[fusobacterium necrophorum septicemia]. 19948066062
restriction fragment length polymorphism analysis of fusobacterium necrophorum using a novel repeat dna sequence and a 16s rrna gene probe.a repeated dna sequence was isolated from fusobacterium necrophorum biotype ab, strain fns1. the repeated sequence shared considerable homology with the transposase gene from the pseudomonas syringiae insertion sequence is801. the repeat sequence was used together with a 16s ribosomal rna gene probe to type f. necrophorum isolates using restriction fragment length polymorphisms. the probes revealed differences between several clinical isolates and will be useful tools to study the epidemiology o ...19938097174
fusobacterium necrophorum septicemia complicated by liver abscess. a case report.a previously healthy 27-year-old man developed fusobacterium necrophorum septicemia complicated by a solitary liver abscess. this is an unusual course of necrobacillosis, in addition to fusobacterium necrophorum being a rare cause of anaerobic liver abscesses. the patient was treated with percutaneous drainage and a prolonged course of ampicillin and metronidazole therapy. he had a successful recovery.19938110445
[lemierre's syndrome. case report].the paper deals with a thrombophlebitis of the internal jugular vein, coming after a plaut-vincent's angina, associated to septic embolism of the lung. the syndrome was first described by lemierre, in 1936. together with a survey of the cases reported in the last ten years is made.19938135336
[diagnostic imaging in lemierre syndrome]. 19938142516
characterization, distribution, and microbiological associations of fusobacterium spp. in clinical specimens of animal origin.during the years from 1984 through 1991, 1,067 specimens from canine, equine, exotic, feline, porcine, and ruminant animal sources were found to contain members of the genus fusobacterium: the most common sites or conditions from which members of this genus were isolated were abscesses, the respiratory tract, and pleural and peritoneal cavities. most specimens contained a single fusobacterium species. the most commonly isolated species was fusobacterium necrophorum. almost all of the specimens c ...19948150946
serum neutralizing antibodies against fusobacterium necrophorum leukotoxin in cattle with experimentally induced or naturally developed hepatic abscesses.the relationship between serum-neutralizing antibody against fusobacterium necrophorum leukotoxin and hepatic abscesses was investigated in cattle fed diets supplemented with or without tylosin. sixteen cattle (eight each in tylosin and in control groups) were inoculated intraportally with f. necrophorum. ultrasonographic scanning showed that all control animals developed hepatic abscesses after inoculation. in the tylosin group, two animals were free of abscess by d 7 and one was free by d 14. ...19948157537
phylogenetic relationship of fusobacterium necrophorum a, ab, and b biotypes based upon 16s rrna gene sequence analysis.the 16s rrna genes from virulent (ab) and benign (b) biotype strains of fusobacterium necrophorum, the causative agent of ovine foot abscess, were cloned and sequenced. notwithstanding the distinct phenotypic differences between the ab and b biotypes, a phylogenetic analysis in which the 16s rrna gene sequences were used revealed the close relationship of these taxa. comparison of the virulent and benign biotypes of f. necrophorum may therefore be a legitimate way to identify key virulence facto ...19948186096
delayed-type hypersensitivity in mice experimentally infected with fusobacterium necrophorum.the foot-pad test was performed to reveal the induction of delayed-type hypersensitivity (dth) to fusobacterium necrophorum in mice experimentally infected with f. necrophorum. for the foot-pad test, heat-killed cells of f. necrophorum were found to be better than viable bacteria as eliciting antigens. the thickness of foot-pad could be measured properly at 48 hr after administration to the foot-pad of heat-killed bacteria. an intraperitoneal dose of the order of 10(5) or 10(6) of viable bacteri ...19948204761
three cases of fusobacterium necrophorum septicemia.two cases of necrobacillosis or post-anginal septicemia (lemierre's syndrome) due to fusobacterium necrophorum are described. the first case in a young adult was complicated by thrombophlebitis of the internal jugular vein, lung abscesses and skin involvement. the second case was in a 2-year-old child, which is exceptional. the primary focus was possibly otitis media. in a third patient with multiple metastases of colon carcinoma, fusobacterium necrophorum septicemia was diagnosed during a bout ...19938243489
lemierre's syndrome: two cases of a forgotten disease. 19948263648
[post-pharyngitis sepsis caused by fusobacterium necrophorum: lemierre syndrome]. 19938264841
fusobacterium septic arthritis of the sternoclavicular joint.we describe a case of septic arthritis of the sternoclavicular joint caused by fusobacterium in an otherwise healthy young man. the fusobacterium had morphologic features most in keeping with fusobacterium (f.) necrophorum, which was a common organism in sternoclavicular septic arthritis in the preantibiotic era. to our knowledge this is the first reported case of a fusobacterium sternoclavicular joint infection in the antibiotic era. this case should lead to heightened awareness of sternoclavic ...19938308792
[acute mastoiditis due to anaerobic bacteria. review of the literature apropos of a case].the authors report a case of acute mastoiditis in a four year-old boy due to fusobacterium necrophorum. the importance of the complications (septiciemia, lateral sinus thrombosis, meningitis, osteitis) and the fusobacterium necrophorum are the two majors interests of this observation. a review of the literature is performed.19938317866
