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the use of trypan blue for counterstaining in the sepharose bead immunofluorescence test. the application of the test for the demonstration of primate retrovirus-specific antibodies and antigens.the sepharose bead immunoflurorescence test was performed by counterstaining the beads with trypan blue. this results in a red staining of negative beads which allows an easy distinction from positive green-fluorescent beads. sepharose beads conjugated with viral proteins or antiviral antibodies were used to demonstrate mason-pfizer monkey virus (mpmw)- and simian sarcoma virus (ssv) - specific antigens or antibodies. the test shows a high sensitivity and specificity and needs a small amount of ...197886253
the zinc-iodide-osmium technique in studies of the neurosecretory system in rats. 197991310
a new fluorescence technique for staining of mononuclear phagocytes.a recently developed fluorochrome, 4',6-diamidino-2-phenylindole (dapi), is used to stain mononuclear phagocytes of the mouse. after addition of heparin, these cells showed a bright yellow outer ring.197991397
condensation--inhibition by 33258-hoechst of centromeric heterochromatin in prematurely condensed mouse chromosomes. 197991529
immunological specificity of natural opsonins and their role in the cross-reactivity between staphylococcus aureus mardi and escherichia coli 101.the immunochemical specificity of the observed cross-reactivity between escherichia coli strain 101 and staphylococcus aureus strain mardi was examined. the cross-reactivity was shown to be dependent upon mucopeptide antibodies which are present in normal and immune sera. although both organisms contained surface antigens with immunodominant glucuronic acid residues, in vitro phagocytosis studies indicated that antibodies directed against these antigens were not significantly involved in the ops ...197991583
site-specific anti-cytochrome c antibodies. inhibition of the reactions between cytochrome c and its respiratory chain electron exchange partners. 197991615
effect of various histological fixatives on fluorescent antibody detection of legionnaires disease bacteria.human lung tissue containing the legionnaires disease bacterium was fixed in seven different histological fixatives, processed, and embedded in paraffin. deparaffined sections from each were stained by fluorescent antibody and by dieterle silver impregnation. with the fluorescent antibody stain, the legionnaires disease bacterium could be detected in tissues prepared with any of the fixatures, but the dieterle silver impregnation was not satisfactory on zenker-fixed tissues.197991621
immunochemical evidence for multiple serotypes of bacteroides fragilis.an immunochemical comparison of outer membrane antigens obtained from five select and biochemically defined strains indicated that there are several serotypes of bacteroides fragilis. each strain was serologically defined by individual or by combinations of determinant groups composed of carbohydrates in the form of polysaccharides or glycoproteins. the carbohydrate constituents were tentatively identified as glucose, galactose, fucose, rhamnose, glucosamine, galactosamine, and traces of mannose ...197991622
the murine kupffer cell. i. characterization of the cell serving accessory function in antigen-specific t cell proliferation.murine kupffer cells, the tissue macrophages of the liver, were isolated by collagenase digestion, differential sedimentation over metrizamide, and glass adherence. the resultant cell population was more than 86% phagocytic, and 95% of cells stained positively for alpha-naphthyl butyrate esterase activity. the cells also had cell surface receptors for complement (c) and the fc portion of igg. in addition, a large proportion of kupffer cells was shown to bear ia antigens: about half of the cells ...197991637
sensitivity of the short-range spinal interneurons of the cat to experimental spinal cord trauma.the technique of retrograde axoplasmic transport was used to demonstrate the effect of experimental spinal cord injury on the spinal interneurons in the upper lumbar and lower thoracic segments of cats. force of varied intensity was applied to the dorsal surface of the spinal cord and horseradish peroxidase (hrp) was injected into the next caudal segment. a large impact (250 to 350 gm-cm) inducing permanent paraplegia of the hind legs blocked the axoplasmic transport instantaneously in both cran ...197991677
production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2.we prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. cell lines 1d4 and 3e1, respectively, secreted immunoglobulin g1 herpes simplex virus type 1 and immunoglobulin g1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. in contrast, guinea pig antisera prepared against either herpes sim ...197991687
b-cell activation in angioimmunoblastic lymphadenopathy after immunisation with multivalent pneumococcal polysaccharide vaccine. 197991879
