Publications

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Abundance, Diversity and Activity of Sulfate-Reducing Prokaryotes in Heavy Metal-Contaminated Sediment from a Salt Marsh in the Medway Estuary (UK).We investigated the diversity and activity of sulfate-reducing prokaryotes (SRP) in a 3.5-m sediment core taken from a heavy metal-contaminated site in the Medway Estuary, UK. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene ß-subunit (dsrB) and taking into account DNA extraction efficiency. This showed that SRPs were abundant throughout the core with maximum values in the top 50 cm of the sediment core making up 22.4% of the total bacterial community and ...201122124626
Characterization of the bacterial community composition in a hypoxic zone induced by Microcystis blooms in Lake Taihu, China.Cyanobacterial blooms have become more frequent as a result of eutrophication in lakes. The accumulation and breakdown of huge cyanobacterial biomasses often cause hypoxia in lakes. However, little is known about microbial diversity in these areas. In this study, we characterized the bacterial community composition of a Microcystis-bloom-induced hypoxic area in Lake Taihu, which is a large, shallow lake, by analysing terminal restriction fragment length polymorphisms of 16S ribosomal RNA genes a ...201122126440
structural and mechanistic insights into an unusual thiol disulfide oxidoreductase.cytoplasmic desulfothioredoxin (dtrx) from the anaerobe desulfovibrio vulgaris hildenborough has been identified as a new member of the thiol disulfide oxidoreductase family. the active site of dtrx contains a particular consensus sequence, cphc, never seen in the cytoplasmic thioredoxins and generally found in periplasmic oxidases. unlike canonical thioredoxins (trx), dtrx does not present any disulfide reductase activity but it presents instead an unusual disulfide isomerase activity. we have ...201122128175
Efficacy of natural biocide on control of microbial induced corrosion in oil pipelines mediated by Desulfovibrio vulgaris and Desulfovibrio gigas.We compared the efficacy of a natural biocide with four chemical tetrakishydroxymethyl phosphonium sulfonate, benzyl trimethyl ammonium chloride, and formaldehyde, glutaraldehyde, to control microbial induced corrosion in oil pipelines. The efficacy of biocides were monitored against Desulfovibrio vulgaris and Desulfovibrio gigas in experimental pipes by measuring cell counts, H2S production, Fe(II) production, production of extracellular polymeric substances and structure of biofilm. The treatm ...201122128548
comparative electrochemical study of superoxide reductases.superoxide reductases are involved in relevant biological electron transfer reactions related to protection against oxidative stress caused by reactive oxygen species. the electrochemical features of metalloproteins belonging to the three different classes of enzymes were studied by potentio-dynamic techniques (cyclic and square wave voltammetry): desulfoferrodoxin from desulfovibrio vulgaris hildenborough, class i superoxide reductases and neelaredoxin from desulfovibrio gigas and treponema pal ...201122143105
functional characterization of crp/fnr-type global transcriptional regulators in desulfovibrio vulgaris hildenborough.crp/fnr-type global transcriptional regulators regulate various metabolic pathways in bacteria and typically function in response to environmental changes. however, little is known about the function of four annotated crp/fnr homologs (dvu0379, dvu2097, dvu2547 and dvu3111) in desulfovibrio vulgaris hildenborough. a systematic study using bioinformatic, transcriptomic, genetic and physiological approaches was conducted to characterize their roles in stress responses. similar growth phenotypes we ...201122156435
comparative 16s rrna gene surveys of granular sludge from three upflow anaerobic bioreactors treating purified terephthalic acid (pta) wastewater.the microbial communities from three upflow anaerobic bioreactors treating purified terephthalic acid (pta) wastewater were characterized with 16s ribosomal rna gene sequencing surveys. universal bacterial and archaeal primers were used to compare the bioreactor communities to each other. a total of 1,733 bacterial sequences and 383 archaeal sequences were characterized. the high number of syntrophus spp. and pelotomaculum spp. found within these reactors indicates efficient removal of benzoate ...201122179636
identifying fermenting bacteria in anoxic tidal-flat sediments by a combination of microcalorimetry and ribosome-based stable-isotope probing.a novel approach was developed to follow the successive utilization of organic carbon under anoxic conditions by microcalorimetry, chemical analyses of fermentation products and stable-isotope probing (sip). the fermentation of (13) c-labeled glucose was monitored over 4 weeks by microcalorimetry in a stimulation experiment with tidal-flat sediments. based on characteristic heat production phases, time points were selected for quantifying fermentation products and identifying substrate-assimilat ...201122188432
Microbiology of regressive autism.This manuscript summarizes some of our earlier work on the microbiology of autism subjects' stool specimens, as compared with stools from control subjects. Our most recent data indicating that Desulfovibrio may play an important role in regressive autism is also presented. In addition, we present information on antimicrobial susceptibility patterns of Desulfovibrio using the CLSI agar dilution susceptibility technique. In addition, we summarize data from our earlier studies showing the impact of ...201122202440
Lactobacillus salivarius REN counteracted unfavorable 4-nitroquinoline-1-oxide-induced changes in colonic microflora of rats.Probiotics and carcinogens both have a significant effect on the microfloral composition of the human intestine. The objective of this study was to investigate the impact of an important carcinogen, 4-Nitroquinoline-1-Oxide on colonic microflora and the efficacy of the probiotic Lactobacillus salivarius REN as an agent of counteracting these effects. Using denaturing gradient gel electrophoresis (DGGE) combined with redundancy analysis, we demonstrated that both 4-Nitroquinoline-1-Oxide and L. s ...201122203549
using gas chromatography/isotope ratio mass spectrometry to determine the fractionation factor for h(2) production by hydrogenases.hydrogenases catalyze the reversible formation of h(2), and they are key enzymes in the biological cycling of h(2). h isotopes have the potential to be a very useful tool in quantifying hydrogen ion trafficking in biological h(2) production processes, but there are several obstacles that have thus far limited the application of this tool. here, we describe a new method that overcomes some of these barriers and is specifically designed to measure isotopic fractionation during enzyme-catalyzed h(2 ...201222215579
microbial community succession during lactate amendment and electron-acceptor limitation reveals a predominance of metal-reducing pelosinus spp.determining the success of in-situ bioremediation strategies is complex. by using controlled laboratory conditions, the influence of individual variables such as u(vi), cr(vi) and electron donor and acceptors on community structure, dynamics, and the metal-reducing potential can be studied. triplicate anaerobic, continuous-flow reactors were inoculated with cr(vi) contaminated groundwater from the hanford, washington 100-h area, amended with lactate and incubated for 95 days to obtain stable, en ...201222267668
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