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primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen.the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ...19863028680
primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen.the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ...19863028680
oligosaccharides obtained by partial hydrolysis of lipopolysaccharide from fusobacterium nucleatum f1. 19862937904
[abnormal location of ligula intestinalis l. in scardinius erythrophthalmus l. from lake trasimeno]. 19862944464
the effect of phenytoin on glutamate and gaba transport.phenytoin was observed to inhibit competitively the sodium dependent high affinity synaptosomal transport of both glutamate (glu) and gamma-aminobutyric acid (gaba) with ki values of 66 +/- 10 and 185 +/- 65 microm, respectively. this contrasted with a previous report that the uptakes of glu and gaba were enhanced by phenytoin. the degree of inhibition is dependent on the concentrations of the competing drug and substrate present. taking the therapeutic levels of phenytoin and the overall brain ...19862946971
an ultrastructural study of nerve cell bodies in the carotid body and common carotid artery of the domestic fowl. 19862948419
[morphologic studies following coronary thrombolysis and ptca].compared with those of other species human coronary arteries show a particularly marked diffuse thickening of the intima. superimposed on this diffuse thickening local sclerotic changes develop. in early stages they contain plenty of lipids which are mainly stored in modified smooth muscle cells, partly also in cells of monocytic origin. normally ruptures of arteriosclerotic plaques are responsible for thrombotic occlusions in extensive transmural myocardial infarctions. they are caused by ruptu ...19862950674
[malaria prevention. chemotherapy and immune prophylaxis. 3. immune response of infected organism. perspectives of malaria prevention]. 19862423092
humoral antibody response against bacteroides gingivalis-specific antigen recognized by monoclonal antibody in adult periodontal patients.an antigen recognized by monoclonal antibody specific for bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. the purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and silver staining, and the pattern of sds-page agreed with that of immunoblotting. the molecule exhibited an apparent molecular weight of about 62,000 by sds-page. the antigen was sensitive ...19862426200
[activity of cefotetan against anaerobic bacteria].we determined the susceptibility to antibiotics of 64 strains of obligate anaerobes. tested bacterial strains were as follows: 28 bacteroides fragilis, 22 fusobacterium nucleatum, 10 clostridium perfringens, and 4 bacteroides bivius. tested antibiotics were cefotetan, cefoxitin and piperacillin. cefotaxime was tested against 20 bacteroides fragilis strains. bacteroides fragilis, which is the most resistant species, was susceptible to cefotetan, cefoxitin and piperacillin, with comparable mics fo ...19862877431
homocysteic acid, an endogenous agonist of nmda-receptor: release, neuroactivity and localization. 19862878563
ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.deoxyribonucleic acid from 41 different bacterial species (including gram-negative and gram-positive species) were cleaved by different restriction endonucleases, electrophoresed in agarose and transferred to nylon filters. the dna fragments carrying rrna genes (rdna) were localized by hybridization with a 32p-labelled escherichia coli 16 + 23s rrna probe. a pattern of hybridized fragments was obtained for each dna tested. within a bacterial species (defined as a dna hybridization group), one or ...19862446639
trichinella spiralis: newborn larval migration route in rats reexamined.the route by which trichinella spiralis newborn larvae migrate from the small intestine to striated muscle was studied in inbred ao and random-bred sprague-dawley rats. newborn larvae were quantitatively recovered from the thoracic duct lymph, peritoneal cavity, and hepatic portal vein blood during the course of a primary infection with 4000 muscle larvae. the total recovery of newborn larvae assessed in this manner was compared with the number of muscle larvae in control rats receiving the same ...19863943594
relation of animal size to convergence, divergence, and neuronal number in peripheral sympathetic pathways.the enormous range of animal size raises a fundamental problem: how do larger animals maintain adequate control of peripheral structures that are many times more massive and extensive than the homologous structures in smaller animals? to explore this question, we have determined neuronal number, the number of axons that innervate each neuron (convergence) and the number of neurons innervated by each axon (divergence), in a peripheral sympathetic pathway of several mammals (mouse, hamster, rat, g ...19863944617
pseudoephedrine-induced hypertensive emergency: treatment with labetalol.the case of a young man in hypertensive emergency following the ingestion of 840 mg of pseudoephedrine is reported. the hypertension, which peaked at 200/160 mm hg and was associated with severe headache and diaphoresis, responded expeditiously to two initial intravenous doses of labetalol. no rebound was observed following the administration of an additional dose in the intensive care unit, after which an uneventful one-day hospitalization ensued. the toxicity of pseudoephedrine is briefly revi ...19863947442
the influence of prolonged clofibrate treatment on the plasma drug concentration and liver nadp-linked enzymes activity.the clofibric acid (cpib) concentration in the rat blood plasma during 14 days of treatment with clofibrate and after cessation of the treatment, was measured and compared with the changes of some nadp-linked dehydrogenases activity. cpib rapidly accumulates in the plasma during the first two days of clofibrate administration reaching a maximum concentration of about 0.6 mm. then a linear decrease of the drug concentration was observed. the second day after clofibrate treatment had been complete ...19863949145
