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recent advances in diagnosis and treatment of cat scratch disease.the cause of cat scratch disease (csd), first described in france in 1950 and in the united states in 1951, was unknown until 1983 when the bacterium in lymph nodes was detected using a warthin-starry silver stain. afipia felis has been an infrequent cause of csd since1988, when this gram-negative bacterium was first isolated from 10 patients with csd. in 1992 bartonella organisms were isolated from immunocompetent and immunocompromised patients. an indirect fluorescent antibody test to detect b ...200011095849
comparison of metal complexes between depyruvamide bleomycin and bleomycin: an important effect of axial donor on metal coordination and oxygen activation. 200088941
comments on references to the american board of psychiatry and neurology in an article. 20023369584
[the late david kohn, m.d]. 2004321306
[neonatal tetany]. 20164857413
gamma-radiolysis of aqueous solution of proline.gamma-radiolysis of 0.05 m neutral proline solution was investigated. as the main reaction of proline radiolysis in oxygenated solutions, the hydroxylation reactions leading to hydroxyproline formation and the reactions leading to the total destruction of the pyrrolidine ring were shown. in oxygen-free solutions the yield of these reactions decreased; in this medium combination reactions giving rise to higher molecular weight combination products (up to approx. 1500) belong to the most important ...2002126589
immunopurified extracellular bartonella henselae antigen for detecting specific antibodies by enzyme immunoassay.protein antigens of bartonella henselae bacterial sonicate supernatant and concentrated cell-free culture filtrate were examined by sds-page. the sonicate supernatant gave 38 bands and the culture filtrate at least 21, of which 18 were of bacterial origin. immunoblotting against 13 monoclonal antibodies obtained from mice infected with live b. henselae showed that 10 of these antibodies reacted with a narrow 225 kda band and varying smears of bands ranging from 36 to 240 kda in the sonicate, but ...19979463512
the "mas" switch to increase efficiency of dental radiography. 1991270104
[irradiation combined with bleomycin. 5-year results with 27 o.r.l. epitheliomas (proceedings)]. 199985709
[role of electron microscopy in peripheral nerve pathology (author's transl)]. 200087154
isolation of new fastidious alpha proteobacteria and afipia felis from hospital water supplies by direct plating and amoebal co-culture procedures.as water is a source of nosocomial infections in hospitals, the presence of fastidious gram-negative bacteria in water samples taken in a university hospital was investigated. water samples were inoculated onto agar plates and into amoebal microplates for co-culture. sixty-eight alpha proteobacteria isolates were obtained and characterized using phenotypic methods and 16s rrna gene sequence comparison. the latter approach divided the strains into seven clusters. of these, one corresponded to pre ...200011102690
an experimental study on the effect of plasma expanders on blood coagulability.the effect of various plasma expanders on blood coagulation was studied using the thromboelastography (teg). solutions of 5, 10, 20, and 50% concentration of each expander (6% low molecular weight hydroxyethyl-starch (6% hespander), 3% dextran-40, 6% high molecular weight hydroxyethyl-starch (6 hes), and 6% dextran-70) or solvent (lactated ringer solution and normal saline) in blood were prepared and their coagulability was examined by teg. in the low molecular weight plasma expanders (6% hespan ...199985502
high erythrocyte membrane (na+ + k+)-atpase in kwashiorkor, in vivo reversal by diuretic.specific activity (mumol pi released/h/mg membrane protein) of ouabain-sensitive (na+ + k+)-atpase has been shown to be higher in erythrocytes from children suffering from kwashiorkor, compared to that in normal children. twenty four hours after treatment of these children with a diuretic, there was reduction in their body weights due to loss of oedema fluid. ouabain sensitive (na+ + k+)-atpase of the erythrocyte membrane was inhibited by about 40% and this was associated with gain of 1.8 mequiv ...2003150319
afipia felis induces uptake by macrophages directly into a nonendocytic compartment.afipia felis is a gram-negative bacterium that causes some cases of human cat scratch disease. a. felis can survive and multiply in several mammalian cell types, including macrophages, but the precise intracellular compartmentalization of a. felis-containing phagosomes is unknown. here, we demonstrate that, in murine macrophages, most a. felis-containing phagosomes exclude lysosomal tracer loaded into macrophage lysosomes before, as well as endocytic tracer loaded after, establishment of an infe ...200111404461
