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specificity of monoclonal antibodies reactive with fusobacterium nucleatum: effect of formalin fixation.monoclonal antibody specific for fusobacterium nucleatum was reacted with untreated and formalin fixed f. nucleatum cells by an enzyme-linked immunosorbent assay (elisa) and by indirect immunofluorescence. treatment of bacterial cells with formalin destroyed the antigenic determinant responsible for reactivity with this monoclonal antibody in both assays. formalin fixation had no effect on hemagglutination activity (ha) of f. nucleatum cells or reactivity with polyvalent rabbit antiserum in doub ...19852415637
target size of ca-pumps in pig coronary artery smooth muscle.the target sizes of the oxalate-independent ca uptake by the plasma membrane enriched fraction f2, and the oxalate-stimulated ca uptake by a fraction f3 slightly enriched in the endoplasmic reticulum were determined by radiation inactivation. the oxalate-independent ca uptake was inactivated with a d37 value of 1.96 +/- 0.30 mrad but the oxalate-stimulated ca uptake had a d37 value of 0.45 +/- 0.07 mrad. thus, in the smooth muscle the oxalate-stimulated ca uptake appeared to be due to a structur ...19852415797
[malaria prevention. chemotherapy and immune prophylaxis. 3. immune response of infected organism. perspectives of malaria prevention]. 19862423092
humoral antibody response against bacteroides gingivalis-specific antigen recognized by monoclonal antibody in adult periodontal patients.an antigen recognized by monoclonal antibody specific for bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. the purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and silver staining, and the pattern of sds-page agreed with that of immunoblotting. the molecule exhibited an apparent molecular weight of about 62,000 by sds-page. the antigen was sensitive ...19862426200
differential expression of class ii (dr & dq) antigens by human gingival langerhans' cells and keratinocytes in vitro.the expression of the class ii products dr and dq on human gingival epithelium was examined using immunofluorescence and immunoperoxidase staining. differential expression of class ii antigens was seen in chronic gingivitis in adults, with t6(+) dr(+) cells being more numerous than t6(+) dq(+) cells. the periodontopathic organism fusobacterium nucleatum (fn) induced dq expression on langerhans' cells (lc) during in vitro explant culture of gingival tissue. this effect was mimicked by endotoxin ( ...19872435875
possible existence of a novel amphipathic immunostimulator in the phenol-water extracts of mycobacteriaceae.the extracts having diverse immunostimulating activities were obtained as a water-phase fraction from four bacterial species representing the 4 genera (mycobacterium, nocardia, gordona, and rhodococcus) of mycobacteriaceae by the phenol-water method, which is commonly used for extraction of endotoxic lipopolysaccharides (lps) from gram-negative bacteria and amphipathic substances from gram-positives. these fractions, especially those of g. aurantiaca and r. terrae, showed strong stimulatory effe ...19872441233
transient outward currents and changes of their gating properties after cell activation in thrombocytes of the newt.1. the electrical properties of the cell membrane of thrombocytes in the newt, triturus pyrrhogaster, were studied using the whole-cell variation of the patch-electrode voltage-clamp technique. 2. in medium containing ca2+ (1.8 mm), activated thrombocytes became round and then spread on the glass. activation of thrombocytes was inhibited by the removal of external ca2+ and addition of 1 w/v% albumin to the external media. 3. for thrombocytes kept in the resting state, depolarizations more positi ...20082443665
conformations of intermediates in the folding of the pancreatic trypsin inhibitor.intermediates in the folding pathway of the bovine pancreatic trypsin inhibitor (pti) have been examined by 1h nuclear magnetic resonance (n.m.r.). the intermediates were trapped during the reoxidation and consequent refolding of reduced pti by alkylating free thiols; each intermediate contained different disulphide linkages. the n.m.r. spectra reveal that conformational features of the native protein are present in the intermediate containing just one of the three normal disulphide linkages (30 ...19872443711
ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.deoxyribonucleic acid from 41 different bacterial species (including gram-negative and gram-positive species) were cleaved by different restriction endonucleases, electrophoresed in agarose and transferred to nylon filters. the dna fragments carrying rrna genes (rdna) were localized by hybridization with a 32p-labelled escherichia coli 16 + 23s rrna probe. a pattern of hybridized fragments was obtained for each dna tested. within a bacterial species (defined as a dna hybridization group), one or ...19862446639
respiratory syncytial virus fusion glycoprotein: further characterization of a major epitope involved in virus neutralization.competition experiments and biological assays with a panel of 15 monoclonal antibodies confirmed the presence of at least four antigenic sites on the fusion protein of human respiratory syncytial virus, three of which were involved in virus neutralization. one antigenic site, recognized by two strongly neutralizing antibodies, was conserved after reduction and denaturation and shown by immunoblotting to be localized on the f1 fragment of the fusion protein. cleavage of this protein with staphylo ...19872446731
