PMID(sorted descending)
arginine-ornithine antiporter arcd controls arginine metabolism and interspecies biofilm development of streptococcus gordonii.arginine is utilized by the oral inhabitant streptococcus gordonii as a substrate of the arginine deiminase system (ads), eventually producing atp and nh3, the latter of which is responsible for microbial resistance to ph stress. s. gordonii expresses a putative arginine-ornithine antiporter (arcd) whose function has not been investigated despite relevance to the ads and potential influence on inter-bacterial communication with periodontal pathogens that utilize amino acids as a main energy sour ...201526085091
influence of biofilm formation by gardnerella vaginalis and other anaerobes on bacterial vaginosis.bacterial vaginosis (bv) is the worldwide leading vaginal disorder among women of reproductive age. bv is characterized by the replacement of beneficial lactobacilli and the augmentation of anaerobic bacteria. gardnerella vaginalis is a predominant bacterial species, but bv is also associated with other numerous anaerobes, such as atopobium vaginae, mobiluncus mulieris, prevotella bivia, fusobacterium nucleatum, and peptoniphilus species. currently, the role of g. vaginalis in the etiology of bv ...201526080369
fusobacterium nucleatum alters atherosclerosis risk factors and enhances inflammatory markers with an atheroprotective immune response in apoe(null) mice.the american heart association supports an association between periodontal disease (pd) and atherosclerotic vascular disease (asvd) but does not as of yet support a causal relationship. recently, we have shown that major periodontal pathogens porphyromonas gingivalis and treponema denticola are causally associated with acceleration of aortic atherosclerosis in apoenull hyperlipidemic mice. the aim of this study was to determine if oral infection with another significant periodontal pathogen fuso ...201526079509
influence of the prosthetic index into morse taper implants on bacterial evaluate the influence of morse taper implant index on microleakage.201526068320
alcohol consumption and periodontitis: quantification of periodontal pathogens and cytokines.there are few studies on periodontal status related to microbiologic and immunologic profiles among individuals not or occasionally using alcohol and those with alcohol dependence. the aim of this study is to determine the effect of alcohol consumption on the levels of subgingival periodontal pathogens and proinflammatory cytokines (interleukin [il]-1β and tumor necrosis factor [tnf]-α) in the gingival fluid among individuals with and without periodontitis.201526062839
microflora in oral ecosystems and salivary secretion rates--a 3-year follow-up after radiation therapy to the head and neck region.results indicate that late improvements of radiation therapy (rt) in the head and neck region may diminish the long-term effects on salivary glands and oral microflora. the aim was therefore to analyze salivary secretion rates and oral microflora over time in rt subjects.201526058004
peripheral blood neutrophil cytokine hyper-reactivity in chronic cytokine release (il-8, il-6, tnf-α, il-1β) by peripheral blood neutrophils, isolated from periodontitis patients (before/after therapy) and matched controls, was determined after 18 h culture in the presence/absence of escherichia coli lps, opsonised staphylococcus aureus, heat-killed fusobacterium nucleatum and porphyromonas gingivalis. all cultures demonstrated differences in the amounts of each cytokine detected (p < 0.0001), with a clear release pattern (il-8 > il-6 > tnf-α ...201526055820
mechanistic analysis of plga/hpmc-based in-situ forming implants for periodontitis forming implant formulations based on poly(lactic-co-glycolic acid) (plga), acetyltributyl citrate (atbc), minocycline hcl, n-methyl pyrrolidone (nmp) and optionally hydroxypropyl methylcellulose (hpmc) were prepared and thoroughly characterized in vitro. this includes electron paramagnetic resonance (epr), nuclear magnetic resonance ((1)h nmr), mass change and drug release measurements under different conditions, optical microscopy, size exclusion chromatography (sec) as well as antibac ...201526047797
microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model.the development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth-associated biofilm. this in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a 'supragingival' aerobic profile to a 'subgingival' anaerobic profile. biofilms consisting of actinomyces oris, candida albicans, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar were aerobically grown in saliva ...201626033167
[a case of corticosteroid-responsive lemierre syndrome with clivus osteomyelitis and a mass in the cavernous sinus-suprasellar region].lemierre syndrome is a clinical syndrome that presents with internal jugular thrombophlebitis, septicemia and systemic abscess formations. in general, the condition is preceded by oropharyngeal infections. we report a case of a 73-year-old man with lemierre syndrome, clivus osteomyelitis and a steroid-responsive mass in the cavernous sinus-suprasellar region. he complained of fever, occipital pain, diplopia and right ptosis. administration of oral steroids ameliorated the ophthalmic symptoms for ...201526028195
coaggregation and biofilm growth of granulicatella spp. with fusobacterium nucleatum and aggregatibacter actinomycetemcomitans.members of fastidious granulicatella and aggregatibacter genera belong to normal oral flora bacteria that can cause serious infections, such as infective endocarditis. aggregatibacter actinomycetemcomitans has long been implicated in aggressive periodontitis, whereas dna-based methods only recently showed an association between granulicatella spp. and dental diseases. as bacterial coaggregation is a key phenomenon in the development of oral and nonoral multispecies bacterial communities it would ...201526025449