cat-scratch disease: epidemiology, aetiology and treatment.cat-scratch disease (csd) is a clinical syndrome that usually presents as a self-limiting lymphadenopathy associated with a cat scratch or bite. commonly affecting children and young adults, it has a worldwide distribution. in temperate climates, higher rates are reported in the autumn and winter, which can be attributed to the seasonal breeding of the domestic cat. the organism responsible was identified in 1983, having eluded detection for 50 years. initially, afipia felis was thought to be th ...200111440202
'metabolic' neuropathies and myopathies in infancy and childhood. 20154398416
the aquatic budding bacterium blastobacter denitrificans is a nitrogen-fixing symbiont of aeschynomene indica.blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. comparative analyses of ribosomal 16s rrna gene and internally transcribed spacer region sequences indicated that b. denitrificans is a member of the alpha-subdivision of proteobacteria. among the alpha-proteobacteria, b. denitrificans was related to a cluster of genera, including rhodopseudomonas palustris, afipia felis, nitrobacter hamburgensis, and bradyrhizobium spp. although the p ...200211872460
rapid polymerase chain reaction-based confirmation of cat scratch disease and bartonella henselae infection.cat scratch disease (csd) commonly occurs secondary to bartonella henselae infection, and the diagnosis has traditionally been made by microscopic findings, the identification of organisms by cytochemistry, and clinical history. however, cytochemical analysis tends to be very difficult to interpret, and histology alone may be insufficient to establish a definitive diagnosis of csd.200312741894
bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.a strictly aerobic, mesophilic bacterium, strain amx 51(t), was isolated from anaerobic digester sludge. cells were gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum. the isolate had phenotypic traits of the genus bosea, including cellular fatty acid and substrate utilization profiles. physiological characteristics and antibiotic susceptibility were determined. phylogenetic analysis revealed that strain amx 51(t) was a member of the alpha-proteobacteria, mo ...200313130002
rare bacterium of new genus isolated with prolonged enrichment culture.dynamic change in microbial flora was monitored with an oxygen electrode. the 1st phase microorganisms, which first grew well in lb medium, were followed by the 2nd phase microorganisms, which supposedly assimilated microbial cells of the 1st phase and their metabolites. in a similar way, a change in microbial flora was observed from the 1st phase to the 4th phase in 84 hr. based on this observation, prolonged enrichment culture was done for as long as two months to increase the ratio of existen ...200414745160
molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds.this study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some antarctic locations. pcr amplification of genes diagnostic for methylotrophs was carried out with bacterial dna isolated from 14 soil and sediment samples from ten locations on signy island, south orkney islands, antarctica. genes encoding the mxaf of methanol dehydrogenase, the fdxa for afipia ferredoxin, the msma of methanesulfonate monooxygenase, and the 16s rrna gene of methylobacteriu ...200516104352
biogenesis of afipia-containing phagosomes in non-professional phagocytes.afipia felis is a gram-negative alpha-proteobacterium, a rare cause of human cat scratch disease (csd), and likely a pathogen of amoeba. here, we show that various members of the genus afipia attach to and are taken up by various non-professional phagocytic mammalian cells (epithelial cho, endothelial ea.hy926, epithelial hela, epithelial int407 cells, endothelial hmec-1, endothelial huvec, and fibroblast l929 cells). however, only a. felis was able to do this efficiently. invasion depended on a ...200717306590
lipid microdomain-dependent macropinocytosis determines compartmentation of afipia felis.phagocytic compartments are specialized endocytic organelles and usually mature along the degradative pathway into phagolysosomes. the rare human pathogen afipia felis localizes to a compartment that is different from canonical phagocytic compartments. here, we present evidence that internalization of afipia by macrophages and unusual phagosome development are considerably decreased by attachment of cholera toxin b subunit to macrophage ganglioside gm1 or by extraction or oxidation of plasma mem ...200717319800
du nouveau sur la maladie des griffes du chat.an update on cat scratch disease: cat scratch disease is an uncommon and infectious disease of man characterized by regional lymphadenopathy following a scratch or a bite from a cat or other inoculation wounds. the role of the cat in the transmission of the disease is uncertain. this paper describes the disease, reviews different hypotheses on the etiology taking into account the recently reported discovery of a cat scratch disease bacillus and summarizes the diagnostic criteria.198617422660
