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a procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver.experimental conditions have been designed that permit both extracellular and intracellular immunolocalization of various collagen types and fibronectin in rat liver. the procedure involves paraformaldehyde fixation by perfusion of the organ, use of saponin as a membrane permeabilizing agent, and visualization of the matrix components by indirect immunoperoxidase. intracellular demonstration of collagens was particularly sensitive to the composition of the fixative and the duration of fixation. ...19853886779
[rare forms of yersinia infections in adults]. 19853887018
establishment of mics of moxalactam for control and reference anaerobic organisms in agar dilution and microdilution techniques.the mics of moxalactam were determined for eight national committee for clinical laboratory standards control and reference strains and for fusobacterium nucleatum atcc 10953 by agar and microdilution techniques. the recommended mic for the control strain bacteroides fragilis in both agar and microdilution tests is 0.5 micrograms/ml. recommended mics for bacteroides thetaiotaomicron atcc 29741 and clostridium perfringens atcc 13124 by agar dilution are 8 and 0.063 micrograms/ml, respectively. th ...19853888103
b/t cell ratio of rabbit peripheral blood lymphocytes. influence of separation technique on results.spontaneous in vitro t cell rosette formation at room temperature leading to enrichment of b cells has been reported. we tested 10 individual new zealand white rabbits sequentially, separating the lymphocytes either at room temperature or at 37 degrees c. t cells are lost constantly at room temperature but to a lesser extent at 37 degrees c. the determination of the yield of lymphocytes after ficoll separation gives the best control for the accuracy of the results. if lymphocytes are separated a ...19853889161
isolation of a spirochete from the soft tick, ornithodoros coriaceus: a possible agent of epizootic bovine abortion.a borrelia-like spirochete was detected in all three parasitic stages of ornithodoros coriaceus, the soft tick implicated in the epizoology of epizootic bovine abortion. after the spirochete had been isolated, its distinctness from other north american tick-borne borreliae as well as from spirochaeta aurantia, treponema pallidum, and leptospira interrogans serovar pomona was established on the basis of its morphology, protein components, and inability to infect mice. the spirochete is passed tra ...19853898367
alternative treatment of drug resistant falciparum malaria with particular reference to africa south of the sahara. 19853907533
an acoustic plethysmograph to measure total infant body volume.an acoustic plethysmograph intended to measure the body volume of premature infants has been developed using the principle of the helmholtz resonator, in which the resonance frequency is dependent on the volume of the resonating cavity. a prototype system was built and used to measure the volume of inanimate objects and newborn miniature pigs. results for inanimate objects agree within 1 percent with comparable measurements by water displacement. results of the animal body volume measurements co ...19853908815
cefamandole resistance transfer in bacterial strains from two newborn units.transfer of cefamandole resistance was demonstrated from strains of citrobacter freundii as well as from individual strains of enterobacter cloacae, acinetobacter anitratus and klebsiella pneumoniae isolated from patients in two newborn units. in citrobacter freundii, cefamandole resistance was transferred always with cephalotin resistance as well as with a tem-like beta lactamase (conferring resistance to ampicillin, carbenicillin and azlocillin). citrobacter freundii strains from hospital i we ...19853910723
the sfia11 mutation prevents filamentation in a response to cell wall damage only in a reca+ genetic background.n-alpha-palmitoyl-l-lysyl-l-lysine dihydrochloride ethyl ester (pll) at sublethal doses causes filamentous growth of e. coli strains except sfia mutants, which divide normally in its presence. pll does not elicit the sos responses as judged by lambda prophage induction, an increase of reca protein synthesis or induction of the sfia operon in a sfia::lacz fusion strain. thus, it appears that filamentation caused by pll is not an sos function and might be the result of membrane damage by pll, whic ...19853911030
health effects of nitrogen dioxide and oxidants.the document is an evaluation of the health effects of nitrogen dioxide (no2) and ozone (o3) intended to serve as a basis for establishing swedish air quality standards. the specific effects of nitrogen dioxide and ozone are reviewed on the basis of published studies on animals, tissues and cells, controlled studies on humans, and epidemiologic studies. the focus is on the importance of the gases in relation to sensitive groups, outdoor-indoor exposure relationships, the question of short- or lo ...19853911379
[effects of nitronaphthofurans on the transfer of a plasmid]. 19853915195
[aids to wound healing. from empirical tradition to a scientific approach]. 19853915246
immunology and immunopathology of malignant catarrhal fever. 19853917220
evidence of direct thyroid-stimulating action of thyrotropin-releasing hormone by perifusion of rat thyroid fragments.the possibility that trh has a direct thyroid-stimulating action has never been reported. a number of studies have shown a rise in serum concentrations of thyroid hormone after stimulation with trh, without a rise in tsh secretion. this has led us to test trh with rat thyroid fragment perifusion. significant t4 release was observed for trh concentrations as low as 1.7 x 10(-11) m. a dose-response curve was determined. the response was immediate, reaching a peak after the sixth minute and continu ...19853917904
