predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
identification of components in fusobacterium nucleatum chemostat-culture supernatants that are potent inhibitors of human gingival fibroblast proliferation.the present investigation concerned the effect of chemostat-culture cell-free supernatants of fusobacterium nucleatum on the growth and synthetic activity of human gingival fibroblasts in vitro. human gingival fibroblasts were cultured in fetal calf serum supplemented dulbecco-vogt medium containing various dilutions of conditioned or unconditioned bacterial culture medium. cell proliferation was monitored by assessing cell growth over 5 days or incorporation of [3h]-thymidine into dna. protein ...19911831499
multicenter evaluation of tetracycline fiber therapy. iii. microbiological a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ...19911832457
tetracycline fiber therapy monitored by dna probe and cultural methods.oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ...19911832458
use of synthetic oligonucleotide dna probes for the identification of different strains of fusobacterium nucleatum.the ability of three different oligonucleotide probes to identify different oral strains of fusobacterium nucleatum was tested. probes 119 and 214 were designed on the basis of known amino acid sequences from the n-terminal end of an outer membrane protein of f. nucleatum strain fev1. probe h 2.1, was a randomly cloned chromosomal dna fragment of 2.1 kbp. the specificities of the probes were examined by testing each of them against a panel of five other species of fusobacterium (six strains), or ...19911837056
neurons of the pretectal area convey spinal input to the motor thalamus of the cat.the aim of this study was to corroborate lesioning work (mackel and noda 1989), suggesting the pretectal area of the rostral midbrain acts as a relay between the spinal cord and the ventrolateral (vl) nucleus of the thalamus. for this purpose, extracellular recordings were made from neurons in the pretectal area which were antidromically activated by stimulation in the rostral thalamus, particularly in vl. the neurons were tested for input from the dorsal columns of the spinal cord, the dorsal c ...19911855554
prostaglandin production by amnion and decidual cells in response to bacterial from bacterial cultures have been tested for actions on prostaglandin biosynthesis by human amnion and decidual cells. the bacterial species, which are commonly associated with intrauterine infections, were group b streptococcus, escherichia coli, fusobacterium nucleatum, bacteroides fragilis, gardnerella vaginalis, neisseria gonorrhea, mycoplasma hominis and ureaplasma urealyticum. overall, low doses of bacterial products were stimulatory of amnion prostaglandin production, whereas high d ...19911857723
bacterial flora of the uterus of cows after calving on two hygienically contrasting farms.intrauterine swabs were obtained from cows after calving on two commercial dairy herds with contrasting hygienic environments and incidence of leucorrhea, and cultured aerobically and anaerobically. of 26 cows with a normal calving and puerperium, eight of 14 (57 per cent) were sterile on farm b where hygiene was poor, compared with five of 12 (42 per cent) on farm a where hygiene was better. two cows on farm b retained their placentas and subsequently developed metritis/endometritis. actinomyce ...19911858270
effects and aftereffects of ibogaine on morphine self-administration in rats.ibogaine, a naturally occurring alkaloid, has been claimed to be effective in treating addition to opiate and stimulant drugs. as a preclinical test of this claim, the present study sought to determine if ibogaine would reduce the intravenous self-administration of morphine in rats. ibogaine dose dependently (2.5-80 mg/kg) decreased morphine intake in the hour after ibogaine treatment (acute effect) and, to a lesser extent, a day later (aftereffect); while the acute effect could be attributed to ...19911868880
a study of the antigenic composition of the fragilis group of bacteroides.representative strains of 22 serotypes of the fragilis group of bacteroides and four non-fragilis control strains of b. melaninogenicus, b. disiens, b. bivius and fusobacterium nucleatum were tested by sds-page and immunoblotting with hyperimmune rabbit sera. sds-page showed 25 polypeptide bands but, after immunoblotting, 24 antigenic bands were observed in various combinations in all the strains. three of these were detected only in the control strains, whereas six others were present in differ ...19911875392
isolation, purification and characterisation of 2-oxoglutarate reductase from fusobacterium nucleatum.2-oxoglutarate reductase from fusobacterium nucleatum was isolated by thiol-disulphide interchange covalent chromatography. the enzyme was purified approximately 4000-fold and had a molecular mass of 68 kda. the michaelis constants for 2-oxoglutarate and nadh were 6.4 x 10(-5) and 0.4 x 10(-5), respectively. the involvement of sulphahydryl groups in catalysis was shown from the inhibition of 2-oxoglutarate reduction in the presence of 2,2'-dipyridyl disulphide and reactivation with 2-mercaptoeth ...19911884985
the use of genomic dna fingerprinting in studies of the epidemiology of bacteria in periodontitis.recent studies of microbial epidemiology emphasizing the genetic organization and distribution of organisms associated with orofacial infections have led to new insights into the possible origins of pathogenicity. studies into genetic heterogeneity, acquisition and transmission of these organisms have been markedly advanced by the utilization of the powerful technique of genomic dna fingerprinting. characteristic fingerprints for each bacterial isolate can be produced by cleavage of high molecul ...19911890219
dental bacterial plaques. nature and role in periodontal disease.antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ...19911890226
