PMID(sorted descending)
increased proliferation and decreased membrane permeability as defense mechanisms of fusobacterium nucleatum against human neutrophilic peptide-1.human neutrophilic peptides (hnps) constitute a class of host defense molecules, which contribute to the non-oxidative killing of bacteria and other microorganisms. since the adaptability is crucial to bacterial survival in changing environments, it is of interest to know how fusobacterium nucleatum, the major bridge organism connecting early and late colonizers in dental biofilms, defends itself against hnps. this study aimed to examine the planktonic growth, membrane permeability, and biofilm ...201425132418
individual growth detection of bacterial species in an in vitro oral polymicrobial biofilm model.most in vitro studies on the antibacterial effects of antiseptics have used planktonic bacteria in monocultures. however, this study design does not reflect the in vivo situation in oral cavities harboring different bacterial species that live in symbiotic relationships in biofilms. the aim of this study was to establish a simple in vitro polymicrobial model consisting of only three bacterial strains of different phases of oral biofilm formation to simulate in vivo oral conditions. therefore, we ...201425119373
an in vitro biofilm model associated to dental implants: structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces.the impact of implant surfaces in dental biofilm development is presently unknown. the aim of this investigation was to assess in vitro the development of a complex biofilm model on titanium and zirconium implant surfaces, and to compare it with the same biofilm formed on hydroxyapatite surface.201425110288
axenic culture of a candidate division tm7 bacterium from the human oral cavity and biofilm interactions with other oral bacteria.the diversity of bacterial species in the human oral cavity is well recognized, but a high proportion of them are presently uncultivable. candidate division tm7 bacteria are almost always detected in metagenomic studies but have not yet been cultivated. in this paper, we identified candidate division tm7 bacterial phylotypes in mature plaque samples from around orthodontic bonds in subjects undergoing orthodontic treatment. successive rounds of enrichment in laboratory media led to the isolation ...201425107981
fusobacterium detected in colonic biopsy and clinicopathological features of ulcerative colitis in japan.fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. here, we performed prevalence screening of fusobacterium in ulcerative colitis (uc) in japanese patients.201525102986
effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults.oral bacteria live in symbiosis with the host. therefore, when mouthwashes are indicated, selective inhibition of taxa contributing to disease is preferred instead of broad-spectrum antimicrobials. the potential selectivity of an oxygenating mouthwash, ardox-x® (ax), has not been assessed. the aim of this study was to determine the antimicrobial potential of ax and the effects of a twice-daily oral rinse on dental plaque composition.201425101249
reducing the bioactivity of tannerella forsythia lipopolysaccharide by porphyromonas gingivalis.tannerella forsythia is considered a pathogen of periodontitis and forms a biofilm with multi-species bacteria in oral cavity. lipopolysaccharide is a powerful immunostimulator and induces inflammation and shock. the purpose of this study was to investigate the characteristics of t. forsythia lps in its co-cultivation with fusobacterium nucleatum or porphyromonas gingivalis. t. forsythia was co-cultured in the presence and absence of f. nucleatum and p. gingivalis and then t. forsythia lps was e ...201425098564
a comparative antimicrobial analysis of various root canal irrigating solutions on endodontic pathogens: an in vitro study.evolution in understanding the poly-microbial environment of both endodontic infections and that of failed root canal treatments has been debatable over the years. the present study was designed to compare and analyze the effect of various root canal irrigation solutions on certain endodontic pathogens in vitro.201425095835
microbial community in persistent apical periodontitis: a 16s rrna gene clone library characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach.201525088120
active invasion of porphyromonas gingivalis and infection-induced complement activation in apoe-/- mice brains.periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of alzheimer's disease. apoe-/- mice were orally infected (n = 12) with porphyromonas gingivalis, treponema denticola, tannerella forsythia, and fusobacterium nucleatum as mono- and polymicrobial infections. apoe-/- mice were sacrificed following 12 and 24 weeks of chronic infection. bacterial gen ...201525061055
high-level antimicrobial efficacy of representative mediterranean natural plant extracts against oral microorganisms.nature is an unexplored reservoir of novel phytopharmaceuticals. since biofilm-related oral diseases often correlate with antibiotic resistance, plant-derived antimicrobial agents could enhance existing treatment options. therefore, the rationale of the present report was to examine the antimicrobial impact of mediterranean natural extracts on oral microorganisms. five different extracts from olea europaea, mastic gum, and inula viscosa were tested against ten bacteria and one candida albicans s ...201425054150
pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils.endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. to investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections (endodontic pathogens [ep]), i.e., fusobacterium nucleatum, streptococcus intermedius, parvimonas micra, and prevotella intermedia, was inoculated into subcutaneousl ...201425024367
