Publications

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[therapeutic problems in acromegaly]. 20144029005
tumour antigens. 20144159818
some implications of declining birth rates for pediatrics. 2002126410
the role of health belief attitudes, sex education, and demographics in predicting adolescents' sexuality knowledge.as part of a pretest-posttest design to evaluate a 15-hour health belief model (hbm)-based sex education program, we interviewed 203 teenagers (aged 13-17) of both genders regarding their preintervention sexual and contraceptive knowledge, attitudes toward pregnancy and contraception, and prior sex education and sexual activity experiences. a multiitem sexual and contraceptive knowledge measure yielded several specific topic area scales and a total knowledge score, and a multiitem attitudes meas ...20143957688
the impact of routine admission chest x-ray films on patient care. 20144010731
[senile plaque]. 20154603819
giant rheumatoid cyst of the calf simulating a soft tissue tumour. 20113187460
[value of increased urinary excretion of modified nucleosides as tumor markers in colon cancer]. 20133811500
lipid composition of xanthomas of different types. 20154576167
[biochemical research trends and their positive value in the clinical diagnosis of schizophrenia]. 20144223861
dissection of a continuous distribution: red cell galactokinase activity in blacks.a significant difference between blacks and whites in the distribution of red cell galactokinase (galk) has been found by tedesco et al. [2]. from the shapes of the distributions, it was inferred that whites are essentially all homozygous for one allele (galka), but blacks are polymorphic. a second allele (galkp), for lower galk activity, is presented at high frequency in blacks but rare or absent in whites. this paper presents a method which, assuming the genetic model presented, estimates the ...1989209687
contact-inhibited revertant cell lines isolated from sv40-transformed cells. viii. membrane protein and glycoprotein composition. 1991208259
community assessment: a process of learning. 20051317414
anatomic basis of vascular exclusion of the liver.segmental occlusive phlebography of the ivc, coupled with a slit in its posterior wall, injection of corrosive substances into the portal and hepatocaval network, biometry of the retrohepatic ivc and serial sections of injected livers from 64 subjects allowed a study of the anatomica aspects of vel: the pringle maneuver and clamping of the ivc above and below the hepatocaval connexion. surgery for hepatic tumors close to the connexion can benefit from vel but the right suprarenal and inferior ph ...20113147531
the pathogenesis and significance of bronchial hyper-responsiveness in airways disease. 20113319356
[aids, a new disease. a nurse speaks]. 20123635242
[aids, a new disease. a nurse speaks]. 20123635242
[repair using a deltoid flap of an extensive loss of substance of the rotary cuff of the shoulder]. 20113187107
[study tour to england]. 20104106360
radioimmunoassay (ria) and other saturation assay techniques in clinical medicine. 1984215587
[ercp and endoscopic sphincterotomy in acute pancreatitis]. 20133811537
experiences with antibiotic trials in chronic bronchitis. 20164910214
teenagers' perceived and actual probabilities of pregnancy.this study sought to determine how teenagers' actual and perceived probabilities of pregnancy are related. data were collected from interviews with 425 women aged nineteen or younger who attended the door, a multiservice center for youth in new york city. subjects were asked to estimate their likelihood of becoming pregnant the last time they had intercourse, and to indicate the dates of both last intercourse and last menstrual period in order to determine their objective and subjective assessme ...20133618348
brains--a computer advisor system to aid in ct scan interpretation and cerebral disease diagnosis.this paper describes a prototype system to aid in the radiological interpretation of ct scan images of patients with cerebral disease. the system is able to provide guidance, both on diagnosis and the need for enhancement.20133587595
[studies on tuberculosis patients staying in hospitals or sanatoriums for long periods (part 1)]. 20144032944
gliding motility and por secretion system genes are widespread among members of the phylum bacteroidetes.the phylum bacteroidetes is large and diverse, with rapid gliding motility and the ability to digest macromolecules associated with many genera and species. recently, a novel protein secretion system, the por secretion system (porss), was identified in two members of the phylum, the gliding bacterium flavobacterium johnsoniae and the nonmotile oral pathogen porphyromonas gingivalis. the components of the porss are not similar in sequence to those of other well-studied bacterial secretion systems ...201323123910
