PMID(sorted descending)
killing of anaerobic pathogens by predatory bacteria.recently, the predation of bdellovibrio bacteriovorus on a periodontal pathogen has been described. the current study explores the potential antimicrobial activity of a range of predatory bacteria against key periodontal pathogens. a number of representatives from the bdellovibrio, bacteriovorax and peredibacter lineages (called 'balos') were tested for their activity towards a group of key periodontal pathogens and an optimal multiplicity of infection was established. as the oral cavity contain ...201021214872
predation of oral pathogens by bdellovibrio bacteriovorus 109j.periodontal diseases are multifactorial infections elicited by a complex of primarily gram-negative bacteria that interact with host tissues and lead to the destruction of the periodontal structures. bdellovibrio bacteriovorus is a gram-negative bacterium that preys upon other gram-negative bacteria. it was previously shown that b. bacteriovorus has an ability to attack and remove surface-attached bacteria or biofilms. in this study, we examined the host specificity of b. bacteriovorus strain 10 ...201021214870
effect of nicotine, cotinine and cigarette smoke extract on the neutrophil respiratory determine the effect of nicotine, cotinine and cigarette smoke extract (cse) on the neutrophil respiratory burst and their effect on activation of the nuclear factor-κb (nfκb) pathway in oral epithelium.201121214612
identification of periodontal pathogens and severity of periodontitis in patients with and without chronic kidney this study of patients with chronic periodontitis (cp), the severity of the disease and the main periodontal pathogens identified in patients with chronic kidney disease (ckd) were compared with those detected in individuals without systemic disease.201121211789
effect of smoking on subgingival microflora of patients with periodontitis in japan.smoking is a risk factor for periodontitis. to clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. the aim of this study was to gain an insight into the influence of smoking on the microflora of japanese patients with periodontitis.201121208407
[detection of putative periodontal pathogens of periodontitis with type 2 diabetes].to detect the putative periodontal pathogens in the subgingival plaque of chronic periodontitis (cp) patients with type 2 diabetes mellitus (dm) and to discuss the role of the periodontal pathogens in the cp and type 2 dm patients.201021163008
comparison of the use of the halimeter and the oral chroma™ in the assessment of the ability of common cultivable oral anaerobic bacteria to produce malodorous volatile sulfur compounds from cysteine and compare the use of the halimeter and the oral chroma™ to assess the ability of common oral anaerobic bacteria isolated from the kuwaiti population to produce volatile sulfur compounds (vscs).201021160219
microbiota of dentinal caries as assessed by reverse-capture checkerboard analysis.this study aimed to identify the microbiota of different layers of dentinal caries by using a culture-independent molecular biology approach.201021160186
antibacterial and anti-inflammatory activities of 4-hydroxycordoin: potential therapeutic benefits.4-hydroxycordoin (1), a natural isopentenyloxychalcone, is a plant secondary metabolite that is relatively rare. since there are very few reports about the biological activities of 1, its potential benefits for periodontal disease were investigated. a marked and dose-dependent antibacterial activity of 1 was observed against the three major periodontal pathogens, porphyromonas gingivalis, fusobacterium nucleatum, and prevotella intermedia. moreover, compound 1 showed an antiadhesion effect, sinc ...201021158427
denaturing gradient gel electrophoresis analysis with different primers of subgingival bacterial communities under mechanical debridement.dgge of 16s rdna is one of the most frequently used methods to study microbial communities. in this study, the dgge profiles of different 16s rdna regions of the periodontal pathogens porphyromonas gingivalis, fusobacterium nucleatum, and prevotella nigrescens were investigated. the results suggested that v3-v5 and v6-v8 fragments may be suitable for community analysis of subgingival bacteria. further analysis of subgingival samples with v3-v5 and v6-v8 regions as target fragments suggested that ...201021155359
systemic inflammatory responses in progressing periodontitis during pregnancy in a baboon model.this study tested the hypothesis that pregnant female baboons exhibit increased levels of various inflammatory mediators in serum resulting from ligature-induced periodontitis, and that these profiles would relate to periodontal disease severity/extent in the animals. the animals were sampled at baseline (b), mid-pregnancy (mp; two quadrants ligated) and at delivery (d; four quadrants ligated). all baboons developed increased plaque, gingival inflammation and bleeding, pocket depths and attachme ...201021070210
intracellular degradation of fusobacterium nucleatum in human gingival epithelial cells.the role of fusobacterium nucleatum in oral health and disease is controversial. we have previously shown that f. nucleatum invades gingival epithelial cells. however, the destiny of the internalized f. nucleatum is not clear. in the present study, the intracellular destiny of f. nucleatum and its cytopathic effect on gingival epithelial cells were studied. the ability of f. nucleatum and seven other oral bacterial species to invade immortalized human gingival epithelial (hok-16b) cells were com ...201021057979
early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man.aim: to compare the early bacterial colonization and soft tissue health of mucosa adjacent to zirconia (zro(2) ) and titanium (ti) abutment surfaces in vivo. materials and methods: twenty edentulous subjects received two endosseous mandibular implants. the implants were fitted with either a zro(2) or a ti abutment (non-submerged implant placement, within-subject comparison, left-right randomization). sulcular bacterial sampling and the assessment of probing pocket depth, recession and bleeding o ...201021054554