breast feeding as a determinant of severity in shigellosis. evidence for protection throughout the first three years of life in bangladeshi children.little is known about the effect of breast feeding upon the severity of illness due to specific diarrheal pathogens. using a systematically sampled and evaluated population of children aged less than 3 years, who attended a diarrheal disease hospital in bangladesh, the authors performed a case-control study that assessed whether breast feeding reduces the severity of illness in shigellosis. from 540 children presenting with shigellosis between 1980-1982, they created a group of cases (n = 53) wi ...19863953549
breast feeding as a determinant of severity in shigellosis. evidence for protection throughout the first three years of life in bangladeshi children.little is known about the effect of breast feeding upon the severity of illness due to specific diarrheal pathogens. using a systematically sampled and evaluated population of children aged less than 3 years, who attended a diarrheal disease hospital in bangladesh, the authors performed a case-control study that assessed whether breast feeding reduces the severity of illness in shigellosis. from 540 children presenting with shigellosis between 1980-1982, they created a group of cases (n = 53) wi ...19863953549
pericanalicular location of hepatocyte lysosomes and effects of fasting: a morphometric analysis.lysosomes constitute a small proportion of hepatocyte volume, but they play a key degradative role, particularly during catabolic states such as nutritional deprivation. our preliminary observations in hepatocytes suggested that fasting may alter the distribution of lysosomes, which are thought to congregate about the biliary canaliculus. the current study aimed to provide, in both fed and fasted rats, a quantitative morphometric analysis of lysosomes in the immediate pericanalicular and success ...19863957237
pericanalicular location of hepatocyte lysosomes and effects of fasting: a morphometric analysis.lysosomes constitute a small proportion of hepatocyte volume, but they play a key degradative role, particularly during catabolic states such as nutritional deprivation. our preliminary observations in hepatocytes suggested that fasting may alter the distribution of lysosomes, which are thought to congregate about the biliary canaliculus. the current study aimed to provide, in both fed and fasted rats, a quantitative morphometric analysis of lysosomes in the immediate pericanalicular and success ...19863957237
head-space gas-liquid chromatographic analysis for presumptive identification of bacteria in blood cultures.a total of 445 strains of common aerobic and anaerobic bacteria were analyzed for their volatile microbial metabolites by head-space gas-liquid chromatography. the bacteria were incubated for 24 or 48 h in blood-containing trypticase soy or lombard-dowell glucose broth before being analyzed by automatic head-space injection. from the chromatographic pattern obtained, the majority of klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, escherichia coli, pseudomonas aeruginosa, morgan ...19863816354
[fertilization of human oocytes in vitro and embryo transplantation]. 19863817215
distribution of alpha-amylase activity in selected broiler tissues.in an examination of broiler alpha-amylase, significant variation in the serum enzyme activity level was noted, adult levels were lower than those of young chicks. analysis of alpha-amylase activity in various body fluids and tissues of 11-day and 7-week-old broilers indicated that the liver cannot be considered a source of alpha-amylase, although there was activity in both liver tissue and bile of 10 units/g wet weight and 35 units/100 ml, respectively. fluid from the oral cavity had low levels ...19863486408
mucosal induction of systemic t cell tolerance by fusobacterium nucleatum.the present study was undertaken to determine if mucosal presentation of the periodontopathic bacterium fusobacterium nucleatum could induce systemic tolerance. two separate protocols of mucosal priming were carried out. in the first, mice were gastrically intubated on 2 consecutive days; this was repeated 5 days later. in the second protocol, mice were similarly primed but received another priming dose after a further 7 days. positive control mice were similarly primed with sheep red blood cell ...19863488389
extensive proliferation of mature connective-tissue type mast cells in vitro.there are two phenotypically distinct subpopulations of mast cells in rodents: connective tissue-type mast cells (ctmc) and mucosal mast cells (mmc). these populations differ in their location, cell size, staining characteristics, ultrastructure, mediator content and t-cell dependency. several investigators recently reported a further subclass of mast cells which arise when normal mouse haematopoietic cells are cultured with interleukin-3 (il-3); il-3 is an activity similar or identical to mast- ...19863491321
dna reactivity and in vitro cytotoxicity of the novel antitumor agent 1,5,2,4-dioxadithiepane-2,2,4,4-tetraoxide (nsc-348948) in human embryo cells.1,5,2,4-dioxadithiepane-2,2,4,4-tetraoxide (cyclic-soso) is structurally a novel synthetic compound but may functionally act as an alkylating agent. the effects of cyclic-soso on dna were studied in imr-90 and va-13 human embryo cells by means of dna alkaline elution analysis. in contrast to a number of bifunctional alkylating agents, cyclic-soso produced no dna-dna interstrand cross-links in either cell line, even at concentration which produced a greater than 3 log cell kill. at equimolar conc ...19863509975
