Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bacteriological analysis of necrotic pulp and fistulae in primary teeth. | primary teeth work as guides for the eruption of permanent dentition, contribute for the development of the jaws, chewing process, preparing food for digestion, and nutrient assimilation. treatment of pulp necrosis in primary teeth is complex due to anatomical and physiological characteristics and high number of bacterial species present in endodontic infections. the bacterial presence alone or in association in necrotic pulp and fistula samples from primary teeth of boys and girls was evaluated ... | 2014 | 24676582 |
| oral cavity anaerobic pathogens in biofilm formation on voice prostheses. | a polymerase chain reaction (pcr)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. the purpose of the present study was to determine the location of those pathogens inside the biofilms. | 2015 | 24677258 |
| distinct polymicrobial populations in a chronic foot ulcer with implications for diagnostics and anti-infective therapy. | polymicrobial infections caused by combinations of different bacteria are being detected with an increasing frequency. the evidence of such complex infections is being revealed through the use of novel molecular and culture-independent methods. considerable progress has been made in the last decade regarding the diagnostic application of such molecular techniques. in particular, 16s rdna-based sequencing and even metagenomic analyses have been successfully used to study the microbial diversity i ... | 2014 | 24679105 |
| presence of oral bacterial species in primary endodontic infections of primary teeth. | knowledge of the microbial composition of deciduous endodontic infections is limited. this study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. | 2013 | 24683780 |
| [effect of ultraviolet rays treatment of titanium and titanium alloy on antibacterial property]. | to evaluate the antibacterial property of ultraviolet rays (uv)-treated titanium and its alloy by observing the attachment and colony-forming units (cfu) of staphylococcus aureus (sa) and fusobacterium nucleatum (fn). | 2014 | 24697888 |
| microbial profile of root canals of primary teeth with pulp necrosis and periradicular lesion. | the purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion. | 2014 | 24709428 |
| evaluation of the bactericidal activity on oral organisms of the nd-yap laser in vitro. | the bactericidal activity of a pulsed fibre-optic nd-yap laser on various strains of oral bacteria: prevotella nigrescens, streptococcus sanguis, actinomyces viscosus (i), fusobacterium nucleatum, actinobacillus actinomycetemcomitans and peptostreptococcus micros (i) was evaluated. a 200-μm optical fibre was placed in eppendorf tubes containing the different bacterial suspensions. at an energy of 170 mj/30 hz, 351 pulses of 150 μs were sufficient to kill the most resistant bacteria (i). the expo ... | 1998 | 24710990 |
| an endodontic-periodontal lesion with primary periodontal disease: a case report on its bacterial profile. | the microflora in the periodontal pockets can affect the dental pulp and cause endodontic-periodontal lesions or retrograde pulpitis. here we report an endodontic-periodontal lesion together with its bacterial profile. the lesion occurred in the maxillary right first molar of a 40-year-old woman who presented at our hospital complaining of a violent toothache since the previous night. clinically, the tooth was caries-free and an electric pulp test showed it to be vital. the tooth showed signs of ... | 2014 | 24717928 |
| combinatorial effects of amoxicillin and metronidazole on selected periodontal bacteria and whole plaque samples. | the aim of the present study was to analyze in vitro the combinatorial effects of the antibiotic combination of amoxicillin plus metronidazole on subgingival bacterial isolates. | 2014 | 24727003 |
| periodontal disease, atherosclerosis, adverse pregnancy outcomes, and head-and-neck cancer. | interrelationships between periodontal infection and systemic conditions such as cardiovascular disease, adverse pregnancy outcomes, and head-and-neck cancer have become increasingly appreciated in recent years. periodontitis is associated with cardiovascular disease (cvd) and, experimentally, with measures of atherosclerosis and endothelial dysfunction. periodontal therapy may reduce atherosclerotic changes and improve endothelial function. preliminary findings suggest a role for the genetic lo ... | 2014 | 24736704 |
| antibacterial action of chlorhexidine/thymol containing varnishes in vitro and in vivo. | the antibacterial activity of two different formulations of a chlorhexidine/thymol varnish should be elucidated in vitro and in vivo. | 2014 | 24738759 |
| new bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. | the aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings. | 2014 | 24767562 |
| salivary pellicle composition and multispecies biofilm developed on titanium nitrided by cold plasma. | the aim of this study was to evaluate the composition of the salivary pellicle (sp) and multispecies biofilm developed on titanium nitrided by cold plasma. | 2014 | 24769315 |
| in vitro evaluation of a multispecies oral biofilm on different implant surfaces. | biofilm accumulation on implant surfaces is one of the most important factors for early and late implant failure. because of the related clinical implications, the aim of this in vitro study was to compare the bacterial cell attachment of a four-species oral biofilm on titanium discs of purity grade 2 and 4, with machined surfaces and etched-thermochemically modified with avantblast®. the in vitro biofilm model was composed of early (actinomyces naeslundii, streptococcus gordonii), secondary (ve ... | 2014 | 24770899 |
| red wine and oenological extracts display antimicrobial effects in an oral bacteria biofilm model. | the antimicrobial effects of red wine and its inherent components on oral microbiota were studied by using a 5-species biofilm model of the supragingival plaque that includes actinomyces oris, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar. microbiological analysis (cfu counting and confocal laser scanning microscopy) of the biofilms after the application of red wine, dealcoholized red wine, and red wine extract solutions spiked or not with grape seed ... | 2014 | 24773294 |
