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[the delta virus: the cuckoo's egg in the nest of hepatitis b virus]. 19863785445
sem studies of acellular glomerular basement membrane in human diabetic glomerulopathy.previous transmission electron microscopic studies have demonstrated glomerular basement membrane (gbm) thickening and mesangial matrix (mm) expansion in chronic stages of diabetes. it is difficult, however, to achieve an appreciation of gbm surface features and distribution of mm in planar views. in the current study, autopsy human renal cortical tissue from patients with end-stage diabetic nephropathy were minced and rendered acellular with detergents prior to fixation, cryofracture, and prepa ...19863789417
microenvironment of the enzyme-bound nadh is different in lobster and pig muscle glyceraldehyde-3-phosphate dehydrogenase microcrystals.two possible consequences of crystal lattice formation were studied with glyceraldehyde-3-phosphate dehydrogenases isolated from lobster (palinurus vulgaris) and pig muscle: changes in the microenvironment of the nadh-binding site as detected by fluorescence polarization, and differences in the maximal activities of the microcrystalline enzymes as compared to those in solution. in solution practically no difference was found between the polarization values of the enzyme-nadh and the catalytic in ...19863789735
survival of erythroid burst-forming units and erythroid colony-forming units in canine bone marrow cells exposed in vitro to 1 mev neutron radiation or x rays.conditioned media (cm) from allogeneic stimulated cultures of light density cells (less than 1.08 g/cm3) from the peripheral blood of normal dogs were used to stimulate the growth of erythroid burst-forming units (bfu-e) in bone marrow from normal dogs. maximum numbers of bfu-e were obtained when 5% (vol/vol) 3 x cm and 2 u/ml erythropoietin were added to plasma clot cultures of bone marrow cells. in addition, the radiation sensitivity (d0 value) was determined for cfu-e and for bfu-e in bone ma ...19863797639
didemnin b--an immunosuppressive cyclic peptide that stimulates murine hemagglutinating antibody responses and induces leukocytosis in vivo.didemnin b (db) is a cyclic peptide with potent immunosuppressive activity in vitro and in the murine graft-versus-host-reaction (gvhr), the only measure of in vivo immunity tested in our prior studies. because continued production of mature leukocytes by bone marrow and an intact antibody response are crucial to defense against infection in immunosuppressed patients, we have evaluated the effects of db on these processes as well. anti-sheep red blood cell (srbc) hemagglutinating antibody (hab) ...19873798555
[neuroblastoma; biology and prognostic factors].recently accumulated data on the biology of the tumors of neuroblastoma group are reviewed. histopathological classification system developed by shimada et al, elevated levels of serum neuron-specific enolase and ferritin, and gene abnormalities including partial deletion of the short arm of chromosome #1 and n-myc gene amplification are discussed as well as their prognostic significance. also, the current activities of ccsg (childrens cancer study group) neuroblastoma studies are briefly report ...19873800408
ehlers-danlos syndrome. a variant characterized by the deficiency of pro alpha 2 chain of type i procollagen.the clinical findings and biochemical studies are described for a 30-year-old man with ehlers-danlos syndrome. type analysis of collagen produced by cultured fibroblasts revealed the lack of a detectable pro alpha 2 chain of type i procollagen. the intracellular degradation rate of newly synthesized collagen was higher than that of normal cells, resulting in the reduction of net collagen production. clinical manifestations characterized by cardiovascular abnormalities due to decreased collagen d ...19873800425
head-space gas-liquid chromatographic analysis for presumptive identification of bacteria in blood cultures.a total of 445 strains of common aerobic and anaerobic bacteria were analyzed for their volatile microbial metabolites by head-space gas-liquid chromatography. the bacteria were incubated for 24 or 48 h in blood-containing trypticase soy or lombard-dowell glucose broth before being analyzed by automatic head-space injection. from the chromatographic pattern obtained, the majority of klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, escherichia coli, pseudomonas aeruginosa, morgan ...19863816354
[fertilization of human oocytes in vitro and embryo transplantation]. 19863817215
production of monoclonal antibodies that recognize specific and cross-reactive antigens of fusobacterium nucleatum.monoclonal antibodies (mabs) against the cell surface antigens of fusobacterium nucleatum 263 were obtained by fusion of murine myeloma cells (p3-nsi/1-ag4-1) with the splenocytes of balb/c mice immunized with whole cells of f. nucleatum 263. screening was performed using an enzyme-linked immunosorbent assay (elisa) against the immunizing strain, f. nucleatum 263. further selection was done using a bacterial panel consisting of bacteroides, actinomyces, streptococcus, fusobacterium, and escheric ...19873818097
effect on wool growth of thyroxine replacement in thyroidectomized merino rams.the effect on wool growth of thyroidectomy with subsequent thyroxine replacement at subnormal and supranormal levels has been investigated in merino rams fed a restricted basal diet. thyroidectomy without thyroxine replacement caused a greater than 60% reduction in wool growth. provision of 30% of normal plasma thyroxine concentrations was sufficient to return wool growth to normal. similarly, complete replacement of plasma thyroxine levels gave normal wool growth while increasing thyroxine conc ...19853834890
