PMID(sorted descending)
anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.human mesenchymal stem cells (hmscs) are multipotent by nature and are originally isolated from bone marrow. in light of a future application of hmscs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study to gain information about the behavior of hmsc in an anaerobic system and the response in interaction with oral bacterial pathogens.201324223777
effect of controlled-release periochip™ on clinical and microbiological parameters in patients of chronic periodontitis.the aim of the present study was to evaluate and compare the clinical and microbiological effectiveness of periochip™ as an adjunct to scaling and root planing (srp) with srp alone in patients with chronic periodontitis.201324174754
antimicrobial photodynamic efficiency of novel cationic porphyrins towards periodontal gram-positive and gram-negative pathogenic bacteria.the gram-negative aggregatibacter actinomycetemcomitans and fusobacterium nucleatum are major causative agents of aggressive periodontal disease. due to increase in the number of antibiotic-resistant bacteria, antimicrobial photodynamic therapy (apdt) seems to be a plausible alternative. in this work, photosensitization was performed on gram-positive and gram-negative bacteria in pure culture using new-age cationic porphyrins, namely mesoimidazolium-substituted porphyrin derivative (imp) and pyr ...201424164211
cetylpyridinium chloride suppresses gene expression associated with halitosis.halitosis is a common complaint affecting the majority of the population. mouthrinses containing cetylpyridinium chloride (cpc) have been used as oral hygiene aids to suppress oral malodor. although the clinical efficacy of these mouthrinses has been well-documented, the mechanism whereby cpc reduces malodor is less-well-understood. we hypothesized that cpc suppresses expression of the genes (mgl and cdl) and enzymes responsible for methyl mercaptan (ch3sh) and hydrogen sulfide (h2s) production ...201324112735
differential effect of autoinducer 2 of fusobacterium nucleatum on oral streptococci.autoinducer 2 (ai-2) is a quorum sensing molecule and plays an important role in dental biofilm formation, mediating interspecies communication and virulence expression of oral bacteria. fusobacterium nucleatum connects early colonizing commensals and late colonizing periodontopathogens. f. nucleatum ai-2 and quorum sensing inhibitors (qsis) can manipulate dental biofilm formation. in this study, we evaluated the effect of f. nucleatum ai-2 and qsis on biofilm formation of streptococcus gordonii ...201324112724
fusobacterium nucleatum induces cytokine production through toll-like-receptor-independent determine whether fusobacterium nucleatum's ability to invade cells allows the bacteria to activate pro-inflammatory response through cytosolic pattern recognition receptors, independent of surface toll-like receptors (tlrs).201424102075
oral community interactions of filifactor alocis in vitro.filifactor alocis is a gram positive anaerobe that is emerging as an important periodontal pathogen. in the oral cavity f. alocis colonizes polymicrobial biofilm communities; however, little is known regarding the nature of the interactions between f. alocis and other oral biofilm bacteria. here we investigate the community interactions of two strains of f. alocis with streptococcus gordonii, fusobacterium nucleatum, porphyromonas gingivalis and aggregatibacter actinomycetemcomitans, organisms w ...201324098460
myocardial infection due to fusobacterium nucleatum.fusobacterium nucleatum is an anaerobic gram-negative bacillus, which inhabits the oropharynx, gastrointestinal tract, and female genital tract. infections classically affect the head and neck. we report a patient with a myocardial mass due to f. nucleatum, initially thought to be a neoplasm, and discuss anaerobic cardiac infections.201324094834
a chlorhexidine-loaded biodegradable cellulosic device for periodontal pockets treatment.absorbent points widely used in endodontic therapy were transformed into bioresorbable chlorhexidine delivery systems for the treatment of the periodontal pocket by preventing its recolonization by the subgingival microflora. these paper points (pps) were first oxidized to promote their resorption, then grafted with β-cyclodextrin (cd) or maltodextrin (md) in order to achieve sustained delivery of chlorhexidine. we investigated the oxidation step parameters through the time of reaction and the n ...201424090988
antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms.the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ...201324077540
salivary detection of periodontopathic bacteria in fanconi's anemia patients.fanconi's anemia (fa) is characterized by bone marrow failure and can lead to infections such as periodontal disease. the aim of this study was to compare the prevalence of four periodontopathic bacteria including aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum and treponema denticola in saliva samples from children with and without fa. paraffin-stimulated saliva samples were collected from 71 children and adolescents, aged 6-18 years old. the samples wer ...201324055631
dna-based adaptive immunity protect host from infection-associated periodontal bone resorption via recognition of porphyromonas gingivalis virulence component.porphyromonas gingivalis (pg) is one of a constellation of oral organisms associated with human chronic periodontitis. while adaptive immunity to periodontal pathogen proteins has been investigated and is an important component of periodontal bone resorption, the effect of periodontal pathogen dna in eliciting systemic and mucosal antibody and modulating immune responses has not been investigated.201424051159
quantitative analysis of periodontal pathogens present in the saliva of geriatric present, information regarding periodontal disease in geriatric patients is scarce. the purpose of this study was to quantify the periodontal pathogens present in the saliva of korean geriatric patients and assess the relationship between the bacterial levels and the periodontal condition.201324040571