a borrelia-specific monoclonal antibody binds to a flagellar epitope.in immunofluorescence assays monoclonal antibody h9724 recognized eight species of the spirochetal genus borrelia but not representatives of the genera treponema, leptospira, and spirochaeta. we examined the reactivity of h9724 against subcellular components of borrelia hermsii, an agent of relapsing fever, and b. burgdorferi, the cause of lyme disease. h9724 bound to isolated periplasmic flagella of the two borreliae. in western blots the antibody reacted with the predominant protein in flagell ...19863516878
[nosocomial infections caused by klebsiella pneumoniae in a neonatal unit and a unit for low-birth-weight infants]. 19863518962
attachment level changes in destructive periodontal diseases.the present communication attempts to summarize some of the features of attachment loss which are of interest to the clinician and the statistician analyzing data from clinical trials. these include the measurements employed to detect changes in attachment level, the nature of the destructive disease process and the effects of therapy on the attachment level measurements. although there are several difficulties associated with the attachment level measurement, at the present time it appears to b ...19863522651
[monoclonal antibodies to the antigens of human hela tumor cells].a panel of 6 hybridomas "xejima" producing monoclonal antibodies specific to hela cells is prepared. monoclonal antibodies do not bind to antigens of human diploid fibroblasts, human continuous b- and t-lymphocytes and animal cell lines. the specificity of monoclonal antibodies to cellular antigens of 5 hela-like cell lines and 6 human tumour cells lines, not contaminated with hela cells, is determined. antibody containing ascitic fluid and culture media of hybridomas xejima-3, -12, -13, and -22 ...19863526672
[monoclonal antibodies to the antigens of human hela tumor cells].a panel of 6 hybridomas "xejima" producing monoclonal antibodies specific to hela cells is prepared. monoclonal antibodies do not bind to antigens of human diploid fibroblasts, human continuous b- and t-lymphocytes and animal cell lines. the specificity of monoclonal antibodies to cellular antigens of 5 hela-like cell lines and 6 human tumour cells lines, not contaminated with hela cells, is determined. antibody containing ascitic fluid and culture media of hybridomas xejima-3, -12, -13, and -22 ...19863526672
[sensitivity of anaerobes to 8 antibiotics].we studied the susceptibility of 282 obligate anaerobes to 8 antibiotics. the strains were isolated from clinical specimens taken during 1983 and 1984. minimal inhibitory concentrations (mics) were determined using a microplate method for 245 strains and agar dilution for 33 bacteroides asaccharolyticus strains. tested strains were as follows: 85 bacteroides fragilis, 71 clostridium, 19 fusobacterium nucleatum, 35 peptostreptococcus, 39 bacteroides oralis and bacteroides bivius and 33 bacteroide ...19863534760
[origin, course and recession of an infectious disease, melioidosis, in temperate countries]. 19863535711
induction of bacterial mutations by aminopyrazoles, compounds which cause mammary cancer in rats.an aminopyrazole pd 71627 (5-amino-1,3-dimethyl-1h-pyrazol-4-yl) (2-fluorophenyl)methanone, and two amide derivatives, pd 108298, n-[4-(2-fluorobenzoyl)-1,3-dimethyl-1h-pyrazol-5-yl]-2- ([3-(2-methyl-1- piperidinyl)-propyl]amino) acetamide-(z)-2-butanedioate (1:2), and pd 109394, 2-(diethylamino)-n-[4-(2- fluorobenzoyl)-1,3-dimethyl-1h-pyrazol-5-yl]acetamide hydrochloride, proposed neuroleptic drugs, were found to elicit mammary adenocarcinomas in male rats after 13 weeks of treatment. these com ...19863536141
immunohistochemical study of the distribution of vasotocin reacting neurons in avian diencephalon.vasotocin (vt)- and neurophysin (nph)-containing neurons were immunocytochemically demonstrated in the diencephalon of the japanese quail (coturnix coturnix japonica), domestic fowl (gallus gallus), and pekin duck (anas platyrhynchos). in these three avian species, the immunoreactive cells are similarly distributed into three different diencephalic regions: lateral (l), periventricular (p), and dorsal diencephalic (dd). a different number of cell clusters can be easily identified in each region ...19863540108
immunohistochemical study of the distribution of vasotocin reacting neurons in avian diencephalon.vasotocin (vt)- and neurophysin (nph)-containing neurons were immunocytochemically demonstrated in the diencephalon of the japanese quail (coturnix coturnix japonica), domestic fowl (gallus gallus), and pekin duck (anas platyrhynchos). in these three avian species, the immunoreactive cells are similarly distributed into three different diencephalic regions: lateral (l), periventricular (p), and dorsal diencephalic (dd). a different number of cell clusters can be easily identified in each region ...19863540108
mechanisms of beta-lactam resistance in anaerobic bacteria.the known mechanisms of beta-lactam resistance in anaerobic bacteria involve production of beta-lactamases, alteration of penicillin-binding proteins, and blocking of the penetration of beta-lactams through the outer membranes. the most important factor in beta-lactam resistance is the production of beta-lactamase. beta-lactamases in various bacteroides, fusobacterium, and clostridium species have been described. beta-lactam resistance in bacteroides fragilis is most commonly mediated by the pro ...19863541135
isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase.the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ...19863542024
x-ray scattering and diffraction by wet gels of aa amyloid fibrils.systemic amyloidosis is characterized by the extracellular accumulation of protein fibrils with typical ultrastructural morphology. the persistence in vivo of amyloid fibrils, which is responsible for their serious clinical effects, has been thought to reflect the particular, specific conformation of peptide chains constituting the fibrils. on the basis of earlier structural studies this conformation is generally considered to be almost exclusively anti-parallel beta-sheets. we have re-examined ...19863561252
