Publications

TitleAbstractYear
Filter
PMID
Filter
ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.deoxyribonucleic acid from 41 different bacterial species (including gram-negative and gram-positive species) were cleaved by different restriction endonucleases, electrophoresed in agarose and transferred to nylon filters. the dna fragments carrying rrna genes (rdna) were localized by hybridization with a 32p-labelled escherichia coli 16 + 23s rrna probe. a pattern of hybridized fragments was obtained for each dna tested. within a bacterial species (defined as a dna hybridization group), one or ...19862446639
identification of a galactose-binding lectin on fusobacterium nucleatum fn-2.a previous study has suggested that fusobacterium nucleatum fn-2 contains a galactose-binding protein (lectin) on the cell surface (p. a. murray, v. matarese, c. i. hoover, and j. r. winkler, fems microbiol. lett. 40:123-127, 1987). in the present study, the molecular specificity and size of this lectin were investigated by several techniques. whole-cell affinity chromatography with asialofetuin covalently coupled to sepharose 6mb demonstrated that 81% of 3h-labeled f. nucleatum were specificall ...19882451642
characterization of coaggregation between bacteroides gingivalis t22 and fusobacterium nucleatum t18.bacterial adherence is a key factor in the colonization of the oral ecosystem, yet little is known about the mechanisms by which the pathogen bacteroides gingivalis adheres in the periodontal environment. we examined the ability of strains of b. gingivalis to coaggregate with selected microorganisms isolated from the subgingival microbiota of the cynomolgus monkey. a strong interaction was demonstrated between strains of b. gingivalis and fusobacterium nucleatum, whereas less pronounced or no in ...19892478473
ovine and bovine abortion associated with fusobacterium nucleatum. 19892488355
specificity of monoclonal antibodies reactive with fusobacterium nucleatum: effect of formalin fixation.monoclonal antibody specific for fusobacterium nucleatum was reacted with untreated and formalin fixed f. nucleatum cells by an enzyme-linked immunosorbent assay (elisa) and by indirect immunofluorescence. treatment of bacterial cells with formalin destroyed the antigenic determinant responsible for reactivity with this monoclonal antibody in both assays. formalin fixation had no effect on hemagglutination activity (ha) of f. nucleatum cells or reactivity with polyvalent rabbit antiserum in doub ...19852415637
[central pontine myelinolysis, associated with hyperglycemia and hyponatremia: a clinical case].a 68 year old diabetic male was admitted with hyponatremia and developed a "deafferentiation" syndrome. central pontine myelinolysis was suspected after clinical, eeg and brain cat scan. the diagnosis was confirmed at necropsy. the pathogenesis, clinical manifestations and treatment of this disorder are discussed.19892519379
bactericidal concentrations of chlorhexidine-digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times.in vitro inhibitory and bactericidal concentrations in serum of chlorhexidine-digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against bacteroides gingivalis, bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and capnocytophaga sputigena. the minimal inhibitory concentration was assessed by the agar dilution technique. the killing curves and minimal bactericidal concentration of the antim ...19892524581
[chemiluminescence response and phagocytic activity of murine polymorphonuclear leukocytes to various species of oral bacteria].polymorphonuclear leukocytes (pmn) are predominant cells in the gingival crevice and saliva, and may play an important role in oral bacteria. murine peritoneal pmn was used and stimulated with 9 genera, 17 species of oral bacteria, including cariogenic and periodontal pathogens. the pmn response to the bacteria was measured by the luminol mediated chemiluminescence (cl) response and phagocytic activities, and the activities of lysozyme in the reaction medium after the cl response were also measu ...19892489336
lectinlike interactions of fusobacterium nucleatum with human neutrophils.fusobacterium nucleatum expresses lectinlike adherence factors which mediate binding to a variety of human tissue cells. adherence is selectively inhibited by galactose, lactose, and n-acetyl-d-galactosamine. in this study, adherence of f. nucleatum to human peripheral blood polymorphonuclear neutrophils (pmns) was investigated. the results indicated that the fusobacteria adhered to live and metabolically inactivated or fixed pmns. adherence of f. nucleatum resulted in activation of pmns as dete ...19892553609
unlikely association between clinically apparent herpesvirus infection and coronary incidence at older ages. the framingham heart study.experimental studies in chickens have shown a relationship of a herpesvirus to atherosclerosis. the hypothesis of an association in humans was tested by using data on the history of cold sores and other manifestations of herpes infections reported by 658 male and 919 female participants (ages 58 to 89) in the framingham heart study from 1977 to 1979 and on the prevalence and subsequent 6-year incidence of coronary heart disease (chd). approximately 40% of the men and 52% of the women reported a ...19892556099
absorption of human growth hormone from the rat lung.recombinant methionyl human growth hormone (hgh) was administered intratracheally to adult rats, and serum concentrations of immunoreactive hgh were measured for up to 24 h. the mean absolute bioavailability was approximately 36% after 18h and was similar for doses of 0.75, 1.5, and 3 mg/kg. peak serum hgh concentrations occurred at approximately 6 h after dosing. tritiated hgh (3h-hgh) was used to follow the clearance of hormone from the lungs. disappearance was linear with time and by 24 h app ...19892562650
treatment of mouse thymus cells with phosphatidylinositol-specific phospholipase c preferentially abrogates their reactivity with autoantibodies to the thy-1 antigen.thy-1 molecules have been shown to become anchored to the membrane of thymocytes and of t-cells via a phosphatidylinositol link. in the present study the intensity of immunofluorescent staining of mouse thymus cells by monoclonal xenoantibodies and alloantibodies specific for the thy-1.2 determinant was significantly reduced following exposure of the cells to phosphatidylinositol-specific phospholipase c (pi-plc). the majority of the pi-plc-treated thymus cells retained, however, some reactivity ...19892563957