[chemical composition of the cell wall of streptomyces chrysomallus which produces the antibiotic aurantin].the cell wall chemical composition of streptomyces chrysomallus producing the antibiotic aurantin was studied. the cell wall represents 11--13% of the dry cell weight and contains three major polymers, namely, peptidoglycan, ribitol teichoic acid and a polysaccharide. each muramic acid of peptidoglycan has a peptide subunit which consists of two alanine residues, glutamic acid and diaminopimelic acid residues. peptide chains are connected with bridges consisting of one glycine residue. the poly( ...197991941
extensive sequence homology at the 3'-termini of the four rnas of cucumber mosaic virus.the sequences of 270 residues from the 3'-termini of the four rnas of cucumber mosaic virus have been determined by copying the in vitro polyadenylated rnas with reverse transcriptase using d(pt8g) as primer and the 2',3'-dideoxynucleoside 5'-triphosphates as specific chain terminators. the terminal sequences of rnas 3 and 4 were identical; this was expected since hybridization data has shown that the sequence of rna 4 was present at the 3'-end of rna 3 (gould and symons (1978) eur. j. biochem. ...197992011
vitamin k deficiency in male rats fed diets containing butylated hydroxytoluene (bht). 197992074
serological cross-reactions of leptospiral lipopolysaccharide (f4) antigen.the serological specificity of leptospiral lipopolysaccharide (f4) antigen was examined by the technique of passive haemagglutination. f4 extracted from leptospiral serovars representative of several different serogroups showed wide cross reaction between serovars, including numerous one-way (non-reciprocal) reactions. the pattern of cross reaction was different to that of the standard leptospiral classification scheme.197992115
the o antigenic relationships of kloeckera africana, salmonella aberdeen and escherichia coli o75.the immune sera for salmonella aberdeen (o11) and escherichia coli (o75) agglutinated kloeckera africana strain. the immune serum for k. africana agglutinated s. aberdeen (o11) and e. coli (o75) strains. absorption and agglutination cross-tests demonstrated common antigenic factor(s) in the tested yeast and salmonella o11 and e. coli o75 antigens.197992211
purification of rna polymerase from actinomycin producing and nonproducing cells of streptomyces antibioticus. 197992215
amino-acid sequence analysis of the amino-terminal tryptic peptides of different actins from the same mammal [proceedings]. 197992280
amino acid sequence analysis of group e allotype-related peptides derived from lagomorph igg.a tryptic peptide (t 19) was isolated from a cnbr fragment of igg from several representatives of present-day lagomorphs including domestic rabbit, hare, cottontail rabbit and pika. the amino acid sequence of this peptide revealed differences which can be correlated with serological polymorphism associated with group e igg allotypes. the results confirm previous studies establishing the importance of ala309 for the expression of e 15, particularly the i subspecificity. arg315 correlates with the ...197992412
generation of virus-specific cytotoxic t cells in vitro. i. induction conditions of primary and secondary sendai virus-specific cytotoxic t cells.h-2-restricted cytotoxic t cells specific for sendai virus were generated in vitro in a primary response from normal mouse lymphocytes cultured in the presence of infective as well as inactivated sendai virus. antigen-presenting cells of different origin, including t cells, were found to be effective stimulators. antibodies to sendai virus were shown to inhibit the activation of specific precursor killer cells when added to cultures before, but not after, the addition of viral antigen. data obta ...197992413
non-mutagenicity for salmonella of the chlorinated hydrocarbons aroclor 1254, 1,2,4-trichlorobenzene, mirex and kepone.a polychlorinated biphenyl mixture, aroclor 1254, two commercial grade insecticides, mirex and kepone, and a pesticide breakdown product, 1,2,4-trichlorobenzene were evaluated for mutagenicity and hepatic enzyme induction potential in the salmonella/microsomal assay. none was found to revert strains ta1535, ta1537, ta98 or ta100 when tested with or without metabolic activation. liver microsomal extracts (s9) from rats induced with 1,2,4-trichlorobenzene were shown to differ from s9 of either con ...197992763
effect of escherichia coli endotoxin on the developing rat liver. i. giant cell induction and disruption in protein metabolism. 197992781
the role of mite allergen in chronic urticaria.fifty-two patients with chronic urticaria were tested intradermally with mite allergen (dermatophagoides farinae). none had any past history of any other atopic diseases. thirty of the 52 patients gave positive skin tests to mite allergen. a good correlation between skin sensitivity to mite allergen and in vitro mite antigen-induced histamine release from leucocytes was observed. these facts suggest that a mite may be an important role in chronic urticaria.197992903