structure and bacterial receptor activity of a human salivary proline-rich glycoprotein.using an overlay technique, we previously showed that the gram-negative periodontal pathogen fusobacterium nucleatum binds to a glycoprotein of mr 89,000 (prakobphol, a., murray, p., and fischer, s.j. (1987) anal. biochem. 164, 5-11) in the parotid saliva of some individuals. we now show that deglycosylation of the purified glycoprotein results in loss of receptor activity. amino acid analysis of the protein core showed predominantly proline, glycine, and glutamic acid/glutamine, a characteristi ...19911894623
modeling bacterial damage to pulpal cells in vitro.there is increasing evidence that access to patent dentinal tubules by bacteria and their products rather than trauma from restorative materials is responsible for subsequent pulpitides. the purpose of this study was to compare the relative cytotoxicity of centrifugal fractions of two bacteria, fusobacterium nucleatum and treponema denticola, on l929 cells in monolayer cultures and in the "in vitro pulp chamber." neutrophilic chemotaxis assays and limmulus assays were performed to verify biologi ...19911895035
patterns of plasma sex hormone-binding globulin, thyroxine and thyroxine-binding globulin in relation to reproductive state and hibernation in female little brown bats.a sex hormone-binding globulin (shbg), which bound both oestradiol and dihydrotestosterone, was studied in the plasma of adult female little brown bats throughout the annual reproductive cycle. this protein was present at low baseline levels from september to may inclusive, months which correspond to the periods of hibernation, ovulation and early pregnancy. during the second half of pregnancy in june, shbg levels increased 15- to 30-fold and remained increased throughout lactation and anoestrus ...19911900322
identification, and susceptibility to seven antimicrobial agents, of 61 gram-negative anaerobic rods from periodontal pockets.sixty-one cultures of gram-negative anaerobic rods were isolated from deep periodontal pockets of patients with rapidly progressive periodontitis. isolates were speciated as bacteroides gingivalis (18 isolates), bacteroides intermedius (8), bacteroides oris (1), bacteroides gracilis (17) and fusobacterium nucleatum (17). their susceptibilities, to seven antimicrobial agents, were determined in vitro using a plate dilution technique. amoxycillin and amoxycillin with clavulanic acid were active ag ...19911901873
purification and characterization of 3-hydroxymethylglutaryl-coenzyme a reductase of schistosoma mansoni: regulation of parasite enzyme activity differs from mammalian host.the enzyme 3-hydroxymethylglutaryl-coa (hmg-coa) reductase plays a critical role in regulating the production of cholesterol, dolichols, and ubiquinones in mammals. the inhibition of this enzyme in schistosoma mansoni is accompanied by a cessation of egg production by the female parasite and a reduced ability of the parasite to properly glycoslyate their proteins. furthermore, we recently demonstrated that mevinolin, if given continuously over a period of 10-14 days, is a potent antischistosomal ...19911905241
compensatory increase in lactase expression by enterocytes of neonatal pigs on a low energy intake.a restricted energy intake in the immediate postnatal period has been found to result in a significant increase in the maximal expression of lactase by enterocytes from 2-week-old pigs. although villus size was significantly reduced on a low compared with a high food intake, total villus lactase activity was unaffected because of the compensatory increase in lactase expression by individual enterocytes.19911905556
biological functions of the water-insoluble fraction of mouse seminal vesicle fluid. i. suppression of the blastogenic response of lymphocytes.the effects of the water-insoluble fraction of mouse seminal vesicle fluid (wif-svf) on lymphocytes was investigated to clarify its role in reproductive immunity. wif-svf inhibited the blastogenic response of t-cells to concanavalin-a (con-a), but it did not inhibit the blastogenic response of b-cells to lipopolysaccharide (lps). pretreatment of splenocytes with wif-svf did not suppress the blastogenic response of splenocytes to con-a when treated cells were washed prior to culture. wif-svf did ...19911906049
leukemia inhibitory factor: does the cap fit? 19911906256
neuropeptide-y innervation of the rat spleen: another potential immunomodulatory neuropeptide.neuropeptide-y (npy) is a 36 amino acid peptide that acts as a chemical messenger in the central and peripheral nervous systems. npy often is found colocalized with the classical neurotransmitter norepinephrine (ne) and can potentiate the effects of this neurotransmitter postsynaptically in many systems. using immunocytochemistry for npy and specific lymphoid cell markers, we mapped the distribution of npy-positive nerve fibers in the rat spleen. npy-positive nerve fibers were present along the ...19911906353
protein-kinase-c-independent activation of arachidonate release and prostaglandin e2 formation in macrophages interacting with certain bacteria.certain bacterial species, of which we selected fusobacterium nucleatum, gardnerella vaginalis, peptostreptococcus anaerobius and propionibacterium acnes, were found to induce release of arachidonic acid in a dose- and time-dependent manner in mouse macrophages. the release of arachidonic acid showed a characteristic lag period of approximately 10 min and was accompanied by selective transformation into prostaglandin e2. bacteria killed by various methods caused a similar response, indicating th ...19911915341
isolation and purification of bacteriocin from prevotella intermedia (bacteroides intermedius).thirty-three out of 46 strains of prevotella intermedia (bacteroides intermedius) isolated from gingival deposits of patients with adult periodontitis possessed the ability to inhibit growth of p. intermedia strain atcc 25611, and 6 of the 33 showed strong inhibitory activity. thirteen isolates did not have the ability to inhibit the growth of p. intermedia strain atcc 25611. an inhibition factor from one of the isolates, p. intermedia th14 which had strong inhibition, was purified and character ...19911920011