helical flow of surface protein required for bacterial gliding motility.cells of flavobacterium johnsoniae and of many other members of the phylum bacteroidetes exhibit rapid gliding motility over surfaces by a unique mechanism. these cells do not have flagella or pili; instead, they rely on a novel motility apparatus composed of gld and spr proteins. sprb, a 669-kda cell-surface adhesin, is required for efficient gliding. sprb was visualized by electron microscopy as thin 150-nm-long filaments extending from the cell surface. fluorescence microscopy revealed moveme ...201323781102
identification of a new lipoprotein export signal in gram-negative bacteria.bacteria of the phylum bacteroidetes, including commensal organisms and opportunistic pathogens, harbor abundant surface-exposed multiprotein membrane complexes (sus-like systems) involved in carbohydrate acquisition. these complexes have been mostly linked to commensalism, and in some instances, they have also been shown to play a role in pathogenesis. sus-like systems are mainly composed of lipoproteins anchored to the outer membrane and facing the external milieu. this lipoprotein localizatio ...201627795390
towards a model for flavobacterium gliding.cells of flavobacterium johnsoniae, a rod-shaped bacterium about 6 μm long, do not have flagella or pili, yet they move over surfaces at speeds of about 2 μm/s. this motion is called gliding. recent advances in f. johnsoniae research include the discovery of mobile cell-surface adhesins and rotary motors. the puzzle is how rotary motion leads to linear motion. we suggest a possible mechanism, inspired by the snowmobile.201526476806
highly active and specific tyrosine ammonia-lyases from diverse origins enable enhanced production of aromatic compounds in bacteria and saccharomyces cerevisiae.phenylalanine and tyrosine ammonia-lyases form cinnamic acid and p-coumaric acid, which are precursors of a wide range of aromatic compounds of biotechnological interest. lack of highly active and specific tyrosine ammonia-lyases has previously been a limitation in metabolic engineering approaches. we therefore identified 22 sequences in silico using synteny information and aiming for sequence divergence. we performed a comparative in vivo study, expressing the genes intracellularly in bacteria ...201525911487
flavobacterium johnsoniae rema is a mobile cell surface lectin involved in gliding.cells of flavobacterium johnsoniae move rapidly over surfaces by a process known as gliding motility. gld proteins are thought to comprise the motor that propels the cell surface adhesin sprb. cells with mutations in sprb are partially defective in motility and are also resistant to some bacteriophages. transposon mutagenesis of a strain carrying a deletion spanning sprb identified eight mutants that were resistant to additional phages and exhibited reduced motility. four of the mutants had tran ...201222582276
changes of bacterioplankton apparent species richness in two ornamental fish aquaria.we analysed the 16s rrna gene diversity within the bacterioplankton community in the water column of the ornamental fish pterophyllum scalare and archocentrus nigrofasciatus aquaria during a 60-day growth experiment in order to detect any dominant bacterial species and their possible association with the rearing organisms. the basic physical and chemical parameters remained stable but the bacterial community at 0, 30 and 60 days showed marked differences in bacterial cell abundance and diversity ...201323519098
lysine and novel hydroxylysine lipids in soil bacteria: amino acid membrane lipid response to temperature and ph in pseudopedobacter saltans.microbial decomposition of organic matter is an essential process in the global carbon cycle. the soil bacteria pseudopedobacter saltans and flavobacterium johnsoniae are both able to degrade complex organic molecules, but it is not fully known how their membrane structures are adapted to their environmental niche. the membrane lipids of these species were extracted and analyzed using high performance liquid chromatography-electrospray ionization/ion trap/mass spectrometry (hplc-esi/it/ms) and h ...201526175720
[construction and application of bioinformatic analysis platform for aquatic pathogen based on the milkyway-2 supercomputer].as a key component of life science, bioinformatics has been widely applied in genomics, transcriptomics, and proteomics. however, the requirement of high-performance computers rather than common personal computers for constructing a bioinformatics platform significantly limited the application of bioinformatics in aquatic science. in this study, we constructed a bioinformatic analysis platform for aquatic pathogen based on the milkyway-2 supercomputer. the platform consisted of three functional ...201526351170