[counterimmunoelectrophoresis on cellulose acetate membrane with a commercial lyophilized antigen in diagnosis of human hydatidosis].a very simplified method of crossed over electrophoresis (ciep) was employed with a lyophilized commercially produced antigen (previously submitted to several freezing and thawing) and cellulose acetate membrane, for the diagnosis of human hydatidosis. results are as follows: active hydatidosis (surgically confirmed): number of sera tested 35; positive 32 (91%). sera of patients with parasitic and non parasitic illnesses (especially malignancies of liver and lung) did not show any precipitin lin ...197992994
preferential induction of memory t cells for delayed-type hypersensitivity with reduced and alkylated human serum albumin in mice.reduction and alkylation of human serum albumin (hsa) resulted in molecular aggregation of the protein. the reduced and alkylated antigen (ra-hsa) was lacking in the ability to induce delayed-type hypersensitivity (dth) response as well as antibody response to native hsa in mice, although native hsa induced both responses. on the other hand, ra-hsa could stimulate a priming function that accelerated and enhanced the dth response to native hsa, which, however, failed to stimulate the function. th ...197993084
characterization of two pregnancy associated serum proteins with pregnancy specific beta 1-glycoprotein (ps-beta-g)-determinants.the existence of two pregnancy associated, partially identical glycoproteins with ps-beta-g determinants were demonstrated in serum from pregnant women. crossed and crossed-line immunoelectrophoretic analysis of pregnancy sera revealed two precipitates with alpha- and beta-mobility, respectively. the ratio between the precipitation areas in various samples could be related to three different types of precipitation patterns observed in rocket immunoelectrophoresis. this variation in precipitation ...197993301
[effects of the phospholipid environment and its composition on n-acetyl-muramyl-l-alanine amidase activity (proceedings)]. 197993468
measurement and characterization of estrogen receptors in the human prostate. 197993752
two independent hla-b-locus specificities expressed on a single haplotype. 197993796
priming increases the amount of interferon mrna in poly(ri).poly(rc)-treated l cells.priming by mouse interferon pre-treatment resulted in an accumulation of interferon mrna in poly(ri).poly(rc)-treated l cells, starting early in the period of interferon synthesis. on electrophoresis, the priming activity of an interferon preparation co-migrated with the antiviral activity, which suggests identity of the functional principle(s) for these activities.197994345
antigenicity of erythrocytes as analyzed in terms of their cross-reactivity.the antigenicity of human erythrocytes of four different abo blood groups and sheep erythrocytes of unknown blood type from different individual sheep were analyzed in terms of their cross-reactivity with antibody-producing cells (plaque-forming cells, pfc) and serum antibody in immunized c57bl/6 and c3h/he mice. the antigenicity of human erythrocytes of different abo blood groups in the c57bl/6 mice, as determined by the number of specific pfc, was, in decreasing order, ab = a greater than b = ...197994423
the effect of glutaurine on the development of amphibian larvae with inhibited rna and protein synthesis.1. glutaurine (gamma-l-glutamyl-taurine) isolated from bovine parathyroid and later synthetized retards the metamorphosis of rana arvalis. 2. from day 13 of treatment glutaurine moderates the metamorphosis inhibiting effect of antibiotics acting at the transcription and translation level.197994855
[the effects of bleomycin on chemically induced squamous cell carcinoma in oral membrane of rats (author's transl)]. 197895058
immunofluorescence and electron microscopic studies on kidney, choroid plexus and pituitary in rhesus monkeys immunized with the anti-hcg vaccine pr-beta-hcg-tt. 197898291
improved safety of splenoportography by plugging of the needle tract.splenoportography, a useful preoperative diagnostic procedure in patients with portal hypertension, has been largely discontinued because of the risk of hemorrhage. we have used both ivalon and gelfoam in particle and plug form to plug the needle tract after splenoportography. initial studies were conducted on six dogs. plugging of the needle tract by injection of suspensions of small gelfoam or ivalon particles resulted in intravasation of these particles into the splenic vein. this was demonst ...197898987
[constant and intermittent inhalatory effects of sevin on body regulatory systems in animals]. 1978101422
effect of testosterone propionate on electrical activity of brain in intact & gonadectomized rhesus monkeys. 1978101442