the effects of stress hormones on growth of selected periodontitis related bacteria.the focus of this study was to examine in vitro the effects of stress hormones (catecholamines: epinephrine, norepinephrine, dopamine and hydrocortisone: cortisol) on the growth of four anaerobic species of periodontitis-related bacteria (fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and tannerella forsythia) and one facultative anaerobic species (eikenella corrodens). bacterial growth was determined by two different methods: fluorescence in situ hybridization (fish), ...201324036419
synergistic growth effect among bacteria recovered from root canal infections.the objective of this study was to determine the ecological relationships between bacterial species that colonize infected root canals. root canal bacteria recovered from one patient with pulp canal necrosis were evaluated in vitro for synergistic and antagonistic activities determined by mono and co-culture growth kinetics and the production of bacteriocin-like substances using the double layer diffusion method. peptostreptococcus prevotii triggered a significant increase of fusobacterium nucle ...201124031714
progression of periodontal inflammation in adolescents is associated with increased number of porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, and fusobacterium nucleatum.the study aims to evaluate the change of related subgingival periodontopathogens among different stage of gingivitis in adolescent and assess the relationship between periodontopathogens and the progression of periodontal inflammation.201424025042
the predominant bacteria isolated from radicular detect predominant bacteria associated with radicular cysts and discuss in light of the literature.201324011184
porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of prommp9 and its activation.recent epidemiological studies have revealed a significant association between periodontitis and oral squamous cell carcinoma (oscc). furthermore, matrix metalloproteinase 9 (mmp9) is implicated in the invasion and metastasis of tumour cells. we examined the involvement of porphyromonas gingivalis, a periodontal pathogen, in oscc invasion through induced expression of prommp and its activation. prommp9 was continuously secreted from carcinoma sas cells, while p. gingivalis infection increased pr ...201323991831
[evaluation on antimicrobial efficacy of fufang bingpeng irrigant: an in vitro study].to evaluate the antimicrobial efficacy of fufang bingpeng irrigant and bingpeng irrigant on four endodontic pathogen bacteria.201323991570
bacterial community development in experimental gingivitis.current knowledge of the microbial composition of dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description of oral microbial communities. this study used 454-pyrosequencing of the v1-v3 region of 16s rrna genes (approximately 500 bp), and bacterial culture, to characterize the composition of plaque during the transition from periodontal health to gingivitis. a total of 20 healthy volunteers abstained from oral hygiene ...201323967169
fusobacterium nucleatum potentiates intestinal tumorigenesis and modulates the tumor-immune microenvironment.increasing evidence links the gut microbiota with colorectal cancer. metagenomic analyses indicate that symbiotic fusobacterium spp. are associated with human colorectal carcinoma, but whether this is an indirect or causal link remains unclear. we find that fusobacterium spp. are enriched in human colonic adenomas relative to surrounding tissues and in stool samples from colorectal adenoma and carcinoma patients compared to healthy subjects. additionally, in the apc(min/+) mouse model of intesti ...201323954159
fusobacterium nucleatum promotes colorectal carcinogenesis by modulating e-cadherin/β-catenin signaling via its fada adhesin.fusobacterium nucleatum (fn) has been associated with colorectal cancer (crc), but causality and underlying mechanisms remain to be established. we demonstrate that fn adheres to, invades, and induces oncogenic and inflammatory responses to stimulate growth of crc cells through its unique fada adhesin. fada binds to e-cadherin, activates β-catenin signaling, and differentially regulates the inflammatory and oncogenic responses. the fada-binding site on e-cadherin is mapped to an 11-amino-acid re ...201323954158
characterization and in vitro properties of oral lactobacilli in breastfed infants.lactobacillus species can contribute positively to general and oral health and are frequently acquired by breastfeeding in infancy. the present study aimed to identify oral lactobacilli in breast and formula-fed 4 month-old infants and to evaluate potential probiotic properties of the dominant lactobacillus species detected. saliva and oral swab samples were collected from 133 infants who were enrolled in a longitudinal study (n=240) examining the effect of a new infant formula on child growth a ...201323945215
genotype is an important determinant factor of host susceptibility to periodontitis in the collaborative cross and inbred mouse populations.periodontal infection (periodontitis) is a chronic inflammatory disease, which results in the breakdown of the supporting tissues of the teeth. previous epidemiological studies have suggested that resistance to chronic periodontitis is controlled to some extent by genetic factors of the host. the aim of this study was to determine the phenotypic response of inbred and collaborative cross (cc) mouse populations to periodontal bacterial challenge, using an experimental periodontitis model. in this ...201323937452
multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the vitek® ms system.accurate and timely identification of anaerobic bacteria is critical to successful treatment. classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) is an identification method that can provide rapid identification of anaerobes. we present a multi-centre study assessing the clinical performance of the vitek(®) ms in the identification ...201423927597
oral fusobacterium nucleatum subsp. polymorphum binds to human salivary α-amylase.fusobacterium nucleatum acts as an intermediate between early and late colonizers in the oral cavity. in this study, we showed that f. nucleatum subsp. polymorphum can bind to a salivary component with a molecular weight of approximately 110 kda and identified the protein and another major factor of 55 kda, as salivary α-amylase by time-of-flight mass spectrometry and immuno-reactions. salivary α-amylase is present in both monomeric and dimeric forms and we found that formation of the dimer depe ...201323906425