[spondylodiscitis due to fusobacterium nucleatum: new diagnostic method].fusobacterium spp. are gram negative anaerobe bacteria. vertebral osteomyelitis caused by these bacteria is very unusual; in fact, we could only find 11 cases in the literature. we report the case of a male, 46 year-old patient who had had lumbar pain for several weeks that irradiated to the right leg, and did not respond to nsaid treatment. the work-up included mri, biopsy with draining of the collection and a universal pcr followed by 16s rdna sequencing. the latter was used to make the microb ...201526021107
a clinical study evaluating changes in the microbial flora around dental implants during various stages of implant restoration.this study evaluated the microbial colonization of dental implants in healthy patients before placing the implants and during the various stages of implant treatment.201525996787
real-time monitoring of the metabolic activity of periodontopathic bacteria.bacterial metabolic activity is associated with the onset and progression mechanisms of oral biofilm-mediated disease; however, at present no method to monitor bacterial metabolism exists, especially for periodontopathic bacteria. therefore, we aimed to establish a novel method for monitoring the metabolic activity of periodontopathic bacteria, porphyromonas gingivalis (pg), prevotella intermedia (pi) and fusobacterium nucleatum (fn), as well as streptococcus mutans (sm) for comparison. the meth ...201525986950
liquid based microbiological transport systems: conformity assessment of two commercial devices.we compared two types of liquid-based microbiology devices for microorganism viability according to standardized quantitative elution method clsi m40-a2. the eswab® met clsi acceptance criteria of viability maintenance for all microorganisms tested. the σ-transwab® failed to meet clsi acceptance criteria for peptostreptococcus anaerobius, prevotella melaninogenica, fusobacterium nucleatum and haemophilus influenzae.201525957512
internal jugular vein septic thrombophlebitis (lemierre syndrome) as a complication of pharyngitis.sore throat is a common presenting complaint in the outpatient setting. most cases are nonbacterial in origin, but those that are bacterial are usually the result of group a β-hemolytic streptococcus. guidelines exist to help physicians decide whether to treat with an antibiotic. lemierre syndrome is a dangerous potential sequela of pharyngitis that results in septic thrombophlebitis of the internal jugular (ij) vein. a high index of suspicion is needed to consider this diagnosis in the workup o ...201525957375
extensive identification of bacterial riboflavin transporters and their distribution across bacterial species.riboflavin, the precursor for the cofactors flavin mononucleotide (fmn) and flavin adenine dinucleotide, is an essential metabolite in all organisms. while the functions for de novo riboflavin biosynthesis and riboflavin import may coexist in bacteria, the extent of this co-occurrence is undetermined. the ribm, ribn, rfuabcd and the energy-coupling factor-ribu bacterial riboflavin transporters have been experimentally characterized. in addition, impx, rfnt and ribxy are proposed as riboflavin tr ...201525938806
infections caused by fusobacterium in children: a 14-year single-center experience.this study aimed at reviewing our experience with infections caused by fusobacterium in children.201525929419
development of in vitro denture biofilm models for halitosis related bacteria and their application in testing the efficacy of antimicrobial agents.since dentures can serve as a reservoir for halitosis-causing oral bacteria, halitosis development is a concern for denture wearers. in this study, we surveyed the prevalence of four selected halitosis-related species (fusobacterium nucleatum, tannerella forsythia, veillonella atypica and klebsiella pneumoniae) in clinical denture plaque samples, and developed denture biofilm models for these species in vitro to facilitate assessment of antimicrobial treatment efficacy. design : denture plaque f ...201525926895
quantitation of biofilm and planktonic life forms of coexisting periodontal species.complexity of oral polymicrobial communities has prompted a need for developing in vitro models to study behavior of coexisting bacteria. little knowledge is available of in vitro co-growth of several periodontitis-associated species without early colonizers of dental plaque.201525926392
pyrosequencing analysis of subgingival microbiota in distinct periodontal conditions.subgingival microorganisms are potentially associated with periodontal diseases. however, changes in the subgingival microbiota during the progress of periodontal diseases are poorly understood. in this study, we analyzed bacterial communities in the subgingival paper point samples from 32 korean individuals with no sign of disease, gingivitis, or periodontitis using 454 flx titanium pyrosequencing. a total of 256,113 reads representing 26 phyla, 433 genera, and 1,016 species were detected. bact ...201525904141
influence of complement on neutrophil extracellular trap release induced by bacteria.neutrophil extracellular trap (net) release has generally been studied in the absence of serum, or at low concentrations of untreated or heat-inactivated serum. the influence of serum complement on net release therefore remains unclear. we examined the dna release induced by staphylococcus aureus and three oral bacteria: actinomyces viscosus, aggregatibacter actinomycetemcomitans and fusobacterium nucleatum subsp. vincettii.201625900429
microbiological profile resistant to different intracanal medications in primary endodontic infections.this clinical study aimed to determine the microbiological profile resistant to different intracanal medications in primary endodontic infections by using both microbiological culture and the checkerboard dna-dna hybridization technique.201525892513
bacterial profile of aggressive periodontitis in morocco: a cross-sectional study.aggressive periodontitis (agp) is one of the most severe forms of periodontal diseases. in morocco, aggregatibacter actinomycetemcomitans has been strongly associated with agp, however limited knowledge is available about the implication of other periodontal pathogens in this entity. therefore, the main aim of this study was to evaluate the composition of the subgingival microbiota in moroccan patients with agp.201525888404