bacteria that glide with helical tracks.many bacteria glide smoothly on surfaces, despite having no discernable propulsive organelles on their surface. recent experiments with myxococcus xanthus and flavobacterium johnsoniae show that both of these distantly related bacterial species glide using proteins that move in helical tracks, albeit with significantly different motility mechanisms. both species utilize proton-motive force for movement. although the motors that power gliding in m. xanthus have been identified, the f. johnsoniae ...201424556443
comparative analysis of cellulophaga algicola and flavobacterium johnsoniae gliding motility.gliding motility is common in members of the phylum bacteroidetes, including flavobacterium johnsoniae and cellulophaga algicola. f. johnsoniae gliding has been extensively studied and involves rapid movement of the cell surface adhesin sprb. genetic analysis of c. algicola allowed a comparative analysis of gliding. sixty-three himarem1-induced mutants that formed nonspreading colonies were characterized. each had an insertion in an ortholog of an f. johnsoniae motility gene, highlighting simila ...201627044627
molecular characterization of a cold-active recombinant xylanase from flavobacterium johnsoniae and its applicability in xylan hydrolysis.a novel xylanase gene, xyn10a, was cloned from flavobacterium johsoniae, overexpressed in a flavobacterial expression system, the recombinant enzyme purified by ni-affinity chromatography, and enzyme structure and activity analyzed. xyn10a was found to be a modular xylanase with an fn3 accessory domain on its n-terminal and a catalytic region on the c-terminal. the optimum ph and temperature for xyn10a was 8.0 and 30 °c, but xyn10a retained 50% activity at 4 °c, indicating that xyn10a is a cold- ...201223196234
involvement of the type ix secretion system in capnocytophaga ochracea gliding motility and biofilm formation.capnocytophaga ochracea is a gram-negative, rod-shaped bacterium that demonstrates gliding motility when cultured on solid agar surfaces. c. ochracea possesses the ability to form biofilms; however, factors involved in biofilm formation by this bacterium are unclear. a type ix secretion system (t9ss) in flavobacterium johnsoniae was shown to be involved in the transport of proteins (e.g., several adhesins) to the cell surface. genes orthologous to those encoding t9ss proteins in f. johnsoniae ha ...201626729712
identification and characterization of cellobiose 2-epimerases from various aerobes.cellobiose 2-epimerase (ce), found mainly in anaerobes, reversibly converts d-glucose residues at the reducing end of β-1,4-linked oligosaccharides to d-mannose residues. in this study, we characterized ce-like proteins from various aerobes (flavobacterium johnsoniae nbrc 14942, pedobacter heparinus nbrc 12017, dyadobacter fermentans atcc 700827, herpetosiphon aurantiacus atcc 23779, saccharophagus degradans atcc 43961, spirosoma linguale atcc 33905, and teredinibacter turnerae atcc 39867), beca ...201323291764
draft genome sequence of flavobacterium johnsoniae ci04, an isolate from the soybean rhizosphere.flavobacterium johnsoniae ci04 was coisolated with bacillus cereus from a root of a field-grown soybean plant in arlington, wi, and selected as a model for studying commensalism between members of the cytophaga-flavobacterium-bacteroides group and b. cereus here we report the draft genome sequence of f. johnsoniae ci04 obtained by illumina sequencing.201728126949
discovery of a bifunctional acyltransferase responsible for ornithine lipid synthesis in serratia proteamaculans.ornithine lipids (ols) are phosphorus-free membrane lipids that can be formed by many bacteria but that are absent from archaea and eukaryotes. a function for ols in stress conditions and in host-bacteria interactions has been shown in some bacteria. some bacterial species have been described that can form ols, but lack the known genes (olsba) involved in its biosynthesis, which implied the existence of a second pathway. here we describe the bifunctional protein olsf from serratia proteamaculans ...201525040623
a glycoside hydrolase family 31 dextranase with high transglucosylation activity from flavobacterium johnsoniae.glycoside hydrolase family (gh) 31 enzymes exhibit various substrate specificities, although the majority of members are α-glucosidases. here, we constructed a heterologous expression system of a gh31 enzyme, fjoh_4430, from flavobacterium johnsoniae nbrc 14942, using escherichia coli, and characterized its enzymatic properties. the enzyme hydrolyzed dextran and pullulan to produce isomaltooligosaccharides and isopanose, respectively. when isomaltose was used as a substrate, the enzyme catalyzed ...201627170214