susceptibility of methacrylate-based root canal filling to degradation by bacteria found in endodontic present a case of endodontic failure obturated with a methacrylate-based root filling material, resilon/realseal (rs). to determine if rs is susceptible to biodegradation by endodontically relevant microbes by a method known to show rs degradation.201425019109
bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site.sialic acids are a family of related nine-carbon sugar acids that play important roles in both eukaryotes and prokaryotes. these sialic acids are incorporated/decorated onto lipooligosaccharides as terminal sugars in multiple bacteria to evade the host immune system. many pathogenic bacteria scavenge sialic acids from their host and use them for molecular mimicry. the first step of this process is the transport of sialic acid to the cytoplasm, which often takes place using a tripartite atp-indep ...201425004958
biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial-stimulated pdl cells.the present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (pdl) cells in the presence of bacterial challenge. cells were seeded on bioflex culture plates and exposed to fusobacterium nucleatum atcc 25586 and/or cyclic tensile strain (cts) of low (ctsl) and high (ctsh) magnitudes for 1 and 3 days. synthesis of cyclooxygenase-2 (cox2) and prostaglandin e2 (pge2) was evaluated by elisa. gene ...201424976684
fusobacterium and enterobacteriaceae: important players for crc?the gut microbiota plays an essential role in regulating intestinal homeostasis through its capacity to modulate various biological activities ranging from barrier, immunity and metabolic function. not surprisingly, microbial dysbiosis is associated with numerous intestinal disorders including inflammatory bowel diseases (ibd) and colorectal cancer (crc). in this piece, we will review recent evidence that gut microbial dysbiosis can influence intestinal disease, including colitis and crc. we wil ...201424972311
identification of occult fusobacterium nucleatum central nervous system infection by use of pcr-electrospray ionization mass spectrometry.anaerobic bacteria are often difficult to detect, especially after the initiation of antibiotics. we describe the application of pcr-electrospray ionization mass spectrometry (pcr/esi-ms) using a sample of cerebrospinal fluid to identify an anaerobic gram-negative bacillus, fusobacterium nucleatum, in a patient with "culture-negative" meningitis and cerebral abscesses.201424966358
colonisation of gingival epithelia by subgingival biofilms in vitro: role of "red complex" bacteria.biofilm formation on tooth surface results in colonisation and invasion of the juxtaposed gingival tissue, eliciting strong inflammatory responses that lead to periodontal disease. this in vitro study investigated the colonisation of human gingival multi-layered epithelium by multi-species subgingival biofilms, and evaluated the relative effects of the "red complex" species (porphyromonas gingivalis, tannerella forsythia and treponema denticola).201424949828
the influence of orthodontic fixed appliances on the oral microbiota: a systematic investigate whether there is scientific evidence to support the hypothesis that the presence of orthodontic fixed appliances influences the oral microbiota.201424945514
gastrointestinal variant of lemierre syndrome: fusobacterium nucleatum bacteremia-associated hepatic vein thrombosis: a case report and literature review.fusobacterium nucleatum is a gram-negative bacillius commonly found in oropharynx and is traditionally associated with lemierre syndrome, which is characterized by history of recent oropharyngeal infection, internal jugular vein thrombosis, and isolation of anaerobic pathogens, mainly fuosobacterium necrophorum. however, recent evidence indicated that f. nucleatum is also a normal resident of human gut. less than a dozen of case reports had linked f. nucleatum to gastrointestinal variant of lemi ...201724942004
in vitro efficacy of cefovecin against anaerobic bacteria isolated from subgingival plaque of dogs and cats with periodontal disease.periodontal disease is a common disease of dogs and cats often requiring antimicrobial treatment as an adjunct to mechanical debridement. however, correct compliance with oral antimicrobial therapy in companion animals is often difficult. cefovecin is a recently introduced veterinary cephalosporin that has demonstrated prolonged concentrations in extracellular fluid, allowing for dosing intervals of up to 14 days. subgingival samples were collected from the oral cavity of 29 dogs and eight cats ...201424930431
effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria.little is known about the effects of stress hormones on the etiologic agents of halitosis. thus, the aim of this study was to evaluate in vitro the effects of adrenaline (adr), noradrenaline (na) and cortisol (cort) on bacteria that produce volatile sulfur compounds (vsc), the major gases responsible for bad breath. cultures of fusobacterium nucleatum (fn), porphyromonas endodontalis (pe), prevotella intermedia (pi) and porphyromonas gingivalis (pg) were exposed to 50 µm adr, na and cort or equi ...201424918364
rapid brain death caused by a cerebellar abscess with fusobacterium nucleatum in a young man with drug abuse: a case report.fusobacterium nucleatum is a strict anaerobic microorganism that causes disease entities such as periodontal and soft tissue abscesses, pulmonary and intraabdominal infections and very rarely intracerebral infections.201424915846
the interaction of implant luting cements and oral bacteria linked to peri-implant disease: an in vitro analysis of planktonic and biofilm growth--a preliminary study.there is little consensus on the most appropriate cement to use when restoring a cement-retained, implant-supported restoration. one consideration should be the interaction of pathogenic oral bacteria with restorative cements.201524909337
