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[unwanted modification of the thyroid gland by drugs with special reference to nonsteroidal antirheumatic agents].in two retrospective clinical studies was investigated the influence of the modern non-steroidal antirheumatic drugs indometacin and diclofenac (rewodina) on the thyroid gland and corresponding peripheral hormone parameters. under longterm treatment with indometacin a moderate strumigenic effect could be observed, which could not clearly be proved under the diclofenac therapy. in all patients with rheumatoid arthritis, independent of the kind of pharmacotherapy, decreased t3-hormone levels were ...19863087075
influence of glucocorticoids on calcium absorption in different segments of the rat intestine.glucocorticoids enhance the movement of fluid and sodium in the duodenum, thereby resulting in an increase in the passive transport of calcium. since passive transport of calcium predominates in the distal intestinal segments, the influence of glucocorticoids on calcium and fluid transport in the duodenum, mid-jejunum, ileum, and colon of the rat was studied. calcium transport and fluid absorption was determined by the in vivo ligated loop technique. one segment was ligated in each animal. under ...19863087601
hypophysectomy does not alter the increase in striatal dopamine receptor density produced by 6-hydroxydopamine lesions in male rats.while hypophysectomy can alter the increase in striatal dopamine (da) receptor density observed after estrogen, haloperidol or prolactin treatments, it does not alter the increase in density found after 6-hydroxydopamine lesions of the da neurons in male rats. this observation suggests that striatal da receptors can be regulated by at least two distinct mechanisms, only one of which requires the availability of pituitary hormones.19863092152
emergence of beta-lactamase producing anaerobic bacteria in the tonsils during penicillin treatment.the emergence of beta-lactamase producing bacteria in the microflora in the oropharyngeal cavity was studied in ten healthy volunteers treated with 1 g phenoxymethylpenicillin b.i.d. for ten days. beta-lactamase activity in saliva was also investigated. a significant increase in the number of beta-lactamase producing strains of bacteroides species and fusobacterium nucleatum was observed. one beta-lactamase producing staphylococcus aureus strain was recovered in one of the volunteers before the ...19863093222
on the mechanism of antithrombotic action of flavonoids.flavonols (quercetin and rutin) and flavanes (cyanidol and meciadonol) were studied for their effect on non-enzymatic lipid peroxidation, lipoxygenase and cyclo-oxygenase activities, binding to albumin and platelet membranes. these biochemical properties of four flavonoids were compared with respect to their antithrombotic action in vivo and their efficacy at influencing the platelet-endothelium interaction in vitro. all four flavonoids inhibited the ascorbate-stimulated formation of malondialde ...19873101704
comparison of the chinese schema and the international antigenic typing system for serotyping pseudomonas aeruginosa.twelve strains of pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the people's republic of china were compared with serogroups in the international antigenic typing system (iats). the first eight groups originated in the people's republic of china, and group ii appears to have a new major antigen that is not found in the iats. groups i, iii, iv, v, vi, vii, and viii correspond to groups 11, 6, 9, 4, 8, 3, and 1, respectively, of the iats. groups ix, x, xi, an ...19873108310
cloning of reticulocyte lipoxygenase mrna. 19873109402
amino acid-dependent transport of sugars by fusobacterium nucleatum atcc 10953.resting cells of fusobacterium nucleatum 10953 (grown previously in a medium containing glucose) failed to accumulate glucose under aerobic or anaerobic conditions. however, the addition of glutamic acid, lysine, or histidine to anaerobic suspensions of cells caused the immediate and rapid accumulation of glucose. except for the amino acid-dependent transport of galactose and fructose (the latter being transported at approximately one-third the rate of glucose), no other sugars tested were accum ...19873114229
lysozyme-protease-inorganic monovalent anion lysis of oral bacterial strains in buffers and stimulated whole saliva.similar to streptococcus mutans, buffer suspensions of lactobacillus casei, lactobacillus plantarum, and fusobacterium nucleatum all undergo cell lysis when treated with the lysozyme-protease-inorganic monovalent anion antibacterial system. for lactobacillus species treated with lysozyme and proteases at phs of 4 and 5.3, lysis resulted when a lytic activating concentration of bicarbonate anion followed enzyme treatment. furthermore, synergistic lysis of these bacteria was noted when lysozyme-pr ...19873114343
schistosoma mansoni polypeptides immunogenic in mice vaccinated with radiation-attenuated cercariae.we compared the humoral immune response of mice protected against schistosoma mansoni by vaccination with radiation-attenuated cercariae to that of patently infected mice, and we identified antigens that elicit a greater, or unique, immune response in the vaccinated mice. these comparisons were based upon radioimmunoprecipitations and immunodepletion of [35s]methionine-labeled schistosomular and adult worm polypeptides, followed by one- and two-dimensional polyacrylamide gel analyses. the humora ...19873116082
the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid.the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ...19873117781
evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2.the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ...19873121647
lactate dehydrogenase and malate dehydrogenase of sertoli cells in rats.lactate-dehydrogenase (ldh) and malate-dehydrogenase (mdh) activities and isozyme patterns of maturing sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. both enzymes, and more strikingly ldh, increased between 2 and 3 weeks of age. ldh isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. these data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of ...19873122678