| a periodontitis-associated multispecies model of an oral biofilm. | while single-species biofilms have been studied extensively, we know notably little regarding multispecies biofilms and their interactions. the purpose of this study was to develop and evaluate an in vitro multispecies dental biofilm model that aimed to mimic the environment of chronic periodontitis. | 2014 | 24778902 |
| microbial mucosal colonic shifts associated with the development of colorectal cancer reveal the presence of different bacterial and archaeal biomarkers. | epidemiological studies demonstrate a link between gastrointestinal cancers and environmental factors such as diet. it has been suggested that environmental cancer risk is determined by the interaction between diet and microbes. thus, the purpose of this study was to examine the hypothesis that microbiota composition during colorectal cancer (crc) progression might differ depending on the stage of the disease. | 2015 | 24811328 |
| relationship between expression of human gingival beta-defensins and levels of periodontopathogens in subgingival plaque. | human beta-defensins (hbds) are a group of antimicrobial peptides important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. the aim of this study was to explore relationships among hbds, total subgingival bacteria and periodontopathogens in healthy subjects and in patients with chronic periodontitis. | 2015 | 24814979 |
| vertebral osteomyelitis caused by fusobacterium nucleatum: a case report. | a 58-year-old man with a history of chronic alcohol abuse presented with vertebral osteomyelitis caused by fusobacterium nucleatum. this case demonstrated the foul smelling abscess characteristic of anaerobic infection. deep anaerobic infections are well known to be resistant to closed treatment, and the computerized tomographic scan confirmed the diagnosis of a paravertebral abscess in this case. an increased awareness among clinicians that anaerobic bacilli can cause oteomyelitis, together wit ... | 1979 | 24823152 |
| subgingival bacteria in ghanaian adolescents with or without progression of attachment loss. | this study describes subgingival bacterial profiles associated with clinical periodontal status in ghanaian adolescents with or without progression of attachment loss. | 2014 | 24834145 |
| evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time pcr. | the aim of this study was to investigate the mandibular third molar pericoronitis flora by using real-time polymerase chain reaction (pcr). | 2014 | 24835305 |
| intestinal dysbiosis: novel mechanisms by which gut microbes trigger and prevent disease. | new research has identified specific intestinal colonizing microbes that can have significant influence on health and disease. evidence is reviewed supporting an association between fusobacterium nucleatum and colon cancer and for a protective role of faecalibacterium prausnitzii in inflammatory bowel disease, of escherichia coli nissle 1917 in acute intestinal inflammation, of bifidobacterium infantis in neonatal necrotizing enterocolitis, and of akkermansia muciniphila in obesity and diabetes. ... | 2014 | 24857830 |
| clinical and microbiological characterization of localized aggressive periodontitis: a cohort study. | localized aggressive periodontitis (lagp) is an infectious periodontal disease which generally affects young people. recent data suggest the involvement of different bacterial species in different populations. the causative bacterial species in israel has never been identified despite a high prevalence of lagp in this population. the objectives of this study were to characterize the bacterial microbiota of periodontal pockets within an israeli lagp population who were also clinically assessed. | 2014 | 24861390 |
| internal bacterial colonization of implants: association with peri-implant bone loss. | the aim of the present longitudinal study was to investigate bacterial colonization of the internal implant cavity and to evaluate a possible association with peri-implant bone loss. | 2015 | 24861845 |
| microbial evaluation of traumatized teeth treated with triple antibiotic paste or calcium hydroxide with 2% chlorhexidine gel in pulp revascularization. | revascularization outcome depends on microbial elimination because apical repair will not happen in the presence of infected tissues. this study evaluated the microbial composition of traumatized immature teeth and assessed their reduction during different stages of the revascularization procedures performed with 2 intracanal medicaments. | 2014 | 24862703 |
| the kampo medicine rokumigan possesses antibiofilm, anti-inflammatory, and wound healing properties. | periodontal diseases, which are inflammatory diseases of bacterial origin affecting the tooth-supporting tissues, are characterized by inflammation and destruction of gingival connective tissue and alveolar bone, and may lead to tooth loss. the aim of the study was to investigate rokumigan, a kampo japanese traditional medicine made of six different plants, for its capacity to prevent biofilm formation by fusobacterium nucleatum, to inhibit interleukin-6 (il-6) and interleukin-8 (il-8) secretion ... | 2014 | 24877093 |
| the antibiofilm activity of lingonberry flavonoids against oral pathogens is a case connected to residual complexity. | the antimicrobial activity of lingonberry (vaccinium vitis-idaea l.) was evaluated against two oral pathogens, streptococcus mutans and fusobacterium nucleatum. long-bed gel permeation chromatography (gpc; sephadex lh-20) yielded purified flavonoids, with the most efficient minimum inhibitory concentrations (mics) against planktonic cells in the anthocyanin and procyanidin primary fractions against f. nucleatum (63-125 μg/ml) and in the procyanidin rich fraction against s. mutans (16-31 μg/ml). ... | 2014 | 24879903 |