shigellosis in children--prevalence of subgroups and antibiotic resistance. 19853836974
inheritance of the human salivary proline-rich proteins: a reinterpretation in terms of six loci forming two subfamilies.dna studies suggest that six loci control the synthesis of human salivary proline-rich proteins (prps). genes at two of these loci (proposed names, prh1 and prh2) contain regions that strongly hybridize to a probe made from a cdna in which sites for the restriction enzyme haeiii occur repeatedly; they code for the acidic prps. genes at the remaining four loci (prb1, prb2, prb3, and prb4) contain regions that strongly hybridize to a probe with repeated bstn1 sites; they probably code for the basi ...19853840016
effects of a fusobacterium nucleatum extract on immunoregulation in mice.the purpose of this study was to examine the effect of an extract of fusobacterium nucleatum on humoral and cell-mediated immunity and phagocyte functions in mice. the extract was obtained from the heat-inactivated supernatant of sonicated f. nucleatum. mouse splenocytes were cultured with sheep red blood cells (srbc), and antibody formation was assayed by counting the plaque-forming cells (pfc). after c3h mouse skin was grafted onto the balb/c mouse, cytotoxicity of balb/c mouse splenocytes to ...19853855894
[estradiol hormone receptors in human gingiva: preliminary results]. 19853858256
survival analysis of periodontal sites before and after periodontal therapy.periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ...19853860518
gram negative species associated with active destructive periodontal lesions.apical subgingival plaque samples were taken from 19 subjects exhibiting active destructive periodontal disease. the predominant cultivable gram negative species from 50 active sites were compared to 69 inactive sites of comparable pocket depth and attachment level loss. active disease sites were chosen which showed a significant loss of attachment within a two-month interval. proportions of gram negative rods were higher in active periodontal disease sites than in inactive sites. species which ...19853863838
[bacterial endotoxin from fusobacterium nucleatum--particularly the role of endotoxin in periodontal diseases]. 19853864881
adhesion of anaerobic gram-negative bacteria to mucosal surfaces.fusobacterium nucleatum and black-pigmented bacteroides species adhere to red blood cells and crevicular epithelium. the attachment of the bacteroides, but not f. nucleatum, is associated with the presence of fimbriae-like structures on the bacterial surface. such structures have been observed also in unencapsulated bacteroides fragilis able to adhere to human red cells and cheek epithelium. encapsulated b. fragilis adheres to porcine brush borders, but the number of adhering cells per brush bor ...19853865351
intramembrane particle distribution on the plasma membrane of ruffle-ended and smooth ended maturation ameloblasts of the rat incisors.plasma membrane structure and intramembrane particle (imp) distribution in ruffle-ended (ra) and smooth-ended (sa) maturation ameloblasts were investigated by means of freeze-fracture replication of the rat incisor enamel organ. on the plasma membranes of both the lateral and distal cell surfaces, mean values of imps per 1 micron2 obtained from the sas were lower than that from the ras. this does not exclude the ameloblast modulation phenomenon but rather supports a radical shift of functional a ...19853865928
short-chain collagen genes and their expression in cartilage. 19853868942
reaction of human sera from juvenile periodontitis, rapidly progressive periodontitis, and adult periodontitis patients with selected periodontopathogens.the levels of serum antibody reactive to selected periodontopathogens were determined in 182 clinically characterized patients: 35 healthy control, 50 juvenile periodontitis, 42 adult periodontitis and 55 rapidly progressive periodontitis. reactive antibody levels were determined using an enzyme-linked immunosorbent assay with whole cell preparations of bacteroides gingivalis, capnocytophaga (bacteroides) ochraceus, fusobacterium nucleatum and actinobacillus actinomycetemcomitans (y-4) serving a ...19853869650
purification and properties of a novel beta-lactamase from fusobacterium nucleatum.a strain of fusobacterium nucleatum which produced high levels of beta-lactamase was isolated. the specific activity of the unpurified beta-lactamase was 7.8 u/mg of protein. by sephacryl s-300 and s-200 column passage and chromatofocusing, the enzyme was purified 450-fold. sodium dodecyl sulfate-gradient gel electrophoresis revealed a single band. the enzyme hydrolyzed phenoxymethylpenicillin, benzylpenicillin, and ampicillin more rapidly than carbenicillin and piperacillin. cephaloridine, cefa ...19853875311
early rheumatoid-like synovial lesions in rabbits drinking cow's milk. i. joint pathology.rheumatoid-like lesions developed in 9 of 25 (36%) old english rabbits drinking cow's milk for 12 weeks. the incidence of lesions in male and female animals was similar. the majority of rabbits drinking cow's milk developed increased numbers of nucleated cells and raised percentages of t lymphocytes in their synovial fluids, compared to control rabbits. the cell counts and t cell percentages correlated with the severity of the histological lesions. no evidence of glomerulonephritis was observed ...19853876289
