Publications

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epr studies of a nonphotosynthetic mutant of rhodospirillum rubrum. 19816271071
incorporation of atp synthetase into long-term stable liposomes of a polymerizable synthetic sulfolipid. 19816271594
crystallographic structure of rhodospirillum molischianum ferricytochrome c' at 2.5 a resolution. 19816279876
antibodies to the f1-atpase of rhodospirillum rubrum and its purified native beta-subunit: inhibition of atp-linked activities in r. rubrum and in lettuce.1. antibodies prepared against the rhodospirillum rubrum f1-atpase (rrf1) and its purified, native-beta-subunit, exhibited cross-reactivity with the following soluble preparations of r. rubrum atpase: rrf0 . f1, rrf1 and the beta-subunit. anti-rrf1, but not anti-beta antibodies, also formed precipitin lines with soluble beta-less rrf1, indicating that antigenic determinants of both the beta-subunit and the other four rrf1-subunits are expressed in the whole rrf1 molecule. both antibodies aggluti ...19816210525
purification and immunological studies of glutathione reductase from rat liver. evidence for an antigenic determinant at the nucleotide-binding domain of the enzyme.glutathione reductase has been purified to homogeneity by a method which is an improvement of an earlier procedure (carlberg, i. and mannervik, b. (1975) j. biol. chem. 250, 5475-5480). the new steps in the purification scheme include affinity chromatography on 2',5' adp-sepharose 4b. antibodies to glutathione reductase from rat liver were raised in rabbits and used for analysis of the enzyme by quantitative 'rocket' immunoelectrophoresis. glutathione reductase from human erythrocytes, porcine e ...19816170346
sequential removal and reconstitution of subunits beta and gamma from a membrane-bound fo.fl-atp synthase. 19816171271
the nucleotide sequence of phenylalanine trna and 5s rna from rhodospirillum rubrum.the complete nucleotide sequence of trnaphe and 5s rna from the photosynthetic bacterium rhodospirillum rubrum has been elucidated. a combination of in vitro and in vivo labelling techniques was used. the trnaphe sequence is 76 nucleotides long, 7 of which are modified. the primary structure is typically prokaryotic and is most similar to the trnaphe of escherichia coli and anacystis nidulans (14 differences of 76 positions). the 5s ribosomal rna sequence is 120 nucleotides long and again typica ...19816174192
biosynthesis of bacterial glycogen: purification and structural and immunological properties of rhodopseudomonas sphaeroides adpglucose synthetase.adpglucose synthetase from the photosynthetic bacterium rhodopseudomonas sphaeroides was purified to greater than 95% purity. the molecular weight of the r. sphaeroides enzyme, as determined by sucrose density gradient ultracentrifugation, was approximately 204,000. the subunit molecular weight of the enzyme based on sodium dodecyl sulfate-gel electrophoresis was 46,000. although the amino acid composition of the enzyme was similar to that found for the enzymes from escherichia coli, salmonella ...19826178721
isolation and purification of an active gamma-subunit of the f0.f1-atp synthase from chromatophore membranes of rhodospirillum rubrum. the role of gamma in atp synthesis and hydrolysis as compared to proton translocation.we have earlier shown that extraction of rhodospirillum rubrum chromatophores with licl removed completely the beta-subunit of their coupling factor atpase complex leaving the other four subunits attached to the membrane (philosoph, s., binder, a., and gromet-elhanan, z. (1977) j. biol. chem. 252, 8747-8752). further treatment of these beta-less chromatophores with libr, under the described optimal conditions, resulted in specific removal of one additional subunit, the gamma-subunit, and both su ...19826181058
chemical modification of the beta-subunit isolated from a membrane-bound fo-f1-atp synthase: modification by 4-chloro-7-nitrobenzofurazan does not inhibit restoration of atp synthesis or hydrolysis. 19826184056
correlations between structural and spectroscopic properties of the high-spin heme protein cytochrome c'.the cytochromes c' are a class of heme proteins whose native spectroscopic properties have been suggested to represent a quantum mechanical admixture of intermediate-(s = 3/2) and high-(s = 5/2) spin states. here features of the cytochrome c' heme environment, as revealed by x-ray crystallographic studies of the dimeric cytochrome c' from rhodospirillum molischianum, are related to the observed spectroscopic properties. the environment of the heme group in cytochrome c' supports the existence of ...19826293536
ribulose-1,5-bisphosphate carboxylase: enzyme-catalyzed appearance of solvent tritium at carbon 3 of ribulose 1,5-bisphosphate reisolated after partial reaction.when ribulose 1,5-bisphosphate is allowed to react with carbon dioxide in tritiated water in the carboxylation reaction catalyzed by ribulose-1,5-bisphosphate carboxylase from rhodospirillum rubrum, the ribulose 1,5-bisphosphate reisolated after partial reaction is found to be labeled. the specific radioactivity of the remaining substrate pool rises during the course of the reaction. experiments in deuterium oxide show that the isotopic label resides on carbon 3. earlier failures to detect this ...19826293539
[generation of the differences of the electric potentials by rhodospirillum rubrum reaction center complexes devoid of the heavy subunit].the electrogenic activity of rhodospirillum rubrum p870 reaction center complexes devoid of the heavy (h) subunit and retaining the light (l) and medium (m) subunits, was studied. the proteoliposomes containing such reaction center complexes were formed by a self-assembly procedure, using soya bean phospholipids. in the presence of ca2+ the reaction center proteoliposomes were incorporated into a phospholipid-impregnated teflon filter separating two solutions of identical composition. after addi ...19826293591
metal coordination centres of class ii cytochromes c.the class ii cytochromes rhodospirillum molischianum cytochrome c', rhodopseudomonas palustris cytochrome c556 and agrobacterium tumefaciens (b2a) cytochrome c556 have been investigated with a variety of spectroscopic techniques. the cytochrome c' was found to be high-spin and the two cytochromes c556 were found to be mainly low-spin and sx-coordinate with the fifth and sixth ligands being histidine and methionine. the implications of the different types of iron coordination are discussed.19826279397
reconstitution of highly purified proton-translocating pyrophosphatase from rhodospirillum rubrum. 19826128256