utilization of amino acids and peptides by fusobacterium nucleatum.fusobacterium nucleatum strains atcc 10953, fevl, f1, f3, and f6 utilized amino acids, in particular glutamate, histidine, and aspartate were common to all strains. strain differences were observed in the utilization of threonine, serine, lysine, tyrosine, and methionine, and only strain atcc 10953 utilized all these amino acids. the glutamate and histidine pools were in all cases fully depleted before the other amino acids were attacked and at the same time all strains except 10953 started to u ...19892565597
recognition of fusobacterium nucleatum subgroups fn-1, fn-2 and fn-3 by ribosomal rna gene restriction patterns.dna from representative strains of fusobacterium nucleatum subgroups fn-1, fn-2 and fn-3 was digested with restriction enzymes ecori and taqi and the electrophoretically separated fragments hybridized with a 32p-16s rrna gene probe from e. coli. the rrna gene restriction patterns from dna digested with either enzyme allowed the clustering of strains into the three subgroups. however, taqi digested dna yielded a wider distribution of taxonomically useful bands (ca 0.65 +/- 14.3 kbp) and the patte ...19892575556
the process of baculovirus deproteinization.the localization and function of alkaline protease inside the supervirion capsids (granules, inclusion bodies) of granulosis virus and nuclear polyhedrosis virus have been determined with the help of electron microscopy. it has been shown that deproteinization of baculoviruses proceeds in several steps. the first two stages (the release of virus from supervirion capsids and the release of nucleocapsids from the internal and external coats, were accomplished in the intestine of the insects, while ...19892576597
self reactive delayed type hypersensitivity (dth) induced in mice by syngeneic lymphoblasts.normal and x-irradiated a mice injected with syngeneic concanavalin a (con a)-induced lymphoblasts revealed after challenge with syngeneic lipopolysaccharide (lps)-induced lymphoblasts delayed type hypersensitivity (dth) measured both by footpad swelling and by 125iudr accumulation. mice injected with allogeneic con a-induced lymphoblasts and challenged with syngeneic lps-induced lymphoblasts or vice versa, also generated an appreciable dth response. in contrast, con a-induced blast cells of hum ...19852580776
conformations of intermediates in the folding of the pancreatic trypsin inhibitor.intermediates in the folding pathway of the bovine pancreatic trypsin inhibitor (pti) have been examined by 1h nuclear magnetic resonance (n.m.r.). the intermediates were trapped during the reoxidation and consequent refolding of reduced pti by alkylating free thiols; each intermediate contained different disulphide linkages. the n.m.r. spectra reveal that conformational features of the native protein are present in the intermediate containing just one of the three normal disulphide linkages (30 ...19872443711
[gene cloning of an actinobacillus actinomycetemcomitans y4 antigen that reacts to peripheral blood sera in advanced destructive periodontitis patients].actinobacillus actinomycetemcomitans (a.a.) has been implicated in the etiology of juvenile periodontitis and also of advanced destructive periodontitis (adp). it has been reported that the levels of the igg antibody against the a.a. in the peripheral blood sera of the adp patients were often high as well as those against the bacteroides gingivalis. to clone the genes of antigen reactive to the sera of the adp patients, we constructed a phage library of the a.a. strain y4 dna in lambda l47, whic ...19892533606
characterization of coagulase-negative staphylococci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses.coagulase-negative staphylococci are important nosocomial pathogens. at present, no wholly satisfactory typing scheme exists for these organisms. therefore, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting were assessed as characterization methods. a total of 100 type strains and nontyped isolates representing nine species of coagulase-negative staphylococci were analyzed. each species had a reproducible, characteristic whole-cell banding pattern when analy ...19892584373
differential expression of class ii (dr & dq) antigens by human gingival langerhans' cells and keratinocytes in vitro.the expression of the class ii products dr and dq on human gingival epithelium was examined using immunofluorescence and immunoperoxidase staining. differential expression of class ii antigens was seen in chronic gingivitis in adults, with t6(+) dr(+) cells being more numerous than t6(+) dq(+) cells. the periodontopathic organism fusobacterium nucleatum (fn) induced dq expression on langerhans' cells (lc) during in vitro explant culture of gingival tissue. this effect was mimicked by endotoxin ( ...19872435875
cytologic and bacteriologic evaluation of tracheobronchial aspirates from clinically normal foals.thirty-eight tracheobronchial aspirates (tba) were collected from twenty 1 to 6-month-old foals, which were free of clinical signs of respiratory tract or other infectious disease. we collected tba from 9 of the foals 3 times when they were approximately 8, 16, and 24 weeks old. aspirates were examined cytologically after staining with modified wright-giemsa, gram, toluidine blue, and prussian blue stains. aerobic bacterial culturing was performed on all aspirates. of the 20 initial tba, 4 (20%) ...19892610430
cytologic and bacteriologic evaluation of tracheobronchial aspirates from clinically normal foals.thirty-eight tracheobronchial aspirates (tba) were collected from twenty 1 to 6-month-old foals, which were free of clinical signs of respiratory tract or other infectious disease. we collected tba from 9 of the foals 3 times when they were approximately 8, 16, and 24 weeks old. aspirates were examined cytologically after staining with modified wright-giemsa, gram, toluidine blue, and prussian blue stains. aerobic bacterial culturing was performed on all aspirates. of the 20 initial tba, 4 (20%) ...19892610430
nile red fluorescence response from the aortic arch to serum cholesterol changes.histologic sections of the aortic arches from guinea pigs were stained with nile red to verify the possibility of quantitating the earliest changes in the arterial wall. nile red fluorescence intensity was quantitated by conversion to electric signal. fluorescence in a group fed cholesterol and casein was significantly higher than that in a control group. its dependence on the logarithm of serum cholesterol concentrations between 1.33 and 7.67 mmol/l was found. small fluorescence intensity diffe ...19892612173
