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microbial etiology of periodontal diseases. where are we? where are we going?many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ...19921325846
e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ...19921324301
comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms.the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ...19921331019
subgingival temperature (iii). relation to microbial counts.the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ...19921321846
inactivation of human serum bactericidal activity by a trypsinlike protease isolated from porphyromonas gingivalis.a protease was isolated from an outer membrane vesicle preparation of porphyromonas gingivalis atcc 33277 and assessed for its ability to inactivate the bactericidal activity of normal human serum. the enzyme, which was activated by reducing agents, was found to be a trypsinlike protease with a molecular mass of approximately 80 kda. prior to being tested in the bactericidal assay, pooled human serum was preincubated with the partially purified enzyme. under conditions in which the trypsinlike p ...19921314221
the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction.the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ...19921318331
immunoglobulin-degrading enzymes in localized juvenile periodontitis.previous reports have indicated the association of periodontal diseases with elevated levels of serum immunoglobulin g (igg) antibodies to periodontally relevant bacteria. recent results from this laboratory suggest that enzymes proteolytic for immunoglobulins are important virulence factors of several periodontal bacteria. specifically, enzymes from porphyromonas (bacteroides) gingivalis culture supernatant fluid (sf) cleaved human igg (4 subclasses), iga1 and iga2, igm, igd and ige. proteolyti ...19921318974
microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics.a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ...19921315389
periodontal bone loss in mice induced by different periodontopathic organisms.periodontal bone loss in mice orally inoculated with peptostreptococcus anaerobius, pept. magnus and actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice. six-to-8-week-old balb/c mice were inoculated with 1 x 10(5), 1 x 10(7) or 1 x 10(9) colony-forming units (c.f.u.) of bacteria in 50 microliters of medium. ten mice received each concentration of bacteria and 10 sham-inoculated mice acted as controls. five mice from each of the groups were killed 6 weeks after inoc ...19921353347
immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium.immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ...19921354674
the effect of 3 mouthrinses on plaque and gingivitis development.the aim of this study was to evaluate the effect of 3 mouthrinses, listerine antiseptic (thymol), peridex (chlorhexidine), perimed (povidone iodine and hydrogen peroxide), and a placebo (water) on the development of dental plaque and gingivitis, when used as the only oral hygiene procedure for 14 days. 71 subjects were entered into a randomized, double-blind study. at the baseline examination, papillary bleeding score (pbs), and plaque index (pi) were registered, after which subjects received su ...19921310096
synonymy of strains of center for disease control group df-1 with species of capnocytophaga.of eight strains of center for disease control group df-1 examined, seven had 62 to 87% deoxyribonucleic acid homology with the neotype strain of capnocytophaga ochracea and one had 72% deoxyribonucleic acid homology with the type strain of c. gingivalis. deoxyribonucleic acid homology of four strains of bacteroides ochraceus with the neotype strain of c. ochrecea was 76 to 86%.1979528685
putative periodontal pathogens on titanium implants and teeth in experimental gingivitis and periodontitis in beagle dogs.the microflora around titanium implants and teeth in 4 beagle dogs was analyzed in order to follow the longitudinal development from healthy conditions to experimental gingivitis and periodontitis. a 2-month plaque control program was performed to establish healthy conditions on titanium implants and control teeth (baseline, day 0). subgingival bacterial samples and radiographs were obtained. the plaque control was ceased and all measurements were repeated on day 21 (gingivitis). a new period of ...19921290791
nucleotide sequences of 16s rrna encoding genes from capnocytophaga ochracea atcc 33596, capnocytophaga sputigena atcc 33612 and capnocytophaga gingivalis atcc 33624. 19921280812
detection, identification, and comparison of capnocytophaga, bacteroides ochraceus, and df-1.working independently, three laboratories had recognized considerable similarity among certain strains of dysgonic, fermentative, capnophilic, surface translocating, gram-negative bacilli referred to as capnocytophaga, bacteroides ochraceus, and center for disease control biogroup df-1. to determine the relationship among these groups, 21 strains were exchanged and independently characterized by the three laboratories. additionally, a fourth laboratory examined the deoxyribonucleic acid homologi ...1979528686