lemierre's syndrome caused by fusobacterium necrophorum: a case report. 19938320498
[diagnostic imaging in lemierre's+ syndrome].the lemierre syndrome is a septic picture with jugular thrombophlebitis and secondary septic embolisms usually produced by fusobacterium necrophorum. in previous reports published in the literature, imaging techniques are seldom referred to report the presence of jugular thrombophlebitis. a case of the lemierre syndrome is presented with the aim of calling attention to this process and its current diagnostic possibilities.19938324024
bacteriologic findings in peritonsillar abscesses in young adults.aspirated pus samples from 124 patients with peritonsillar abscess were cultured quantitatively for aerobes and anaerobes. a total of 98% of the samples yielded bacteria. of the 550 isolates obtained (mean, 4.4 per patient), 143 were aerobes (representing 16 species or groups) and 407 were anaerobes (representing 40 species or groups). aerobes were isolated from 86% of patients-alone in 20 cases and together with anaerobes in 87. the most common aerobic isolates were streptococcus pyogenes (isol ...19938324134
multiple brain abscesses and bacteremia in a child due to fusobacterium necrophorum.a case of fusobacterium necrophorum bacteremia and multiple brain abscesses in a 6-year-old child following a mycoplasma pneumoniae infection is described. brain abscess due to this microorganism has only been described in three other cases since 1977. the clinical picture was consistent with postanginal septicemia, with septic metastatic complications normally only seen in teenagers and young adults. the patient was successfully treated with a 2-month course of parenteral penicillin g and metro ...19938365815
partial characterization of hcl heat extracted antigen derived from fusobacterium necrophorum.the hcl heat extracted antigen derived from fusobacterium necrophorum was partially purified by cm cellulose column chromatography and treatment by freeze-thawing. it demonstrated relatively high enzyme-linked immunosorbent assay values to anti-f. necrophorum sera. in sodium dodecyl sulphate polyacrylamide gel electrophoresis, the partially purified preparation exhibited one definite and two faint bands. the approximate molecular weight of the former was estimated as 16 kd.19938366827
[post-pharyngitis sepsis caused by fusobacterium necrophorum: lemierre's syndrome]. 19938366943
comparison of extracellular enzymes of fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme.a total of 10 strains each of fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme were tested for the production of 13 extracellular enzymes. dnase, alkaline phosphatase, and lipase were predominantly associated with all the strains of f. necrophorum subsp. necrophorum, with dnase not detected in any of the strains of f. necrophorum subsp. funduliforme. in addition, the strains of f. necrophorum subsp. necrophorum were generally more hemolytic than thos ...19938370761
fusobacteria: new taxonomy and related diseases.fusobacteria are anaerobic gram-negative bacilli. since the first reports in the late nineteenth century, various names have been applied to these organisms, sometimes with the same name being applied to different species. more recently, not only have there been changes to the nomenclature, but also attempts to differentiate between species which are believed to be either pathogenic or commensal or both. because of their asaccharolytic nature, and a general paucity of positive results in routine ...19938411084
lemierre syndrome: the forgotten disease.lemierre syndrome is characterized by an acute oropharyngeal infection, suppurative thrombophlebitis of the internal jugular vein, anaerobic sepsis, and metastatic infections. before the discovery of antibiotics, this disease usually was fatal. we report the case of a patient with all of these findings, indicating that lemierre syndrome still is seen today. prompt recognition, abscess drainage, and appropriate antibiotic coverage result in complete recovery in most patients.19938427443
meningitis due to fusobacterium necrophorum subspecies necrophorum. case report and review of the literature.this paper reports a case of fatal meningitis caused by fusobacterium necrophorum subsp. necrophorum in a previously healthy five-year-old child. the organism was isolated in pure culture from the cerebrospinal fluid and from intracranial pus obtained at autopsy. the role of f. necrophorum in the development of meningitis is reaffirmed and its isolation and identification are discussed. the clinical presentation of the present case resembles the previously published reports and highlights the po ...19938449585
structural studies of the o-antigenic polysaccharide of fusobacterium necrophorum.the o-specific polysaccharide component of the lipopolysaccharide produced by fusobacterium necrophorum is of the teichoic acid type, with repeating units connected by phosphoric diester linkages. dephosphorylation of the polysaccharide by treatment with aqueous hydrogen fluoride yielded a carbohydrate composed of a trisaccharide linked to an acidic component. this product, and the polysaccharide, were investigated by chemical methods and 1h-, 13c-, 31p- and 15n-nmr spectroscopy and the former a ...19938462551
the prevalence of fusobacterium necrophorum biovar a in animal faeces.only a small proportion of animals tested were found to be excreting fusobacterium necrophorum biovar a, the causative organism of necrobacillosis, in the faeces (3 of 69 wallabies, 1 of 66 deer, 2 of 81 cattle). the two positive cattle belonged to a single group of calves on a farm with a history of necrobacillosis and the litter underfoot also readily yielded biovar a organisms. all attempts to demonstrate biovar a in litter on other farms and in soil from an area populated by wallabies and de ...19938472776