photoinactivation of f. nucleatum and p. gingivalis using the ruthenium-based rd3 sensitizer and a conventional halogen lamp.the photodynamic therapy (pdt) is an alternative method to suppress oral pathogens by the activation of a photosensitizer with laser light. the aim of this study was to investigate the phototoxic effect of three ruthenium-based photosensitizers on fusobacterium nucleatum and porphyromonas gingivalis.201021036348
setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.caries and periodontitis are important human diseases associated with formation of multi-species biofilms. the involved bacteria are intensively studied to understand the molecular basis of the interactions in such biofilms. this study established a basic in vitro single and mixed-species culture model for oral bacteria combining three complimentary methods. the setup allows a rapid screening for effects in the mutual species interaction. furthermore, it is easy to handle, inexpensive, and repro ...201020957048
murine fetoplacental infection models. 201020956576
oxygen tension modulates the cytokine response of oral epithelium to periodontal bacteria.there is an inverse relationship between pocket depth and pocket oxygen tension with deep pockets being associated with anaerobic bacteria. however, little is known about how the host tissues respond to bacteria under differing oxygen tensions within the periodontal pocket.201020955352
bactericidal activity of stabilized chlorine dioxide as an endodontic irrigant in a polymicrobial biofilm tooth model system.the purpose of this study was to determine bactericidal efficacy of 0.04% stabilized chlorine dioxide, 3% sodium hypochlorite, 2% chlorhexidine gluconate, and sterile distilled water in a polymicrobial biofilm model.201020951304
effect of biofilm on the repair bond strengths of composites.composite restorations degrade during wear, but it is unknown how wear affects the composite surface and influences composite-to-composite bonding in minimally invasive repair. here, it is hypothesized that in vitro exposure of composites to oral biofilm yields clinically relevant degradation of composite surfaces, and its influence on composite-to-composite bonding is determined. biofilms on composite surfaces in vitro increased their roughness and decreased filler particle exposure, except for ...201020940354
[effect of compound chinese traditional medicine on infected root canal bacteria biofilm].to assess the efficacy of compound chinese traditional medicine(ctm), which composed of gallic acid, magnolol and polysaccharide of blettila striata, against the infected root canal bacterial biofilm.201020871960
isolation of a novel bacteriophage specific for the periodontal pathogen fusobacterium nucleatum.fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. we isolated a new bacteriophage (fnpφ02) that specifically infects this bacterium. transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. the size of the phage genome was estimated to be approximately 59 kbp of double-stranded ...201020851973
apical root canal microbiota as determined by reverse-capture checkerboard analysis of cryogenically ground root samples from teeth with apical periodontitis.bacteria located in the apical root canal system potentially participate in the pathogenesis of apical periodontitis. detection and identification of apical bacteria can be compromised because of limitations in conventional sampling and identification procedures. this study identified several bacterial taxa in the apical and middle/coronal segments of primarily infected root canal system by using pulverized root segments and a culture-independent molecular method.201020850664
antimicrobial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria.the aim of this study is to assess the antibacterial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria.201020849395
fusobacterium nucleatum-associated beta-defensin inducer (fad-i): identification, isolation, and functional evaluation.human β-defensins (hbds) are small, cationic antimicrobial peptides, secreted by mucosal epithelial cells that regulate adaptive immune functions. we previously reported that fusobacterium nucleatum, a ubiquitous gram-negative bacterium of the human oral cavity, induces human β-defensin 2 (hbd2) upon contact with primary oral epithelial cells. we now report the isolation and characterization of an f. nucleatum (atcc 25586)-associated defensin inducer (fad-i). biochemical approaches revealed a ce ...201020847052
fusobacterium nucleatum binding to complement regulatory protein cd46 modulates the expression and secretion of cytokines and matrix metalloproteinases by oral epithelial cells.periodontitis is a chronic inflammatory disease that results in the destruction of the supporting tissues of the teeth. gingival epithelial cells are an important mechanical barrier and participate in the host inflammatory response to periodontopathogens. the aim of the present study is to investigate the capacity of fusobacterium nucleatum to bind to the complement regulatory protein cd46 expressed by oral epithelial cells and to determine the impact of the binding on the gene expression and pr ...201120843232
a 6-month study of the effects of 0.3% triclosan/copolymer dentifrice on dental implants.supportive therapy to maintain dental implants is increasingly important. this study examined the effect of a 0.3% triclosan/2% copolymer dentifrice on oral biofilms and gingival inflammation (gi) on dental implants and peri-implant tissues.201120831669