ultrastructure of gliding bacteria: scanning electron microscopy of capnocytophaga sputigena, capnocytophaga gingivalis, and capnocytophaga ochracea.when examined by both light and scanning electron microscopy, capnocytophaga gingivalis, c. sputigena, and c. ochracea displayed three distinct growth zones: the original streak, an intermediate zone, and the advancing edge, or halo zone. on trypticase (bbl microbiology systems)-soy-blood agar, the cells translocated by gliding. c. gingivalis and c. sputigena formed large, irregular isolated colonies, while c. ochracea formed a more confluent cell mass. the cells within the streak zone and in mo ...1979528052
capnocytophaga: new genus of gram-negative gliding bacteria. i. general characteristics, taxonomic considerations and significance.the characteristics of gliding bacteria isolated from both healthy and diseased sites in the oral cavity are, summarized and the taxonomic position of the bacteria discussed. uniform attirubtes of the fusiform isolates include gliding motility, strictly fermentative metabolism dependent on the presence of co2 (or hco3-), under either anaerobic or aerobic conditions, presence of benzidine-reactive components, and the production of acetic and succinic acids as the major or sole, acidic, metabolic ...1979518239
capnocytophaga: new genus of gram-negative gliding bacteria. iii. physiological characterization.sixty-eight strains of capnophilic fusiform gram-negative rods from the human oral cavity were subjected to extensive physiologic characterization, tested for susceptibility to various antibiotics, and the mol-percent guanine plus cytosine of each isolate determined. the characteristics of the isolates were compared with 10 fresh and 2 stock isolates of fusobacterium nucleatum. the isolates clearly differed from the fusobacterium species on the basis of mol-percent guanine plus cytosine, end pro ...1979518236
fatty acid composition of gliding bacteria: oral isolates of capnocytophaga compared with sporocytophaga.the extractable and bound lipids and cellular fatty acids of the gram-negative gliding bacteria, capnocytophaga sputigena, c. gingivalis, and c. ochracea were compared to the non-host-related gliding bacterium sporocytophaga myxococcoides. the extractable lipids represented between 17 and 28% of the cell dry weight, whereas only 2 to 4% of the lipids were in the bound fraction. the methyl esters of the cellular fatty acids were mainly ac15:0, which accounted for 69 to 73% of the total extractabl ...1979500207
phospholipid composition of gliding bacteria: oral isolates of capnocytophaga compared with sporocytophaga.the distribution of acetone-soluble (neutral glycolipid) and acetone-insoluble (phospholipid isoprenoids) lipids in oral isolates of gram-negative gliding bacteria of the genus capnocytophaga was compared with those in a non-host-related gliding bacterium, sporocytophaga myxococcoides. the acetone-soluble material accounted for 34 to 55% of the extracted lipids; the remainder was acetone-insoluble material. the major phospholipid was phosphatidylethanolamine (67%), with lesser amounts of lysopho ...1979500209
a neutrophil disorder induced by capnocytophaga, a dental micro-organism.we recovered capnocytophaga, a gram-negative anaerobe implicated in the pathogenesis of periodontal disease, from two patients with a history of dental infections. neutrophils from both patients failed to acquire the asymmetric shape characteristic of normal neutrophils. fluorescein staining of the patients' living neutrophils remained diffuse and patchy instead of showing the normal pattern in which the fluorescence is swept into the rear of the cell. the locomotion of one patient's neutrophils ...1979481526
susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase.the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ...19921481764
cytochromes and dehydrogenases in membranes of a new human periodontal bacterial pathogen, capnocytophaga ochracea. 1979231958
septicemia due to capnocytophaga (bacteroides ocharaceus) in hodgkin's disease. 1979434659
periodontal therapy in humans. i. microbiological and clinical effects of a single course of periodontal scaling and root planing, and of adjunctive tetracycline therapy.the present results showed that maarked and long-lasting changes in the subgingival microflora associated with periodontal disease could be achieved by a single course of periodontal treatment. immediately following therapy, the total number of subgingival organisms decreased 10- to 100-fold and the proportions of cultivable gram negative organisms and anaerobic organisms generally decreased 3- to 4-fold or more. after treatment, most periodontal pockets were populated by a scant microflora pre ...1979385821
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
capnocytophaga canimorus infection and acute renal failure. 19921522317