the effects of nitrous oxide on p300 and reaction time.this experiment investigated the effects of 3 concentrations (15%, 25% and 35%) of nitrous oxide (n2o) on reaction time (rt) and p300 latency and amplitude. ten subjects responded to visually presented male or female names in an oddball paradigm with accuracy controlled at a high level. the results were analysed on a single-trial basis. nitrous oxide increased both rt and p300 latency in a dose-related manner and these variables showed a strong between-dose correlation (r = 0.67). nitrous oxide ...20082446836
spatial resolution of neuromagnetic records: theoretical calculations in a spherical model.spatial resolution of magnetoencephalography (meg) was studied by computer simulations using a spherical conductor model for the head. the accuracy obtainable in the absolute location of a dipole was found by calculating the confidence limits for source location in 3 dimensions. the accuracy in determining the relative locations of two sources was estimated by calculating the smallest shift in source location that could be detected with statistical significance. the results were used to illustra ...20082446847
[biochemical studies on peripheral nervous system]. 20072447920
identification of a galactose-binding lectin on fusobacterium nucleatum fn-2.a previous study has suggested that fusobacterium nucleatum fn-2 contains a galactose-binding protein (lectin) on the cell surface (p. a. murray, v. matarese, c. i. hoover, and j. r. winkler, fems microbiol. lett. 40:123-127, 1987). in the present study, the molecular specificity and size of this lectin were investigated by several techniques. whole-cell affinity chromatography with asialofetuin covalently coupled to sepharose 6mb demonstrated that 81% of 3h-labeled f. nucleatum were specificall ...19882451642
[immunochemical study of the lipopolysaccharides of yersinia enterocolitica serovars o5 and o5.27].y. enterocolitica lipopolysaccharides (lps), serovars o5 and o5.27, have similar antigenic specificity. the lps of serovar o5.27 has been shown to contain no factor o27. residual alpha-l-rhamnose, glycosylated by d-xylulose, has been found to play an important role in the formation of factor o5, common for both serovars.19882469272
characterization of coaggregation between bacteroides gingivalis t22 and fusobacterium nucleatum t18.bacterial adherence is a key factor in the colonization of the oral ecosystem, yet little is known about the mechanisms by which the pathogen bacteroides gingivalis adheres in the periodontal environment. we examined the ability of strains of b. gingivalis to coaggregate with selected microorganisms isolated from the subgingival microbiota of the cynomolgus monkey. a strong interaction was demonstrated between strains of b. gingivalis and fusobacterium nucleatum, whereas less pronounced or no in ...19892478473
ovine and bovine abortion associated with fusobacterium nucleatum. 19892488355
[chemiluminescence response and phagocytic activity of murine polymorphonuclear leukocytes to various species of oral bacteria].polymorphonuclear leukocytes (pmn) are predominant cells in the gingival crevice and saliva, and may play an important role in oral bacteria. murine peritoneal pmn was used and stimulated with 9 genera, 17 species of oral bacteria, including cariogenic and periodontal pathogens. the pmn response to the bacteria was measured by the luminol mediated chemiluminescence (cl) response and phagocytic activities, and the activities of lysozyme in the reaction medium after the cl response were also measu ...19892489336
hemocyte monophenol oxidase activity in mosquitoes exposed to microfilariae of dirofilaria immitis.monophenol oxidase (mpo) activity in hemocytes collected from aedes aegypti liverpool strain and aedes trivittatus intrathoracically inoculated with saline alone, inoculated with dirofilaria immitis microfilariae (mff), or from uninoculated mosquitoes was compared using a radiometric tyrosine hydroxylation assay. hemocyte mpo activity in mff-inoculated (= immune-activated) mosquitoes was significantly increased at 24 hr postinoculation (pi) in a. aegypti and at 6, 12, and 24 hr pi in a. trivitta ...19892493084
[flumazenil]. 20082493168
[teratogenic effect of thiotepa]. 20082493184
intravenous fat emulsion acutely suppresses neutrophil chemiluminescence.the immediate effect of intravenous fat emulsion on neutrophil oxidant release was studied. opsonized nonencapsulated s. aureus was used to stimulate neutrophil activity. luminol enhanced chemiluminescence was followed over 15 min and recorded as peak output (p; mv), integral under the curve (i; v-sec) and rate of increase (r; mv/sec). eighteen chronically ill patients receiving glucose based total parenteral nutrition were studied before and after a 4- to 6-hr test infusion of 500 ml of 10% fat ...20082515308
ethics in the nursing curriculum for all levels of education: baccalaureate, masters' and doctoral. 20082516597
[multiple sclerosis in health care in our country: electrophysiological, immunological and clinical analysis].among 70 patients referred for suspected multiple sclerosis (ms) a total of 40 met criteria for definite (17), likely (12) or possible (11) diagnosis of the disease. visual evoked potentials provided evidence of cns lesion in 13 cases. abnormalities of somatosensory evoked potentials, particularly on stimulation of the posterior tibial nerve, were present in 90% of patients. brainstem auditory evoked potentials were of little use, contributing to diagnosis of one subclinical lesion; they were no ...20082519355
[central pontine myelinolysis, associated with hyperglycemia and hyponatremia: a clinical case].a 68 year old diabetic male was admitted with hyponatremia and developed a "deafferentiation" syndrome. central pontine myelinolysis was suspected after clinical, eeg and brain cat scan. the diagnosis was confirmed at necropsy. the pathogenesis, clinical manifestations and treatment of this disorder are discussed.19892519379
[diagnosis of obesity: psychological aspects]. 20082519405