| ubiquitous sialometabolism present among oral fusobacteria. | fusobacterium nucleatum is a ubiquitous member of the human oral flora and is associated with the development of periodontitis and a variety of other types of polymicrobial infections of the mucosa. in the oral cavity, this species is one of the few that is prevalent in both healthy and diseased subgingival plaque. using microarray analysis, we examined the transcriptional response of f. nucleatum subspecies nucleatum to whole blood in order to identify some of the genetic responses that might o ... | 2014 | 24896093 |
| the interaction of implant luting cements and oral bacteria linked to peri-implant disease: an in vitro analysis of planktonic and biofilm growth--a preliminary study. | there is little consensus on the most appropriate cement to use when restoring a cement-retained, implant-supported restoration. one consideration should be the interaction of pathogenic oral bacteria with restorative cements. | 2015 | 24909337 |
| rapid brain death caused by a cerebellar abscess with fusobacterium nucleatum in a young man with drug abuse: a case report. | fusobacterium nucleatum is a strict anaerobic microorganism that causes disease entities such as periodontal and soft tissue abscesses, pulmonary and intraabdominal infections and very rarely intracerebral infections. | 2014 | 24915846 |
| effects of stress hormones on the production of volatile sulfur compounds by periodontopathogenic bacteria. | little is known about the effects of stress hormones on the etiologic agents of halitosis. thus, the aim of this study was to evaluate in vitro the effects of adrenaline (adr), noradrenaline (na) and cortisol (cort) on bacteria that produce volatile sulfur compounds (vsc), the major gases responsible for bad breath. cultures of fusobacterium nucleatum (fn), porphyromonas endodontalis (pe), prevotella intermedia (pi) and porphyromonas gingivalis (pg) were exposed to 50 µm adr, na and cort or equi ... | 2014 | 24918364 |
| in vitro efficacy of cefovecin against anaerobic bacteria isolated from subgingival plaque of dogs and cats with periodontal disease. | periodontal disease is a common disease of dogs and cats often requiring antimicrobial treatment as an adjunct to mechanical debridement. however, correct compliance with oral antimicrobial therapy in companion animals is often difficult. cefovecin is a recently introduced veterinary cephalosporin that has demonstrated prolonged concentrations in extracellular fluid, allowing for dosing intervals of up to 14 days. subgingival samples were collected from the oral cavity of 29 dogs and eight cats ... | 2014 | 24930431 |
| gastrointestinal variant of lemierre syndrome: fusobacterium nucleatum bacteremia-associated hepatic vein thrombosis: a case report and literature review. | fusobacterium nucleatum is a gram-negative bacillius commonly found in oropharynx and is traditionally associated with lemierre syndrome, which is characterized by history of recent oropharyngeal infection, internal jugular vein thrombosis, and isolation of anaerobic pathogens, mainly fuosobacterium necrophorum. however, recent evidence indicated that f. nucleatum is also a normal resident of human gut. less than a dozen of case reports had linked f. nucleatum to gastrointestinal variant of lemi ... | 2017 | 24942004 |
| the influence of orthodontic fixed appliances on the oral microbiota: a systematic review. | to investigate whether there is scientific evidence to support the hypothesis that the presence of orthodontic fixed appliances influences the oral microbiota. | 2014 | 24945514 |
| colonisation of gingival epithelia by subgingival biofilms in vitro: role of "red complex" bacteria. | biofilm formation on tooth surface results in colonisation and invasion of the juxtaposed gingival tissue, eliciting strong inflammatory responses that lead to periodontal disease. this in vitro study investigated the colonisation of human gingival multi-layered epithelium by multi-species subgingival biofilms, and evaluated the relative effects of the "red complex" species (porphyromonas gingivalis, tannerella forsythia and treponema denticola). | 2014 | 24949828 |
| identification of occult fusobacterium nucleatum central nervous system infection by use of pcr-electrospray ionization mass spectrometry. | anaerobic bacteria are often difficult to detect, especially after the initiation of antibiotics. we describe the application of pcr-electrospray ionization mass spectrometry (pcr/esi-ms) using a sample of cerebrospinal fluid to identify an anaerobic gram-negative bacillus, fusobacterium nucleatum, in a patient with "culture-negative" meningitis and cerebral abscesses. | 2014 | 24966358 |
| fusobacterium and enterobacteriaceae: important players for crc? | the gut microbiota plays an essential role in regulating intestinal homeostasis through its capacity to modulate various biological activities ranging from barrier, immunity and metabolic function. not surprisingly, microbial dysbiosis is associated with numerous intestinal disorders including inflammatory bowel diseases (ibd) and colorectal cancer (crc). in this piece, we will review recent evidence that gut microbial dysbiosis can influence intestinal disease, including colitis and crc. we wil ... | 2014 | 24972311 |
| biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial-stimulated pdl cells. | the present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (pdl) cells in the presence of bacterial challenge. cells were seeded on bioflex culture plates and exposed to fusobacterium nucleatum atcc 25586 and/or cyclic tensile strain (cts) of low (ctsl) and high (ctsh) magnitudes for 1 and 3 days. synthesis of cyclooxygenase-2 (cox2) and prostaglandin e2 (pge2) was evaluated by elisa. gene ... | 2014 | 24976684 |
| bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site. | sialic acids are a family of related nine-carbon sugar acids that play important roles in both eukaryotes and prokaryotes. these sialic acids are incorporated/decorated onto lipooligosaccharides as terminal sugars in multiple bacteria to evade the host immune system. many pathogenic bacteria scavenge sialic acids from their host and use them for molecular mimicry. the first step of this process is the transport of sialic acid to the cytoplasm, which often takes place using a tripartite atp-indep ... | 2014 | 25004958 |