a periodontal pathogen treponema denticola hijacks the fusobacterium nucleatum-driven host response.periodontitis is a polymicrobial disease that arises from the dysbiosis of the plaque biofilm. to study polymicrobial interactions with gingival epithelial cells, the oral commensal fusobacterium nucleatum and the periodontal pathogen treponema denticola were chosen due to their opposing effects on the expression of human beta-defensins (hbds) and interleukin (il)-8 in gingival epithelial cells. immortalized gingival epithelial hok-16b cells were infected with either f. nucleatum or t. denticola ...201323897119
photodynamic biofilm inactivation by sapyr--an exclusive singlet oxygen photosensitizer.prevention and control of biofilm-growing microorganisms are serious problems in public health due to increasing resistances of some pathogens against antimicrobial drugs and the potential of these microorganisms to cause severe infections in patients. therefore, alternative approaches that are capable of killing pathogens are needed to supplement standard treatment modalities. one alternative is the photodynamic inactivation of bacteria (pib). the lethal effect of pib is based on the principle ...201323891675
relationship between halitosis and periodontal disease - associated oral bacteria in tongue coatings.the objective of our study was to investigate the relationship between halitosis and oral bacteria in tongue coating (tc) and saliva samples from patients with halitosis, and to evaluate the effect of tongue cleaning on halitosis.201423890391
endobronchial lesion due to pulmonary fusobacterium nucleatum infection in a child.clinically significant infections due to the members of the genus fusobacterium are rare. the clinical manifestations of pulmonary fusobacterium nucleatum infections range from simple aspiration pneumonia to severe diseases as necrotizing pneumonia, lung abscess, and empyema. endobronchial lesions and obstructions are rarely seen in children and are often a misdiagnosed result in delay of definitive treatment. here, we report a case of endobronchial lesion due to pulmonary f. nucleatum infection ...201423868895
halitosis vaccines targeting foma, a biofilm-bridging protein of fusobacteria nucleatum.halitosis (bad breath) is estimated to influence more than half of the world's population with varying degree of intensity. more than 85% of halitosis originates from oral bacterial infections. foul-smelling breath mainly results from bacterial production of volatile sulfur compounds (vscs) such as hydrogen sulfide and methyl mercaptan. to date, major treatments for elimination of oral malodor include periodontal therapy combined with antibiotics or antimicrobial agents, and mechanical approache ...201323865430
microbiological diversity of peri-implantitis biofilm by sanger examine the microbial diversity associated with implants with or without peri-implantitis and to identify differences between the bacterial communities in these clinical conditions.201423845046
effects of c-reactive protein on the neutrophil respiratory burst in vitro.this study determined the influence of physiologically relevant concentrations of c-reactive protein (crp) on reactive oxygen species (ros) production by neutrophils. neutrophils from healthy individuals were incubated with soluble pentameric crp prior to tlr stimulation with fusobacterium nucleatum, or fcγr stimulation with igg-opsonised staphylococcus aureus or heat-aggregated igg. ros generation by unstimulated cells and those after stimulation were determined using luminol, isoluminol and lu ...201423839528
a new type of na(+)-driven atp synthase membrane rotor with a two-carboxylate ion-coupling motif.the anaerobic bacterium fusobacterium nucleatum uses glutamate decarboxylation to generate a transmembrane gradient of na⁺. here, we demonstrate that this ion-motive force is directly coupled to atp synthesis, via an f₁f₀-atp synthase with a novel na⁺ recognition motif, shared by other human pathogens. molecular modeling and free-energy simulations of the rotary element of the enzyme, the c-ring, indicate na⁺ specificity in physiological settings. consistently, activity measurements showed na⁺ s ...201323824040
characterization and application of a flow system for in vitro multispecies oral biofilm formation.bacteria in the oral cavity grow in the form of biofilms; these structures are subject to constant saliva or gingival crevicular fluid flow conditions. the aims of this study were: (i) to develop and to characterize an in-vitro biofilm model with oral bacteria growing under flow and shear conditions; and (ii) to demonstrate the usefulness of the model for evaluating the activity of three antiplaque agents.201423815431
evaluation of the host response in various models of induced periodontal disease in mice.the aim of this study is to characterize and evaluate the host response caused by three different models of experimental periodontitis in mice.201423805811
microbiological evaluation of bacterial and mycotic seal in implant systems with different implant-abutment interfaces and closing torque values.the aim of this study was to evaluate the possible leakage of 3 species of bacteria (streptococcus sanguinis, fusobacterium nucleatum, and actinomyces odontolyticus) and of candida albicans and candida glabrata in osseointegrated implants with different implant-abutment interface (iai) geometry.201323782848
development of a novel multi-triplex qpcr method for the assessment of bacterial community structure in oral populations.gingivitis and dental caries are two of the most predominant diseases in humans. both conditions are easily treated with the removal of the plaque biofilm by brushing or the use of oral hygiene products. in both cases, pathogenic taxa found within the plaque biofilm are the causal agents of the disease. actinomyces naeslundii, fusobacterium nucleatum and prevotalla intermedia have all been implicated in the development of gingivitis, while streptococcus mutans is the main organism associated wit ...201023766283