dna probes for identification of clinically important bacteroides species.conventional procedures for identifying gram-negative anaerobes such as bacteroides spp. are cumbersome and time-consuming. a simpler approach would be to use dna probes to identify these organisms. since many different species of gram-negative anaerobes are isolated from clinical specimens, the most useful dna probes would be probes that identify a few clinically significant groups of anaerobes. to obtain such probes, we cloned hindiii-digested chromosomal dna from various bacteroides species a ...19863700617
[lithotripsy by ultrashort pulsation. experimental study in renal lithiasis in the dog].a lithotriptor delivering ultrashort shockwaves and designed for use under ultrasonographic guidance has recently been developed. immersion in water is unnecessary. the device was experimented in renal lithiasis in dogs. twenty-two animals were treated. in seven, who served as controls, the safety of shots delivered in particular to the lungs, liver, bones and kidneys was demonstrated. furthermore, no cardiac rhythm disorders were recorded. a human stone was implanted in 15 animals. the stone wa ...19863707073
local tolerance evaluation of intramuscular drug preparations. 19863709769
sensitization with fusobacterium nucleatum targets antibody-dependent cellular cytotoxicity to mammalian cells.incubation of mammalian tumor cells with either soluble of insoluble fractions (10 to 100 micrograms/ml) of fusobacterium nucleatum sensitizes them to the destructive activity of antibody-dependent cellular cytotoxicity (adcc) effector cells in the presence of anti-f. nucleatum antisera. all three types of adcc effector cells are capable of destroying f. nucleatum-sensitized target cells with varying degrees of effectiveness (lymphocytes much greater than monocytes greater than neutrophils). hyp ...19863710577
bvd virus infection and embryo transfer. 19863716157
absence of role of endothelium in the response of isolated porcine coronary arteries to acetylcholine.isolated precontracted arteries of various vascular beds relax in response to acetylcholine only if the endothelium is present. one explanation for this is that this drug stimulates the endothelial cells to release a vasodilator substance that in turn relaxes the underlying smooth muscle. to determine whether this mechanism is concerned also in the acetylcholine contraction of isolated porcine coronary arteries transverse strips of the extramural part of the left circumflex artery were used for ...19863719611
dietary protein, enhancement of n-nitrosomethylurea-induced mammary carcinogenesis, and their effect on hormone regulation in rats.the effect of supplemental dietary protein (casein) fed with high fat diets was investigated using the n-nitrosomethylurea-induced mammary tumor model. isocaloric diets containing casein and corn oil at 19 and 15% (normal protein-high fat) or 33 and 15% (high protein-high fat) were fed ad libitum to sprague-dawley mother rats. female offspring continued on the diet. food consumption and growth curves were similar over the entire growth period. n-nitrosomethylurea (50 mg/kg body weight) or saline ...19863731097
3,3',5'-triiodothyronine inhibits iodothyronine-5'-deiodinating activity induced by 3,5,3'-triiodothyronine at equimolar concentrations in cultured fetal mouse liver.to investigate the rt3 effect on iodothyronine-5'-deiodinating activity (i-5'-da) in cultured fetal mouse liver, liver on the 19th day of gestation, in which little or no i-5'-da was detected and, therefore, rt3 was very stable, was cultured in medium containing thyroid hormone-depleted fetal calf serum supplemented with cortisol, insulin, and various concentrations of iodothyronines. after 4-15 days of culture, i-5'-da in the homogenate was assessed by the amount of iodide released from outer r ...19863732155
demonstration of 20k growth hormone in human plasma by gel electrophoretic-immunostaining-autoradiographic assay (geisaa).a variant of human growth hormone (hgh), in which 15 amino acids are missing (commonly referred to as 20k-hgh in contrast to the traditional form which is referred to as 22k-hgh), is known to exist in human pituitary glands. however, lack of a method to measure it in blood has hindered investigations of its physiopathology. we have applied a newly-developed technique called geisaa for its detection in small volumes of human plasma. the method is based upon the lower mr of the variant and its abi ...19863732988
the ionization behavior of bile acids in different aqueous environments.the ionization behavior of cholic acid, deoxycholic acid, and chenodeoxycholic acid in a variety of physiologically important molecular environments was studied using 13c nmr spectroscopy. the apparent pka of the carboxyl group was determined from titration curves obtained from the dependence of the carboxyl carbon chemical shift on ph. using 90% 13c isotopic substitution of the carboxyl carbon, a complete titration curve was obtained for cholate at a concentration below its critical micelle con ...19863734630
nitrogen conversion factors for the proteinaceous content of gums permitted as food additives.nitrogen conversion factors for gum arabic (acacia senegal (l.) willd.), gum tragacanth (asiatic astragalus spp.), gum karaya (sterculia spp.), guar gum (cyamopsis spp.), locust bean (carob) gum (ceratonia spp.), tara gum (caesalpinia spp.), and xanthan gum (xanthomonas campestris) have been calculated from data for the amino acid compositions of their proteinaceous components. the factors derived differ from the arbitrary values (5.7 or 6.25) at present specified by international regulatory aut ...19863743832
concentration-dependence of halothane metabolism in rabbits. 19863744878
biochemistry of maternal milk in early lactation.the analysis of more than 550 human milk samples showed that triglycerides (accounting for 96-99 per cent of the total lipids) increased from 2 to 3.5 g/100 ml mainly during the first week postpartum and remained constant thereafter. in contrast, both cholesterol and vitamin e concentrations decreased from 35 to 20 mg/100 ml and from 1.7 to 0.30 mg/100 ml, respectively. the phospholipids remained constant (40 mg/100 ml). the fatty acid composition of total lipids showed remarkable changes. mid-c ...19863744886