| susceptibility of methacrylate-based root canal filling to degradation by bacteria found in endodontic infections. | to present a case of endodontic failure obturated with a methacrylate-based root filling material, resilon/realseal (rs). to determine if rs is susceptible to biodegradation by endodontically relevant microbes by a method known to show rs degradation. | 2014 | 25019109 |
| pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils. | endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. to investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections (endodontic pathogens [ep]), i.e., fusobacterium nucleatum, streptococcus intermedius, parvimonas micra, and prevotella intermedia, was inoculated into subcutaneousl ... | 2014 | 25024367 |
| high-level antimicrobial efficacy of representative mediterranean natural plant extracts against oral microorganisms. | nature is an unexplored reservoir of novel phytopharmaceuticals. since biofilm-related oral diseases often correlate with antibiotic resistance, plant-derived antimicrobial agents could enhance existing treatment options. therefore, the rationale of the present report was to examine the antimicrobial impact of mediterranean natural extracts on oral microorganisms. five different extracts from olea europaea, mastic gum, and inula viscosa were tested against ten bacteria and one candida albicans s ... | 2014 | 25054150 |
| active invasion of porphyromonas gingivalis and infection-induced complement activation in apoe-/- mice brains. | periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of alzheimer's disease. apoe-/- mice were orally infected (n = 12) with porphyromonas gingivalis, treponema denticola, tannerella forsythia, and fusobacterium nucleatum as mono- and polymicrobial infections. apoe-/- mice were sacrificed following 12 and 24 weeks of chronic infection. bacterial gen ... | 2015 | 25061055 |
| microbial community in persistent apical periodontitis: a 16s rrna gene clone library analysis. | to characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. | 2015 | 25088120 |
| a comparative antimicrobial analysis of various root canal irrigating solutions on endodontic pathogens: an in vitro study. | evolution in understanding the poly-microbial environment of both endodontic infections and that of failed root canal treatments has been debatable over the years. the present study was designed to compare and analyze the effect of various root canal irrigation solutions on certain endodontic pathogens in vitro. | 2014 | 25095835 |
| reducing the bioactivity of tannerella forsythia lipopolysaccharide by porphyromonas gingivalis. | tannerella forsythia is considered a pathogen of periodontitis and forms a biofilm with multi-species bacteria in oral cavity. lipopolysaccharide is a powerful immunostimulator and induces inflammation and shock. the purpose of this study was to investigate the characteristics of t. forsythia lps in its co-cultivation with fusobacterium nucleatum or porphyromonas gingivalis. t. forsythia was co-cultured in the presence and absence of f. nucleatum and p. gingivalis and then t. forsythia lps was e ... | 2014 | 25098564 |
| effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults. | oral bacteria live in symbiosis with the host. therefore, when mouthwashes are indicated, selective inhibition of taxa contributing to disease is preferred instead of broad-spectrum antimicrobials. the potential selectivity of an oxygenating mouthwash, ardox-x® (ax), has not been assessed. the aim of this study was to determine the antimicrobial potential of ax and the effects of a twice-daily oral rinse on dental plaque composition. | 2014 | 25101249 |
| fusobacterium detected in colonic biopsy and clinicopathological features of ulcerative colitis in japan. | fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. here, we performed prevalence screening of fusobacterium in ulcerative colitis (uc) in japanese patients. | 2015 | 25102986 |
| axenic culture of a candidate division tm7 bacterium from the human oral cavity and biofilm interactions with other oral bacteria. | the diversity of bacterial species in the human oral cavity is well recognized, but a high proportion of them are presently uncultivable. candidate division tm7 bacteria are almost always detected in metagenomic studies but have not yet been cultivated. in this paper, we identified candidate division tm7 bacterial phylotypes in mature plaque samples from around orthodontic bonds in subjects undergoing orthodontic treatment. successive rounds of enrichment in laboratory media led to the isolation ... | 2014 | 25107981 |
| an in vitro biofilm model associated to dental implants: structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces. | the impact of implant surfaces in dental biofilm development is presently unknown. the aim of this investigation was to assess in vitro the development of a complex biofilm model on titanium and zirconium implant surfaces, and to compare it with the same biofilm formed on hydroxyapatite surface. | 2014 | 25110288 |
| individual growth detection of bacterial species in an in vitro oral polymicrobial biofilm model. | most in vitro studies on the antibacterial effects of antiseptics have used planktonic bacteria in monocultures. however, this study design does not reflect the in vivo situation in oral cavities harboring different bacterial species that live in symbiotic relationships in biofilms. the aim of this study was to establish a simple in vitro polymicrobial model consisting of only three bacterial strains of different phases of oral biofilm formation to simulate in vivo oral conditions. therefore, we ... | 2014 | 25119373 |