characterization of a new beta-lactamase from fusobacterium nucleatum by substrate profiles and chromatofocusing patterns.seven beta-lactamase-producing fusobacterium nucleatum strains were isolated from patients with recurrent tonsillitis. the isolates were highly resistant to phenoxymethylpenicillin, benzylpenicillin, ampicillin, piperacillin and cloxacillin. they were all sensitive to cefaclor, cephaloridine, cefotaxime, cefoxitin, latamoxef (moxalactam) and imipenem. penicillinase activity was found intracellularly after 20 h incubation. specific activity of the unpurified penicillinase varied among the strains ...19853876327
absence of hepatic molybdenum cofactor. an inborn error of metabolism associated with lens dislocation.there are many causes of lens dislocation in man. amongst these are two inborn errors of sulfur amino acid metabolism, viz., homocystinuria and sulfite oxidase deficiency. to date nine patients have been found in whom a combined deficiency of sulfite oxidase and xanthine dehydrogenase was observed. this inherited disease is due to a defective synthesis of molybdenum cofactor, an essential component for the assembly of both enzymes. the main clinical symptoms of these patients were: facial dysmor ...19853877898
beta-lactamases in anaerobic bacteria.the known mechanisms of beta-lactam resistance in anaerobic bacteria involve production of beta-lactamases, alteration of penicillin-binding proteins and blocked penetration of beta-lactams through the outer membranes. the most important factor in beta-lactam resistance is production of beta-lactamase. beta-lactamases in various bacteroides, fusobacterium and clostridium species have been described. beta-lactam resistance in bacteroides fragilis is most commonly mediated by beta-lactamase produc ...19853877980
removal of marrow t cells with okt3-okt11 monoclonal antibodies and complement to prevent acute graft-versus-host disease. a pilot study in ten patients.we studied the feasibility of t cell depletion of bone marrow for transplantation in preventing acute graft-versus-host disease gvhd in patients having a high risk of acute gvhd. we report our preliminary clinical experience of the ex-vivo treatment of allogeneic marrow using a pan-t monoclonal antibody combination (okt3-okt11) plus baby rabbit complement. ten patients received allografts from hla-identical sibling donors. all patients had malignant hematological disease with poor prognosis (6 a ...19853881852
a newly recognized cause of travelers' diarrhea: enteroadherent escherichia coli.adherence to hep-2 tissue culture cells has been proposed as a virulence characteristic of enteropathogenic escherichia coli (epec). a preliminary study revealed that e. coli that adhered to hep-2 cells, but did not produce conventional enterotoxins and did not belong to recognized epec serogroups, could be isolated from adults from the united states who acquired diarrhea in mexico. the purpose of this study was to determine the prevalence of these enteroadherent e. coli (eaec) in 188 travelers ...19853882849
[a simple culture method for the bacteriological identification of bacillus larvae on columbia blood-slant agar]. 19853883984
[a simple culture method for the bacteriological identification of bacillus larvae on columbia blood-slant agar]. 19853883984
a procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver.experimental conditions have been designed that permit both extracellular and intracellular immunolocalization of various collagen types and fibronectin in rat liver. the procedure involves paraformaldehyde fixation by perfusion of the organ, use of saponin as a membrane permeabilizing agent, and visualization of the matrix components by indirect immunoperoxidase. intracellular demonstration of collagens was particularly sensitive to the composition of the fixative and the duration of fixation. ...19853886779
[rare forms of yersinia infections in adults]. 19853887018
establishment of mics of moxalactam for control and reference anaerobic organisms in agar dilution and microdilution techniques.the mics of moxalactam were determined for eight national committee for clinical laboratory standards control and reference strains and for fusobacterium nucleatum atcc 10953 by agar and microdilution techniques. the recommended mic for the control strain bacteroides fragilis in both agar and microdilution tests is 0.5 micrograms/ml. recommended mics for bacteroides thetaiotaomicron atcc 29741 and clostridium perfringens atcc 13124 by agar dilution are 8 and 0.063 micrograms/ml, respectively. th ...19853888103
b/t cell ratio of rabbit peripheral blood lymphocytes. influence of separation technique on results.spontaneous in vitro t cell rosette formation at room temperature leading to enrichment of b cells has been reported. we tested 10 individual new zealand white rabbits sequentially, separating the lymphocytes either at room temperature or at 37 degrees c. t cells are lost constantly at room temperature but to a lesser extent at 37 degrees c. the determination of the yield of lymphocytes after ficoll separation gives the best control for the accuracy of the results. if lymphocytes are separated a ...19853889161
isolation of a spirochete from the soft tick, ornithodoros coriaceus: a possible agent of epizootic bovine abortion.a borrelia-like spirochete was detected in all three parasitic stages of ornithodoros coriaceus, the soft tick implicated in the epizoology of epizootic bovine abortion. after the spirochete had been isolated, its distinctness from other north american tick-borne borreliae as well as from spirochaeta aurantia, treponema pallidum, and leptospira interrogans serovar pomona was established on the basis of its morphology, protein components, and inability to infect mice. the spirochete is passed tra ...19853898367
alternative treatment of drug resistant falciparum malaria with particular reference to africa south of the sahara. 19853907533