fusobacterium nucleatum outer membrane proteins fap2 and radd induce cell death in human lymphocytes.bacterially induced cell death in human lymphocytes is an important virulence factor for pathogenic bacteria. previously discovered mechanisms of bacterially induced cell death are predominantly based on the transfer of bacterial proteins to the target host cell, such as the toxins secreted through type i, ii, and vi secretion systems or effector proteins injected through type iii, iv, and vb secretion systems. here, we report a mechanism employed by the gram-negative oral pathogen fusobacterium ...201020823215
polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (dcs), at the mucosal surface.201120798974
a brain abscess following dental extractions in a patient with hereditary hemorrhagic telangiectasia.oral and maxillofacial surgeons must be aware of the potentially life-threatening complications of dental extractions in patients with hereditary haemorrhagic telangiectasia because of their high prevalence of pulmonary arteriovenous fistulas. despite the lack of evidence-based guidelines, antibiotic cover should be given to patients with hht who require oral surgery according to the same rules as those used for patients at high risk of bacterial endocarditis.201020719417
vaccination of mice with porphyromonas gingivalis or fusobacterium nucleatum modulates the inflammatory response, but fails to prevent experimental assess the potential of using vaccination with porphyromonas gingivalis or fusobacterium nucleatum, in modulating local subcutaneous inflammatory response and alveolar bone loss following coinfection with both bacteria.201020670341
energy dose parameters affect antimicrobial photodynamic therapy-mediated eradication of periopathogenic biofilm and planktonic cultures.this study evaluated the in vitro efficacy of a commercially available apdt system in eradication of the periopathogens porphyromonas gingivalis, fusobacterium nucleatum, and aggregatibacter actinomycetemcomitans in both planktonic and biofilm cultures.201020666581
fusobacterium nucleatum regulation of neutrophil transcription.abnormal neutrophil responses have been observed in periodontitis patients, including hyper-reactivity in terms of production of reactive oxygen species (ros) following exposure to the key quorum-sensing plaque bacterium, fusobacterium nucleatum. this study was designed to characterize the transcriptional response of neutrophils to f. nucleatum.201120663022
microbiota around teeth and dental implants in periodontally healthy, partially edentulous patients: is pre-implant microbiological testing relevant?this study aimed to assess the prevalence of seven periodontal marker pathogens, before implant placement and 1 yr after loading, in periodontally healthy individuals and to assess the long-term effectiveness of pre-implant reduction of pathogens to below threshold levels. in 93 individuals needing single tooth replacement, pooled subgingival microbiological samples from standard sites were cultured and analyzed before implant treatment and 1 yr after loading. threshold levels commonly used in p ...201020662908
examination of periodontal pathogens in stenotic valve specimens and in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis.periodontitis may be a risk factor for atherosclerosis and coronary heart disease. the influence of periodontal pathogens in cardiovascular diseases needs further investigation. therefore, the aims of this clinical study are: to test the presence of periodontal bacteria dna in aortic valves and to assess the concomitant presence of the same periodontal bacteria dna in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. nineteen consecutive patients (12 ma ...201020646351
antiaggregation potential of berry fractions against pairs of streptococcus mutans with fusobacterium nucleatum or actinomyces naeslundii.coaggregation is an interspecies adhesion process, which is essential to the development of dental plaque. this is an in vitro study of the composition of the soluble solids in the berry juice molecular size fractions (<10 kda, fi; 10-100 kda, fii; >100 kda, fiii) derived from apple, bilberry, blackcurrant, cloudberry, crowberry and lingonberry and their ability to inhibit and reverse coaggregation of the pairs of common species in dental plaque: streptococcus mutans with fusobacterium nucleatum ...201120623601
new aspects and new concepts of maintaining "microbiological" health.the human mouth is host to a diverse collection of microorganisms including bacteria, viruses, fungi and protozoa. recent advances using molecular methods for the analysis of complex bacterial communities have demonstrated the richness of the oral bacterial biota and the presence of numerous previously undescribed lineages. dental plaque forms in a structured way with pioneer species able to colonise pellicle-coated enamel followed by secondary plaque formers such as fusobacterium nucleatum that ...201020621241
differential and coordinated expression of defensins and cytokines by gingival epithelial cells and dendritic cells in response to oral bacteria.epithelial cells and dendritic cells (dcs) both initiate and contribute to innate immune responses to bacteria. however, much less is known about the coordinated regulation of innate immune responses between gecs and immune cells, particularly dcs in the oral cavity. the present study was conducted to investigate whether their responses are coordinated and are bacteria-specific in the oral cavity.201020618959
hiv-1 reactivation induced by the periodontal pathogens fusobacterium nucleatum and porphyromonas gingivalis involves toll-like receptor 2 [corrected] and 9 activation in monocytes/macrophages.although oral coinfections (e.g., periodontal disease) are highly prevalent in human immunodeficiency virus type 1-positive (hiv-1(+)) patients and appear to positively correlate with viral load levels, the potential for oral bacteria to induce hiv-1 reactivation in latently infected cells has received little attention. we showed that hiv-1 long terminal repeat (ltr) promoter activation can be induced by periodontopathogens in monocytes/macrophages; nevertheless, the mechanisms involved in this ...201020610663