bactericidal concentrations of chlorhexidine-digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times.in vitro inhibitory and bactericidal concentrations in serum of chlorhexidine-digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against bacteroides gingivalis, bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and capnocytophaga sputigena. the minimal inhibitory concentration was assessed by the agar dilution technique. the killing curves and minimal bactericidal concentration of the antim ...19892524581
sidewinder retention sutures for closure of the abdominal wall.reported herein, a technique of sutures placement that avoids certain objections to the traditional style of retention sutures is described. it is particularly useful in patients undergoing repeated laparotomy and in patients whose wounds are closed in a layered manner and who are at high risk for dehiscence.20082525819
[gene cloning of an actinobacillus actinomycetemcomitans y4 antigen that reacts to peripheral blood sera in advanced destructive periodontitis patients].actinobacillus actinomycetemcomitans (a.a.) has been implicated in the etiology of juvenile periodontitis and also of advanced destructive periodontitis (adp). it has been reported that the levels of the igg antibody against the a.a. in the peripheral blood sera of the adp patients were often high as well as those against the bacteroides gingivalis. to clone the genes of antigen reactive to the sera of the adp patients, we constructed a phage library of the a.a. strain y4 dna in lambda l47, whic ...19892533606
lectinlike interactions of fusobacterium nucleatum with human neutrophils.fusobacterium nucleatum expresses lectinlike adherence factors which mediate binding to a variety of human tissue cells. adherence is selectively inhibited by galactose, lactose, and n-acetyl-d-galactosamine. in this study, adherence of f. nucleatum to human peripheral blood polymorphonuclear neutrophils (pmns) was investigated. the results indicated that the fusobacteria adhered to live and metabolically inactivated or fixed pmns. adherence of f. nucleatum resulted in activation of pmns as dete ...19892553609
unlikely association between clinically apparent herpesvirus infection and coronary incidence at older ages. the framingham heart study.experimental studies in chickens have shown a relationship of a herpesvirus to atherosclerosis. the hypothesis of an association in humans was tested by using data on the history of cold sores and other manifestations of herpes infections reported by 658 male and 919 female participants (ages 58 to 89) in the framingham heart study from 1977 to 1979 and on the prevalence and subsequent 6-year incidence of coronary heart disease (chd). approximately 40% of the men and 52% of the women reported a ...19892556099
absorption of human growth hormone from the rat lung.recombinant methionyl human growth hormone (hgh) was administered intratracheally to adult rats, and serum concentrations of immunoreactive hgh were measured for up to 24 h. the mean absolute bioavailability was approximately 36% after 18h and was similar for doses of 0.75, 1.5, and 3 mg/kg. peak serum hgh concentrations occurred at approximately 6 h after dosing. tritiated hgh (3h-hgh) was used to follow the clearance of hormone from the lungs. disappearance was linear with time and by 24 h app ...19892562650
treatment of mouse thymus cells with phosphatidylinositol-specific phospholipase c preferentially abrogates their reactivity with autoantibodies to the thy-1 antigen.thy-1 molecules have been shown to become anchored to the membrane of thymocytes and of t-cells via a phosphatidylinositol link. in the present study the intensity of immunofluorescent staining of mouse thymus cells by monoclonal xenoantibodies and alloantibodies specific for the thy-1.2 determinant was significantly reduced following exposure of the cells to phosphatidylinositol-specific phospholipase c (pi-plc). the majority of the pi-plc-treated thymus cells retained, however, some reactivity ...19892563957
utilization of amino acids and peptides by fusobacterium nucleatum.fusobacterium nucleatum strains atcc 10953, fevl, f1, f3, and f6 utilized amino acids, in particular glutamate, histidine, and aspartate were common to all strains. strain differences were observed in the utilization of threonine, serine, lysine, tyrosine, and methionine, and only strain atcc 10953 utilized all these amino acids. the glutamate and histidine pools were in all cases fully depleted before the other amino acids were attacked and at the same time all strains except 10953 started to u ...19892565597
drug advertising. 20082566076
first assisting in cataract surgery: program results.1. this program was established to teach non-physicians to first assist in cataract surgery, since third party payors would no longer pay for physicians to first assist. 2. among the advantages to utilizing non-physician first assistants are: availability, reliability, familiarity with instruments and equipment, and quality of service. 3. the questionnaire results confirm that the advantages of having non-physician first assistants far outweigh the disadvantages.20082566208
diabetes and schizophrenia. 20082574803
recognition of fusobacterium nucleatum subgroups fn-1, fn-2 and fn-3 by ribosomal rna gene restriction patterns.dna from representative strains of fusobacterium nucleatum subgroups fn-1, fn-2 and fn-3 was digested with restriction enzymes ecori and taqi and the electrophoretically separated fragments hybridized with a 32p-16s rrna gene probe from e. coli. the rrna gene restriction patterns from dna digested with either enzyme allowed the clustering of strains into the three subgroups. however, taqi digested dna yielded a wider distribution of taxonomically useful bands (ca 0.65 +/- 14.3 kbp) and the patte ...19892575556