laser supported reduction of specific microorganisms in the periodontal pocket with the aid of an er,cr:ysgg laser: a pilot study.the aim of this study was to evaluate the effectiveness of a radial firing tip of an er,cr:ysgg laser as an adjunct to a nonsurgical periodontal treatment.201525879057
antimicrobial activity of chemokine cxcl10 for dermal and oral microorganisms.cxcl10 (ip-10) is a small 10 kda chemokine with antimicrobial activity. it is induced by ifn-γ, chemoattracts mononuclear cells, and promotes adhesion of t cells. recently, we detected cxcl10 on the surface of the skin and in the oral cavity. in the current study, we used broth microdilution and radial diffusion assays to show that cxcl10 inhibits the growth of escherichia coli, staphylococcus aureus, corynebacterium jeikeium, corynebacterium striatum, and candida albicans hmv4c, but not coryneb ...201425859394
occurrence of periodontal pathogens in ethnic groups from a native brazilian reservation.the present study was designed to evaluate the occurrence of periodontal pathogens in the subgingival biofilm of 100 native brazilians living at the umutina indian reservation, mato grosso state, brazil.201525847068
pyogenic liver abscess caused by fusobacterium in a 21-year-old immunocompetent male.a 21-year-old male with no significant past medical history, presented with right upper quadrant (ruq) abdominal pain along with fevers and chills. lab work revealed leukocytosis, anemia, and slightly elevated alkaline phosphatase. viral serology for hepatitis b virus, hepatitis c virus, and human immunodeficiency virus were negative and he was immunocompetent. computed tomography imaging revealed hepatic abscesses, the largest measuring 9.5 cm. empiric antibiotics were started and percutaneous ...201525834342
microbiota at teeth and implants in partially edentulous patients. a 10-year retrospective determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface.201625827437
[features of adhesion of anaerobic periodontopathogenic bacteria and candida albicans fungi to experimental samples of basis dental plastic depending on surface roughness and polishing method].study the main surface parameters of milled polyacrylic materials using atomic force microscopy and primary microbial adhesion of periodontopathogenic group bacteria and candida albicans fungi taking into consideration the method of sample polishing.201625816509
prelude to oral microbes and chronic diseases: past, present and future.associations between oral and systemic health are ancient. oral opportunistic bacteria, particularly, porphyromonas gingivalis and fusobacterium nucleatum, have recently been deviated from their traditional roles as periodontal pathogens and arguably ascended to central players based on their participations in complex co-dependent mechanisms of diverse systemic chronic diseases risk and pathogenesis, including cancers, rheumatoid-arthritis, and diabetes.201525813714
abnormal pregnancy outcomes in mice using an induced periodontitis model and the haematogenous migration of fusobacterium nucleatum sub-species to the murine investigate if there is subspecies specific migration to the placenta by fusobacterium nucleatum (fn) and to determine whether experimentally induced periodontitis results in adverse pregnancy outcomes (apo) in mice.201525806806
short-chain fatty acids in infected root canals of teeth with apical periodontitis before and after treatment.short-chain fatty acids (scfas) are bacterial metabolic end products that may function as virulence factors. this study evaluated the occurrence of scfas in infected root canals before and after treatment.201525799538
the effect of blue light on periodontal biofilm growth in vitro.we have previously shown that blue light eliminates the black-pigmented oral bacteria porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens, and prevotella melaninogenica. in the present study, the in vitro photosensitivity of the above black-pigmented microorganisms and four fusobacteria species (fusobacterium nucleatum ss. nucleatum, f. nucleatum ss. vincentii, f. nucleatum ss. polymorphum, fusobacterium periodonticum) was investigated in pure cultures and human dental plaque ...201525759232
antibacterial photodynamic treatment of periodontopathogenic bacteria with indocyanine green and near-infrared laser light enhanced by trolox(tm).it has been shown that certain vitamins can significantly enhance the effect of photodynamic anti-tumor therapy. unfortunately, there is no sufficient information available about the impact of those antioxidants on antimicrobial photodynamic therapy (apdt). the present study is aimed at investigating the antimicrobial effect of the dye indocyanine green (icg) in the presence of trolox(tm) , a vitamin e analogue, upon irradiation with near-infrared (nir) laser light (808 nm) on the gramnegative p ...201525753989
subacute osteomyelitis of the femur due to fusobacterium nucleatum in a 7-year-old boy.subacute hematogenous osteomyelitis is an insidious infection, which commonly has a delayed diagnosis. we describe the case of a 7-year-old boy with subacute osteomyelitis, which was initially considered to be a bone tumor. infection should be considered in all cases of bone pain, especially in children, even in the absence of typical systemic features of inflammation.201525742081
dna methylation differentially regulates cytokine secretion in gingival epithelia in response to bacterial challenges.epigenetic modifications are changes in gene expression without altering dna sequence. we previously reported that bacteria-specific innate immune responses are regulated by epigenetic modifications. our hypothesis is that dna methylation affects gingival cytokine secretion in response to bacterial stimulation. gingival epithelial cells (gecs) were treated with dnmt-1 inhibitors prior to porphyromonas gingivalis (pg) or fusobacterium nucleatum (fn) exposure. protein secretion was assessed using ...201525722484