[usefulness and limit of gram staining smear examination].gram staining is one of the most simple and inexpensive methods for the rapid diagnosis of bacterial and fungal infections. it yields results much faster than culture, and provides important data for the patient's treatment and prognosis. however, a difference exists in the quality and quantity of information yielded by gram staining smears based on the experience and knowledge of those conducting the tests. therefore, a risk of misdiagnosis based on the information obtained from gram staining s ...201020560458
antibiotic resistance of subgingival species in chronic periodontitis patients.the increasing rate of resistance of microorganisms to penicillin and other antibiotics has generated concern among health authorities in latin america. the present investigation determined the in vitro susceptibility of porphyromonas gingivalis, fusobacterium nucleatum, black-pigmented prevotella spp. and aggregatibacter actinomycetemcomitans to metronidazole, amoxicillin, amoxicillin/clavulanic acid, clindamycin and moxifloxacin in patients with chronic periodontitis.201020546113
antimicrobial activity of n-6, n-7 and n-9 fatty acids and their esters for oral microorganisms.this study is to assess the antibacterial activity of omega-6, -7, -9 (n-6, n-7, n-9) fatty acids against various oral microorganisms.201020541177
folding of a transcriptionally acting preq1 riboswitch.7-aminomethyl-7-deazaguanine (preq(1)) sensitive mrna domains belong to the smallest riboswitches known to date. although recent efforts have revealed the three-dimensional architecture of the ligand-aptamer complex less is known about the molecular details of the ligand-induced response mechanism that modulates gene expression. we present an in vitro investigation on the ligand-induced folding process of the preq(1) responsive rna element from fusobacterium nucleatum using biophysical methods, ...201020534493
gram-negative periodontal bacteria induce the activation of toll-like receptors 2 and 4, and cytokine production in human periodontal ligament cells.periodontitis is a bacterially induced chronic inflammatory disease. toll-like receptors (tlrs), which could recognize microbial pathogens, are important components in the innate and adaptive immune systems. both qualitatively and quantitatively distinct immune responses might result from different bacteria stimulation and the triggering of different tlrs. this study explores the interaction of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, and aggregatibacter actinomy ...201020528699
antimicrobial efficacy of 3 oral antiseptics containing octenidine, polyhexamethylene biguanide, or citroxx: can chlorhexidine be replaced?use of oral antiseptics decreases the bacterial load in the oral cavity.201020518635
effects of a composition containing lactoferrin and lactoperoxidase on oral malodor and salivary bacteria: a randomized, double-blind, crossover, placebo-controlled clinical trial.we report a clinical trial of the effects of test tablets containing bovine lactoferrin and lactoperoxidase on oral malodor and salivary bacteria. fifteen subjects with volatile sulfur compounds (vscs) in mouth air above the olfactory threshold (h(2)s >1.5 or ch(3)sh >0.5 ng/10 ml) as detected by gas chromatography were enrolled in the trial. either a test or a placebo tablet was ingested twice at 1-h intervals in two crossover phases. mouth air was monitored for vsc levels at the baseline befor ...201020512389
[reversible metronidazole-induced encephalopathy and hypertrophic olivary degeneration]. 201020508563
mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. ii. microbiological results.peri-implantitis is common in patients with dental implants. we performed a single-blinded longitudinal randomized study to assess the effects of mechanical debridement on the peri-implant microbiota in peri-implantitis lesions.201020507380
the recolonization hypothesis in a full-mouth or multiple-session treatment protocol: a blinded, randomized clinical test recolonization of periodontal lesions after full-mouth scaling and root planing (fm-srp) or multiple session-srp (ms-srp) in a randomized clinical trial and whether fm-srp and ms-srp result in different clinical outcomes.201020507375
in vitro cytokine responses to periodontal pathogens: generalized aggressive periodontitis is associated with increased il-6 response to porphyromonas gingivalis.generalized aggressive periodontitis (gagp) is an inflammatory condition resulting in destruction of tooth-supporting tissues. we examined the production of il-1beta, il-6, tumour necrosis factor (tnf)-alpha, il-12 and il-10 in cultures of peripheral mononuclear cells (mnc) from 10 patients with gagp and 10 controls stimulated with periodontal pathogens or a control antigen, tetanus toxoid (tt) in the presence of autologous serum. the pathogens used were porphyromonas gingivalis, prevotella inte ...201020500696
identification of immunological parameters associated with the alveolar bone level in periodontal patients.the present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum igg titer against periodontopathogenic bacteria, the expression of the helper t-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (hsp) epitope peptide from p. gingivalis hsp60.201020498762
competition between yogurt probiotics and periodontal pathogens in investigate the competition between probiotics in bio-yogurt and periodontal pathogens in vitro.201020491536
production of indole from l-tryptophan and effects of these compounds on biofilm formation by fusobacterium nucleatum atcc 25586.the l-tryptophan degradation product indole is a purported extracellular signaling molecule that influences biofilm formation in various bacteria. here we analyzed the mechanisms of indole production in fusobacterium nucleatum and the effects of tryptophan and indole on f. nucleatum planktonic and biofilm cells. the amino acid sequence deduced from the fn1943 gene in f. nucleatum atcc 25586 was 28% identical to that deduced from tnaa in escherichia coli, which encodes tryptophanase catalyzing th ...201020472741