control of enterococcus faecalis sex pheromone cad1 elaboration: effects of culture aeration and pad1 plasmid-encoded determinants.aeration of plasmid-free enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cad1 activity in culture filtrates. levels of two unrelated pheromones, cpd1 and cam373, were unaffected by culture aeration. aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pad1 trab mutations, verifying a link between trab function and pheromone "shutdown." tests with a series of pad1 mini-plasmids indicated that the ...19911924555
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
accumulation of plasma cells in inflamed sites: effects of antigen, nonspecific microbial activators, and chronic inflammation.plasma cells are common in chronically inflamed sites, including periodontal lesions. the aim of this study was to determine which factors contribute to this local accumulation of plasma cells. specifically, we sought to evaluate the effects of specific antigen and nonspecific activators from an infectious agent associated with chronic inflammation (fusobacterium nucleatum, an organism prominent in chronic periodontal lesions) and the effect of the chronic inflammation itself. chronic inflammati ...19911937760
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...19911940739
specificity of antibodies present in human periapical lesions.various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...19911940740
preliminary investigation of crystals of the neutral lipase from pseudomonas fluorescens.the neutral lipase from the bacteria pseudomonas fluorescens, marketed under the trade name lpl-200s, has been crystallized in a form suitable for x-ray diffraction analysis from 35% n-propanol at ph 8.5. the crystals are monoclinic prisms and are of space group c2 with a = 91.00 a, b = 47.17 a, c = 35.21 a and beta = 121.43 degrees. there is one molecule of the protein as the asymmetric unit of the crystals. the diffraction pattern extends to at least 1.6 a resolution and the crystals are extre ...19911942065
dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque.this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ...19911945492
[ecosystem of microbial flora in periodontal pockets, in vitro].this study was designed to analyze the succession of an ecosystem of microbial flora in periodontal pockets, in vitro. bacterial strains used in this study, were fusobacterium nucleatum atcc 10953, porphyromonas gingivalis 381, streptococcus sanguis atcc 10557 and streptococcus mutans omz 176. the bacterial cells, associated with beneficial, pathogenic or plaque forming activity, were cultured in brain heart infusion medium including hemin, vitamin-k1, l-cysteine hydrochloride and sodium thiogly ...19901966733
peptidoglycan precursor from fusobacterium nucleatum contains lanthionine.fusobacterium nucleatum was grown in the presence of [14c]udp. by means of sequential precipitation and chromatographic separation of the cytoplasmic content, a peptidoglycan [14c]udp pentapeptide containing lanthionine was isolated. this finding indicates that lanthionine is synthesized and incorporated as such during the assembly of the peptidoglycan.19911987169
microbiological and immunological aspects of experimental periodontal disease in rats: a review article.animal models in which microbiological and immunological aspects of periodontal disease can be studied prospectively seem well warranted. the rat bears much resemblance to man with respect to periodontal anatomy, development and composition of dental plaque, histopathology of periodontal lesions, and basic immunobiology. furthermore, reproducible methods are available for assessment of periodontal disease in rats, and detectable periodontal destruction can be induced in a few weeks in these anim ...19912002433
the helicobacter (campylobacter) pylori-colonized duodenal mucosa and gastric metaplasia.biopsies were obtained from non-ulcerated sites of the duodenum from 100 dyspeptic patients. helicobacter (campylobacter) pylori was cultivated from 19 of these biopsies. active chronic duodenitis (acd) was found in 17 biopsies and more than 5% gastric metaplasia in 20 biopsies. h. pylori as well as acd occurred with a significantly increased frequency when more than 5% gastric metaplasia was found in the duodenal biopsies. h. pylori on metaplastic tissue without acd was, however, seen in two ca ...19912018637
a randomized, placebo-controlled trial of doxycycline: effect on the microflora of recurrent periodontitis lesions in high risk patients.twenty-seven patients with a recent history of periodontal abscesses and/or loss of gingival attachment level (gal) despite active periodontal therapy were enrolled in a double-blind, randomized, placebo-controlled trial. clinical measurements and subgingival scaling were performed every 2 months. when a site exhibited greater than or equal to 2 mm loss of gal or a periodontal abscess, patients were administered either doxycycline at a dosage of 200 mg to start and 100 mg per day for 3 weeks, or ...19912027071
[right-sided endocarditis due to fusobacterium nucleatum].we present 1 case of right sided endocarditis caused by fusobacterium nucleatum in a patient with intravenous drug addiction and human immunodeficiency. the clinical features were fever, anemia, and pulmonary embolism. the echocardiogram showed a giant vegetation originated from the right atrial wall prolapsing in diastole into the right ventricle which disappeared after the patient presented pulmonary embolism. the clinical course was uncontrolled with empiric antimicrobial therapy but it was g ...19912047551
purification and characterization of a 65-kilodalton diisopropylfluorophosphate-binding protein in the outer membrane of fusobacterium nucleatum fev1.a 65-kilodalton protein was identified in the outer membrane of fusobacterium nucleatum fev1 by sds-page. the relative amount of the protein varied during growth, being greatest in stationary phase. the protein was radio-labeled by [125i]-lactoperoxidase treatment of live cells and was only partially extractable with 2% sodium dodecylsulfate (sds) at room temperature, and therefore assumed to be both exposed to the cell surface and peptidoglycan associated. in intact cells the protein bound diis ...19912047749