the connection between ruptured cerebral aneurysms and odontogenic bacteria.patients with ruptured saccular intracranial aneurysms have excess long-term mortality due to cerebrovascular and cardiovascular diseases compared with general population. chronic inflammation is detected in ruptured intracranial aneurysms, abdominal aortic aneurysms and coronary artery plaques. bacterial infections have been suggested to have a role in the aetiology of atherosclerosis. bacteria have been detected both in abdominal and coronary arteries but their presence in intracranial aneurys ...201323761916
interactions between lactobacillus crispatus and bacterial vaginosis (bv)-associated bacterial species in initial attachment and biofilm formation.certain anaerobic bacterial species tend to predominate the vaginal flora during bacterial vaginosis (bv), with gardnerella vaginalis being the most common. however, the exact role of g. vaginalis in bv has not yet been determined. the main goal of this study was to test the hypothesis that g. vaginalis is an early colonizer, paving the way for intermediate (e.g., fusobacterium nucleatum) and late colonizers (e.g., prevotella bivia). theoretically, in order to function as an early colonizer, spe ...201323739678
effects of short-chain fatty acids on actinomyces naeslundii biofilm formation.actinomyces naeslundii is an early colonizer and has important roles in the development of the oral biofilm. short-chain fatty acids (scfa) are secreted extracellularly as a product of metabolism by gram-negative anaerobes, e.g. porphyromonas gingivalis and fusobacterium nucleatum; and the scfa may affect biofilm development with interaction between a. naeslundii and gram-negative bacteria. our aim was to investigate the effects of scfa on biofilm formation by a. naeslundii and to determine the ...201323731652
necrotizing pneumonia in children: report of 41 cases between 2006 and 2011 in a french tertiary care center.forty-one children hospitalized for necrotizing pneumonia were retrospectively analyzed. necrotizing pneumonia represented 0.8% of community-acquired pneumonia and 6% of hospitalized community-acquired pneumonia. the chest radiograph revealed necrosis on admission in onethird of cases. twenty-one cases (51%) were documented, including 13 staphylococcus aureus, all panton-valentine leukocidin positive, 7 streptococcus pneumoniae and 1 fusobacterium nucleatum.201323722529
porphyromonas gingivalis, treponema denticola and toll-like receptor 2 are associated with hypertensive disorders in placental tissue: a case-control explore the associations between the presence of periodontal pathogens and the expression of toll-like receptors (tlr-2 and tlr-4) in the placental tissue of patients with hypertensive disorders compared to the placentas of healthy normotensive patients.201323711357
in vivo study of the initial bacterial adhesion on different implant materials.biofilm formation on implant materials plays a major role in the aetiology of periimplantitis. the aim of this study was to examine in vivo the initial bacterial adhesion on six different implant materials.201323694907
development of quantitative real-time pcr primers for detecting 42 oral bacterial this study, we introduced species-specific quantitative real-time pcr (qpcr) primers designed based on a dna-dependent rna polymerase beta-subunit gene (rpob) for detecting 42 oral bacterial species. the specificity of the qpcr primers was confirmed by conventional pcr with the genomic dnas of 73-79 strains regarding 73-75 bacterial species including the type strain for the target species. the standard curves revealed the lower detection limits of 42 bacterial species-specific qpcr primers ra ...201323689247
phototoxic effect of blue light on the planktonic and biofilm state of anaerobic periodontal pathogens.the purpose of this study was to compare the phototoxic effects of blue light exposure on periodontal pathogens in both planktonic and biofilm cultures.201323678390
oxygen deprivation affects the antimicrobial action of ll-37 as determined by microplate real-time kinetic measurements under anaerobic conditions.some agents, including escherichia coli and group a streptococcus pyogenes cause infections in oxygen depleted sites. ll-37 is a human host defence peptide shown previously to play an important role in controlling infections caused by these bacteria. however, the effect of oxygen levels on the antimicrobial activity of ll-37 remains obscure. in order to test the effect of oxygen (or lack thereof) on ll-37's activity against e. coli and s. pyogenes, a method for adapting commonly used microtiter ...201323664904
validation of antibiotic efficacy on in vitro subgingival biofilms.systemic antibiotics are often used as adjunctive treatment modalities for periodontal diseases. testing of antimicrobial efficacy can be relevant only if the bacteria are in the form of biofilms rather than the planktonic state, and at concentrations of physiologic relevance, i.e., reachable in the periodontal pocket. the aim of the present study is to test the antimicrobial efficacy of five common antibiotic schemes at physiologically relevant concentrations on a multispecies in vitro biofilm ...201423659420
effect of metalloporphyrins on red autofluorescence from oral bacteria.the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ...201323659237
detection of eight periodontal microorganisms and distribution of porphyromonas gingivalis fima genotypes in chinese patients with aggressive periodontitis.the microbiologic feature of aggressive periodontitis (agp) in chinese patients has not yet been determined. this study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the porphyromonas gingivalis fima genotype in a cohort of chinese patients with agp.201423646850
short-term microbiological effects of scaling and root planing and essential-oils mouthwash in chinese assess the short-term effect of scaling and root planing (srp) and essential-oils mouthwash on the levels of specific bacteria in chinese adults.201323645178