| increased proliferation and decreased membrane permeability as defense mechanisms of fusobacterium nucleatum against human neutrophilic peptide-1. | human neutrophilic peptides (hnps) constitute a class of host defense molecules, which contribute to the non-oxidative killing of bacteria and other microorganisms. since the adaptability is crucial to bacterial survival in changing environments, it is of interest to know how fusobacterium nucleatum, the major bridge organism connecting early and late colonizers in dental biofilms, defends itself against hnps. this study aimed to examine the planktonic growth, membrane permeability, and biofilm ... | 2014 | 25132418 |
| microbial signature profiles of periodontally healthy and diseased patients. | to determine microbial profiles that discriminate periodontal health from different forms of periodontal diseases. | 2014 | 25139407 |
| proteomics of fusobacterium nucleatum within a model developing oral microbial community. | fusobacterium nucleatum is a common oral organism that can provide adhesive and metabolic support to developing periodontal bacterial communities. it is within the context of these communities that disease occurs. we have previously reported whole cell proteomics analyses of porphyromonas gingivalis and streptococcus gordonii in early-stage communities with each other and with f. nucleatum, modeled using 18 h pellets. here, we report the adaptation of f. nucleatum to the same experimental condit ... | 2014 | 25155235 |
| nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms. | chlorhexidine (chx) is a widely used antimicrobial agent in dentistry. herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure chx (nano-chx), and its mechanical profile and antimicrobial properties against oral biofilms. | 2014 | 25170958 |
| the presence of cariogenic and periodontal pathogens in the oral cavity of one-year-old infants delivered pre-term with very low birthweights: a case control study. | recently, the dental literature has focused mainly on the microbial colonization of healthy full-term infants and their mothers or caretakers. however, oral microbial acquisition by premature infants has not been adequately investigated, and the correlation between pre-term birth and the presence of cariogenic and periodontal pathogens has not been determined. the aim of this study was to identify the presence and quantities of representative cariogenic and periodontal pathogens in the oral cavi ... | 2014 | 25178742 |
| global analysis of saliva as a source of bacterial genes for insights into human population structure and migration studies. | the genetic diversity of the human microbiome holds great potential for shedding light on the history of our ancestors. helicobacter pylori is the most prominent example as its analysis allowed a fine-scale resolution of past migration patterns including some that could not be distinguished using human genetic markers. however studies of h. pylori require stomach biopsies, which severely limits the number of samples that can be analysed. by focussing on the house-keeping gene gdh (coding for the ... | 2014 | 25183372 |
| the effect of sodium hypochlorite on enterococcus faecalis when grown on dentine as a single- and multi-species biofilm. | enterococcus faecalis is often involved in the aetiology of apical periodontitis after endodontic treatment. this project aimed to establish, on dentine in vitro, a multi-species biofilm containing e. faecalis, and to determine if the organism had an increased resistance to sodium hypochlorite compared with an axenic biofilm. biofilms were established on dentine discs in flow cells with either e. faecalis alone (axenic) or together with fusobacterium nucleatum and streptococcus sanguinis. follow ... | 2014 | 25195495 |
| bimix antimicrobial scaffolds for regenerative endodontics. | eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. the aim of this study was to synthesize and determine in vitro both the antimicrobial effectiveness and cytocompatibility of bimix antibiotic-containing polydioxanone-based polymer scaffolds. | 2014 | 25201643 |
| real-time pcr quantification of six periodontal pathogens in saliva samples from healthy young adults. | the use of saliva as a diagnostic fluid for the evaluation of periodontal health has gained attention recently. most published real-time pcr assays focused on quantification of bacteria in subgingival plaque, not in saliva. the aims of this study were to develop a real-time pcr assay for quantification of six periodontal pathogens in saliva and to establish a relationship between the amount of dna (fg) and colony-forming unit (cfu). | 2015 | 25217278 |
| association between polycystic ovary syndrome, oral microbiota and systemic antibody responses. | polycystic ovary syndrome (pcos) is a hormonal disorder of women that not only is the leading cause of infertility but also shows a reciprocal link with oral health. this study aimed to investigate the hypothesis that the levels of putative periodontal pathogens in saliva and their antibody response in serum are elevated in pcos, compared to systemic health. a total of 125 women were included in four groups; 45 women with pcos and healthy periodontium, 35 women with pcos and gingivitis, 25 syste ... | 2014 | 25232962 |
| effect of bdellovibrio bacteriovorus hd100 on multispecies oral communities. | the predation of bdellovibrio bacteriovorus on different periodontal pathogens has already been described. however, it is necessary to consider the polymicrobial nature of periodontal disease. the current study explores the predation of bdellovibrio on oral pathogens organized in multispecies communities. the effect of the predator was evaluated on in vitro six species communities with microbial culturing. additionally, the effect on ex vivo subgingival plaque and saliva samples from periodontit ... | 2015 | 25252124 |
| [effects of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions]. | to investigate the effect of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions. | 2014 | 25263382 |
