PMID(sorted descending)
in vitro evaluation of planktonic growth on experimental cement-retained titanium surfaces.background the purpose of this study was to compare the effects of selected cements, or their combination with titanium, on the growth of two periodontopathic bacteria: prevotella intermedia (pi) and fusobacterium nucleatum (fn). material and methods this study was comprised of several experimental groups: 1) dental luting cements (glass ionomer cement, methacrylate-based resin cement, zinc-oxide eugenol cement, eugenol-free zinc oxide cement; 2) titanium discs; and 3) titanium combination cemen ...201627058704
comparison of fusobacterium nucleatum and porphyromonas gingivalis lipopolysaccharides clinically isolated from root canal infection in the induction of pro-inflammatory cytokines secretion.the aim of this study was to compare the biological activity of lipopolysaccharides (lps) purified from fusobacterium nucleatum and porphyromonas gingivalis strains, both isolated from primary endodontic infection (pei) in the levels of il-1β and tnf-α released by macrophage cells. moreover, lps was purified from f. nucleatum and p. gingivalis american type collection (atcc) and its biological activity was compared to respectively clinical isolates strains. f. nucleatum and p. gingivalis strains ...201727058385
microbiota-based model improves the sensitivity of fecal immunochemical test for detecting colonic lesions.colorectal cancer (crc) is the second leading cause of death among cancers in the united states. although individuals diagnosed early have a greater than 90% chance of survival, more than one-third of individuals do not adhere to screening recommendations partly because the standard diagnostics, colonoscopy and sigmoidoscopy, are expensive and invasive. thus, there is a great need to improve the sensitivity of non-invasive tests to detect early stage cancers and adenomas. numerous studies have i ...201627056827
high-throughput dental biofilm growth analysis for multiparametric microenvironmental biochemical conditions using biofilm formation is not only a precursor to tooth decay, but also induces more serious systematic health problems such as cardiovascular disease and diabetes. understanding the conditions promoting colonization and subsequent biofilm development involving complex bacteria coaggregation is particularly important. in this paper, we report a high-throughput microfluidic 'artificial teeth' device offering controls of multiple microenvironmental factors (e.g. nutrients, growth factors, dissol ...201627045372
a dual-species microbial model for studying the dynamics between oral streptococci and periodontal pathogens during biofilm development on titanium surfaces by flow cytometry.the association of the pioneer organisms streptococcus mutans atcc 25175 or streptococcus oralis atcc 9811 with secondary colonizers fusobacterium nucleatum atcc 25586 or porphyromonas gingivalis atcc 33277 during biofilm development on titanium surfaces was evaluated by flow cytometry (fcm) using specific polyclonal antibodies. elisa and fcm were employed, revealing high antibody sensitivity and specificity. biofilm formation of four dual-species combinations was analyzed by crystal violet stai ...201627032997
accuracy of commercial kits and published primer pairs for the detection of periodontopathogens.despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. the aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens.201627020914
assessment of periodontopathogens in subgingival biofilm of banded and bonded molars in early phase of fixed orthodontic assess the prevalence and occurrence of eleven periodontopathogens in subgingival biofilm of banded and bonded molars during the first period of fixed orthodontic treatment. subjects were selected from patients referred to orthodontic treatment and were divided in two groups: group a comprised fifteen patients (14.4±2.45 years of age) who received orthodontic bands on first permanent molars and group b of ten patients (15.7±1.87 years of age) with directly bonded tubes on the labial surface o ...201627020873
antibacterial effect of silver (i) carbohydrate complexes on oral pathogenic key species in was the aim of this study to evaluate the antibacterial impact of two silver(i) carbohydrate complexes with tripodal thioglycosides, namely tris[2-(β-d-thio-glucopyranosyl)ethyl]-amine-silver(i)-nitrate (3) and tris[2-(α-d-thio-manno-pyranosyl)ethyl]-amine-silver(i)-nitrate (4), on five oral pathogenic bacterial strains. furthermore, cytocompatibility was tested using human gingival fibroblasts (hgf).201627009305
association of fusobacterium nucleatum infection with colorectal cancer in chinese investigate fusobacterium nucleatum (f. nucleatum) abundance in colorectal cancer (crc) tissues and its association with crc invasiveness in chinese patients.201627004000