biologic activity of type i and type ii fusobacterium nucleatum isolates from clinically characterized sites.fusobacterium nucleatum is a microorganism commonly cultured from periodontal disease sites. f. nucleatum isolates (120) were obtained from subgingival plaque samples taken from 27 clinically characterized sites using a selective culture medium. all isolates were verified by morphology, gram-stain reactions, oxygen tolerance and biochemical reactions. a total of eight clinical isolates and two typed strains were used for further evaluation. in this study, there was no relationship found between ...19853925111
inhibition of lymphocyte invasion by sporozoites and the transformation of trophozoite infected lymphocytes in vitro by serum from theileria annulata immune cattle.serum from calves immune to theileria annulata inhibited sporozoite-induced infection and transformation of peripheral blood leucocytes (pbl) into macroschizont infected lymphoblastoid cells in vitro. invasion by sporozoites was suppressed by some sera, especially that obtained after challenge, but in many instances suppression of infection could be attributed only to retardation of development of pbl infected with intracellular trophozoites into proliferating macroschizont infected cells. suppr ...19853925422
subcellular localization of vasopressin-like material in platelets.it has recently been reported that 90% of circulating vasopressin in humans appears to be in platelets. we have confirmed these results and studied the subcellular localization and secretion of vasopressin from human platelets. sucrose density gradient analysis showed highest relative specific activity for vasopressin in the membrane fractions. stirring of platelet-rich plasma with a number of different platelet-aggregating agents including epinephrine, norepinephrine, arachidonic acid, collagen ...19853928782
the establishment of reproducible, complex communities of oral bacteria in the chemostat using defined inocula.nine commonly isolated oral bacterial populations were inoculated into a glucose-limited and a glucose-excess (amino acid-limited) chemostat maintained at a constant ph 7.0 and a mean community generation time of 13.9 h. the bacterial populations were streptococcus mutans atcc 2-27351, strep. sanguis nctc 7865, strep. mitior ef 186, actinomyces viscosus wvu 627, lactobacillus casei ac 413, neisseria sp. a1078, veillonella alkalescens atcc 17745, bacteroides intermedius t 588 and fusobacterium nu ...19853932293
[calcium and magnesium ions in the septum and nucleus accumbens of the rabbit are antagonistic to acupuncture analgesia and morphine analgesia]. 19853939075
[calcium and magnesium ions in the septum and nucleus accumbens of the rabbit are antagonistic to acupuncture analgesia and morphine analgesia]. 19853939075
[riboflavin operon in bacillus subtilis contains additional promoters].using the methods of molecular cloning permitted to show that riboflavin operon of bacillus subtilis contains four promoters. three of them are functionally active in the bacillus subtilis system. the main promoter of the operon with regulatory region was cloned in plasmid ppl603. cells containing the constructed plasmid pgm32 are resistant to chloramphenicol. the level of resistance is regulated by concentration of riboflavin (the effector of operon). the following model of rib-operon has been ...19853939570
surface structures (peritrichous fibrils and tufts of fibrils) found on streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera.we screened 36 strains of streptococcus sanguis biotype i and 8 strains of s. sanguis biotype ii for the presence of surface structures and for their ability to coaggregate with actinomyces viscosus, actinomyces naeslundii, and fusobacterium nucleatum. negative staining under an electron microscope revealed detectable surface structures on all s. sanguis strains. the majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. they usually a ...19853965396
interface mechanics and bone growth into porous co-cr-mo alloy implants.the interface mechanics and bone growth into porous co-cr-mo alloy implants were evaluated. three mean pore sizes (155, 235, and 350 microns) were studied for implants fabricated with one, two, and three layers of spherical powder particles and totally porous implants. all implants had a pore volume of 38%-40%. ten implants were inserted transcortically in the femora of six adult mongrel dogs and were allowed to remain in situ for a period of 12 weeks. postretrieval mechanical testing and histol ...19853971631
long-term effects of q-switched ruby laser on monkey anterior chamber angle.in an attempt to clarify whether pulsed lasers might be able to cause permanent fistulas from the anterior chamber to the interior of the canal of schlemm, slightly suprathreshold, low energy, small diameter q-switched ruby laser pulses were applied to the trabecular meshwork of nine eyes of six rhesus monkeys. clinical examinations during the next 2 months disclosed no adverse effect on the cornea, iris, lens, or retina. there was transient mild inflammation in five eyes. intraocular pressure w ...19853972496
long-term effects of q-switched ruby laser on monkey anterior chamber angle.in an attempt to clarify whether pulsed lasers might be able to cause permanent fistulas from the anterior chamber to the interior of the canal of schlemm, slightly suprathreshold, low energy, small diameter q-switched ruby laser pulses were applied to the trabecular meshwork of nine eyes of six rhesus monkeys. clinical examinations during the next 2 months disclosed no adverse effect on the cornea, iris, lens, or retina. there was transient mild inflammation in five eyes. intraocular pressure w ...19853972496
neonatal lupus erythematosus. a report of three cases associated with anti-ro/ssa antibodies.three patients had neonatal lupus erythematosus syndrome. all three infants were girls who presented with characteristic skin lesions but without evidence of congenital heart block. all three mothers were asymptomatic at the time of delivery and have remained so until the present time. all mothers were positive for anti-ro/ssa antibodies, as were the infants prior to 6 months of age. anti-la/ssb antibodies were not present in either the mothers or the infants. hla typing revealed that two of the ...19853977359