| hydrogen sulfide production from subgingival plaque samples. | periodontitis is a polymicrobial anaerobe infection. little is known about the dysbiotic microbiota and the role of bacterial metabolites in the disease process. it is suggested that the production of certain waste products in the proteolytic metabolism may work as markers for disease severity. hydrogen sulfide (h2s) is a gas produced by degradation of proteins in the subgingival pocket. it is highly toxic and believed to have pro-inflammatory properties. we aimed to study h2s production from su ... | 2015 | 25280920 |
| subgingival microbiome in smokers and non-smokers in korean chronic periodontitis patients. | smoking is a major environmental factor associated with periodontal diseases. however, we still have a very limited understanding of the relationship between smoking and subgingival microflora in the global population. here, we investigated the composition of subgingival bacterial communities from the pooled plaque samples of smokers and non-smokers, 134 samples in each group, in korean patients with moderate chronic periodontitis using 16s rrna gene-based pyrosequencing. a total of 17,927 reads ... | 2015 | 25283067 |
| influence of highly-active antiretroviral therapy on the subgingival biofilm in hiv-infected patients. | highly-active antiretroviral therapy (haart) has been associated with alterations in subgingival biofilm and periodontal disease. the purpose of the present study was to investigate the association between different haart regimens and the prevalence of periodontal pathogens in hiv-infected patients with chronic periodontitis. | 2016 | 25283543 |
| relationship of clinical and microbiological variables in patients with type 1 diabetes mellitus and periodontitis. | the aim of the study was to analyze how metabolic control of type 1 diabetes is related to clinical and microbiological periodontal parameters. | 2014 | 25294115 |
| effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis. | short chain fatty acids (scfas) play important roles in periodontal diseases. however, the concentrations of scfas in gingival crevicular fluid of patients with aggressive periodontitis are not known. the aim of this intervention study was to investigate the influences of non-surgical periodontal therapy on levels of scfas in the gingival crevicular fluid of patients with generalized aggressive periodontitis (g-agp), and analyze the concentrations of scfas in sites with or without the detected p ... | 2014 | 25340203 |
| antimicrobial activity of platelet-rich plasma and other plasma preparations against periodontal pathogens. | in addition to releasing a pool of growth factors during activation, platelets have many features that indicate their role in the anti-infective host defense. the antimicrobial activities of platelet-rich plasma (prp) and related plasma preparations against periodontal disease-associated bacteria were evaluated. | 2015 | 25345340 |
| identification and characterization of fusolisin, the fusobacterium nucleatum autotransporter serine protease. | fusobacterium nucleatum is an oral anaerobe associated with periodontal disease, adverse pregnancy outcomes and colorectal carcinoma. a serine endopeptidase of 61-65 kda capable of damaging host tissue and of inactivating immune effectors was detected previously in f. nucleatum. here we describe the identification of this serine protease, named fusolisin, in three oral f. nucleatum sub-species. gel zymogram revealed fusobacterial proteolytic activity with molecular masses ranging from 55-101 kda ... | 2014 | 25357190 |
| interactions of anaerobic bacteria with dental stem cells: an in vitro study. | in patients with periodontitis, it is highly likely that local (progenitor) cells encounter pathogenic bacteria. the purpose of this in vitro study was to elucidate how human dental follicle stem cells (hdfsc) react towards a direct challenge with anaerobic periodontal pathogens under their natural oxygen-free atmosphere. hdfsc were compared to human bone marrow mesenchymal stem cells (hbmsc) and differentiated primary human gingival fibroblasts (hgif), as well as permanent gingival carcinoma ce ... | 2014 | 25369260 |
| essential oils and isolated compounds from lippia alba leaves and flowers: antimicrobial activity and osteoclast apoptosis. | in the present study, essential oils extracted from the leaves and flowers of lippia alba (mill.) n.e.br. (l. alba) were analyzed for their antimicrobial activity and their effects on osteoclasts. the periodontal pathogens, aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans; atcc 43717), fusobacterium nucleatum (f. nucleatum; atcc 25586) and porphyromonas gingivalis (p. gingivalis); atcc 33277) were used in antimicrobial activity assays for determining the minimum inhibitory concent ... | 2015 | 25384405 |
| prevalence of six periodontal pathogens in subgingival samples of italian patients with chronic periodontitis. | the aim of this study was to investigate the subgingival prevalence of six periodontal pathogens in 352 italian patients with chronic periodontitis. possible correlations with clinical parameters, age, gender and smoking status were also investigated. at first visit a pooled subgingival plaque sample was obtained for each subject by using the paper-point method. the samples were processed and analysed according to a commercially available quantitative real time polymerase chain reaction assay (m ... | 2014 | 25387289 |
| periodontal and peri-implant microbiota in patients with healthy and inflamed periodontal and peri-implant tissues. | to compare the prevalence and levels of six bacterial pathogens within the subgingival/submucosal microbiota at teeth versus implants with various clinical conditions. | 2016 | 25399962 |
| diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three gram-negative bacteria. | nad-dependent d-lactate dehydrogenases (d-ldhs) reduce pyruvate into d-lactate with oxidation of nadh into nad(+). although non-allosteric d-ldhs from lactobacilli have been extensively studied, the catalytic properties of allosteric d-ldhs from gram-negative bacteria except for escherichia coli remain unknown. we characterized the catalytic properties of d-ldhs from three gram-negative bacteria, fusobacterium nucleatum (fnldh), pseudomonas aeruginosa (paldh), and e. coli (ecldh) to gain an insi ... | 2014 | 25401076 |