identification of biological false positives in anti-hiv antibody tests. 19873122793
characterization of the ksga gene of escherichia coli determining kasugamycin sensitivity.in the plasmid puc8ksga7, the coding region of the ksga gene is preceded by the lac promoter (plac) and a small open reading frame (orf). this orf of 15 codons is composed of nucleotides derived from the lacz gene, a multiple cloning site and the ksga gene itself. the reading frame begins with the atg initiation codon of lacz and ends a few nucleotides beyond the atg start codon of ksga. the ksga gene is not preceded by a shine-dalgarno (sd) signal. cells transformed with puc8ksga7 produce activ ...19873122846
[effect of eicosanoids on the accumulation of cholesterol and the proliferation of subendothelial cells in the human aorta].the effect of prostacyclin and stable thromboxane analog a2 on endothelial culture of human aorta was studied. it was shown that prostacyclin inhibited accumulation of cholesterol in the cells and their proliferation, while thromboxane exhibited an opposite effect. calcium antagonists potentiated effects of prostacyclin and inhibited them in respect to thromboxane. screening of a number of synthetic agents affecting arachidonic acid metabolism was carried out. it was found that lipoxygenase inhi ...19883122858
cross-reactivity among legionella species and serogroups.cross-reactions of 17 members of the family legionellaceae were studied by four different serological techniques: immunofluorescence (if), slide agglutination (sa), microagglutination (ma) and immunodiffusion (id), using antigens and rabbit antisera prepared in our laboratory. results obtained corresponded closely with those described by other authors, especially for if and sa. the 17 antigens were further tested by if with a panel of sera previously diagnosed as positive for legionella. a high ...19873123264
effect of aldose reductase inhibition on nerve conduction velocity and resistance to ischemic conduction block in experimental diabetes.the relationships between nerve polyol levels and both nerve conduction velocity (ncv) and resistance to ischemic conduction block (ricb) in streptozocin-induced diabetic rats were examined in two studies. in the first study, sciatic ncv and ricb of the tail nerve, assessed by measuring the time to disappearance of the nerve action potential after the tail was rendered ischemic, were measured in nondiabetic rats, untreated diabetic rats, and diabetic rats given statil, an aldose reductase inhibi ...19883133261
synthetic aci-reductones: 3,4-dihydroxy-2h-1-benzopyran-2-ones and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers. antiaggregatory, antilipidemic, and redox properties compared to those of the 4-substituted 2-hydroxytetronic acids.synthetic procedures for the elaboration of aci-reductones belonging to the 6- or 7-mono- or bis-substituted-3,4-dihydroxy-2h-1-benzopyran-2-ones (6-10) and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers (11, 12) are described. hexahydrobenzopyranone aci-reductones were conveniently prepared by using meldrum's synthon (2,2-dimethyl-1,3-dioxane-4,6-dione, 49). certain of these substances were evaluated for antilipidemic activity in the cholesterol-fed rat model, and all analogues were ...19883133477
the effect of nicotine on human endothelial cell release of prostaglandins and ultrastructure.the acute and sub-acute effects of nicotine at concentrations between 10(-9)m and 10(-2)m on human umbilical vein endothelial cell release of prostacyclin and prostaglandin e2 and the sub-acute effect on the endothelial cell ultrastructure have been examined. the acute effect of nicotine on prostaglandin release has been assessed by measuring release of prostacyclin and prostaglandin e2 following stimulation of confluent monolayers of endothelial cells with a23187 in the presence of nicotine. th ...19883134041
influence of radiant heat stress on respiratory water loss in new-born lambs.respiratory water loss, oxygen consumption and carbon dioxide production were measured during rest and during moderate radiant heat stress in 9 healthy unanaesthetized lambs, age 4-15 days. the method used is based on an open flow-through system with a mass spectrometer for gas analyses. respiratory water loss was 12.7 mg/kg min when the lamb was at rest and increased to 24.7 mg/kg min during heat stress. oxygen consumption was 13.9 ml/kg min at rest and remained unchanged during heat stress. th ...19883134055
analysis of phorbol ester stimulated human megakaryocyte development.megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...19883134065
analysis of phorbol ester stimulated human megakaryocyte development.megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...19883134065
comparative studies on the low density lipoprotein subfractions from pig and man.(1) by means of a single spin density gradient ultracentrifugation method, sera from six human volunteers and six pigs were studied; three or four distinct low density lipoprotein (ldl) subfractions could be seen in the pig serum (ldl-1a, ldl-1, ldl-2 and ldl-3) and three in the human serum (ldl-1a, ldl-1b and ldl-2). the subdivision of ldl into subfractions was less clear for the human subjects than for the pigs. (2) physicochemical characteristics (chemical composition, apoprotein content, siz ...19883136968
[results of growth hormone treatment in slow-growing children with normal plasma growth hormone levels in provocation tests]. 19883137696
enzymatic capacity and protein synthesis in human muscle postoperatively.the glycolytic and aerobic oxidative capacity in skeletal muscle was investigated to reveal if the decrease seen in muscle protein synthesis is accompanied by a fall in the enzymatic capacity to oxidize substrates. six patients undergoing elective abdominal surgery were investigated by percutaneous muscle biopsies taken before surgery and on the first and third postoperative days. protein synthesis as assessed by the polyribosome concentration was 40% lower on the third day postoperatively than ...19883144105