diplostomum spathaceum (rud. 1819): effects of physical factors on the infection of rainbow trout (salmo gairdneri) by cercariae.the relationship between infection rates of rainbow trout (salmo gairdneri) by diplostomum spathaceum and cercarial concentration, water flow rate and temperature were investigated by means of controlled infections within a flume. a linear relationship was obtained between cercarial concentration and mean abundance of metacercariae/fish. a biphasic relationship occurred between flow rate and abundance of metacercariae. within the confines of the flume, it was possible to control the infection ra ...19863748615
[procedure for the biological testing of substances in the guinea pig subcutaneous test. i. the results of an improved semiquantitative assessment of the histological criteria].different materials were implanted into the subcutaneous connective tissue of guinea pigs to evaluate biotolerance by means of the tissue response. the investigations were carried out with reference materials and newly developed implant materials in short-term studies and in case control studies. the semiquantitative valuation standards lead mostly only to informing statements in the sense of a compatibility degree. alternative assertions are possible when comparing very different materials.19863751202
spinal and supraspinal mechanisms for morphine-pentobarbital antinociceptive interaction in relation to cardiac acceleration response in rats.in rat experiments, morphine-pentobarbital antinociceptive interaction affecting cardiac acceleration in response to somatic noxious stimulation was analyzed with the use of intrathecal and intracerebroventricular injections of morphine. cardiac acceleration response was induced by tail compression, and heart rate was monitored by electrocardiogram. pentobarbital, in a subanesthetic intravenous dose, antagonized the antinociceptive effect of morphine in relation to cardiac acceleration response ...19863754702
epidemiologic studies on possible health effects of intake of pyrolyzates of foods, with reference to mortality among japanese seventh-day adventists.to elucidate the effect of intake of mutagenic and/or carcinogenic pyrolysis products of proteins and amino acids on carcinogenesis in man, we have undertaken two epidemiologic cohort studies: one concerning the possible association of broiled fish consumption with cancer and the other concerning the cancer mortality among japanese seventh-day adventists. the main findings of these studies are described.19863757950
[effects of ambroxol on pulmonary surfactant--analysis of the fatty acid composition of phosphatidylcholine in the sputum and normal respiratory tract fluid in rabbits].the mechanism of action of secretagogic expectorants (ex., ambroxol) has not been clarified. recently, attention has been directed to the relationship of their action to pulmonary surfactants. in the present study, the fatty acid (fa) composition of phosphatidylcholine (pc), which is the main physiological surfactant, was investigated using sputum, respiratory tract fluid, mucin-like substance, lung washings and lung tissue of rabbits. effects of ambroxol (20 mg/kg, i.s.) on several parameters s ...19863758876
progesterone receptor synthesis and degradation in mcf-7 human breast cancer cells as studied by dense amino acid incorporation. evidence for a non-hormone binding receptor precursor.we have used the technique of density labeling of proteins by biosynthetic incorporation of 2h, 13c, 15n (dense) amino acids to study the synthesis and degradation rates of the progesterone receptor in mcf-7 human breast cancer cells. in cells grown in the absence of progestin, sucrose gradient shift analyses reveal that it takes 17 h for the normal density progesterone receptor levels to be reduced to half the initial value, whereas in the presence of 10 nm of the synthetic progestin [3h]r5020, ...19863759961
outer membrane proteins of fusobacterium nucleatum fev1.outer membrane enriched material from six strains of fusobacterium nucleatum was analysed by sds-page. the protein profiles of all the strains were dominated by proteins with molecular masses of about 40 kda, and a very high degree of homology in relation to apparent molecular masses was observed. in all strains except fev1, one of the most dominant proteins exhibited heat modifiable properties, having an apparent molecular mass of about 38 kda and 42 kda when heated in sds at 50 and 100 degrees ...19863760817
clinical, pathogenetic, and laboratory features of capnocytophaga infections.granulocytopenia and oral mucosal defects have been reported to be important predisposing factors to recently recognized cases of capnocytophaga septicemia. the authors call attention to an apparent preponderance of these cases in the pediatric age group and emphasize laboratory features which they have found helpful in the diagnosis of capnocytophaga infections. thirteen patients with capnocytophaga infections were seen during a seven-year period. seven of these patients had capnocytophaga bact ...19863766465
action of surfactants on porcine heart malate dehydrogenase isoenzymes and a simple method for the differential assay of these isoenzymes.the cationic surfactant, cetyl (hexadecyl) trimethylammonium bromide (ctab), completely inactivates porcine heart cytoplasmic malate dehydrogenase (l-malate:nad+ oxidoreductase, ec 1.1.1.37) at concentrations (of surfactant) which do not affect the activity of the mitochondrial isoenzyme. these concentrations are close to, or higher than, the critical micelle concentration of ctab. an increase in the ionic strength of the medium significantly retards the ctab-induced inactivation of the cytoplas ...19863768404