idiopathic facial aseptic granuloma: a multicentre prospective study of 30 cases.idiopathic facial aseptic granuloma (ifag) was recently described in a single-centre retrospective study as a skin condition that occurs specifically in childhood.200717493068
biodegradation of haloacetic acids by bacterial isolates and enrichment cultures from drinking water systems.biodegradation is a potentially important loss process for haloacetic acids (haas), a class of chlorination byproducts, in water treatment and distribution systems, but little is known about the organisms involved (i.e., identity, substrate range, biodegradation kinetics). in this research, 10 biomass samples (i.e., tap water, distribution system biofilms, and prechlorinated granular activated carbon filters) from nine drinking water systems were used to inoculate a total of thirty enrichment cu ...200919534130
isolation and characterization of haloacetic acid-degrading afipia spp. from drinking water.haloacetic acids are a class of disinfection byproducts formed during the chlorination and chloramination of drinking water that have been linked to several human health risks. in this study, we isolated numerous strains of haloacetic acid-degrading afipia spp. from tap water, the wall of a water distribution pipe, and a granular activated carbon filter treating prechlorinated water. these afipia spp. harbored two phylogenetically distinct groups of alpha-halocarboxylic acid dehalogenase genes t ...200919634207
the afipia toolbox and its use to isolate flagellar mutants.afipia felis, a gram-negative alphaproteobacterium, has been implicated as one of the causative agents of cat scratch disease. to identify and begin to examine the virulence traits of this organism, we developed and tested a highly efficient transposon delivery system and a stable plasmid vector expressing green fluorescent protein. the transposome system is based on a tn5-derived transposon and a phage restriction endonuclease type i inhibitor. electroporation of this construct produced a libra ...201019961544
establishment of tac-negative, interleukin-2-dependent cytotoxic cell lines from large granular lymphocytes (lgl) of patients with expanded lgl populations.cell lines were established from purified large granular lymphocytes (lgl) isolated from the peripheral blood of seven patients with phenotypically homogeneous lgl expansions. lgl were stimulated with phytohemagglutinin (pha) or recombinant interleukin-2 (ril-2) and further expanded in vitro in il-2-containing media. the surface phenotype of lgl, as assessed by monoclonal antibody staining, was t3+ t8+ in five patients, t3- t8- in one, and t3+ t8- in another patient. the cells also expressed leu ...20033023432
the life cycle of ornithodoros (pavlovskyella) erraticus (acari: ixodoidea: argasidae) in the laboratory. 20133585915
[diet therapy in biliary tract diseases in children]. 1992257155
suppression of sebaceous gland activity with eicosa-5:8:11:14-tetraynoic acid. 20144225816
lower incisor extraction in orthodontic treatment.the extraction of a single mandibular incisor can be employed as a compromise treatment of certain malocclusions, if the end result fulfills the requirements for a healthier dentition which is functionally and esthetically harmonized in relation to the surrounding structures. indications, contraindications, and the procedure of treatment have been presented. class i malocclusions with normal maxillary dentition and good buccal interdigitation in which there is severe lower anterior crowding are ...1991270288
rapid expression of ornithine decarboxylase mrna in a macrophage-like cell line: camp repression of the requirement for prior protein synthesis.ornithine decarboxylase (odc) activity was rapidly induced in the raw264 macrophage-like cell line after treatment with bacterial lipopolysaccharide (lps). odc mrna levels were determined by isolating cellular rna, followed by northern blot and dot blot analysis using a 32p-labeled cdna probe. odc mrna levels increased within 1 hour of stimulation of raw264 cells with 1.0 microgram/ml lps. transcription rate analysis on isolated nuclei indicated that an increase in transcription rate contributed ...20102832424
general practitioner as general physician. 200187810
[role of natural environment in complex rehabilitation factors at health resorts]. 2002130731
traditional chinese methods of health preservation (2). 20061453764
the eeg of congenitally blind adults. 20144195664
nottingham and the hospital plan: a follow-up study of long-stay in-patients.the post-war development of psychiatric services in nottingham, with their reduction in the number of beds and marked increase in in-patient turnover and day patient and out-patient attendances, represents a close approximation to the proposals of the 1962 hospital plan. these changes were achieved by redeployment of existing resources. a fifteen-year attrition study of 1960 long-stay in-patients shows a high rate of discharge, especially for males. the reasons for these findings and some implic ...2004497624
