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effects of cranberry components on human aggressive periodontitis gingival fibroblasts.aggressive periodontitis (agp) causes rapid periodontal breakdown involving agp gingival fibroblast production of cytokines [i.e. interleukin (il)-6, a bone metabolism regulator], and matrix metalloproteinase (mmp)-3. lipopolysaccharide upregulates fibroblast il-6 and mmp-3, via transcription factors (i.e. nf-κb). cranberry (vaccinium macrocarpon) inhibits lipopolysaccharide-stimulated macrophage and normal gingival fibroblast activities, but little is known of its effects on agp fibroblasts. ob ...201323106206
draft genome sequence of fusobacterium nucleatum chdc f128, isolated from a periodontitis lesion.fusobacterium nucleatum is classified into five subspecies. f. nucleatum chdc f128 was isolated from a periodontitis lesion and proposed as a new subspecies based on the comparison of the nucleotide sequences of the rna polymerase beta subunit and zinc protease genes. here, we report the draft genome sequence of the strain.201223105064
inflammatory index and treatment of brain abscess.this study retrospectively analyzed 12 patients with brain abscesses. half of the patients were diagnosed inaccurately in the initial stage, and 7.2 days were required to achieve the final diagnosis of brain abscess. the patients presented only with a moderately elevated leukocyte count, serum crp levels, or body temperatures during the initial stage. these markers changed, first with an increase in the leukocyte count, followed by the crp and body temperature. the degree of elevation tended to ...201223092104
antibacterial activity of calcium hydroxide and chlorhexidine containing points against fusobacterium nucleatum and parvimonas micra.up to 90% of the flora of an infected root canal consists of obligate anaerobic bacteria. however, most studies have centred on microaerophiles. this quantitative assay investigated the antibacterial effects against fusobacterium nucleatum and parvimonas micra of gutta-percha (gp) points containing either calcium hydroxide (ca(oh)(2)) or chlorhexidine (chx) compared to those of conventional gp points.201223077425
antibacterial activity of myristica fragrans against oral pathogens.myristica fragrans houtt is mostly cultivated for spices in penang island, malaysia. the ethyl acetate and ethanol extracts of flesh, mace and seed of myristica fragrans was evaluated the bactericidal potential against three gram-positive cariogenic bacteria (streptococcus mutans atcc 25175, streptococcus mitis atcc 6249, and streptococcus salivarius atcc 13419) and three gram-negative periodontopathic bacteria (aggregatibacter actinomycetemcomitans atcc 29522, porphyromonas gingivalis atcc 3327 ...201223049613
microscope-based imaging platform for large-scale analysis of oral biofilms.a microscopic method for noninvasively monitoring oral biofilms at the macroscale was developed to describe the spatial distribution of biofilms of different bacterial composition on bovine enamel surfaces (bes). for this purpose, oral biofilm was grown in situ on bes that were fixed at approximal sites of individual upper jaw acrylic devices worn by a volunteer for 3 or 5 days. eubacteria, streptococcus spp., and fusobacterium nucleatum were stained using specific fluorescence in situ hybridiza ...201223042171
porphyromonas gingivalis entry into gingival epithelial cells modulated by fusobacterium nucleatum is dependent on lipid rafts.host cell invasion by a major periodontal pathogen, porphyromonas gingivalis, has been proposed as an important mechanism involved in host-pathogen interactions in periodontal and cardiovascular diseases. the present study sought to gain insight into the underlying mechanism(s) involved in previously demonstrated fusobacterial modulation of host cell invasion by p. gingivalis. an immortalized human gingival cell line ca9-22 was dually infected with p. gingivalis atcc 33277 and fusobacterium nucl ...201223034475
protective potential of non-dialyzable material fraction of cranberry juice on the virulence of p. gingivalis and f. nucleatum mixed infection.periodontitis is a polymicrobial infectious disease. a novel potential chemical treatment modality may lie in bacterial anti-adhesive materials, such as cranberry juice fractions. the aim of this study is to explore the effect of high molecular weight cranberry constituent (non-dialyzable material [ndm]) on the virulence of a mixed infection with porphyromonas gingivalis and fusobacterium nucleatum in mice.201323030239
morphological, biochemical, physiological and molecular aspects of the response of fusobacterium nucleatum exposed to subinhibitory concentrations of antimicrobials.subinhibitory concentrations (sics) of antimicrobials may result in alterations in bacterial biology with implications for its potential aggression. this has considerable importance for the resident microbiota. our aim was to analyze the effects of sics of antimicrobials on the morphological, biochemical, physiological and molecular characteristics of the resident anaerobic fusobacterium nucleatum. fourteen strains were obtained from f. nucleatum atcc 25586, selected by culturing on sics of ampi ...201223023263
detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women.the aim of this study was to use the fluorescence in situ hybridization (fish) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. this cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque samples were collected and the fish ...201223018230
maternal oral origin of fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16s-23s rrna gene intergenic transcribed spacer region.fusobacterium nucleatum, a common gram-negative anaerobe prevalent in the oral cavity, possesses the ability to colonize the amniotic cavity and the fetus. however, f. nucleatum may also be part of the vaginal microbiota from where it could reach the amniotic tissues. due to the heterogeneity of f. nucleatum, consisting of five subspecies, analysis at the subspecies/strain level is desirable to determine its precise origin. the aims of this study were: (i) to evaluate the use of the 16s-23s rrna ...201323002071