acute hypokalemic effect of gossypol.clinical trials had demonstrated that chronic ingestion of gossypol induced infertility in males. hypokalemia of various severities were reported in volunteers taking gossypol. the purpose of this study is to investigate the effects of acute gossypol infusion into alkalotic rats. plasma potassium concentration decreased from 4.08 to 2.87 mm after gossypol infusion with minimal change in urinary potassium excretion. the hypokalemic effect of gossypol was also observed in nephretomized rats. some ...19863768796
acute yersinia infections in man increase intestinal permeability for low-molecular weight polyethylene glycols (peg 400).the passive intestinal permeability of patients with acute diarrhoea was investigated with a liquid meal of oral rehydration fluid containing differently sized, low-molecular weight polyethylene glycols (peg 400). the recovery of test molecules was measured in 6 h specimens of urine. the subjects suffered from acute infections caused by yersinia bacteria accompanied by joint pains. they were investigated during the infection and at least 3-4 weeks later when they had recovered clinically. it was ...19863775268
acute yersinia infections in man increase intestinal permeability for low-molecular weight polyethylene glycols (peg 400).the passive intestinal permeability of patients with acute diarrhoea was investigated with a liquid meal of oral rehydration fluid containing differently sized, low-molecular weight polyethylene glycols (peg 400). the recovery of test molecules was measured in 6 h specimens of urine. the subjects suffered from acute infections caused by yersinia bacteria accompanied by joint pains. they were investigated during the infection and at least 3-4 weeks later when they had recovered clinically. it was ...19863775268
[changes in the structure of pulmonary blood vessels and bronchi in progressive bronchial stenosis]. 19863781307
[the delta virus: the cuckoo's egg in the nest of hepatitis b virus]. 19863785445
sem studies of acellular glomerular basement membrane in human diabetic glomerulopathy.previous transmission electron microscopic studies have demonstrated glomerular basement membrane (gbm) thickening and mesangial matrix (mm) expansion in chronic stages of diabetes. it is difficult, however, to achieve an appreciation of gbm surface features and distribution of mm in planar views. in the current study, autopsy human renal cortical tissue from patients with end-stage diabetic nephropathy were minced and rendered acellular with detergents prior to fixation, cryofracture, and prepa ...19863789417
microenvironment of the enzyme-bound nadh is different in lobster and pig muscle glyceraldehyde-3-phosphate dehydrogenase microcrystals.two possible consequences of crystal lattice formation were studied with glyceraldehyde-3-phosphate dehydrogenases isolated from lobster (palinurus vulgaris) and pig muscle: changes in the microenvironment of the nadh-binding site as detected by fluorescence polarization, and differences in the maximal activities of the microcrystalline enzymes as compared to those in solution. in solution practically no difference was found between the polarization values of the enzyme-nadh and the catalytic in ...19863789735
survival of erythroid burst-forming units and erythroid colony-forming units in canine bone marrow cells exposed in vitro to 1 mev neutron radiation or x rays.conditioned media (cm) from allogeneic stimulated cultures of light density cells (less than 1.08 g/cm3) from the peripheral blood of normal dogs were used to stimulate the growth of erythroid burst-forming units (bfu-e) in bone marrow from normal dogs. maximum numbers of bfu-e were obtained when 5% (vol/vol) 3 x cm and 2 u/ml erythropoietin were added to plasma clot cultures of bone marrow cells. in addition, the radiation sensitivity (d0 value) was determined for cfu-e and for bfu-e in bone ma ...19863797639
address given at a diploma ceremony at boksburg-benoni hospital on 20 june 1979. patient-response to hospitalisation. 1986260906
inhibition of cytokinesis in friend leukemia cells by membrane mobility agents.treatment of a line of friend leukemia cells with a dispersion of the membrane mobility agent, a2c, yields cells that undergo successive nuclear divisions without cytokinesis, resulting eventually in cells with as many as 30 nuclei. neither the dna replication rate of the cells nor the generation time is different after treatment; in addition, the multiple nuclei divide synchronously, and the chromosome number corresponds to the number of nuclei in the cell. inhibition of cytokinesis is not obse ...1986268639
the ohio nursing situation: a report commissioned by the ona board of directors. 1987259967
[children's conditions in the hospital. children's matters are matter of our essential future resources]. 1987260409
didemnin b--an immunosuppressive cyclic peptide that stimulates murine hemagglutinating antibody responses and induces leukocytosis in vivo.didemnin b (db) is a cyclic peptide with potent immunosuppressive activity in vitro and in the murine graft-versus-host-reaction (gvhr), the only measure of in vivo immunity tested in our prior studies. because continued production of mature leukocytes by bone marrow and an intact antibody response are crucial to defense against infection in immunosuppressed patients, we have evaluated the effects of db on these processes as well. anti-sheep red blood cell (srbc) hemagglutinating antibody (hab) ...19873798555
[neuroblastoma; biology and prognostic factors].recently accumulated data on the biology of the tumors of neuroblastoma group are reviewed. histopathological classification system developed by shimada et al, elevated levels of serum neuron-specific enolase and ferritin, and gene abnormalities including partial deletion of the short arm of chromosome #1 and n-myc gene amplification are discussed as well as their prognostic significance. also, the current activities of ccsg (childrens cancer study group) neuroblastoma studies are briefly report ...19873800408