a sensitive and rapid method for determination of pyrophosphatase activity.a new, rapid and sensitive method of measuring the rate and amount of ppi hydrolysis is described. the method is based on registration of small ph changes, caused by decrease of hydrogen-ion concentration during the pyrophosphatase reaction. with this detected value of hydrogen-ion concentration change and the buffering capacity of each reaction mixture determined by titration, the rate and amount of ppi hydrolysis can be calculated. the results obtained by ph method are in good agreement with v ...19826131564
molecular properties of fumarate reductase isolated from the cytoplasmic membrane of escherichia coli.fumarate reductase, purified from the cytoplasmic membrane of escherichia coli, has been cross-linked with the bifunctional reagent dimethylsuberimidate and shown to exist as an alpha beta dimer of polypeptides of molecular weights 69,000 and 25,000 in a 1:1 molar ratio. the protein has an s20,w of 7.67s and a d20,w of 6.5 x 10(-7) cm2/s. the purified enzyme contained 4-5 mol of nonheme iron and 4-5 mol of acid labile sulfur while the visible absorption spectrum showed a broad peak between 400 a ...19826751504
modification of active site histidine in ribulosebisphosphate carboxylase/oxygenase. 19826800404
photooxidase system of rhodospirillum rubrum iii. the role of rhodoquinone and ubiquinone in the activity of preparations of chromatophores and photoreaction centers.the role of rhodoquinone and ubiquinone in the oxygen photoreducing (photooxidase) activity of rhodospirillum rubrum was investigated. the sole addition of purified rhodoquinone restored photooxidase activity in isolated chromatophores which had been extracted with organic solvents and which were apparently free of secondary acceptor ubiquinone. rhodoquinone also enhanced photooxidase activity in photoreaction center preparations from which secondary ubiquinone seemed to have been removed. those ...19826800786
regulation of rhodospirillum rubrum nitrogenase activity. properties and interconversion of active and inactive fe protein. 19826801038
2-bromoacetylaminopentitol 1,5-bisphosphate as an affinity label for ribulose bisphosphate carboxylase/oxygenase from rhodospirillum rubrum.2-bromoacetylaminopentitol 1,5-bisphosphate (bracnh-pentitol-p2) (an epimeric mixture of 2-bromoacetylamino-2-deoxy-d-ribitol bisphosphate and 2-bromoacetylamino-2-deoxy-d-arabinitol 1,5-bisphosphate) has been synthesized from d-ribulose 1,5-bisphosphate by reductive amination with sodium cyanoborohydride followed by bromoacetylation of the resultant amine with bromoacetyl bromide. under conditions that favor full activation of the enzyme, ribulose bisphosphate carboxylase/oxygenase from rhodosp ...19826801050
reexamination of the binding site for pyridoxal 5'-phosphate in ribulosebisphosphate carboxylase/oxygenase from rhodospirillum rubrum.the high specificity of pyridoxal 5'-phosphate (plp) for an essential lysyl residue of ribulosebisphosphate carboxylase/oxygenase was confirmed, but half-of-sites reactivity was not observed in contrast to an earlier report [robison, p. d., whitman, w. b., waddill, f., riggs, a. f., & tabita, f. r. (1980) biochemistry 19, 4848-4853]. subsequent to reduction with [3h]borohydride and tryptic digestion of the enzyme inactivated by plp, the sole labeled peptide was purified by successive chromatogra ...19826803834
[analysis of the kinetics of transitional processes in biophysical research using a minicomputer]. 19826803850
development and growth of photosynthetic membranes of rhodospirillum rubrum.in cell-free extracts from low-aeration suspensions of rhodospirillum rubrum strain g-9, bacteriochlorophyll a was distributed in two bands after rate-zone sedimentation in sucrose density gradients. from the physicochemical properties of these fractions, it was concluded that the upper band consisted of small membrane fragments, whereas the major band was composed of fragmented vesicular intracytoplasmic membrane (chromatophores). after a pulse with l-[35s]methionine, apparent polypeptide subun ...19826804438
efficiency of light-driven metabolite transport in the photosynthetic bacterium rhodospirillum rubrum.an evaluation of the efficiency of the l-alanine and l-malate transport systems was undertaken with the photosynthetic bacterium rhodospirillum rubrum grown on the amino acid whose uptake was measured. an all-glass apparatus was constructed for measuring transport activity under anaerobic conditions. l-alanine transport activity decreased under conditions of mg2+ depletion. when cells were allowed to become inactive by suspending them in the dark in mg2+-free buffer, full activity could be resto ...19826804443
[reversible effect of intensive light on photobiochemical properties of rhodospirillum rubrum chromatophores].the effect of high intensity (photosynthesis-saturating) light on the optical properties of the bacteriochlorophyll and the light-induced h+ uptake by r. rubrum chromatophores was studied. it was shown that under aerobic conditions illumination causes reversible inhibition (in the dark) of the chromatophore ability for the light-induced uptake of h+, a reversible inhibition of the photosynthetical reaction center function and irreversible bleaching of the antennal bacteriochlorophyll. a kinetic ...19826805518
effect of light intensity and inhibitors of nitrogen assimilation on nh4+ inhibition of nitrogenase activity in rhodospirillum rubrum and anabaena sp.nitrogenase activity in rhodospirillum rubrum was inhibited by nh4+ more rapidly in low light than in high light. furthermore, the nitrogenase of cells exposed to phosphorylation uncouplers was inhibited by nh4+ more rapidly than was the nitrogenase of controls without an uncoupler. these observations suggest that high levels of photosynthate inhibit the nitrogenase inactivation system. l-methionine-dl-sulfoximine, a glutamine synthetase inhibitor, prevented nh4+ from inhibiting nitrogenase acti ...19826807962
photosynthetic bacterial reaction centers: interactions among the bacteriochlorophylls and bacteriopheophytins. 19826808895
[adenylate cyclase from a phototrophic organism]. 19826809439
comparison of active and inactive forms of iron protein from rhodospirillum rubrum.the fe protein of nitrogenase from rhodospirillum rubrum was purified in its active and inactive forms. it is shown that the inactive form exists as a two-subunit modified form of the enzyme as previously reported [ludden & burris (1978) biochem. j. 175, 251--259]. in contrast, the active form exists as a single-subunit unmodified form of the enzyme. the upper subunit (on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis) of the inactive form was shown to contain at least the phosphate ...19826810874