nile red fluorescence response from the aortic arch to serum cholesterol changes.histologic sections of the aortic arches from guinea pigs were stained with nile red to verify the possibility of quantitating the earliest changes in the arterial wall. nile red fluorescence intensity was quantitated by conversion to electric signal. fluorescence in a group fed cholesterol and casein was significantly higher than that in a control group. its dependence on the logarithm of serum cholesterol concentrations between 1.33 and 7.67 mmol/l was found. small fluorescence intensity diffe ...19892612173
leukocyte tubuloreticular inclusions in reye's syndrome.tubuloreticular inclusions (tri) have been observed in the rough endoplasmic reticulum of blood lymphocytes and monocytes in two cases of reye's syndrome initiated by influenza infections. tubuloreticular inclusions are seen in these mononuclear leukocytes during the acute phase of illness, but not during convalescence. since tri have been demonstrated in peripheral mononuclear leukocytes in patients with acquired immunodeficiency syndrome, systemic lupus erythematosus, and certain viral infecti ...19852581524
the effects of acetyl-l-carnitine on experimental models of learning and memory deficits in the old rat.experimental models of learning and memory deficits in aged rats can be studied by means of behavioural tests that provide an important tool for evaluating the effect of drugs on these parameters. active and passive avoidance tests showed a clear impairment of learning and memory capacity of old rats. these tests were also used to study the behavioural effect of acetyl-l-carnitine in aged rats. the subchronic treatment with this drug was followed by a significant improvement of acquisition and r ...19892620857
the effects of acetyl-l-carnitine on experimental models of learning and memory deficits in the old rat.experimental models of learning and memory deficits in aged rats can be studied by means of behavioural tests that provide an important tool for evaluating the effect of drugs on these parameters. active and passive avoidance tests showed a clear impairment of learning and memory capacity of old rats. these tests were also used to study the behavioural effect of acetyl-l-carnitine in aged rats. the subchronic treatment with this drug was followed by a significant improvement of acquisition and r ...19892620857
standardisation of quantitative direct gas liquid chromatography for early detection of bacteria in blood cultures.blood cultures with strains of aerobic, facultative and obligate anaerobic bacteria were studied by quantitative direct gas liquid chromatography for early diagnosis of bacteraemias. small amounts of volatile and nonvolatile fatty acids were detected in uninoculated blood cultures. bacteroides fragilis produced acetic (27.6 mumol/ml), propionic (1.0 mumol/ml), isovaleric, (0.6 mumol/ml), lactic (4.5 mumol/ml) and succinic (2.7 mumol/ml) acids after 48 h. blood cultures inoculated with clostridiu ...19892620945
interleukin-1 and interleukin-1 inhibitor production by human adherent cells stimulated with periodontopathic bacteria.this study examined the effect of the putative periodontopathic bacteria bacteroides gingivalis and fusobacterium nucleatum on the production of interleukin-1 (il-1) and il-1 inhibitors by human plastic-adherent mononuclear cells from normal donors. fusobacterium mortiferum was used as a non-oral, non-pathogenic control organism. unstimulated adherent cells spontaneously secreted an il-1 inhibitor, whereas stimulation with b. gingivalis induced the synthesis and secretion of il-1. with both fuso ...19892624558
lipid keratopathy in rabbits. an animal model system.rabbits rendered hypercholesterolemic on a high cholesterol diet while subjected to corneal suture placement to induce neovascularization made useful models for qualitatively and quantitatively studying lipid keratopathy. forty rabbit eyes were subjected to placement of four sutures located between 1 and 4 mm from the limbus. the neovasculature grew at a constant rate of 0.24 mm/day, unaffected by serum cholesterol levels. rabbits fed cholesterol prior to surgery were the earliest to display lip ...19852581537
narcolepsy in a long-haired dachshund.narcolepsy was diagnosed in a three-month old, longhaired dachshund presented with a history of sudden onset of episodes of complete collapse. littermates or immediate ancestors were not affected. numerous daily cataplectic attacks as well as excessive sleepiness were the main clinical features. the dog did not respond to treatment with methylphenidate and imipramine. the clinical signs of the disease disappeared after treatment with dexamphetamine. this report describes a case presentation, fin ...19892634775
[inducing effect of periodontopathic bacteria on activation of macrophage functions and production of interleukin-1 by mouse peritoneal macrophages].periodontal disease is thought to be initiated by a bacterial infection and subsequently developed by immunopathological mechanisms thorough host-parasite interactions. the macrophage and lymphocyte are the major functional cell types in the lesion of the disease and participate in tissue destruction and alteration of the periodontal connective tissue as well as in host defense mechanisms. however, the detailed implications of macrophages in development of the disease is still unclear. the aim o ...19892601596
high-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis.selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. after addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase c18 bond-elut columns and from urine using pre-packed silica extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before inj ...19892613803
[effects of periodontopathic bacterial components on phagocytic activity of rat peritoneal macrophages. examination using elisa].this study examined the phagocytic activity of rat peritoneal resident macrophages to determine the movement of macrophages in local inflammation in periodontal disease. we studied phagocytic activity by enzymelinked immunosorbent assay (elisa) and used the peroxidase-anti-peroxidase soluble complex (pap; soluble immune complex) as a marker in. we also determined the basic conditions of this examination and studied the effects of bacterial components and the supernatants of sonicated periodontop ...19892637908
outer membrane proteins as major antigens of fusobacterium nucleatum.the immunochemical reactions of rabbit polyclonal antibodies directed to different preparations of fusobacterium nucleatum i.e, whole cells, peptidoglycan associated proteins, a peptidoglycan-protein complex and a purified 40 kilodalton (kda) protein, were investigated on outer membrane preparations of fusobacterium species and a restricted number of leptotrichia buccalis after their separation on sodium dodecyl sulphate polyacrylamide gels and electrotransfer to nitrocellulose. all f. nucleatum ...19892627499