[changes in energy metabolism body composition and hormone adaptation in childhood protein calorie malnutrition]. 1998104898
prevalence of antibodies to bartonella henselae in patients with suspected cat scratch disease (csd) in italy.cat scratch disease (csd) is a relatively new diagnosed illness with clinical signs of self-limiting regional lymphadenopathy accompanied by symptoms of fever and malaise, to encephalopathy and neuropathy, occurring after a cat scratch or flea bite. bartonella henselae is now accepted as the etiologic agent of csd. from january 1994 to september 1998, 412 patients were evaluated for suspect csd in italy. sera were tested for antibodies to b. henselae by a commercially available indirect immunofl ...199910485354
techniques for the study of polypeptide hormone inactivation at receptor sites. 2003165371
[highly hydrophilic soft lenses. optic indications for permanent wearing and adaptation technic].after the indications of the various contact prostheses are briefly reviewed, the interest of continuous wearing of optical hydrophilic soft lenses is emphasized. aphakia, high ametropis and anisometropia are the main indications. yet, continous wearing should be recommended only to patients who have difficulty with contact lens manipulation. the patient will be admitted twice in hospital for the fitting and cared for twice a day which permits to get over the difficulty of the first two nights. ...2002130866
direct in vivo tendon force measurement system. 20082292606
freedom or control? 20164793742
wilfred ruprecht bion. 200193225
isolation and properties of methanesulfonate-degrading afipia felis from antarctica and comparison with other strains of a. felis.three novel strains of methylotrophic afipia felis were isolated from several locations on signy island, antarctica, and a fourth from estuary sediment from the river douro, portugal. they were identified as strains of the alpha-2 proteobacterium a. felis by 16s rrna gene sequence analysis. two strains tested were shown to contain the fdxa gene, diagnostic for a. felis. all strains grew with methanesulfonate (and two strains with dimethylsulfone) as sole carbon substrate. growth on methanesulfon ...200515643932
[left ventricular function of the coronary patient: relation between ventricular kinetic disorders and alterations of myocardial contractility].the left ventricular kinaetics of 29 coronary patients (pure angina and with sequela of myocardial infarction) was studies by biplane angiocardiography. their contractility was assessed by measurement of the vmax and vecmax indices derived from the relationship between contractile elements shortening speed-overall wall tension, in isovolumetric phase. an excellent relationship links the hypokinaetic area with decrease of the ejection fraction (sv/lsv): when the hypokinaetic area exceeded 20% of ...2002123738
minoxidil in resistant hypertension.minoxidil in combination with propranolol and diuretics controlled the blood-pressure in a group of hypertensive patients who were resistant to treatment with large doses of standard drugs. the main problem was fluid retention but subjective side-effects were fewer than in a comparable group on other drugs.199995728
overdenture therapy: a longitudinal report. 20143905669
echo-encephalographic measurements (a-scan) of the lateral ventricles in children; its reliability.a positioning of the probe on the skull has been developed to increase the diagnostic accuracy of hydrocephalus. echo-encephalography in the trigonum region of the ventricles is usually possible in children up to 13 years of age. in this region sources of error as the medial wall of the temporal lobe or the lateral wall of the temporal horn are eliminated. in hydrocephalic ventricles the ultrasound measurements in this region corroborate very well with the measurements found in the pneumo-enceph ...1994201414
calcium does not inhibit active sliding of microtubules from mussel gill cilia. 2003154066
effectiveness of benzathine penicillin regimen in the treatment of syphilis in pregnancy.the intramuscular injection of benzathine penicillin g 2.4 million units weekly for 3 consecutive weeks to syphilitic pregnant women was again confirmed to be clinically effective for prevention of their neonates from congenital syphilis and well accepted as treatment for syphilis in pregnancy. it was administered to 184 cases, or 93.5 per cent, of 197 syphilitic gravidas. it was found that pregnancy outcomes in terms of abortion, stillbirth, prematurity, full term delivery, neonatal birth weigh ...20022738484
nephrotic syndrome in patient on captopril. 199988638
[cost and benefit study of different preventive systems applicable to the spanish child population. v. accidents and complications of minor oral surgery]. 2003149550