descending lumbosacral cord potentials (dlcp) evoked by stimulation of the median nerve.in 22 normal human subjects, descending lumbosacral cord potentials (dlcp) were recorded intrathecally after stimulation of the median nerve at the elbow. the onset of dlcp is very short in latency (mean 12.1 ms) with a prominent sharp early positive peak (mean latency 13.7 ms) followed by a sharp negative peak (mean 17.6 ms). the amplitude of the first part of dlcp varied between 0.6 and 6.7 microv (mean 2.3 microv). the response was recorded most easily when the tip of intrathecal electrode wa ...19853978422
airgun injuries. 19853984193
[reciprocal regulation of growth and differentiation in hepatocytes by cell surface modulator and loss of regulation during carcinogenesis].in primary monolayer cultures of mature rat hepatocytes, cell growth and hepatocyte-specific functions were mediated by a cell surface component (named the cell surface modulator) via cell-cell contact. the modulator activity was heat-labile and trypsin-sensitive. activity was also found in plasma membranes from kidney, brain, lung, and erythrocytes. the modulator was solubilized by 4% octylglucoside plus 4m guanidine hcl from liver membranes. the molecular weight of the modulator was 670kd dete ...19853985639
teratogenic potential of inhaled dichlorobenzenes in rats and rabbits.orthodichlorobenzene (odcb) and paradichlorobenzene (pdcb) were evaluated for teratogenic potential in rats (odcb only) and rabbits. groups of bred rats and inseminated rabbits were exposed to 0, 100, 200, or 400 ppm of odcb; groups of inseminated rabbits were exposed to 0, 100, 300, or 800 ppm of pdcb. animals were exposed for 6 hr/day on days 6 through 15 (rats) or 6 through 18 (rabbits) of gestation. maternal toxicity, as evidenced by a significant decrease in body weight gain, was observed i ...19853987993
serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis.in the present study we sought to determine whether serum antibody was present against microorganisms which predominate in the subgingival flora of young adults with generalized severe periodontitis (sp). subjects with sp were often seropositive for eubacterium brachy, fusobacterium nucleatum e3c22, and peptostreptococcus micros, whereas subjects with juvenile periodontitis (jp) and subjects with healthy periodontium (hp) were not. both sp and jp subjects were more frequently seropositive for ba ...19853988337
microbial ecology of plaque in rats with naturally occurring gingivitis.the microbial ecology of adherent plaque was investigated in relation to the pathological findings of gingivitis in plaque-susceptible rats. plaque developed in the gingiva of the lower incisor in plaque-susceptible rats, but not in plaque-resistant rats, after they were fed a commercial powder diet. with increase in plaque volume, the total counts of bacteria increased 10(9) to 10(11)/g. in the first 3 months, bacteroides species increased and became the predominant population. streptococcus sp ...19853988345
sexual development in beef bulls following zeranol implants.two trials were conducted to study the effect of zeranol implants on growth and sexual development of bull calves. trial 1 compared the effects of implanting with 72 mg of zeranol at 48 d of age (branding), at 215 d of age, or at both times with nonimplanted control bulls. implanting at branding resulted in decreased scrotal circumference, testicle weight and proportion of bulls that could produce an ejaculate at 14 mo of age (p less than .01). implanting at 215 d of age had no effect on any of ...19853988627
relationship of rumen fluid dilution rate to rumen fermentation and dietary characteristics of beef steers.data from seven beef steer trials were compiled and regression analyses used to evaluate relationships among molar proportions of acetate (ac), propionate (pr) and butyrate (bu), total concentration of volatile fatty acids (vfa), rumen ammonia (nh3), rumen ph, rumen fluid dilution rate (fdr), rumen fluid volume (fvol), body weight (wt), dry matter intake (dmi) and dietary concentration and intake of crude protein (cp and cpi), acid detergent fiber (adf and adfi), ash (ash and ashi) and metaboliz ...19853988654
[histochemistry of hyaluronic acid of the bovine vitreous body by electronmicroscopy]. 19853993469
hypothermia produced by tributyl s,s,s-phosphorotrithioate (def).tributyl s,s,s-phosphorotrithioate (def) produces profound hypothermia in rats, mice and guinea pigs by inhibition of thermogenesis. its actions on heat conservation and motor control are, however, minimal. it is effective against both shivering and non-shivering thermogenesis and completely blocks the increase in body temperature evoked by anterior hypothalamic stimulation. a number of other measures indicated that this is unlikely to be due to a lack of peripheral thermogenic capacity: thus pl ...19853994512
acupuncture used as an adjunct in the treatment of a horse with tetanus. 19854004654
cytotoxic effects of culture supernatants of oral bacteria and various organic acids on vero cells.dilute culture filtrates of the three asaccharolytic black-pigmented bacteroides species as well as b. macacae, fusobacterium nucleatum, and animal strains resembling b. gingivalis were cytotoxic for the african green monkey kidney cell line (vero cells). butyric or propionic acid, major metabolic products of these bacteria, seem to be responsible for the characteristic morphological changes observed. among the organic acids studied, only butyric, propionic, and valeric acids exhibited cytotoxic ...19854005713
superinfected actinomycotic abscess of the liver.isolated actinomycotic liver abscess is rare. we report a case with fulminant evolution, which was treated successfully with surgery and penicillin. in human actinomycosis, actinomyces israeli is the main infective agent. an abdominal localisation is uncommon, occurring in less than 25% of cases. isolated liver lesions are extremely rare; their presence might be interpreted as the result of the hematogenous spread of a no longer present intestinal lesion. the evolution of actinomycosis is usuall ...19854019082