the process of baculovirus deproteinization.the localization and function of alkaline protease inside the supervirion capsids (granules, inclusion bodies) of granulosis virus and nuclear polyhedrosis virus have been determined with the help of electron microscopy. it has been shown that deproteinization of baculoviruses proceeds in several steps. the first two stages (the release of virus from supervirion capsids and the release of nucleocapsids from the internal and external coats, were accomplished in the intestine of the insects, while ...19892576597
lysis of lymphoma cells by autologous and allogeneic natural killer cells.studies were undertaken to determine whether natural killer (nk) cells would lyse autologous and allogeneic lymphoma cells. when large granular lymphocytes, which are known to mediate nk activity, were enriched from peripheral blood and used as effector cells, they lysed autologous lymphoma cells of all of eight patients tested, and those of healthy donors lysed lymphoma cells of all of ten patients tested. the addition of interferon to the culture medium enhanced their cytotoxicity in three of ...20082578838
self reactive delayed type hypersensitivity (dth) induced in mice by syngeneic lymphoblasts.normal and x-irradiated a mice injected with syngeneic concanavalin a (con a)-induced lymphoblasts revealed after challenge with syngeneic lipopolysaccharide (lps)-induced lymphoblasts delayed type hypersensitivity (dth) measured both by footpad swelling and by 125iudr accumulation. mice injected with allogeneic con a-induced lymphoblasts and challenged with syngeneic lps-induced lymphoblasts or vice versa, also generated an appreciable dth response. in contrast, con a-induced blast cells of hum ...19852580776
leukocyte tubuloreticular inclusions in reye's syndrome.tubuloreticular inclusions (tri) have been observed in the rough endoplasmic reticulum of blood lymphocytes and monocytes in two cases of reye's syndrome initiated by influenza infections. tubuloreticular inclusions are seen in these mononuclear leukocytes during the acute phase of illness, but not during convalescence. since tri have been demonstrated in peripheral mononuclear leukocytes in patients with acquired immunodeficiency syndrome, systemic lupus erythematosus, and certain viral infecti ...19852581524
lipid keratopathy in rabbits. an animal model system.rabbits rendered hypercholesterolemic on a high cholesterol diet while subjected to corneal suture placement to induce neovascularization made useful models for qualitatively and quantitatively studying lipid keratopathy. forty rabbit eyes were subjected to placement of four sutures located between 1 and 4 mm from the limbus. the neovasculature grew at a constant rate of 0.24 mm/day, unaffected by serum cholesterol levels. rabbits fed cholesterol prior to surgery were the earliest to display lip ...19852581537
lateralization of the hoffmann reflex from the long flexor thumb muscle in right- and left-handed normal subjects. 20082583947
recovery from coma that results as a complication of cardiac arrest followed by cardiopulmonary bypass.many possible complications are associated with cardiopulmonary bypass. they are similar to the risks accompanying most surgical procedures and include stroke, renal failure, and death. this potential for complication increases when bypass exceeds 2 hours and rises sharply when pump time is prolonged more than 3 to 4 hours. one group of serious complications is major neurologic disorders. the risk of significant cerebral dysfunction, that is, severe focal stroke or coma, is about 1%, and this ri ...20082584045
characterization of coagulase-negative staphylococci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses.coagulase-negative staphylococci are important nosocomial pathogens. at present, no wholly satisfactory typing scheme exists for these organisms. therefore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting were assessed as characterization methods. a total of 100 type strains and nontyped isolates representing nine species of coagulase-negative staphylococci were analyzed. each species had a reproducible, characteristic whole-cell banding pattern when analy ...19892584373
differential expression of the il-2 receptor subunits, p55 and p75 on various populations of primary peripheral blood mononuclear cells.unstimulated pbl were examined for expression of il-2r subunits, il-2rp55 and il-2rp75, by two-color flow cytometric analyses using mab. nkh-1+ non-t non-b cells expressed il-2rp75 but not il-2rp55, and the il-2rp75 sites on purified nkh-1+ cells were determined to be 1630 sites/cell by binding of 125i-labeled tu27 mab specific for il-2rp75. in the cd4+ t cell population, il-2rp55+ cells were significantly detected, but little or marginally of the il-2rp75+ cells. however, il-2rp75+ cells were s ...20082584707
not for specialists only--caring for patients with spinal cord injuries. 20022586900
discipline without punishment--part i. 19992586930
[changes in lymphocytes in acute suppurative-destructive lung diseases].a study of the content of lymphocytes and their populations, indices of intracellular viral involvement of the lymphocytes in 32 patients with acute purulent-destructive pulmonary diseases and 46 healthy persons revealed an increase of the percent of non-viable lymphocytes containing viral inclusions. viral involvement of lymphocytes is considered one the leading causes of increased lymphocyte death.20082588523