specific responses to staphylococcus aureus in patients with hyper-ige syndrome. 19873144122
a new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene.we have constructed a new expression vector for mammalian cells. the vector contains a truncated tk gene for amplification under selective conditions, a sequence putatively supporting the replication of plasmid dna in eukaryotic cells (murine autonomously replicating sequence) and an expression cassette for the cdna to be studied. as a model cdna we have used that of human tissue-type plasminogen activator (t-pa). analysis of hirt supernatants and chromosomal dna from l cells, prepared six weeks ...19883149608
microbe-induced lymphocyte blastogenesis enhancement after preculture.the in vitro blastogenic response of human peripheral blood mononuclear cells to fusobacterium nucleatum and other oral microorganisms was enhanced if the peripheral blood mononuclear cells were cultured for 24 h at 37 degrees c prior to the addition of stimulant. the enhancement which occurred at optimal and supraoptimal concentrations of f. nucleatum (10 to 100 micrograms/ml) was detected after a preculture period of as little as 2 h. the blastogenic response was a result of t-cell proliferati ...19853156816
human and bovine endothelial cells synthesize membrane proteins similar to human platelet glycoproteins iib and iiia.human umbilical vein endothelial (huve) and bovine aortic endothelial (bae) cells in culture were examined to determine whether membrane proteins similar to human platelet glycoproteins (gp) iib and iiia were present. the huve and bae cells were either 125i-surface labeled or metabolically labeled. triton x-100 lysates of labeled cells were immunoprecipitated with polyclonal antibodies prepared against purified human platelet gp iib-iiia complex. two membrane proteins were detected on both huve ...19853161885
refractory periodontitis: mixed infection with bacteroides gingivalis and other unusual bacteroides species. a case report.host immune response and the predominant subgingival microflora were evaluated in a 47-year-old male exhibiting severe, recurrent periodontitis. the patient's neutrophils were chemotactically elevated but other functions were within normal limits. significantly, bacteroides gingivalis and bacteroides zoogleoformans constituted 80% of the cultivable microflora and total cell count in subgingival plaque. the remainder of the cultivable microbiota was comprised of fusobacterium nucleatum and haemop ...19883162983
[primary myelofibrosis showing megakaryoblastic crisis: a case report]. 19883164424
[in vitro organ cultures of human embryonic and fetal thymus]. 19883168902
characteristics of glutamate dehydrogenase, a new diagnostic marker for the genus fusobacterium.enzymes representative of carbohydrate and nitrogen metabolism were screened for their presence and activity amongst species of the genus fusobacterium. glutamate dehydrogenase (gdh) was reliably detected in all 25 strains studied. the ph profile of this enzyme and the dna base composition of selected strains were also determined. dna base composition of selected strains ranged between 28-32.9 mol% g + c. gdh was active between ph 7.5-11.5 but two ph profiles of activity, with optima at 9.5 and ...19883171543
effect of mycoplasmas on natural cytotoxic activity and release of tumor necrosis factor alpha by spleen cells.it has been reported that mycoplasma-infected cells are more sensitive to lysis by natural cytotoxic (nc) effector cells and that splenic nc cells release tumor necrosis factor (tnf-alpha) when they lyse sensitive target cells. here we showed that spleen cells released tnf-alpha when they were incubated with nc-sensitive cells that were infected with mycoplasmas or when they were incubated with mycoplasmas alone, but did not release tnf-alpha when incubated with nc-sensitive cells that were not ...19883182071
tetracycline resistance and tetm in oral anaerobic bacteria and neisseria perflava-n. sicca.tetracycline-resistant organisms isolated from six patients with periodontal disease included bacteroides spp., eubacterium spp., fusobacterium nucleatum, neisseria perflava-n. sicca, peptostreptococcus anaerobius, veillonella parvula, and facultative streptococci. all but the bacteroides spp. and eubacterium spp. hybridized with the tetm determinant. an additional 417 bacterial strains were screened, and 4% of both the oral streptococci and the fusobacterium spp. hybridized with the tetm probe.19883190205
[analysis of the hypothermic action of etimizol].in chronic experiments on rabbits it was shown that the hypothermic effect appears in the series of antipheins after the addition of ethyl radical in the first position of imidazole ring. the hypothermic effect of etimizol is more pronounced in the brain than in the body, whereas caffeine equally increases the temperature of the brain and body.19883191969
[comparative study of the progestagenic action of 16 alpha, 17 alpha-cyclohexanoprogesterones (pregna-d'6-pentarans) when administered by different routes].a comparative study of the progestational activity of modified pentarans depending on the dose and the route of administration was carried out. it was shown that modification of the molecule of 16 alpha, 17 alpha-cyclohexanoprogesterones by introducing and additional double bond in positions 6, 7 and methyl group in position 6 provided a high gestational effect, being particularly pronounced at intramuscular administration.19883191979
cauda equina syndrome secondary to disseminated zygomycosis.we describe a case of cauda equina syndrome secondary to disseminated zygomycosis. a 52-year-old man had myelodysplastic syndrome, progressive weakness of the lower extremities, and incontinence. neuroradiological findings were consistent with lumbar stenosis and probable disk herniation. a lung lesion was also discovered on the chest roentgenogram. the patient's condition rapidly deteriorated after surgical decompression. attempts at determining other etiologies were unsuccessful. at autopsy, d ...19883193592