proteomics of streptococcus gordonii within a model developing oral microbial community.streptococcus gordonii is one of several species that can initiate the formation of oral biofilms that develop into the complex multispecies microbial communities referred to as dental plaque. it is in the context of dental plaque that periodontal pathogens such as porphyromonas gingivalis cause disease. we have previously reported a whole cell quantitative proteomics investigation of p. gingivalis in a model dental plaque community of s. gordonii, p. gingivalis, and fusobacterium nucleatum. her ...201222989070
isothermal microcalorimetry provides new insights into biofilm variability and dynamics.the purpose of this study was to investigate a three-species in vitro biofilm with peri-implantitis-related bacteria for its variability and metabolic activity. streptococcus sanguinis, fusobacterium nucleatum, and porphyromonas gingivalis were suspended in simulated body fluid containing 0.2% glucose to form biofilms on polished, protein-coated implant-grade titanium disks over 72 h using a flow chamber system. thereafter, biofilm-coated disks were characterized by scanning electron microscopy ...201222967269
draft genome sequence of fusobacterium nucleatum subsp. fusiforme atcc 51190t.fusobacterium nucleatum, one of the major causative bacteria of periodontitis, is classified into five subspecies (nucleatum, polymorphum, vincentii, animalis, and fusiforme) on the basis of the several phenotypic characteristics and dna homology. this is the first report of the draft genome sequence of f. nucleatum subsp. fusiforme atcc 51190(t).201222965077
a proteomic investigation of fusobacterium nucleatum alkaline-induced biofilms.the gram negative anaerobe fusobacterium nucleatum has been implicated in the aetiology of periodontal diseases. although frequently isolated from healthy dental plaque, its numbers and proportion increase in plaque associated with disease. one of the significant physico-chemical changes in the diseased gingival sulcus is increased environmental ph. when grown under controlled conditions in our laboratory, f. nucleatum subspecies polymorphum formed mono-culture biofilms when cultured at ph 8.2. ...201222943491
ascorbate and α-tocopherol differentially modulate reactive oxygen species generation by neutrophils in response to fcγr and tlr agonists.periodontitis, a ubiquitous chronic inflammatory disease, is associated with reduced antioxidant defences and neutrophil hyperactivity in terms of reactive oxygen species (ros) generation. its phenotype is thus characterized by oxidative stress. we have determined the effect of antioxidant micronutrients ascorbate and α-tocopherol on neutrophil ros generation. peripheral neutrophils from periodontally-healthy individuals (n = 20) were challenged with phorbol myristate acetate, igg-opsonised stap ...201322914919
bacterial antagonism against periodontopathogens.the aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria.201322897652
micronutrient modulation of nf-κb in oral keratinocytes exposed to periodontal bacteria.chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. we have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including nf-κb and cytokine signalling, when exposed to the periodontal bacteria porphyromonas gingivalis and fusobacterium nucleatum in vitro. the purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro ...201322890546
antibiotic susceptibility profiles of oral pathogens.periodontitis is a bacterial disease that can be treated with systemic antibiotics. the aim of this study was to establish the antibiotic susceptibility profiles of five periodontal pathogens to six commonly used antibiotics in periodontics. a total of 247 periodontal bacterial isolates were tested for susceptibility to the six antibiotics using the etest method. mic(50) and mic(90) values (minimum inhibitory concentrations for 50% and 90% of the organisms, respectively) were calculated. both eu ...201222890195
periodontal pathogen load and increased antibody response to heat shock protein 60 in patients with cardiovascular disease.to determine the relationship between periodontal pathogen load and anti-human heat shock protein 60 (hhsp60) antibodies in patients with established cardiovascular disease (cvd).201222882677
fusobacterium nucleatum infection mimicking metastatic cancer.a 48-year-old man presented with fevers, chills, weight loss, multiple liver masses, and several superficial and deep venous thromboses in lower extremities. cancer work up was negative. a liver biopsy grew fusobacterium nucleatum. to our knowledge, f. nucleatum infection presenting with multiple liver masses and trousseau-like syndrome has not been reported earlier.201222875742
porphyromonas gingivalis mediates inflammasome repression in polymicrobial cultures through a novel mechanism involving reduced endocytosis.the interleukin (il)-1β-processing inflammasome has recently been identified as a target for pathogenic evasion of the inflammatory response by a number of bacteria and viruses. we postulated that the periodontal pathogen, porphyromonas gingivalis may suppress the inflammasome as a mechanism for its low immunogenicity and pathogenic synergy with other, more highly immunogenic periodontal bacteria. our results show that p. gingivalis lacks signaling capability for the activation of the inflammaso ...201222843689