oral bacteria adherence to suture threads: an in vitro study.this study investigated the adherence of oral microorganisms to different types of suture threads.201525711725
high in vitro antibacterial activity of pac-525 against porphyromonas gingivalis biofilms cultured on order to investigate the potential of short antimicrobial peptides (amps) as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short amps, that is, pac-525, ksl-w, and ksl, was determined using the mtt assay. the antimicrobial activity of these amps, ranging in concentration from 0.0039 mg/ml to 0.5 mg/ml, against the predominant planktonic pathogens, including streptococcus sanguis, fusobacterium nucleatum, and porphyromonas gingivalis ...201525710035
killing dental pathogens using antibacterial graphene caries and periodontal diseases have a close relationship with microbes such as streptococcus mutans, porphyromonas gingivalis and fusobacterium nucleatum. graphene oxide (go), as the derivative of graphene, plays an important role in many areas including biology and medicine. in particular, it has been known as a promising antimicrobial nanomaterial. in this study, we focused on the antimicrobial property of go against dental pathogens. with the utilization of 3-(4,5-dimethylthiazol-2-yl ...201525705785
bactericidal effect of visible light in the presence of erythrosine on porphyromonas gingivalis and fusobacterium nucleatum compared with diode laser, an in vitro study.recently, photodynamic therapy (pdt) has been introduced as a new modality in oral bacterial decontamination. besides, the ability of laser irradiation in the presence of photosensitizing agent to lethal effect on oral bacteria is well documented. current research aims to evaluate the effect of photodynamic killing of visible blue light in the presence of plaque disclosing agent erythrosine as photosensitizer on porphyromonas gingivalis associated with periodontal bone loss and fusobacterium nuc ...201425705082
association of fusobacterium nucleatum with clinical and molecular features in colorectal serrated pathway.human gut microbiota is being increasingly recognized as a player in colorectal cancers (crcs). evidence suggests that fusobacterium nucleatum (f. nucleatum) may contribute to disease progression and is associated with cpg island methylator phenotype (cimp) and microsatellite instability (msi) in crcs; however, to date, there are no reports about the relationship between f. nucleatum and molecular features in the early stage of colorectal tumorigenesis. therefore, we investigated the presence of ...201525703934
an oral commensal associates with disease: chicken, egg, or red herring?fusobacterium nucleatum might be the cause or consequence of disease in many tissues in and outside the mouth. in this issue of immunity, gur et al. (2015) suggest a new mechanism by which this oral commensal might help cancer cells escape host immunity.201525692696
binding of the fap2 protein of fusobacterium nucleatum to human inhibitory receptor tigit protects tumors from immune cell attack.bacteria, such as fusobacterium nucleatum, are present in the tumor microenvironment. however, the immunological consequences of intra-tumoral bacteria remain unclear. here, we have shown that natural killer (nk) cell killing of various tumors is inhibited in the presence of various f. nucleatum strains. our data support that this f. nucleatum-mediated inhibition is mediated by human, but not by mouse tigit, an inhibitory receptor present on all human nk cells and on various t cells. using a lib ...201525680274
evaluation of holy basil mouthwash as an adjunctive plaque control agent in a four day plaque regrowth model.various antibacterial and antiplaque agents are used in chemical plaque control but none are without their shortcomings. chlorhexidine considered a gold standard, also has an array of side effects. to overcome these, numerous herbal extracts have been tried and tested and one among them is holy basil. the present study evaluated the antibacterial efficacy of holy basil in vitro against some periodontopathogens and its antiplaque effect in vivo.201425674314
[cerebellar abscess due to infection with the anaerobic bacteria fusobacterium nucleatum: a case report].we report a rare case of cerebellar abscess produced by anaerobic bacteria. a 76-year-old man was admitted to our hospital with a history of fever, vomiting, and dizziness lasting 14 days. computed tomography(ct)scan and magnetic resonance images showed the presence of a multiloculated cerebellar abscess with a right subdural abscess. the patient underwent aspiration of the abscess through a suboccipital craniotomy. fusobacterium nucleatum, which is an anaerobic bacteria naturally present in the ...201525672555
high individuality of respiratory bacterial communities in a large cohort of adult cystic fibrosis patients under continuous antibiotic treatment.routine clinical diagnostics of cf patients focus only on a restricted set of well-known pathogenic species. recent molecular studies suggest that infections could be polymicrobial with many bacteria not detected by culture-based diagnostics.201525671713
[antibacterial activity of synthetic antimicrobial decapeptide against oral bacteria].this study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. methods in this study, we investigated the antimicrobial activity of decapeptide against streptococcus mutans (s. mutans), streptococcus sobrinus, lactobacillus acidophilus, streptococcus sanguis, streptococcus gordonii, actinomyces viscosus, actinomyces naeslundii, porphyromonas gingivalis, prevotella intermedia, fusob ...201425665430
changes in microflora in dental plaque from cancer patients undergoing chemotherapy and the relationship of these changes with mucositis: a pilot assess changes in oral microflora in dental plaque from cancer patients within 7 days of the first course of chemotherapy, and the relationship of the changes with mucositis.201525662538