[a clinicopathological study of recurrent gastric cancer cases treated by a resection of the remnant stomach].seventeen cases of a recurrent gastric cancer that were treated by a resection of the remnant stomach have been studied retrospectively. for first management 12 cases were given a billroth 2 procedure, 4 cases a billroth 1 procedure and 1 case a fundectomy. for the recurrent cancer, 13 cases were given a total resection of the gastric remnant and 4 cases a partial resection. the overall mortality rate was 12% and the survival rate was 58% at 1 year, and 8% at 5 years. we thus have concluded that ...20082593232
complications and inappropriate use of minitracheostomy. 20082596687
[inducing effect of periodontopathic bacteria on activation of macrophage functions and production of interleukin-1 by mouse peritoneal macrophages].periodontal disease is thought to be initiated by a bacterial infection and subsequently developed by immunopathological mechanisms thorough host-parasite interactions. the macrophage and lymphocyte are the major functional cell types in the lesion of the disease and participate in tissue destruction and alteration of the periodontal connective tissue as well as in host defense mechanisms. however, the detailed implications of macrophages in development of the disease is still unclear. the aim o ...19892601596
management of ingrowing toenail. 20082605481
cytologic and bacteriologic evaluation of tracheobronchial aspirates from clinically normal foals.thirty-eight tracheobronchial aspirates (tba) were collected from twenty 1 to 6-month-old foals, which were free of clinical signs of respiratory tract or other infectious disease. we collected tba from 9 of the foals 3 times when they were approximately 8, 16, and 24 weeks old. aspirates were examined cytologically after staining with modified wright-giemsa, gram, toluidine blue, and prussian blue stains. aerobic bacterial culturing was performed on all aspirates. of the 20 initial tba, 4 (20%) ...19892610430
cytologic and bacteriologic evaluation of tracheobronchial aspirates from clinically normal foals.thirty-eight tracheobronchial aspirates (tba) were collected from twenty 1 to 6-month-old foals, which were free of clinical signs of respiratory tract or other infectious disease. we collected tba from 9 of the foals 3 times when they were approximately 8, 16, and 24 weeks old. aspirates were examined cytologically after staining with modified wright-giemsa, gram, toluidine blue, and prussian blue stains. aerobic bacterial culturing was performed on all aspirates. of the 20 initial tba, 4 (20%) ...19892610430
[analysis of the nursing factors responsible for expanding the sphere of daily activities of patients with parkinson's disease]. 20082612141
[analysis of the nursing factors responsible for expanding the sphere of daily activities of patients with parkinson's disease]. 20082612141
fifty hours for the poor? 20082612144
fifty hours for the poor? 20082612144
nile red fluorescence response from the aortic arch to serum cholesterol changes.histologic sections of the aortic arches from guinea pigs were stained with nile red to verify the possibility of quantitating the earliest changes in the arterial wall. nile red fluorescence intensity was quantitated by conversion to electric signal. fluorescence in a group fed cholesterol and casein was significantly higher than that in a control group. its dependence on the logarithm of serum cholesterol concentrations between 1.33 and 7.67 mmol/l was found. small fluorescence intensity diffe ...19892612173
nile red fluorescence response from the aortic arch to serum cholesterol changes.histologic sections of the aortic arches from guinea pigs were stained with nile red to verify the possibility of quantitating the earliest changes in the arterial wall. nile red fluorescence intensity was quantitated by conversion to electric signal. fluorescence in a group fed cholesterol and casein was significantly higher than that in a control group. its dependence on the logarithm of serum cholesterol concentrations between 1.33 and 7.67 mmol/l was found. small fluorescence intensity diffe ...19892612173
high-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis.selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. after addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase c18 bond-elut columns and from urine using pre-packed silica extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before inj ...19892613803
[clinical study of osteoporosis]. 20082614196
participation of complement components in glomerular deposition in iga nephropathy.to clarify the role of complement components in glomerular deposition in iga nephropathy, clinicopathological and immunohistological studies were performed on 299 patients (171 males and 128 females; age, 9-71 years). glomerular iga deposition with igg and/or igm was observed more frequently in patients with clq and/or c4 than in those with only c3 deposition (p less than 0.001). patients with glomerular deposition of clq and/or c4 showed more severe proteinuria (1 g/24 hr less than), a lower gl ...20082615014
[distribution pattern and risk factors of lung cancer in qidong rural area].distribution pattern, epidemiologic trend and risk factors of lung cancer in rural area were studied and analysed based on the data of qidong cancer registry (1972-1986), of sampling survey of cigarette smoking rate among rural residents, and of consumption of cigarette and fuel in qidong. the results showed that: 1. lung cancer mortality had increased from 9.00/10(5) in 1972 to 20.03/10(5) in 1986 with annual increase rate of 5.88%; 2. lung cancer mortality in 1980s (1984-1986) was increased by ...20092620633
the effects of acetyl-l-carnitine on experimental models of learning and memory deficits in the old rat.experimental models of learning and memory deficits in aged rats can be studied by means of behavioural tests that provide an important tool for evaluating the effect of drugs on these parameters. active and passive avoidance tests showed a clear impairment of learning and memory capacity of old rats. these tests were also used to study the behavioural effect of acetyl-l-carnitine in aged rats. the subchronic treatment with this drug was followed by a significant improvement of acquisition and r ...19892620857