amoxycillin with clavulanic acid and tetracycline in periodontal therapy.the effects of tetracycline and amoxycillin with clavulanic acid on the clinical parameters and subgingival flora of eight patients with rapidly progressive periodontitis was assessed. subjects received either tetracycline 250 mg four times daily or amoxycillin 250 mg with clavulanic acid 125 mg three times daily for a period of 2 weeks together with subgingival scaling and root planning. both treatment regimens produced significant reductions in bleeding on probing and probing pocket depths whi ...19912050897
synergistic effect on blastogenesis in murine spleen cells of lipopolysaccharide, lipid a, and acid-degraded polysaccharide from fusobacterium nucleatum.interchangeable combinations of fusobacterium nucleatum fev1 lipopolysaccharide (lps) with its split products by acetic acid hydrolysis, i.e. lipid a (la) and degraded polysaccharide (ps), amplified the blastogenic response in murine spleen cell cultures as measured by [3h]thymidine uptake. athymic murine spleen cells precultured with lps-fev1 for 48 h (stage 1), washed twice and cultured together with fresh cells and either la or ps for 72 h (stage 2) gave a synergistic response over that found ...19912059480
amino acid utilization by fusobacterium nucleatum grown in a chemically defined medium.the present investigation evaluated amino acid utilization by 120 strains of fusobacterium nucleatum in a chemically defined medium and attempted to relate the patterns to 3 proposed subspecies of f. nucleatum. strains were inoculated into a chemically defined medium, with and without 2 g/l glucose, consisting of 14 inorganic salts, 21 amino acids, 23 vitamins and cofactors, and 7 purines and pyrimidines. after 7 days of anaerobic incubation, the spent culture medium, as well as the uninoculated ...19902080074
ingestion of bacteroides buccae, bacteroides oris, porphyromonas gingivalis, and fusobacterium nucleatum by human polymorphonuclear leukocytes in vitro.the phagocytic ingestion of clinical isolates and reference strains of bacteroides buccae, bacteroides oris, porphyromonas gingivalis and fusobacterium nucleatum by polymorphonuclear leukocytes (pmns) was studied. special attention was focused on the hydrophobicity of the strains. b. buccae strains, less or equally hydrophobic than pmns, were poorly ingested without opsonization. hydrophobic, but not hydrophilic, strains of b. oris and both hydrophilic and hydrophobic p. gingivalis and f. nuclea ...19902082243
the incidence of periodontopathic microorganisms in young children.thirty-six children in 4 cohorts of 0-1 week, 1-6 months, 1-2 years and 2-2 1/2 years of age were examined for the presence of black-pigmented bacteroides species and some other bacteria suspected of being involved in the subsequent development of periodontal destruction. none of the sought-after bacteria were detected in the first week of life. bacteroides intermedius and bacteroides melaninogenicus were detected as early as 1 month after birth. both of these bacteria were detected in 16-37% of ...19902087345
role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces.progress in characterizing the receptors that promote bacterial attachment to teeth and oral epithelial cells has suggested that hidden molecular segments may frequently be involved. such cryptic receptors, referred to as 'cryptitopes', may become exposed by several mechanisms. hidden segments of salivary acidic proline-rich proteins evidently become exposed when the molecules undergo a conformational change as they adsorb to apatitic mineral. adhesins of actinomyces viscosus and certain other p ...19902088213
clinical and microbial evaluation of treatment regimens to reduce denture stomatitis.this study evaluated the effect of an antimicrobial mouthrinse, denture soft relines, and a placebo rinse on the clinical findings and microbial flora of 78 patients with denture stomatitis. for 28 days the study and control groups performed oral rinses and denture soaks. reline group patients had maxillary denture soft relines that were changed every 7 days. at the end of the study, the mean severity of denture stomatitis was significantly less in the groups that used the study rinse (p less th ...19902088373
serological investigation of refractory human adult periodontitis.this study examined the difference between the serum antibody profiles in refractory adult periodontitis patients (group a), and compared to those (group b) who responded well to conventional periodontal treatment. the levels of specific igg antibody to actinobacillus actinomycetemcomitans, bacteroides gingivalis, fusibacteriumnucleatum, and eikenella corrodens were assessed in a group of 19 patients (group a) and 11 patients (group b). specific igg serum antibody levels were estimated using bio ...19902096960
distribution and frequency of fusobacterium nucleatum subspecies in the human oral cavity.three reference strains of fusobacterium nucleatum and 32 human oral isolates were compared by a variety of physiological tests, enzyme electrophoretic profiles, sds-page patterns, dna base composition and hybridization to test their possible site specificity and frequency of the recently described subspecies of f. nucleatum. nine of the 11 isolates assigned to f. nucleatum subspecies nucleatum were from diseased sites, whereas isolates from healthy sites were all identified as f. nucleatum subs ...19902098710
sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria.a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ...19902108991
serum antibodies to periodontal bacteria.the purpose of this study was to determine how serum antibodies reactive with periodontitis-associated bacteria with relates to the diagnosis of periodontitis subjects. study groups included localized juvenile periodontitis (ljp) subjects, severe periodontitis (sp) subjects, chronic adult periodontitis (ap) subjects, and age matched controls. twenty-two bacterial strains, representing 18 different species most commonly found in early onset periodontitis were evaluated using serum from ljp, sp, a ...19902117654