ubiquitous sialometabolism present among oral fusobacteria.fusobacterium nucleatum is a ubiquitous member of the human oral flora and is associated with the development of periodontitis and a variety of other types of polymicrobial infections of the mucosa. in the oral cavity, this species is one of the few that is prevalent in both healthy and diseased subgingival plaque. using microarray analysis, we examined the transcriptional response of f. nucleatum subspecies nucleatum to whole blood in order to identify some of the genetic responses that might o ...201424896093
the antibiofilm activity of lingonberry flavonoids against oral pathogens is a case connected to residual complexity.the antimicrobial activity of lingonberry (vaccinium vitis-idaea l.) was evaluated against two oral pathogens, streptococcus mutans and fusobacterium nucleatum. long-bed gel permeation chromatography (gpc; sephadex lh-20) yielded purified flavonoids, with the most efficient minimum inhibitory concentrations (mics) against planktonic cells in the anthocyanin and procyanidin primary fractions against f. nucleatum (63-125 μg/ml) and in the procyanidin rich fraction against s. mutans (16-31 μg/ml). ...201424879903
the kampo medicine rokumigan possesses antibiofilm, anti-inflammatory, and wound healing properties.periodontal diseases, which are inflammatory diseases of bacterial origin affecting the tooth-supporting tissues, are characterized by inflammation and destruction of gingival connective tissue and alveolar bone, and may lead to tooth loss. the aim of the study was to investigate rokumigan, a kampo japanese traditional medicine made of six different plants, for its capacity to prevent biofilm formation by fusobacterium nucleatum, to inhibit interleukin-6 (il-6) and interleukin-8 (il-8) secretion ...201424877093
microbial evaluation of traumatized teeth treated with triple antibiotic paste or calcium hydroxide with 2% chlorhexidine gel in pulp revascularization.revascularization outcome depends on microbial elimination because apical repair will not happen in the presence of infected tissues. this study evaluated the microbial composition of traumatized immature teeth and assessed their reduction during different stages of the revascularization procedures performed with 2 intracanal medicaments.201424862703
internal bacterial colonization of implants: association with peri-implant bone loss.the aim of the present longitudinal study was to investigate bacterial colonization of the internal implant cavity and to evaluate a possible association with peri-implant bone loss.201524861845
clinical and microbiological characterization of localized aggressive periodontitis: a cohort study.localized aggressive periodontitis (lagp) is an infectious periodontal disease which generally affects young people. recent data suggest the involvement of different bacterial species in different populations. the causative bacterial species in israel has never been identified despite a high prevalence of lagp in this population. the objectives of this study were to characterize the bacterial microbiota of periodontal pockets within an israeli lagp population who were also clinically assessed.201424861390
intestinal dysbiosis: novel mechanisms by which gut microbes trigger and prevent research has identified specific intestinal colonizing microbes that can have significant influence on health and disease. evidence is reviewed supporting an association between fusobacterium nucleatum and colon cancer and for a protective role of faecalibacterium prausnitzii in inflammatory bowel disease, of escherichia coli nissle 1917 in acute intestinal inflammation, of bifidobacterium infantis in neonatal necrotizing enterocolitis, and of akkermansia muciniphila in obesity and diabetes. ...201424857830
evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time pcr.the aim of this study was to investigate the mandibular third molar pericoronitis flora by using real-time polymerase chain reaction (pcr).201424835305
subgingival bacteria in ghanaian adolescents with or without progression of attachment loss.this study describes subgingival bacterial profiles associated with clinical periodontal status in ghanaian adolescents with or without progression of attachment loss.201424834145
vertebral osteomyelitis caused by fusobacterium nucleatum: a case report.a 58-year-old man with a history of chronic alcohol abuse presented with vertebral osteomyelitis caused by fusobacterium nucleatum. this case demonstrated the foul smelling abscess characteristic of anaerobic infection. deep anaerobic infections are well known to be resistant to closed treatment, and the computerized tomographic scan confirmed the diagnosis of a paravertebral abscess in this case. an increased awareness among clinicians that anaerobic bacilli can cause oteomyelitis, together wit ...197924823152
relationship between expression of human gingival beta-defensins and levels of periodontopathogens in subgingival plaque.human beta-defensins (hbds) are a group of antimicrobial peptides important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. the aim of this study was to explore relationships among hbds, total subgingival bacteria and periodontopathogens in healthy subjects and in patients with chronic periodontitis.201524814979
microbial mucosal colonic shifts associated with the development of colorectal cancer reveal the presence of different bacterial and archaeal biomarkers.epidemiological studies demonstrate a link between gastrointestinal cancers and environmental factors such as diet. it has been suggested that environmental cancer risk is determined by the interaction between diet and microbes. thus, the purpose of this study was to examine the hypothesis that microbiota composition during colorectal cancer (crc) progression might differ depending on the stage of the disease.201524811328
a periodontitis-associated multispecies model of an oral biofilm.while single-species biofilms have been studied extensively, we know notably little regarding multispecies biofilms and their interactions. the purpose of this study was to develop and evaluate an in vitro multispecies dental biofilm model that aimed to mimic the environment of chronic periodontitis.201424778902