the effects of acetyl-l-carnitine on experimental models of learning and memory deficits in the old rat.experimental models of learning and memory deficits in aged rats can be studied by means of behavioural tests that provide an important tool for evaluating the effect of drugs on these parameters. active and passive avoidance tests showed a clear impairment of learning and memory capacity of old rats. these tests were also used to study the behavioural effect of acetyl-l-carnitine in aged rats. the subchronic treatment with this drug was followed by a significant improvement of acquisition and r ...19892620857
standardisation of quantitative direct gas liquid chromatography for early detection of bacteria in blood cultures.blood cultures with strains of aerobic, facultative and obligate anaerobic bacteria were studied by quantitative direct gas liquid chromatography for early diagnosis of bacteraemias. small amounts of volatile and nonvolatile fatty acids were detected in uninoculated blood cultures. bacteroides fragilis produced acetic (27.6 mumol/ml), propionic (1.0 mumol/ml), isovaleric, (0.6 mumol/ml), lactic (4.5 mumol/ml) and succinic (2.7 mumol/ml) acids after 48 h. blood cultures inoculated with clostridiu ...19892620945
interleukin-1 and interleukin-1 inhibitor production by human adherent cells stimulated with periodontopathic bacteria.this study examined the effect of the putative periodontopathic bacteria bacteroides gingivalis and fusobacterium nucleatum on the production of interleukin-1 (il-1) and il-1 inhibitors by human plastic-adherent mononuclear cells from normal donors. fusobacterium mortiferum was used as a non-oral, non-pathogenic control organism. unstimulated adherent cells spontaneously secreted an il-1 inhibitor, whereas stimulation with b. gingivalis induced the synthesis and secretion of il-1. with both fuso ...19892624558
outer membrane proteins as major antigens of fusobacterium nucleatum.the immunochemical reactions of rabbit polyclonal antibodies directed to different preparations of fusobacterium nucleatum i.e, whole cells, peptidoglycan associated proteins, a peptidoglycan-protein complex and a purified 40 kilodalton (kda) protein, were investigated on outer membrane preparations of fusobacterium species and a restricted number of leptotrichia buccalis after their separation on sodium dodecyl sulphate polyacrylamide gels and electrotransfer to nitrocellulose. all f. nucleatum ...19892627499
[myopathy in the uremic patient: apropos of a case].uremic myopathy is relatively unknown, despite the fact that it can be quite a severe handicap. it is a non-specific proximal myopathy which must be distinguished from uremic polyneuropathy. several disorders linked to renal insufficiency have been implicated, but these only play an adjuvant role with regard to renal osteodystrophy. indeed the presence of myopathy in cases of severe osteodystrophy, its similarity to the myopathies associated with different types of osteomalacia and hyperparathyr ...20082633067
[endomyocardial fibrosis]. 20082633945
[endomyocardial fibrosis]. 20082633945
narcolepsy in a long-haired dachshund.narcolepsy was diagnosed in a three-month old, longhaired dachshund presented with a history of sudden onset of episodes of complete collapse. littermates or immediate ancestors were not affected. numerous daily cataplectic attacks as well as excessive sleepiness were the main clinical features. the dog did not respond to treatment with methylphenidate and imipramine. the clinical signs of the disease disappeared after treatment with dexamphetamine. this report describes a case presentation, fin ...19892634775
tandem neurofibromas associated with cord compression. 20092635053
[measurement of orthodontic appliance force using prototype sensors].in introductory remarks the most important biomechanical principles, which are relevant for the research, and conditions which must be satisfied presented in intraoral measuring of orthodontic forces were pointed out. first the aim of the research was defined, and then the investigation of mechanical properties of acrylics as a material for orthodontic appliances were carried out. the type of strain gauges, bonds and measuring system configurations, as well as measuring amplifiers and recording ...20082635572
[vestibular evoked potentials in "gallus domesticus"].electrophysiological activity in response to linear acceleration stimuli was recorded from gallus domesticus by means of subcutaneous electrodes. this investigation had two purposes: 1) to obtain normative data for our laboratory, and 2) to rule out auditory and somatosensory contributions to the vestibular evoked potentials (vsep). the stimulus consisted of a sigmoid-shaped voltage function generated by a digital-to-analog converter. this signal was amplified, attenuated, and directed to a shak ...19892635851
purification and partial characterization of a major outer-membrane protein of fusobacterium nucleatum.the major outer-membrane proteins of 40-41 kda were identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) in fusobacterium nucleatum strains atcc 10953, atcc 25586, f3, f6 and fev1. the proteins were purified by preparative gel electrophoresis. their behaviour in gel filtration and gel electrophoresis, their sensitivity to proteolytic enzymes, and their amino acid composition were investigated. the purified proteins were partly sequenced from the n-terminal end. a 3 ...19892636259