[aquatic myxobacteria (sporocytophaga cauliformis) and the order "myxobacterales" (author's transl)].the indicator function of aquatic myxobacteria for the purpose of evaluating drinking water quality as well as their occurrence in sewage effluent make it desirable to describe these hitherto little known organisms in more detail. to this end, a comparative investigation of anaerobic myxobacteria of the genus sphaerocytophaga, two typical representatives of the order myxobacterales (myxococcus fulvus, sporocytophage cauliformis), and a strain ov vitrepscilla (vitroescilla proteolytica) was under ...1979120650
enumeration of subgingival species on primary isolation plates using colony lifts.this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ...19921528619
group and type antigens of capnocytophaga.group-specific and type-specific antigens have been identified and purified from sonic extracts of capnocytophaga. the group-specific antigen, which was purified by affinity chromatography, was found to be identical to an antigen present in all 26 strains tested. the antigen is sensitive to trypsin, sodium dodecyl sulfate and heat labile, and composed predominantly (55%) of protein. the type-specific antigen, which was obtained by preparative immunoelectrophoresis, was found to be present in onl ...1979106014
the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...197944923
putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene.the aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural kenyan population exhibiting poor oral hygiene and widespread loss of attachment (la). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with la greater than or equal to 4 mm; at least 5 mm la and a pocket greater than or equal to 4 mm interproximally ...19921346265
a sensitive enzymatic method (sk-013) for detection of treponema denticola, porphyromonas gingivals and bacteroides forsythus in subgingival plaque samples.an enzymatic method, sk-013, was developed for rapid detection of the peptidase activity in subgingival plaque samples. this method was found to have specificity for porphyromonas gingivalis, treponema denticola, bacteroides forsythus, and some capnocytophaga strains. the purpose of this study was to determine whether sk-013 could indicate the presence of periodontopathic bacteria, including t. denticola, p. gingivalis and b. forsythus, which produce trypsin-like enzymes. subgingival plaque samp ...19921312592
role of porphyromonas gingivalis 40-kda outer membrane protein in the aggregation of p. gingivalis vesicles and actinomyces viscosus.porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with actinomyces viscosus cells. a 40-kda outer membrane protein (omp)-coding gene from p. gingivalis was cloned and the protein was found to be localized in these vesicles. the recombinant 40-kda omp did not show aggregation activity. however, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of p. gingivalis vesicles with a. viscosus cell ...19921329700
a sensitive enzymatic method (sk-013) for detection and quantification of specific periodontopathogens.porphyromonas gingivalis, bacteroides forsythus, and treponema denticola have been found to predominate in periodontal pockets of patients with adult periodontitis. these microorganisms hydrolyze the synthetic peptide n-benzoyl-dl-arginine-2-naphthylamide (bana). in this study, we developed an enzymatic method, designated sk-013, to detect the existence of these microorganisms in subgingival plaque bacteria. this enzymatic method was based on the observation of the hydrolysis of n-carbobenzoxy-g ...19921312591
opportunistic capnocytophaga canimorsus infection. 19921346312
human neutrophil azurocidin synergizes with leukocyte elastase and cathepsin g in the killing of capnocytophaga sputigena.azurocidin was purified in the presence of phenylmethylsulfonyl fluoride. electrophoresis revealed at least seven species which exhibited n-terminal sequences consistent with azurocidin. azurocidin exhibited no bactericidal activity against capnocytophaga sputigena or other oral bacteria but synergized the bactericidal activity of enzymatically active elastase. azurocidin also interacted synergistically with cathepsin g.19921399008
modulation of human neutrophil adherence by oral bacteria.polymorphonuclear neutrophils (pmns) comprise over 90 per cent of leukocytes in the oral cavity. although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. adherence of pmns to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. in this study, oral bact ...19921605750
isolation and characterization of enzymes hydrolyzing chymotrypsin synthetic substrate (enzyme i) and trypsin synthetic substrate (enzyme ii) from the envelope of capnocytophaga gingivalis.enzymes hydrolyzing chymotrypsin synthetic substrate and trypsin synthetic substrate, referred to as enzyme i and enzyme ii, respectively, were found in the envelope fraction of capnocytophaga gingivalis (atcc 33624). detergent extraction of both enzymes were purified by gel filtration, ion exchange chromatography, and affinity chromatography. the enzyme i was a serine-containing metallo enzyme with a molecular mass of 77 kda. the molecular mass of the enzyme ii was 83 kda, and it was inhibited ...19921406457