red wine and oenological extracts display antimicrobial effects in an oral bacteria biofilm model.the antimicrobial effects of red wine and its inherent components on oral microbiota were studied by using a 5-species biofilm model of the supragingival plaque that includes actinomyces oris, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar. microbiological analysis (cfu counting and confocal laser scanning microscopy) of the biofilms after the application of red wine, dealcoholized red wine, and red wine extract solutions spiked or not with grape seed ...201424773294
in vitro evaluation of a multispecies oral biofilm on different implant surfaces.biofilm accumulation on implant surfaces is one of the most important factors for early and late implant failure. because of the related clinical implications, the aim of this in vitro study was to compare the bacterial cell attachment of a four-species oral biofilm on titanium discs of purity grade 2 and 4, with machined surfaces and etched-thermochemically modified with avantblast®. the in vitro biofilm model was composed of early (actinomyces naeslundii, streptococcus gordonii), secondary (ve ...201424770899
salivary pellicle composition and multispecies biofilm developed on titanium nitrided by cold plasma.the aim of this study was to evaluate the composition of the salivary pellicle (sp) and multispecies biofilm developed on titanium nitrided by cold plasma.201424769315
new bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings.the aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings.201424767562
antibacterial action of chlorhexidine/thymol containing varnishes in vitro and in vivo.the antibacterial activity of two different formulations of a chlorhexidine/thymol varnish should be elucidated in vitro and in vivo.201424738759
periodontal disease, atherosclerosis, adverse pregnancy outcomes, and head-and-neck cancer.interrelationships between periodontal infection and systemic conditions such as cardiovascular disease, adverse pregnancy outcomes, and head-and-neck cancer have become increasingly appreciated in recent years. periodontitis is associated with cardiovascular disease (cvd) and, experimentally, with measures of atherosclerosis and endothelial dysfunction. periodontal therapy may reduce atherosclerotic changes and improve endothelial function. preliminary findings suggest a role for the genetic lo ...201424736704
combinatorial effects of amoxicillin and metronidazole on selected periodontal bacteria and whole plaque samples.the aim of the present study was to analyze in vitro the combinatorial effects of the antibiotic combination of amoxicillin plus metronidazole on subgingival bacterial isolates.201424727003
an endodontic-periodontal lesion with primary periodontal disease: a case report on its bacterial profile.the microflora in the periodontal pockets can affect the dental pulp and cause endodontic-periodontal lesions or retrograde pulpitis. here we report an endodontic-periodontal lesion together with its bacterial profile. the lesion occurred in the maxillary right first molar of a 40-year-old woman who presented at our hospital complaining of a violent toothache since the previous night. clinically, the tooth was caries-free and an electric pulp test showed it to be vital. the tooth showed signs of ...201424717928
evaluation of the bactericidal activity on oral organisms of the nd-yap laser in vitro.the bactericidal activity of a pulsed fibre-optic nd-yap laser on various strains of oral bacteria: prevotella nigrescens, streptococcus sanguis, actinomyces viscosus (i), fusobacterium nucleatum, actinobacillus actinomycetemcomitans and peptostreptococcus micros (i) was evaluated. a 200-μm optical fibre was placed in eppendorf tubes containing the different bacterial suspensions. at an energy of 170 mj/30 hz, 351 pulses of 150 μs were sufficient to kill the most resistant bacteria (i). the expo ...199824710990
microbial profile of root canals of primary teeth with pulp necrosis and periradicular lesion.the purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion.201424709428
[effect of ultraviolet rays treatment of titanium and titanium alloy on antibacterial property].to evaluate the antibacterial property of ultraviolet rays (uv)-treated titanium and its alloy by observing the attachment and colony-forming units (cfu) of staphylococcus aureus (sa) and fusobacterium nucleatum (fn).201424697888
presence of oral bacterial species in primary endodontic infections of primary teeth.knowledge of the microbial composition of deciduous endodontic infections is limited. this study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions.201324683780
distinct polymicrobial populations in a chronic foot ulcer with implications for diagnostics and anti-infective therapy.polymicrobial infections caused by combinations of different bacteria are being detected with an increasing frequency. the evidence of such complex infections is being revealed through the use of novel molecular and culture-independent methods. considerable progress has been made in the last decade regarding the diagnostic application of such molecular techniques. in particular, 16s rdna-based sequencing and even metagenomic analyses have been successfully used to study the microbial diversity i ...201424679105
oral cavity anaerobic pathogens in biofilm formation on voice prostheses.a polymerase chain reaction (pcr)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. the purpose of the present study was to determine the location of those pathogens inside the biofilms.201524677258
bacteriological analysis of necrotic pulp and fistulae in primary teeth.primary teeth work as guides for the eruption of permanent dentition, contribute for the development of the jaws, chewing process, preparing food for digestion, and nutrient assimilation. treatment of pulp necrosis in primary teeth is complex due to anatomical and physiological characteristics and high number of bacterial species present in endodontic infections. the bacterial presence alone or in association in necrotic pulp and fistula samples from primary teeth of boys and girls was evaluated ...201424676582