studies of the subgingival microflora in patients with acquired immunodeficiency syndrome.two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ...19902123926
monoclonal antibody-coated latex agglutination assay for identification of actinobacillus actinomycetemcomitans.three monoclonal antibodies (mabs) to lipopolysaccharide of actinobacillus actinomycetemcomitans strain y4 (serotype b) and eight mabs to a serotype b-specific polysaccharide antigen of strain y4 were obtained. latex particles sensitized with an mab to the y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of a. actinomycetemcomitans, but not with haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, porphyromonas (bacteroides) ...19902124492
a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ...19902131168
binding specificity of fusobacterium nucleatum to human erythrocytes, polymorphonuclear leukocytes, fibroblasts, and hela cells.eighteen strains of fusobacterium nucleatum were studied with respect to their adherence to human erythrocytes, polymorphonuclear leukocytes, fibroblasts, and hela cells. four types of adherence were found: 1) sugar-sensitive adherence 2) amino acid-sensitive adherence 3) unknown adherence 4) weak adherence. there was no obvious correlation between the patterns of hemagglutination and adherence to other cells. the results indicate not only a complex network of bacterial receptors on human cells, ...19902141873
interaction of fusobacterium nucleatum 191 with human peripheral blood lymphocytes.fusobacterium nucleatum is frequently isolated in high numbers from the subgingival plaque of patients with periodontal diseases. adherence of these bacteria to several host tissues is lectin-like in that it is inhibited by galactose, lactose, and n-acetyl-galactosamine. since f. nucleatum has the capacity to activate human peripheral blood lymphocytes, the purpose of this study was to determine whether f. nucleatum adheres to lymphocytes and, if so, to determine whether lectin-like interactions ...19902148949
identification and characterization of human endothelial cell membrane binding sites for tissue plasminogen activator and urokinase.cultured human endothelial cells synthesize and secrete two types of plasminogen activator, tissue plasminogen activator (t-pa) and urokinase (u-pa). previous work from this laboratory (hajjar, k.a., hamel, n. m., harpel, p. c., and nachman, r. l. (1987) j. clin. invest. 80, 1712-1719) has demonstrated dose-dependent, saturable, and high affinity binding of t-pa to two sites associated with cultural endothelial cell monolayers. we now report that an isolated plasma membrane-enriched endothelial ...19902154465
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
regulation of human monocyte/macrophage function by extracellular matrix. adherence of monocytes to collagen matrices enhances phagocytosis of opsonized bacteria by activation of complement receptors and enhancement of fc receptor inflammation monocytes emigrate from the peripheral circulation into an extravascular area rich in extracellular matrix proteins. in this milieu, phagocytes ingest and kill invading pathogens. in the present studies, we found that monocytes adhered to type i collagen gels phagocytized 2.5-12-fold more opsonized escherichia coli, staphylococcus aureus, streptococcus pyogenes, and streptococcus pneumoniae than plastic-adherent monocytes. the rate of phagocytosis and the number of bacteria inges ...19902168442
detection of bovine viral diarrhea virus in serum from cattle by dot blot hybridization assay.a dot blot hybridization assay was developed for use as a rapid screening test to detect bovine viral diarrhea virus (bvdv) in serum from infected cattle. a 1.1. kilobase cdna, prepared from the bvdv genome, was molecularly cloned and used in this study. insert cdna was removed from the puc9 plasmid vector by pst-i restriction endonuclease digestion and purified from plasmid dna by agarose electrophoresis and electroelution. the hybridization probe was prepared by nick translation in the presenc ...19902175527
transferable tet m in fusobacterium nucleatum.two tetracycline-resistant fusobacterium nucleatum strains were able to transfer, by conjugation, tetracycline resistance and the tet m determinant to recipient f. nucleatum, peptostreptococcus anaerobius, and enterococcus faecalis strains at a frequency of 10(-1) to 10(-8).19902178339
renin is sorted to the regulated secretory pathway in transfected pc12 cells by a mechanism which does not require expression of the pro-peptide.the rat pheochromocytoma cell line pc12 targets secretory proteins into two distinct pathways. when dna encoding human prorenin was transfected into pc12 cells, the protein was sorted into the regulated secretory pathway and released with similar kinetics to noradrenaline upon carbachol stimulation. to determine whether information for targeting prorenin lies within the pro-peptide we have transfected pc12 cells with a construct lacking the pro-peptide coding sequence. the transformed line secre ...19902194793
susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations.a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ...19902202809
the rna polymerases of porphyromonas gingivalis and fusobacterium nucleatum are unrelated to the rna polymerase of escherichia coli.western blot analysis that used antisera to the e. coli core enzyme and sigma factors was used for examination of the rna polymerase of actinobacillus actinomycetemcomitans, streptococcus mutans, fusobacterium nucleatum, and porphyromonas gingivalis. both antisera reacted with proteins in a. actinomycetemcomitans and s. mutans whole-cell extracts. reactions were seen with some f. nucleatum proteins when the anti-core rna polymerase antisera were used, but the cross-reacting proteins were not of ...19902204641