activity of a peptidyl prodrug, alafosfalin, against anaerobic bacteria.alafosfalin, an antibacterial phosphonodipeptide requiring peptide transport for activity, was tested for activity against clinical strains of anaerobic bacteria in peptide-free roche sensitivity test medium no. 5 agar. it was active against bacteroides spp., fusobacterium nucleatum, and clostridium perfringens but not against clostridium difficile. alafosfalin activity was antagonized by appropriate peptides. synergy was obtained with other cell wall-active antibiotics.19854026269
assessment of serum antibody patterns and analysis of subgingival microflora of members of a family with a high prevalence of early-onset periodontitis.in a study of members of a large family with a high prevalence of early-onset periodontitis, we sampled the subgingival microflora and characterized 40 isolates from each sample. we surveyed serum samples by enzyme-linked immunosorbent assay for antibodies reacting with any of a panel of 21 periodontal bacteria. the mother and 7 of her 13 children had early-onset periodontitis. bacteroides gingivalis was not detected in the subgingival flora of any affected or unaffected family member, and actin ...19854030102
clinical appearance of orofacial infections of odontogenic origin in relation to microbiological findings.fifty-eight patients with acute orofacial infections of odontogenic origin were classified into two groups with respect to the severity of infection. a total of 174 anaerobic and 22 aerobic bacterial strains were isolated. anaerobic gram-negative rods were isolated more frequently from the patients with severe infections than from the patients with infections judged as mild (p less than 0.05). the occurrence of fusobacterium nucleatum especially appeared to be associated with the severity of the ...19854031041
fatty acid composition and shwartzman activity of lipopolysaccharides from oral bacteria.the composition and the nature of the linkage of fatty acids and the shwartzman activity of lipopolysaccharide (lps) preparations derived from oral gram-negative bacteria including bacteroides gingivalis, bacteroides loesheii, eikenella corrodens, fusobacterium nucleatum, and actinobacillus actinomycetemcomitans were examined. 3-hydroxylated and nonhydroxy fatty acids of various chain lengths were found in all of the lps preparations. all nonhydroxy fatty acids were found to be ester-bound, and ...19854033466
differences in density and distribution of surface glycoconjugates between normal and dystrophic mouse schwann cells detected by statistical analyses of lectin-ferritin binding.cultures of schwann cells and neurons from dorsal root ganglia of normal (c57bl/6j +/+) and dystrophic (c57bl/6j dy2j/dy2j) mice were labeled with wheat germ agglutinin (wga) and ricinus communis agglutinin (rca-i) conjugated to ferritin. statistical methods were used to compare the regional densities and distribution characteristics of lectin binding in these two types of schwann cells, which differ in their capacities to ensheath and myelinate axons in vivo and in cultures. regional variations ...19854041887
aedes (gymnometopa) mediovittatus (diptera: culicidae), a potential maintenance vector of dengue viruses in puerto rico. 19854045943
fluoridation of drinking water: effects on kidney stone formation.the influence of fluoride in drinking water on stone formation was studied in animals and in "in vitro" crystallization experiments. in male wistar rats fluoride inhibits ethylenglycol induced calcification of the kidneys and stone formation. the in vitro results performed in synthetic urine exhibited a dose-dependent delay of crystal growth.19854049603
fluoridation of drinking water: effects on kidney stone formation.the influence of fluoride in drinking water on stone formation was studied in animals and in "in vitro" crystallization experiments. in male wistar rats fluoride inhibits ethylenglycol induced calcification of the kidneys and stone formation. the in vitro results performed in synthetic urine exhibited a dose-dependent delay of crystal growth.19854049603
oxygen transport in isolated cardiac myocytes.a preparation of isolated cardiac myocytes, retaining functional and structural properties of myocytes in the intact heart, has been used to measure intracellular oxygen pressure gradients. these are shallow and do not exceed 2 torr. oxygen does not limit mitochondrial function in the cardiac myocyte until intracellular myoglobin is almost entirely deoxygenated. most of the large oxygen pressure gradient in the working heart must be extracellular.19854062838
oxygen transport in isolated cardiac myocytes.a preparation of isolated cardiac myocytes, retaining functional and structural properties of myocytes in the intact heart, has been used to measure intracellular oxygen pressure gradients. these are shallow and do not exceed 2 torr. oxygen does not limit mitochondrial function in the cardiac myocyte until intracellular myoglobin is almost entirely deoxygenated. most of the large oxygen pressure gradient in the working heart must be extracellular.19854062838
kinetic independence of the subunits of cytosolic glutathione transferase from the rat.the steady-state kinetics of the dimeric glutathione transferases deviate from michaelis-menten kinetics, but have hyperbolic binding isotherms for substrates and products of the enzymic reaction. the possibility of subunit interactions during catalysis as an explanation for the rate behaviour was investigated by use of rat isoenzymes composed of subunits 1, 2, 3 and 4, which have distinct substrate specificities. the kinetic parameter kcat./km was determined with 1-chloro-2,4-dinitrobenzene, 4- ...19854062896
reversible expression of flagella in campylobacter jejuni.campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype.19854066041