[essential arterial hypertension in patients with non-insulin-dependent diabetes mellitus during and after balneotherapy followed-up for 6 years].the statistical correlations between body weight, glycemia, total lipids, cholesterol in 200 patients with essential hypertension, especially borderline essential hypertension, followed up for 6 years indicate that the complex environmental factors in sl─ânic-moldova health resort associated with balneotherapy may induce a return to normal values of the above mentioned parameters. good results were also obtained in the patients with essential hypertension and non-insulin dependent diabetes mellit ...20092636767
layer thickness in rotational panoramic radiography: some specific aspects.it is customary to define the thickness of the image layer in rotational panoramic radiography along the central ray of the x-ray beam. this results in calculated values which overestimate its thickness, especially in regions where the central ray deviates markedly from orthogonality. a supplementary definition is proposed which considers layer thickness in a direction perpendicular to the central plane of the image layer. this provides values which may be more easily related to the thickness of ...20092637877
[effects of periodontopathic bacterial components on phagocytic activity of rat peritoneal macrophages. examination using elisa].this study examined the phagocytic activity of rat peritoneal resident macrophages to determine the movement of macrophages in local inflammation in periodontal disease. we studied phagocytic activity by enzymelinked immunosorbent assay (elisa) and used the peroxidase-anti-peroxidase soluble complex (pap; soluble immune complex) as a marker in. we also determined the basic conditions of this examination and studied the effects of bacterial components and the supernatants of sonicated periodontop ...19892637908
"reverse" dna hybridization method for the rapid identification of subgingival microorganisms.a "reverse" hybridization method is described, in which whole chromosomal dna was extracted from 10-20 colonies of "unknown" strains in pure culture and labelled with digoxigenin by a random primer technique. dna probes were prepared from a total of 23 strains and hybridized with targets containing 100 ng purified, denatured dna from 38 reference strains fixed to nitrocellulose. 21/23 digoxigenin-labelled dna probes successfully detected all members of the homologous species present on filters. ...19892639298
cytotoxicity against various cell lines of lipopolysaccharides purified from bacteroides, fusobacterium, and veillonella isolated from infected root canals.the cytotoxicity against two mesenchymal cell lines, l-929 and wi-38, and two epithelial cell lines, kb and hela s-3, of lipopolysaccharide (lps) purified from strains of bacteroides gingivalis, fusobacterium nucleatum, and veillonella parvula isolated from infected root canals was investigated. the inhibition of cell growth by these lps's was considerable for mesenchymal cell lines, but mild for epithelial cell lines. the cytotoxic effect of f. nucleatum lps was the greatest and that of b. ging ...19892639950
effects of periodontopathic bacteria on il-1 and il-1 inhibitor production by human polymorphonuclear neutrophils.the effect of heat-killed periodontopathic bacteria on the production of interleukin-1 (il-1) and an il-1 inhibitor by human polymorphonuclear neutrophils (pmn's) was examined. peripheral blood was obtained from 18 healthy volunteers and the pmn's were separated using dextran sedimentation and ficoll-paque density gradient centrifugation. the pmn's (5 x 10(5) cells/well) were cultured in serum-free media with or without heat-killed periodontopathic bacteria. four gram-negative periodontopathic b ...19892640313
[studies on anaerobic infection in oro-maxillary region--rapid diagnosis by gas-liquid chromatography and antibiotic susceptibilities of anaerobic bacteria].subject material for this study was pus collected from patients with purulent inflammation in the oro-maxillary region. direct gas-liquid chromatography (glc) analysis was made, bacterial isolation and identification were carried out, and comparisons were made with results from glc analysis and anaerobic isolates in a pyg medium. in addition, antibiotic susceptibilities of anaerobic bacteria were examined. results 1. anaerobic bacteria were isolated from 85 of 100 cases of obstructive abscesses. ...19892641196
comparison of the long-term, postsurgical survival of women and men in the coronary artery surgery study (cass).this study compares the survival of men and women an average of 6 years after coronary artery bypass graft surgery (cabg) by means of the coronary artery surgery study (cass) registry. subjects included in these analyses were the 6100 men and 1097 women who survived surgery. medical history and physical and laboratory information were collected from each patient at baseline. men and women were compared for differences in baseline characteristics, long-term survival (by means of the cox proportio ...20062643286
preventing the kidney disease of diabetes mellitus: public health perspectives. 20072643304
structural analysis of lipopolysaccharides from eikenella corrodens by use of murine monoclonal antibodies.the structure of lipopolysaccharides (lpss) of eikenella corrodens was analyzed with prepared murine monoclonal antibodies. a common core epitope was found in three of seven lps preparations from e. corrodens strains and fusobacterium nucleatum atcc 25586. four e. corrodens lpss were found to possess an o-side-chain epitope which cross-reacted with lpss from fusobacterium necrophorum atcc 25286 and capnocytophaga ochracea m-12. lipid a of e. corrodens lps shared an epitope common among lpss from ...19892643582