novel therapeutic approach for the treatment of periodontitis by curcumin.the aim of the present study was to evaluate the clinical and microbiological efficacy of locally delivered 1% curcumin gel as an adjunct to scaling and root planing in the treatment of chronic periodontitis.201425654035
fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry.we explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) for identification of fusobacterium nucleatum subspecies. maldi-tof ms spectra of five f. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. maldi-tof ms with the modified saramis database further correctly identified 28 of ...201525653408
isolation of alkaline-tolerant bacteria from primary infected root canals.alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. the aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (s1), after chemomechanical root canal preparation (s2), and after calcium hydroxide dressing (s3).201525638530
16s rrna based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis.dna microarray analysis is a computer based technology, that a reverse capture, which targets 10 periodontal bacteria (parocheck) is available for rapid semi-quantitative determination. the aim of this three-year retrospective study was to display the microarray analysis results for the subgingival biofilm samples taken from patient cases diagnosed with different forms of periodontitis. a total of 84 patients with generalized aggressive periodontitis (gap,n:29), generalized chronic periodontitis ...201525638399
[research progress in fada from fusobacterium nucleatum]. 201425619301
anti-inflammatory, antioxidant and antimicrobial effects of artemisinin extracts from artemisia annua l.the anti-inflammatory, antioxidant, and antimicrobial properties of artemisinin derived from water, methanol, ethanol, or acetone extracts of artemisia annua l. were evaluated. all 4 artemisinin-containing extracts had anti-inflammatory effects. of these, the acetone extract had the greatest inhibitory effect on lipopolysaccharide-induced nitric oxide (no), prostaglandin e2 (pge2), and proinflammatory cytokine (il-1β , il-6, and il-10) production. antioxidant activity evaluations revealed that t ...201525605993
association between periodontal condition and subgingival microbiota in women during pregnancy: a longitudinal this study, the gingival conditions and the quantitative detection for aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis and prevotella intermedia in pregnant women were determined.201425591021
fusobacterium nucleatum endocarditis presenting as liver and brain abscesses in an immunocompetent patient. 201525588024
selected dietary (poly)phenols inhibit periodontal pathogen growth and biofilm formation.periodontitis (pd) is a chronic infectious disease mediated by bacteria in the oral cavity. (poly)phenols (pps), ubiquitous in plant foods, possess antimicrobial activities and may be useful in the prevention and management of periodontitis. the objective of this study was to test the antibacterial effects of selected pps on periodontal pathogens, on both planktonic and biofilm modes of growth. selected pps (n = 48) were screened against streptococcus mitis (s. mitis), aggregatibacter actinomyce ...201525585200
fusobacterium and escherichia: models of colorectal cancer driven by microbiota and the utility of microbiota in colorectal cancer screening.intestinal microbiota has emerging roles in the development of colorectal cancer (crc). intestinal dysbiosis, with altered levels of specific bacteria, is consistently seen in crc. the heart of the debate lies in whether these bacteria are a cause or consequence of crc. two bacteria in particular, fusobacterium nucleatum and escherichia coli, have consistently been associated with crc. this review will examine evidence supporting oncogenic roles of f. nucleatum and e. coli. the proposed mechanis ...201525582922
microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles.nanoparticulate silver has recently been reported as an effective antimicrobial agent. the aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. all the participants received, in a crossover format, the following toothbrushing interventions: (i ...201525581046
fusobacterium nucleatum: a commensal-turned pathogen.fusobacterium nucleatum is an anaerobic oral commensal and a periodontal pathogen associated with a wide spectrum of human diseases. this article reviews its implication in adverse pregnancy outcomes (chorioamnionitis, preterm birth, stillbirth, neonatal sepsis, preeclampsia), gi disorders (colorectal cancer, inflammatory bowel disease, appendicitis), cardiovascular disease, rheumatoid arthritis, respiratory tract infections, lemierre's syndrome and alzheimer's disease. the virulence mechanisms ...201525576662
one-step purification and porin transport activity of the major outer membrane proteins p2 from haemophilus influenzae, foma from fusobacterium nucleatum and porb from neisseria meningitidis.bacterial porins are major outer membrane proteins that function as essential solute transporters between the bacteria and the extracellular environment. structural features of porins are also recognized by eukaryotic cell receptors involved in innate and adaptive immunity. to better investigate the function of porins, proper refolding is necessary following purification from inclusion bodies [1, 2]. using a single-step size exclusion chromatographic method, we have purified three major porins f ...201525575589
fusobacterium nucleatum: an emerging bug in colorectal tumorigenesis.the human intestinal microbiota is a plethora of diverse microbial species, wherein certain bacteria considered as driver bacteria with procarcinogenic features contribute directly toward colonic epithelium cell damage to initiate colorectal carcinogenesis. however, some bacteria, in particular fusobacterium nucleatum, which is otherwise a normal resident of the oral microflora and a relatively poor colonizer of the healthy gut, have also been considered to play a role in the development of colo ...201525569450