evaluation of amniotic fluid in preterm labor with intact membranes.amniotic fluid was obtained from 35 pregnant women in preterm labor with intact membranes. their gestational ages ranged from 24 to 34 weeks. bacteria were detected in only 1 (3%) of the 35 amniotic fluid samples. the anaerobic culture grew bacteroides corrodens and fusobacterium nucleatum. the l/s ratio was greater than 4.0 in six patients (17%), 2.5-4.0 in two (6%) and less than 2.5 in 23 (66%); the quantity of fluid was inadequate for l/s analysis in four (11%). none of the 35 newborns develo ...19854067950
[analgesic effect, tolerance development and dependence potential of d-phenylalanine].in comparison to well-established non-narcotic analgetics, the amino acid d-phenylalanine produces similar, dose-dependent analgesic effects in animal experiments, but acts significantly longer. neither a tolerance of mice to d-phe after administration for 10 d nor a development of drug dependence in rats following treatment for 6 weeks could be regarded.19854070349
oral papillomas in cattle. 19854072491
interaction of dna with tilorone derivatives: viscometric investigations.interaction of dna with tilorone and six of its derivatives was studied in solution. this was done by viscosity measurements on both low and high molecular weight calf thymus dna, in order to interpret the experimental results in terms of changes of dna contour length (intercalation) and of persistence length (stiffening and bending). qualitative and semiquantitative analysis delivered the following results: the carbonyl derivatives of tilorone bind more strongly to dna than tilorone itself. the ...19854084265
self reactive delayed type hypersensitivity (dth) induced in mice by syngeneic lymphoblasts.normal and x-irradiated a mice injected with syngeneic concanavalin a (con a)-induced lymphoblasts revealed after challenge with syngeneic lipopolysaccharide (lps)-induced lymphoblasts delayed type hypersensitivity (dth) measured both by footpad swelling and by 125iudr accumulation. mice injected with allogeneic con a-induced lymphoblasts and challenged with syngeneic lps-induced lymphoblasts or vice versa, also generated an appreciable dth response. in contrast, con a-induced blast cells of hum ...19852580776
leukocyte tubuloreticular inclusions in reye's syndrome.tubuloreticular inclusions (tri) have been observed in the rough endoplasmic reticulum of blood lymphocytes and monocytes in two cases of reye's syndrome initiated by influenza infections. tubuloreticular inclusions are seen in these mononuclear leukocytes during the acute phase of illness, but not during convalescence. since tri have been demonstrated in peripheral mononuclear leukocytes in patients with acquired immunodeficiency syndrome, systemic lupus erythematosus, and certain viral infecti ...19852581524
lipid keratopathy in rabbits. an animal model system.rabbits rendered hypercholesterolemic on a high cholesterol diet while subjected to corneal suture placement to induce neovascularization made useful models for qualitatively and quantitatively studying lipid keratopathy. forty rabbit eyes were subjected to placement of four sutures located between 1 and 4 mm from the limbus. the neovasculature grew at a constant rate of 0.24 mm/day, unaffected by serum cholesterol levels. rabbits fed cholesterol prior to surgery were the earliest to display lip ...19852581537
myoglobin in the heart tissue of fishes lacking hemoglobin.myoglobin has been identified in the heart tissue of three species of antarctic icefish, chaenocephalus aceratus, pseudochaenichthys georgianus and chaenodraco wilsoni. quantitative analysis shows myoglobin concentrations that are substantially lower than other teleost fish. a simple and accurate method for the direct measurement of myoglobin in tissue is described.19852863077
[limitation of phage multiplication by microbes of the genus bordetella].possible causes limiting the multiplication of bordetella phages or inducing their restriction, such as the influence of lysogenic immunity and the restriction-modification (r-m) system or the incompatibility of the receptor apparatus, have been studied. the limitation of the multiplication of phages by some b. bronchiseptica and b. pertussis strains has been shown to be due to the presence of the r-m system and lysogenic immunity. in five b. bronchiseptica strains and two b. pertussis strains s ...19852868594
modulation of hla-dr antigens in the gingival epithelium in vitro by heat-killed fusobacterium nucleatum and e. coli lipopolysaccharide.the in vitro influence of the periodontopathic organism fusobacterium nucleatum (fn) on gingival tissue was examined using an organ culture system. treatment of gingival explants obtained from periodontally diseased sites with suspensions of fn, stimulated the expression of hla-dr antigens by langerhans cells (lc) in a dose-dependent fashion, and produced a maintenance of the lc markers t6 and atpase. similar effects were seen when e. coli lipopolysaccharide (lps) was substituted for suspensions ...19852414424
specificity of monoclonal antibodies reactive with fusobacterium nucleatum: effect of formalin fixation.monoclonal antibody specific for fusobacterium nucleatum was reacted with untreated and formalin fixed f. nucleatum cells by an enzyme-linked immunosorbent assay (elisa) and by indirect immunofluorescence. treatment of bacterial cells with formalin destroyed the antigenic determinant responsible for reactivity with this monoclonal antibody in both assays. formalin fixation had no effect on hemagglutination activity (ha) of f. nucleatum cells or reactivity with polyvalent rabbit antiserum in doub ...19852415637
target size of ca-pumps in pig coronary artery smooth muscle.the target sizes of the oxalate-independent ca uptake by the plasma membrane enriched fraction f2, and the oxalate-stimulated ca uptake by a fraction f3 slightly enriched in the endoplasmic reticulum were determined by radiation inactivation. the oxalate-independent ca uptake was inactivated with a d37 value of 1.96 +/- 0.30 mrad but the oxalate-stimulated ca uptake had a d37 value of 0.45 +/- 0.07 mrad. thus, in the smooth muscle the oxalate-stimulated ca uptake appeared to be due to a structur ...19852415797