comparison of antigens of pneumonia-associated mycoplasma species by gel diffusion.comparison of fluorocarbon-extracted antigens of six mycoplasma species by double immunodiffusion and counterimmunodiffusion techniques revealed a close reciprocal relationship among mycoplasma dispar, m. ovipneumoniae, and m. hyopneumoniae. a lesser degree of cross-reaction was also demonstrated between these three species and m. hyorhinis and m. bovoculi. the interrelationships were more clearly demonstrated by double immunodiffusion than by counterimmunodiffusion.1978101469
congenital defects due to hyperthermia. 1978104560
detailed monitoring of the effects of mannitol following experimental head injury.the experimental model of a cerebral missile injury developed by crockard was used in three groups of rhesus monkeys treated with mannitol. one group received mannitol 15 minutes after being injured with a bb pellet at 90 m/sec impact. another group was wounded identically, but mannitol treatment was delayed until 1 hour after injury. the last group was wounded with the missile traveling at 180 m/sec, and mannitol was started 15 minutes after trauma. the data were contrasted with the results fro ...1979106095
[bacillus cereus septicemia (author's transl)]. 1978106146
absence of mutagenicity of praziquantel, a new, effective, anti-schistosomal drug, in bacteria, yeasts, insects and mammalian cells.praziquantel (embay 8440, droncit) a new, effective anti-schistosomal drug, was tested in various short-term assays that have shown a predictive value for the detection of potential carcinogens. indicator organisms s. typhimurium strains, s. pombe, s. cerevisiae, cultured v79 chinese hamster cells or human heteroploid cells and drosophila melanogaster were treated with praziquantel. the induction of reverse and forward mutations, mitotic gene conversions, x-linked recessive lethals, sister-chrom ...1978106268
domains and the hinge region of an immunoglobulin heavy chain are encoded in separate dna segments.a 6.8-kilobase dna fragment containing the sequence coding for the constant region of the mouse immunoglobulin gamma1 heavy chain was cloned from total cellular dna. electron microscopic and nucleotide sequencing studies showed that the three protein domains and the hinge region are encoded in separate dna segments.1979106304
the pontocerebellar projection in the rhesus monkey: an experimental study with retrograde axonal transport of horseradish peroxidase. 1979106327
[differences in the ependyma of lagomorpha and primates: scanning electron microscopy observations of the inferior horn of the lateral ventricle in rabbits and monkeys]. 1978106571
thyroid hormone-aldosterone interaction on na+ transport in toad bladder.repeated administration of thyroxine (t4) in vivo (2 microgram/100 g body wt for 6 days) lowered by 2.3 times (p less than 0.025, df = 18) the basal rate of na+ transport measured by the short-circuit current (scc) in vitro in the urinary bladder of the toad (bufo marinus). this difference was not accounted for by a change in the plasma aldosterone concentration. moreover the response of the scc to aldosterone in vitro was markedly inhibited in bladders from t4-treated animals (p less than 0.001 ...1979106733
activation and inhibition of the mg-ca-atpase from e. coli by mg2+ and ca2+.mgatp of caatp is the substrate of the mg-ca-atpase. at low mg2+- or ca2+-concentrations the atpase is activated by mg2+ or ca2+, the activator being essential for activity. at higher mg2+- or ca2+-concentrations the mg-ca-atpase is inhibited competitively. thus the real km is smaller than reported in the literature. h+ competes with mg2+ or ca2+ for the metal binding sites.1975126584
purification and characterization of (na+, k+)-atpase. v. conformational changes in the enzyme transitions between the na-form and the k-form studied with tryptic digestion as a tool.1. purified (na+, k+)-atpase consisting of membrane fragments was digested with trypsin. the time course of enzyme inactivation was related to the electrophoretic pattern of native and cleaved proteins remaining in the membrane. 2. differences in both the inactivation kinetics and the cleavage of the large chain (mol. wt 98 000) allow distinction of two patterns of tryptic digestion of (na+, k+)-atpase seen with na+ or k+ in the medium. 3. with k+, the inactivation of (na+, k+)-atpase is linear ...1975126698
dysplasia of the tricuspid valve in the dog and cat.dysplasia of the tricuspid valve in 14 dogs and 13 cats was studied. the clinical, electrocardiographic, radiographic, hemodynamic, angiocardiographic, and pathologic findings were reviewed in each species. alterations of the tricuspid valve complex included long, thick septal leaflets adhered to the septum; absent or short, stout fused chordae tendineae; hypertrophic fused papillary muscles; insertion of papillary muscles directly into the lateral leaflets; incomplete development of the valvula ...1976134984
the pathology of leprosy. 1978152980
biochemical properties of brain capillaries. 1977153001