a polysaccharide utilization locus from flavobacterium johnsoniae enables conversion of recalcitrant chitin.chitin is the second most abundant polysaccharide on earth and as such a great target for bioconversion applications. the phylum bacteroidetes is one of nature's most ubiquitous bacterial lineages and is essential in the global carbon cycle with many members being highly efficient degraders of complex carbohydrates. however, despite their specialist reputation in carbohydrate conversion, mechanisms for degrading recalcitrant crystalline polysaccharides such as chitin and cellulose are hitherto u ...201627933102
the screw-like movement of a gliding bacterium is powered by spiral motion of cell-surface adhesins.flavobacterium johnsoniae, a rod-shaped bacterium, glides over surfaces at speeds of ∼2 μm/s. the propulsion of a cell-surface adhesin, sprb, is known to enable gliding. we used cephalexin to generate elongated cells with irregular shapes and followed their displacement in three dimensions. these cells rolled about their long axes as they moved forward, following a right-handed trajectory. we coated gold nanoparticles with an sprb antibody and tracked them in three dimensions in an evanescent fi ...201627602728
flavobacterium gliding motility and the type ix secretion system.cells of flavobacterium johnsoniae crawl rapidly over surfaces in a process called gliding motility. these cells do not have flagella or pili but instead rely on a novel motility machine composed of proteins that are unique to the phylum bacteroidetes. the motility adhesins sprb and rema are propelled along the cell surface by the still poorly-defined gliding motor. interaction of these adhesins with a surface results in translocation of the cell. sprb and rema are delivered to the cell surface ...201526461123
improved biostability assessment of drinking water with a suite of test methods at a water supply treating eutrophic lake water.assessment of drinking-water biostability is generally based on measuring bacterial growth in short-term batch tests. however, microbial growth in the distribution system is affected by multiple interactions between water, biofilms and sediments. therefore a diversity of test methods was applied to characterize the biostability of drinking water distributed without disinfectant residual at a surface-water supply. this drinking water complied with the standards for the heterotrophic plate count a ...201526451977
a rotary motor drives flavobacterium gliding.cells of flavobacterium johnsoniae, a rod-shaped bacterium devoid of pili or flagella, glide over glass at speeds of 2-4 μm/s [1]. gliding is powered by a protonmotive force [2], but the machinery required for this motion is not known. usually, cells move along straight paths, but sometimes they exhibit a reciprocal motion, attach near one pole and flip end over end, or rotate. this behavior is similar to that of a cytophaga species described earlier [3]. development of genetic tools for f. john ...201525619763
flavobacterium johnsoniae porv is required for secretion of a subset of proteins targeted to the type ix secretion system.flavobacterium johnsoniae exhibits gliding motility and digests many polysaccharides, including chitin. a novel protein secretion system, the type ix secretion system (t9ss), is required for gliding and chitin utilization. the t9ss secretes the cell surface motility adhesins sprb and rema and the chitinase chia. proteins involved in secretion by the t9ss include gldk, gldl, gldm, gldn, spra, spre, and sprt. porphyromonas gingivalis has orthologs for each of these that are required for secretion ...201525331433
bidirectional bacterial gliding motility powered by the collective transport of cell surface proteins.the gliding motility of flavobacterium johnsoniae is driven by moving surface adhesive proteins. recently, these motility components were observed to travel along a closed loop on the cell surface. the mechanism by which such moving surface adhesins give rise to cell motion remains unknown. on the basis of the unique motility properties of f. johnsoniae, we present a generic model for bidirectional motion of rigidly coupled adhesins, which are propelled in opposite directions. using analytical a ...201324483703
flavobacterium johnsoniae chitinase chia is required for chitin utilization and is secreted by the type ix secretion system.flavobacterium johnsoniae, a member of phylum bacteriodetes, is a gliding bacterium that digests insoluble chitin and many other polysaccharides. a novel protein secretion system, the type ix secretion system (t9ss), is required for gliding motility and for chitin utilization. five potential chitinases were identified by genome analysis. fjoh_4555 (chia), a 168.9-kda protein with two glycoside hydrolase family 18 (gh18) domains, was targeted for analysis. disruption of chia by insertional mutage ...201424363341