osteomyelitis of a long bone due to fusobacterium nucleatum and actinomyces meyeri in an immunocompetent adult: a case report and literature review.fusobacterium species are uncommon causes of osteomyelitis. these organisms are normal flora of the oral cavity. therefore, they mostly cause osteomyelitis of the head and neck. hematogenous osteomyelitis at distant sites other than the head and neck has rarely been reported in pediatric or immunocompromised patients. here, we report the first case of osteomyelitis of a long bone combined with a muscle abscess due to fusobacterium nucleatum in an otherwise healthy adult.201222817336
antibacterial effects of blackberry extract target periodontopathogens.antimicrobial agents provide valuable adjunctive therapy for the prevention and the control of oral diseases. limitations in their prolonged use have stimulated the search for new, naturally occurring agents with more specific activity and fewer adverse effects. here we sought to determine the antibacterial properties of blackberry extract (bbe) in vitro against oral bacterial commensals and periodontopathogens.201222812456
efficacy of berberine, an antimicrobial plant alkaloid, as an endodontic irrigant against a mixed-culture biofilm in an in vitro tooth model.berberine, a plant alkaloid isolated from many medicinal plants, has shown antimicrobial activity against selected oral pathogens. the purpose of this investigation was to evaluate the antimicrobial efficacy of berberine solution against selected endodontic pathogens using a multispecies biofilm tooth model.201222794217
comparison of endotoxin levels found in primary and secondary endodontic infections.this clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides [lpss]) found in primary and secondary endodontic infections with apical periodontitis by correlating lps contents with clinical/radiographic findings. in addition, the presence of target gram-negative anaerobic bacteria was also investigated.201222794210
effectiveness of egg yolk immunoglobulin (igy) against periodontal disease-causing fusobacterium nucleatum.to evaluate the in vitro and in vivo effectiveness of egg yolk immunoglobulin (igy) against periodontal disease-causing fusobacterium nucleatum.201222789022
strain-specific colonization patterns and serum modulation of multi-species oral biofilm development.periodontitis results from an ecological shift in the composition of subgingival biofilms. subgingival community maturation is modulated by inter-organismal interactions and the relationship of communities with the host. in an effort to better understand this process, we evaluated biofilm formation, with oral commensal species, by three strains of the subgingivally prevalent microorganism fusobacterium nucleatum and four strains of the periodontopathogen porphyromonas gingivalis. we also tested ...201222771792
subgingival biodiversity in subjects with uncontrolled type-2 diabetes and chronic periodontitis.there is a bidirectional relationship between periodontal disease and type-2 diabetes mellitus (dm). inflammatory mediators may negatively affect glycemic control, and increased glucose levels and resultant glycation end-products may alter the host response against bacterial infection. however, no agreement has been reached regarding the effect of dm on periodontal subgingival microbiota. therefore, the purpose of the present study was to compare the subgingival biodiversity in deep periodontal ...201322762355
antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens.dental plaque accumulation may result in peri-implantitis, an inflammatory process causing loss of supporting bone that may lead to dental implant failure. the antimicrobial activities of six metal and metal oxide nanoparticles and two of their composites against bacterial pathogens associated with peri-implantitis were examined under anaerobic conditions. the activities of nanoparticles of silver (ag), cuprous oxide (cu(2)o), cupric oxide (cuo), zinc oxide (zno), titanium dioxide (tio(2)), tung ...201222727529
subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions.this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ...201222710412
peritonsillar abscess: treatment with immediate tonsillectomy - 10 years of experience.immediate tonsillectomy in patients with peritonsillar abscess is a safe and effective treatment that should be considered as an alternative to conventional incision and drainage.201222708633
a microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard dna-dna hybridization.the aim of this study was to evaluate, by checkerboard dna-dna hybridization, the composition of the microbiota of primary endodontic infections in cases associated with exposed (n = 30) and unexposed (n = 30) pulp space.201222703649
effect of s-prg eluate on biofilm formation and enzyme activity of oral bacteria.recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (s-prg) filler was revealed. we examined the effect of an s-prg eluate on various biologic activities of streptococcus mutans and porphyromonas gingivalis. adherence ability of s. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of p. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. coaggregation of p. gingivalis with ...201222693508
comprehensive analysis of bacterial flora in postoperative maxillary cyst fluid by 16s rrna gene and culture methods.intracystic fluid was aseptically collected from 11 patients with postoperative maxillary cyst (pomc), and dna was extracted from the pomc fluid. bacterial species were identified by sequencing after cloning of approximately 580 bp of the 16s rrna gene. identification of pathogenic bacteria was also performed by culture methods. the phylogenetic identity was determined by sequencing 517-596 bp in each of the 1139 16s rrna gene clones. a total of 1114 clones were classified while the remaining 25 ...201222685668