a fifth gene (unce) in the operon concerned with oxidative phosphorylation in escherichia coli.three mutant unc alleles (unc-408, unc-410, and unc-429) affecting the coupling of electron transport to oxidative phosphorylation in escherichia coli k-12 have been characterized. genetic complementation analyses using previously defined mutant unc alleles indicated that the new mutant unc alleles affect a previously undescribed gene designated unce. the phenotype of strains carrying the unce408 or unce429 allele is similar in that mg(2+)-adenosine triphosphatase activity is only found in the c ...1979154509
significance of central norepinephrine-containing neurons in sensory transmission. 1975165143
bone changes in congenital cytomegalic inclusion disease.1. while below 10 degrees c, the initial burst of pi liberation in the hydrolysis of mn(ii)-atp by heavy meromyosin or myosin subfragment 1 was inhibited by the pre-addition of adp without any change in the steady-state activity, it was not inhibited above 10 degrees c. the burst size was about one mole per two moles of myosin active sites. 2. above 10 degrees c, the ultraviolet absorption spectrum of heavy meromyosin induced by atp in mnc12 was similar to that induced in mgc12 and the spectral ...1975165786
nitric oxide complexes of cytochrome p-450. 1975166885
glycosaminoglycans and glycoproteins isolated from rabbit femur.1. complex carbohydrate fractions were extracted successively with 40% aqueous edta (ph 7.4) and 6m urea (ph 7.8) from acetone-dried bone powder of rabbit femur. 2. the carbohydrate fraction extracted with edta (e=fr) was separated into five fractions,d1approximatelyd5 by deae-dephadex a-50 column chromatography. chemical and infrared spectral analyses, and enzymatic digestion indicate that d2 contained lessacidic glycoprotein, d3 contained sialoglycoprotein, d4 contained a low sulfated proteoke ...1975168183
characterization of sv40 dna rescued from transformed mouse cells. 1975169101
preliminary crystallographic study on cytochrome c3 of desulfovibrio desulfuricans (strain norway). 1976181583
effect of radiation therapy on facial nerve cable autografts. 1976183328
purification and characterization of cytochrome c3, ferredoxin, and rubredoxin isolated from desulfovibrio desulfuricans norway.different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium desulfovibrio desulfuricans (norway strain) have been studied. two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. this ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six residues of cysteine per molecule. its amino acid composition suggests that it is homologous with the other desulfovibrio ferredoxins. the rubredoxin is also an acidic protein of 6,000 m ...1977187570
evidence for hydroxyl radical generation by human monocytes.a number of highly reactive oxygen species have been implicated in the oxygen-dependent mechanisms involved in bactericidal activity of phagocytic leukocytes. hydrogen peroxide and superoxide, two agents known to occur during phagocytosis, are thought to interact to generate hydroxyl radical, singlet oxygen, and other potentially reactive molecules. using an assay system of ethylene generation from methional, cell preparations of human monocytes were demonstrated to generate hydroxyl radical or ...1977194926
effect of erythropoietin preparations on cyclic amp and cyclic gmp levels in rat fetal liver cell cultures. 1977194988
use of immunoperoxidase for rapid diagnosis of mucocutaneous herpes simplex virus infection.the indirect immunoperoxidase method was applied to the rapid diagnosis of mucocutaneous herpes simplex virus infection. specimens were obtained directly from lesions at various stages in their evolution. the immunoperoxidase method identified herpes simplex virus-infected cells in 91% of vesicular lesions which were positive by standard virological culture. at later stages, the number of cases that could be diagnosed decreased appreciably. the immunoperoxidase method appears to be a rapid and r ...1977201662
[bone tissue collagen and vitamin d in chronic kidney insufficiency in rats].chronic kidney insufficiency, caused by subtotal nephrectomy, did not lead to distinct alterations in hydroxyproline concentration in blood and to its excretion in rats within 1-1.5 months after the operation. content of salt-soluble collagen was decreased in diaphyses of rat tubular bones in chronic kidney insufficiency; in the epiphyses (with metaphyses) content of the collagen fraction was increased, but the concentration of non-soluble collagen was unaltered. decrease in content of salt-solu ...1977202093
quantitative determination of myoinositol, inositol 1-phosphate, inositol cyclic 1 : 2-phosphate and glycerylphosphoinositol in normal and rous-sarcoma-virus-transformed quail fibroblasts under different growth conditions.myoinositol and its phosphorylated derivatives have been quantitatively determined in normal and rous-sarcoma-virus-transformed quail cells under various growth conditions using [2-(3)h]myoinositol at isotope equilibrium conditions. the following amounts were determined (nmol/mumol phospholipid, as a unit of cell mass): exponentially growing normal and tumor cells contained 25--40 nmol free inositol, 0.40--0.45 nmol myoinositol 1-phosphate, 0.30--0.50 nmol glycerylphosphoinositol, and 0.03--0.04 ...1977202462