[occupational dermatomycoses of zoophilic origin in bulgaria].the relative proportion of zoophilic dermatomycoses is increased in comparison with tinea capitis et pedum. the most frequent zoophilic species now is not only t. mentagrophytes, but t. verrucosum as well. occupational mycoses caused by t. mentagrophytes and t. quinckeanum in vivaria put forward new problems for experimental medicine. the author states his arguments in favour of t. quinckeanum as a separate, specialized species with reduced morphology, forming scutula. t. verrucosum is phylogene ...19852933238
thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles.the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ...19852934902
detection of dihydroorotate dehydrogenase activity as a marker of de novo nucleic acid synthesis in germinal centers of spleens following antigen administration.a histochemical technique, developed for the localization of dho-d activity in tissue, has been utilized to study the distribution of this activity in rabbit spleen. this enzyme is involved in the de novo synthesis of pyrimidines of nucleic acids via orotic acid as an intermediate. the enzyme was found to be most active at the periphery of follicles in the resting spleen. following antigenic challenge, enzyme activity became prominent for several days in macrophages within germinal centers, but ...19853023585
free radicals and myocardial ischemia. the role of xanthine oxidase.recent studies have established a major role for oxygen-derived free radicals in post ischemic tissue injury to the intestine. during ischemia, there appears to be a calcium-triggered, protease-dependent conversion of the native xanthine dehydrogenase to a superoxide-producing xanthine oxidase. the catabolic degradation of atp during ischemia provides an oxidizable substrate, hypoxanthine. on reperfusion, molecular oxygen is resupplied and a burst of superoxide production ensues, resulting in ex ...19852982206
modification of neurotransmitter receptor sensitivity in cat visual cortex during the critical period.we have examined the characteristics of various receptors in cat visual cortex during postnatal development. these included beta-adrenergic, gaba, benzodiazepine and acetylcholine receptors. for each population of receptor the number (bmax) and affinity (kd) were examined as a function of postnatal age (3 days-adult). for all receptors examined, the bmax increased during development from low early values to a peak within the critical period. the kd also changed during development for most recept ...19852994853
expression of amplified human beta interferon genes using heavy metal induction in chinese hamster ovary cells.an expression cassette consisting of the human beta-interferon (beta-ifn) cdna fused to the human metallothionein (met)iia promoter has been linked to a selectable mouse dihydrofolate reductase gene (dhfr) and used to transform dhfr-deficient chinese hamster ovary (cho) cells. transformants resistant to increasing concentrations of methotrexate (mtx) were isolated and found to secrete beta-ifn either constitutively or upon induction with cadmium (up to 325 000 units beta-ifn/10(6) cells/24 h). m ...19852996984
nucleotide sequence of the paer7 restriction/modification system and partial characterization of its protein products.bal31 deletion experiments on clones of the paer7 restriction-modification system from pseudomonas aeruginosa demonstrate that it is arranged as an operon, with the methylase gene preceding the endonuclease gene. the dna sequence of this operon agrees with in vitro transcription-translation assays which predict proteins of 532 amino acids, mr = 59,260 daltons, and 246 amino acids, mr = 27,280 daltons, coincident with the methylase and endonuclease genes, respectively. these predicted values coin ...19853001639
platelet-activating factor-binding protein in human serum.the occurrence of a kind of protein (pbp) with stronger paf-binding ability than albumin was shown in human serum. the molecular weight of pbp was approximately 160k to 180k. pbp seemed to be a pentamer composed of five 32k subunits. paf bound to pbp revealed platelet aggregation and was resistant to acetyl-hydrolase. paf synthesized in neutrophils stimulated with the ionophore a23187 was released by pbp from cells.19853004155
microbe-induced lymphocyte blastogenesis enhancement after preculture.the in vitro blastogenic response of human peripheral blood mononuclear cells to fusobacterium nucleatum and other oral microorganisms was enhanced if the peripheral blood mononuclear cells were cultured for 24 h at 37 degrees c prior to the addition of stimulant. the enhancement which occurred at optimal and supraoptimal concentrations of f. nucleatum (10 to 100 micrograms/ml) was detected after a preculture period of as little as 2 h. the blastogenic response was a result of t-cell proliferati ...19853156816
human and bovine endothelial cells synthesize membrane proteins similar to human platelet glycoproteins iib and iiia.human umbilical vein endothelial (huve) and bovine aortic endothelial (bae) cells in culture were examined to determine whether membrane proteins similar to human platelet glycoproteins (gp) iib and iiia were present. the huve and bae cells were either 125i-surface labeled or metabolically labeled. triton x-100 lysates of labeled cells were immunoprecipitated with polyclonal antibodies prepared against purified human platelet gp iib-iiia complex. two membrane proteins were detected on both huve ...19853161885
[studies of the mechanism of bacterial inactivation after administration of the preparation polchlor k].the purpose of the study was to determine the effect of chloroiodic complex compound with non-ionic surface active substances such as polchlor k on the kinetics of dying, activity of bacterial dehydrogenases and glucosidases as well as ultrastructure of bacterial cells on the example of escherichia coli and staphylococcus aureus. the evaluation of the kinetics of bacteria dying under the preparation activity revealed higher activity against e. coli than s. aureus. it was found that the preparati ...19863325944