purification and mn2+ activation of rhodospirillum rubrum nitrogenase activating enzyme.the fe protein activating enzyme for rhodospirillum rubrum nitrogenase was purified to approximately 90% homogeneity, using de52-cellulose chromatography and sucrose density gradient centrifugation. activating enzyme consists of a single polypeptide of molecular weight approximately 24,000. atp was required for catalytic activity, but was relatively ineffective in the absence of mg2+. when the concentration of mgatp2- was held in excess, there was an additional requirement for a free divalent me ...19826813313
expression of the activating enzyme and fe protein of nitrogenase from rhodospirillum rubrum.activating enzyme (ae) is responsible for the in vitro activation of inactive fe protein of nitrogenase from rhodospirillum rubrum cells cultured anaerobically with glutamate as the n source. the expression of fe protein and ae was examined in r. rubrum cultured photosynthetically or aerobically on media containing malate as the carbon source. one of the following n sources was used in each culture: glutamate, glutamine, limiting ammonia, high ammonia, glutamate plus histidine, and high ammonia ...19826813314
cytochemical localization and measurement of aerobic 3,3'-diaminobenzidine oxidation reactions in photosynthetically grown rhodospirillum rubrum.photoheterotrophically grown rhodospirillum rubrum mutant c oxidized 3,3'-diaminobenzidine (dab) by two aerobic reactions. one reaction was light dependent. the other respiratory reaction occurred in the dark and could be inhibited by 0.1 m kcn. dab was not oxidized under anaerobic conditions. cytochemical results, obtained by reacting viable cells with dab, indicated that the two reactions were associated with different regions of the cellular membrane system. under dark conditions, oxidized da ...19826813374
pyruvate-dependent diauxic growth of rhodospirillum rubrum in light.when rhodospirillum rubrum mutant c was first exposed to radiant energy after long-term anaerobic dark growth, the cells often exhibited a diauxic growth response. this happened with pyruvate in the medium and when cultures were exposed to a less-than-growth-saturating white light intensity of about 6,460 lx. under the growth-saturating light condition, mutant c photometabolized and growth was not affected by na hypophosphite, an inhibitor of pyruvate fermentation. in lower intensity light, in w ...19826815163
heat activation of the fe protein of nitrogenase from rhodospirillum rubrum.the fe protein of nitrogenase from rhodospirillum rubrum isolated in the inactive form was found to be activated in vitro by heating. this heat activation was dependent upon temperature, ph, and enzyme concentration. during activation by heating, a change in the subunit composition of fe protein was observed on sodium dodecyl sulfate gels. the upper subunit decreases and the lower subunit increased. all components of the modifying group on inactive fe protein appear to be lost upon heat activati ...19826815184
change in subunit composition of the iron protein of nitrogenase from rhodospirillum rubrum during activation and inactivation of iron protein.the subunit composition of the fe protein of nitrogenase from rhodospirillum rubrum during activation and inactivation was investigated. it was found that the upper subunit (on gel electrophoresis) of the two-subunit fe protein was converted into the lower subunit during activation in vitro. when the fe protein was inactivated in vivo by the addition of nh4cl and alpha-oxoglutarate to the cells, a phosphate-labelled upper band appeared. during activation in vitro by the activating enzyme, some o ...19826816216
ribulosebisphosphate carboxylase/oxygenase from rhodospirillum rubrum. 19826818424
protein liganding to the activator cation of ribulosebisphosphate carboxylase.spinach leaf ribulosebisphosphate carboxylase forms a quaternary complex with co2, carboxyarabinitol bisphosphate, and cr2+ or co2+. oxidation of the cation in these complexes produces a protein--cation adduct which is sufficiently stable to be chromatographically isolated after enzyme denaturation. while stoichiometric levels of slowly exchanging cation can be specifically trapped after addition of protein denaturants as well as a vast molar excess of mg2+, neither co2 nor carboxyarabinitol bis ...19826818984
purification and properties of chlorophyllase from greened rye seedlings.1. chlorophyllase [ec 3.1.1.14] was extracted from the acetone-dried powder of the chloroplasts of greened rye seedlings with 1% cholate, and purified 870-fold with a yield of about 30%. the purification procedure was composed of fractionations with acetone and ammonium sulfate, and hydrophobic chromatography on a phenyl-sepharose cl-4b column. 2. the purified enzyme was pure as analyzed by molecular-sieve chromatography and isoelectric electrophoresis. it had an isoelectric point of 4.5 and a m ...19826819291
purification and sequencing of cyanogen bromide fragments from ribulosebisphosphate carboxylase/oxygenase from rhodospirillum rubrum. 19826819814
fermentation and anaerobic respiration by rhodospirillum rubrum and rhodopseudomonas capsulata.rhodospirillum rubrum and rhodopseudomonas capsulata were able to grow anaerobically in the dark either by a strict mixed-acid fermentation of sugars or, in the presence of an appropriate electron acceptor, by an energy-linked anaerobic respiration. both species fermented fructose without the addition of accessory oxidants, but required the initial presence of bicarbonate before fermentative growth could begin. major products of r. rubrum fermentation were succinate, acetate, propionate, formate ...19826798016
peptidoglycan of rhodopseudomonas viridis: partial lack of n-acetyl substitution of glucosamine.a lack of at least 70% of n-acetyl substitution of glucosamine in the glycan strands of the peptidoglycan from the gram-negative bacterium rhodopseudomonas viridis is reported. a disaccharide, very likely glcn beta(1 leads to 4) mur, was observed in hydrolysates of the isolated peptidoglycan. the disaccharide was not observed when peptidoglycan was n-acetylated before hydrolysis. the peptidoglycan of r. viridis was resistant to lysozyme but became sensitive after n-acetylation with acetic anhydr ...19827054141
polar lipids in phototrophic bacteria of the rhodospirillaceae and chromatiaceae families.the polar lipids of photosynthetic purple bacteria of the genera chromatium, thiocapsa, thiocystis, ectothiorhodospira, rhodopseudomonas, rhodospirillum, and rhodomicrobium were analyzed. characteristic compositions of the polar lipids were found for most of the rhodospirillaceae and chromatiaceae species. phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin were the major phospholipids in most species. phosphatidylcholine was present as a major component in all species of the genus e ...19827076618