actinobacillus actinomycetemcomitans, capnocytophaga and porphyromonas gingivalis in subgingival plaque of adolescents with down's syndrome.levels of actinobacillus actinomycetemcomitans, capnocytophaga and porphyromonas gingivalis were determined in subgingival plaque samples from 37 adolescents with down's syndrome and 37 healthy controls matched with respect to age and sex. gingival inflammation, supra- and subgingival calculus, periodontal pockets ( > 4 mm) and alveolar bone loss were registered. alveolar bone loss was more frequent in down's syndrome subjects (32%) than in the controls (3%). a. actinomycetemcomitans was detecte ...19921408360
effect of extraction of partly erupted third molars on subgingival microorganisms.this study was made to investigate the effect of extraction of third molars on subgingival microbes in 39 generally and gingivally healthy men with an average age of 20.2 years (sd 0.9). microbial samples were taken from the pericoronal space of symptom-free partly erupted lower third molars and from the adjacent gingival pockets of the second molars. the samples were cultivated anaerobically. all partly erupted third molars were extracted from 20 subjects. a control group of 19 subjects was lef ...19921408014
relation of baseline microbial parameters to future periodontal attachment loss.the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...19911661304
[meningitis caused by capnocytophaga carnimorsus with development of erythema nodosum].a case of meningitis in a 42-year-old previously healthy man caused by the gram-negative rod capnocytophaga carnimorsus is presented. the patient had erythema nodosum. bacteremia was possibly induced during dental treatment.19921413165
serum antibody responses in human periodontitis to cellular components of capnocytophaga.serum levels of igm, igg and igg-antibody subclasses directed against cell envelopes, lipopolysaccharides and cytoplasmic fractions from capnocytophaga sputigena, c. gingivalis and c. ochracea were examined in age-, race- and sex-matched periodontally healthy (n = 25) subjects and subjects with adult periodontitis (n = 25). the envelopes and cytoplasmic fractions were obtained by ballistic disintegration of the cells and ultracentrifugation. cell envelopes were treated with dnase, rnase and lyso ...19921417521
capnocytophaga: new genus of gram-negative gliding bacteria. ii. morphology and ultrastructure.gram-negative, anaerobic gliding bacteria were isolated from normal supragingival plaque and from periodontal lesions. isolates could be divided into two size classes: small 2.4-4.2 micrograms x 0.38-0.5 microgram and large 4.8-5.8 micrograms x 0.42-0.6 microgram cells. the outer membrane was either loose-fitting and wavy, or taut, and of variable thickness. an electron-dense fuzz was discernible on several of the isolates. the periplasmic region was of variable electron-density. the genus capno ...1979518235
antimicrobial susceptibilities and beta-lactamase characterization of capnocytophaga species.capnocytophaga species have been associated with a wide variety of infections in both immunocompetent and immunocompromised patients. on the basis of data from antimicrobial susceptibility studies, beta-lactam antibiotics have been considered efficacious therapy. six of 19 isolates from primarily clinical sources across canada demonstrated beta-lactamase production, and agar dilution susceptibility testing showed broad resistance to beta-lactam antibiotics. for the beta-lactamase producing isola ...19921444299
surgical treatment of facial dog bite injuries in children. 19921464687
capnocytophaga canimorsus septicemia associated with cat scratch.a case is reported of capnocytophaga canimorsus (formerly cdc group df-2) septicemia following cat scratch in a patient with congenital asplenia. the case is of interest in that (1) the clinical presentation provided no indication as to the cause of the patient's illness. (2) c. canimorsus infection following cat bite or scratch has been reported far less frequently than following dog bite. (3) the clinical course of the illness may have been modified by previous and concurrent warfarin therapy.19921437293
relationship between oral gram-negative anaerobic bacteria in saliva of the mother and the colonization of her edentulous infant.various gram-negative anaerobic bacterial species can be detected in the oral flora of edentulous infants. however, knowledge of the initial infection source is still scarce. thirty caucasian mothers (mean age 30 years) and their edentulous infants (mean age 3 months) were examined for the possible similarity of the oral gram-negative anaerobic flora. paraffin-stimulated saliva was collected from the mothers. a pooled swab sample from mucosal surfaces and unstimulated saliva were collected from ...19921494450
infective endocarditis with involvement of the tricuspid valve due to capnocytophaga canimorsus.a case of endocarditis with vegetations on the tricuspid valve caused by capnocytophaga canimorsus is described. extensive diagnostic investigations preceded the diagnosis, including blood cultures, 34 of which were sterile. a possible role of the pulmonary circulation in the negative blood cultures is discussed.19921468422
antibiotic-impregnated methylmethacrylate in treatment of infections with spinal instrumentation. case report and technical note. 19921523501