[lemierre's syndrome as differential diagnosis of lung cancer].lemierre's syndrome is a disseminated infection which is usually caused by fusobacterium necrophorum. an oropharyngeal infection progresses to a septic thrombophlebitis of the internal jugular vein and later metastatic infections throughout the body occur. we present a clinical case in which a patient, initially presenting with symptoms characteristic of pulmonary cancer, turned out to have a rare variant of lemierre's syndrome caused by fusobacterium nucleatum.201222668649
co-localized or randomly distributed? pair cross correlation of in vivo grown subgingival biofilm bacteria quantified by digital image analysis.the polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. a number of these microorganisms have been demonstrated in vitro to interact and enhance biofilm integration, survival or even pathogenic features. to examine the in vivo relevance of these proposed interactions, we extended the spatial arrangement analy ...201222655057
autoinducer 2 of fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens.periodontitis is initiated by bacteria in subgingival biofilms, which are composed mostly of gram-negative anaerobes. autoinducer 2 (ai-2) is a universal quorum sensing (qs) molecule that mediates intergeneric signalling in multispecies bacterial communities and may induce biofilm formation. as fusobacterium nucleatum is the major coaggregation bridge organism that links early colonising commensals and late pathogenic colonisers in dental biofilms via the accretion of periodontopathogens, we hyp ...201322633049
pulmonary botryomycosis on a lung cavity: a rare pulmonary infection mimicking cancer.lung botryomycosis is a rare disease. we report what is to our knowledge the first case occurring on a lung cavity. in a 42-year-old man suffering asthenia and cough, a chest radiograph revealed a right upper lobe opacity. computed tomography scan showed a necrotic mass which was also spiculated. repeated research for mycobacterium tuberculosis was negative. the patient underwent a lobectomy. histological and bacteriological examinations made the diagnosis of botryomycosis, because the cavity pr ...201222627958
the foma porin from fusobacterium nucleatum is a toll-like receptor 2 agonist with immune adjuvant activity.many bacterial components selectively activate immune and nonhematopoietic target cells via toll-like receptor (tlr) signaling; modulation of such host responses defines the immune adjuvant properties of these bacterial products. for example, the outer membrane protein porins from neisseria, salmonella, and shigella are known tlr2 agonists with established systemic and mucosal immune adjuvanticity. early work indicated that the foma porin from fusobacterium nucleatum has immune adjuvant activity ...201222623652
[impact of periodontal disease on arterial pressure in diabetic mice].diabetes-driven cardiovascular diseases represent a high challenge for developed countries. periodontal disease is strictly linked to the aforementioned diseases, due to its gram negative-driven inflammation. thus, we investigated the effects of periodontal disease on arterial pressure during the development of diabetes in mice. to this aim, c57bl/6 female mice were colonized with pathogens of periodontal tissue (porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum) for 1m ...201222621847
microbial diversity in failed endodontic root-filled teeth.persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. this study used 16s rrna sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment.201222613548
occurrence and antimicrobial susceptibility of porphyromonas spp. and fusobacterium spp. in dogs with and without periodontitis.the occurrence of porphyromonas gulae, porphyromonas macacae, fusobacterium nucleatum and fusobacterium canifelinum in subgingival plaque from dogs with and without periodontitis as well as their antimicrobial susceptibility were evaluated. from 50 dogs with periodontitis were identified 38 p. gulae, 8 p. macacae, 26 f. nucleatum and 15 f. canifelinum, and from 50 dogs without periodontitis were identified 15 p. gulae, 12 f. nucleatum and 11 f. canifelinum. all strains were susceptible to most o ...201222609780
neutrophil superoxide production in the presence of cigarette smoke extract, nicotine and cotinine.to determine the effect of cigarette smoke extract, nicotine and cotinine on lucigenin-detectable neutrophil superoxide production.201222607095
the role of coaggregation between porphyromonas gingivalis and fusobacterium nucleatum on the host response to mixed infection.to evaluate the role of coaggregation between porphyromonas gingivalis and fusobacterium nucleatum on the virulence of the mixed infection in mice.201222607053
relationship between oral anaerobic bacteria and otitis media with effusion.in this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (ome), we aimed to investigate the presence and similarity of fusobacterium nucleatum and treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with ome.201222606045
septic arthritis and osteomyelitis in a 10-year-old boy, caused by fusobacterium nucleatum, diagnosed with pcr/16s ribosomal bacterial dna amplification.a 10-year-old boy presented with an atypical non-febrile septic arthritis/osteomyelitis. he was unresponsive to routine antibiotic treatment with flucloxacillin/gentamicin as the pain and fluid collection increased. synovial fluid cultures are negative and gram stain remained negative. only after pcr/16s ribosomal bacterial dna amplification a fusobacterium nucleatum could be detected, and antibiotic therapy switched to clindamycin with rapid response. septic osteomyelitis and arthritis are rela ...201222605875
bacterial pathogens related to chronic suppurative otitis media in individuals with cleft palate: bacteriological culture and polymerase chain reaction.to characterize the microbial etiology of chronic suppurative otitis media comparing the methods of classical bacteriological culture and polymerase chain reaction.201422594401
effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis.emingil g, han b, özdemir g, tervahartiala t, vural c, atilla g, baylas h, sorsa t. the effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. j periodont res 2012; 47: 729-739. © 2012 john wiley & sons a/s background and objective:  to study the effectiveness of azithromycin in combination with nonsurgical periodontal therapy on clinical and microbiological parame ...201222571226
fusobacterium nucleatum and tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model.tannerella forsythia is strongly associated with chronic periodontitis, an inflammatory disease of the tooth-supporting tissues, leading to tooth loss. fusobacterium nucleatum, an opportunistic pathogen, is thought to promote dental plaque formation by serving as a bridge bacterium between early- and late-colonizing species of the oral cavity. previous studies have shown that f. nucleatum species synergize with t. forsythia during biofilm formation and pathogenesis. in the present study, we show ...201222547549
serum antibodies to periodontal pathogens are a risk factor for alzheimer's disease.chronic inflammation in periodontal disease has been suggested as a potential risk factor in alzheimer's disease (ad). the purpose of this study was to examine serum antibody levels to bacteria of periodontal disease in participants who eventually converted to ad compared with the antibody levels in control subjects.201222546352
oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients.oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients.201222531876
quantification of subgingival bacterial pathogens at different stages of periodontal diseases.anaerobic gram-negative oral bacteria such as treponema denticola, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia, campylobacter rectus, and fusobacterium nucleatum are closely associated with periodontal diseases. we measured the relative population (bacterial levels) of these oral pathogens in subgingival tissues of patients at different stages of korean chronic periodontal diseases. we divided the individuals into those with chronic gingivitis (g), moder ...201222526568
fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity.sgp1(t), a strain belonging to a lineage of the phylum synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. for good growth the strain was dependent on co-culture with, or extracts from, selected other oral bacteria. cells of strain sgp1(t) were asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid were produced as end products of metabolism in peptone-yeast extract-glucose broth sup ...201322493171
butyric acid induces apoptosis in both human monocytes and lymphocytes equivalently.short-chain fatty acids (scfas) are metabolites from anaerobic periodontopathic bacteria that induce apoptosis in immune cells such as lymphocytes, monocytes and macrophages. however, it remains unclear if scfas from pathogens induce apoptosis in monocytes/macrophages similarly with lymphocytes. this study investigated whether scfas-induced apoptosis is equal among the immunoregulatory cells. cell apoptosis of the employed human cells was evaluated after treatment with culture supernatants from ...201222466881
mycoplasma pneumoniae preceding lemierre's syndrome due to fusobacterium nucleatum complicated by acute epstein-barr virus (ebv) infectious mononucleosis in an immunocompetent host.we report an unusual case of lemierre's syndrome due to a rare species of fusobacterium, that is, fusobacterium nucleatum preceded by mycoplasma pneumoniae pharyngitis and followed later by epstein-barr virus infectious mononucleosis.201322464641
direct recognition of fusobacterium nucleatum by the nk cell natural cytotoxicity receptor nkp46 aggravates periodontal disease.periodontitis is a common human chronic inflammatory disease that results in the destruction of the tooth attachment apparatus and tooth loss. although infections with periopathogenic bacteria such as porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum) are essential for inducing periodontitis, the nature and magnitude of the disease is determined by the host's immune response. here, we investigate the role played by the nk killer receptor nkp46 (ncr1 in mice), in ...201222457623
adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial.to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients.201222445846
specificity of antimicrobial peptide ll-37 to neutralize periodontopathogenic lipopolysaccharide activity in human oral fibroblasts.the antimicrobial peptide ll-37 is known to have a potent lipopolysaccharide (lps)-neutralizing activity in various cell types. because of observed heterogeneity within periodontopathogenic lps, the authors hypothesized that ll-37 had specificity to neutralize such lps activity. the present study, therefore, aims to investigate the lps-neutralizing activity of ll-37 to various periodontopathogenic lps in interleukin-8 (il-8) production after challenging them in human oral fibroblasts.201322443521
human neutrophil defensins and their effect on epithelial cells.the aims of the present study include: 1) to localize human neutrophil defensin-1 (hnp-1) through hnp-3 in gingiva and in neutrophil extract-treated epithelial cell monolayers; 2) to determine the effects of hnp-1 on the epithelial cell viability, attachment, and spreading; and 3) to analyze the effect of hnp-1 on the bacterial adherence to epithelial cells.201322443519
giant frontal mucocele complicated by subdural empyema: treatment of a rare association.giant frontal mucocele (gfm) is an extremely rare cause of frontal lobe syndrome. subdural empyema (sde) is an uncommon complication of paranasal sinisutis, for which craniotomy and decompressive craniotomy are the most effective surgical procedures. a 54-year-old man was brought unconscious to the emergency room where recurrent generalized seizures occurred. heroine abuse, hcv-related hepatitis, prolonged antibiotic therapy for treatment of purulent rhinorrhea, along with recent personality cha ...201222427297