oxidation of c-type cytochromes by the membrane-bound cytochrome oxidase (cytochrome aa(3)) of blue-green algae.respiratory particles containing an aa(3)-type cytochrome oxidase were prepared from anacystis nidulans, synechocystis 6714, synechococcus lividus, anabaena variabilis, nostoc sp. strain mac, nostoc muscorum, and mastigocladus laminosus. oxidation of c-type cytochromes by membrane preparations of the different blue-green algae was observed using purified cytochromes from horse heart, candida krusei, tuna, saccharomyces oviformis, rhodospirillum rubrum, rhodospirillum molischianum, rhodopseudomon ...198216662253
phosphofructokinase activities in photosynthetic organisms : the occurrence of pyrophosphate-dependent 6-phosphofructokinase in plants and algae.a pyrophosphate-dependent phosphofructokinase (ppi-pfk) activity is detectable in extracts of a wide variety of primitive and advanced plants, the charalean algae, and in the photosynthetic bacterium, rhodospirillum rubrum. angiosperms with extractable ppi-pfk activities 4- to 70-fold higher than the respective atp-pfk activities tend to be succulent and to exhibit cam. even though ppi-pfk activity is not detected in crude extracts of some well known cam plants, e.g. plants in the crassulaceae, ...198316662776
conversion of organic acids to h(2) by rhodospirillaceae grown with glutamate or dinitrogen as nitrogen source.axenic cultures of rhodopseudomonas capsulata. rhodospirillum rubrum, and rhodomicrobium vannielii grown with glutamate as the nitrogen source converted lactate, acetate, and butyrate to h(2) and co(2). conversion rates ranged from 100 to 926 ml h(2) l(r) (-1) day(-1) (where l(r) is the reactor contents), and efficiencies varied from 23 to 100% when grown with n(2), conversion rates up to 760 ml h(2) l(r) (-1) day(-1) and efficiencies up to 100%were achieved. upon aging, cultures appear to rapid ...198318548621
consensus structure and evolution of 5s rrna.a consensus structure model of 5s rrna presenting all conserved nucleotides in fixed positions has been deduced from the primary and secondary structure of 71 eubacterial, archaebacterial, eukaryotic cytosolic and organellar molecules. phylogenetically related groups of molecules are characterized by nucleotide deletions in helices iii, iv and v, and by potential base pair interactions in helix iv. the group-specific deletions are correlated with the early branching pattern of a dendrogram calcu ...19836835839
nitrogen metabolism in the phototrophic bacteria rhodocyclus purpureus and rhodospirillum tenue.studies of the nitrogen nutrition and pathways of ammonia assimilation in rhodocyclus purpureus and rhodospirillum tenue have shown that these two seemingly related bacteria differ considerably in aspects of their nitrogen metabolism. when grown photoheterotrophically with malate as carbon source, r. purpureus utilized only nh4+ or glutamine as sole nitrogen sources and was unable to fix n2. by contrast, r. tenue was found to utilize a variety of amino acids as nitrogen sources and was a good n2 ...19836863218
o-acetylserine sulfhydrylase and s-sulfocysteine synthase activities of rhodospirillum tenue.o-acetylserine sulfhydrylase in cell-free extracts of rhodospirillum tenue was markedly repressed after growth in the presence of sulfide or thiosulfate, whereas s-sulfocysteine synthase activity remained almost unchanged. purification on de52 cellulose resulted in the separation of two proteins: protein i with a molecular weight of 57000 had o-acetylserine sulfhydrylase activity only, while protein ii with a molecular weight of 46000 had s-sulfocysteine synthase activity in addition. the activi ...19836615127
the effects of light and oxygen on membrane assembly in the photosynthetic bacteria. 19836617969
interaction of ribulose bisphosphate carboxylase/oxygenase with 2-carboxyhexitol 1,6-bisphosphates.2-c-carboxy-d-glucitol 1,6-bisphosphate (cgbp) and 2-c-carboxy-d-mannitol 1,6-bisphosphate (cmbp) have been synthesized, isolated, and the structures of these compounds and the derived lactones elucidated by nmr spectroscopy and periodate oxidation. both carboxyhexitol bisphosphates, which are homologs of the transition state analog 2-c-carboxy-d-arabinitol 1,5-bisphosphate, exhibit competitive inhibiton of ribulose bisphosphate carboxylase/oxygenase (ec 4.1.1.9) isolated from spinach (spinacia ...19836573158
species variation in kinetic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase.several kinetic parameters of ribulose-1,5-bisphosphate (rubp) carboxylase/oxygenase from different species were measured and compared. the co2/o2 specificity (vcko/vokc) was found to be about 80 in the enzymes from several c3 species and two c4 species. specificity values of 58 and 70, respectively, were found in enzymes from the c4 plants setaria italica and sorghum bicolor. two enzymes from cyanobacteria had values of about 50. substitution of mn2+ for mg2+ reduced the co2/o2 specificity by a ...19836582802
rna ligase in bacteria: formation of a 2',5' linkage by an e. coli extract.ligase activity was detected in extracts of escherichia coli, clostridium tartarivorum, rhodospirillum salexigens, chromatium gracile, and chlorobium limicola. ligase was measured by joining of trna halves produced from yeast ivs-containing trna precursors by a yeast endonuclease. the structure of trnatyr halves joined by an e. coli extract was examined. the ligated junction is resistant to nuclease p1 and rnaase t2 but sensitive to venom phosphodiesterase and alkaline hydrolysis, consistent wit ...19836347395
derepression of the synthesis of d-ribulose 1,5-bisphosphate carboxylase/oxygenase from rhodospirillum rubrum.the synthesis of ribulose 1,5-bisphosphate carboxylase/oxygenase in rhodospirillum rubrum was greatly influenced by the conditions of culture. when grown photolithotrophically in an atmosphere containing low levels of co2 (1.5 to 2%), enzyme synthesis was derepressed, with the result that the enzyme comprised up to 50% of the soluble protein of the cells as determined by immunological quantitation. this response was not observed when r. rubrum was grown photolithotrophically in an atmosphere of ...19836401286
overproduction of nitrogenase by nitrogen-limited cultures of rhodopseudomonas palustris.rhodopseudomonas palustris cells grown on limiting nitrogen produced four- to eightfold higher nitrogenase specific activity relative to cells sparged with n2. the high activity of n-limited cells was the result of overproduction of the nitrogenase proteins. this was shown by four independent techniques: (i) titration of the mo-fe protein in cell-free extracts with fe protein from azotobacter vinelandii; (ii) direct detection of the subunits of mo-fe protein by sodium dodecyl sulfate-polyacrylam ...19836402491