the diagnosis and management of sudden collapse in dental practice. part 3. collapse in the dental chair under local anaesthesia. 1991270354
effects of amino acid substitutions outside an antigenic site on protein binding to monoclonal antibodies of predetermined specificity obtained by peptide immunization: demonstration with region 113-120 (antigenic site 4) of myoglobin.immunochemical cross-reactivity of protein variants has been very frequently used to map protein antigenic sites. the approach is based on the assumption that amino acid substitutions affecting the binding of a protein to its antibody, particularly when monoclonal antibodies (mabs) are used, must be part of the antigenic site and not far from it. the assumption was investigated in this study by determining the effects of amino acid substitutions outside the antigenic site on the reactivity of si ...20011281636
identification of a novel afipia species isolated from an indian flying fox.an old world fruit bat pteropus giganteus, held in captivity and suffering from necrosis of its wing digits, failed to respond to antibiotic therapy and succumbed to the infection. samples submitted to the national centre for foreign animal disease were tested for viral infection. vero e6 cells exhibited minor but unique cytopathic effects on second blind passage, and full cpe by passage four. utilizing an unbiased random amplification technique from cell culture supernatant, we identified a bac ...201525874801
experimental infection of cats with afipia felis and various bartonella species or subspecies.based upon prior studies, domestic cats have been shown to be the natural reservoir for bartonella henselae, bartonella clarridgeiae and bartonella koehlerae. however, other bartonella species, such as bartonella vinsonii subsp. berkhoffii, bartonella quintana or bartonella bovis (ex weissii) have been either isolated from or bartonella dna sequences pcr amplified and sequenced. in the late 1980s, before b. henselae was confirmed as the etiological agent of cat scratch disease, afipia felis had ...201424972870
[distribution of potential pathogenic bacteria in the jiulong river watershed].recently, the human activities including economic growth and urbanization posed serious environmental health risks to the jiulong river watershed (jrw). in order to gain a full understanding of the distribution of potential pathogenic bacteria (ppb) in this area, we used 16s rrna amplicon pyrosequencing technology to investigate planktonic and benthic bacterial community in two main tributaries (north river, nr, and west river, wr) of the jiulong river (jr). at the genus level, a total of 68 gen ...201425055661
highly sensitive bacteriophage-based detection of brucella abortus in mixed culture and spiked blood.for decades, bacteriophages (phages) have been used for brucella species identification in the diagnosis and epidemiology of brucellosis. traditional brucella phage typing is a multi-day procedure including the isolation of a pure culture, a step that can take up to three weeks. in this study, we focused on the use of brucellaphages for sensitive detection of the pathogen in clinical and other complex samples, and developed an indirect method of brucella detection using real-time quantitative pc ...201728604602
how to encourage a child to drink extra fluids. 199985295
[otorhinolaryngologic surgery in patients with coagulopathy].a personal experience of orl surgery in patients suffering from bleeding disorders is presented. 26 patients have been submitted to different surgical procedures, fibrin glue being used in all of them. treatment results were as satisfactory as those in normal patients.20012631911
smoking habits of 611 patients with oral lichen planus.the smoking habits of 611 patients (68 per cent females and 32 per cent males) with oral lichen planus were studied. forty-six per cent were daily smokers, 4 per cent smoked only at social events, and 50 per cent were nonsmokers. in comparison with the nonsmokers, the daily smokers showed significantly lower prevalences of reticular and atrophic types of oral lichen planus lesions and a significantly higher prevelance of the plaque type (p less than 0.001). it is suggested that these findings de ...1992265045
cardiac tamponade and peripheral eosinophilia in a patient receiving cromolyn sodium. 200095924
[thrombocytopenias. radioisotope studies. platelet survival studies]. 2002120271
genome sequence of afipia felis strain 76713, isolated in hospital water using an amoeba co-culture procedure.afipia felis is a gram-negative alphaproteobacterium originally described as the agent of cat-scratch disease (csd). we sequenced the genome from a strain of a. felis, which was recovered from a hospital water sample using an amoebal co-culture procedure. it is composed of 3,989,646 bp, with a g+c content of 61.27% and encodes 4,068 protein-coding genes and 53 rna genes.201425540358
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