cytotoxicity against various cell lines of lipopolysaccharides purified from bacteroides, fusobacterium, and veillonella isolated from infected root canals.the cytotoxicity against two mesenchymal cell lines, l-929 and wi-38, and two epithelial cell lines, kb and hela s-3, of lipopolysaccharide (lps) purified from strains of bacteroides gingivalis, fusobacterium nucleatum, and veillonella parvula isolated from infected root canals was investigated. the inhibition of cell growth by these lps's was considerable for mesenchymal cell lines, but mild for epithelial cell lines. the cytotoxic effect of f. nucleatum lps was the greatest and that of b. ging ...19892639950
[vestibular evoked potentials in "gallus domesticus"].electrophysiological activity in response to linear acceleration stimuli was recorded from gallus domesticus by means of subcutaneous electrodes. this investigation had two purposes: 1) to obtain normative data for our laboratory, and 2) to rule out auditory and somatosensory contributions to the vestibular evoked potentials (vsep). the stimulus consisted of a sigmoid-shaped voltage function generated by a digital-to-analog converter. this signal was amplified, attenuated, and directed to a shak ...19892635851
purification and partial characterization of a major outer-membrane protein of fusobacterium nucleatum.the major outer-membrane proteins of 40-41 kda were identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) in fusobacterium nucleatum strains atcc 10953, atcc 25586, f3, f6 and fev1. the proteins were purified by preparative gel electrophoresis. their behaviour in gel filtration and gel electrophoresis, their sensitivity to proteolytic enzymes, and their amino acid composition were investigated. the purified proteins were partly sequenced from the n-terminal end. a 3 ...19892636259
structural analysis of lipopolysaccharides from eikenella corrodens by use of murine monoclonal antibodies.the structure of lipopolysaccharides (lpss) of eikenella corrodens was analyzed with prepared murine monoclonal antibodies. a common core epitope was found in three of seven lps preparations from e. corrodens strains and fusobacterium nucleatum atcc 25586. four e. corrodens lpss were found to possess an o-side-chain epitope which cross-reacted with lpss from fusobacterium necrophorum atcc 25286 and capnocytophaga ochracea m-12. lipid a of e. corrodens lps shared an epitope common among lpss from ...19892643582
chemiluminescence of peripheral polymorphonuclear leukocytes from adult periodontitis patients.polymorphonuclear leukocytes (pmn's) constitute a primary host resistance factor against infection. this study investigated the chemiluminescent (cl) response of peripheral blood pmn's isolated from human subjects with adult periodontitis. 32 subjects were categorized on the basis of age and periodontal disease status into 4 equal groups--young healthy, young diseased, old healthy and old diseased. pmn cl was stimulated using heat-killed, serum-opsonized fusobacterium nucleatum--a specific perio ...19892646331
[oncogenes in human leukemia].results of our study on the activation of n-ras oncogene by point mutation in human leukemia and myelodysplastic syndrome have been described in this article. point mutation was observed mainly on the 12th, 13th and 61st amino acid codon of ras genes. therefore, oligomers containing mutations at these codons were used as probes for dot blot analysis of dna derived from patient's bone marrow cells or leukemia cells. polymerase chain reaction technique was used to amplify the dna of ras genes cont ...19892650633
enhancement of the infectivity of fusobacterium necrophorum by other bacteria.necrobacillosis is caused by fusobacterium necrophorum (fn), but other organisms are often present in the lesions. their possible role was studied in experiments made with a virulent fn strain which, by itself, produced fatal necrobacillosis in mice provided that large doses (greater than 10(6) organisms, subcutaneously) were given. mice were inoculated subcutaneously with fn suspended in sub-lethal doses (0.1 ml) of undiluted or diluted broth cultures of other bacteria. undiluted culture of a s ...19892661253
perspectives on the risks for occupational transmission of hiv-1 in the health-care workplace. 19892662448
molecular genetics of duchenne and becker muscular dystrophy: emphasis on improved diagnosis.duchenne muscular dystrophy (dmd) is a severe muscle-wasting disorder affecting 1 in 3500 males. a less severe and less common form is becker muscular dystrophy (bmd). only recently has the basic defect in dmd and bmd been recognized: a region on the human x chromosome is disrupted by mutation. the primary transcript of the region was detected, and cdna clones were isolated that encompassed the entire transcript. the sequence of the encoded protein was predicted from cdna nucleotide sequence, po ...19892663232
serum-free, chemically defined medium to evaluate the direct effects of growth factors and inhibitors on proliferation and function of neonatal rat cardiac muscle cells in culture.neonatal rat cardiac myocytes were isolated and cultured to evaluate the effects of growth factors and inhibitors on proliferation, survival, and functions in a serum-free medium. insulin and transferrin in mcdb 107 nutrient medium elicited dna and protein synthesis in cells on a fibronectin-coated culture surface in serum-free medium. insulin was most effective on both dna and protein synthesis in serum-free culture conditions. the serum-free, hormone-supplemented medium eliminated the contamin ...19892666383
serum-free, chemically defined medium to evaluate the direct effects of growth factors and inhibitors on proliferation and function of neonatal rat cardiac muscle cells in culture.neonatal rat cardiac myocytes were isolated and cultured to evaluate the effects of growth factors and inhibitors on proliferation, survival, and functions in a serum-free medium. insulin and transferrin in mcdb 107 nutrient medium elicited dna and protein synthesis in cells on a fibronectin-coated culture surface in serum-free medium. insulin was most effective on both dna and protein synthesis in serum-free culture conditions. the serum-free, hormone-supplemented medium eliminated the contamin ...19892666383
the precursor of beta-lactamase: purification, properties and folding kinetics.the precursor of escherichia coli rtem beta-lactamase was purified to homogeneity on a milligram scale by a procedure independent of the binding properties of the protein and refolded to an active, reduced form. for comparing the folding kinetics, the wild-type enzyme was reduced and a mutant was constructed, in which the two cysteines that form a very stable disulfide bond in the rtem enzyme were both changed into alanines. the rate of folding was determined by directly measuring the increase i ...19892670555