neural mechanisms of binocular vision. 19863303676
infectious and sexually transmitted diseases: implications for dental public health.the acquired immunodeficiency syndrome (aids) has stimulated renewed attention toward infectious diseases and dental public health. currently, aids is defined as individuals with kaposi's sarcoma and/or pneumocystis carinii pneumonia and/or other life-threatening opportunistic infections (e.g., specific forms of tuberculosis or meningitis, candidal esophagitis), and associated immunosuppression that cannot be accounted for by another disease process and/or medications. as of january 1986, the ai ...19863457189
desulfuration of cysteine and methionine by fusobacterium nucleatum.fusobacterium nucleatum is a gram-negative anaerobic rod-shaped bacterium frequently isolated from human dental plaque. it is capable of the desulfuration of cysteine and methionine, resulting in the formation of sulfide and thiol volatiles, respectively. intact cells, as well as cell-free extracts produced by french pressure cell lysis of f. nucleatum, hydrolyzed radiolabeled cysteine to produce sulfide, pyruvic acid, and ammonia. the hydrolysis products of radiolabeled methionine were a volati ...19863458742
frequency distributions of periodontal attachment loss. clinical and microbiological features.the present investigation attempted to determine if the pattern of past periodontal destruction could be concisely summarized, and related to other clinical and microbiological parameters. 61 subjects between the ages of 12 and 61 years with destructive periodontal disease were evaluated at 6 sites per tooth for redness, plaque, suppuration, bleeding on probing, pocket depth, and attachment level. the frequency distribution of baseline attachment level measurements was computed for each individu ...19863462210
ethology in animal quarters.this contribution will be concerned with the interaction between environment, adaptability optimization and behaviour. animal laboratory experiments demand repeated measurements under identical environmental conditions. this is a prerequisite for the conventional statistical methodology used in order to clarify causal relationships involved in various biological functions. the understanding of biological functions is a necessary fundament for knowledge to prevent illness and to achieve a palliat ...19863469882
a novel type of human papillomavirus associated with genital neoplasias.the role of human papillomaviruses (hpvs) in the development of genital neoplasias has been well documented. the genomes of two hpv types, hpv16 and hpv18, have been found to be associated with about 70% of invasive carcinomas of the uterine cervix. as, under non-stringent hybridization conditions, hpv dna sequences have been detected in about 90% of cervical carcinomas, it seems likely that additional hpv types are associated with these tumours. here we report the molecular cloning and characte ...19863012352
a second post-transcriptional trans-activator gene required for htlv-iii replication.evidence is provided for the existence of a seventh gene in the genome of human t-lymphotropic virus type iii/lymphadenopathy-associated virus. the gene is necessary for replication and acts post-transcriptionally to relieve negative regulation of the messenger rna for the virion capsid and envelope proteins. these observations suggest mechanisms for regulating both the latent and lytic phases of the virus life cycle.19863012355
coagglutination reactions between candida albicans and oral bacteria.an agglutination assay for detecting intermicrobial adherence between the cells of candida albicans and various oral bacteria is described. strains of streptococcus sanguis, s. salivarius, s. mutans, s. mitis, fusobacterium nucleatum and actinomyces viscosus all coagglutinated with c. albicans. no interaction could be demonstrated between the cells of bacteroides melaninogenicus and those of c. albicans. preliminary investigations of these interactions suggest that binding of f. nucleatum and a. ...19863018251
chromosome damage induced by herpes simplex virus type 1 in early infection.the chromosome damage induced by herpes simplex virus type 1 (hsv-1) in vitro was examined up to 6 h after infection using ht-1080 cells. initial damage occurring within 3 h was specific, involving uncoiling of chromosome 1q12-21 and to a lesser extent the pericentric regions of chromosomes 9, 16 and satellited chromosomes. for the initial unwinding, synthesis of the immediate early class of hsv proteins needed to occur as was demonstrated using hsv-1 temperature-sensitive mutants tsk and tsb7 a ...19863020163
[nuclear magnetic resonance spectroscopy in medicine: biochemistry of intact tissues]. 19863020443
[nuclear magnetic resonance spectroscopy in medicine: biochemistry of intact tissues]. 19863020443
a computer-aided analysis of the effect of peripheral nerve transection on tmpase activity of substantia gelatinosa rolandi. 19863020818
methylcholanthrene: a possible pseudosubstrate for adrenocortical 17 alpha-hydroxylase and aryl hydrocarbon hydroxylase.in cultured bovine adrenocortical cells, loss of 17 alpha-hydroxylase activity was observed after incubation with 3-methylcholanthrene (3-mc). the suppression of 17 alpha-hydroxylase by 3-mc was rapid (50% loss of activity in 10 hr at 1 microm 3-mc), did not exhibit a lag period, and was not affected by cycloheximide. direct effects of 3-mc on 17 alpha-hydroxylase were observed only at high concentrations, but the concentration for 50% loss of activity was 0.3 microm when 3-mc was added for 24 h ...19863021165
regulation of guinea-pig immune functions by interleukin 2: critical role of natural killer activity in acute hsv-2 genital infection.we have previously demonstrated that recombinant interleukin 2 (ril 2) has a protective effect against acute hsv-2 infection in guinea pigs with a biphasic dose response which peaked between 4 and 20 x 10(4) u/kg, whereas 8 x 10(5) u/kg showed no effect on disease. animals that escaped infection appeared lack immunologic memory to hsv-2, suggesting a nonspecific immune mechanism. in this study we have found that nk activity of fresh splenocytes measured against hsv-2 infected human foreskin fibr ...19863021853