functional and structural alterations of the nervous system induced by avian retrovirus rav-7.chickens infected as embryos with rav-7 developed neurological signs including ataxia, lethargy, and imbalance. evoked spinal cord potentials for rav-7 infected sc chickens were considerably slower (64.8 m/s) than for uninfected sc (103.4 m/s), genetically hypothyroid (os) (93.9 m/s) or special c (95.1 m/s) chickens. conduction velocity measurements of sciatic nerves showed normal values for all the chickens examined in this study. histopathological studies revealed non-suppurative meningoenceph ...19883193874
functional and structural alterations of the nervous system induced by avian retrovirus rav-7.chickens infected as embryos with rav-7 developed neurological signs including ataxia, lethargy, and imbalance. evoked spinal cord potentials for rav-7 infected sc chickens were considerably slower (64.8 m/s) than for uninfected sc (103.4 m/s), genetically hypothyroid (os) (93.9 m/s) or special c (95.1 m/s) chickens. conduction velocity measurements of sciatic nerves showed normal values for all the chickens examined in this study. histopathological studies revealed non-suppurative meningoenceph ...19883193874
the comparative cytotoxicity of periodontal bacteria.the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ...19883204481
evolution of influenza polymerase: nucleotide sequence of the pb2 gene of a/chile/1/83 (h1 n1).the complete nucleotide sequence of the pb2 gene of influenza virus a/chile/1/83 (h1 n1) is presented. sequence comparison between a/chile pb2 protein and the known pb2 sequences of the influenza strains a/wsn/33 (h1 n1), a/pr/8/34 (h1 n1), a/nt/60/68 (h3 n2), a/kiev/59/79 (h1 n1), a/fpv/rostock/34 (h7 n1), and b/ann arbor/1/66 indicates extensive amino acid homology for the influenza a virus pb2 proteins. small clusters of basic amino acids are conserved in all pb2 proteins including the influe ...19883214273
outer membrane protein pattern of eubacterium plautii.the outer membrane sds-page pattern of eubacterium plautii was characterized by a large number of surface exposed low- and high-molecular-mass proteins. silver stainable carbohydrate was not present. the pattern was clearly distinct from those of outer membrane preparations of eubacterium saburreum and fusobacterium nucleatum. the results are compatible with a gram-positive cell wall structure in e. plautii.19883221197
[nursing research based on human engineering: the home environment]. 19883221700
in vitro activity of pefloxacin compared with five other quinolones on nalidixic acid-resistant proteae species.the in vitro activity of pefloxacin, a new fluoroquinolone, was compared with that of 5 other quinolone compounds (nalidixic and pipemidic acids, norfloxacin, ciprofloxacin, and ofloxacin) against 416 strains of proteae spp. isolated from urine specimens of hospitalized patients with acute urinary tract infections (uti). ciprofloxacin was the most active agent. norfloxacin, ofloxacin, and pefloxacin were similarly active against proteus strains (mic90 = 0.39 microgram/ml). against providencia sp ...19883224396
a combined tension band and lag screw technique for fixation of olecranon osteotomies.a combined tension band and lag screw technique for fixation of olecranon osteotomies was used in six canine clinical patients weighing 4.5 to 19 kg. after the proximal part of the ulnar shaft was exposed, a screw hole was drilled and tapped just cranial to the caudal cortex of the olecranon. an osteotomy was performed and the hole in the olecranon fragment was overdrilled to form a gliding hole. for reconstruction, the olecranon was reduced anatomically and compressed with a screw placed in lag ...19883232327
a combined tension band and lag screw technique for fixation of olecranon osteotomies.a combined tension band and lag screw technique for fixation of olecranon osteotomies was used in six canine clinical patients weighing 4.5 to 19 kg. after the proximal part of the ulnar shaft was exposed, a screw hole was drilled and tapped just cranial to the caudal cortex of the olecranon. an osteotomy was performed and the hole in the olecranon fragment was overdrilled to form a gliding hole. for reconstruction, the olecranon was reduced anatomically and compressed with a screw placed in lag ...19883232327
effects of progressively increased doses of theophylline and of s 9795 on hemodynamics, blood gases and lung mechanics in dogs.we investigated the effects of progressively increased i.v. doses of theophylline and of the newly introduced methylxanthine derivative s 9795 on hemodynamics, blood gases and lung mechanics in 2 groups each of 8 pentobarbital sodium-anesthetized dogs. the maximum plasma levels that were attained were 56.6 +/- 3.4 micrograms/ml (mean +/- s.e.) for theophylline and 12 +/- 1.2 micrograms/ml for s 9795. theophylline increased heart rate and cardiac output, decreased arterial po2, increased o2 consu ...19883233049
lack of correlation between anatomic infarct size and ventriculographic variables following coronary occlusion in closed-chest dogs.1. although the effects of therapeutic interventions upon infarct size are frequently assessed on the basis of left ventricular ejection fraction and segmental contraction, the correlation of these variables with infarct size has not been thoroughly evaluated. to explore such relationships, we occluded the left anterior descending coronary artery of 22 closed-chest dogs and performed contrast ventriculography one week later. regional myocardial function was evaluated by a computer system in 60 r ...19883248241
[acid soluble glycoprotein in human saliva with monoclonal antibody]. 19883253589