the early colonization of gram-negative anaerobic bacteria in edentulous infants.the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ...19921528621
microbiota associated with residual clefts and neighboring teeth in patients with cleft lip, alveolus, and palate.twenty patients with residual clefts or pronounced soft tissue grooves, treated for uni- or bilateral cleft lip, alveolus, and palate were included in this study. ten patients were recalled for dental prophylaxis at regular intervals, 10 patients were not. one microbiologic sample was obtained from the cleft area and two samples from a tooth adjacent to the cleft (sites adjacent and distant to the cleft). between the recall and the nonrecall group there were notable differences in the presence o ...19921472527
isolation of cdc group m-5 and staphylococcus intermedius from infected dog bites. 19921474272
pleural infection caused by capnocytophaga canimorsus, formerly cdc group df-2.an 80-year-old man with waldenström's macroglobulinemia developed pneumonia and empyema due to capnocytophaga canimorsus (formerly cdc group df-2). no growth was detected in blood cultures, but the organism was identified from cultures of pleural fluid. the infection responded well to antibiotics and drainage via a chest tube. to our knowledge, this is the first report of a clinical isolate of c. canimorsus from a pulmonary source. the absence of concurrent bacteremia raises the possibility that ...19921520767
capnocytophaga: new genus of gram-negative gliding bacteria. iv. dna base composition and sequence homology. 1979117782
bite wounds and infection.one of every two americans will be bitten by an animal or by another person at some point. bites account for approximately 1% of all visits to emergency rooms; injuries inflicted by dogs are most common. the bacteria involved in infection of bite wounds include pasteurella multocida, staphylococcus aureus, staphylococcus intermedius, alpha-hemolytic streptococci, capnocytophaga canimorsus, and other members of the oral flora. anaerobic bacteria are present in approximately one-third of bite woun ...19921562653
capnocytophaga bacteremia in a patient with hodgkin's disease following bone marrow transplantation: case report and review.capnocytophaga is a gram-negative, capnophilic, facultatively anaerobic bacillus that normally inhabits the oral cavity. we report the case of a patient who developed capnocytophaga bacteremia following autologous bone marrow transplantation for hodgkin's disease, and we review other reported cases of capnocytophaga bacteremia in immunocompromised patients. in our case infection followed pretransplantation conditioning and was associated with severe oral mucositis and neutropenia. antibiotic the ...19921600004
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
dog-bite induced capnocytophaga canimorsus septicaemia. 19921580872
capnocytophaga canimorsus endocarditis.we report a case of aortic valve endocarditis, caused by capnocytophaga canimorsus (previously known as cdc group df-2) secondary to a dog bite. vegetations were documented by cross-sectional echocardiography and bacteraemia by positive haemocultures for this unusual gram-negative bacillus.19921577022
[polymorphonuclear leukocyte-mediated effects on human oral keratinocytes by periodontopathic bacterial extracts].in order to investigate the polymorphonuclear leukocyte-mediated oral keratinocyte injury, the author established a method to culture keratinocytes (scc9 and gingival keratinocytes) and subjected to the cell detachment assay by polymorphonuclear leukocytes and/or bacterial ultrasonic extracts from actinobacillus actinomycetemcomitans y4, bacteroides forsythus omz 408, fusobacterium nucleatum atcc 10953, capnocytophaga sputigena 4 and eikenella corrodens 1073. the bacterial extracts alone caused ...19921607829
microbial risk indicators for periodontal attachment loss. 19911831856
[capnocytophaga sepsis in a man with acute lymphoblastic leukemia]. 19921630194
early effects of periodontal therapy on the detection frequency of four putative periodontal pathogens in adults.this study aimed to examine early posttreatment changes in the periodontal microflora. paper point sampling and conventional bacterial cultivation were used to monitor the effects of surgical and non-surgical periodontal therapy on the detection frequency of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and capnocytophaga species in deep periodontal pockets. ten patients, 5 men and 5 women (mean age 44 years), with advanced periodontal disease were select ...19921625154
identification by biotinylated dna probes of capnocytophaga species isolated from supragingival calculus.the distribution, genetic relatedness, and peptidase activity in hydrolyzing n-benzoyl-l-arginine-p-nitroanilide by capnocytophaga species isolated from calculus- and non-calculus-formers were determined in this study. the proportion and prevalence of capnocytophaga species were higher in plaque samples from the calculus group than in those from the non-calculus group, and these bacteria showed high peptidase activity. c. gingivalis were found more frequently in the calculus group than in the no ...19911648580