the protective effect of recombinant foma-expressing lactobacillus acidophilus against periodontal infection.a number of studies have shown that the outer membrane protein foma found in fusobacterium nucleatum demonstrates great potential as an immune target for combating periodontitis. lactobacillus acidophilus is a useful antigen delivery vehicle for mucosal immunisation, and previous studies by our group have shown that l. acidophilus acts as a protective factor in periodontal health. in this study, making use of the immunogenicity of foma and the probiotic properties of l. acidophilus, we construct ...201323644821
the lipid bilayer-inserted membrane protein bama of escherichia coli facilitates insertion and folding of outer membrane protein a from its complex with skp.folding of β-barrel membrane proteins, either from a urea-unfolded form or from chaperone-bound aqueous forms, has been characterized for pure lipid bilayers. the impact of preinserted integral proteins from biomembranes has not been examined in biophysical comparisons, but this knowledge is important for the characterization of protein assembly machinery in membranes to distinguish specific effects from unspecific effects. here, folding was studied for a β-barrel membrane protein, outer membran ...201323641708
evaluation of periodontal pathogens in amniotic fluid and the role of periodontal disease in pre-term birth and low birth weight.pre-term birth and/or low birth weight (ptlbw) is a serious problem in developing countries. the absence of known risk factors in ≈ 50% of ptlbw cases has resulted in a continued search for other causes. the aim of this study was to examine the effect of periodontitis on pregnancy outcomes.201223638858
comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: a clinical and microbiologic study.gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations.201323633772
mobile microbiome: oral bacteria in extra-oral infections and inflammation.the link between oral infections and adverse systemic conditions has attracted much attention in the research community. several mechanisms have been proposed, including spread of the oral infection due to transient bacteremia resulting in bacterial colonization in extra-oral sites, systemic injury by free toxins of oral pathogens, and systemic inflammation caused by soluble antigens of oral pathogens. mounting evidence supports a major role of the systemic spread of oral commensals and pathogen ...201323625375
a case of adolescent pelvic inflammatory disease caused by a rare bacterium: fusobacterium nucleatum.pelvic inflammatory disease (pid) is a polymicrobial infection that typically occurs in sexually active females. pid is rare in premenarchal and/or noncoital young women; however, there are case reports of pid occurring in virginal females. we present the first reported case of pid associated with f. nucleatum. in this unusual case of pid in an adolescent, the responsible organism may have originated from the patient's oral cavity.201323619431
microcalorimetric determination of the effects of amoxicillin, metronidazole, and their combination on in vitro biofilm.the mechanism of action of adjuvant antibiotic therapy in the treatment of peri-implantitis is not well understood. the aim of this study is to investigate antibiotic susceptibility of an in vitro biofilm by isothermal microcalorimetry (imc).201423594193
suppression of t-cell chemokines by porphyromonas gingivalis.porphyromonas gingivalis is a major pathogen in periodontal disease and is associated with immune dysbiosis. in this study, we found that p. gingivalis did not induce the expression of the t-cell chemokine ip-10 (cxcl10) from neutrophils, peripheral blood mononuclear cells (pbmcs), or gingival epithelial cells. furthermore, p. gingivalis suppressed gamma interferon (ifn-γ)-stimulated release of ip-10, itac (cxcl11), and mig (cxcl9) from epithelial cells and inhibited ip-10 secretion in a mixed i ...201323589576
advancement in the routine identification of anaerobic bacteria by maldi-tof mass spectrometry.we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) biotyper as a tool for the identification of anaerobic bacteria compared with 500 base-pair (bp) 16s ribosomal ribonucleic acid (rrna) gene sequencing analysis, which is considered to be the "gold standard" method. a total of 484 anaerobic bacteria were retrieved from the clinical specimens of 318 pediatric patients. molecular identification resulted in 18 genera and 51 species. the most prev ...201323584672
antimicrobial activity of ethanol extracts of laminaria japonica against oral microorganisms.laminaria japonica is a brown alga, which is consumed widely in korea, japan, and china. this study investigated the antimicrobial activity of ethanol extracts of l. japonica against oral microbial species to assess the possible application of l. japonica extracts in dental care products. the minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined in culture medium using a microdilution method. the mics of ethanol extracts of l. japonica with oral stre ...201323583539
quantitative real-time pcr combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model.differentiation of live and dead cells is an important challenge when using molecular diagnosis for microbial identification. this is particularly relevant when bacteria have been exposed to antimicrobial agents. the objective of this study was to test a method using quantitative real-time polymerase chain reaction (qpcr) combined with propidium monoazide (pma), developed for the selective quantification of viable p. gingivalis, a. actinomycetemcomitans, f. nucleatum and total bacteria in an in ...201423581569
lemierre's syndrome: a rare case of pulmonic valve vegetation.lemierre's syndrome is an uncommon complication of pharyngitis commonly associated with an anaerobic gram negative bacterium, fusobacterium necrophorum. the syndrome usually affects young healthy adults with the mean age of 20 and is characterized by recent pharyngitis followed by ipsilateral internal jugular vein thrombosis and septic thromboembolism. the treatment is at least 6 weeks of antibiotics; the role of anticoagulation is unclear. the following presentation is a case of lemierre's synd ...201323573433