[cytochemical study of different stages in the life cycle of toxoplasma gondii. vii. oxidation--reduction enzymes in the cyst forms].dehydrogenases of glycolysis, kreb's cycle, and pentose-phosphate shunt were detected in cystozoites of toxoplasma gondii strain ss-119 with various degrees of activity. a mixed oxidative metabolism may be postulated on this stage of the toxoplasma life cycle. besides, the activity of cytochrome oxidase was detected in cystozoites; the addition of cytochrome c to the incubation medium significantly intensified the reaction intensity. of interest seems the observation of a layer of higher enzymat ...1977203066
regulatory substances produced by lymphocytes. vi. cell cycle specificity of inhibitor of dna synthesis action in l cells.ids inhibits dna synthesis and mitosis of l cells only when present during the late g1 phase of the cell cycle, as shown with l cells synchronized by a variety of methods. this corresponds well with earlier findings that ids inhibits dna synthesis in mitogen-stimulated lymphocytes when present between 16 and 24 h after adding mitogen. in both cell types, the inhibition produced by ids appears to be totally the result of elevation of camp level. thus, inhibitors of camp phosphodiesterase work syn ...1978203648
differences in pathogenicity and antigenicity among hog cholera virus strains.using antiserum against a particular strain of bovine viral diarrhea virus, the strains of hog cholera virus were divided into two groups, h and b, on the basis of the difference in the degree of neutralization. group h consisted of strains reacting poorly in neutralization, and group b consisted of strains reacting well with bovine viral diarrhea antiserum. most of the strains of group h induced a typical clinical form of hog cholera in experimentally infected pigs. inoculation of pigs with a s ...1977203871
on the role of cellular calcium in the response of the parotid to dibutyryl and monobutyryl cyclic amp. 1978204841
[cell biological studies on the development of drug induced crystalloid inclusion bodies in the pigment epithelium of the retina of albino rats]. 1977205056
[instability of the dna content in entamoeba histolytica].dna content was repeatedly determined during a 10-month period on axenically cultured tropho-zoites from three strains (hk-9:nih, hm-2:imss and hm-3:imss) and a clone of hk-9:nih strain of entamoeba histolytica, by means of the diphenylamine reaction. average dna content was more or less variable in all of them. according to their extent, variations were: 1) large (almost four times in strain hm-2:imss), or 2) small (1.5 times in the clonal isolate). with this relatively small sample, three stat ...1978211941
comparison of interferon action in interferon resistant and sensitive l1210 cells.translation inhibition, leu-trna aminoacylation and double-stranded rna and atp dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse l 1210 r and l 1210 s cells. no differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67k dalton phosphoprotein fraction in interferon-treated l 1210 s cell-free extracts. in non-responding cell-free lysates, the lack of sti ...1978212514
difference in the effect of glucagon and starvation upon l-type pyruvate kinase from rat liver. 1978216548
outbreaks of classical swine influenza in italy in 1976. 1978217205
purification and some properties of cytochrome c553(550) isolated from desulfovibrio desulfuricans norway. 1979219858
hormonally regulated phosphoprotein of turkey erythrocytes: localization to plasma membrane.the catecholamine-stimulated cotransport of sodium and potassium ions across the plasma membrane of the turkey erythrocyte was previously found to be associated with increased 32p incorporation into a high molecular weight protein. to determine the subcellular localization of this phosphorylated protein, which we have termed goblin, a new method has been developed for isolation of pure plasma membranes from turkey erythrocytes. with this method, it has been demonstrated that goblin is located in ...1979229109
structure and sequence of the multihaem cytochrome c3.the molecular structure of cytochrome c3 isolated from desulfovibrio desulfuricans has been solved on the basis of its crystallographic determination at 2.5 a resolution and of an essentially complete sequence. the molecule consists of a single polypeptide chain wrapped around a very compact core of four non-parallel haems which present a relatively high degree of exposure to the solvent. alignment of the amino acid sequences of cytochrome c3 from several species suggests that the structure repo ...1979229424