a retrospective analysis of patient falls. 20144005770
epidural fentanyl at 4 degrees c for analgesia after epidural caesarean section. 20092757170
[suppliers are obliged to give instruction in swedish]. 1991258066
deletion of the cytophaga hutchinsonii type ix secretion system gene sprp results in defects in gliding motility and cellulose utilization.cytophaga hutchinsonii glides rapidly over surfaces and employs a novel collection of cell-associated proteins to digest crystalline cellulose. himarem1 transposon mutagenesis was used to isolate a mutant with an insertion in chu_0170 (sprp) that was partially deficient in gliding motility and was unable to digest filter paper cellulose. sprp is similar in sequence to the porphyromonas gingivalis type ix secretion system (t9ss) protein porp that is involved in the secretion of gingipain protease ...201424257839
isolation and identification of phytate-degrading bacteria and their contribution to phytate mineralization in soil.to better understand the phosphorus (p) cycling in an agricultural soil environment, amounts of total, organic and inorganic p in 10 agricultural soil samples were analyzed. since a large proportion (57.8%) of the total p in the soils was in organic form, a method was developed to evaluate the mineralization rate of organic p in the soil by adding phytate to the soil and analyzing the change in water-soluble p (wsp) content after incubating it for 3 days. moreover, the relationship between the p ...201324201147
raman spectrometric discrimination of flexirubin pigments from two genera of bacteroidetes.flexirubins are specific polyene pigments produced by several genera of bacteroidetes. colonies and cell extracts of flavobacterium johnsoniae and flexibacter elegans have been investigated by raman spectroscopy to show that this fast and non-destructive technique can be used to differentiate these pigments from carotenoids and to compare the flexirubin content of the two microorganisms. the presence or absence of certain distinguishing features in the ch combination band region at 2500-2750 cm( ...201324033756
flavobacterium johnsoniae gldk, gldl, gldm, and spra are required for secretion of the cell surface gliding motility adhesins sprb and rema.flavobacterium johnsoniae cells move rapidly over surfaces by gliding motility. gliding results from the movement of adhesins such as sprb and rema along the cell surface. these adhesins are delivered to the cell surface by a bacteroidetes-specific secretion system referred to as the type ix secretion system (t9ss). gldn, spre, sprf, and sprt are involved in secretion by this system. here we demonstrate that gldk, gldl, gldm, and spra are each also involved in secretion. nonpolar deletions of gl ...201323667240
the mercury resistance (mer) operon in a marine gliding flavobacterium, tenacibaculum discolor 9a5.genes conferring mercury resistance have been investigated in a variety of bacteria and archaea but not in bacteria of the phylum bacteroidetes, despite their importance in many environments. we found, however, that a marine gliding bacteroidetes species, tenacibaculum discolor, was the predominant mercury-resistant bacterial taxon cultured from a salt marsh fertilized with mercury-contaminated sewage sludge. here we report characterization of the mercuric reductase and the narrow-spectrum mercu ...201322816663
flavobacterium tilapiae sp. nov., isolated from a freshwater pond, and emended descriptions of flavobacterium defluvii and flavobacterium johnsoniae.a bacterial strain, designated ruye-71(t), was isolated from a freshwater tilapiine cichlid fish culture pond in taiwan and characterized in a taxonomic study using a polyphasic approach. cells of strain ruye-71(t) were gram-stain-negative, strictly aerobic, yellow-pigmented rods that were motile by gliding. growth occurred at 10-30 °c (optimum, 15-30 °c), at ph 7.0-8.0 (optimum, ph 8.0) and with 0-2 % nacl (optimum, 0-1 %). phylogenetic analyses based on 16s rrna gene sequences showed that stra ...201322611198
the volatile-producing flavobacterium johnsoniae strain gse09 shows biocontrol activity against phytophthora capsici in pepper.previously, we selected a bacterial strain (gse09) antagonistic to phytophthora capsici on pepper, which produced a volatile compound (2,4-di-tert-butylphenol), inhibiting the pathogen. in this study, we identified strain gse09 and characterized some of the biological traits of this strain in relation to its antagonistic properties against p. capsici. in addition, we examined bacterial colonization on the root surface or in rhizosphere soil and the effect of various concentrations of the volatil ...201222563881