an acoustic plethysmograph to measure total infant body volume.an acoustic plethysmograph intended to measure the body volume of premature infants has been developed using the principle of the helmholtz resonator, in which the resonance frequency is dependent on the volume of the resonating cavity. a prototype system was built and used to measure the volume of inanimate objects and newborn miniature pigs. results for inanimate objects agree within 1 percent with comparable measurements by water displacement. results of the animal body volume measurements co ...19853908815
cefamandole resistance transfer in bacterial strains from two newborn units.transfer of cefamandole resistance was demonstrated from strains of citrobacter freundii as well as from individual strains of enterobacter cloacae, acinetobacter anitratus and klebsiella pneumoniae isolated from patients in two newborn units. in citrobacter freundii, cefamandole resistance was transferred always with cephalotin resistance as well as with a tem-like beta lactamase (conferring resistance to ampicillin, carbenicillin and azlocillin). citrobacter freundii strains from hospital i we ...19853910723
the sfia11 mutation prevents filamentation in a response to cell wall damage only in a reca+ genetic background.n-alpha-palmitoyl-l-lysyl-l-lysine dihydrochloride ethyl ester (pll) at sublethal doses causes filamentous growth of e. coli strains except sfia mutants, which divide normally in its presence. pll does not elicit the sos responses as judged by lambda prophage induction, an increase of reca protein synthesis or induction of the sfia operon in a sfia::lacz fusion strain. thus, it appears that filamentation caused by pll is not an sos function and might be the result of membrane damage by pll, whic ...19853911030
health effects of nitrogen dioxide and oxidants.the document is an evaluation of the health effects of nitrogen dioxide (no2) and ozone (o3) intended to serve as a basis for establishing swedish air quality standards. the specific effects of nitrogen dioxide and ozone are reviewed on the basis of published studies on animals, tissues and cells, controlled studies on humans, and epidemiologic studies. the focus is on the importance of the gases in relation to sensitive groups, outdoor-indoor exposure relationships, the question of short- or lo ...19853911379
[effects of nitronaphthofurans on the transfer of a plasmid]. 19853915195
[aids to wound healing. from empirical tradition to a scientific approach]. 19853915246
immunology and immunopathology of malignant catarrhal fever. 19853917220
evidence of direct thyroid-stimulating action of thyrotropin-releasing hormone by perifusion of rat thyroid fragments.the possibility that trh has a direct thyroid-stimulating action has never been reported. a number of studies have shown a rise in serum concentrations of thyroid hormone after stimulation with trh, without a rise in tsh secretion. this has led us to test trh with rat thyroid fragment perifusion. significant t4 release was observed for trh concentrations as low as 1.7 x 10(-11) m. a dose-response curve was determined. the response was immediate, reaching a peak after the sixth minute and continu ...19853917904
biologic activity of type i and type ii fusobacterium nucleatum isolates from clinically characterized sites.fusobacterium nucleatum is a microorganism commonly cultured from periodontal disease sites. f. nucleatum isolates (120) were obtained from subgingival plaque samples taken from 27 clinically characterized sites using a selective culture medium. all isolates were verified by morphology, gram-stain reactions, oxygen tolerance and biochemical reactions. a total of eight clinical isolates and two typed strains were used for further evaluation. in this study, there was no relationship found between ...19853925111
inhibition of lymphocyte invasion by sporozoites and the transformation of trophozoite infected lymphocytes in vitro by serum from theileria annulata immune cattle.serum from calves immune to theileria annulata inhibited sporozoite-induced infection and transformation of peripheral blood leucocytes (pbl) into macroschizont infected lymphoblastoid cells in vitro. invasion by sporozoites was suppressed by some sera, especially that obtained after challenge, but in many instances suppression of infection could be attributed only to retardation of development of pbl infected with intracellular trophozoites into proliferating macroschizont infected cells. suppr ...19853925422
subcellular localization of vasopressin-like material in platelets.it has recently been reported that 90% of circulating vasopressin in humans appears to be in platelets. we have confirmed these results and studied the subcellular localization and secretion of vasopressin from human platelets. sucrose density gradient analysis showed highest relative specific activity for vasopressin in the membrane fractions. stirring of platelet-rich plasma with a number of different platelet-aggregating agents including epinephrine, norepinephrine, arachidonic acid, collagen ...19853928782
the establishment of reproducible, complex communities of oral bacteria in the chemostat using defined inocula.nine commonly isolated oral bacterial populations were inoculated into a glucose-limited and a glucose-excess (amino acid-limited) chemostat maintained at a constant ph 7.0 and a mean community generation time of 13.9 h. the bacterial populations were streptococcus mutans atcc 2-27351, strep. sanguis nctc 7865, strep. mitior ef 186, actinomyces viscosus wvu 627, lactobacillus casei ac 413, neisseria sp. a1078, veillonella alkalescens atcc 17745, bacteroides intermedius t 588 and fusobacterium nu ...19853932293
[calcium and magnesium ions in the septum and nucleus accumbens of the rabbit are antagonistic to acupuncture analgesia and morphine analgesia]. 19853939075