ph changes during simultaneous metabolism of urea and carbohydrate by human salivary bacteria in vitro.the effect of the wide natural variation in oral ureolysis rates on the ph changes resulting from simultaneous metabolism of 25 mm urea and 2.8 mm glucose in salivary-sediment bacteria were investigated. the ph curves were complex, and included distinctive plateaux indicative of balanced acid and base production. these neutralization plateaux occurred at different phs, which were a function (r2 = 0.98) of the ureolytic rate as measured by the log of the initial ph-change rate in the urea-only re ...19883254127
evidence for the existence of two classes of corncob (coaggregation) receptor in fusobacterium nucleatum. 19883254470
production of mouse monoclonal antibodies for the analysis of idiotypes in serum of patients with chronic lymphatic leukaemia.in this report a simple procedure for the production of murine monoclonal antibodies (moab) against the idiotype of malignant b cells is described. mice were immunized with lymphoid cells from patients with b-cell chronic lymphocytic leukaemia (b-cll). after fusion of the spleen cells, hybridoma supernatants were screened for anti-idiotypic moab in elisa with immunoglobulins obtained from tumour-cell lysates, xenohybridomas and patients' sera. the anti-idiotypic moab were used to study tumour ce ...19883257881
the function of human intercellular adhesion molecule-1 (icam-1) in the generation of an immune response.monoclonal antibody rr 1/1 directed against the putative lfa-1 ligand molecule intracellular adhesion molecule-1 (icam-1) was found to inhibit the t cell proliferative response to the antigen ppd. interestingly, the percentage of unstimulated monocytes which expressed icam-1 on their surface appeared to vary greatly from person to person although the majority of monocytes did express high levels of icam-1 within their cytoplasm and surface expression could be rapidly induced on most cells by adh ...19883257922
antibody synthesis specific for nonoral antigens in inflamed gingiva.in vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal b-cell activators (pba). we reasoned that if pba were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate. to test this, rabbits with experimental periodontitis were immunized in the hind legs with the histochemically detectable antigen horseradish peroxidase (hrp) or glucose oxidase ( ...19883257940
alloimmune b-lymphocytes modify the myocardium contractility-inducing release of srs-a.it has been previously demonstrated that murine alloimmune lymphoid cells were able to exert positive or negative inotropic effects on isolated mouse atria depending on the cellular type used. here we show that balb/c anti-c3h b-cells induced positive inotropic action on c3h mouse atria. supernatants of alloimmunized b-cells co-cultivated with c3h myocardium exerted the same biological effect as alloimmune b-cells, indicating that a soluble factor is involved. inhibitors of lipoxygenase(s) of ar ...19883263338
a second chain of human cd8 is expressed on peripheral blood lymphocytes.human cd8 has been thought to consist of disulfide-linked homodimers and homomultimers of a single polypeptide chain homologous to mouse and rat cd8 alpha. in contrast, mouse and rat cd8 are composed of disulfide-linked heterodimers of alpha and beta chains. we have now isolated and sequenced cdna clones encoding a human homologue of mouse and rat cd8 beta. one such clone was inserted into an expression vector and its encoded product was shown to be expressed on the cell surface after cotransfec ...19883264320
expression of a functional cd3-ti antigen/mhc receptor in the absence of surface cd2. analysis with clonal jurkat cell mutants.to investigate the requirement for cd2 expression in activation of t lymphocytes via the cd3-ti antigen/mhc receptor complex, we produced and characterized a series of cd2- jurkat variants. these mutants lack detectable surface cd2 as determined by indirect immunofluorescence, immunoprecipitation analysis, and specific radiolabeled antibody binding assay, but nevertheless, expressed normal numbers of cd3-ti receptors. as expected, the combination of anti-cd2 antibodies, termed anti-t112 and anti ...19883264323
immunobiological properties of lipopolysaccharides isolated from fusobacterium nucleatum and f. necrophorum.lipopolysaccharides (lpss) were isolated from fusobacterium nucleatum atcc 10953 and f. necrophorum atcc 25286 by the hot phenol/water procedure. f. nucleatum lps was composed of 16% (w/w) carbohydrate, 10% (w/w) hexosamine and 40% (w/w) fatty acid, while f. necrophorum lps was composed of 26% (w/w) carbohydrate, 12% (w/w) hexosamine and 28% (w/w) fatty acid. these lps preparations induced mitogenic responses in spleen cells of balb/c, balb/c (nu/nu) and c3h/hen mice, and these responses were su ...19883265430
oscillations and chaos in epidemics: a nonlinear dynamic study of six childhood diseases in copenhagen, denmark.using traditional spectral analysis and recently developed non-linear methods, we analyze the incidence of six childhood diseases in copenhagen, denmark. in three cases, measles, mumps, rubella, the dynamics suggest low dimensional chaos. outbreaks of chicken pox, on the other hand, conform to an annual cycle with noise superimposed. the remaining diseases, pertussis and scarlet fever, remain problematic. the real epidemics are compared with the output of a monte carlo analog of the seir model f ...19883266037
virus-specific memory t cells are pgp-1+ and can be selectively activated with phorbol ester and calcium ionophore.memory lymphocytic choriomeningitis virus (lcmv)-immune cytotoxic t-lymphocyte precursors (ctlp) can be stimulated to proliferate and to mediate specific cytotoxic activity following incubation with phorbol myristate acetate (pma), calcium ionophore (cai), and interleukin 2 (il-2). this protocol can be used to selectively induced virus-specific ctl activity under both bulk culture and limiting dilution conditions, in the absence of added antigen. there is no concurrent stimulation of alloreactiv ...19883282677