effects of adjunctive daily phototherapy on chronic periodontitis: a randomized single-blind controlled trial.the purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (pt) as an adjunct to scaling and root planing (srp) in patients with chronic periodontitis.201425568808
n-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice.we previously reported that human serum significantly reduces the invasion of various oral bacterial species into gingival epithelial cells in vitro. the aims of the present study were to characterize the serum component(s) responsible for the inhibition of bacterial invasion of epithelial cells and to examine their effect on periodontitis induced in mice.201425568806
fap2 of fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth.fusobacterium nucleatum is a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. in the oral environment, f. nucleatum adheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dental biofilm. many of these interactions (called coadherences or coaggregations) are galactose sensitive. galactose-sensitive interactions are also involved in the binding of f. nucle ...201525561710
microbial leakage at the implant-abutment connection due to implant insertion maneuvers: cross-sectional study 5 years postloading in healthy patients.the aim of this study was to test if stress on the prosthetic connection during insertion maneuvers can induce micro-warping at the implant connection. from september 2011 to july 2013, patients with implants loaded for at least 5 years that were placed with 2 different insertion implant mounters-mp (conventional) and me (mountless)-were selected from all of those who had received dental implant therapy in the past and were attending routine check-up or spontaneous visits during the study period ...201525536224
myopericarditis associated with fusobacterium nucleatum-caused liver abscess.a wide clinical spectrum of bacteremic disease caused by fusobacterium has been presented in this journal. we wish to extend this spectrum by presenting a case of myopericarditis resulting from a liver abscess caused by f. nucleatum. while f. nucleatum plays an important role in periodontal disease, and has been isolated from skin ulcers, liver abscesses, urinary tract infections, and endocarditis, a single case of f. nucleatum-induced pericarditis is documented in the literature.201525530149
distribution of 8 periodontal microorganisms in family members of chinese patients with aggressive date, no information on the distribution of periodontal microorganisms among family members of chinese patients with aggressive peridontitis (agp) is available. the aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members.201525526624
energetics of pathogenic bacteria and opportunities for drug development.the emergence and spread of drug-resistant pathogens and our inability to develop new antimicrobials to overcome resistance has inspired scientists to consider new targets for drug development. cellular bioenergetics is an area showing promise for the development of new antimicrobials, particularly in the discovery of new anti-tuberculosis drugs where several new compounds have entered clinical trials. in this review, we have examined the bioenergetics of various bacterial pathogens, highlightin ...201425476763
oral gram-negative anaerobic bacilli as a reservoir of β-lactam resistance genes facilitating infections with multiresistant bacteria.many β-lactamases have been described in various gram-negative bacilli (capnocytophaga, prevotella, fusobacterium, etc.) of the oral cavity, belonging to class a of the ambler classification (cepa, cbla, cfxa, csp-1 and tem), class b (cfia) or class d in fusobacterium nucleatum (fus-1). the minimum inhibitory concentrations of β-lactams are variable and this variation is often related to the presence of plasmids or other mobile genetic elements (mges) that modulate the expression of resistance g ...201525465519
term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery.infection is considered a possible trigger for preterm labour, supported by evidence showing the presence of bacteria in the placenta and placental membranes from preterm births. in this study, 16s rdna pyrosequencing was used to identify bacteria in placental membranes. caesarean sections and vaginal deliveries at term were found to harbour common genera. mycoplasma hominis, aerococcus christensenii, gardnerella vaginalis and fusobacterium nucleatum were either only present in preterm membranes ...201425458966
antimicrobial and immunomodulatory properties of pgla-am1, cpf-am1, and magainin-am1: potent activity against oral pathogens.cationic amphipathic α-helical peptides are intensively studied classes of host defence peptides (hdps). three peptides, peptide glycine-leucine-amide (pgla-am1), caerulein-precursor fragment (cpf-am1) and magainin-am1, originally isolated from norepinephrine-stimulated skin secretions of the african volcano frog xenopus amieti (pipidae), were studied for their antimicrobial and immunomodulatory activities against oral and respiratory pathogens. minimal effective concentrations (mecs), determine ...201425447193
regulation of adrenomedullin and nitric oxide production by periodontal periodontitis the host response to bacterial challenge includes activity of the multifunctional molecules adrenomedullin (am) and nitric oxide (no). the aim of this study was to investigate the role of periodontal bacteria in regulating the production of these molecules from cultured cells.201525440112
detection of periodontal pathogens in the patients with aortic aneurysm.the occurrence and development of aortic aneurysm (aa) are associated with infection. some researchers have detected the dna of periodontal pathogens in aa samples in certain populations. however, it has not been done in chinese population. the objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of aa patients.201425430459