host susceptibility to periodontitis: mapping murine genomic susceptibility to periodontal infection is controlled by genetic factors. as a step toward identifying and cloning these factors, we generated an a/j x balb/cj f2 mouse resource population. a genome-wide search for quantitative trait loci (qtl) associated with periodontitis was performed. we aimed to quantify the phenotypic response of the progenies to periodontitis by microct analysis, to perform a genome-wide search for qtl associated with periodontitis, and, finally, to suggest candidate ...201323539559
an investigation of the presence of specific anaerobic species in necrotic primary teeth.different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. the aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (pcs) and root canals (rcs) of infected primary teeth using pcr methods. paired pc and rc samples were collected from 15 subjects and analyzed by pcr for the presence of filifactor alocis, fusobacterium nucleatum, parvimonas micra, porphyromonas endod ...201323538426
in vitro activity of biapenem plus rpx7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria.biapenem is a carbapenem being developed in combination with rpx7009, a new inhibitor of serine β-lactamases. biapenem was tested alone and in combination with fixed concentrations of rpx7009 by agar dilution against 377 recent isolates of anaerobes. a separate panel of 27 isolates of bacteroides spp. with decreased susceptibility or resistance to imipenem was also tested. comparator drugs included meropenem, piperacillin-tazobactam, ampicillin-sulbactam, cefoxitin, ceftazidime, metronidazole, c ...201323529731
antibacterial effect of different root canal sealers on three bacterial species.bacteria persisting in the root canal system may thwart endodontic therapy. it is therefore interesting to know whether clinically available root canal sealers have an antimicrobial effect. the objective of the present in vitro study was to investigate the antibacterial effect of various sealers on the endodontologically detectable species enterococcus faecalis (e. faecalis), fusobacterium nucleatum (f. nucleatum) and porphyromonas gingivalis (p. gingivalis).201323523285
antibacterial activity of essential oils against periodontal pathogens: a qualitative systematic review.periodontal diseases are among the most common infectious diseases that lead to the destruction of periodontal tissues. anaerobic gram-negative bacteria (aggregatibacter actinomecetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum...) isolated from periodontal lesions, have been shown to be related to the onset and progression of periodontal disease. given the incidence of periodontitis, increased resistance of oral bacteria to antibiotics and adverse effects of some antibacterial ag ...201223513511
peptides: β-cyclodextrin inclusion compounds as highly effective antimicrobial and anti-epithelial proliferation agents.the use of antimicrobial peptides (amps) as therapeutic agents for periodontal infections has great advantages, such as broad spectrum of action, low toxicity, and limited bacterial resistance. however, their practical use is limited because of the large amount of peptide required to exercise the microbicidal function.201323510146
comparative microbial analysis of paired amniotic fluid and cord blood from pregnancies complicated by preterm birth and early-onset neonatal sepsis.16s rrna-based genomic analyses have revolutionized our understanding of infectious diseases. many cases which were recognized as "idiopathic" are now known to have an infectious etiology. here, we present a proof-of-concept study to examine the microbial link between intra-amniotic infection (iai) and early-onset neonatal sepsis (eons).201323437088
identification of a 43-kda outer membrane protein of fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other fusobacterium species.a pair of primers was designed in an attempt to amplify outer membrane protein (omp) gene of fusobacterium necrophorum based on nucleotide sequence of the omp of fusobacterium nucleatum. further analysis was performed to characterize its molecular properties and phylogeny in the genus fusobacterium. we identified a predicated 43kda outer membrane protein (43k omp) in f. necrophorum, which showed the same properties as other pore-forming proteins of gram-negative anaerobic bacteria according to a ...201323433684
microbiology of the gut of the kola nut weevil, balanogastris kolae.reports have shown that many insects have microbes in their gut system. gut microbes are very important for insect vitality and much of their nutrition is derived from products of microbial metabolism. the habitat of balanogastris kolae (desbrocher des loges) (coleoptera: curculionidae) suggests that they possess the ability to digest varieties of sugars particularly starch and protein materials present in the kola nut, cola nitida schott & endlicher (malvales: malvaceae). the aim of this study ...201223421598
regulation of visfatin by microbial and biomechanical signals in pdl cells.this in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (pdl) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals.201423404558
macrolides inhibit fusobacterium nucleatum-induced muc5ac production in human airway epithelial cells.fusobacterium nucleatum is one of the most common anaerobic bacteria in periodontitis and is responsible for several extraoral infections, including respiratory tract diseases. in this study, we examined whether f. nucleatum induces mucin secretion in airway epithelial cells. we also examined the effects of macrolides on f. nucleatum-induced mucus production compared with the effects of other antibiotics that exert anti-anaerobic activities. the production of muc5ac, the major core protein of mu ...201323380724
characterization of extracellular polymeric matrix, and treatment of fusobacterium nucleatum and porphyromonas gingivalis biofilms with dnase i and proteinase k.biofilms are organized communities of microorganisms embedded in a self-produced extracellular polymeric matrix (epm), often with great phylogenetic variety. bacteria in the subgingival biofilm are key factors that cause periodontal diseases; among these are the gram-negative bacteria fusobacterium nucleatum and porphyromonas gingivalis. the objectives of this study were to characterize the major components of the epm and to test the effect of deoxyribonuclease i (dnase i) and proteinase k.201323372876