on the prevention and treatment of calcification disorders of old age.from several investigations it is known that magnesium oxide or hydroxide therapy causes a considerable delay of kidney stone recurrence in man. further, it is known that the mortality of populations due to cardiovascular disease in areas with hard drinking water containing more magnesium and having a higher mg/ca ratio than soft water, is considerably lower than in soft water areas. in the present study a physiological model for the homeostasis of calcium and phosphate is given. it is shown tha ...19883285142
inhibitory effect of gnrh on isolated rat uterine muscle contractility.the effect of gnrh upon uterine contractions of both non-pregnant and pregnant rats was examined in vitro. in the non-pregnant rat uterus, gnrh inhibited in a concentration-and-time dependent manner the contractions induced by acetylcholine and oxytocin, but not those caused by bradykinin and angiotensin ii. gnrh also inhibited the rhythmic contractions induced by oxytocin in uterine strips from late pregnant rats. these findings show that gnrh has a direct inhibitory effect on the rat uterine c ...19883290605
neural mechanisms of binocular vision. 19863303676
the growth of oral bacteria on saliva.the present experiments were aimed at studying the degradation of salivary glycoproteins by the oral microflora. to this end, s. sanguis i strain ny476 and s. sanguis ii (s. mitior) strain ny581 were grown continuously in human-whole saliva. under these conditions, the strains produced a variety of cell-associated hydrolytic activities, including glycosidases, exo- and endopeptidases, and esterases. s. sanguis ii generally exhibited higher levels of enzyme activity than did s. sanguis i, in part ...19873305628
t-lymphocyte and experimental autoimmune uveoretinitis.the immunopathogenic mechanisms underlying s-antigen-induced experimental autoimmune uveoretinitis (eau) were discussed with particular emphasis on the cellular immune response on the basis of the following data: 1) athymic nude rats did not develop eau unless sensitized lymphocytes obtained from heterozygous rats were transferred into the nude rats; 2) cyclosporine, an immunosuppressant selective to the t-lymphocytes, completely inhibited the cellular immune response to s-antigen as well as eau ...19873312733
bacterial adherence on replicas of sodium dodecyl sulfate-polyacrylamide gels.a method for determining which molecules in a complex mixture of proteins can function as bacterial receptors was devised. salivary proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose. bacteria that were metabolically labeled with 3h or externally labeled with 125i were incubated on the nitrocellulose replicas. after 18 h at 4 degrees c, the unbound cells were removed by repeated washing of the replicas, and the bands to which th ...19873314581
[effect of single 60co whole-body irradiation on the absolute count and degree-of-maturity distribution of reticulocytes in the rat].wistar-rats were exposed to a single 60co-gamma-total body irradiation with 1.5 gy, 3.0 gy and 4.5 gy. the trend of reticulocyte count and the reticulocyte maturation distribution were measured with an automatic analysis system over a period of 30 days. in all animals the reticulocyte count as well as the maturation distribution showed minima at the second and the twelfth day post irradiation. the duration of the minima were dose dependent. the changes of the reticulocyte count and the maturatio ...19873317952
micro in vitro test for chloroquine resistance of plasmodium falciparum: discrepancies attributable to batch variation of plates. 19873318026
abo blood groups and escherichia coli sepsis. 19873318030
pulsatile secretion of growth hormone and insulin in relation to feeding in rats.in unrestrained male wistar rats chronically implanted with intracardiac catheters, blood samples were taken every 20 min throughout the 24 h of the diurnal cycle. plasma concentrations of growth hormone (gh), insulin, and glucose were measured. the pattern of food intake was continuously monitored. the existence of 3-h pulsatile cycles of gh secretion was confirmed. in addition, short bursts of insulin secretion were observed in the middle of every second gh peak-to-peak interval. food intake a ...19873318507
[medico-genetic studies of human provisional organs]. 19873318529
biologically active luteinizing hormone (lh) in klinefelter's syndrome: response to gonadotropin-releasing hormone (gnrh) and effects of testosterone undecanoate.basal and gonadotropin-releasing hormone (gnrh)-stimulated levels of biologically active and immunoreactive lh (blh and ilh) were measured in six patients with klinefelter's syndrome (ks) (mean age 24.7 years). in the same patients the diurnal rhythm of serum testosterone (t) was investigated (morning values vs. evening values). the results were compared with those obtained in ten normal young men (mean age 29.3 years). moreover, in one patient with ks we studied the effects of testosterone unde ...19873318557
anti-candida drugs--the biochemical basis for their activity.the past years have seen a continuous effort toward the synthesis of new antifungal agents. most of them belong to the n-substituted imidazoles and triazoles. another interesting series of antifungals are the allylamines. biochemically, both the azole derivatives and the allylamines belong to the class of ergosterol biosynthesis inhibitors and thus differ from the polyene macrolide antibiotics. indeed, it is now believed that the antifungal action of the polyenes, nystatin and amphotericin b, is ...19873319421
[studies of the mechanism of bacterial inactivation after administration of the preparation polchlor k].the purpose of the study was to determine the effect of chloroiodic complex compound with non-ionic surface active substances such as polchlor k on the kinetics of dying, activity of bacterial dehydrogenases and glucosidases as well as ultrastructure of bacterial cells on the example of escherichia coli and staphylococcus aureus. the evaluation of the kinetics of bacteria dying under the preparation activity revealed higher activity against e. coli than s. aureus. it was found that the preparati ...19863325944