identification of oral bacterial dna in synovial fluid of patients with arthritis with native and failed prosthetic joints.we examined the presence of bacterial dna in synovial fluids of native or clinically aseptically failed prosthetic joints from patients having periodontal disease and arthritis to determine whether there is bacterial spread from the oral cavity to the joints.201222426587
canine periodontal disease control using a clindamycin hydrochloride gel.stabilizing or reducing periodontal pocket depth can have a positive influence on the retention of teeth in dogs. a topical 2% clindamycin hydrochloride gel (chgel) was evaluated for the treatment of periodontal disease in dogs. the chgel formulation provides for the sustained erosion of the matrix, but also flows into the periodontal pocket as a viscous liquid, and then rapidly forms a gel that has mucoadhesive properties and also may function as a physical barrier to the introduction of bacter ...201122416621
in vitro evaluation of mtad and nisin in combination against common pathogens associated with root canal infection.many pathogenic microorganisms were found in an infected root canal. the object of this study was to evaluate the effect of mtad in combination with nisin on the pathogens associated with root canal infection.201222414835
characterization of microbiota of root canal-treated teeth with posttreatment disease.this study evaluated the microbiota of root canals undergoing retreatment. the most prevalent taxa detected by checkerboard included propionibacterium species, fusobacterium nucleatum, streptococci, and pseudoramibacter alactolyticus. quantitative real-time pcr detected enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. the findings call into question the status of e. faecalis as the main pathogen and sugge ...201222403423
comparative evaluation of the effects of different photoablative laser irradiation protocols on the gingiva of periodontopathic patients.we aimed at quantifying the presence of periodontopathogens in gingival biopsies from periodontitis patients treated with different photoablative lasers (diode gaas, er:yag, nd:yag, and co(2) lasers) and histologically analyzing their effects on the gingiva.201222401128
fusobacterium nucleatum groel induces risk factors of atherosclerosis in human microvascular endothelial cells and apoe(-/-) mice.infection and inflammation are risk factors in the initiation and progression of atherosclerosis. periodontitis is one of the most prevalent chronic inflammations of the oral cavity, and has been reported to be associated with systemic disease. in this study, we evaluated whether the heat-shock protein groel of fusobacterium nucleatum, one of the most prevalent bacteria in periodontitis, induces factors that predispose to atherosclerosis in human microvascular endothelial cells (hmec-1) and apol ...201122394469
anaerobic and microaerophilic pathogens in the biofilm formation on voice prostheses: a pilot study.voice rehabilitation with voice prostheses is a standard therapy in laryngectomized patients. biofilm formation on the surface of the voice prostheses causes device failure and requires frequent replacements. studies analyzing the biofilm of voice prostheses have mainly focused on aerobic bacteria. anaerobic bacteria as an integral part of the biofilms on voice prostheses have not been investigated yet.201222374743
internal decompressive craniectomy with craniotomy: a novel surgical therapy of giant frontal mucocele complicated by subdural empyema.giant frontal mucocele (gfm) is an extremely rare cause of frontal lobe syndrome. subdural empyema (sde) is an uncommon complication of paranasal sinisutis, for which craniotomy and decompressive craniotomy are the most effective surgical procedures.201122368985
effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds.the objective of this paper is to evaluate the effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds (vscs). for this study, 42 subjects were randomly assigned to receive oil samples containing l. salivarius wb21 or a placebo for two weeks. oral assessment and saliva collection were performed on days 1 and 15. bacterial analysis was performed using the real-time polymerase chain reaction and terminal restrictio ...201222368259
[effect of periodontal surgery on the subgingival periodontal microbiota in artificial class iii furcation defects: an experimental study in monkeys].this study was to longitudinally evaluate the change of prevalence of five periodontal putative pathogens in the subgingival plaque of artificial class iii furcation defects at the three time-points, including before the establishment of furcation defects, before and 6 months after periodontal surgery.201222353894
association between oral malodour and periodontal disease-related parameters in the general population.to determine the association between halitosis detection and periodontal status in systemically healthy non-smokers and to assess whether halitosis was related to quantities of fusobacterium nucleatum and porphyromonas gingivalis on the tongue dorsum.201322339235
effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model.oral bacterial biofilms are highly complex microbial communities with up to 700 different bacterial taxa. we report here the use of metatranscriptomic analysis to study patterns of community gene expression in a multispecies biofilm model composed of species found in healthy oral biofilms (actinomyces naeslundii, lactobacillus casei, streptococcus mitis, veillonella parvula, and fusobacterium nucleatum) and the same biofilm plus the periodontopathogens porphyromonas gingivalis and aggregatibacte ...201222328675