effect of disturbance of the gastrointestinal microflora on the faecal excretion of fusobacterium necrophorum biovar a.oral pretreatment of mice with either a mixture of kanamycin and erythromycin or metronidazole to modify the gut microflora greatly enhanced the faecal excretion of fusobacterium necrophorum biovar a given by mouth. this lends support to the suggestion that disturbance of the gastrointestinal microflora in animals such as cattle, which often carry the organism in the rumen, may lead to intestinal multiplication and faecal excretion, thereby providing a source of infection that may lead to necrob ...19938472777
adult respiratory distress syndrome as a complication of postanginal respiratory distress syndrome (ards) was described in 1971 by petty and ashbaugh. since that time it has been reported in association with many disease entities. we report a case in which a patient with postanginal sepsis, also known as lemierre's syndrome, had development of ards.19938486067
[postanginal sepsis caused by fusobacterium necrophorum: lemierre syndrome].postanginal sepsis or lemierre's syndrome is characterised by septic thrombophlebitis of the jugular vein, metastatic abscesses in the lungs, soft tissues, joints or elsewhere, occurring several days to two weeks after tonsillitis or pharyngitis. the primary pathogen is a gram-negative anaerobic rod, mostly fusobacterium necrophorum. previously healthy, young adults are affected mainly and the syndrome was seen more frequently in the pre-antibiotic era than it is nowadays. in the three young pat ...19938505987
outer membrane proteins of fusobacterium necrophorum biovars a, ab and b: their taxonomic relationship to f. necrophorum subspecies necrophorum and f. necrophorum subspecies funduliforme. 19938507709
pathogenicity of fusobacterium necrophorum strains from man and animals.necrobacillosis occurs in man and animals. the typical forms of the disease in animals are caused by fusobacterium necrophorum biovar a; biovar b strains are much less pathogenic. in this study the pathogenicity for mice of eight human isolates of f. necrophorum was compared with that of animal biovar a and b strains. by subcutaneous inoculation seven of the human strains differed from biovar a but resembled biovar b in (1) producing, at the most, mild local lesions that rapidly healed, and (2) ...19938519315
necrobacillosis: are we missing the early stages of this life-threatening infection? 19958551842
unusual radiological manifestations of lemierre's syndrome: a case report.lemierre's syndrome is an uncommon clinical entity characterized by oropharyngeal infection followed by septic thrombophlebitis of the jugular vein with embolization to the lungs and other organs. the organism is a gram-negative anaerobic bacterium, fusobacterium necrophorum. we report a case of lemierre's syndrome in an 8-year-old child who presented with septic arthritis of the left hip joint. roentgenograms and computed tomography demonstrated gas in the joint and adjacent soft tissues, along ...19958577497
lemierre's syndrome: a case of postanginal septicemia and bilateral flank abscesses.lemierre's syndrome is characterized by pharyngeal infections in young healthy adults with secondary septic thrombophlebitis and multiple metastatic infections. in the preantibiotic era, lemierre's syndrome was common and lethal. with the advent of antibiotics, lemierre's syndrome has become such a rare entity that the diagnosis is often delayed or missed. with prompt recognition, appropriate antibiotic therapy, and surgical drainage of metastatic abscesses, the majority of patients can be cured ...19958579274
jugular thrombophlebitis complicating bacterial pharyngitis (lemierre's syndrome).lemierre s syndrome is a rare syndrome caused by fusobacterium necrophorum, a gram-negative anaerobic organism that normally inhabits the oropharynx. the syndrome follows primary oropharyngeal infection and affects previously healthy adolescents in a characteristic manner with fatal results if left untreated. the authors present two cases seen at their institution and discuss the clinical and radiologic features of the syndrome, along with considerations for patient management.19968587815
ribotyping to differentiate fusobacterium necrophorum subsp. necrophorum and f. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. hemagglutination activity was present in all hepatic, but only some ruminal, strains of fusobacterium necrophorum subsp. necrophorum. ruminal f. necrophorum subsp. necrophorum had low leukotoxin titers yet was virulent in mice. fusobacterium necrophorum subsp. funduliforme ...19968593050
microbiological features and pathogenesis of peritonsillar abscesses.samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ...19958605900
[lemierre's post-tonsillitis sepsis with meningitis and intravascular consumption coagulopathy as complication of infectious mononucleosis with pansinusitis].24 days after the onset of infectious mononucleosis, clinically and serologically confirmed, an otherwise healthy 18-year-old schoolboy developed a fulminant septicaemia with acute meningitis and loss of consciousness, consumptive coagulopathy and acute renal failure.19968631245
peritoneal interleukin-8 in acute appendicitis.interleukin-8 (il-8) is a chemoattractant that is highly selective for neutrophils. this study was designed to investigate the presence of il-8 in peritoneal fluid of patients with acute appendicitis. the clinical circumstances underlying the secretion of il-8 by mesothelium and its mechanism of activation have not been defined. in an in vitro model for bacterial peritonitis the role of bacteria in activating human mesothelial cells to secrete il-8 was studied. cultured human mesothelium was inc ...19968632651