on the "culture medium for cultivation of m. leprae" published by dr. laszlo kato. 19873326905
isofluorane inhibits early mouse embryo development in vitro.in vitro development of 2-cell mouse embryos to the blastocyst stage was used to assess the toxicity of isofluorane, nitrous oxide, fentanyl, and meperidine. isofluorane at concentrations similar to those employed during human oocyte recovery for ivf significantly inhibited mouse embryo development. since the other agents were without effect, balanced anesthesia with nitrous oxide and narcotics may be preferable to isofluorane for ivf and gift.19883335266
isofluorane inhibits early mouse embryo development in vitro.in vitro development of 2-cell mouse embryos to the blastocyst stage was used to assess the toxicity of isofluorane, nitrous oxide, fentanyl, and meperidine. isofluorane at concentrations similar to those employed during human oocyte recovery for ivf significantly inhibited mouse embryo development. since the other agents were without effect, balanced anesthesia with nitrous oxide and narcotics may be preferable to isofluorane for ivf and gift.19883335266
the preparation and characterization of cr(iii) and co(iii) complexes of gdp and gtp and their interactions with avian phosphoenolpyruvate carboxykinase.the exchange inert coordination complexes, cr(h2o)4gdp, cr(h2o)4gtp, cr(nh3)4gdp, cr(nh3)4gtp, co(nh3)4gdp, and co(nh3)4gtp have been synthesized and characterized. the lambda and delta coordination isomers of cr(h2o)4gdp, cr(nh3)4gdp, and the four cr(h2o)4gtp isomers have been separated by reverse phase hplc and characterized by their cd spectra. while the isomers of co(nh3)4gtp have not been successfully separated, 31p nmr spectroscopy reveals the presence of the lambda and delta forms. the co ...19883346664
piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall.to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ...19883364584
thermochemosensitivity: augmentation by hyperthermia of cytotoxicity of anticancer drugs against human colorectal cancers, measured by the human tumor clonogenic assay.thermochemosensitivity was examined in vitro by a human tumor clonogenic assay (htca) using specimens obtained surgically from 43 patients with colorectal cancer. we found that the percentages of patients whose cells showed higher sensitivity (greater than 70% inhibition of colony formation) to hyperthermia alone were 29.6 and 55.6% at 42 and 43 degrees c each for 1 h, respectively. similarly, percentages of patients whose cells were sensitive to drugs alone were 13.8, 24.1 and 48.3%, for cis-di ...19883368200
[sensitivity to an amoxicillin-clavulanic acid combination, of deep tonsillar flora isolated in chronic tonsillitis].deep tonsillar flora were identified in tonsils removed from 48 patients, children and adults, with chronic tonsillitis and recurrent sore throats or obstructive hypertrophy. most specimens (38/48) cultured multiple germs (2 to 5 different species) with aerobic and anaerobic gram+ and gram-forms, some being beta-lactamase producers. of the total of 135 strains isolated, 104 were aerobic and 31 anaerobic. the species of aerobic germs most frequently isolated were: apart from alpha-hemolytic strep ...19883369805
human pharmacological investigation of a human recombinant tumor necrosis factor preparation (pac-4d) a phase-i trial.15 patients aged between 24 and 66 years with 10 different malignant tumor diseases were treated with a recombinant human tumor necrosis factor preparation pac-4d in a phase-i trial. the starting dose was 10(5) u pac-4d as an intravenous short infusion. the maximally tolerable dose is around 18 x 10(5) u/m2. as the main clinical side effects were observed: fever, chills, hypertension with subsequent hypotension, lethargy, transient somnolence, headache, neurological deficiency symptoms, nausea a ...19883377652
recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals.examination of 15,844 clinical specimens submitted over 12 years (1973 to 1985) to the anaerobic microbiology laboratories in two military hospitals demonstrated the recovery of anaerobic bacteria in 4,458 (28.1%) specimens. the specimens yielded 6,557 anaerobic isolates (1.47 isolates per specimen). bacteroides spp. accounted for 43% of all isolates; anaerobic gram-positive cocci, 26%; clostridium spp., 7%; and fusobacterium spp., 4%. bacteroides spp. predominated in abscesses, obstetrical and ...19883384929
induction of gene amplification by arsenic.arsenic is a well-established carcinogen in humans, but there is little evidence for its carcinogenicity in animals and it is inactive as an initiator or tumor promoter in two-stage models of carcinogenicity in mice. two arsenic salts (sodium arsenite and sodium arsenate) induced a high frequency of methotrexate-resistant 3t6 cells, which were shown to have amplified copies of the dihydrofolate reductase gene. the ability of arsenic to induce gene amplification may relate to its carcinogenic eff ...19883388020
evidence of estrogen receptors in normal human osteoblast-like cells.in seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. the nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. these are characteristics of biologically active estrogen receptors. pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six ce ...19883388021
the synergistic effect of cefotaxime and desacetylcefotaxime against clinical isolates of anaerobic bacteria.the synergistic interaction of cefotaxime and desacetylcefotaxime against 187 clinically significant anaerobic organisms was investigated. fusobacterium nucleatum, actinomyces odontolyticus, propionibacteria, lactobacilli, peptostreptococci, streptococcus intermedius and veillonella were sensitive to cefotaxime. both eubacterium lentum and streptococcus morbillorum were resistant. the susceptibility of the clostridia varied from 0.125 to greater than 256 mg/l; only 20% of species demonstrated sy ...19883396488