molecular analysis of 16s rrna genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars.small subunit rrna sequencing and phylogenetic analysis were used to identify cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites.201222326171
azithromycin as an adjunctive treatment of generalized severe chronic periodontitis: clinical, microbiologic, and biochemical parameters.this study examines the efficacy of azithromycin in combination with non-surgical periodontal therapy on clinical and microbiologic parameters and gingival crevicular fluid (gcf) matrix metalloproteinases-8 (mmp-8) levels over 6 months in patients with severe generalized chronic periodontitis (cp).201222324488
reactivation of latent hiv-1 by a wide variety of butyric acid-producing bacteria.latently infected cells harbor human immunodeficiency virus type 1 (hiv-1) proviral dna copies integrated in heterochromatin, allowing persistence of transcriptionally silent proviruses. it is widely accepted that hypoacetylation of histone proteins by histone deacetylases (hdacs) is involved in maintaining the hiv-1 latency by repressing viral transcription. hiv-1 replication can be induced from latently infected cells by environmental factors, such as inflammation and co-infection with other m ...201222322557
treponema denticola chymotrypsin-like proteinase (ctlp) integrates spirochaetes within oral microbial communities.treponema denticola is found ubiquitously in the human oral cavity and is mainly associated with bacterial communities implicated in the establishment and development of periodontal disease. the ability to become integrated within biofilm communities is crucial to the growth and survival of oral bacteria, and involves inter-bacterial coaggregation, metabolic cooperation, and synergy against host defences. in this article we show that the chymotrypsin-like proteinase (ctlp), found within a high-m ...201222313692
detection of fusobacterium nucleatum in chorionic tissues of high-risk pregnant women.this study was undertaken to investigate the existence of a periodontopathic bacterium, fusobacterium nucleatum, in chorionic tissues of pregnant women, and the effects of f. nucleatum on human chorion-derived cells.201222304677
oral microbial biofilm stimulation of epithelial cell responses.oral bacterial biofilms trigger chronic inflammatory responses in the host that can result in the tissue destructive events of periodontitis. however, the characteristics of the capacity of specific host cell types to respond to these biofilms remain ill-defined. this report describes the use of a novel model of bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. monoinfection ...201222266273
necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study.objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ...201222259811
microbial profile of patients with periodontitis compared with healthy subjects.objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ...201222257880
synergistic effect on biofilm formation between fusobacterium nucleatum and capnocytophaga ochracea.the formation of dental plaque biofilm by specific gram-negative rods and spirochetes plays an important role in the development of periodontal disease. the aim of this study was to characterize biofilm formation by fusobacterium nucleatum and capnocytophaga ochracea. coaggregation between f. nucleatum and capnocytophaga species was determined by visual assay. biofilm formation was assessed by crystal violet staining. enhancement of biofilm formation by f. nucleatum via soluble factor of c. ochr ...201222252100
mucosal invasion by fusobacteria is a common feature of acute appendicitis in germany, russia, and china.to investigate the geographic occurrence of mucosa-invading fusobacteria in acute appendicitis.201522249094
fusobacterium nucleatum biofilm induces epithelial migration in an organotypic model of dento-gingival junction.background: effects of fusobacterium nucleatum biofilm on epithelial cell proliferation, apoptotic cell death and basement membrane constituent collagen iv production were examined in an organotypic dento-epithelial (od-e) model. methods: the od-e model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing tooth pieces on the top. a 3-day-old biofilm of either a laboratory strain (atcc 25586) or a clinical strain (ahn 9508) of f. nucleatum was placed on ...201222248219
cytokine expression in response to root canal infection in gnotobiotic mice.to examine cytokine expression profiles during periapical lesion development in response to synergetic human pathogens in a gnotobiotic mouse model.201222233143
release of metronidazole from electrospun poly(l-lactide-co-d/l-lactide) fibers for local periodontitis treatment.we aimed to achieve detailed biomaterials characterization of a drug delivery system for local periodontitis treatment based on electrospun metronidazole-loaded resorbable polylactide (pla) fibers.201222226009
subgingival microbiota in adult down syndrome periodontitis.the subgingival microbiota in down syndrome and non-down syndrome adults receiving periodic dental care was examined for 40 bacterial species using checkerboard dna-dna hybridization and the results were related to clinical periodontal attachment loss.201222221039
investigation and quantification of key periodontal pathogens in patients with type 2 diabetes.diabetes is a recognized risk factor for periodontitis. there are conflicting data regarding whether healthy diabetic patients or diabetic patients with chronic periodontitis have an altered subgingival microbiota compared with nondiabetic individuals. the aim of the present study was to detect quantitative differences in selected periodontopathogens in the subgingival plaque of diabetic patients using taqman quantitative pcr.201222220967