clinical and microbiological patterns of adults with periodontitis.although current data suggest that periodontitis may actually be multiple diseases each with varying responses to therapy, little evidence exists to support this theory in adult patients. this report describes the design and initial findings of a longitudinal field trial involving the diagnosis and treatment of adult and refractory periodontitis patients in private practice. adult patients (n = 221) who met specific clinical criteria were selected from the private practices of 22 periodontists. ...19911663156
fulminant capnocytophaga canimorsus (df-2) septicaemia. 19911671152
fatal septicemia with capnocytophaga canimorsus in a compromised host. a case report with review of the literature.capnocytophaga canimorsus (df-2) is a newly described gram-negative bacterium that can produce serious infections following dog bites in the immunocompromised host. we are reporting a case of c. canimorsus infection in an elderly patient with a history of chronic respiratory disease and alcohol abuse. we also give a review of the clinical spectrum of the infection, discuss the difficulty in establishing the diagnosis and the preferential treatment.19911665939
periodontal disease in hiv-infected patients in relation to lymphocyte subsets and specific micro-organisms.visible plaque index (vpi), gingival bleeding index (gbi) and pocket depth (pd) were analyzed in relation to potential periodontal pathogenic microorganisms and peripheral numbers of t4+ and t8+ lymphocyte subsets in 10 patients with human immunodeficiency virus (hiv) infection, 10 patients with aids related complex (arc) and 10 patients with acquired immune deficiency syndrome (aids). 10 healthy persons served as controls. periodontal disease in patients with more advanced stages of hiv infecti ...19911677365
capnocytophaga canimorsus infection after dog-bite. 19911672932
[bacteriological study on juvenile periodontitis].the predominant cultivable microflora of 23 pockets in 15 juvenile periodontitis (jp) patients was studied for the first time in china using the current anaerobic methodology. samples were taken with sterile paper points and dispersed on a vortex mixer. then the diluted samples were plated on the non-selective blood agar plates and selective mgb medium which favors the growth of actinobacillus actimycetemcomitans (aa) and incubated in anaerobic chamber for 5 days. from each sample 15 or more iso ...19911676335
isolation and identification of capnocytophaga canimorsus (df-2) from blood culture. 19911677720
[the study of major anaerobic bacteria from subgingival plaques of juvenile periodontitis].the studies of the subgingival plaques from juvenile periodontitis (jp) have shown that jp is associated with haemophilus actinomycetemcomitans (h. a), capnocytophaga (capno.) and other species. this study was designed to study these species with chinese jp patients using selective cultivable technique. the media used include tsbv to support h. a, tbbp to support capno. and selective media for bacteroides gingivalis. a total of 303 subgingival samples were collected from 43 jp, 31 gingivitis and ...19911687730
the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid.in this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ...19901696949
isolation and properties of the capnocytophaga ochracea bacteriocin.a bacteriocin from a subgingival plaque isolate of capnocytophaga ochracea was purified and characterized. it was isolated from cell extract and had a molecular weight of 100,000. streptococcus sanguis, streptococcus mitis, streptococcus mutans and propionibacterium acnes were susceptible to the bacteriocin.19921528638
immunoglobulin a1 (iga1) proteases from prevotella (bacteroides) and capnocytophaga species in relation to periodontal diseases. 19911715399
microbial aspects of failing osseointegrated dental implants in humans.the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ...19911906537
capnocytophaga canimorsus septicemia caused by a dog bite in a hairy cell leukemia patient.fatal septicemia developed in a splenectomized patient with hairy cell leukemia following a dog bite. capnocytophaga canimorsus, a slowly growing gram-negative bacillus, was isolated from the patient's blood. although a rare complication of dog bites in the normal population, this bacterium should be suspected and promptly treated in immunologically compromised dog bite victims. furthermore, immunocompromised patients should be made aware of the dangers of dog ownership.19921734053
biochemical comparison of proteolytic enzymes present in rough- and smooth-surfaced capnocytophagas isolated from the subgingival plaque of periodontitis patients.four rough-surfaced (r) and three smooth-surfaced (s) clinical isolates of capnocytophaga obtained from the subgingival plaque of periodontitis patients were studied for their peptidase and protease profiles. the results were compared with those obtained with c. gingivalis (which has a smooth morphology). all cell extracts obtained by ultrasonic treatment displayed high peptidase activity toward n-aminoacyl-2-naphthylamines, the best substrates being the arginyl, aspartyl, and leucyl derivatives ...19911825330