clinical significance of dental root canal microflora.previous work by this group has shown that a significant association exists between pain and the presence of either prevotella or peptostreptococcus spp. in dental root canals. the aim of this study was to examine a more extensive series of canals microbiologically, to determine whether any other particular endodontic symptoms or clinical signs showed specific associations with individual bacterial species.19968636492
[fever and abdominal pain in a 17-year-old patient]. 19968637297
comparison of haemolytic activity between fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme in vitro and in vivo.haemolytic activity of two subspecies of fusobacterium necrophorum was compared in vitro and in vivo. f. necrophorum subsp. necrophorum (fnn) showed a stronger activity than f. necrophorum subsp. funduliforme (fnf) in vitro. haemolytic activity of fnn and fnf was 57.97%+/-1.90 and 17.33%+/-1.44, respectively, compared to complete haemolysis by distilled water. in the mice injected with fnn, haemolysin was detected in the liver at a titre of from 1 : 16 to 1 : 128, and fnn was recovered from all ...19968645462
a case of fusobacterium necrophorum sepsis. 19958666847
an experimental ovine foot abscess model using a fusobacterium necrophorum biotype experimental procedure is described for the production of foot abscess in sheep that mimics the natural disease. lesions were produced by the intradermal inoculation of suspensions of fusobacterium necrophorum biotype ab containing from 5 x 10(2) to 5 x 10(8) bacteria, into interdigital skin devitalized by freezing with liquid nitrogen. a dose of 5 x 10(5) bacteria induced the development of foot abscess in 3 of 4 and 8 of 8 inoculated feet. it was found that to produce foot abscess in devita ...19968701565
fusobacterium necrophorum infections: virulence factors, pathogenic mechanism and control measures.fusobacterium necrophorum, a gram-negative, non-spore-forming anaerobe, is a normal inhabitant of the alimentary tract of animals and humans. two types of f. necrophorum, subspecies necrophorum (biotype a) and funduliforme (biotype b), have been recognized, which differ morphologically, biochemically, and biologically. the organism is an opportunistic pathogen that causes numerous necrotic conditions (necrobacillosis) such as bovine hepatic abscesses, ruminant foot abscesses and human oral infec ...19968711893
serum neutralizing antibody response and protection against experimentally induced liver abscesses in steers vaccinated with fusobacterium determine the efficacy of leukotoxin-based fusobacterium necrophorum vaccines and dietary tylosin in providing protection against experimentally induced hepatic abscesses in steers.19968712511
evidence for phospholipase b activity in fusobacterium necrophorum cultures and its association with hemolysin/leucocidin activities.phospholipase b (plb) activity was present in fusobacterium necrophorum cultures and it correlated closely with virulence and co-purified with the hemolysin/leucocidin activities. all three activities were associated with a large molecule or molecular complex (6 x 10(2)-2 x 10(3) kda) exhibiting varying degrees of aggregation. these were present mainly in the culture medium and to a lesser extent in cell sonicates. the plb and toxin activities were sensitive to heat, dissociating agents, proteol ...19968734639
necrobacillosis: an unusual cause of purulent otitis media and sepsis.necrobacillosis is a rare systemic illness caused by the anaerobic gram-negative bacterium, fusobacterium necrophorum. we describe a case of necrobacillosis in a previously healthy child who developed purulent otitis media with sepsis caused by f. necrophorum. this case demonstrates that not all cases of purulent ear drainage are caused by the usual otopathic organisms and emphasizes the importance of culturing ear discharge in a child with an unusual presentation.19958751176
fusobacterium osteomyelitis and pyarthrosis: a classic case of lemierre's syndrome.lemierre's syndrome, a systemic anaerobic infection caused by fusobacterium necrophorum, is characterized by an acute oropharyngeal infection, septic thrombophlebitis of the internal jugular vein, sepsis, and multiple metastatic infections. it commonly leads to septic arthritis and occasionally to osteomyelitis. in the preantibiotic era, this infection was nearly universally fatal. today it still poses a potentially grave threat to the young patients affected. prompt recognition with appropriate ...19968784713
susceptibilities of bovine summer mastitis bacteria to antimicrobial agents.the susceptibility to 9 antimicrobial agents of 32 aerobic bacterial isolates and to 10 antimicrobial agents of 37 anaerobic bacterial isolates from 23 cases of bovine summer mastitis (16 actinomyces pyogenes isolates, 8 streptococcus dysgalactiae isolates, 3 s. uberis isolates, 3 s. acidominimus isolates, 2 streptococcus spp., 15 peptostreptococcus indolicus isolates, 10 fusobacterium necrophorum isolates, and 12 isolates of anaerobic gram-negative rods) was determined by the agar dilution meth ...19968787898
lemierre's syndrome: are we underdiagnosing this life-threatening infection?over a period of 12 months 3 patients presented with severe tonsillitis and subsequently grew fusobacterium necrophorum (literally-spindle-shaped rod which brings death!) from blood cultures. two patients responded well to antibiotics and were discharged within 4 days of admission. the third case had a longer history of symptoms and following investigations of a minor but unusual neck swelling was found to have thrombosis of the external and internal jugular vein. this is the first stage in the ...19968797225