antibacterial effects of mdpb against anaerobes associated with endodontic investigate the antibacterial effects of 12-methacryloyloxydodecylpyridinium bromide (mdpb), an antibacterial monomer synthesized by combining quaternary ammonium with a methacryloyl group, against three anaerobes associated with endodontic infections using planktonic and biofilm cells.201020456514
communication: antimicrobial activity of smap28 with a targeting domain for porphyromonas gingivalis.antibiotic therapy is often used with mechanical therapy to treat periodontal disease. however, complications associated with antibiotic use can occur. a 'bacteria-specific' targeted approach would eliminate some of these complications and kill specific periodontopathogens without harming the commensal bacteria. one such approach is to couple antimicrobial peptides to a ligand, pheromone, or antibody specific for the periodontopathogen, porphyromonas gingivalis. to assess the feasibility of this ...201020454638
innate inflammatory responses of human decidual cells to periodontopathic bacteria.the purpose of this study was to test the hypothesis that periodontopathic bacteria exert potent proinflammatory effects in human decidua.201020452492
production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in fusobacterium nucleatum subsp. nucleatum atcc 25586.fusobacterium nucleatum produces a large amount of the toxic metabolite hydrogen sulfide in the oral cavity. here, we report the molecular basis of f. nucleatum h(2)s production, which is associated with two different enzymes: the previously reported cdl (fn1220) and the newly identified lcd (fn0625). sds-page analysis with activity staining revealed that crude enzyme extracts from f. nucleatum atcc 25586 contained three major h(2)s-producing proteins. two of the proteins with low molecular mass ...201020413556
[effect of periodontal mechanical treatment on periodontal pathogenic bacteria in gingival crevicular fluid of chronic periodontitis patients].to evaluate the subgingival prevalent rates of 6 periodontal pathogenic bacteria in gingival crevicular fluids of cp patients before and after treatment, to analyze the relationship between the prevalent variance and periodontal clinical parameters, and to provide a microbiologic method of evaluating curative effect and estimating the prognosis.201020396365
detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16s rrna gene pcr.we describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16s rrna gene pcr. simultaneous detection of fusobacterium nucleatum and porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16s rrna genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.201020392909
antimicrobial activity of super-oxidised water against oral microorganisms.the radical anion of oxygen (o(-)) is extremely oxidative and shows high reactivity. in this study, the antibacterial activity of water super-oxidised water containing high concentration of o(-) (o(-)-water) was tested against cultured planktonic cells of cariogenic bacteria, periodontopathic bacteria and candida albicans.201020381790
mixture of periodontopathogenic bacteria influences interaction with kb cells.the purpose of this study was to investigate the adhesion and invasion of periodontopathogenic bacteria in varied mixed infections and the release of interleukins from an epithelial cell line (kb cells).201020380884
case records of the massachusetts general hospital. case 10-2010. a 37-year-old woman with weakness and a mass in the brain. 201020375410
biofilm formation enhances the oxygen tolerance and invasiveness of fusobacterium nucleatum in an oral mucosa culture model.the present study evaluates the survival capability of fusobacterium nucleatum strains in an aerobic environment and compares the invasive capability of f. nucleatum in biofilm and planktonic forms in an organotypic cell culture (occ) model.201020350156
full-mouth antimicrobial photodynamic therapy in fusobacterium nucleatum-infected periodontitis patients.the purpose of this study is to clinically and microbiologically evaluate the effect of photodynamic therapy (pdt) as a full-mouth procedure in fusobacterium nucleatum-infected patients with periodontitis.201020350153
supernatants from oral epithelial cells and gingival fibroblasts modulate human immunodeficiency virus type 1 promoter activation induced by periodontopathogens in monocytes/macrophages.bacterial and host cell products during coinfections of human immunodeficiency virus type 1-positive (hiv-1(+)) patients regulate hiv-1 recrudescence in latently infected cells (e.g. t cells, monocytes/macrophages), impacting highly active antiretroviral therapy (haart) failure and progression of acquired immunodeficiency syndrome. a high frequency of oral opportunistic infections (e.g. periodontitis) in hiv-1(+) patients has been demonstrated; however, their potential to impact hiv-1 exacerbati ...201020331801
a novel bioactivity of omega-3 polyunsaturated fatty acids and their ester oil, enriched in omega-3 polyunsaturated fatty acids (n-3 pufa), is widely used as a dietary or nutritional supplement with numerous benefits, including as an anti-inflammatory particularly linked to atherosclerosis. while n-3 pufa have been suggested to be able to improve oral health through a reduction in inflammation through elevations in these fatty acids in serum and cellular membranes, information is lacking for the possibility that these fatty acids could directly impact the survival ...201020331795
endodontic photoactivated disinfection using a conventional light source: an in vitro and ex vivo study.the antimicrobial effect of photoactivated disinfection (pad) using toluidine blue and an led lamp was tested on endodontic pathogens in planktonic suspension and after inoculation into extracted teeth. irradiation time was limited to 30 seconds.201020303060