massive parallel sequencing provides new perspectives on bacterial brain abscesses.rapid development within the field of massive parallel sequencing (mps) is about to bring this technology within reach for diagnostic microbiology laboratories. we wanted to explore its potential for improving diagnosis and understanding of polymicrobial infections, using bacterial brain abscesses as an example. we conducted a prospective nationwide study on bacterial brain abscesses. fifty-two surgical samples were included over a 2-year period. the samples were categorized as either spontaneou ...201424671797
signaling pathways activation by primary endodontic infectious contents and production of inflammatory mediators.this study investigated the bacterial community involved in primary endodontic diseases, evaluated its ability to activate the macrophage toll-like receptor 4 receptor through p38 mitogen-activated protein kinase (mapk) and nuclear factor kappa b (nf-κb) signaling pathways, and determined the levels of endotoxins and interleukins (interleukin [il]-6 and -10) produced by endodontic content-stimulated macrophages.201424666896
microbiological assessment of the implant-abutment interface in different connections: cross-sectional study after 5 years of functional evaluate the bacterial microflora present inside the implant connection and in the peri-implant sulcus fluid of healthy implants, and to analyze the relationships between these harboring sites for four different implant systems after at least 5 years of functional loading.201524665919
multiplex fluorescence in situ hybridization (m-fish) and confocal laser scanning microscopy (clsm) to analyze multispecies oral biofilms.multiplex fluorescence in situ hybridization (m-fish) constitutes a favorable microbiological method for the analysis of spatial distribution of highly variable phenotypes found in multispecies oral biofilms. the combined use of confocal laser scanning microscopy (clsm) produces high-resolution three-dimensional (3d) images of individual bacteria in their natural environment. here, we describe the application of m-fish on early (streptococcus spp., actinomyces naeslundii) and late colonizers (fu ...201424664826
antimicrobial activities against periodontopathic bacteria of pittosporum tobira and its active compound.the study of medicinal plants for treatment of periodontitis is of great value to establish their efficacy as sources of new antimicrobial drugs. five hundred and fifty eight korean local plant extracts were screened for antibacterial activity against representative periodontopathic bacteria such as porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. among the various medicinal plants, the alcohol extract of pittosporum tobira, which significantly exhibited antibacteria ...201424662076
isolation and identification of porphyromonas spp. and other putative pathogens from cats with periodontal disease.the purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disease. pooled paper point samples from 4 selected subgingival sites were cultured on blood agar and on dentaid-1 medium. suspected pathogens were identif ...201324660305
evaluation of the sealing capability of implants to titanium and zirconia abutments against porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum under different screw torque values.when evaluating long-term implant success, clinicians have always been concerned with the gap at the implant-abutment junction, where bacteria can accumulate and cause marginal bone loss. however, little information regarding bacterial leakage at the implant-abutment junction, or microgap, is available.201424656409
acute appendicitis in children is associated with an abundance of bacteria from the phylum fusobacteria.although luminal obstruction has traditionally been viewed as the underlying cause of appendicitis, recent evidence has suggested that the disease may result directly from invasion by specific pathogens, e.g. fusobacterium nucleatum. the purpose of this study was to survey microbial communities within pediatric appendectomy specimens using a culture-independent approach.201424650474
characterization of aid1, a novel gene involved in fusobacterium nucleatum interspecies interactions.the oral opportunistic pathogen fusobacterium nucleatum is known to interact with a large number of different bacterial species residing in the oral cavity. it adheres to a variety of gram-positive bacteria, including oral streptococci via the arginine-inhibitable adhesin radd. in this study, we describe a novel protein encoded by the predicted open reading frame fn1253 that appears to play a role in interspecies interactions of f. nucleatum, particularly with oral streptococci and related gram- ...201424643713
the antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens.the antibacterial activity of various saturated fatty acids (sfa) and unsaturated fatty acids (usfa) against different oral pathogens which are implicated in the cause of dental caries, stomatitis, gingivitis, and periodontitis was examined. the saturated fatty acids pa, sta and ara, and the unsaturated omega-7 fatty acids pla and omega-9 fatty acids oa showed either none to low antimicrobial activity against all of the 12 oral pathogenic strains used in this study. in contrast, the omega-3 pufa ...201324640241
evaluation of bacterial recovery and viability from three different swab transport systems.this study evaluated three types of swab transport systems for organism recovery. swabs with transport media were further assessed for organism viability over 24  hours over a range of different storage temperatures. test organisms consisted of aerobes, fastidious aerobes and anaerobes. swabs were tested according to the standardised quantitative elution method published by the clinical laboratory standards institute (clsi; m-40a). there were substantial differences in primary organism recovery, ...201424614721