epilactose production by 2 cellobiose 2-epimerases in natural milk.it was reported recently that cellobiose 2-epimerases (ce) from various aerobic microorganisms convert lactose to epilactose in defined buffer systems. in this study, we showed that ce from 2 mesophilic microorganisms, flavobacterium johnsoniae and pedobacter heparinus, were capable of converting lactose to prebiotic epilactose not only in buffer but also in a complex milk system. first, the 2 enzymes were separately cloned, recombinantly expressed in escherichia coli, and purified by column chr ...201424210487
exploiting members of the bahd acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast.bahd acyltransferases, named after the first four biochemically characterized enzymes of the group, are plant-specific enzymes that catalyze the transfer of coenzyme a-activated donors onto various acceptor molecules. they are responsible for the synthesis in plants of a myriad of secondary metabolites, some of which are beneficial for humans either as therapeutics or as specialty chemicals such as flavors and fragrances. the production of pharmaceutical, nutraceutical and commodity chemicals us ...201627871334
developmental trajectories of amphibian microbiota: response to bacterial therapy depends on initial community structure.improving host health through microbial manipulation requires untangling factors that shape the microbiome. there is currently little understanding of how initial community structure may drive the microbiota trajectory across host development or influence bacterial therapy outcomes. probiotic baths of surface symbionts, pseudomonas fluorescens and flavobacterium johnsoniae were administered to 240 tadpoles of the midwife toad, alytes obstetricans in semi-natural outdoor mesocosms originating fro ...201728229543
the type ix secretion system (t9ss): highlights and recent insights into its structure and function.protein secretion systems are vital for prokaryotic life, as they enable bacteria to acquire nutrients, communicate with other species, defend against biological and chemical agents, and facilitate disease through the delivery of virulence factors. in this review, we will focus on the recently discovered type ix secretion system (t9ss), a complex translocon found only in some species of the bacteroidetes phylum. t9ss plays two roles, depending on the lifestyle of the bacteria. it provides either ...201728603700
untangling flavobacterium johnsoniae gliding motility and protein secretion.flavobacterium johnsoniae exhibits rapid gliding motility over surfaces. at least 20 genes are involved in this process. seven of these, gldk, gldl, gldm, gldn, spra, spre, and sprt, encode proteins of the type ix protein secretion system (t9ss). the t9ss is required for surface localization of the motility adhesins sprb and rema, and for secretion of the soluble chitinase chia. here, we demonstrate that the gliding motility proteins glda, gldb, gldd, gldf, gldh, gldi, and gldj are also essentia ...201829109184
identification of enzymes involved in the mevalonate pathway of flavobacterium johnsoniae.the mevalonate pathway is prevalent in eukaryotes, archaea, and a limited number of bacteria. this pathway yields the fundamental precursors for isoprenoid biosynthesis, i.e., isopentenyl diphosphate and dimethylally diphosphate. in the downstream part of the general eukaryote-type mevalonate pathway, mevalonate is converted into isopentenyl diphosphate by the sequential actions of mevalonate kinase, phosphomevalonate kinase, and diphosphomevalonte decarboxylase, while a partial lack of the puta ...201728442346
diverse c-terminal sequences involved in flavobacterium johnsoniae protein secretion.flavobacteriumjohnsoniae and many related bacteria secrete proteins across the outer membrane using the type ix secretion system (t9ss). proteins secreted by t9sss have amino-terminal signal peptides for export across the cytoplasmic membrane by the sec system and carboxy-terminal domains (ctds) targeting them for secretion across the outer membrane by the t9ss. most but not all t9ss ctds belong to the family tigr04183 (type a ctds). we functionally characterized diverse ctds for secretion by th ...201728396348
more than gliding: involvement of gldd and gldg in the virulence of flavobacterium psychrophilum.a fascinating characteristic of most members of the genus flavobacterium is their ability to move over surfaces by gliding motility. flavobacterium psychrophilum, an important pathogen of farmed salmonids worldwide, contains in its genome the 19 gld and spr genes shown to be required for gliding or spreading in flavobacterium johnsoniae; however, their relative role in its lifestyle remains unknown. in order to address this issue, two spreading deficient mutants were produced as part of a tn4351 ...201729163446
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