| action of essential oils from brazilian native and exotic medicinal species on oral biofilms. | essential oils (eo) obtained from twenty medicinal and aromatic plants were evaluated for their antimicrobial activity against the oral pathogens candida albicans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis and streptococcus mitis. | 2014 | 25407737 |
| shifts in campylobacter species abundance may reflect general microbial community shifts in periodontitis progression. | oral campylobacter species have been found to be associated with periodontitis progression. while the etiological significance of campylobacter rectus is quite established, the association of c. gracilis, c. concisus, and c. curvus with health or disease remains contradictory. | 2014 | 25412608 |
| detection of periodontal pathogens in the patients with aortic aneurysm. | the occurrence and development of aortic aneurysm (aa) are associated with infection. some researchers have detected the dna of periodontal pathogens in aa samples in certain populations. however, it has not been done in chinese population. the objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of aa patients. | 2014 | 25430459 |
| regulation of adrenomedullin and nitric oxide production by periodontal bacteria. | in periodontitis the host response to bacterial challenge includes activity of the multifunctional molecules adrenomedullin (am) and nitric oxide (no). the aim of this study was to investigate the role of periodontal bacteria in regulating the production of these molecules from cultured cells. | 2015 | 25440112 |
| antimicrobial and immunomodulatory properties of pgla-am1, cpf-am1, and magainin-am1: potent activity against oral pathogens. | cationic amphipathic α-helical peptides are intensively studied classes of host defence peptides (hdps). three peptides, peptide glycine-leucine-amide (pgla-am1), caerulein-precursor fragment (cpf-am1) and magainin-am1, originally isolated from norepinephrine-stimulated skin secretions of the african volcano frog xenopus amieti (pipidae), were studied for their antimicrobial and immunomodulatory activities against oral and respiratory pathogens. minimal effective concentrations (mecs), determine ... | 2014 | 25447193 |
| term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery. | infection is considered a possible trigger for preterm labour, supported by evidence showing the presence of bacteria in the placenta and placental membranes from preterm births. in this study, 16s rdna pyrosequencing was used to identify bacteria in placental membranes. caesarean sections and vaginal deliveries at term were found to harbour common genera. mycoplasma hominis, aerococcus christensenii, gardnerella vaginalis and fusobacterium nucleatum were either only present in preterm membranes ... | 2014 | 25458966 |
| oral gram-negative anaerobic bacilli as a reservoir of β-lactam resistance genes facilitating infections with multiresistant bacteria. | many β-lactamases have been described in various gram-negative bacilli (capnocytophaga, prevotella, fusobacterium, etc.) of the oral cavity, belonging to class a of the ambler classification (cepa, cbla, cfxa, csp-1 and tem), class b (cfia) or class d in fusobacterium nucleatum (fus-1). the minimum inhibitory concentrations of β-lactams are variable and this variation is often related to the presence of plasmids or other mobile genetic elements (mges) that modulate the expression of resistance g ... | 2015 | 25465519 |
| energetics of pathogenic bacteria and opportunities for drug development. | the emergence and spread of drug-resistant pathogens and our inability to develop new antimicrobials to overcome resistance has inspired scientists to consider new targets for drug development. cellular bioenergetics is an area showing promise for the development of new antimicrobials, particularly in the discovery of new anti-tuberculosis drugs where several new compounds have entered clinical trials. in this review, we have examined the bioenergetics of various bacterial pathogens, highlightin ... | 2014 | 25476763 |
| distribution of 8 periodontal microorganisms in family members of chinese patients with aggressive periodontitis. | to date, no information on the distribution of periodontal microorganisms among family members of chinese patients with aggressive peridontitis (agp) is available. the aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members. | 2015 | 25526624 |
| myopericarditis associated with fusobacterium nucleatum-caused liver abscess. | a wide clinical spectrum of bacteremic disease caused by fusobacterium has been presented in this journal. we wish to extend this spectrum by presenting a case of myopericarditis resulting from a liver abscess caused by f. nucleatum. while f. nucleatum plays an important role in periodontal disease, and has been isolated from skin ulcers, liver abscesses, urinary tract infections, and endocarditis, a single case of f. nucleatum-induced pericarditis is documented in the literature. | 2015 | 25530149 |
| microbial leakage at the implant-abutment connection due to implant insertion maneuvers: cross-sectional study 5 years postloading in healthy patients. | the aim of this study was to test if stress on the prosthetic connection during insertion maneuvers can induce micro-warping at the implant connection. from september 2011 to july 2013, patients with implants loaded for at least 5 years that were placed with 2 different insertion implant mounters-mp (conventional) and me (mountless)-were selected from all of those who had received dental implant therapy in the past and were attending routine check-up or spontaneous visits during the study period ... | 2015 | 25536224 |
| fap2 of fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth. | fusobacterium nucleatum is a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. in the oral environment, f. nucleatum adheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dental biofilm. many of these interactions (called coadherences or coaggregations) are galactose sensitive. galactose-sensitive interactions are also involved in the binding of f. nucle ... | 2015 | 25561710 |