minocycline slow-release formulation effect on subgingival bacteria.the purpose of this study was to determine the microbiological efficacy of an adjunctive minocycline periodontal formulation delivered subgingivally. subjects were systemically healthy but exhibited severe periodontitis; i.e., probing depths greater than 6 mm. the two study groups included individuals who received minocycline or a placebo periodontal formulation after root planing. subgingival plaque samples were obtained at baseline; prior to treatment; and at 1, 3, and 6 months. plaque was eva ...19921552468
[perspective: new pathogens: branhamella catarrhalis, capnocytophaga turleri, corynebacterium group jk].branhamella catarrhalis is an important pathogen of upper and lower respiratory tract. according to findings in literature, it mostly causes bronchopulmonary infections. capnocytophaga is a newly recognized genus of the gram negative bacilli. in various reports, it has been recognized that capnocytophaga mostly causes sepsis. bacteria of corynebacterium group jk (cjk) cause many important local infections and sepsis.19911795663
severe intrauterine infection due to capnocytophaga ochracea.a case of severe neonatal sepsis due to intrauterine infection with capnocytophaga ochracea, complicated by hypotension, renal failure and disseminated intravascular coagulation, is reported. the role of capnocytophaga as an organism of increasing importance in intrauterine and peripartum infections is discussed.19911797120
[capnocytophaga meningitis in a non immunocompromised patient]. 19911828587
effects of anaerobiosis and aerobiosis on interactions of human polymorphonuclear leukocytes with the dental plaque bacteria streptococcus mutans, capnocytophaga ochracea, and bacteroides gingivalis.human polymorphonuclear leukocytes (pmn) were able to generate and release superoxide anions upon stimulation of streptococcus mutans, bacteroides gingivalis, and capnocytophaga ochracea when incubated aerobically but not when incubated anaerobically. lysozyme release and phagocytosis by pmn were independent of oxygen, and no difference between pmn incubated aerobically or anaerobically was observed (pmn stimulated by b. gingivalis released 7.6% total lysozyme when aerobic and 6.9% when anaerobi ...19911847699
bacteriological study of juvenile periodontitis in china.the predominant cultivable bacteria associated with juvenile periodontitis (jp) in china were studied for the first time. subgingival plaque samples were taken on paper points from 23 diseased sites in 15 jp patients and from 7 healthy sites in 7 control subjects. serially diluted plaque samples were plated on nonselective blood agar and on mgb agar, a selective medium for the isolation of actinobacillus actinomycetemcomitans. fifteen or more isolated colonies from each sample (in sequence witho ...19911832453
microbiological and immunological aspects of experimental periodontal disease in rats: a review article.animal models in which microbiological and immunological aspects of periodontal disease can be studied prospectively seem well warranted. the rat bears much resemblance to man with respect to periodontal anatomy, development and composition of dental plaque, histopathology of periodontal lesions, and basic immunobiology. furthermore, reproducible methods are available for assessment of periodontal disease in rats, and detectable periodontal destruction can be induced in a few weeks in these anim ...19912002433
use of synthetic oligonucleotide dna probes for the identification of different strains of fusobacterium nucleatum.the ability of three different oligonucleotide probes to identify different oral strains of fusobacterium nucleatum was tested. probes 119 and 214 were designed on the basis of known amino acid sequences from the n-terminal end of an outer membrane protein of f. nucleatum strain fev1. probe h 2.1, was a randomly cloned chromosomal dna fragment of 2.1 kbp. the specificities of the probes were examined by testing each of them against a panel of five other species of fusobacterium (six strains), or ...19911837056
staining and antimicrobial properties in vitro of some chlorhexidine formulations.dietary staining studies have proved useful determinants of chlorhixidine activity in mouthrinse products, and results correlate with plaque inhibitory effects. this investigation compared the staining and antimicrobial action in vitro of two known and similarly effective, commercially available chlorhexidine mouthrinses with a reformulated 0.1% chlordexidine preparation. after adjustment for original concentration the 0.2%, 0.12% and reformulated 0.1% products had essentially similar, minimum i ...19911860282
secretory diarrhea following a dog bite.overwhelming sepsis is rarely complicated by secretory diarrhea. we present a case of fulminant sepsis associated with dermatologic manifestations, disseminated intravascular coagulation (dic), and a severe secretory diarrhea that has not previously been described. this followed a dog bite, and the patient was otherwise healthy. the combination of septic shock, skin lesions, and dic occurring after a dog bite has been attributed to dysgonic fermentor 2 organisms, now called capnocytophaga canimo ...19911864208