identification of two distinct lactate dehydrogenases in rhodospirillum rubrum.the activities of pyridine nucleotide-independent d- and l-lactate dehydrogenases were detected in membranes from rhodospirillum rubrum grown under aerobic and phototrophic conditions. crossed immunoelectrophoretic analysis revealed two antigenically distinct enzymes that were further distinguished by specificity for d- and l-stereoisomers of lactate and by the sensitivity of the d-lactate dehydrogenase to inhibition by oxamate and oxalate.19836402502
[purification of adenyl cyclase from the phototrophic bacterium rhodospirillum rubrum]. 19836403060
adenine nucleotide levels in and nitrogen fixation by the cyanobacterium anabaena sp. strain 7120.adenine nucleotide levels were determined in whole filaments of anabaena sp. 7120 grown under different n2-fixing or non-n2-fixing conditions. these were compared with levels in isolated heterocysts, rhodospirillum rubrum, and azotobacter vinelandii. adenine nucleotides in whole filaments of anabaena sp. do not reflect the energetic expense of n2 fixation as they do in r. rubrum and a. vinelandii. however, adenine nucleotide levels in heterocysts were similar to the levels found in n2-fixing r. ...19836403506
isolation and sequencing of an active-site peptide from rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase after affinity labeling with 2-[(bromoacetyl)amino]pentitol 1,5-bisphosphate.2-[(bromoacetyl)amino]pentitol 1,5-bisphosphate was reported to be a highly selective affinity label for ribulosebisphosphate carboxylase/oxygenase from rhodospirillum rubrum [fraij, b., & hartman, f. c. (1982) j. biol. chem. 257, 3501-3505]. the enzyme has now been inactivated with a 14c-labeled reagent in order to identify the target residue at the sequence level. subsequent to inactivation, the enzyme was carboxymethylated with iodoacetate and then digested with trypsin. the only radioactive ...19836404301
action of chlorophyllase purified from rye seedlings on light-harvesting bacteriochlorophyll of chromatophores and spheroplasts from rhodospirillum rubrum.1. the chlorophyllase [ec 3.1.1.14] purified from greened rye seedlings hydrolyzed the bacteriochlorophyll isolated from rhodospirillum rubrum, but not the pigment bound to the membrane of chromatophores or spheroplasts from the bacterium. 2. acetone, if added at such concentrations that the bound bacteriochlorophyll would not be solubilized, enabled the enzyme to hydrolyze the bound pigment. the acetone concentrations required for half the maximum hydrolysis rates were 16% with chromatophores a ...19836404894
hybridization of cloned rhodopseudomonas capsulata photosynthesis genes with dna from other photosynthetic bacteria.the homology of rhodopseudomonas capsulata dna segments carrying photosynthesis genes with sequences present in total dna from certain other photosynthetic and non-photosynthetic bacterial species was determined by hybridization. r. capsulata dna fragments that carry loci for production of peptide components of the photosynthetic reaction center and light-harvesting i antenna complex were found to hybridize to dna from some photosynthetic species. however, fragments that carry carotenoid or bact ...19836406432
[interaction of redox mediators with chromatophores of the photosynthetic bacterium rhodospirillum rubrum]. 19836408397
incorporation of adenine into the modifying group of inactive iron protein of nitrogenase from rhodospirillum rubrum.adenine was fed to cells of rhodospirillum rubrum grown on glutamate. the adenine was found to be incorporated into the modifying group of the inactive form of iron protein. adenine labelled in the 8-position ([8-3h]adenine) and the 2-position ([2-3h]adenine) was specifically incorporated into the electrophoretic 'upper-band' subunit of iron protein. incorporation of label from the 2-position into many proteins was observed if histidine was not present in the medium. label was removed by the act ...19836409076
ribulose bisphosphate carboxylase/oxygenase in toluene-permeabilized rhodospirillum rubrum.toluene-permeabilized rhodospirillum rubrum cells were used to study activation of and catalysis by the dual-function enzyme ribulose bisphosphate carboxylase/oxygenase. incubation with co2 provided as hco3-, followed by rapid removal of co2 at 2 degrees c and subsequent incubation at 30 degrees c before assay, enabled a determination of decay rates of the carboxylase and the oxygenase. half-times at 30 degrees c with 20 mm-mg2+ were 10.8 and 3.7 min respectively. additionally, the concentration ...19836409101
architectonic natures of proteins bound to rhodospirillum rubrum chromatophores as detected by trypsin treatment and sonication. 19836409893
structure of the extracellular ferredoxin from rhodospirillum rubrum: close similarity to clostridial ferredoxins.the amino acid sequence of an [8fe-8s] ferredoxin isolated from the culture medium of rhodospirillum rubrum, a photosynthetic purple non-sulfur bacterium, was determined by a combination of various conventional procedures. the sequence was a-y-k-i-e-e-t-c-i-s-c-g-a-c-a-a-e-c-p-v-n-a-i-e-q-g-d-t-i-f-v-v-n-a-d-t-c-i-d-c - g-n-c-a-n-v-c-p-v-g-a-p-v-a-e (55 amino acid residues). it lacked methionine, leucine, histidine, arginine, and tryptophan. the molecular weight was calculated to be 5,568 exclud ...19836411697
the role of mg2+ and mn2+ in the enzyme-catalysed activation of nitrogenase fe protein from rhodospirillum rubrum.the activation of the fe protein of nitrogenase (rr2) from glutamate-grown rhodospirillum rubrum by activating enzyme (ae) was investigated. ae is confirmed to have mr about 20 000 and is shown to operate catalytically. there is a role in activation for metal-ion-atp, which can be met by either mnatp or mgatp. there is also a site of action for free metal ions. this site prefers mn2+ (apparent kd approx. 20 microm) over mg2+ (apparent kd approx. 20 mm) by a factor of 1000-fold. non-activated rr2 ...19836412690
[intramolecular dynamics and electron transfer in photosynthetic reaction centers. a study using luminescence].the temperature dependences of fluorescence and phosphorescence spectra maxima of chromophor labels--endogenic (tryptophan) and exogenic (eosinisothiocyanate)--were measured for the preparations of photosynthetic membranes and reaction centers from rhodospirillum rubrum. it was found that the dipole mobility of protein-lipid matrix in the vicinity of the chromophores intensified markedly with a temperature rise from 150 to 300k resulting in the corresponding relaxation time tau r decrease from 1 ...19836413837