effect of alkaline growth ph on the expression of cell envelope proteins in fusobacterium nucleatum.fusobacterium nucleatum is a gram-negative anaerobic organism that plays a central role in the development of periodontal diseases. the progression of periodontitis is associated with a rise in ph of the gingival sulcus which promotes the growth and expression of virulence factors by periodontopathic bacteria. we have previously reported that the expression of specific cytoplasmic proteins is altered by a shift in growth ph. in the present study we have compared cell envelope protein expression ...201020299401
photodynamic therapy in periodontal therapy: microbiological observations from a private recent years, the combination of laser light and photosensitizer known as photodynamic therapy (pdt) has been used in periodontal therapy. however, there are not enough clinical studies to fully evaluate the effects of pdt on the periodontal tissues. this microbiological study examined the effects of pdt on the periodontal bacteria in combination with scaling and root planing (srp) in the same group of patients by randomly selecting pdt or srp for use in different quadrants of the mouth. for ...201020236906
antimicrobial effect of rosa damascena extract on selected endodontic pathogens.the purpose of this study was to compare the antimicrobial activity of a plant-derived extract (2 percent rosa damascena extract) with 5.25 percent sodium hypochlorite (naocl) and 2 percent chlorhexidine (chx) on selected endodontic pathogens. the minimum inhibitory concentrations (mics) of 2 percent rose extract and 2 percent chx for test microorganisms, except f. nucleatum, were lower than that of 5.25 percent naocl. all solutions were able to kill all test microorganisms after one minute.201020232691
potentiation of bacterial killing activity of zinc chloride by pyrrolidine dithiocarbamate.zinc has antimicrobial activity and zinc salts including zinc chloride (zncl(2)) have been used for the control of oral malodor. in this study, we hypothesized that pyrrolidine dithiocarbamate (pdtc), a zinc ionophore, may enhance antimicrobial efficacy of zncl(2). the bactericidal effectiveness of zncl(2) alone (0.5-8 mm) or in combination with pdtc (1 or 10 microm) was evaluated by in vitro short (1 h) time-killing assays against fusobacterium nucleatum and porphyromonas gingivalis. only a sli ...201020221728
detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. micro-ident and micro-ident plus (hain lifescience gmbh, nehren, germany) tests are two commercially available rapid pcr-based methods for the identification and quantification of putative periodontopathogen bacteria. in this study, we compared these commercial pcr-based hybridization methods with conventional anaerobic culture technique. a total of 36 subgingival plaque sa ...201020219690
interplay of protease-activated receptors and nod pattern recognition receptors in epithelial innate immune responses to bacteria.protease-activated receptors (pars), nucleotide-binding oligomerization domain (nod) receptors and toll-like receptors (tlrs) play a role in innate immunity, but little is known about interaction between these receptors. the goal of this study was to investigate how silencing one receptor affects the expression of other receptors and downstream innate immune markers in response to bacteria. human gingival epithelial cells (gecs) were transfected with sirna specific for par1 or par2, then stimula ...201020219537
[anaerobic gram-negative bacilli involved in the etiopathogeny of the abscesses of superficial fascial spaces of the face and neck].the aim of this study was to isolate and identify at species level the strains of anaerobic gram-negative bacilli (gnb) from pus samples collected in patients with abscesses of fascial spaces of the face and neck. microscopy of gram-stained smears and cultures were performed in each specimen. the strictly anaerobic gnb strains were identified using the conventional methods of diagnosis and the rapid id 32 a system. in addition, the other strains isolated in association with these bacteria were i ...200820201270
oral biofilm architecture on natural teeth.periodontitis and caries are infectious diseases of the oral cavity in which oral biofilms play a causative role. moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and biofilm resistance to antibiotics, due to their widespread presence and accessibility. despite descriptions of initial plaque formation on the tooth surface, studies on mature plaque and plaque structure below the gum are limited to landmark studies from the 1970s, without app ...201020195365
quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction.chronic periodontitis is a highly prevalent endogenous polymicrobial disease. to better understand the etiology of the disease a quantitative approach is mandatory and real-time pcr is the molecular technique currently preferred to achieve this purpose. taking into account that such a kind of study is still scarce, we aimed to evaluate the association between periodontal microbiota and chronic periodontitis. a total of 60 low-income age-matched female adults, 30 with chronic periodontitis and 30 ...201020193770
vaccination targeting surface foma of fusobacterium nucleatum against bacterial co-aggregation: implication for treatment of periodontal infection and halitosis.the mechanical therapy with multiple doses of antibiotics is one of modalities for treatment of periodontal diseases. however, treatments using multiple doses of antibiotics carry risks of generating resistant strains and misbalancing the resident body flora. we present an approach via immunization targeting an outer membrane protein foma of fusobacterium nucleatum (f. nucleatum), a central bridging organism in the architecture of oral biofilms. neutralization of foma considerably abrogated the ...201020189489