report of the committee on the genetic constitution of chromosome 3. 19892676365
modulation of human neutrophil adherence by periodontopathic bacteria: reversal by specific monoclonal antibodies.previous investigations by us have shown that direct interaction of fusobacterium nucleatum with polymorphonuclear neutrophils (pmns) results in the stimulation of pmn adherence whereas direct interaction with bacteroides gingivalis results in pmn suppression. in the present study, panels of monoclonal antibodies (mabs) raised against cell wall antigens of f. nucleatum and b. gingivalis were tested to determine their ability to block the modulatory effects of the bacteria in their interactions w ...19892680992
remodeling of the rat small intestinal mucosa during the suckling period.the development of the suckling rat small intestine has been reexamined to investigate changes to cell kinetics and morphology more fully. in this period of rapid growth and reorganization, villi and crypts steadily increase in size in proximal regions; however, in distal regions a dramatic shortening of villi occurs at about 15 days. the result of this remodeling is that the normal adult proximodistal gradient of villus height is established at 15 days, migration rates in both regions increase ...19892681650
"reverse" dna hybridization method for the rapid identification of subgingival microorganisms.a "reverse" hybridization method is described, in which whole chromosomal dna was extracted from 10-20 colonies of "unknown" strains in pure culture and labelled with digoxigenin by a random primer technique. dna probes were prepared from a total of 23 strains and hybridized with targets containing 100 ng purified, denatured dna from 38 reference strains fixed to nitrocellulose. 21/23 digoxigenin-labelled dna probes successfully detected all members of the homologous species present on filters. ...19892639298
[bacterial invasion in periodontitis, is it important in periodontal treatment?].during the progression of periodontal disease bacteria invade the epithelial lining of the gingival pocket and the underlying connective tissue. this invasion was demonstrated in acute necrotizing ulcerative gingivitis (anug), localized juvenile periodontitis (ljp), rapidly progressive periodontitis in adults (rpp) and in children with papillon-lefèvre syndrome associated periodontitis. using morphologic criteria or fluorescent antibody staining techniques spirochetes, actinobacillus actinomycet ...19892685948
myosin light chain phosphorylation in 32p-labeled rabbit aorta stimulated by phorbol 12,13-dibutyrate and phenylephrine.the mechanism(s) of force development in vascular smooth muscle following pharmacological activation of protein kinase c by phorbol esters are not known. in this study, we examined the myosin light chain phosphorylation response following stimulation by phorbol 12,13-dibutyrate (pdb) or phenylephrine in rabbit aorta which had been incubated with 32po4 in order to label atp pools. through tryptic phosphopeptide mapping of myosin light chain from intact tissue and comparison to controls using puri ...19892687275
[insulin and hypertension--the mechanisms of high blood pressure during chronic insulin infusion].the purpose of this study was to assess the effects of chronic insulin infusion on blood pressure and urinary sodium excretion in wistar rats. fifteen male wistar rats weighing about 220 g were used. the rats were housed in metabolic cage and measured urine volume. osmotic minipumps filled with insulin (0.57 u/day, insulin group, n = 9) or saline (0.014 cc/day, control group, n = 6) were implanted subcutaneously under ether anaesthesia, and blood pressure, urine volume, urinary sodium excretion ...19892687519
assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin.a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ...19892688936
the efficiency of interaction of deoxyribonucleoside-5'-mono-, di- and triphosphates with the active centre of e. coli dna polymerase i klenow fragment.the interaction of deoxyribonucleoside-5'-mono-, di- and triphosphates with e. coli dna polymerase i klenow fragments was examined. dissociation constants of the enzyme complex with nucleotides were determined from the data on the enzyme inactivation by adenosine 2',3'-riboepoxide 5'-triphosphate. the role of nucleotide bases, phosphate groups and sugar moieties in the complex formation of nucleotides with the enzyme was elucidated. the necessity of complementary interaction of nucleotides with ...19892689231
falciparum malaria--present day problems. an experience with 425 cases.clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...19892693436
the microbiology of hiv-associated periodontal lesions.2 intraoral lesions associated with human immunodeficiency virus (hiv) infection have recently been described: an atypical gingivitis and a rapidly progressive periodontitis. the microbiota associated with these gingival and periodontal lesions was investigated. subgingival plaque samples were taken from 45 hiv-seropositive homosexual men and from 44 hiv-seronegative control subjects. each sampled site was clinically and radiographically classified as hiv-associated gingivitis, hiv-associated pe ...19892693496
[characterization of monoclonal antibodies to toxoplasma gondii].proteins of toxoplasma lysate were separated by sds-polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. the range of protein bands was 6-100 kd. monoclonal antibodies 1b2, 5b10, 4f6 reacted with antigens of 21 kd, 35 kd and 66 kd. monoclonal antibodies reacted with major antigens of the rh-tachyzoiten surface and hanr-cysts.19892694652
[proposal for a new therapeutic strategy in high genital infections].78 cases of acute salpingitis were treated with the association oflocet-augmentin in a multicenter study. an accurate clinical and bacteriological evaluation was carried out prior to the treatment and a control laparoscopy was performed in 31 cases. a chlamydia infection was responsible in 56 p. cent of the cases; only one bacteria was identified in 56 cases and an association of two or three bacteria in 15 cases; in 7 cases, no bacteria was identified. the antibiotic association was effective i ...19892696066