[dicyclohexylcarbodiimide as an inhibitor of light- and pyrophosphate-induced formation of membrane potential in chromatophores of purple bacteria].n,n'-dicyclohexylcarbodiimide (dccd) suppresses the uptake of penetrating tetraphenylborate anions by rhodospirillum rubrum chromatophores during cyclic and non-cyclic electron transfer and atp and pp i hydrolyses. the photochemical activity of the bacteriochlorophyll reaction centers of the chromatophores in insensitive to dccd. this supports the view that dccd inhibits the electron transfer between the primary and secondary quinones of the photosynthetic chain. incorporation of the chromatopho ...19836414533
inhibition of ribulose bisphosphate carboxylase by substrate ribulose 1,5-bisphosphate.substrate ribulose bisphosphate is a potent and a weak inhibitor of the rate of co2/mg2+ activation in the carboxylase purified from spinach leaves and rhodospirillum rubrum, respectively. at 2 degrees c, the concentration of ribulose bisphosphate required for 50% inhibition of the initial rate of co2/mg2+ activation was less than 0.4 microm for the spinach enzyme, but between 67 and 270 microm for the r. rubrum carboxylase. activator 14co2 trapping experiments demonstrated that ribulose bisphos ...19836417133
uptake of methionine sulfoximine by some n2 fixing bacteria, and its effect on ammonium transport.the n2 fixing bacteria klebsiella pneumoniae, azospirillum brasilense, rhodopseudomonas sphaeroides and rhodospirillum rubrum, but not azotobacter vinelandii accumulate the glutamine analogue methionine sulfoximine in the cell. in the accumulating cells methionine sulfoximine inhibits ammonium transport. accumulation and inhibition are prevented by glutamine.19836418571
chemical nature of protein complex of photoreaction unit including reaction center in chromatophores of photosynthetic bacterium, rhodospirillum rubrum, as detected by successive dissociation method.reaction center of chromatophores of rhodospirillum rubrum consists of three kinds of protein, h-, m-, and l-subunit, and is bound with many other kinds of protein to form a larger protein complex (pru; photoreaction unit), which contains all the bacteriochlorophyll. in the present study, purified pru was dissociated in a stepwise manner in the presence of various mixtures of lithium dodecyl sulfate, sodium cholate and/or sodium deoxycholate, and separated into five, smaller protein complexes (p ...19836423620
inorganic pyrophosphate-driven atp-synthesis in liposomes containing membrane-bound inorganic pyrophosphatase and f0-f1 complex from rhodospirillum rubrum.ppi driven atp synthesis has been reconstituted in a liposomal system containing the membrane-bound energy-linked ppiase and coupling factor complex, both highly purified from rhodospirillum rubrum. this energy converting model system was made by mixing both enzyme preparations with an aqueous suspension of sonicated soybean phospholipids and subjecting to a freeze-thaw procedure. in the presence of adp, mg2+, pi and ppi the system catalyzed phosphorylation by up to 25 nmol atp formed x mg prote ...19836132837
evidence for a glutamine synthetase-chromatophore association in the phototroph rhodospirillum rubrum: purification, properties, and regulation of the enzyme.the characteristics of soluble and membrane-bound glutamine synthetase (gs) from rhodospirillum rubrum were compared with those of the enzyme located in situ (measured in detergent-treated cells). the results suggest that in vivo gs may be associated with, or bound to, the chromatophore membranes. gs was found to reversibly associate and dissociate from purified chromatophores as a function of the ionic strength of the buffer or the mg2+ concentration. solubilized gs was purified to homogeneity ...19836132914
[effect of 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, a ubiquinone analog, and sh-reagents on the electrogenic function of pyrophosphatase from rhodospirillum rubrum chromatophores].inorganic pyrophosphate-induced membrane potential generation in rhodospirillum rubrum chromatophores is inhibited by 2,5-dibromo-2-methyl-6-isopropyl-p-benzoquinone (dbmib). the inhibition is increased by menadione and dithionite and is not relieved by ascorbate; this effect is probably due to a displacement of the intrachromatophore quinones by dbmib. the sh-reagents, n-ethylmaleimide and p-chloromercurybenzoate at high concentrations inhibit membrane potential generation driven by inorganic p ...19836135455
h2 metabolism in photosynthetic bacteria and relationship to n2 fixation.the photosynthetic bacteria can evolve h2 in the light through a nitrogenase-mediated reaction. the nitrogenase enzyme in the photosynthetic bacteria is similar to other nitrogenases. it is made of two soluble components: a) the fe protein (dinitrogenase reductase or component ii) which receives electrons from ferredoxin, and b) the mo-fe protein (dinitrogenase or component i) on which the substrates (including protons) are reduced. in photosynthetic bacteria, the physiological regulation of nit ...19836139053
individual 1h-nmr assignments for the heme groups and the axially bound amino acids and determination of the coordination geometry at the heme iron in a mixture of two isocytochromes c-551 from rhodopseudomonas gelatinosa.this paper describes chemical and physicochemical studies of two small isocytochromes c-551 (approx. 9000 dalton) from rhodopseudomonas gelatinosa. in spite of numerous amino acid substitutions in the n-terminal half of the sequence the two isoproteins could not be separated by the procedures used, presumably because they have identical size, charge and isoelectric points. individual assignments of the 1h-nmr lines of heme c and the axial ligands to the heme iron were therefore obtained by nucle ...19836297597
nitrogen fixation and ammonia switch-off in the photosynthetic bacterium rhodopseudomonas viridis.rhodopseudomonas viridis atcc 19567 grows by means of nitrogen fixation in yeast extract-n2 or nitrogen-free medium when sparged with 5% co2 and 95% n2 in the light at 30 degrees c. acetylene reduction assays for nitrogenase activity revealed an initially high level of activity during early-logarithmic growth phase, a lower plateau during mid- to late-logarithmic phase, and a dramatic reduction of activity at the beginning of the stationary phase. when viewed by electron microscopy, nitrogen-fix ...19836305906