microbial flora of surface versus core tonsillar cultures in recurrent tonsillitis in children.surface and 'core' tonsillar specimens were collected from 60 children, between 2 and 14 years of age, suffering from recurrent tonsillitis, in order to establish the reliability of surface tonsillar swabbing technique and to define the most frequently involved aerobic and anaerobic microorganisms. from the qualitative point of view, the same mixed aerobic and anaerobic flora were obtained in both samples thus demonstrating the reliability of the surface swabbing technique. from the quantitative ...19883397235
comparative studies on the central effects of the angiotensin ii analogue (sar1 azaval3 ile8) at ii.the effects of the newly-synthesized at ii analogue (sar1 azaval3 ile8) at ii were investigated in comparison with the octapeptide at ii and the analogue saralazin (sar1 ala8) at ii, using intracerebroventricular administration, with respect to the following parameters: the level of biogenic monoamines (da, na and 5-ht) and the metabolites hva and 5-hiaa in mouse forebrain; the behaviour of the animals--cataleptogenic actions of mice, ptz convulsive--seizure threshold in mice, apomorphine stereo ...19883407412
atypical invasive external otitis from aspergillus.we report a rare case of invasive external otitis caused by aspergillosis in an elderly nondiabetic patient. amphotericin b therapy was curative. atypical features of the presentation delayed diagnosis. early use of tissue biopsy and culture to guide prompt initiation of therapy is recommended. the clinical spectrum and microbiology of invasive aspergillosis are also reviewed.19883408569
atypical invasive external otitis from aspergillus.we report a rare case of invasive external otitis caused by aspergillosis in an elderly nondiabetic patient. amphotericin b therapy was curative. atypical features of the presentation delayed diagnosis. early use of tissue biopsy and culture to guide prompt initiation of therapy is recommended. the clinical spectrum and microbiology of invasive aspergillosis are also reviewed.19883408569
[immunomodulating properties of khlotazol].a nonsteroidal anti-inflammatory agent khlotazol administered to rats in doses of 40-80 mg/kg for 5 days stimulated synthesis of igm- and igg-antibodies to ram erythrocytes and increased the number of antibody-forming cells (afc) and rosette-forming cells (rfc) in the spleen. when the doses were increased to 160-320 mg/kg, the stimulating effect of the drug on the production of hemagglutinins retained but dose-dependent inhibition of the number of afc and rfc was found. when administered in a do ...19883410037
influence of increasing concentrations of ethanol on food and water intake, body weight, and wheel-running of male sprague-dawley rats.male sprague-dawley rats were used to study the influence of increasing concentrations of ethanol on wheel-running, food and water intake and body weight. animals were housed individually in screen-bottom cages (n = 12) or in activity wheel cages (n = 12). half the animals in each activity condition received an ethanol-free liquid diet, and the remainder received liquid diet in which the concentration of ethanol was increased by 5% of energy intake per week to a maximum of 35%, at the expense of ...19883413194
a hyperthermic syndrome in two subjects with acute hydrocephalus.although intracranial hypertension may cause autonomic disturbances, as well as alterations in the regulation of body temperature, an acute hyperthermic syndrome with autonomic disturbance as a consequence of hydrocephalus has not been described previously. two subjects presented with such a syndrome, with each of several episodes of acute shunt failure and hydrocephalus. with correction of the hydrocephalus, the autonomic disturbances and fever immediately cleared. observations from human and e ...19883415522
[pharmacological correction of experimental alcoholic embryopathy].in the experiments on the progeny of ethanol-exposed rats it was shown that consumption of 15% alcohol instead of water during pregnancy resulted in the worsening of shuttlebox avoidance learning and decrease in succinate dehydrogenase activity in the visual and sensorimotor cortex. these data are indicative of cerebral hypoxia during embryogenesis. the injection of synthetic opioid peptide dalargin during critical periods of development (at the end of embryogenesis and early ontogeny) prevented ...19883416055
influence of coenzyme q10 on doxorubicin uptake and metabolism by mouse myocardial cells in culture. 19883416371
quantification and characterization of enkephalins in the upper part of the cat digestive tract and the coeliac ganglia.the [met]enkephalin, [leu]enkephalin and [met]enkephalin-arg-gly-leu contents of the upper part of the digestive tract (lower oesophageal sphincter, fundus, antrum, pylorus, duodenum, ileum) and coeliac ganglia of the cat were determined and identified. the enkephalin content of all the structures studied, expressed in femtomole/mg of wet tissue, was found to range from 83 to 446 with [met]enkephalin; 19 to 63 with [leu]enkephalin; 2.5 to 13 with [met]enkephalin-arg-gly-leu. in the muscular and ...19883419585
common genetic pools between human populations.a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...19883422217
new attachment formation following controlled tissue regeneration using biodegradable membranes.the present study evaluated the potential for reestablishment of connective tissue attachment after exclusion of the gingival connective tissue and epithelium during healing after periodontal surgery in a dog model. buccal mucoperiosteal flaps were elevated in all four premolar regions in two dogs. the buccal bone was reduced to approximately 25% of its original level on 24 roots. before the elevated flaps were replaced and sutured, membranes were adjusted to cover the exposed root surfaces. on ...19883422287
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