lemierre syndrome: a japanese patient returning from thailand.after returning from thailand, a 23-year-old japanese man was admitted because of fever, sore throat, neck pain, and chest pain. contrasted-enhanced ct scanning of his neck revealed an absence of flow through the right internal jugular vein representing thrombosis, and moreover, an increase in wall thickness of the right internal jugular vein and enhancement of the surrounding tissue representing thrombophlebitis. lung abscesses were also identified by a chest ct scan. fusobacterium nucleatum wa ...201020177952
the relationship between physiologic halitosis and periodontopathic bacteria of the tongue and gingival determine the influence of oral status on halitosis, the relationship between halitosis and periodontopathic bacteria present in plaque on the tongue and the subgingival sulcus was examined in 62 periodontally healthy adults. halitosis indicators used were the organoleptic score; gas chromatography results [total volatile sulfur compounds (vscs) = h(2)s + ch(3)sh + (ch(3))(2)s]; halimeter values; and the results of three clinical tests, plaque control record (plcr), plaque index (pli), and to ...201020155507
central role of the early colonizer veillonella sp. in establishing multispecies biofilm communities with initial, middle, and late colonizers of enamel.human dental biofilm communities comprise several species, which can interact cooperatively or competitively. bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of one-, two- and three-species communities growing on saliva as the sole nutritional source. we investigated single-species and multispecies colonization by using known initial, early ...201020154130
effects of a mouthwash with chlorine dioxide on oral malodor and salivary bacteria: a randomized placebo-controlled 7-day trial.previous research has shown the oxidizing properties and microbiological efficacies of chlorine dioxide (clo2). its clinical efficacies on oral malodor have been evaluated and reported only in short duration trials, moreover, no clinical studies have investigated its microbiological efficacies on periodontal and malodorous bacteria. thus, the aim of this study was to assess the inhibitory effects of a mouthwash containing clo2 used for 7 days on morning oral malodor and on salivary periodontal a ...201020152022
the antimicrobial efficacy of commercial dentifrices.this investigation compared the effects of a fluoride dentifrice and toothpastes formulated with antimicrobial ingredients (stannous fluoride and triclosan/copolymer) on oral micro-organisms, including those found in samples taken from the human oral cavity. microbiological techniques determined the minimum inhibitory concentrations (mics) of each dentifrice necessary to inhibit the growth of bacterial strains from the healthy oral cavity, as well as those found in dental caries, periodontal dis ...201020129893
effects of methyl gallate and gallic acid on the production of inflammatory mediators interleukin-6 and interleukin-8 by oral epithelial cells stimulated with fusobacterium nucleatum.interactions between periodontal bacteria and human oral epithelial cells can lead to the activation and expression of a variety of inflammatory mediators in epithelial cells. fusobacterium nucleatum is a filamentous human pathogen that is strongly associated with periodontal diseases. this study examined the effects of methyl gallate (mg) and gallic acid (ga) on the production of inflammatory mediators, interleukin (il)-6 and il-8, by oral epithelial cells stimulated by f. nucleatum. in a real- ...201020127471
the bactericidal effect of a genius nd:yag evaluate the 'in vitro' bactericidal effect of the nd:yag laser (genius, mølsgaard dental, copenhagen, denmark) on six periodontal pathogens.201020096084
epithelial cell pro-inflammatory cytokine response differs across dental plaque bacterial species.the dental plaque is comprised of numerous bacterial species, which may or may not be pathogenic. human gingival epithelial cells (hgecs) respond to perturbation by various bacteria of the dental plaque by production of different levels of inflammatory cytokines, which is a putative reflection of their virulence. the aim of the current study was to determine responses in terms of interleukin (il)-1beta, il-6, il-8 and il-10 secretion induced by porphyromonas gingivalis, aggregatibacter actinomyc ...201020096064
term stillbirth caused by oral fusobacterium nucleatum.intrauterine infection is a recognized cause of adverse pregnancy outcome, but the source of infection is often undetermined. we report a case of stillbirth caused by fusobacterium nucleatum that originated in the mother's mouth.201020093874
use of a novel assay to evaluate enzymes that produce hydrogen sulfide in fusobacterium evaluate enzymes that produce hydrogen sulfide (h(2)s) in species of fusobacterium nucleatum, we developed an assay based on sds-polyacrylamide gel electrophoresis with renaturation followed by active staining. this assay provided precise insight into the enzymes that produce h(2)s in terms of their number and molecular weights.201020093145
efficacy of a low-concentration chlorhexidine mouth rinse in non-compliant periodontitis patients attending a supportive periodontal care programme: a randomized clinical assess the clinical and microbiological efficacy of a 0.05% chlorhexidine and 0.05% cetyl-pyridinium chloride mouth rinse in supportive periodontal care (spc) in patients with inadequate plaque control.201020088984
substrate-dependent proton antiport in neurotransmitter:sodium symporters.neurotransmitter-sodium symporters (nss), targets for psychostimulants and therapeutic drugs, have a critical role in neurotransmission. whereas eukaryotic nss show chloride-dependent transport, bacterial nss feature cl(-)-independent substrate transport. recently we showed that mutation of an acidic residue near one of the sodium ion-binding sites in leut of aquifex aeolicus or tyt1 of fusobacterium nucleatum renders substrate binding and/or transport cl(-) dependent. we reasoned that the negat ...201020081826