cloning of wild-type pseudomonas solanacearum phca, a gene that when mutated alters expression of multiple traits that contribute to virulence.pseudomonas solanacearum undergoes a spontaneous mutation that pleiotropically reduces extracellular polysaccharide (eps) production, endoglucanase activity, and virulence and increases motility. we refer to the process that coordinately affects these traits as phenotype conversion (pc) and the resulting mutants as pc types. previous research with the wild-type strain aw1 suggested that inactivation of a single locus could mimic phenotype conversion (t. p. denny, f. w. makini, and s. m. brumbley ...19902211505
regulation of fructose metabolism and polymer synthesis by fusobacterium nucleatum atcc for the anaerobic growth of fusobacterium nucleatum atcc 10953 can be derived from the fermentation of sugar (fructose) or amino acid (glutamate). during growth on fructose, the cells formed large intracellular granules which after extraction yielded glucose by either acid or enzymatic hydrolysis. the endogenous polymer was subsequently metabolized, and after overnight incubation of the cells in buffer, the glucan granules were no longer detectable by electron microscopy. anaerobically, w ...19902211506
plaque and chronic inflammatory periodontal disease. a question of ecology.the nature of the relationship between dental plaque and chronic inflammatory periodontal disease (cipd) remains unclear, although there is no doubt that plaque is the direct cause. non-specific, specific and exogenous hypotheses have been proposed to explain plaque-host relationships. current evidence indicates that plaque is part of the natural human microflora, one of many such in nature, and that disruption of oral microbial ecology, due primarily to diet texture changes, leads to gingivitis ...19902212083
recolonization of the subgingival microflora after scaling and root planing in human periodontitis.the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ...19902213468
glucose homeostasis in the rat after liver transplantation. 19902219327
radioimmunotargeting of human tumour cells in immunocompetent animals.a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ...19902223574
radioimmunotargeting of human tumour cells in immunocompetent animals.a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ...19902223574
proposal of three subspecies of fusobacterium nucleatum knorr 1922: fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov.heterogeneity among isolates of fusobacterium nucleatum has been recognized for many years. the phenotypic properties of 340 strains considered to be f. nucleatum were examined. while these strains were phenotypically similar and fit the description of f. nucleatum, they could be differentiated into three groups on the basis of electrophoretic patterns of whole-cell proteins and dna homology. strains in groups i and ii showed greater than 80% dna homology within groups and less than 75% similari ...19902223601
stimulation of serotonin2 receptors in the ventrolateral medulla of the cat results in nonuniform increases in sympathetic outflow.topical application of the serotonin2 agonist, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane or doi, in a dose of 30 micrograms/side to the intermediate area of the ventrolateral surface of the medulla produced a significant increase in mean arterial pressure with no significant change in heart rate both in intact animals (n = 8) and in cervically vagotomized animals (n = 3). the pressor response of doi was blocked by pretreatment of the intermediate area with ketanserin, a serotonin2 antagonist ...19902225358
[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...19902238764
the biotin-dependent sodium ion pump glutaconyl-coa decarboxylase from fusobacterium nucleatum (subsp. nucleatum). comparison with the glutaconyl-coa decarboxylases from gram-positive bacteria.membrane preparations of fusobacterium nucleatum grown on glutamate contain glutaconyl-coa decarboxylase at a high specific activity (13.8 nkat/mg protein). the enzyme was solubilized with 2% triton x-100 in 0.5 m nacl and purified 63-fold to a specific activity of 870 nkat/mg by affinity chromatography on monomeric avidin-sepharose. the activity of the decarboxylase was strictly dependent on na+ (km = 3 mm) and was stimulated up to 3-fold by phospholipids. the glutaconyl-coa decarboxylases from ...19902244788
[bcl-2 gene involved in t(14;18) translocation]. 19902255071
specific amplification of rearranged immunoglobulin variable region genes from mouse hybridoma this article we show how the polymerase chain reaction (pcr) and primers designed for conserved sequences of leader (l), framework one (fr1) and constant (const) regions of immunoglobulin light and heavy chain genes can be used for the cloning and sequencing of rearranged antibody variable regions from mouse hybridoma cells. rna was extracted from the mouse hybridoma cells secreting mabs: ior-t3a (anti-cd3), c6 (anti-p1 of n. meningitidis b385), ior-t1 (anti-cd6), cb-cea.1 (anti-carcinoembryo ...19902258182
lysocardiolipin formation and reacylation in isolated rat liver mitochondria.liver mitochondrial cardiolipin (cl) is distinguished from other phospholipids by the presence of linoleoyl in almost all molecular species, and the biosynthesis of these species is not yet understood. the present study was carried out in order to test the hypothesis that the linoleoyl proportion of cl may be specifically enriched by a deacylation-reacylation cycle. incorporation of [14c]glycerol 3-phosphate into the metabolites of the cl pathway was accompanied by formation of 14c-labelled mono ...19902268287
coaggregation of oral candida isolates with bacteria from bone marrow transplant vitro coaggregation between candida species isolated from immunosuppressed bone marrow transplant recipients and oral bacteria was investigated. each candida strain showed a different pattern of coaggregation with the 22 bacterial strains studied. two strains of lactobacillus amylovorus isolated from separate bone marrow transplant patients and fusobacterium nucleatum (vpi 10197) coaggregated with all candida strains. ten bacterial strains showed no coaggregation with the candida strains. a v ...19902279992