application of quadferm+ for the identification of fastidious gram-positive and gram-negative bacilli.the 2-hr quadferm+ kit (qf) (analytab products, plainview, ny) was compared with conventional tube tests for the identification of the hacek bacteria (haemophilus aphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, kingella kingae), other haemophilus and kingella spp., capnocytophaga, corynbacterium, and moraxella spp. test results were identical for 296 comparisons with 74 isolates (74%). in the remaining 104 comparisons for 26 isolates, 50 reactions ...19911873978
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...19902087341
incidence of periodontitis recurrence in treated patients with and without cultivable actinobacillus actinomycetemcomitans, prevotella intermedia, and porphyromonas gingivalis: a prospective study.a total of 98 adults previously treated for moderate to advanced periodontitis and on a trimonthly recall schedule were screened for the presence of critical levels of actinobacillus actinomycetemcomitans, prevotella (bacteroides) intermedia, and porphyromonas (bacteroides) gingivalis. patients with at least 2 positive sites were placed in a positive group and patients without or with low levels of these bacteria in a negative group. during the 30-month study the incidence of disease recurrence ...19911870068
in vitro killing of actinobacillus actinomycetemcomitans and capnocytophaga spp. by human neutrophil cathepsin g and elastase.the purpose of this study was to compare the killing of actinobacillus actinomycetemcomitans with that of capnocytophaga spp. by purified cathepsin g and elastase in vitro. both were sensitive to killing by purified cathepsin g, but only the capnocytophaga spp. were killed by elastase. killing by cathepsin g exhibited logarithmic kinetics, was enhanced slightly by alkaline ph, and was enhanced greatly under hypotonic conditions. treatment of cathepsin g with diisopropyl fluorophosphate significa ...19911879926
protective effect of porphyromonas gingivalis outer membrane vesicles against bactericidal activity of human serum.the present study was undertaken to evaluate the effect of porphyromonas gingivalis outer membrane vesicles on the bactericidal activity of human serum. human serum was pretreated with extracellular vesicles and then incubated with a cell suspension of capnocytophaga ochracea. after 2 h at 37 degrees c, the percent viability of c. ochracea was determined by cultivation on blood agar plates. at a final concentration of 0.3 mg/ml, outer membrane vesicles completely inhibited the serum bactericidal ...19911879924
rheumatoid factor (rf) distribution in periodontal disease.this study investigated the occurrence of an autoantibody, igm rheumatoid factor, that may result from the chronic inflammation noted in periodontal disease and rheumatoid arthritis. in order to detect igm-rf, a biotin-avidin elisa was developed. this assay was found to be sensitive and accurate by testing a rheumatoid arthritis population. the characteristics of this rheumatoid arthritis group were further determined, such that the total serum immunoglobulin concentrations were slightly elevate ...19911890163
dental bacterial plaques. nature and role in periodontal disease.antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ...19911890226
differential killing of actinobacillus actinomycetemcomitans and capnocytophaga spp. by human neutrophil granule components.the purpose of this study was to determine whether granule fractions of human neutrophils differentially kill actinobacillus actinomycetemcomitans and capnocytophaga spp. granule extracts were subjected to gel filtration, and seven fractions (designated a through g) were obtained. under aerobic conditions at ph 7.0, representative strains of a. actinomycetemcomitans were killed by fraction d and variably by fraction b. in contrast, the capnocytophaga spp. were killed by fractions c, d, f, and g. ...19911894375
inguinal lymphadenitis associated with capnocytophaga bacilli.capnocytophaga organisms are capnophilic, gram-negative bacilli that have been associated with infections deriving from the flora of the oropharynx. we report a case of inguinal adenitis caused by capnocytophaga species that probably represents sexual transmission of the pathogen.19911890187
[comparison of some subgingival microflora from juvenile periodontitis before and after scaling and root planing].actinobacillus actinomycetemcomitans and capnocytophaga are subgingival bacteria that have correlation with juvenile periodontitis. studies were carried out in a group of 20 patients with juvenile periodontitis before, after treatment by scaling, root planing, oral hygiene instruction and 20 patients with healthy gingiva as a control group. gingival index, plaque index, pocket depth were measured. subgingival bacteria were counted on selective media and compared by phase contrast microscopy. bef ...19911918569
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