preservation of fusobacterium nucleatum and peptostreptococcus anaerobius dna after loss of cell investigate whether dna from two obligate anaerobes, fusobacterium nucleatum and peptostreptococcus anaerobius, is recoverable after loss of cell viability induced by air exposure.201524611977
fusobacterium nucleatum associates with stages of colorectal neoplasia development, colorectal cancer and disease outcome.commensal bacteria in the colon may play a role in colorectal cancer (crc) development. recent studies from north america showed that fusobacterium nucleatum (fn) infection is over-represented in disease tissue versus matched normal tissue in crc patients. using quantitative real-time polymerase chain reaction (qpcr) of dna extracted from colorectal tissue biopsies and surgical resections of three european cohorts totalling 122 crc patients, we found an over-abundance of fn in cancerous compared ...201424599709
effect of estradiol on planktonic growth, coaggregation, and biofilm formation of the prevotella intermedia group bacteria.alterations in the quantity and quality of biofilms at gingival margin are considered to play a role in the initiation and development of pregnancy-related gingivitis. prevotella intermedia sensu lato is able to consume estradiol, the major sex hormone secreted during pregnancy, in the absence of vitamin k. the aim of the study was to examine the effect of estradiol on the planktonic growth, coaggregation, polysaccharide production, and biofilm formation of the p. intermedia group bacteria, name ...201424594108
coinfection of fusobacterium nucleatum and actinomyces israelii in mastoiditis diagnosed by next-generation dna sequencing.some bacterial infections involve potentially complex mixtures of species that can now be distinguished using next-generation dna sequencing. we present a case of mastoiditis where gram stain, culture, and molecular diagnosis were nondiagnostic or discrepant. next-generation sequencing implicated coinfection of fusobacterium nucleatum and actinomyces israelii, resolving these diagnostic discrepancies.201424574281
diverse toll-like receptors mediate cytokine production by fusobacterium nucleatum and aggregatibacter actinomycetemcomitans in macrophages.toll-like receptors (tlrs) orchestrate a repertoire of immune responses in macrophages against various pathogens. fusobacterium nucleatum and aggregatibacter actinomycetemcomitans are two important periodontal pathogens. in the present study, we investigated tlr signaling regulating cytokine production of macrophages in response to f. nucleatum and a. actinomycetemcomitans. tlr2 and tlr4 are redundant in the production of cytokines (interleukin-6 [il-6] and tumor necrosis factor alpha [tnf-α]) i ...201424566622
lactobacillus salivarius wb21--containing tablets for the treatment of oral malodor: a double-blind, randomized, placebo-controlled crossover trial.this study evaluated the effect of probiotic intervention using lactobacilli on oral malodor.201424556493
biodegradable nanofibrous drug delivery systems: effects of metronidazole and ciprofloxacin on periodontopathogens and commensal oral bacteria.the purposes of this study were to fabricate biodegradable polydioxanone (pds ii®) electrospun periodontal drug delivery systems (hereafter referred to as matrices) containing either metronidazole (met) or ciprofloxacin (cip) and to investigate the effects of antibiotic incorporation on both periodontopathogens and commensal oral bacteria.201424535074
genome sequence of fusobacterium nucleatum strain w1481, a possible new subspecies isolated from a periodontal pocket.fusobacterium nucleatum is a bacterial species commonly detected in dental plaque within the human oral cavity, with some strains associated with periodontal disease, one of the most common clinical bacterial infections in the human body. the exact mechanisms of its pathogenesis are still not completely understood. in this study, we present the genome sequence and annotation of f. nucleatum strain w1481, isolated from a periodontal pocket of a dental patient at the university of bristol, united ...201424526626
inhibition of glutamate racemase by substrate-product analogues.d-glutamate is an essential biosynthetic building block of the peptidoglycans that encapsulate the bacterial cell wall. glutamate racemase catalyzes the reversible formation of d-glutamate from l-glutamate and, hence, the enzyme is a potential therapeutic target. we show that the novel cyclic substrate-product analogue (r,s)-1-hydroxy-1-oxo-4-amino-4-carboxyphosphorinane is a modest, partial noncompetitive inhibitor of glutamate racemase from fusobacterium nucleatum (fngr), a pathogen responsibl ...201424507924
transcriptional responses of treponema denticola to other oral bacterial species.the classic organization by socransky and coworkers categorized the oral bacteria of the subgingival plaque into different complexes. treponema denticola, porphyromonas gingivalis and tannerella forsythia are grouped into the red complex that is highly correlated with periodontal disease. socransky's work closely associates red with orange complex species such as fusobacterium nucleatum and prevotella intermedia but not with members of the other complexes. while the relationship between species ...201424505483
short-chain fatty acids from periodontal pathogens suppress histone deacetylases, ezh2, and suv39h1 to promote kaposi's sarcoma-associated herpesvirus replication.periodontal pathogens such as porphyromonas gingivalis and fusobacterium nucleatum produce five different short-chain fatty acids (scfas) as metabolic by-products. we detect significantly higher levels of scfas in the saliva of patients with severe periodontal disease. the different scfas stimulate lytic gene expression of kaposi's sarcoma-associated herpesvirus (kshv) dose dependently and synergistically. scfas inhibit class-1/2 histone deacetylases (hdacs) and downregulate expression of silent ...201424501407