ehlers-danlos syndrome. a variant characterized by the deficiency of pro alpha 2 chain of type i procollagen.the clinical findings and biochemical studies are described for a 30-year-old man with ehlers-danlos syndrome. type analysis of collagen produced by cultured fibroblasts revealed the lack of a detectable pro alpha 2 chain of type i procollagen. the intracellular degradation rate of newly synthesized collagen was higher than that of normal cells, resulting in the reduction of net collagen production. clinical manifestations characterized by cardiovascular abnormalities due to decreased collagen d ...19873800425
[action of a weak alternating magnetic field on oxygen transport in the body in nonspecific inflammatory lung diseases]. 19873562070
[action of a weak alternating magnetic field on oxygen transport in the body in nonspecific inflammatory lung diseases]. 19873562070
2,3,7,8-tetrachlorodibenzo-p-dioxin-induced lipid peroxidation in hepatic and extrahepatic tissues of male and female rats. 19873566341
2,3,7,8-tetrachlorodibenzo-p-dioxin-induced lipid peroxidation in hepatic and extrahepatic tissues of male and female rats. 19873566341
effect of hypothermia on intestinal adaptation and carcinogenesis in the rat.postoperative hyperplasia enhances experimental intestinal carcinogenesis, but the effects of nonsurgical adaptation are uncertain. the tropic and tumour-promoting potentials of moderate hypothermia were tested in two groups of male sprague-dawley rats housed at 10 degrees c for 30 weeks. one group (n = 10) received a 6-week course of azoxymethane (total dose 90 mg kg-1). the second group (n = 7) acted as hypothermic controls. another 2 groups maintained at 22 degrees c received azoxymethane (n ...19873567060
abnormal calcium handling by platelets in thrombotic disorders.this study presents a quantitative comparison of the free cytoplasmic calcium concentration ([ca2+]cyt) and the free concentration in the lumen of the dense tubules of the human platelet. the former was measured by the fluorescence of the high affinity indicator quin2 and latter by the fluorescence of chlorotetracycline (ctc). the ctc technique monitors calcium-ctc complex accumulation in the lumen of dense tubules and mitochondria when washed platelets were incubated in 2 mm ca2+. resting cytop ...19873581444
bacteria-phagocyte interactions: fusobacterium-induced secretion of a neutrophil self-regulatory factor.direct interaction between fusobacterium nucleatum and human neutrophils resulted in the secretion of a neutrophil self-regulatory factor(s). the secretion of this factor was bacteria specific, and depended on the integrity of the bacteria cell surface. factor secretion occurred within 15 min of bacteria--neutrophil interaction. pre-treatment of neutrophils with cytochalasin b but not sodium fluoride inhibited factor secretion. the factor was sensitive to trypsin and heat treatment. ultrafiltrat ...19873583320
direct modulation of human neutrophil adherence by coaggregating periodontopathic bacteria.direct interaction between fusobacterium nucleatum and pmns produced consistent enhancement of pmn adherence. in contrast, consistent suppression of pmn adherence was observed after direct interactions between pmns and bacteroides gingivalis or actinomyces viscosus. the modulatory effects of these periodontopathic bacteria on pmn function could be seen as early as 2 min after interaction. the effects were abrogated by alterations of bacterial structural integrity with heat, formalin or ultrasoni ...19873583410
[proof of illegitimacy and identifying the true father after 70 years].a man born during the first world war doubted his legitimacy and believed his true father to be his mother's (since deceased) cohabitant. his and his sister's findings in less than or equal to 24 blood group systems produced a probability of sibship of w = 33%. the probability of half-sibship between the proband and two of the cohabitant's legitimate children was w = 82%, between him and two other children w = 70%, and between him and yet another two children w = 73%. these values raised doubts ...19873591019
renal handling of thyroxine, 3,5,3'- and 3,3',5'-triiodothyronine, 3,3'- and 3',5'-diiodothyronine in man.the 24-h urinary excretion and renal clearance of thyroxine (t4), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (rt3), 3,3'-diiodothyronine (3,3'-t2), and 3',5'-diiodothyronine (3',5'-t2) were measured in 17 healthy subjects. the median urinary excretion was (pmol/24h) t4: 1242, t3: 828, rt3: 12.9, 3,3'-t2: 331, and 3',5'-t2: 5.8. the corresponding renal clearances were in median (ml/min) t4: 31, t3: 133, rt3: 15, 3,3'-t2: 683, and 3',5'-t2: 4.5. the clearances differed mutually (p less ...19873591221
renal handling of thyroxine, 3,5,3'- and 3,3',5'-triiodothyronine, 3,3'- and 3',5'-diiodothyronine in man.the 24-h urinary excretion and renal clearance of thyroxine (t4), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (rt3), 3,3'-diiodothyronine (3,3'-t2), and 3',5'-diiodothyronine (3',5'-t2) were measured in 17 healthy subjects. the median urinary excretion was (pmol/24h) t4: 1242, t3: 828, rt3: 12.9, 3,3'-t2: 331, and 3',5'-t2: 5.8. the corresponding renal clearances were in median (ml/min) t4: 31, t3: 133, rt3: 15, 3,3'-t2: 683, and 3',5'-t2: 4.5. the clearances differed mutually (p less ...19873591221
the effects of stimulus area and adaptation temperature on warm and heat pain thresholds in man.the effect of stimulus area and adaptation temperature on warm and heat pain threshold were studied in healthy human subjects using a contact thermal stimulator. the stimulus area was varied in the range of 1.3 to 11.8 cm. both the warm and heat pain thresholds decreased with increasing stimulus surface. heat pain and warm thresholds were equally affected by the stimulus surface. the adapting temperature was varied in the range 25-35 degrees c, and warm thresholds were elevated with increasing a ...19873596930