[pelvic actinomycosis: a misleading picture].pelvic actinomycosis is a rare chronic suppurative granulomatous disease associated with an intrauterine contraceptive device. next pelvic organs can be affected but also more distant organs, to lead to different clinical situations. the diagnosis is therefore often difficult. we report here the case of a woman who presents a particular extensive pelvic actinomycosis diagnosed after surgical treatment with important morbidity. this case shows the interest of evoking the diagnosis in an evocative ...201020074892
in vitro killing activity of crevicular concentrations of tinidazole plus common oral antibiotics against high-density mixed inocula of periodontal pathogens in strict anaerobic conditions.odontogenic infections are polymicrobial. this study explores the in vitro killing activity by concentrations similar to those found in crevicular fluid of tinidazole in combination with amoxicillin/clavulanic acid, clindamycin and levofloxacin against four groups of high-density mixed inocula of anaerobes (prevotella buccae, fusobacterium nucleatum, and veillonella spp.) and facultative (capnocytophaga spp. and streptococcus spp.) isolates of periodontal pathogens.201020059425
microbiologic testing and outcomes of full-mouth scaling and root planing with or without amoxicillin/metronidazole in chronic has been suggested that use of systemic antibiotics should be limited to patients with specific microbiologic profiles. the main purpose of the present analysis was to study whether microbiologic testing before therapy was of value in predicting which patients would specifically benefit from adjunctive amoxicillin and metronidazole, given in the context of full-mouth scaling and root planing (srp) within 48 hours.201020059413
nod1 and nod2 mediate sensing of periodontal bacterial infection, nucleotide-binding oligomerization domain (nod) 1 and nod2 induce innate immune responses by recognizing fragments of the bacterial component peptidoglycan (pgn). to determine the roles of these receptors in detection of periodontal pathogens, we stimulated human embryonic kidney cells expressing nod1 or nod2 with heat-killed porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, and fusobacterium nucleatum or their soluble pgns (spgns). all bacteria and their s ...201020040739
bacterial adhesins to host components in periodontitis. 201020017793
fusobacterium nucleatum envelope protein foma is immunogenic and binds to the salivary statherin-derived peptide.we have previously shown that one of the minimal active regions of statherin, a human salivary protein, for binding to fusobacterium nucleatum is a yqpvpe amino acid sequence. in this study, we identified the foma protein of f. nucleatum, which is responsible for binding to the statherin-derived yqpvpe peptide. overlay analysis showed that a 40-kda protein of the f. nucleatum cell envelope (40-kda ce) specifically bound to the yqpvpe peptide. the equilibrium association constant between the affi ...201020008529
identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach.this study identified the bacterial taxa enduring endodontic treatment procedures by using a combined 16s ribosomal rna-based reverse-transcriptase polymerase chain reaction (rt-pcr) and reverse-capture checkerboard hybridization approach.201020003934
transmission of periodontopathic bacteria from natural teeth to implants.purpose: prevention of peri-implantitis is essential for the success of implant rehabilitation. infection by periodontopathic bacteria is a major cause of peri-implantitis. the aim of the present study was to identify the source of peri-implant colonization by periodontopathic bacteria. materials and methods: twenty-one patients with implants were enrolled in the study. subgingival plaque samples from the adjacent, occluding, and contralateral natural teeth were collected prior to second-stage s ...200920002682
application of rpob and zinc protease gene for use in molecular discrimination of fusobacterium nucleatum subspecies.fusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, f. nucleatum subsp. fusiforme, f. nucleatum subsp. vincentii, and f. nucleatum subsp. animalis) based on several phenotypic characteristics and dna-dna hybridization patterns. however, the methods for detecting or discriminating the clinical isolates of f. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. the ...201019955278
fusobacterium bacteremia: clinical significance and outcomes.fusobacterium bacteremia is uncommon, accounting for approximately 0.9% of patients with bacteremia. the objectives of this study were to evaluate the incidence and clinical significance of blood cultures positive for fusobacterium spp., risk factors for mortality, and the impact of antimicrobial therapy on clinical outcomes.200919949758
methamphetamine cytotoxicity and effect on lps-stimulated il-1beta production by human monocytes.methamphetamine (meth) abuse is associated with "meth mouth", characterized by rampant dental decay and destruction of periodontal bone and soft tissues. in periodontitis, monocyte/macrophages, stimulated by bacterial lipopolysaccharide (lps), produce interleukin-1beta (il-1beta), contributing to bone and soft tissue degradation. effects of meth on monocyte/macrophages and its role in periodontitis are unknown. the objective of this study was to determine meth cytotoxicity and effects on constit ...201019945523
acute appendicitis is characterised by local invasion with fusobacterium nucleatum/necrophorum.acute appendicitis is a local intestinal inflammation with unclear origin. the aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls.201119926616
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