two types of essential carboxyl groups in rhodospirillum rubrum proton atpase.two different types of essential carboxyl groups were detected in the extrinsic component of the proton atpase of rhodospirillum rubrum. chemical modification of r. rubrum chromatophores or its solubilized atpase by woodward's reagent k resulted in inactivation of photophosphorylating and atpase activities. the apparent order of reaction was nearly 1 with respect to reagent concentration and similar k1 were obtained for the soluble and membrane-bound atpases suggesting that inactivation was asso ...19836307154
characterization of a rhodospirillum rubrum plasmid: loss of photosynthetic growth in plasmidless strains.a single plasmid of 55 kilobases was found in crude cell lysates of each of nine strains of rhodospirillum rubrum. restriction endonuclease analysis showed identical fragment patterns with a given nuclease for all plasmids except one, for which an additional ecori site was observed. elimination of the plasmid required that the cells be passaged several times in 25 mm calcium-containing medium, followed by at least two passages under photosynthetic growth conditions in low-calcium medium before t ...19836313617
the dibromothymoquinone effect on membrane potential generation in rhodospirillum rubrum chromatophores.2,5-dibromo-3-methyl-6-isopropyl benzoquinone (dbmib) inhibits the light-dependent membrane potential generation in rhodospirillum rubrum chromatophores. the inhibition is relieved by electron donors and is obviously due to oxidation of the photosynthetic electron transfer chain components. in addition, high dbmib concentrations elicit another effect probably caused by disruption of quinone functions in chromatophores. however, in quinone-depleted chromatophores and proteoliposomes containing th ...19836316108
identification of nitrogenase and carboxylase genes in the photosynthetic bacteria and cloning of a carboxylase gene from rhodopseudomonas sphaeroides.the presumptive genes for the ribulose 1,5-bisphosphate carboxylase large subunit and for nitrogenase-specific components from rhodopseudomonas sphaeroides and several other photosynthetic bacteria were identified and located by interspecific probing. restriction digests of r. sphaeroides genomic dna were hybridized under stringent conditions to cloned dna from rhodospirillum rubrum (plasmid prr2119 carrying the carboxylase gene) and klebsiella pneumoniae (psa30 carrying the nitrogenase genes). ...19836319239
immunochemical relationship of the major outer membrane protein of rhodopseudomonas sphaeroides 2.4.1 to proteins of other photosynthetic bacteria.immunoblots of sodium dodecyl sulfate-polyacrylamide gels derived from outer membrane preparations of various strains of rhodopseudomonas sphaeroides revealed polypeptides which cross-reacted with antibody directed against the major outer membrane protein of r. sphaeroides 2.4.1. immunochemical quantitation of the major outer membrane protein of strain 2.4.1 showed approximately 5.5 x 10(4) molecules per cell whether cells were grown chemoheterotrophically or photoheterotrophically. rhodospirill ...19836188744
neutron small angle scattering of matched proteoliposomes with incorporated f0f1 atpase complex from rhodospirillum rubrum fr1. an approach to the structure of membrane proteins in their natural environment.purified f0f1 atpase from rhodospirillum rubrum fr1 has been incorporated into lipid vesicles from the partially deuterated phospholipid dimyristoylglycerophosphocholine (dmpc-d54). these proteoliposomes were able to carry out energy transducing reactions. the incorporation of the membrane protein was controlled by freeze fracture electron microscopy. a method for structural research of the membrane protein in its natural environment has been developed by means of neutron small angle scattering. ...19836195064
n-terminal sequences of subunits l and m of the photosynthetic reaction centre from rhodospirillum rubrum g-9+. separation of the subunits by gel filtration on hydroxypropylated sephadex g 100 in organic solvents.a new method has been developed by which subunits l and m of the photosynthetic reaction centre from rhodospirillum rubrum g-9+ can be obtained in pure form, starting form freeze-dried chromatophore membranes. the method employs extraction into a mixture of chloroform/methanol and gel permeation chromatography on a column of hydroxypropylated sephadex g 100. cross-contamination of the purified subunits was less than 5% (mol/mol), as estimated by manual edman degradation. automated edman degradat ...19836199280
the interaction of 4-chloro-7-nitrobenzofurazan with rhodospirillum rubrum chromatophores, their soluble f1-atpase, and the isolated purified beta-subunit.atp synthesis and hydrolysis by rhodospirillum rubrum chromatophores as well as the soluble rrf1-atpase activity are inhibited by 4-chloro-7-nitrobenzofurazan (nbd-c1) in a dithiothreitol-reversible manner. using the method earlier developed in these chromatophores to remove specifically the beta-subunit from their membrane-bound rrf1 leaving all other subunits attached to the resulting inactive beta-less chromatophores (philosoph, s., binder, a., and gromet-elhanan, z. (1977) j. biol. chem. 252 ...19836219996
the interaction of carboxyl group reagents with the rhodospirillum rubrum f1-atpase and its isolated beta-subunit.the carboxyl group reagents dicyclohexylcarbodiimide (dccd) and n-ethoxycarboxyl-2-ethoxy-1,2-dihydroquinoline (eedq) inactivate the soluble rhodospirillum rubrum f1-atpase (rrf1). the inactivation is both time- and concentration-dependent and also ph-dependent, being more marked at acid ph. under the same conditions, n-ethyl-5-phenylisoxazolium 3'-sulfonate causes almost no inactivation of the rrf1-atpase. complete inhibition of the enzyme activity requires the binding of 1 mol of dccd/mol of r ...19836219997
[reconstruction of highly purified proton-translocating pyrophosphatase from rhodospirillum rubrum].using the freezing-thawing procedure, a highly purified preparation of ppase from r. rubrum chromatophore membranes was incorporated into soybean phospholipid liposomes. the activity of reconstituted ppase was increased in the presence of the uncoupler, fccp, and the antibiotics, valinomycin (+kcl) and nigericin (+kcl). oligomycin did not exert any inhibiting action, while imidodiphosphate and naf significantly decreased the activity of the ppase incorporated into the liposomes. preincubation of ...19836226320
response of soluble and membrane-bound f1 atpase of rhodospirillum rubrum to the photoaffinity label 8-azido atp.soluble atpase (f1 atpase) and membrane-bound atpase (chromatophores) of rhodospirillum rubrum have been photoaffinity labeled with 8-azidoadenosine 5'-triphosphate (8-n3atp). the specific binding of 8-n3atp to nucleotide binding sites of the chromatophore-bound enzyme is established by competition experiments and it is suggested that hydrolysis and phosphorylation involve the same nucleotide binding sites. the experimental results also indicate that the function of the coupling factor f1 is inf ...19836237651