[calcium and magnesium ions in the septum and nucleus accumbens of the rabbit are antagonistic to acupuncture analgesia and morphine analgesia]. 19853939075
[riboflavin operon in bacillus subtilis contains additional promoters].using the methods of molecular cloning permitted to show that riboflavin operon of bacillus subtilis contains four promoters. three of them are functionally active in the bacillus subtilis system. the main promoter of the operon with regulatory region was cloned in plasmid ppl603. cells containing the constructed plasmid pgm32 are resistant to chloramphenicol. the level of resistance is regulated by concentration of riboflavin (the effector of operon). the following model of rib-operon has been ...19853939570
trichinella spiralis: newborn larval migration route in rats reexamined.the route by which trichinella spiralis newborn larvae migrate from the small intestine to striated muscle was studied in inbred ao and random-bred sprague-dawley rats. newborn larvae were quantitatively recovered from the thoracic duct lymph, peritoneal cavity, and hepatic portal vein blood during the course of a primary infection with 4000 muscle larvae. the total recovery of newborn larvae assessed in this manner was compared with the number of muscle larvae in control rats receiving the same ...19863943594
relation of animal size to convergence, divergence, and neuronal number in peripheral sympathetic pathways.the enormous range of animal size raises a fundamental problem: how do larger animals maintain adequate control of peripheral structures that are many times more massive and extensive than the homologous structures in smaller animals? to explore this question, we have determined neuronal number, the number of axons that innervate each neuron (convergence) and the number of neurons innervated by each axon (divergence), in a peripheral sympathetic pathway of several mammals (mouse, hamster, rat, g ...19863944617
pseudoephedrine-induced hypertensive emergency: treatment with labetalol.the case of a young man in hypertensive emergency following the ingestion of 840 mg of pseudoephedrine is reported. the hypertension, which peaked at 200/160 mm hg and was associated with severe headache and diaphoresis, responded expeditiously to two initial intravenous doses of labetalol. no rebound was observed following the administration of an additional dose in the intensive care unit, after which an uneventful one-day hospitalization ensued. the toxicity of pseudoephedrine is briefly revi ...19863947442
the influence of prolonged clofibrate treatment on the plasma drug concentration and liver nadp-linked enzymes activity.the clofibric acid (cpib) concentration in the rat blood plasma during 14 days of treatment with clofibrate and after cessation of the treatment, was measured and compared with the changes of some nadp-linked dehydrogenases activity. cpib rapidly accumulates in the plasma during the first two days of clofibrate administration reaching a maximum concentration of about 0.6 mm. then a linear decrease of the drug concentration was observed. the second day after clofibrate treatment had been complete ...19863949145
breast feeding as a determinant of severity in shigellosis. evidence for protection throughout the first three years of life in bangladeshi children.little is known about the effect of breast feeding upon the severity of illness due to specific diarrheal pathogens. using a systematically sampled and evaluated population of children aged less than 3 years, who attended a diarrheal disease hospital in bangladesh, the authors performed a case-control study that assessed whether breast feeding reduces the severity of illness in shigellosis. from 540 children presenting with shigellosis between 1980-1982, they created a group of cases (n = 53) wi ...19863953549
breast feeding as a determinant of severity in shigellosis. evidence for protection throughout the first three years of life in bangladeshi children.little is known about the effect of breast feeding upon the severity of illness due to specific diarrheal pathogens. using a systematically sampled and evaluated population of children aged less than 3 years, who attended a diarrheal disease hospital in bangladesh, the authors performed a case-control study that assessed whether breast feeding reduces the severity of illness in shigellosis. from 540 children presenting with shigellosis between 1980-1982, they created a group of cases (n = 53) wi ...19863953549
pericanalicular location of hepatocyte lysosomes and effects of fasting: a morphometric analysis.lysosomes constitute a small proportion of hepatocyte volume, but they play a key degradative role, particularly during catabolic states such as nutritional deprivation. our preliminary observations in hepatocytes suggested that fasting may alter the distribution of lysosomes, which are thought to congregate about the biliary canaliculus. the current study aimed to provide, in both fed and fasted rats, a quantitative morphometric analysis of lysosomes in the immediate pericanalicular and success ...19863957237
pericanalicular location of hepatocyte lysosomes and effects of fasting: a morphometric analysis.lysosomes constitute a small proportion of hepatocyte volume, but they play a key degradative role, particularly during catabolic states such as nutritional deprivation. our preliminary observations in hepatocytes suggested that fasting may alter the distribution of lysosomes, which are thought to congregate about the biliary canaliculus. the current study aimed to provide, in both fed and fasted rats, a quantitative morphometric analysis of lysosomes in the immediate pericanalicular and success ...19863957237