stability and stabilization of fusobacterium necrophorum hemolysin.the stability and stabilization of the hemolytic activity of fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme were monitored over a period of four weeks using culture supernatants. the hemolytic activity was completely lost after one week at room temperature and 37 degrees c. after a two-week storage at 4 degrees c and -80 degrees c only trace activity was detected with -80 degrees c being the better of the two conditions. the addition of cysteine mo ...19968810016
the use of an experimental metritis model to study antibiotic distribution in genital tract secretions in the ewe.the influence of experimentally-induced metritis on spiramycin disposition in genital secretions was investigated in six ovariectomized ewes. a crossover study design was selected to compare control with metritis pharmacokinetics. a clinically-relevant metritis was obtained under progestagen priming by inoculation in the uterine lumen of a bacterial suspension of actinomyces pyogenes and fusobacterium necrophorum ewes were given a single iv administration of spiramycin at a dose of 20 p ...19968822617
the effects of physical and chemical agents on the secretion and stability of a fusobacterium necrophorum hemolysin.the effect of various agents as enhancers or inhibitors of hemolysin secretion by fusobacterium necrophorum was investigated. the hemolysin secreted in phosphate buffered saline (pbs) alone was inactivated shortly after secretion. tween-80 or albumin preserved the hemolytic activity in pbs in which cultures of f. necrophorum had been suspended. hemoglobin was found to enhance hemolysin secretion. however, higher concentrations diminished secretion. chloramphenicol, an inhibitor of protein synthe ...19968828128
acute phase response in heifers with experimentally induced mastitis.ten pregnant heifers were inoculated in both hind udder quarters with a mixture of actinomyces pyogenes, fusobacterium necrophorum and peptostreptococcus indolicus. development of the experimental mastitis was monitored by sequential clinical and bacteriological examinations, and by blood acute phase protein profiles. sequential changes in plasma fibrinogen, serum haptoglobin, acid-soluble glyco-proteins and alpha 1-proteinase inhibitor activity were analysed and compared with both the clinical ...19968864931
lemierre syndrome.we report a case of lemierre syndrome. although it is seen infrequently, it must be considered in patients with sore throat or dental pain, lateral neck pain, sepsis, and pulmonary symptoms. diagnosis is based on clinical presentation, occurrence of anaerobic septicemia, radiologic evidence of internal jugular venous thrombosis, and pulmonary septic emboli.19968865803
the effect of cyclosporin-a on the oral microflora at gingival sulcus of the ferret.the effect of cyclosporin-a (cya) on the dentogingival flora of ferrets with healthy and experimentally induced periodontal breakdown was studied. five animals were given 10 mg/kg/d cya. at the start of the experiments (day 0), ligatures were placed around 4 teeth in the right upper and lower jaws; corresponding contralateral teeth on the left side served as control. on days 0 and 28 (end of the experiment), microbiological samples were collected from the gingival sulcus of the experimental and ...19968891937
fusobacterium necrophorum abortion in a cow. 19968893490
the serum neutralizing antibody response in cattle to fusobacterium necrophorum leukotoxoid and possible protection against experimentally induced hepatic abscesses.the serum antileukotoxin antibody response and protection against subsequent experimental challenge with fusobacterium necrophorum were investigated in 30 steers vaccinated with crude f. necrophorum leukotoxoid. culture supernatant of f. necrophorum, strain 25, containing leukotoxoid was concentrated. the steers were assigned randomly to six groups (n = 5): pbs control with stimulon adjuvant; vaccinated with concentrated supernatant diluted to provide 2.5, 5.0, 10.0, or 20.0 ml with the water-so ...19968950830
[multiple liver abscesses in long-term intrauterine device].we report on two cases of multiple hepatic abscesses caused by sever pelvic inflammatory disease secondary to intrauterine device. such complication have previously been reported only twice. the course of the diseases were non-specifically for a long time. as causative agent we isolated klebsiella pneumoniae in the first case and in the other fusobacterium necroforum. the patients were cured with combined operative and medical therapy.19968992822
intrauterine bacterial findings and hormonal profiles in post-partum cows with normal puerperium.the post-partum intrauterine bacterial flora, prostaglandin release, uterine involution and resumption of ovarian activity were studied in 9 swedish dairy cows during the first 8-week period. uterine involution was monitored by transrectal examinations of the reproductive tract 3 times weekly. bacteriological examination was performed from twice weekly uterine biopsies. the main pgf2 alpha metabolite (15-ketodihydro-pgf2 alpha) was monitored from twice daily blood plasma samples, while morning s ...19968996871
minimum inhibitory concentrations for selected antimicrobial agents against fusobacterium necrophorum isolated from hepatic abscesses in cattle and sheep.minimum inhibitory concentrations for 35 antimicrobial agents against 100 fusobacterium necrophorum isolates from hepatic abscesses in sheep and cattle were determined. twelve of the thirteen beta-lactam antibiotics tested inhibited growth of 100% of strains tested. of the remaining antimicrobial agents, extensive susceptibility was found for: spiramycin, josamycin, lincomycin, tylosin, oxytetracycline, chlortetracycline, rufloxacin, metronidazole, cotrimoxazole, sulfadimethoxine, virginiamycin ...19979049945