folding and stability of outer membrane protein a (ompa) from escherichia coli in an amphipathic polymer, amphipol a8-35.amphipols are a class of amphipathic polymers designed to maintain membrane proteins in aqueous solutions in the absence of detergents. denatured β-barrel membrane proteins, like outer membrane proteins ompa from escherichia coli and foma from fusobacterium nucleatum, can be folded by dilution of the denaturant urea in the presence of amphipol a8-35. here, the folding kinetics and stability of ompa in a8-35 have been investigated. folding is well described by two parallel first-order processes, ...201323370791
in vitro antimicrobial effects of two antihalitosis mouth rinses on oral pathogens and human tongue microbiota.the aim of the study was to compare the antimicrobial activity of a mouth rinse containing chlorhexidine and cetylpyridinium chloride (mr1) with a stannous fluoride-based mouth rinse (mr2) in vitro.201323368885
the surface layer of tannerella forsythia contributes to serum resistance and oral bacterial coaggregation.tannerella forsythia is an anaerobic, gram-negative bacterium involved in the so-called "red complex," which is associated with severe and chronic periodontitis. the surface layer (s-layer) of t. forsythia is composed of cell surface glycoproteins, such as tfsa and tfsb, and is known to play a role in adhesion/invasion and suppression of proinflammatory cytokine expression. here we investigated the association of this s-layer with serum resistance and coaggregation with other oral bacteria. the ...201323357386
characteristics of porphyromonas gingivalis lipopolysaccharide in co-culture with fusobacterium nucleatum.porphyromonas gingivalis is associated with chronic periodontitis and forms multi-species biofilms. they can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. the lipopolysaccharide (lps) of p. gingivalis shows different virulence by growth condition. the purpose of this study was to investigate the characteristics of p. gingivalis lps when co-cultured with fusobacterium nucleatum. ...201323347381
antibody and t cell responses to fusobacterium nucleatum and treponema denticola in health and chronic periodontitis.the characteristics of the t cell response to the members of oral flora are poorly understood. we characterized the antibody and t cell responses to fada and td92, adhesins from fusobacterium nucleatum, an oral commensal, and treponema denticola, a periodontal pathogen, respectively. peripheral blood and saliva were obtained from healthy individuals and patients with untreated chronic periodontitis (cp, n = 11 paris) and after successful treatment of the disease (n = 9). the levels of antigen-sp ...201323335969
clinical and microbiological results following nonsurgical periodontal therapy with or without local administration of piperacillin/tazobactam.we assessed if adjunct administration of piperacillin/tazobactam added clinical and microbiological treatment benefits.201323334268
exploiting preq(1) riboswitches to regulate ribosomal frameshifting.knowing the molecular details of the interaction between riboswitch aptamers and their corresponding metabolites is important to understand gene expression. here we report on a novel in vitro assay to study preq(1) riboswitch aptamers upon binding of 7-aminomethyl-7-deazaguanine (preq(1)). the assay is based on the ability of the preq(1) aptamer to fold, upon ligand binding, into a pseudoknotted structure that is capable of stimulating -1 ribosomal frameshifting (-1 fs). aptamers from three diff ...201323327288
dna binding to hydroxyapatite: a potential mechanism for preservation of microbial dna.molecular methods are increasingly being deployed for analysis of the microbial flora in the root canal. such methods are based on the assumption that recovered dna is associated with the active endodontic infection, yet paleomicrobiology research is based on the recovery of ancient dna from centuries-old tooth and bone samples, which points to considerable longevity of the dna molecule in these tissues. the main component of dentin and bone is the mineral hydroxyapatite. this study assessed dna ...201323321233
bacterial adhesion on the titanium and zirconia abutment surfaces.microorganisms harboring the oral cavity, mainly those related to periodontal diseases, are the most potential etiologic factor of failure in long-term implant treatment. the material used for abutment components may influence the adhesion and colonization of microbial species. the aim of this in vivo investigation was to evaluate the biofilm formation on machined (mpt) or cast titanium (cpt) and zirconia abutments (zc).201423316996
oral epithelial cell responses to multispecies microbial biofilms.this report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. streptococcus gordonii (sg)/s. oralis (so)/s. sanguinis (ss) and sg/fusobacterium nucleatum (fn)/porphyromonas gingivalis (pg) biofilms elicited significantly elevated levels of il-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. only ...201323300185
[expression of cytokines in dentogingival sulcus in patients after dental implantation and during development of peri-implantitis].determination of cytokine content in various areas of dentition in patients with peri-implantitis associated with parodontopathogenic bacteria species of i and ii order.201223297645
method to quantify live and dead cells in multi-species oral biofilm by real-time pcr with propidium monoazide.real-time pcr (qpcr) is a widely used technique in analysing environmental and clinical microbiological samples. however, its main limitation was its inability to discriminate between live and dead cells.recently, propidium monoazide (pma) together with qpcr has been used to overcome this problem, with good results for different bacterial species in different types of samples.our objective was to implement this technique for analysing mortality in multi-species oral biofilms formed in vitro with ...201323289803
variations in the oral anaerobic microbial flora in relation to pregnancy.pregnancy gingivitis is a major oral infection. periodontium acts as a reservoir of inflammatory mediators and sub gingival biofilms of bacteria.201223285437