surface structures (peritrichous fibrils and tufts of fibrils) found on streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera.we screened 36 strains of streptococcus sanguis biotype i and 8 strains of s. sanguis biotype ii for the presence of surface structures and for their ability to coaggregate with actinomyces viscosus, actinomyces naeslundii, and fusobacterium nucleatum. negative staining under an electron microscope revealed detectable surface structures on all s. sanguis strains. the majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. they usually a ...19853965396
interface mechanics and bone growth into porous co-cr-mo alloy implants.the interface mechanics and bone growth into porous co-cr-mo alloy implants were evaluated. three mean pore sizes (155, 235, and 350 microns) were studied for implants fabricated with one, two, and three layers of spherical powder particles and totally porous implants. all implants had a pore volume of 38%-40%. ten implants were inserted transcortically in the femora of six adult mongrel dogs and were allowed to remain in situ for a period of 12 weeks. postretrieval mechanical testing and histol ...19853971631
long-term effects of q-switched ruby laser on monkey anterior chamber angle.in an attempt to clarify whether pulsed lasers might be able to cause permanent fistulas from the anterior chamber to the interior of the canal of schlemm, slightly suprathreshold, low energy, small diameter q-switched ruby laser pulses were applied to the trabecular meshwork of nine eyes of six rhesus monkeys. clinical examinations during the next 2 months disclosed no adverse effect on the cornea, iris, lens, or retina. there was transient mild inflammation in five eyes. intraocular pressure w ...19853972496
long-term effects of q-switched ruby laser on monkey anterior chamber angle.in an attempt to clarify whether pulsed lasers might be able to cause permanent fistulas from the anterior chamber to the interior of the canal of schlemm, slightly suprathreshold, low energy, small diameter q-switched ruby laser pulses were applied to the trabecular meshwork of nine eyes of six rhesus monkeys. clinical examinations during the next 2 months disclosed no adverse effect on the cornea, iris, lens, or retina. there was transient mild inflammation in five eyes. intraocular pressure w ...19853972496
neonatal lupus erythematosus. a report of three cases associated with anti-ro/ssa antibodies.three patients had neonatal lupus erythematosus syndrome. all three infants were girls who presented with characteristic skin lesions but without evidence of congenital heart block. all three mothers were asymptomatic at the time of delivery and have remained so until the present time. all mothers were positive for anti-ro/ssa antibodies, as were the infants prior to 6 months of age. anti-la/ssb antibodies were not present in either the mothers or the infants. hla typing revealed that two of the ...19853977359
descending lumbosacral cord potentials (dlcp) evoked by stimulation of the median nerve.in 22 normal human subjects, descending lumbosacral cord potentials (dlcp) were recorded intrathecally after stimulation of the median nerve at the elbow. the onset of dlcp is very short in latency (mean 12.1 ms) with a prominent sharp early positive peak (mean latency 13.7 ms) followed by a sharp negative peak (mean 17.6 ms). the amplitude of the first part of dlcp varied between 0.6 and 6.7 microv (mean 2.3 microv). the response was recorded most easily when the tip of intrathecal electrode wa ...19853978422
airgun injuries. 19853984193
[reciprocal regulation of growth and differentiation in hepatocytes by cell surface modulator and loss of regulation during carcinogenesis].in primary monolayer cultures of mature rat hepatocytes, cell growth and hepatocyte-specific functions were mediated by a cell surface component (named the cell surface modulator) via cell-cell contact. the modulator activity was heat-labile and trypsin-sensitive. activity was also found in plasma membranes from kidney, brain, lung, and erythrocytes. the modulator was solubilized by 4% octylglucoside plus 4m guanidine hcl from liver membranes. the molecular weight of the modulator was 670kd dete ...19853985639
teratogenic potential of inhaled dichlorobenzenes in rats and rabbits.orthodichlorobenzene (odcb) and paradichlorobenzene (pdcb) were evaluated for teratogenic potential in rats (odcb only) and rabbits. groups of bred rats and inseminated rabbits were exposed to 0, 100, 200, or 400 ppm of odcb; groups of inseminated rabbits were exposed to 0, 100, 300, or 800 ppm of pdcb. animals were exposed for 6 hr/day on days 6 through 15 (rats) or 6 through 18 (rabbits) of gestation. maternal toxicity, as evidenced by a significant decrease in body weight gain, was observed i ...19853987993
serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis.in the present study we sought to determine whether serum antibody was present against microorganisms which predominate in the subgingival flora of young adults with generalized severe periodontitis (sp). subjects with sp were often seropositive for eubacterium brachy, fusobacterium nucleatum e3c22, and peptostreptococcus micros, whereas subjects with juvenile periodontitis (jp) and subjects with healthy periodontium (hp) were not. both sp and jp subjects were more frequently seropositive for ba ...19853988337
microbial ecology of plaque in rats with naturally occurring gingivitis.the microbial ecology of adherent plaque was investigated in relation to the pathological findings of gingivitis in plaque-susceptible rats. plaque developed in the gingiva of the lower incisor in plaque-susceptible rats, but not in plaque-resistant rats, after they were fed a commercial powder diet. with increase in plaque volume, the total counts of bacteria increased 10(9) to 10(11)/g. in the first 3 months, bacteroides species increased and became the predominant population. streptococcus sp ...19853988345