fusobacterium necrophorum meningitis associated with cerebral vessel thrombosis. 19979076827
use of enzyme-linked-immunosorbent assay for detection of igg and igm antibodies to fusobacterium necrophorum in enzyme-linked-immunosorbent assay (elisa) with hcl heat-extracted antigen of fusobacterium necrophorum was conducted to detect specific immunoglobulins g and m in infected cattle. the elisa revealed an increase (> 0.40) in specific igg in most of the animals with hepatic abscesses but not that in specific igm. all the lesions were positive for f. necrophorum. these findings indicated that the elisa for immunoglobulin g detection may prove to be a useful tool for predictive serodiagnosis of f. ...19969082147
fusobacterium necrophorum septicemia in a lamb: pathologic and microbiologic characterization. 19979087931
[lemierre's syndrome: a non-forgotten disease]. 19979091111
effect of fusobacterium necrophorum leukotoxoid vaccine on susceptibility to experimentally induced liver abscesses in cattle.the efficacy and the optimum dose of fusobacterium necrophorum crude leukotoxoid vaccine required to immunize and protect steers against experimentally induced liver abscesses were evaluated. the vaccine consisted of cell-free culture supernatant of a high leukotoxin-producing strain of f. necrophorum, inactivated with formalin and homogenized with an adjuvant. twenty-five steers were assigned randomly to the following five treatment groups: control; three doses (1.0, 2.0, and 5.0 ml) of the cul ...19979110232
polymicrobial brain abscess in a patient infected with human immunodeficiency virus.although intracranial mass lesions that occur as a result of infection have commonly been reported in patients infected with human immunodeficiency virus, polymicrobial pyogenic brain abscess has not been described in this setting. we report the first case of a patient with a polymicrobial brain abscess involving streptococcus bovis, fusobacterium necrophorum, peptostreptococcus, and group g streptococcus, and we review the relevant world literature.19979114198
penicillin g and penicillin g-tinidazole treatment of experimentally induced summer mastitis--effect on elimination rates of bacteria and outcome of the disease.two udder hind quarters of ten pregnant heifers were inoculated experimentally with a combination of actinomyces pyogenes, peptostreptococcus indolicus and fusobacterium necrophorum at a dose of 9 x 10(8) colony forming units (cfu) of each species of bacteria. the development of aerobic-anaerobic mastitis was followed-up clinically and through analysis of udder secretion samples. all heifers developed acute clinical mastitis. thick, purulent secretions with foul odour were already present 24 h a ...19949133056
possible lethal enhancement of toxins from putative periodontopathogens by nicotine: implications for periodontal test the hypothesis that lethal synergy in the chick embryo model may occur between nicotine and bacterial products (cell-free extracellular toxins and cell lysates) of five putative periodontopathogens.19979155677
interactions between fusobacterium necrophorum hemolysin, erythrocytes and erythrocyte membranes.the interactions between the hemolysin of fusobacterium necrophorum subsp. necrophorum, erythrocytes and erythrocyte membranes were studied as an attempt to determine the initial characteristics leading to hemolysis. the spectrum of erythrocyte sensitivity indicated that horse, dog and mouse erythrocytes were highly sensitive whereas those of cattle, sheep, goat and chicken were insensitive to the hemolysin. binding of hemolysin to horse and dog erythrocytes or their ghosts was more pronounced t ...19979163913
immunohistochemical localization of alpha 1-acid glycoprotein in liver tissues of bovine fetuses, newborn calves, and sick or healthy adult detect localization of alpha 1-acid glycoprotein (alpha 1-ag) antigens in the liver tissue of cattle by use of immunoperoxidase technique.19979215447
summer mastitis experimentally induced by hydrotaea irritans exposed to bacteria.summer mastitis is an acute suppurative bacterial infection of the udder in heifers and dry cows. to ascertain the possible role of flies in the transmission of the disease, experimental exposures of recipient heifers to hydrotaea irritans previously exposed to bacteria were carried out. flies were allowed to feed on secretions from clinical cases of summer mastitis. the pathogens present were the bacteria staphylococcus aureus, streptococcus dysgalactiae, actinomyces pyogenes, stuart-schwan coc ...19979226651
fever, sore throat, and pulmonary infiltrates in a 20-year-old man. 19979228388
lemierre's syndrome in children: high-resolution ct and color doppler sonography patterns.lemierre's syndrome is an anaerobic sepsis occurring after oropharyngeal infection in healthy teenagers and young adults. we report two cases of adolescent girls suffering from lemierre's syndrome studied with cervical color doppler ultrasonography (cdus), cervicothoracic helical ct, and high-resolution ct (hrct) scanning. in both patients, hrct allowed a good depiction of multiple cavitated pulmonary nodules of various sizes suggestive of this entity and was able to detect small or peripheral n ...19979228389
virulent foot rot in sheep. 19979234556
[a young man with sore throat and infection].postanginal septicemia (lemierre's syndrome) is an infection with anaerobes that ensues from certain oropharyngeal infections: septic thrombophlebitis of the internal jugular vein leads to abscess formation in the lungs and possibly in other organs. based upon a recently observed typical case the syndrome is presented and its possible importance for empirical therapy of tonsillopharyngitis and septicemias involving unknown organisms is briefly discussed.19979289812