effects of cyclosporin a on gingival status following liver transplantation.cyclosporin a, since its introduction, has drastically reduced the morbidity and mortality rates associated with organ transplants. one side-effect of csa is gingival hyperplasia. this study investigated the effects of csa given in conjunction with a low dose of steroids on the gingival status of pediatric patients following liver transplantation. a total of 21 such patients were compared with 23 healthy children, matched for age and sex. data collected included csa serum levels, gingival width, ...20082643647
effects of cyclosporin a on gingival status following liver transplantation.cyclosporin a, since its introduction, has drastically reduced the morbidity and mortality rates associated with organ transplants. one side-effect of csa is gingival hyperplasia. this study investigated the effects of csa given in conjunction with a low dose of steroids on the gingival status of pediatric patients following liver transplantation. a total of 21 such patients were compared with 23 healthy children, matched for age and sex. data collected included csa serum levels, gingival width, ...20082643647
chemiluminescence of peripheral polymorphonuclear leukocytes from adult periodontitis patients.polymorphonuclear leukocytes (pmn's) constitute a primary host resistance factor against infection. this study investigated the chemiluminescent (cl) response of peripheral blood pmn's isolated from human subjects with adult periodontitis. 32 subjects were categorized on the basis of age and periodontal disease status into 4 equal groups--young healthy, young diseased, old healthy and old diseased. pmn cl was stimulated using heat-killed, serum-opsonized fusobacterium nucleatum--a specific perio ...19892646331
central pontine myelinolysis after liver transplantation.eight adults and 3 children out of 85 patients who had neuropathologic examination after death following orthotopic liver transplantation showed central pontine myelinolysis (cpm). four patients also had extrapontine myelinolysis. eight patients had significant serum sodium changes. in 5, the fluctuation occurred perioperatively and 4 had a clinical picture consistent with cpm, although no patient had this as an antemortem diagnosis. we emphasize the role of hepatic dysfunction as a cause of cpm ...20082648187
central pontine myelinolysis after liver transplantation.eight adults and 3 children out of 85 patients who had neuropathologic examination after death following orthotopic liver transplantation showed central pontine myelinolysis (cpm). four patients also had extrapontine myelinolysis. eight patients had significant serum sodium changes. in 5, the fluctuation occurred perioperatively and 4 had a clinical picture consistent with cpm, although no patient had this as an antemortem diagnosis. we emphasize the role of hepatic dysfunction as a cause of cpm ...20082648187
the confined space. a review of a recently discerned medical entity.this paper presents an extensive review of the literature relating to the industrial confined space. it defines the various types of confined spaces, classifies their associated noxious atmospheres, and identifies the potential hazards of each. a newly recognized confined space, the environment of the surgical amphitheater in which the surgical patient undergoes general anesthesia, has been added. pathological alterations bearing upon these hazards and the forensic principles of confined space i ...20082648810
the confined space. a review of a recently discerned medical entity.this paper presents an extensive review of the literature relating to the industrial confined space. it defines the various types of confined spaces, classifies their associated noxious atmospheres, and identifies the potential hazards of each. a newly recognized confined space, the environment of the surgical amphitheater in which the surgical patient undergoes general anesthesia, has been added. pathological alterations bearing upon these hazards and the forensic principles of confined space i ...20082648810
[oncogenes in human leukemia].results of our study on the activation of n-ras oncogene by point mutation in human leukemia and myelodysplastic syndrome have been described in this article. point mutation was observed mainly on the 12th, 13th and 61st amino acid codon of ras genes. therefore, oligomers containing mutations at these codons were used as probes for dot blot analysis of dna derived from patient's bone marrow cells or leukemia cells. polymerase chain reaction technique was used to amplify the dna of ras genes cont ...19892650633
antineutrophil cytoplasm antibodies in kawasaki disease.autoantibodies against components of neutrophil cytoplasm develop during adult vasculitic diseases such as wegener's granulomatosis and microscopic polyarteritis, and they are predominantly of the igg class. similar but distinct antibodies have been described in children with kawasaki disease and both igm and igg class antibodies are represented. this adds another clinically distinct childhood form of vasculitis to the adult forms in which autoantibodies to neutrophil cytoplasmic antigens have b ...20082650637
new techniques for evaluating metabolic brain injury in newborn infants. 20082651005
anion exchange chromatographic separation of inositol phosphates and their quantification by gas chromatography.the direct measurement of mass of inositol trisphosphate from biologic samples is described. separation of inositol monophosphate, bisphosphate, trisphosphate, and inositol tetrakisphosphate was achieved using anion exchange chromatography with a sodium sulfate gradient. in addition, separation of the isomers of each inositol phosphate was performed using hplc procedures. the individual inositol phosphate fractions were subsequently dephosphorylated and desalted. the myo-inositol from each fract ...19982653097
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