shifts in campylobacter species abundance may reflect general microbial community shifts in periodontitis progression.oral campylobacter species have been found to be associated with periodontitis progression. while the etiological significance of campylobacter rectus is quite established, the association of c. gracilis, c. concisus, and c. curvus with health or disease remains contradictory.201425412608
action of essential oils from brazilian native and exotic medicinal species on oral biofilms.essential oils (eo) obtained from twenty medicinal and aromatic plants were evaluated for their antimicrobial activity against the oral pathogens candida albicans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis and streptococcus mitis.201425407737
diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three gram-negative bacteria.nad-dependent d-lactate dehydrogenases (d-ldhs) reduce pyruvate into d-lactate with oxidation of nadh into nad(+). although non-allosteric d-ldhs from lactobacilli have been extensively studied, the catalytic properties of allosteric d-ldhs from gram-negative bacteria except for escherichia coli remain unknown. we characterized the catalytic properties of d-ldhs from three gram-negative bacteria, fusobacterium nucleatum (fnldh), pseudomonas aeruginosa (paldh), and e. coli (ecldh) to gain an insi ...201425401076
periodontal and peri-implant microbiota in patients with healthy and inflamed periodontal and peri-implant compare the prevalence and levels of six bacterial pathogens within the subgingival/submucosal microbiota at teeth versus implants with various clinical conditions.201625399962
prevalence of six periodontal pathogens in subgingival samples of italian patients with chronic periodontitis.the aim of this study was to investigate the subgingival prevalence of six periodontal pathogens in 352 italian patients with chronic periodontitis. possible correlations with clinical parameters, age, gender and smoking status were also investigated. at first visit a pooled subgingival plaque sample was obtained for each subject by using the paper-point method. the samples were processed and analysed according to a commercially available quantitative real time polymerase chain reaction assay (m ...201425387289
essential oils and isolated compounds from lippia alba leaves and flowers: antimicrobial activity and osteoclast the present study, essential oils extracted from the leaves and flowers of lippia alba (mill.) (l. alba) were analyzed for their antimicrobial activity and their effects on osteoclasts. the periodontal pathogens, aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans; atcc 43717), fusobacterium nucleatum (f. nucleatum; atcc 25586) and porphyromonas gingivalis (p. gingivalis); atcc 33277) were used in antimicrobial activity assays for determining the minimum inhibitory concent ...201525384405
interactions of anaerobic bacteria with dental stem cells: an in vitro patients with periodontitis, it is highly likely that local (progenitor) cells encounter pathogenic bacteria. the purpose of this in vitro study was to elucidate how human dental follicle stem cells (hdfsc) react towards a direct challenge with anaerobic periodontal pathogens under their natural oxygen-free atmosphere. hdfsc were compared to human bone marrow mesenchymal stem cells (hbmsc) and differentiated primary human gingival fibroblasts (hgif), as well as permanent gingival carcinoma ce ...201425369260
identification and characterization of fusolisin, the fusobacterium nucleatum autotransporter serine protease.fusobacterium nucleatum is an oral anaerobe associated with periodontal disease, adverse pregnancy outcomes and colorectal carcinoma. a serine endopeptidase of 61-65 kda capable of damaging host tissue and of inactivating immune effectors was detected previously in f. nucleatum. here we describe the identification of this serine protease, named fusolisin, in three oral f. nucleatum sub-species. gel zymogram revealed fusobacterial proteolytic activity with molecular masses ranging from 55-101 kda ...201425357190
antimicrobial activity of platelet-rich plasma and other plasma preparations against periodontal addition to releasing a pool of growth factors during activation, platelets have many features that indicate their role in the anti-infective host defense. the antimicrobial activities of platelet-rich plasma (prp) and related plasma preparations against periodontal disease-associated bacteria were evaluated.201525345340
effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis.short chain fatty acids (scfas) play important roles in periodontal diseases. however, the concentrations of scfas in gingival crevicular fluid of patients with aggressive periodontitis are not known. the aim of this intervention study was to investigate the influences of non-surgical periodontal therapy on levels of scfas in the gingival crevicular fluid of patients with generalized aggressive periodontitis (g-agp), and analyze the concentrations of scfas in sites with or without the detected p ...201425340203
relationship of clinical and microbiological variables in patients with type 1 diabetes mellitus and periodontitis.the aim of the study was to analyze how metabolic control of type 1 diabetes is related to clinical and microbiological periodontal parameters.201425294115
influence of highly-active antiretroviral therapy on the subgingival biofilm in hiv-infected patients.highly-active antiretroviral therapy (haart) has been associated with alterations in subgingival biofilm and periodontal disease. the purpose of the present study was to investigate the association between different haart regimens and the prevalence of periodontal pathogens in hiv-infected patients with chronic periodontitis.201625283543
subgingival microbiome in smokers and non-smokers in korean chronic periodontitis patients.smoking is a major environmental factor associated with periodontal diseases. however, we still have a very limited understanding of the relationship between smoking and subgingival microflora in the global population. here, we investigated the composition of subgingival bacterial communities from the pooled plaque samples of smokers and non-smokers, 134 samples in each group, in korean patients with moderate chronic periodontitis using 16s rrna gene-based pyrosequencing. a total of 17,927 reads ...201525283067