a young woman with a sore throat, septicaemia, and respiratory failure. 19979314872
a comparative study of fusobacterium necrophorum strains from human and animal sources by phenotypic reactions, pyrolysis mass spectrometry and sds-page.fusobacterium necrophorum strains from human infection (21) were compared with strains from animals (17 biotype a, 2 biotype ab, 4 biotype b, 1 biotype unknown), and the type strain nctc 10575 in conventional tests reaction patterns (ctrps), sds-page and pyrolysis mass spectrometry (pms). classifications from the three approaches showed one major consensus group comprising all human strains, and another comprising animal biotype a strains. animal biotype b strains and one animal strain, designat ...19979364143
phenotypic characteristics and lipopolysaccharides of human and animal isolates of fusobacterium part of a collaborative study, six culture collection isolates and 50 coded isolates of fusobacterium necrophorum were examined for the types of lipopolysaccharides (lps) they contained, and to see if this related to their reactions in a range of phenotypic tests and their susceptibility to a panel of six antimicrobial compounds. the biotype b type strain, putative biotype b isolates and human isolates were predominantly coccobacillary, had rough type lps and some of these isolates (8 of 26) ...19979364144
classification of human and animal strains of fusobacterium necrophorum by their pathogenic effects in mice.forty-six strains of fusobacterium necrophorum, 24 from animals and 22 of human origin, were examined by pathogenicity tests in mice, while the same strains were being examined in laboratories elsewhere by other methods. the pathogenicity tests consisted of (1) subcutaneous inoculation with a large dose of a pure culture, (2) subcutaneous inoculation with a small dose of f. necrophorum mixed with a large but relatively harmless dose of staphylococcus aureus, and (3) intravenous inoculation with ...19979364145
septic arthritis of the hip by fusobacterium necrophorum after tonsillectomy: a form of lemierre syndrome?lemierre syndrome used to be a complication of severe oropharyngeal infection with regional thrombophlebitis, septicaemia and septic metastatic infections caused by fusobacterium necrophorum in the pre-antibiotic era. a case of septic arthritis of the hip caused by f. necrophorum as a complication of tonsillectomy is reported in a 9-year-old boy. conclusion: lemierre syndrome, usually seen after an oropharyngeal infection, can also complicate tonsillectomy.19979392400
ribotyping to compare fusobacterium necrophorum isolates from bovine liver abscesses, ruminal walls, and ruminal contents.restriction fragment length polymorphism analysis of rrna genes was employed to genetically compare fusobacterium necrophorum subsp. necrophorum and f. necrophorum subsp. funduliforme isolates from multiple abscesses of the same liver and isolates from liver abscesses, the ruminal wall, and ruminal contents from the same animal. four livers with multiple abscesses and samples of ruminal contents, ruminal walls, and liver abscesses were collected from 11 cattle at slaughter. f. necrophorum was is ...19979406386
septic arthritis of the ankle from fusobacterium necrophorum. 19979408435
lemierre's syndrome: new insights into an old disease. 19979415841
antimicrobial susceptibility of fusobacterium necrophorum isolated from bovine hepatic determine the resistance and susceptibility to antimicrobial compounds of fusobacterium necrophorum isolates from bovine hepatic abscesses.19989442241
endopeptidase activities of selected porphyromonas spp., prevotella spp. and fusobacterium spp. of oral and non-oral origin.the ability of three porphyromonas spp., seven prevotella spp., seven fusobacterium spp. and two related bacteroides spp. (b. levii and b. macacae) to degrade an extensive range of synthetic endo-, amino- and diamino peptidase substrates linked to the fluorescent leaving group 7-amido-4-methylcoumarin (nhmec) was investigated. many more species than was previously recognized exhibited peptidase activities, albeit at lower levels than those already described for porphyromonas gingivalis. detectio ...19979460536
liver abscesses in feedlot cattle: a review.liver abscesses in slaughtered beef cattle result from aggressive grain-feeding programs. the incidence, averaging from 12 to 32% in most feedlots, is influenced by a number of dietary and management factors. liver abscesses represent a major economic liability to producers, packers, and ultimately consumers. besides liver condemnation, economic impacts include reduced feed intake, reduced weight gain, decreased feed efficiency, and decreased carcass yield. fusobacterium necrophorum, a member of ...19989464910
[septic thrombophlebitis of the internal jugular vein and lemierre syndrome].the authors report a case of lemierre's syndrome. this uncommon clinical entity is characterized by a septic internal jugular vein thrombosis with secondary metastatic abscesses and fusobacterium necrophorum septicemia, following an acute oropharyngeal infection. the diagnosis is primarily clinical and it should be suspected when a severe septicaemic illness, with pulmonary symptoms, occurs after an acute pharyngotonsillar infection. this article reviews the clinical picture, microbiology and tr ...19989553448
ammonia-hyperproducing bacteria from new zealand ruminants.pasture-grazed dairy cows, deer, and sheep were tested for the presence of ammonia-hyperproducing (hap) bacteria in roll tubes containing a medium in which tryptone and casamino acids were the sole nitrogen and energy sources. colonies able to grow on this medium represented 5.2, 1.3, and 11.6% of the total bacterial counts of dairy cows, deer, and sheep, respectively. a total of 14 morphologically distinct colonies were purified and studied further. restriction fragment length polymorphisms of ...19989572953