inhibitory effects of sword bean extract on alveolar bone resorption induced in rats by porphyromonas gingivalis infection.the domesticated legume, canavalia gladiata (commonly called the sword bean), is known to contain canavanine. the fruit is used in chinese and japanese herbal medicine for treating the discharge of pus, but its pharmacological mechanisms are still unclear.201424494651
in vitro growth characteristics and volatile sulfur compound production of solobacterium moorei.solobacterium moorei has recently been implicated as a causative agent of halitosis. in vitro experiments to evaluate the role of s. moorei in halitosis have, however, been complicated by a paucity of information on the ideal conditions for culturing this organism. this work aimed to optimize a liquid culture medium for s. moorei, and to determine the growth-curve of the organism. further, the ability of s. moorei to generate volatile sulfur compounds was investigated and compared quantitatively ...201424487184
prevotella intermedia induces severe bacteremic pneumococcal pneumonia in mice with upregulated platelet-activating factor receptor expression.streptococcus pneumoniae is the leading cause of respiratory infection worldwide. although oral hygiene has been considered a risk factor for developing pneumonia, the relationship between oral bacteria and pneumococcal infection is unknown. in this study, we examined the synergic effects of prevotella intermedia, a major periodontopathic bacterium, on pneumococcal pneumonia. the synergic effects of the supernatant of p. intermedia (pisup) on pneumococcal pneumonia were investigated in mice, and ...201424478074
photodynamic antimicrobial effect of safranine o on an ex vivo periodontal biofilm.the increasing resistance of oral pathogens against antibiotic measures urgently requires new therapeutic strategies. in this context, antimicrobial photodynamic therapy (apdt) may play a crucial part in the future. the aim of the present study was to compare the antibacterial efficiency of apdt using the photosensitizer safranine o with that of chlorhexidine (0.2% chx) on an ex vivo biofilm.201424473989
meningitis due to fusobacterium nucleatum after a kart crash. 201324471173
fusobacterium spondylodiscitis: case report and literature review.fusobacteria are obligate anaerobic bacilli residing in the oral cavity, female genital tract, and intestine. these pathogens are typical components of head, neck, and abdominal abscesses due to contiguous spread from adjacent mucosal surfaces. they are unusual etiologies, however, of bone and joint infections, particularly outside the cranial region. we report an unusual case of hematogenous lumbar spondylodiscitis caused by fusobacterium nucleatum of suspected odontogenic origin.201424462349
preservation of bacterial dna by human dentin.the capacity of dentin and collagen to bind dna and protect against spontaneous and nuclease-induced degradation was evaluated individually and by the incubation of dna with nuclease-producing bacteria in a mixed culture.201424461411
draft genome sequences of fusobacterium nucleatum chdc f145, chdc f174, chdc f206, and chdc f300, isolated from human subgingival plaques in the republic of korea.recently, five strains were isolated from human subgingival plaques and were proposed as a novel subspecies of fusobacterium nucleatum. here, we report the draft genome sequences of the strains, except one for which the draft sequence was already introduced.201424459284
in vitro activity of carvacrol against titanium-adherent oral biofilms and planktonic cultures.the aim of this study was to test the effect of carvacrol against oral pathogens and their preformed biofilms on titanium disc surface.201424458367
co-occurrence of anaerobic bacteria in colorectal carcinomas.numerous cancers have been linked to microorganisms. given that colorectal cancer is a leading cause of cancer deaths and the colon is continuously exposed to a high diversity of microbes, the relationship between gut mucosal microbiome and colorectal cancer needs to be explored. metagenomic studies have shown an association between fusobacterium species and colorectal carcinoma. here, we have extended these studies with deeper sequencing of a much larger number (n = 130) of colorectal carcinoma ...201324450771
radiography-based score indicative for the pathogenicity of bacteria in odontogenic develop a new radiography-based score to assess the potential of bacteria to cause odontogenic infections derived from the occurrence of bacteria at small or large radiographical lesions.201424444257
effects of tongue cleaning on bacterial flora in tongue coating and dental plaque: a crossover study.the effects of tongue cleaning on reconstruction of bacterial flora in dental plaque and tongue coating itself are obscure. we assessed changes in the amounts of total bacteria as well as fusobacterium nucleatum in tongue coating and dental plaque specimens obtained with and without tongue cleaning.201424423407
detection of fusobacterium nucleatum and fada adhesin gene in patients with orthodontic gingivitis and non-orthodontic periodontal inflammation.fusobacterium nucleatum is one of the most abundant gram-negative bacilli colonizing the subgingival plaque and closely associated with periodontal disease. however it is unclear whether f. nucleatum is involved in gingival inflammation under orthodontic appliance. a novel adhesin, fada, which is unique to oral fusobacteria, is required for f. nucleatum binding and invasion to epithelial cells and thus may play an important role in colonization of fusobacterium in the host. in this study, we eva ...201424416378