adhesion of aggregatibacter actinomycetemcomitans and fusobacterium nucleatum on bioactive tio(2) surfaces.bioactive tio(2) coatings have been found to enhance fibroblast adhesion and gingival attachment on the titanium surfaces, but no information is available whether the coatings also promote the adhesion of periodontal pathogens.201322220677
in vitro antibacterial activity of galls of quercus infectoria olivier against oral pathogens.the galls of quercus infectoria are commonly used in malay traditional medicine to treat wound infections after childbirth. in india, they are employed traditionally as dental applications such as that in treatment of toothache and gingivitis. the aim of the present study was to evaluate the antibacterial activity of galls of quercus infectoria olivier against oral bacteria which are known to cause dental caries and periodontitis. methanol and acetone extracts were screened against two gram-posi ...201222203875
adherence to streptococci facilitates fusobacterium nucleatum integration into an oral microbial community.the development of multispecies oral microbial communities involves complex intra- and interspecies interactions at various levels. the ability to adhere to the resident bacteria or the biofilm matrix and overcome community resistance are among the key factors that determine whether a bacterium can integrate into a community. fusobacterium nucleatum is a prevalent gram-negative oral bacterial species that is able to adhere to a variety of oral microbes and has been implicated in playing an impor ...201122202886
a five-species transcriptome array for oral mixed-biofilm studies.oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array designs.201122194794
automated rna structure prediction uncovers a kink-turn linker in double glycine riboswitches.the tertiary structures of functional rna molecules remain difficult to decipher. a new generation of automated rna structure prediction methods may help address these challenges but have not yet been experimentally validated. here we apply four prediction tools to a class of double glycine riboswitches that can bind two ligands cooperatively. a novel method (bppalign), rmdetect, jar3d, and rosetta 3d modeling give consistent predictions for a new stem p0 and a kink-turn motif. these elements st ...201122192063
Microbiological analysis of gingivitis in pediatric patients under orthodontic treatment.The aim of this study was to determine the relationship between gingival inflammation and changes in bacteria of the gingival sulcus in children in orthodontic treatment with brackets.201122185242
Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis.OBJECTIVE: The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR). MATERIALS AND METHODS: Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomit ...201122169888
Oral Bacterial DNA Differ in Their Ability to Induce Inflammatory Responses in Human Monocytic Cell Lines.BACKGROUND: DNA of periodontal pathogens, Porphyromonas gingivalis (Pg-DNA) and Tannerella forsythia, stimulate cytokine production in human monocytic cells (THP-1) through Toll like receptor 9 (TLR-9) and nuclear factor kappa B signaling. Fusobacterium nucleatum is one of the most frequently isolated bacteria in periodontally-diseased tissues and reported to synergize with Porhyromonas gingivalis enhancing the pathogenicity. We investigated inflammatory mediator production in THP-1 cells challe ...201122166166
Antibacterial activity of sphingoid bases and fatty acids against gram-positive bacteria and gram-negative bacteria.There is growing evidence that the role of lipids in innate immunity is more important than previously realized. How lipids interact with bacteria to achieve a level of protection, however, is still poorly understood. To begin to address mechanisms of antibacterial activity, we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of lipids common to the skin and oral cavity: sphingoid bases D-sphingosine, phytosphingosine, and dihydrosphingosine, and f ...201122155833
The adhesion of oral bacteria to modified titanium surfaces: role of plasma proteins and electrostatic forces.OBJECTIVES: Modifications of titanium (Ti) implant surfaces have a significant effect on early biofilm formation and the outcome of implant procedures. The aim of this study was to examine the role of plasma proteins and electrostatic forces in the adhesion mechanism of oral bacteria to modified Ti surfaces. MATERIALS AND METHODS: Ti discs with three different types of surface modifications, machined, acid-etched, and acid-etched and blasted, were examined for adhesion of oral bacteria: Streptoc ...201122150723
extracellular deoxyribonuclease production by periodontal bacteria.palmer lj, chapple ilc, wright hj, roberts a, cooper pr. extracellular deoxyribonuclease production by periodontal bacteria. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01451.x © 2011 john wiley & sons a/s background and objective:  whilst certain bacteria have long been known to secrete extracellular deoxyribonuclease (dnase), the purpose in microbial physiology was unclear. recently, however, this enzyme has been demonstrated to confer enhanced virulence, enabling bacteria to evade the ...201122150619
Signal peptide of FadA adhesin from Fusobacterium nucleatum plays a novel structural role by modulating the filament's length and width.FadA, a novel adhesin of periodontal pathogen Fusobacterium nucleatum is composed of two forms, pre-FadA and mature FadA (mFadA), constituting the functional FadA complex (FadAc). By electron microscopy, we observed that mFadA formed uniformly long and thin filaments, while FadAc formed heterogeneous filaments of varying lengths and widths, as well as "knots". Mutants in signal peptide or in the non-alpha-helical loop retaining heterogeneous structures had binding activity while those forming ag ...201222108653
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