| n-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice. | we previously reported that human serum significantly reduces the invasion of various oral bacterial species into gingival epithelial cells in vitro. the aims of the present study were to characterize the serum component(s) responsible for the inhibition of bacterial invasion of epithelial cells and to examine their effect on periodontitis induced in mice. | 2014 | 25568806 |
| effects of adjunctive daily phototherapy on chronic periodontitis: a randomized single-blind controlled trial. | the purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (pt) as an adjunct to scaling and root planing (srp) in patients with chronic periodontitis. | 2014 | 25568808 |
| fusobacterium nucleatum: an emerging bug in colorectal tumorigenesis. | the human intestinal microbiota is a plethora of diverse microbial species, wherein certain bacteria considered as driver bacteria with procarcinogenic features contribute directly toward colonic epithelium cell damage to initiate colorectal carcinogenesis. however, some bacteria, in particular fusobacterium nucleatum, which is otherwise a normal resident of the oral microflora and a relatively poor colonizer of the healthy gut, have also been considered to play a role in the development of colo ... | 2015 | 25569450 |
| one-step purification and porin transport activity of the major outer membrane proteins p2 from haemophilus influenzae, foma from fusobacterium nucleatum and porb from neisseria meningitidis. | bacterial porins are major outer membrane proteins that function as essential solute transporters between the bacteria and the extracellular environment. structural features of porins are also recognized by eukaryotic cell receptors involved in innate and adaptive immunity. to better investigate the function of porins, proper refolding is necessary following purification from inclusion bodies [1, 2]. using a single-step size exclusion chromatographic method, we have purified three major porins f ... | 2015 | 25575589 |
| fusobacterium nucleatum: a commensal-turned pathogen. | fusobacterium nucleatum is an anaerobic oral commensal and a periodontal pathogen associated with a wide spectrum of human diseases. this article reviews its implication in adverse pregnancy outcomes (chorioamnionitis, preterm birth, stillbirth, neonatal sepsis, preeclampsia), gi disorders (colorectal cancer, inflammatory bowel disease, appendicitis), cardiovascular disease, rheumatoid arthritis, respiratory tract infections, lemierre's syndrome and alzheimer's disease. the virulence mechanisms ... | 2015 | 25576662 |
| microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles. | nanoparticulate silver has recently been reported as an effective antimicrobial agent. the aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. all the participants received, in a crossover format, the following toothbrushing interventions: (i ... | 2015 | 25581046 |
| fusobacterium and escherichia: models of colorectal cancer driven by microbiota and the utility of microbiota in colorectal cancer screening. | intestinal microbiota has emerging roles in the development of colorectal cancer (crc). intestinal dysbiosis, with altered levels of specific bacteria, is consistently seen in crc. the heart of the debate lies in whether these bacteria are a cause or consequence of crc. two bacteria in particular, fusobacterium nucleatum and escherichia coli, have consistently been associated with crc. this review will examine evidence supporting oncogenic roles of f. nucleatum and e. coli. the proposed mechanis ... | 2015 | 25582922 |
| selected dietary (poly)phenols inhibit periodontal pathogen growth and biofilm formation. | periodontitis (pd) is a chronic infectious disease mediated by bacteria in the oral cavity. (poly)phenols (pps), ubiquitous in plant foods, possess antimicrobial activities and may be useful in the prevention and management of periodontitis. the objective of this study was to test the antibacterial effects of selected pps on periodontal pathogens, on both planktonic and biofilm modes of growth. selected pps (n = 48) were screened against streptococcus mitis (s. mitis), aggregatibacter actinomyce ... | 2015 | 25585200 |
| fusobacterium nucleatum endocarditis presenting as liver and brain abscesses in an immunocompetent patient. | 2015 | 25588024 | |
| association between periodontal condition and subgingival microbiota in women during pregnancy: a longitudinal study. | in this study, the gingival conditions and the quantitative detection for aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis and prevotella intermedia in pregnant women were determined. | 2014 | 25591021 |
| anti-inflammatory, antioxidant and antimicrobial effects of artemisinin extracts from artemisia annua l. | the anti-inflammatory, antioxidant, and antimicrobial properties of artemisinin derived from water, methanol, ethanol, or acetone extracts of artemisia annua l. were evaluated. all 4 artemisinin-containing extracts had anti-inflammatory effects. of these, the acetone extract had the greatest inhibitory effect on lipopolysaccharide-induced nitric oxide (no), prostaglandin e2 (pge2), and proinflammatory cytokine (il-1β , il-6, and il-10) production. antioxidant activity evaluations revealed that t ... | 2015 | 25605993 |
| [research progress in fada from fusobacterium nucleatum]. | 2014 | 25619301 | |
| 16s rrna based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis. | dna microarray analysis is a computer based technology, that a reverse capture, which targets 10 periodontal bacteria (parocheck) is available for rapid semi-quantitative determination. the aim of this three-year retrospective study was to display the microarray analysis results for the subgingival biofilm samples taken from patient cases diagnosed with different forms of periodontitis. a total of 84 patients with generalized aggressive periodontitis (gap,n:29), generalized chronic periodontitis ... | 2015 | 25638399 |