[microbiological and serological investigation of periodontal disease activity].if disease activity in periodontal disease can be determined, the therapeutic measures of periodontitis may become diverse and different from patient to patient. the purpose of the present study was to evaluate the usefulness of microbiological and serological examination of periodontopathic bacteria in detecting destructive periodontal disease activity. one hundred and forty-eight sites in 52 subjects with moderate to severe periodontitis were studied clinically. clinical parameters included pl ...19892700196
[microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy].the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ...19892700361
design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum).the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ...19892708344
design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum).the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ...19892708344
structure-function relationships in rat brainstem subnucleus interpolaris: v. functional consequences of neonatal infraorbital nerve section.in a prior study (jacquin et al., '86c), the response properties and projections of neonatally axotomized trigeminal (v) primary afferents were studied in the adult rat. here, single-unit recording, electrical stimulation, and receptive field (rf) mapping techniques were also used to assess the functional consequences of neonatal infraorbital nerve section upon postsynaptic cells in v brainstem subnucleus interpolaris (spvi). of 904 cells studied, 385 were from normal adults and 519 were from ne ...19892708594
effects of periodontopathic bacteria on il-1 and il-1 inhibitor production by human polymorphonuclear neutrophils.the effect of heat-killed periodontopathic bacteria on the production of interleukin-1 (il-1) and an il-1 inhibitor by human polymorphonuclear neutrophils (pmn's) was examined. peripheral blood was obtained from 18 healthy volunteers and the pmn's were separated using dextran sedimentation and ficoll-paque density gradient centrifugation. the pmn's (5 x 10(5) cells/well) were cultured in serum-free media with or without heat-killed periodontopathic bacteria. four gram-negative periodontopathic b ...19892640313
[studies on anaerobic infection in oro-maxillary region--rapid diagnosis by gas-liquid chromatography and antibiotic susceptibilities of anaerobic bacteria].subject material for this study was pus collected from patients with purulent inflammation in the oro-maxillary region. direct gas-liquid chromatography (glc) analysis was made, bacterial isolation and identification were carried out, and comparisons were made with results from glc analysis and anaerobic isolates in a pyg medium. in addition, antibiotic susceptibilities of anaerobic bacteria were examined. results 1. anaerobic bacteria were isolated from 85 of 100 cases of obstructive abscesses. ...19892641196
specific petit mal anticonvulsants reduce calcium currents in thalamic neurons.low-threshold calcium current (ltcc) in thalamic neurons is important in generation of normal thalamocortical rhythms, and may be involved in the genesis of abnormal activities such as spike-wave discharges that characterize petit mal epilepsy. ethosuximide and dimethadione, anticonvulsants effective in petit mal, reduced the ltcc when applied to thalamic neurons at clinically relevant concentrations. therapeutic concentrations of phenytoin and carbamazepine, drugs ineffective in the control of ...19892710401
the projection pattern of forepaw nerves to the cuneate nucleus of the raccoon.the transganglionic transport of horseradish peroxidase (hrp) was used to determine the projection pattern within the cuneate nucleus of the 4 major nerves innervating the forepaw of the raccoon, a carnivore noted for its tactile and manipulative abilities. the two nerves innervating the dorsal, hairy skin and claws (the radial and dorsal ulnar nerves) projected to the marginal rim of the cuneate nucleus, but not to the middle cluster region or to the caudal region of the nucleus. the two nerves ...19892710406
the projection pattern of forepaw nerves to the cuneate nucleus of the raccoon.the transganglionic transport of horseradish peroxidase (hrp) was used to determine the projection pattern within the cuneate nucleus of the 4 major nerves innervating the forepaw of the raccoon, a carnivore noted for its tactile and manipulative abilities. the two nerves innervating the dorsal, hairy skin and claws (the radial and dorsal ulnar nerves) projected to the marginal rim of the cuneate nucleus, but not to the middle cluster region or to the caudal region of the nucleus. the two nerves ...19892710406
[spondylodiscitis caused by fusobacterium nucleatum. apropos of 1 case]. 19892711113
quantitative bacteriology of a case of acute parotitis.it is generally assumed that the microorganisms responsible for acute suppurative parotitis are facultative anaerobes, primarily staphylococcus aureus and streptococcus viridans. a quantitative microbiologic investigation of an aspirate of pus from the case we report revealed a microbial flora (5.1 x 10(6) cfu/ml) consisting entirely of strict anaerobes (fusobacterium nucleatum and peptostreptococcus anaerobius). the strain of f. nucleatum was sensitive to metronidazole but resistant to penicill ...19892682427
an evaluation of the multiple-breath nitrogen washout as a pulmonary function test in dairy cattle.multiple-breath nitrogen washouts (mbnw) were performed with 21 adult dairy cows (seven normal cows tested on two days each, four clinically normal cows tested on six days, and ten cows with respiratory disease tested on one day each). the functional residual capacity (frc), lung clearance index (lci), becklake index (bi), mixing ratio (mr), pulmonary n2 clearance delay (pcd), index of distribution of inspiration (idi) and ventilatory efficiency (eff) were calculated for each mbnw. the lci, mr, ...19892713778
an evaluation of the multiple-breath nitrogen washout as a pulmonary function test in dairy cattle.multiple-breath nitrogen washouts (mbnw) were performed with 21 adult dairy cows (seven normal cows tested on two days each, four clinically normal cows tested on six days, and ten cows with respiratory disease tested on one day each). the functional residual capacity (frc), lung clearance index (lci), becklake index (bi), mixing ratio (mr), pulmonary n2 clearance delay (pcd), index of distribution of inspiration (idi) and ventilatory efficiency (eff) were calculated for each mbnw. the lci, mr, ...19892713778
splenic abscess from fusobacterium nucleatum. 19892719435
clonazepam in the treatment of obsessive compulsive disorder associated with panic disorder in one patient.the authors present the case of a 21-year-old man with obsessive compulsive disorder (ocd) complicated by panic disorder, whose ocd and panic symptoms resolved during clonazepam treatment. prior treatment with an equivalent dose of lorazepam had ameliorated his panic attacks without affecting his obsessions or compulsions. clonazepam worked with a rapidity and completeness uncharacteristic of more established treatments for ocd.19892738031