hydrogen sulfide production from subgingival plaque samples.periodontitis is a polymicrobial anaerobe infection. little is known about the dysbiotic microbiota and the role of bacterial metabolites in the disease process. it is suggested that the production of certain waste products in the proteolytic metabolism may work as markers for disease severity. hydrogen sulfide (h2s) is a gas produced by degradation of proteins in the subgingival pocket. it is highly toxic and believed to have pro-inflammatory properties. we aimed to study h2s production from su ...201525280920
[effects of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions].to investigate the effect of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions.201425263382
effect of bdellovibrio bacteriovorus hd100 on multispecies oral communities.the predation of bdellovibrio bacteriovorus on different periodontal pathogens has already been described. however, it is necessary to consider the polymicrobial nature of periodontal disease. the current study explores the predation of bdellovibrio on oral pathogens organized in multispecies communities. the effect of the predator was evaluated on in vitro six species communities with microbial culturing. additionally, the effect on ex vivo subgingival plaque and saliva samples from periodontit ...201525252124
association between polycystic ovary syndrome, oral microbiota and systemic antibody responses.polycystic ovary syndrome (pcos) is a hormonal disorder of women that not only is the leading cause of infertility but also shows a reciprocal link with oral health. this study aimed to investigate the hypothesis that the levels of putative periodontal pathogens in saliva and their antibody response in serum are elevated in pcos, compared to systemic health. a total of 125 women were included in four groups; 45 women with pcos and healthy periodontium, 35 women with pcos and gingivitis, 25 syste ...201425232962
real-time pcr quantification of six periodontal pathogens in saliva samples from healthy young adults.the use of saliva as a diagnostic fluid for the evaluation of periodontal health has gained attention recently. most published real-time pcr assays focused on quantification of bacteria in subgingival plaque, not in saliva. the aims of this study were to develop a real-time pcr assay for quantification of six periodontal pathogens in saliva and to establish a relationship between the amount of dna (fg) and colony-forming unit (cfu).201525217278
bimix antimicrobial scaffolds for regenerative endodontics.eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. the aim of this study was to synthesize and determine in vitro both the antimicrobial effectiveness and cytocompatibility of bimix antibiotic-containing polydioxanone-based polymer scaffolds.201425201643
the effect of sodium hypochlorite on enterococcus faecalis when grown on dentine as a single- and multi-species biofilm.enterococcus faecalis is often involved in the aetiology of apical periodontitis after endodontic treatment. this project aimed to establish, on dentine in vitro, a multi-species biofilm containing e. faecalis, and to determine if the organism had an increased resistance to sodium hypochlorite compared with an axenic biofilm. biofilms were established on dentine discs in flow cells with either e. faecalis alone (axenic) or together with fusobacterium nucleatum and streptococcus sanguinis. follow ...201425195495
global analysis of saliva as a source of bacterial genes for insights into human population structure and migration studies.the genetic diversity of the human microbiome holds great potential for shedding light on the history of our ancestors. helicobacter pylori is the most prominent example as its analysis allowed a fine-scale resolution of past migration patterns including some that could not be distinguished using human genetic markers. however studies of h. pylori require stomach biopsies, which severely limits the number of samples that can be analysed. by focussing on the house-keeping gene gdh (coding for the ...201425183372
the presence of cariogenic and periodontal pathogens in the oral cavity of one-year-old infants delivered pre-term with very low birthweights: a case control study.recently, the dental literature has focused mainly on the microbial colonization of healthy full-term infants and their mothers or caretakers. however, oral microbial acquisition by premature infants has not been adequately investigated, and the correlation between pre-term birth and the presence of cariogenic and periodontal pathogens has not been determined. the aim of this study was to identify the presence and quantities of representative cariogenic and periodontal pathogens in the oral cavi ...201425178742
nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms.chlorhexidine (chx) is a widely used antimicrobial agent in dentistry. herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure chx (nano-chx), and its mechanical profile and antimicrobial properties against oral biofilms.201425170958
proteomics of fusobacterium nucleatum within a model developing oral microbial community.fusobacterium nucleatum is a common oral organism that can provide adhesive and metabolic support to developing periodontal bacterial communities. it is within the context of these communities that disease occurs. we have previously reported whole cell proteomics analyses of porphyromonas gingivalis and streptococcus gordonii in early-stage communities with each other and with f. nucleatum, modeled using 18 h pellets. here, we report the adaptation of f. nucleatum to the same experimental condit ...201425155235
microbial signature profiles of periodontally healthy and diseased determine microbial profiles that discriminate periodontal health from different forms of periodontal diseases.201425139407
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