associations between six dna probe-detected periodontal bacteria and alveolar bone loss and other clinical signs of periodontitis.the purpose of the present study was to assess the associations between the presence and amounts of actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius, eikenella corrodens, wolinella recta, and fusobacterium nucleatum in the periodontal pocket and the degree of alveolar bone loss and other clinical signs of periodonitis, such as probing pocket depth, attachment level, and presence of bleeding on probing at the same site. the study material comprised 16 subjects with or ...19902288212
microbial patterns in pooled subgingival plaque samples from young adults with advanced marginal periodontitis.different bacterial profiles of subgingival plaque have been described for different clinical categories of marginal periodontitis. in the present investigation the subgingival microflora was studied in young adults with advanced marginal periodontitis. from 12 patients pooled subgingival plaque samples from 5 advanced stages of diseased sites were examined by direct differential phase-contrast microscopy and by cultivation on enriched and selective media. the proportions of the following genera ...19902293348
capnophilic and anaerobic bacteremia in neutropenic patients: an oral source.the currently accepted empiric antibiotic therapy for bacterial infections in neutropenic patients may not cover the possibility of capnophilic and anaerobic bacteremia. many of these infections develop in patients with severe mucositis or periodontitis, and the type of organisms recovered also suggests an oral source of infection. we present two cases of bacteremia in neutropenic patients who had been empirically treated with ceftazidime and piperacillin plus amikacin. in the first case a beta- ...19902305182
species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria.oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ...19902312676
growth stimulation of treponema denticola by periodontal microorganisms.previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ...19902321929
diisopropylfluorophosphate-binding proteins in the outer membrane of fusobacterium nucleatum: strain variations.six strains of fusobacterium nucleatum were tested for their ability to react with [3h]diisopropylfluorophosphate (dfp), a serine protease inhibitor. several cytoplasmic proteins were labelled but the strongest labelling was regularly observed in a few outer membrane proteins. the number and the molecular mass of the proteins detected varied according to the strain tested. a 61 kda protein was labelled in all strains tested, including the two type strains atcc 10953 and atcc 25586. a 65 kda prot ...19902323539
long-term clearance of inhaled uo2 particles from the pulmonary region of the rat.this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ...19902323929
susceptibility and resistance of plaque bacteria to minocycline.the in vitro susceptibility of 55 strains of subgingival plaque bacteria to minocycline was determined. a concentration of 1 microgram/ml minocycline was found to inhibit 85% of the strains tested and the mic ranged from 0.03 to 32 micrograms/ml. for 71% of the strains tested the mbc was at least 4 times greater than the corresponding mic, suggesting a bacteriostatic activity for minocycline. a concentration of 20 mg/ml of magnesium ions was capable of neutralizing 8 micrograms/ml of minocycline ...19902324922
genetic basis of tetracycline resistance in urogenital bacteria.the distributions of the nucleotide sequences related to the tetracycline resistance determinants tet k, tet l, tet m, and tet o were studied by dot blot hybridization with randomly chosen clinical urogenital tract isolates of viridans group streptococci, streptococcus agalactiae, enterococcus faecalis, gardnerella vaginalis, lactobacillus spp., fusobacterium nucleatum, peptostreptococcus spp., and veillonella parvula. among the peptostreptococcus spp., 79% of the isolates hybridized with one (6 ...19902327774
inhibitory effects of neuropeptide y on adenylate cyclase of rabbit ciliary processes.the inhibitory effect of neuropeptide y (npy) was studied on the adenylate cyclase (ac) activity in homogenates of rabbit ciliary processes and compared with that of the alpha 2-adrenergic agonist clonidine (cln). npy inhibited basal ac activity as well as ac activity stimulated by isoproterenol (iso), vasoactive intestinal polypeptide (vip) or forskolin (fsk). the extent of this inhibition corresponded well to the inhibition elicited by cln. the inhibitory effects of npy and cln appeared to be ...19902335109
multiple periodontal abscesses after systemic antibiotic therapy.multiple periodontal abscesses were reported in medically compromised patients. we examined patients with a non-contributory medical history referred for the treatment of numerous periodontal abscesses. all patients had taken oral broad spectrum antibiotics 1 to 3 weeks prior to the outburst of the abscesses (8 patients: penicillin, 2 patients: tetracycline). the patients suffered from advanced periodontal disease, 82% of the examined sites showed probing depths greater than 3 mm, 56% attachment ...19902347952
growth conditions and outer membrane proteins of fusobacterium nucleatum.fusobacterium nucleatum strains atcc 10953, atcc 25586, f1 f3, f6, and fev1 were grown in different media. the influence of growth conditions on the outer membrane proteins (omps) was analyzed by sodium dodecyl sulfate--polyacrylamide gel electrophoresis (sds-page). there were no apparent differences in the outer membrane protein profiles when cells in the same phase of growth in various rich media were compared. differences, however, were observed between early logarithmic phase and stationary ...19902349450
blastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells stimulated with lipopolysaccharide, lipid a and acid-degraded polysaccharide from fusobacterium nucleatum.lipopolysaccharide of fusobacterium nucleatum strain fevl was split by acid hydrolysis. the split products, i.e. lipid a and degraded polysaccharide were mitogenic for murine spleen cells as measured by uptake of [3h]thymidine. the uptake of [3h]thymidine was dose-dependent. incubation of spleen cells with stimulants for 3 days resulted in a polyclonal activation of immunoglobulin synthesis. higher mitogenic response and immunoglobulin production were found in spleen cells of athymic mice compar ...19902369526
risk factors for kaposi's sarcoma in the vancouver lymphadenopathy-aids our ongoing cohort study of homosexual men, the ratio of new kaposi's sarcoma (ks) cases to new opportunistic infections (oi) during the periods 1982-1985, 1986-1987, and 1988-1989 fell from 0.75 (9 ks: 12 oi) to 0.57 (12 ks:21 oi) to 0.27 (4 ks:15 oi), respectively. to examine factors associated with the development of ks as compared to oi, we compared antecedent risk factors in 25 ks cases and 48 oi "controls." in univariate analyses, several classical hiv risk factors including numbers of ...19902395081
resistance to beta-lactam antibiotics in anaerobic bacteria.the known mechanisms of beta-lactam resistance in anaerobic bacteria involve production of beta-lactamases, alteration of penicillin-binding proteins, and blocked penetration of beta-lactam antibiotics through bacterial outer membranes. the most important factor in beta-lactam resistance is production of beta-lactamases, which have been found in various bacteroides, fusobacterium, and clostridium species. beta-lactam resistance in bacteroides fragilis is commonly mediated by beta-lactamases that ...19902406875
modulation of hla-dr antigens in the gingival epithelium in vitro by heat-killed fusobacterium nucleatum and e. coli lipopolysaccharide.the in vitro influence of the periodontopathic organism fusobacterium nucleatum (fn) on gingival tissue was examined using an organ culture system. treatment of gingival explants obtained from periodontally diseased sites with suspensions of fn, stimulated the expression of hla-dr antigens by langerhans cells (lc) in a dose-dependent fashion, and produced a maintenance of the lc markers t6 and atpase. similar effects were seen when e. coli lipopolysaccharide (lps) was substituted for suspensions ...19852414424
specificity of monoclonal antibodies reactive with fusobacterium nucleatum: effect of formalin fixation.monoclonal antibody specific for fusobacterium nucleatum was reacted with untreated and formalin fixed f. nucleatum cells by an enzyme-linked immunosorbent assay (elisa) and by indirect immunofluorescence. treatment of bacterial cells with formalin destroyed the antigenic determinant responsible for reactivity with this monoclonal antibody in both assays. formalin fixation had no effect on hemagglutination activity (ha) of f. nucleatum cells or reactivity with polyvalent rabbit antiserum in doub ...19852415637
target size of ca-pumps in pig coronary artery smooth muscle.the target sizes of the oxalate-independent ca uptake by the plasma membrane enriched fraction f2, and the oxalate-stimulated ca uptake by a fraction f3 slightly enriched in the endoplasmic reticulum were determined by radiation inactivation. the oxalate-independent ca uptake was inactivated with a d37 value of 1.96 +/- 0.30 mrad but the oxalate-stimulated ca uptake had a d37 value of 0.45 +/- 0.07 mrad. thus, in the smooth muscle the oxalate-stimulated ca uptake appeared to be due to a structur ...19852415797
[malaria prevention. chemotherapy and immune prophylaxis. 3. immune response of infected organism. perspectives of malaria prevention]. 19862423092
humoral antibody response against bacteroides gingivalis-specific antigen recognized by monoclonal antibody in adult periodontal antigen recognized by monoclonal antibody specific for bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. the purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and silver staining, and the pattern of sds-page agreed with that of immunoblotting. the molecule exhibited an apparent molecular weight of about 62,000 by sds-page. the antigen was sensitive ...19862426200
differential expression of class ii (dr & dq) antigens by human gingival langerhans' cells and keratinocytes in vitro.the expression of the class ii products dr and dq on human gingival epithelium was examined using immunofluorescence and immunoperoxidase staining. differential expression of class ii antigens was seen in chronic gingivitis in adults, with t6(+) dr(+) cells being more numerous than t6(+) dq(+) cells. the periodontopathic organism fusobacterium nucleatum (fn) induced dq expression on langerhans' cells (lc) during in vitro explant culture of gingival tissue. this effect was mimicked by endotoxin ( ...19872435875
possible existence of a novel amphipathic immunostimulator in the phenol-water extracts of mycobacteriaceae.the extracts having diverse immunostimulating activities were obtained as a water-phase fraction from four bacterial species representing the 4 genera (mycobacterium, nocardia, gordona, and rhodococcus) of mycobacteriaceae by the phenol-water method, which is commonly used for extraction of endotoxic lipopolysaccharides (lps) from gram-negative bacteria and amphipathic substances from gram-positives. these fractions, especially those of g. aurantiaca and r. terrae, showed strong stimulatory effe ...19872441233
conformations of intermediates in the folding of the pancreatic trypsin inhibitor.intermediates in the folding pathway of the bovine pancreatic trypsin inhibitor (pti) have been examined by 1h nuclear magnetic resonance (n.m.r.). the intermediates were trapped during the reoxidation and consequent refolding of reduced pti by alkylating free thiols; each intermediate contained different disulphide linkages. the n.m.r. spectra reveal that conformational features of the native protein are present in the intermediate containing just one of the three normal disulphide linkages (30 ...19872443711
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