fetal cortical transplants reduce the thalamic atrophy induced by frontal cortical lesions in newborn rats.fetal neocortical tissue was grafted into frontal cortex lesion cavities made in newborn rats. after survival periods extending up to 14 months, volumetric measurements of the total thalamus and of the lateral, medial and anterior thalamic compartments showed an amelioration of the thalamic atrophy that normally is found after cortical lesions. these results correspond to previous findings demonstrating interconnections between fetal cortical transplants and the host thalamus.19892710397
effects of growth factors on cytosolic free calcium concentration and dna synthesis in cultured rat aortic smooth muscle cells.proliferation of vascular smooth muscle cells (smcs) has been considered to be an important process in the development of atherosclerosis. this study was conducted to investigate the role of cytosolic free calcium in dna synthesis of smcs stimulated by growth factors. platelet-derived growth factor (pdgf), epidermal growth factor (egf) and somatomedin-c (sm-c) increased [3h]thymidine incorporation, an index of dna synthesis, and cell number of rat aortic smcs after 36 hr of incubation. cytosolic ...19892741168
identification by c-banding of two human prostate tumour cell lines, 1013l and du 145.two human prostate carcinoma cell lines are easily distinguished by c-banding. du 145 has one metacentric chromosome with a large centromeric band, 1 metacentric and 2 acrocentric chromosomes with interstitial c-bands. the y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in 1013l only one metacentric chromosome contained distinct extra c bands--one at the centromere and one interstitially. the y chromosome was present in 72% of the cells at passa ...19892744886
macrophage subpopulations in lamina propria of normal and inflamed colon and terminal ileum.the aim of this study was to characterise human intestinal macrophages in normal and inflamed ileum and colon. immunoperoxidase staining with a panel of monoclonal antibodies and histochemical staining for acid phosphatase and non-specific esterase was performed. in the superficial lamina propria, normal colonic macrophages were larger and more strongly positive for acid phosphatase and non-specific esterase than those in normal terminal ileum. there were more macrophages staining with monoclona ...19892753407
the effect of subminimal inhibitory concentrations of antimicrobial agents on three bacterial mixtures.a test tube technique was developed to screen bacterial mixtures to detect interbacterial interactions that play a role in determining sensitivity to antimicrobial agents. we found 3 mixtures where these bacterial interactions change the sensitivity to antimicrobials or change the proportions of each bacterial species in the mixture. the mixtures were: fusobacterium nucleatum 102.3 and bacteroides endodontalis atcc 35406; f. nucleatum 102.3 and b. endodontalis bn11 a-f; and capnocytophaga ochrac ...19892762019
isolation of fusobacterium nucleatum and electron microscopic observations of spirochetes from tropical skin ulcers in papua new guinea.tropical ulcer is a disabling condition of the lower leg affecting mainly young adults and older children. microscopic observations of lesion material have shown fusiform bacilli and spirochetes. we used anaerobic culture techniques to isolate and identify these fusiform bacilli. electron microscopic (em) studies were performed to characterize the spirochetes. material collected on swabs was used to inoculate pre-reduced media and to prepare smears for gram staining; the swabs were placed in fix ...19892712197
fetal cortical transplants reduce the thalamic atrophy induced by frontal cortical lesions in newborn rats.fetal neocortical tissue was grafted into frontal cortex lesion cavities made in newborn rats. after survival periods extending up to 14 months, volumetric measurements of the total thalamus and of the lateral, medial and anterior thalamic compartments showed an amelioration of the thalamic atrophy that normally is found after cortical lesions. these results correspond to previous findings demonstrating interconnections between fetal cortical transplants and the host thalamus.19892710397
the microbiota of early periodontitis lesions in adults.the predominant cultivable microbiota of shallow progressive (active) periodontal lesions in 4 adults (29-40 years) was compared to that of shallow inactive sites. periodontal attachment levels were monitored longitudinally, and sites showing 2-3 mm attachment loss in the previous 1-2 months were considered disease active. inactive sites showed no change in attachment level. the microbiota of shallow sites was dominated by gram negative rod species including wolinella recta, bacteroides forsythu ...19892768541
accuracy of the subrenal capsule xenograft assay in predicting the clinical growth and chemosensitivity of human squamous head and neck carcinomas.thirty-seven of 54 human squamous head and neck carcinomas were successfully grown as first transplant generation xenografts under the kidney capsule of conventional mice immunosuppressed by daily treatment with 60 mg/kg of cyclosporine. of the 18 different tumors evaluated for chemosensitivity, 39% responded to cis-platinum, 19% to 5-fluorouracil, 33% to methotrexate, and 40% to cis-pt/5fu. in comparing the assay results in nine patients who received the same chemotherapeutic drugs, two of thre ...19892770306
[excretion of phenoltetrachlorophthalein in the bile in rats].phenoltetrachlorphthalein secretion into bile was followed in wistar strain rats intoxicated per os by tetrachlormethane (0.25, 0.50, 2.00, and 3.00 ml/kg) after 1, 2, 4 and 8 days after intoxication. biotransformation processes resulting in the formation of phenoltetrachlorphthalein metabolites are altered during the intoxication unequally. so far mutual relation of secreted metabolites has not been proved to serve generally as a simple criterion for absolute activity assessment of hepatic gluc ...19892772547
lyme disease concurrent with human immunodeficiency virus infection. 19892773968