structure-function relationship in hemoproteins: the role of cytochrome c3 in the reduction of colloidal sulfur by sulfate-reducing bacteria.cytochromes c3 of different strains of sulfate-reducing bacteria have been purified and tested for their capacity to reduce colloidal sulfur to hydrogen sulfide. the results are in good agreement with the activities reported for the whole cells. cytochrome c3 is the sulfur reductase of some strains of sulfate-reducing bacteria such as desulfovibrio desulfuricans norway 4 and sulfate-reducing bacterium strain 9974 from which the sulfur reductase activity can be purified with the cytochrome c3. in ...1979229785
pituitary stalk-section and some of its effects on endocrine function in the fetal lamb.a detailed description is given of a method to section the pituitary stalk of the fetal lamb after 105 days gestational age. the approach to the stalk is made through a window in the frontal bone. in order to prevent regeneration of the hypothalamo-pituitary connections a silicone plate is introduced through the probe used to fracture the stalk. the surgical outcome and viability of 11 pituitary stalk sectioned fetuses is described over periods of up to 23 days. the presence of pituitary infarct ...1978250110
gallium-67 citrate in human tumors. ultrastructural localization by autoradiography. 1978274621
the preparation and characterization of a cell-free system from saccharomyces cerevisiae that translates natural messenger ribonucleic acid.a cell-free protein-synthesizing system has been prepared from saccharomyces cerevisiae by differential centrifugation of lysed spheroplasts. the preparation, a modified 100,000 x g supernatant fraction, contains ribosomes and monosomes, ribosomal subunits, translation factors, and aminoacyl-trna synthetases, but no polysomes. after removal of small amounts of remaining mrna with micrococcal nuclease, protein synthesis is stringently dependent on the addition of mrna, as well as amino acids and ...1979374404
the effects of probenecid upon the individual components of indomethacin elimination.the effects of probenecid upon plasma profiles for indomethacin and upon the individual components of total plasma clearance have been evaluated in dogs, monkeys and rats. these three species cover the wide spectrum of species differences with respect to the relative contribution of renal, biliary and metabolic clearances to elimination of indomethacin. in dogs, in which biliary clearance is the major component of elimination, probenecid raised incremental values for area under the plasma curve ...1977404419
incidence and pathological features of spontaneous tumors in athymic nude mice.systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ...1979427772
neurectoderm markers retained in phenotypical skeletal muscle cells arising from a glial cell line. 1979492321
[response of certain laboratory animals to being fed an ocean fish, the ruvette]. 1979516605
study of ve virus and isolation of sle, ee, group c, and guama group arboviruses in the amazon region of peru, 1975. 1979519080
hypothalamic sensitivity to leukocytic pyrogen of adult and new-born guinea-pigs.1. experiments were conducted to localize the hypothalamic site of action of microinjected leucocytic pyrogen and to compare the pyrogenic sensitivity of this locus in adult and new-born guinea-pigs.2. to identify the site reactive to leucocytic pyrogen, bilateral (0.8-1.0 mm from the mid line) injections of 1 microliter were made into conscious adult guinea-pigs via cannulas stereotaxically palced at 0.5 mm intervals and varying depths from the olfactory tegmentum to the mammillary bodies. inje ...1979529081
transformation of mercuric chloride and methylmercury by the rumen microflora.the microflora in strained rumen fluid did not methylate or volatilize 203hg2+ at detectable rates. however, there was an exponential decay in the concentration of added ch3hg+, which was attributed to demethylation. the major product of demethylation was metallic mercury (hg0), and it was released as a volatile product from the reaction mixture. demethylation occurred under both anaerobic and aerobic conditions. the rate of demethylation was proportional to the concentration of added ch3hg+-hg ...1979539820
radioisotope assay for the quantification of sulfate-reducing bacteria in sediment and water.a radioisotope enrichment culture method was developed to estimate the physiologically active component of a population of sulfate-reducing bacteria in environmental water and sediment samples. aliquots of water or sediment were added to 50-ml serum bottles filled with 35-s-sulfate broth incubated for approximately 30 h. after incubation, the disintegration rate per milliliter of spent medium was measured, and the percentage of loss of activity resulting from bacterial sulfate reduction was dete ...1975807162