sexual development in beef bulls following zeranol implants.two trials were conducted to study the effect of zeranol implants on growth and sexual development of bull calves. trial 1 compared the effects of implanting with 72 mg of zeranol at 48 d of age (branding), at 215 d of age, or at both times with nonimplanted control bulls. implanting at branding resulted in decreased scrotal circumference, testicle weight and proportion of bulls that could produce an ejaculate at 14 mo of age (p less than .01). implanting at 215 d of age had no effect on any of ...19853988627
relationship of rumen fluid dilution rate to rumen fermentation and dietary characteristics of beef steers.data from seven beef steer trials were compiled and regression analyses used to evaluate relationships among molar proportions of acetate (ac), propionate (pr) and butyrate (bu), total concentration of volatile fatty acids (vfa), rumen ammonia (nh3), rumen ph, rumen fluid dilution rate (fdr), rumen fluid volume (fvol), body weight (wt), dry matter intake (dmi) and dietary concentration and intake of crude protein (cp and cpi), acid detergent fiber (adf and adfi), ash (ash and ashi) and metaboliz ...19853988654
[histochemistry of hyaluronic acid of the bovine vitreous body by electronmicroscopy]. 19853993469
hypothermia produced by tributyl s,s,s-phosphorotrithioate (def).tributyl s,s,s-phosphorotrithioate (def) produces profound hypothermia in rats, mice and guinea pigs by inhibition of thermogenesis. its actions on heat conservation and motor control are, however, minimal. it is effective against both shivering and non-shivering thermogenesis and completely blocks the increase in body temperature evoked by anterior hypothalamic stimulation. a number of other measures indicated that this is unlikely to be due to a lack of peripheral thermogenic capacity: thus pl ...19853994512
acupuncture used as an adjunct in the treatment of a horse with tetanus. 19854004654
cytotoxic effects of culture supernatants of oral bacteria and various organic acids on vero cells.dilute culture filtrates of the three asaccharolytic black-pigmented bacteroides species as well as b. macacae, fusobacterium nucleatum, and animal strains resembling b. gingivalis were cytotoxic for the african green monkey kidney cell line (vero cells). butyric or propionic acid, major metabolic products of these bacteria, seem to be responsible for the characteristic morphological changes observed. among the organic acids studied, only butyric, propionic, and valeric acids exhibited cytotoxic ...19854005713
superinfected actinomycotic abscess of the liver.isolated actinomycotic liver abscess is rare. we report a case with fulminant evolution, which was treated successfully with surgery and penicillin. in human actinomycosis, actinomyces israeli is the main infective agent. an abdominal localisation is uncommon, occurring in less than 25% of cases. isolated liver lesions are extremely rare; their presence might be interpreted as the result of the hematogenous spread of a no longer present intestinal lesion. the evolution of actinomycosis is usuall ...19854019082
activity of a peptidyl prodrug, alafosfalin, against anaerobic bacteria.alafosfalin, an antibacterial phosphonodipeptide requiring peptide transport for activity, was tested for activity against clinical strains of anaerobic bacteria in peptide-free roche sensitivity test medium no. 5 agar. it was active against bacteroides spp., fusobacterium nucleatum, and clostridium perfringens but not against clostridium difficile. alafosfalin activity was antagonized by appropriate peptides. synergy was obtained with other cell wall-active antibiotics.19854026269
assessment of serum antibody patterns and analysis of subgingival microflora of members of a family with a high prevalence of early-onset periodontitis.in a study of members of a large family with a high prevalence of early-onset periodontitis, we sampled the subgingival microflora and characterized 40 isolates from each sample. we surveyed serum samples by enzyme-linked immunosorbent assay for antibodies reacting with any of a panel of 21 periodontal bacteria. the mother and 7 of her 13 children had early-onset periodontitis. bacteroides gingivalis was not detected in the subgingival flora of any affected or unaffected family member, and actin ...19854030102
clinical appearance of orofacial infections of odontogenic origin in relation to microbiological findings.fifty-eight patients with acute orofacial infections of odontogenic origin were classified into two groups with respect to the severity of infection. a total of 174 anaerobic and 22 aerobic bacterial strains were isolated. anaerobic gram-negative rods were isolated more frequently from the patients with severe infections than from the patients with infections judged as mild (p less than 0.05). the occurrence of fusobacterium nucleatum especially appeared to be associated with the severity of the ...19854031041
fatty acid composition and shwartzman activity of lipopolysaccharides from oral bacteria.the composition and the nature of the linkage of fatty acids and the shwartzman activity of lipopolysaccharide (lps) preparations derived from oral gram-negative bacteria including bacteroides gingivalis, bacteroides loesheii, eikenella corrodens, fusobacterium nucleatum, and actinobacillus actinomycetemcomitans were examined. 3-hydroxylated and nonhydroxy fatty acids of various chain lengths were found in all of the lps preparations. all nonhydroxy fatty acids were found to be ester-bound, and ...19854033466