1,25 dihydroxyvitamin d increases hepatocyte cytosolic calcium levels. a potential regulator of vitamin d-25-hydroxylase.1,25 dihydroxyvitamin d (1,25(oh)2d) has been demonstrated to inhibit hepatic 25 hydroxyvitamin d (25 ohd) production. changes in cytosolic calcium have been shown to regulate cellular processes. using the fluorescent dye quin 2, we have investigated the effects of 1,25(oh)2d and 24,25(oh)2d on cytosolic calcium levels in hepatocytes. 1,25(oh)2d exposure for 5 min increases cytosolic calcium levels by 24% at a concentration of 100 pg/ml, 39% at a concentration of 1 ng/ml, and 50% at a concentrat ...19863084563
chemiluminescence in the assessment of polymorphonuclear leukocyte function in chronic inflammatory periodontal disease.polymorphonuclear neutrophils (pmn's) constitute the primary host resistance factor against infection. they are prominent cells in both the gingival tissue and gingival sulcus in most forms of periodontal disease. although defective pmn function has been implicated in the pathogenesis of localized juvenile periodontitis (ljp) and rapidly progressive periodontitis (rpp), this may not necessarily be the case in adult periodontitis (ap). a number of studies have failed to detect pmn dysfunction in ...19863084735
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...19863085110
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...19863085110
[unwanted modification of the thyroid gland by drugs with special reference to nonsteroidal antirheumatic agents].in two retrospective clinical studies was investigated the influence of the modern non-steroidal antirheumatic drugs indometacin and diclofenac (rewodina) on the thyroid gland and corresponding peripheral hormone parameters. under longterm treatment with indometacin a moderate strumigenic effect could be observed, which could not clearly be proved under the diclofenac therapy. in all patients with rheumatoid arthritis, independent of the kind of pharmacotherapy, decreased t3-hormone levels were ...19863087075
influence of glucocorticoids on calcium absorption in different segments of the rat intestine.glucocorticoids enhance the movement of fluid and sodium in the duodenum, thereby resulting in an increase in the passive transport of calcium. since passive transport of calcium predominates in the distal intestinal segments, the influence of glucocorticoids on calcium and fluid transport in the duodenum, mid-jejunum, ileum, and colon of the rat was studied. calcium transport and fluid absorption was determined by the in vivo ligated loop technique. one segment was ligated in each animal. under ...19863087601
hypophysectomy does not alter the increase in striatal dopamine receptor density produced by 6-hydroxydopamine lesions in male rats.while hypophysectomy can alter the increase in striatal dopamine (da) receptor density observed after estrogen, haloperidol or prolactin treatments, it does not alter the increase in density found after 6-hydroxydopamine lesions of the da neurons in male rats. this observation suggests that striatal da receptors can be regulated by at least two distinct mechanisms, only one of which requires the availability of pituitary hormones.19863092152
emergence of beta-lactamase producing anaerobic bacteria in the tonsils during penicillin treatment.the emergence of beta-lactamase producing bacteria in the microflora in the oropharyngeal cavity was studied in ten healthy volunteers treated with 1 g phenoxymethylpenicillin b.i.d. for ten days. beta-lactamase activity in saliva was also investigated. a significant increase in the number of beta-lactamase producing strains of bacteroides species and fusobacterium nucleatum was observed. one beta-lactamase producing staphylococcus aureus strain was recovered in one of the volunteers before the ...19863093222
characterization of benzodiazepine receptors in the bovine pineal gland: evidence for the presence of an atypical binding site.bovine and rat pineal benzodiazepine receptors were characterized using ligands with high affinities for either 'central-type' (cbr) or 'peripheral-type' (pbr) benzodiazepine receptors. the characteristics (bmax = 83 +/- 10 fmol/mg protein, kd = 3.88 +/- 0.46 nm) of benzodiazepine receptors in bovine pineal membranes measured with [3h]flunitrazepam (using flunitrazepam to define non-specific binding) were consistent with previously reported values. however, if non-specific binding was defined us ...19863024779
characterization of benzodiazepine receptors in the bovine pineal gland: evidence for the presence of an atypical binding site.bovine and rat pineal benzodiazepine receptors were characterized using ligands with high affinities for either 'central-type' (cbr) or 'peripheral-type' (pbr) benzodiazepine receptors. the characteristics (bmax = 83 +/- 10 fmol/mg protein, kd = 3.88 +/- 0.46 nm) of benzodiazepine receptors in bovine pineal membranes measured with [3h]flunitrazepam (using flunitrazepam to define non-specific binding) were consistent with previously reported values. however, if non-specific binding was defined us ...19863024779
[immunobiological activity of 41k protein (porin) derived from the cell envelope of fusobacterium nucleatum]. 19863025315
ro 15-1788 suppresses the development of kindling through the benzodiazepine receptor.beta-carboline (norharman) has been shown to produce kindled seizures when given systemically for long periods of time. the expression of the kindled seizure activity can be blocked by ligands of the benzodiazepine receptor suggesting the receptor as a site of vulnerability in the kindling mechanism. the present data show that ro 15-1788, a receptor antagonist, suppresses the development of kindled seizures as demonstrated by the delayed appearance of each behavioral stage and the decreased seve ...19863026559
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