a comparison of the effects of morphine on 5-ht metabolism in the periaqueductal gray, ventromedial medulla and medullary dorsal horn: in vivo electrochemical studies in freely moving rats.the effect of systemic morphine on serotonin (5-ht) metabolism within the dorsal raphe nucleus (drn) has been investigated by in vivo 5-hydroxyindole electrochemical (peak '3') detection in freely moving rats. morphine caused a weak and delayed, but naloxone-reversible, increase in peak '3'. this increase was poorly, if at all, correlated with the morphine-induced analgesia. finally, stress and/or noxious stimulation had no effect on this signal. these results are compared with our previous stud ...19892776031
coaggregation of fusobacterium nucleatum, selenomonas flueggei, selenomonas infelix, selenomonas noxia, and selenomonas sputigena with strains from 11 genera of oral bacteria.twenty-eight strains of fusobacterium nucleatum and 41 selenomonas strains, including s. sputigena (24 strains), s. flueggei (10 strains), s. infelix (5 strains), and s. noxia (2 strains), were tested for their ability to coaggregate with each other and with 49 other strains of oral bacteria representing actinobacillus, actinomyces, bacteroides, capnocytophaga, gemella, peptostreptococcus, porphyromonas, propionibacterium, rothia, streptococcus, and veillonella species. selenomonads coaggregated ...19892777378
inhibition of coaggregation between fusobacterium nucleatum and porphyromonas (bacteroides) gingivalis by lactose and related sugars.the coaggregation of fusobacterium nucleatum pk1594 and porphyromonas (bacteroides) gingivalis pk1924 was inhibited equally well by lactose, n-acetyl-d-galactosamine, and d-galactose, which caused 50% inhibition of coaggregation at 2 mm sugar concentration. other sugars such as d-galactosamine, d-fucose (6-deoxy-d-galactose), and alpha-methyl- and beta-methyl-d-galactosides also inhibited coaggregation. sugar specificity was apparent, since neither l-fucose, l-rhamnose, n-acetyl-d-glucosamine, n ...19892777379
direct measurement of basolateral membrane potentials from cells of the macula densa.at the present time, little is known concerning the electrophysiology of the cells of the macula densa and whether or not these cells are electrically responsive to alterations in luminal fluid composition. to investigate this issue, cortical thick ascending limbs (ctal) containing macula densa and attached glomeruli were dissected from rabbit kidney and the ctal perfused in vitro. basolateral membrane potential (vbl) was measured with microelectrodes in macula densa cells and, for comparison, i ...19892782426
[septicemia caused by beta-lactamase producing fusobacterium nucleatum in a patient with neutropenia]. 19892788381
effects of growth factors on cytosolic free calcium concentration and dna synthesis in cultured rat aortic smooth muscle cells.proliferation of vascular smooth muscle cells (smcs) has been considered to be an important process in the development of atherosclerosis. this study was conducted to investigate the role of cytosolic free calcium in dna synthesis of smcs stimulated by growth factors. platelet-derived growth factor (pdgf), epidermal growth factor (egf) and somatomedin-c (sm-c) increased [3h]thymidine incorporation, an index of dna synthesis, and cell number of rat aortic smcs after 36 hr of incubation. cytosolic ...19892741168
a t-cell-specific transcriptional enhancer element 3' of c alpha in the human t-cell receptor alpha locus.a transcriptional enhancer element has been identified 4.5 kilobases 3' of c alpha (constant region alpha chain) in the human t-cell receptor (tcr) alpha-chain locus. this enhancer is active on both a tcr v alpha (variable region alpha chain) promoter and the minimal simian virus 40 promotor in tcr alpha/beta jurkat and el4 cells but is inactive on a v alpha promoter in human tcr gamma/delta peer and molt-13 cells, clone 13 b cells, and hela fibroblasts. the enhancer has been localized to a 116- ...19892788889
[legal foundation and practical execution of slaughtering and meat examination of game animals].meat regulations also involve game inspection. on the one hand the previous publications dealt with the regulations and their practice concerning game intended for slaughter, especially the control of fences and the gaining of meat. on the other hand regulations concerning game hunted for food (deer: red deer, sika deer, fallow deer, roe deer; horned ungulates: european mufflon, chamois; wild boar; european hare; european rabbit; game such as badger and raccoon) and regulations to be observed by ...19892763282
influence of age and immunization on development of gingivitis in rats.to study the effect of age and antigenic priming on the development of gingivitis, 33 healthy rats were placed in contact with streptococcus mutans, actinomyces viscosus, fusobacterium nucleatum, and bacteroides gingivalis. on days 0, 3, 7, and 14 after inoculation, the gingival condition was judged clinically and histologically, and serum antibody titers against the bacteria were measured. the rats were divided into three groups: 1 month old, 3 months old, and 3 months old immunized. none of th ...19892789463
modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats.the state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (ki) for the circulating tracer [14c]alpha-aminoisobutyric acid. pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral gluc ...19892812289
modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats.the state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (ki) for the circulating tracer [14c]alpha-aminoisobutyric acid. pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral gluc ...19892812289
lack of correlation of maternal human immunodeficiency virus infection with neonatal malformations.a malformation syndrome has been proposed in infants with acquired immunodeficiency syndrome or acquired immunodeficiency syndrome-related complex secondary to congenital infection with human immunodeficiency virus (hiv) in the united states and europe. to determine whether embryopathy is detectable in hiv-exposed african infants, 85 infants of hiv-seropositive mothers and 98 infants of hiv-seronegative mothers in nairobi, kenya, were examined for minor and major anomalous features shortly after ...19892812915
disease response to tobacco mosaic virus in transgenic tobacco plants that constitutively express the pathogenesis-related pr1b gene.correlation of the temporal and spacial pattern of induction of the pathogenesis-related (pr) genes pr1a, pr1b, and pr1c with viral infections in certain tobacco cultivars has implicated pr proteins in viral disease resistance. to test whether the pr1 proteins of tobacco are involved in viral resistance, transgenic nicotiana tabacum plants were constructed which constitutively express the pr1b gene. this protein was secreted from cells of transgenic plants and accumulated in the extracellular sp ...19892815592
Displaying items 401 - 500 of 4852