[studies on dynamic posture in normal persons and scoliotic patients].dynamic posture of 127 normal males, 144 normal females and 68 scoliotic patients was studied by a system utilizing moiré topography, a video recorder and apple ii microcomputer system. the normal group was divided into five subgroups by age and the scoliotic group into three subgroups according to the cobb angle. the following results were obtained: the clockwise rotation during on-the-spot marching was larger in early juvenile females. the rotation movement during on-the-spot marching was almo ...19873598288
does syphilis facilitate sexual acquisition of hiv? 19873599340
[reconsideration of the 'bacteriological examination' of slaughter stock].a comparative trial was made by six meat inspection services to attain uniform indications for the 'bacteriological examination' of slaughtered animals in accordance with the 'meat inspection regulations' of the dutch 'meat inspection act'. the results of the study compelled the investigators to scrutinize these regulations. the 'bacteriological examination' which is prescribed, is designed to exclude septicaemic animals from human consumption. applying the rules will result in condemnation of a ...19873603543
tunica media changes in the spontaneously hypertensive rat (shr).the histological, ultrastructural, morphometrical and histochemical aspects of the arterial media were studied in young and aged shr, and compared to normotensive wistar kyoto rats. the diffuse thickening was the most characteristic feature of the hypertensive media. it seems due to three processes: early generalized hypertrophy of smooth muscle cells (smc); connective matrix neogenesis and smc proliferation, more evident in peripheral vasculature. the present paper discusses the following hyper ...19873606728
node of ranvier formation along fibres regenerating through silicone tube implants: a freeze-fracture and thin-section electron microscopic study.thin-section and freeze-fracture electron microscopy have been used to examine the morphogenesis of the node of ranvier in peripheral nerves regenerating through silicone tubes. a major question posed by this study is whether node formation in fibres regenerating across a gap recapitulates that occurring in normal development. node formation occurs concurrently with myelination and follows a similar spatial gradient of progression from a proximal to distal direction along the regenerated nerve. ...19873612184
effects of intraneural injection of ricinus communis agglutinin-60 into rat vagus nerve.the dorsal motor nucleus (dmn) of the rat was studied at various survival periods following an intraneural injection of ricinus communis agglutinin-60 (rca-60) into the vagus nerve at the mid-cervical region. no obvious structural changes were noted in the dmn 2 and 4 days after the injection of rca-60. at 5 and 6 days after the rca-60 injection, the larger neurons (measuring 19 x 12 microns) in the dmn underwent chromatolytic degeneration whereas the smaller ones (measuring 10 x 6 microns), cha ...19873612185
[use of polypeptides from the vascular wall in the therapy of acute pancreatitis in rats in an experiment]. 19873615005
marginal mandibulectomy for malignant disease: indications, rationale, and results.the theoretical basis for performing marginal mandibular resection, as opposed to segmental resection, is discussed; and criteria are suggested. results are presented that suggest that, in the treatment of malignant disease around the jaw, although the mortality from other tobacco- and alcohol-related diseases remains high, good functional and cosmetic results can be achieved with no loss of local control.19873625019
bioequivalence and pharmacokinetic profile of promethazine hydrochloride suppositories in humans.a bioequivalence study of promethazine hydrochloride (10-[2-(dimethylamino)propyl]-phenothiazine monohydrochloride) was conducted in 20 male human subjects with the purpose of comparing, under blind condition, the human serum levels of promethazine in three different formulations. the formulations tested were a 50-mg promethazine hydrochloride polyethylene glycol suppository, a 50-mg promethazine hydrochloride cocoa butter-white wax suppository, and a 50-mg oral dose of promethazine hydrochlorid ...19873625487
bioequivalence and pharmacokinetic profile of promethazine hydrochloride suppositories in humans.a bioequivalence study of promethazine hydrochloride (10-[2-(dimethylamino)propyl]-phenothiazine monohydrochloride) was conducted in 20 male human subjects with the purpose of comparing, under blind condition, the human serum levels of promethazine in three different formulations. the formulations tested were a 50-mg promethazine hydrochloride polyethylene glycol suppository, a 50-mg promethazine hydrochloride cocoa butter-white wax suppository, and a 50-mg oral dose of promethazine hydrochlorid ...19873625487
a computerized system for measuring detection sensitivity over the visual field.a device is described for comprehensively measuring the capability of human operators to detect and react to visual signals occurring at a variety of locations throughout the functional visual field. the system is completely automated and computerized, and provides both documentation files and descriptive graphics of the operator's performance immediately upon completion of testing. sensitivity of detection can be measured for red, yellow, and green stimulus colors in a testing configuration whi ...19873632544
kaposi's sarcoma: a trifactorial model.to explain the epidemiology of kaposi's sarcoma, including its predominance in men, i present a theory based on three concurrent factors: immunodeficiency, exposure to a specific viral agent, and a recessive, highly prevalent x-linked regulatory gene. the x-linked locus is inferred from comparing the aberrant lymphaticovenous nature of immature lesions with absence of lymphaticovenous connections in xo karyotypes. the theory predicts that for heterosexual populations without sex differences in v ...19873647226
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