selecting therapy for serious infections in children: maximizing safety and efficacy.serious infections in children represent unique challenges for the treating physician. for the pediatric patient, considerations of drug toxicity are especially critical to avoid potential long-term complications of therapy. there are several advantages associated with using single, broad-spectrum, empiric antibiotic therapy, including reduced potential for drug-mediated toxicity or drug interactions and facilitation of home therapy. of the antibiotics available for monotherapy, the carbapenems ...19989635916
biochemical and biological characterizations and ribotyping of actinomyces pyogenes and actinomyces pyogenes-like organisms from liver abscesses in cattle.actinomyces pyogenes is the second most frequently encountered pathogen, next only to fusobacterium necrophorum, in liver abscesses of feedlot cattle. ninety-one isolates, presumptively identified as a. pyogenes, isolated from liver abscesses of cattle were studied. biochemical characteristics determined by the api 20 strep kit were similar to those reported previously for a. pyogenes isolated from other infections, except that 18% of isolates hydrolyzed esculin. nine isolates that resembled a. ...19989646478
postanginal septicaemia (lemmiere's disease) complicated by haemophagocytosis.we report a case of postanginal septicaemia complicated by bronchopneumonia and haemophagocytosis in a 19-year old male, presenting with severe thrombocytopenia. we believe that this is the first reported case of thrombocytopenia due to haemophagocytosis in this unusual condition.19989661952
case report: necrotic glossitis and sinusitis in a cow caused apparently by a fusobacterium necrophorum like unusual case of necrotic infection of the tongue and unilateral necrotic maxillar sinusitis, in a cow is reported. the prominent clinical findings were: a marked salivation, unilateral mucopurulent nasal discharge, diffuse necrotic lesions on the ventral side of the tongue and fetid breath. clinico-pathological and pathological findings and abnormalities in the haemogram were probably caused by the toxins produced by the causative organism--fusobacterium necrophorum like microorganism.19989674310
changes in bacterial populations in the colon of pigs fed different sources of dietary fibre, and the development of swine dysentery after experimental infection.swine dysentery (sd) is a disease which can be controlled by feeding a diet low in dietary fibre. the influence of source and inclusion level of dietary fibre both on bacterial populations in the colon, and on subsequent development of sd in pigs experimentally infected with serpulina hyodysenteriae was evaluated. in experiment 1, pigs were fed a low-fibre diet based on cooked rice and a animal protein supplement, or the same diet containing added insoluble (insp, fed as oaten chaff) or soluble ...19989750287
necrotising stomatitis associated with fusobacterium necrophorum in three sows. 19989787423
incidence and clinical epidemiology of necrobacillosis, including lemierre's syndrome, in denmark establish the incidence and describe the clinical epidemiology of necrobacillosis and lemierre's syndrome in denmark, the clinical records of all laboratory-recorded cases of septicaemia due to fusobacterium necrophorum biovar a, b, and c were reviewed retrospectively during a 6-year period. the incidence of necrobacillosis and lemierre's syndrome was 1.5 and 0.8 per million persons per year, respectively, showing a tendency to increase during the period. fusobacterium necrophorum was grown a ...19989796654
systemic and deep-seated infections caused by arcanobacterium haemolyticum.arcanobacterium haemolyticum has been implicated mainly in non-streptococcal pharyngitis and wound infections. rarely, it has been reported to cause systemic infection, often in combination with other pathogens. two cases of systemic and deep-seated infections caused by arcanobacterium haemolyticum are reported, and the literature is reviewed. sixteen cases of bacteremia and seven cases of non-bacteremic deep-seated have been published previously. eight of the bacteremic and two of the non-bacte ...19989796659
the erythrocyte receptor for fusobacterium necrophorum hemolysin: phosphatidylcholine as a possible attempt was made to determine the receptor for the hemolysin of fusobacterium necrophorum using horse erythrocyte or its membranes as target. the spectrum of erythrocyte sensitivity has indicated that horse, dog and mouse erythrocytes are highly sensitive whereas cattle, sheep, goat and chicken red blood cells are insensitive to this hemolysin. a high correlation between sensitivity and phosphatidylcholine content of the erythrocyte membranes was noted. binding of hemolysin to horse erythrocy ...19989812364
[investigations into the occurrence and the antibiotic susceptibility of gram negative anaerobes of the genera bacteroides, prevotella, porphyromonas and fusobacterium in specimens obtained from diseased animals].from different samples of 247 diseased animals (cattle, sheep, goat, horse, pig, dog, cat, rodent, zoo-animals), 410 strains of gram-negative anaerobes were cultured. 297 isolates (72.4%) could be differentiated to the species level by using cultural-biochemical methods, gaschromatography and cell-wall-lipidanalysis. they belonged to 29 different species. for an additional 113 strains (27.6%) only the genus could be determined. bacteria belonging to the genus fusobacterium occurred with the high ...19989818460
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