validation of a quantitative real-time pcr assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of an in vitro subgingival biofilm model.subgingival biofilms are the prime etiological factor of periodontal disease. owing to their complex polymicrobial nature, quantification of individual bacterial species within the biofilm for research and diagnostic purposes can be methodologically challenging. the aims of this study were to establish a quantitative real-time pcr (qpcr) assay to quantify the bacteria used in our 10-species in vitro 'subgingival' biofilm model and to compare the quantitative outcome with fluorescence microscopy ...201223278531
fusobacterium nucleatum subsp. fusiforme gharbia and shah 1992 is a later synonym of fusobacterium nucleatum subsp. vincentii dzink et al. 1990.on the basis of the dna-dna hybridization patterns and phenotypic characteristics, fusobacterium nucleatum was classified into five subspecies. previous studies have suggested that f. nucleatum subsp. vincentii is genetically similar to f. nucleatum subsp. fusiforme. the aim of this study was to investigate the possibility of classifying these two subspecies into a single subspecies by phylogenetic analysis using a single sequence (24,715 bp) concatenated 22 housekeeping genes of eight f. nuclea ...201323263257
virulence mechanism of bacteria in mixed infection: attenuation of cytokine levels and evasion of polymorphonuclear leukocyte phagocytosis.the objective of the present study is to evaluate the effect of bacterial viability on the virulence of mixed infection.201323259412
modulation of quaternary structure and enhancement of ligand binding by the k-turn of tandem glycine riboswitches.most known glycine riboswitches have two homologous aptamer domains arranged in tandem and separated by a short linker. the two aptamers associate through reciprocal "quaternary" interactions that have been proposed to result in cooperative glycine binding. recently, the interaptamer linker was found to form helix p0 with a previously unrecognized segment 5' to the first aptamer domain. p0 was shown to increase glycine affinity, abolish cooperativity, and conform to the k-turn motif consensus. w ...201323249744
antibacterial activity of probiotic candidates for oral health.the aim of this study was to determine the probiotic potential of autochthonous oral lactobacilli. for this, 66 strains were screened for antibacterial activity against two cariogenic strains (streptococcus mutans and actinomyces viscosus) and two periodontopathogenic strains (fusobacterium nucleatum and porphyromonas gingivalis). the inhibitory activity was investigated with the agar overlay technique. positive results led us to explore some mechanisms of action. the ability to produce h(2)o(2) ...201323211763
optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens.the aim of this study was to optimize quantitative (real-time) polymerase chain reaction (qpcr) assays for 8 major periodontal pathogens, i.e. aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, parvimonas micros, porphyromonas gingivalis, prevotella intermedia, tanerella forsythia and treponema denticola, and of the caries pathogen streptococcus mutans.201223199017
purification, crystallization and preliminary x-ray analysis of two hydrogen sulfide-producing enzymes from fusobacterium nucleatum.hydrogen sulfide produced by oral bacteria is responsible for oral malodour. two homologous hydrogen sulfide-producing enzymes, fn1220 and cdl, from fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. x-ray diffraction data were collected from the crystals using a synchrotron-radiation source. the fn1220 crystal belonged to tetragonal space group p4(1)2(1)2 or p4(3)2(1)2 (unit-cell parameters a=b=116.8, c=99.2 å) and the cdl crystal be ...201223192034
prevalence of β-lactamase-producing bacteria in human periodontitis.beta-lactam antibiotics prescribed in periodontal therapy are vulnerable to degradation by bacterial β-lactamases. this study evaluated the occurrence of β-lactamase-positive subgingival bacteria in chronic periodontitis subjects of usa origin, and assessed their in vitro resistance to metronidazole at a breakpoint concentration of 4 μg/ml.201323173872
high-fat diet induces periodontitis in mice through lipopolysaccharides (lps) receptor signaling: protective action of estrogens.a fat-enriched diet favors the development of gram negative bacteria in the intestine which is linked to the occurrence of type 2 diabetes (t2d). interestingly, some pathogenic gram negative bacteria are commonly associated with the development of periodontitis which, like t2d, is characterized by a chronic low-grade inflammation. moreover, estrogens have been shown to regulate glucose homeostasis via an lps receptor dependent immune-modulation. in this study, we evaluated whether diet-induced m ...201223133617
diagnosis and treatment of fusobacterium nucleatum discitis and vertebral osteomyelitis: case report and review of the report.201323132536
toll-like receptor 2-dependent activity of native major outer membrane protein proteosomes of chlamydia trachomatis.chlamydia trachomatis is the most common sexually transmitted bacterial pathogen and the etiologic agent of blinding trachoma. intracellular signaling pathways leading to host cell inflammation and innate immunity to chlamydia include those mediated by toll-like receptors (tlrs) and nucleotide binding oligomerization domain 1 (nod1) protein. in epithelial cells, tlr-dependent signaling contributes to local immune responses via induction of inflammatory mediators. there is evidence that tlr3, tlr ...201323132491
acute chorioamnionitis at term caused by the oral pathogen fusobacterium nucleatum.fusobacterium nucleatum is an oral pathogen associated with preterm birth. presented is a case of acute chorioamnionitis that progressed to maternal sepsis in a term patient with intact membranes. in addition to its role in periodontal disease and preterm birth, our case demonstrates that intrauterine infection with fusobacterium nucleatum can result in severe disease at term.201223115747
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