differences in density and distribution of surface glycoconjugates between normal and dystrophic mouse schwann cells detected by statistical analyses of lectin-ferritin binding.cultures of schwann cells and neurons from dorsal root ganglia of normal (c57bl/6j +/+) and dystrophic (c57bl/6j dy2j/dy2j) mice were labeled with wheat germ agglutinin (wga) and ricinus communis agglutinin (rca-i) conjugated to ferritin. statistical methods were used to compare the regional densities and distribution characteristics of lectin binding in these two types of schwann cells, which differ in their capacities to ensheath and myelinate axons in vivo and in cultures. regional variations ...19854041887
aedes (gymnometopa) mediovittatus (diptera: culicidae), a potential maintenance vector of dengue viruses in puerto rico. 19854045943
fluoridation of drinking water: effects on kidney stone formation.the influence of fluoride in drinking water on stone formation was studied in animals and in "in vitro" crystallization experiments. in male wistar rats fluoride inhibits ethylenglycol induced calcification of the kidneys and stone formation. the in vitro results performed in synthetic urine exhibited a dose-dependent delay of crystal growth.19854049603
fluoridation of drinking water: effects on kidney stone formation.the influence of fluoride in drinking water on stone formation was studied in animals and in "in vitro" crystallization experiments. in male wistar rats fluoride inhibits ethylenglycol induced calcification of the kidneys and stone formation. the in vitro results performed in synthetic urine exhibited a dose-dependent delay of crystal growth.19854049603
oxygen transport in isolated cardiac myocytes.a preparation of isolated cardiac myocytes, retaining functional and structural properties of myocytes in the intact heart, has been used to measure intracellular oxygen pressure gradients. these are shallow and do not exceed 2 torr. oxygen does not limit mitochondrial function in the cardiac myocyte until intracellular myoglobin is almost entirely deoxygenated. most of the large oxygen pressure gradient in the working heart must be extracellular.19854062838
oxygen transport in isolated cardiac myocytes.a preparation of isolated cardiac myocytes, retaining functional and structural properties of myocytes in the intact heart, has been used to measure intracellular oxygen pressure gradients. these are shallow and do not exceed 2 torr. oxygen does not limit mitochondrial function in the cardiac myocyte until intracellular myoglobin is almost entirely deoxygenated. most of the large oxygen pressure gradient in the working heart must be extracellular.19854062838
kinetic independence of the subunits of cytosolic glutathione transferase from the rat.the steady-state kinetics of the dimeric glutathione transferases deviate from michaelis-menten kinetics, but have hyperbolic binding isotherms for substrates and products of the enzymic reaction. the possibility of subunit interactions during catalysis as an explanation for the rate behaviour was investigated by use of rat isoenzymes composed of subunits 1, 2, 3 and 4, which have distinct substrate specificities. the kinetic parameter kcat./km was determined with 1-chloro-2,4-dinitrobenzene, 4- ...19854062896
reversible expression of flagella in campylobacter jejuni.campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype.19854066041
evaluation of amniotic fluid in preterm labor with intact membranes.amniotic fluid was obtained from 35 pregnant women in preterm labor with intact membranes. their gestational ages ranged from 24 to 34 weeks. bacteria were detected in only 1 (3%) of the 35 amniotic fluid samples. the anaerobic culture grew bacteroides corrodens and fusobacterium nucleatum. the l/s ratio was greater than 4.0 in six patients (17%), 2.5-4.0 in two (6%) and less than 2.5 in 23 (66%); the quantity of fluid was inadequate for l/s analysis in four (11%). none of the 35 newborns develo ...19854067950
[analgesic effect, tolerance development and dependence potential of d-phenylalanine].in comparison to well-established non-narcotic analgetics, the amino acid d-phenylalanine produces similar, dose-dependent analgesic effects in animal experiments, but acts significantly longer. neither a tolerance of mice to d-phe after administration for 10 d nor a development of drug dependence in rats following treatment for 6 weeks could be regarded.19854070349
oral papillomas in cattle. 19854072491
interaction of dna with tilorone derivatives: viscometric investigations.interaction of dna with tilorone and six of its derivatives was studied in solution. this was done by viscosity measurements on both low and high molecular weight calf thymus dna, in order to interpret the experimental results in terms of changes of dna contour length (intercalation) and of persistence length (stiffening and bending). qualitative and semiquantitative analysis delivered the following results: the carbonyl derivatives of tilorone bind more strongly to dna than tilorone itself. the ...19854084265
[electron microscopic studies of various serratia phages(]. 19704098616
electron microscopy of the in vitro development of mammalian motor end plates. 19714107829
unit and multi-unit electrical activities recorded from the pituitary stalk of the sheep. 19724110393
[more extensive differentiation of the dynamics of pathomorphic immunity phenomena in experimental allergic encephalomyelitis]. 19724113339
[human adiaspiromycosis due to fungus emmonsia crescens sp. n. emmons et jellison 1960]. 19714115100
defect of cell-mediated immunity in patients with iron-deficiency anaemia. 19724117379
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