat-bea ...19873584212
differential examination of bronchoalveolar lavage cells in tobacco cigarette and marijuana smokers.cells derived from bronchoalveolar lavage (bal) were obtained from 19 healthy non-smokers (ns) and from 43 healthy smokers of tobacco and/or marijuana. thirteen subjects smoked tobacco cigarettes only (ts) (mean +/- se: 17.4 +/- 5.5 pack-years). thirty subjects smoked marijuana; of these, 14 smoked marijuana only (ms) (149.1 +/- 102.7 joint-years) and 16 smoked marijuana and tobacco (mts) (43.3 +/- 7.2 joint-years and 18.4 +/- 3.2 pack-years). cell counts were expressed as total number recovered ...19873592403
differential examination of bronchoalveolar lavage cells in tobacco cigarette and marijuana smokers.cells derived from bronchoalveolar lavage (bal) were obtained from 19 healthy non-smokers (ns) and from 43 healthy smokers of tobacco and/or marijuana. thirteen subjects smoked tobacco cigarettes only (ts) (mean +/- se: 17.4 +/- 5.5 pack-years). thirty subjects smoked marijuana; of these, 14 smoked marijuana only (ms) (149.1 +/- 102.7 joint-years) and 16 smoked marijuana and tobacco (mts) (43.3 +/- 7.2 joint-years and 18.4 +/- 3.2 pack-years). cell counts were expressed as total number recovered ...19873592403
effect of protein starvation on protein turnover in liver, oviduct and whole body of laying hens.1. whole body protein turnover rates in white leghorn laying hens were reduced by protein starvation for 7 days, followed by complete restoration by protein repletion for 7 days. 2. protein starvation considerably reduced fractional and absolute rates of protein synthesis both in the liver and, to a greater extent, in the oviduct. 3. it was suggested that a considerable portion of the reduced whole body protein synthesis could be accounted for by the reduced protein synthesis in these organs whe ...19873621897
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 29-1986. an asplenic woman with evidence of sepsis and diffuse intravascular coagulation after a dog bite. 19863724816
the effect of dietary refined sugars and sugar alcohols on renal calcium oxalate deposition in ethylene glycol-treated rats.the effect of administering refined carbohydrates in the diet on calcium oxalate deposition in the kidneys of rats given 1% (v/v) ethylene glycol in their drinking-water was investigated. the rats were given 0, 2.5, 10, 30 or 60% sucrose in the feed (w/w) and/or drinking-water (w/v) or 20% (w/w) starch, glucose, sucrose, fructose, galactose, xylitol or sorbitol in the feed for 3 wk. all of the animals remained healthy over the test period as far as could be assessed by the measurement of 19 plas ...19863744193
quantification of interaction between elf-lf electric fields and human bodies. 19863744391
maternal suprachiasmatic nuclei are necessary for maternal coordination of the developing circadian system.during late fetal and early neonatal life, the maternal circadian system coordinates the timing of a circadian clock in the hypothalamic suprachiasmatic nuclei (scn) to the prevailing light-dark cycle. the role of the maternal scn in the process of maternal coordination was investigated in rats. complete lesions of the maternal scn on day 7 of gestation disrupted rhythms of scn glucose utilization in fetuses and pineal n-acetyltransferase activity in 10-d-old pups. this disruption was probably d ...19863746430
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