significance and taxonomic value of iso and anteiso monoenoic fatty acids and branded beta-hydroxy acids in desulfovibrio desulfuricans.the fatty acids obtained from extractable lipids of the anaerobic sulfate bacterium desulfovibrio desulfuricans were identified. saturated and monoenoic iso (c15-c19) and anteiso (c15, c17) fatty acids and saturated normal (c14-c18) and monoenoic normal (c16, c18) fatty acids were shown to be shown to be present by capillary gas chromatography-mass spectrometry. iso and anteiso beta-hydroxy fatty acids were analyzed as trimethylsilyl ethers in the same way. the position of methyl branches in the ...1977845113
anaerobic vibrio-like organisms cultured from blood: desulfovibrio desulfuricans and succinivibrio species.two unusual anaerobic vibrio-like organisms were recovered from blood cultures of two patients. one isolate was identified as desulfovibrio desulfuricans. it appeared to be the cause of a 24-h episode of fever, chills, and profuse perspiration. this is apparently the first documented report of human infection due to this organism. the second isolate was a succinivibrio species. it has rarely been described as a cause of bacteremia. the clinical significance of the organism remains unclear.1977858783
growth of desulfovibrio in lactate or ethanol media low in sulfate in association with h2-utilizing methanogenic bacteria.in the analysis of an ethanol-co(2) enrichment of bacteria from an anaerobic sewage digestor, a strain tentatively identified as desulfovibrio vulgaris and an h(2)-utilizing methanogen resembling methanobacterium formicicum were isolated, and they were shown to represent a synergistic association of two bacterial species similar to that previously found between s organism and methanobacterium strain moh isolated from methanobacillus omelianskii. in lowsulfate media, the desulfovibrio produced ac ...1977879775
influence of the concentration of phosphorus, potassium, calcium and iron compounds on the microbial reduction of sulphates.in the reduction process of sulphates by desulfovibrio desulfuricans suitable concentration of mineral salts plays an important role. in particular, different k2hpo4, cacl2 and fe(nh4)2(so4)2 content in the reaction medium has an observable effect on the reduction degree (x'), the induction time (t0) and the reduction rate constant (k). these data were recorded for 9 liquid media characterized by different content of mineral salts. the reaction rate constants were calculated for the reduction pr ...1976937089
elucidation of hydrocarbon structure in an enzyme-catalyzed benzo[a]pyrene-poly (g) covalent complex.the carcinogen, benzo[a]pyrene, was covalently attached to poly (g) by liver microsomes from rats pretreated with 3-methylcholanthrene. the complex was hydrolyzed with enzymes or base and products were isolated by sephadex chromatography. absorbance and fluorescence spectra of the products fit that of red-shifted pyrene aromatic system and suggest that metabolism has occurred at the 7-, 8-, 9-, and 10-positions of the hydrocarbon. benzanthracene or chrysene fluorescence were not observed in thes ...19761064016
fractionation of sulfur isotopes by continuous cultures of desulfovibrio desulfuricans.sulfur isotope effects observed in lactate-limited continuous cultures of desulfovibrio desulfuricans were, in general, similar to those reported for sulfate reduction by washed cells and batch cultures. there was a trend towards higher fractionation at low growth rates.19751201506
an unexplained high incidence of calcified lesions in dog kidneys. 19761265979
[differential diagnosis of ascites and abscess forming hepatitis in aids patients with reference to the first patient with microsporidia infection in austria].we report on a 30 years-old female aids patient suffering from generalized pneumocystosis and intestinal microsporidiosis. the chest x-ray showed a right-sided pleural effusion; the lungs showed no areas of consolidation and the heart and the vessels were normal in size. sonography revealed multiple cystic lesions of the liver measuring 1-3 cm in diameter, as well as ascites. pneumocystis carinii was detected on cytological examination of the bronchoalveolar lavage fluid, the pleural effusion an ...19921288019
accumulation of p53 tumor suppressor gene protein: an independent marker of prognosis in breast cancers.mutations of the tumor suppressor gene p53 have been identified in breast cancer cell lines, and some breast carcinomas are detectable by immunohistochemical assay because of p53 protein accumulation.19921317462
multiple rearrangements and activated expression of c-myc induced by woodchuck hepatitis virus integration in a primary liver tumour.woodchuck hepatitis virus (whv) is a small, partially double-stranded dna virus. like the related human hepatitis b virus (hbv), whv induces acute and chronic hepatitis and hepatocellular carcinoma (hcc) in its natural host. whv dna integration into c-myc and n-myc, resulting in deregulated expression of these genes, has been described previously in woodchuck hcc. we have analysed a woodchuck liver tumour in which whv dna was integrated in the c-myc gene. the virus insertion provoked multiple al ...19921317604
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