preparative purification of the subunits of chloroplast and rhodospirillum rubrum coupling factors by flat-bed electrofocusing in granulated gels.a rapid method for the preparative purification of the subunits of oligomeric proteins like chloroplast and rhodospirillum rubrum coupling factors is presented. it involves the dissociation of the protein in urea and the separation of its subunits by isoelectric focusing in flat-beds of sepharose cl-4b or sephadex g-75 superfine, in the presence of urea and in an overnight run. using this procedure in the ph range 5-7, we have purified to homogeneity the alpha, beta and delta subunits of chlorop ...19846240506
polyadenylated mrna from the photosynthetic procaryote rhodospirillum rubrum.total cellular rna extracted from rhodospirillum rubrum cultured in butyrate-containing medium under strict photosynthetic conditions to the stationary phase of growth has been fractionated on an oligodeoxythymidylic acid-cellulose column into polyadenylated [poly(a)+] rna and poly(a)- rna fractions. the poly(a)+ fraction was 9 to 10% of the total bulk rna isolated. analysis of the poly(a)+ rna on a denaturing urea-polyacrylamide gel revealed four sharp bands of rna distributed in heterodisperse ...19846199342
reconstruction of photosynthetic, cyclic electron transport system from photoreaction unit, ubiquinone-10 protein, cytochrome c2 and polar lipids purified from rhodospirillum rubrum.it was previously reported that in chromatophores of rhodospirillum rubrum, reaction center, which consists of three kinds of protein (mm, about 78k), is a small fragment of a large protein complex (pru; photoreaction unit), which contains six other kinds of protein including light-harvesting bacteriochlorophyll protein, has mm of about 700k and is free of phospholipid [j. biochem. 86, 1211-1224 (1979); 94, 1815-1826 (1983(]. in the present study, the photosynthetic, cyclic electron transport sy ...19846325401
derepression of nitrogenase by addition of malate to cultures of rhodospirillum rubrum grown with glutamate as the carbon and nitrogen source.rhodospirillum rubrum grown in continuous culture with glutamate as the sole fixed c and n source produced no nitrogenase, and the cultures were characterized by high extracellular ammonium concentrations. addition of organic acids derepressed nitrogenase. glutamate dehydrogenase, glutamine synthetase, glutamate synthase, malate dehydrogenase, nitrogenase, and ammonium were assayed before and after malate addition.19846145702
membrane-bound inorganic pyrophosphatase. 19846151664
the shape of ribulose bisphosphate carboxylase/oxygenase in solution as inferred from small angle neutron scattering.small angle neutron scattering of both activated and deactivated hexadecameric ribulose bisphosphate carboxylase/oxygenase from spinach revealed that its structure in solution very closely resembles that determined for the deactivated crystalline enzyme from tobacco (baker, t.s., eisenberg, d., and eiserling, f. (1977) science 196, 293-295). scattering from both forms of the enzyme in h2o most closely fits that expected from a hollow sphere with an outer radius of 56.4 a and inner radius of 14.3 ...19846423629
x-ray diffraction studies on chromatophore membrane from photosynthetic bacteria. ii. comparison of diffraction patterns of photosynthetic units from various purple bacteria.comparative x-ray diffraction studies, in conjunction with infrared absorption spectroscopy, were performed on chromatophores isolated from various purple photosynthetic bacteria in order to achieve a better understanding of the molecular structure of the photosynthetic unit. purple non-sulfur bacteria used were rhodospirillum rubrum, rhodospirillum molischianum, rhodopseudomonas sphaeroides, and rhodopseudomonas palustris. chromatophores of chromatium vinosum, as a typical example of purple sul ...19846425275
x-ray diffraction studies on photosystem i fragments from a blue-green alga, anabaena variabilis, and spinach.photosystem i fragments were prepared from thylakoid membranes of a blue-green alga (anabaena variabilis) and spinach by treatment with a detergent, triton x-100. equatorial x-ray diffraction patterns were recorded on films for oriented specimens of thylakoid membranes and photosystem i fragments. the thylakoid membranes and photosystem i fragments gave essentially the same equatorial diffraction patterns in both anabaena variabilis and spinach, indicating that the major x-ray scatterers in thes ...19846425276
effect of ammonia, darkness, and phenazine methosulfate on whole-cell nitrogenase activity and fe protein modification in rhodospirillum rubrum.a procedure for the immunoprecipitation of fe protein from cell extracts was developed and used to monitor the modification of fe protein in vivo. the subunit pattern of the isolated fe protein after sodium dodecyl sulfate-polyacrylamide gel electrophoresis was assayed by coomassie brilliant blue protein staining and autoradiographic 32p detection of the modifying group. whole-cell nitrogenase activity was also monitored during fe protein modification. the addition of ammonia, darkness, oxygen, ...19846427184
intermediates in the ribulose-1,5-bisphosphate carboxylase reaction.at least two intermediates of the d-ribulose-1,5-bisphosphate carboxylase/oxygenase (ec 4.1.1.39) reaction were liberated in detectable amounts when the functioning enzyme from rhodospirillum rubrum was quenched in acid. using substrate labeled with 32p in c-1, [32p]orthophosphate (pi) was found when the quenched solution was rapidly processed for extraction of pi as the acid molybdate complex. reaction with sodium borohydride under mildly alkaline conditions immediately after acid quenching of ...19846427222
preliminary x-ray diffraction study of ribulose-1,5-bisphosphate carboxylase from rhodospirillum rubrum.crystals from the dimeric enzyme ribulose-1,5-bisphosphate carboxylase of the photosynthetic bacterium rhodospirillum rubrum have been obtained from the gene product expressed in escherichia coli. the crystals are of the quarternary complex comprising enzyme: activator co2 (as a carbamate): mg2+: 2- carboxyarabinitol -1,5-bisphosphate (as a transition state analog). x-ray diffraction photographs show symmetry consistent with space group p4(1)2(1)2 or the corresponding enantiomorphic space group. ...19846427471
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