antibacterial and antigelatinolytic effects of satureja hortensis l. essential oil on epithelial cells exposed to fusobacterium nucleatum.the present report examined the effects of essential oils (eos) from satureja hortensis l. and salvia fruticosa m. on the viability and outer membrane permeability of the periodontopathogen fusobacterium nucleatum, a key bacteria in oral biofilms, as well as the inhibition of matrix metalloproteinase (mmp-2 and mmp-9) activities in epithelial cells exposed to such bacteria. membrane permeability was tested by measuring the n-phenyl-1-naphthylamine uptake and bacterial viability by using the comm ...201524404975
gene-diet interactions on colorectal cancer risk.there has been increasing interest lately in understanding how natural dietary antioxidants affect chemoprevention, and recently, there has been a merging of information about antioxidants, endogenous and exogenous reactive oxygen and nitrogen species (rons), and inflammation. rons normally serve the cells as second messengers to regulate many of the intracellular signaling cascades that govern multiple cellular activities. however, when the amount of rons exceeds the cell's ability to metaboliz ...201224392268
periodontal bacteria in the genital tract: are they related to adverse pregnancy outcome?one of the most important factors implicated in preterm birth (ptb) is acute genitourinary tract infection. the bacteria causing chronic periodontal inflammation include gram-negative rods and anaerobes similar to those found in women with bacterial vaginosis. the aim of this prospective study is to investigate the relationship between oral and vaginal microflora and preterm low birth weight. real-time polymerase chain reaction was used to detect both the presence and level of six periodontitis- ...201724355228
the effects of a mineral trioxide aggregate-based sealer on the production of reactive oxygen species, nitrogen species and cytokines by two macrophage test the effects of a mineral trioxide aggregate-based sealer (mta fillapex(®)) and mta (mta-ângelus(®)) on viability and on the production of cytokines, reactive oxygen species (ros) and nitrogen species (no) by m1 and m2 inflammatory macrophages.201424354338
phototargeting human periodontal pathogens in vivo.the effects of blue light at 455 nm were investigated on the bacterial composition of human dental plaque in vivo. eleven subjects who refrained from brushing for 3 days before and during phototherapy participated in the study. light with a power density of 70 mw/cm(2) was applied to the buccal surfaces of premolar and molar teeth on one side of the mouth twice daily for 2 min over a period of 4 days. dental plaque was harvested at baseline and again at the end of 4 days from eight posterior tee ...201524346334
impact of early colonizers on in vitro subgingival biofilm formation.the aim of this study was to investigate the impact of early colonizing species on the structure and the composition of the bacterial community developing in a subgingival 10-species biofilm model system. the model included streptococcus oralis, streptococcus anginosus, actinomycesoris, fusobacterium nucleatum subsp. nucleatum, veillonella dispar, campylobacter rectus, prevotella intermedia, porphyromonas gingivalis, tannerella forsythia, and treponema denticola. based on literature, we consider ...201324340084
draft genome sequence of fusobacterium nucleatum subsp. animalis chdc f324, isolated from a human subgingival plaque in the republic of korea.five subspecies of fusobacterium nucleatum have been classified: animalis, nucleatum, polymorphum, vincentii, and fusiforme. f. nucleatum subsp. animalis chdc f324 (kcom 1325) was isolated from a human subgingival plaque in the republic of korea. here, we report the draft genome sequence of the strain.201324336380
draft genome sequence of fusobacterium nucleatum subsp. nucleatum chdc f316, isolated from a human peri-implantitis lesion in the republic of korea.fusobacterium nucleatum is a gram-negative anaerobe and is one of the causative agents of periodontal diseases, including peri-implantitis. fusobacterium nucleatum subsp. nucleatum chdc f316 (kcom 1322) was isolated from a human peri-implantitis lesion. here, we report the draft genome sequence of this strain.201324336379
draft genome sequence of fusobacterium nucleatum subsp. vincentii chdc f8, isolated from a human subgingival plaque in the republic of korea.fusobacterium nucleatum is a gram-negative, nonmotile, obligately anaerobic rod bacterium which might play an important role in the initiation and progression of periodontal diseases. f. nucleatum subsp. vincentii chdc f8 (kcom 1231) was isolated from a human gingivitis lesion. here, we report the draft genome sequence of the strain.201324336378
dental caries and microbiota in children with black stain and non-discoloured dental plaque.we aimed to assess caries experience and microbiota in systemically healthy children with black stain (bs) and non-discoloured plaque.201424335143
fusobacterium nucleatum activates the immune response through retinoic acid-inducible gene i.retinoic acid-inducible gene i (rig-i) is a cytosolic pattern recognition receptor involved in the sensing of rna viruses and the initiation of antiviral responses. fusobacterium nucleatum, a gram-negative anaerobic bacterium associated with periodontal disease, is capable of invading cells. we hypothesized that f. nucleatum's ability to invade cells allows the microorganism to activate the immune response through rig-i. bacterial invasion was found to be necessary for f. nucleatum-induced nucle ...201424334410
analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences.201324278920
association of plasma 25-hydroxyvitamin d concentrations and pathogenic oral bacteria in postmenopausal females.previous findings of an association between 25-hydroxyvitamin d [25(oh)d] concentrations and periodontal disease may be partially explained by the antimicrobial properties of vitamin d. to the best of the authors' knowledge, no study has investigated the association between 25(oh)d and pathogenic oral bacteria, a putative cause of periodontal disease.201324261910
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