influence of different instrumentation modalities on the surface characteristics and biofilm formation on dental implant neck, in evaluate surface characteristics of implants after using different instruments and biofilm formation following instrumentation.201727000771
oral microbiota species in acute apical endodontic abscesses.acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions.201626983837
intracanal antibiotic medication for sustained root surface disinfection-a laboratory measure the release of an antibiotic mixture of ciprofloxacin, cerfuroxim and metronidazole (trevitamix, tvm) through human dentine and to assess the growth inhibition of fusobacterium nucleatum.201526966464
effect of alendronate on the progression of periodontitis induced by porphyromonas gingivalis and fusobacterium nucleatum: a study in rats.the study aimed to investigate the effect of alendronate (aln) on the inhibition of alveolar bone loss in experimental periodontitis in wistar rats.201626955836
comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques.the prevalence and amounts of periodontal pathogens detected in bacteraemia samples after tooth brushing-induced by means of four diagnostic technique, three based on culture and one in a molecular-based technique, have been compared in this study.201626946197
[how some commensal bacteria would exacerbate colorectal carcinogenesis?].the gut microbiota maintains a relationship with its host with strong mutual benefits. changes in the composition of the intestinal microbiota have been detected in colorectal cancer patients to the extent that it is now considered as a real contributing factor in this pathology. in this review, we focus on three commensal bacterial species, namely bacteroides fragilis, fusobacterium nucleatum, and escherichia coli, which seem to emerge as pathogens and to contribute to colorectal carcinogenesis ...201626936175
influence of a triclosan toothpaste on periodontopathic bacteria and periodontitis progression in cardiovascular patients: a randomized controlled trial.triclosan/copolymer toothpaste is effective in controlling plaque and gingivitis and in slowing the progression of periodontitis. this study describes its influence on microbiological and clinical outcomes, over a 5-year period, in patients with established cardiovascular disease (cvd).201726932733
fad-i, a fusobacterium nucleatum cell wall-associated diacylated lipoprotein that mediates human beta defensin 2 induction through toll-like receptor-1/2 (tlr-1/2) and tlr-2/6.we previously identified a cell wall-associated protein from fusobacterium nucleatum, a gram-negative bacterium of the oral cavity, that induces human beta defensin 2 (hbd-2) in primary human oral epithelial cells (hoecs) and designated it fad-i (fusobacterium-associated defensin inducer). here, we report differential induction of hbd-2 by different strains of f. nucleatum; atcc 25586 and atcc 23726 induce significantly more hbd-2 mrna than atcc 10953. heterologous expression of plasmid-borne fa ...201626930710
[association between oral conditions and colorectal cancer: a literature review and synthesis].chronic periodontitis and tooth loss are chronic oral conditions that, in recent decades, have been implicated in the occurrence of certain types of cancer. in this review, we address the question of whether colorectal cancer is associated with these oral conditions.201626923863
morphological and functional adaptations of fusobacterium nucleatum exposed to human neutrophil peptide-1.we recently demonstrated that fusobacterium nucleatum can resist to human neutrophil peptide (hnp)-1 by decreasing its membrane permeability and increasing its proliferation and biofilm formation. in this continuation study, we aimed to further evaluate and explain these resistance properties by determining the morphological and functional adaptations of f. nucleatum, using transmission electron microscopy (tem).201626923748
effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic investigate the effect of the roselle calyx extract (rce) (hibiscus sabdariffa l.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria.201626919939
early colonization of the oral cavity in 6- and 12-month-old infants by cariogenic and periodontal pathogens: a case-control study.the colonization of the oral cavity by cariogenic and periodontal pathogens occurs earlier than previously thought. this study aimed to identify the presence and quantity of representative cariogenic and periodontal pathogens in the oral cavities of 6- and 12-month olds and to evaluate the influence of c-section delivery on early streptococcus mutans (sm) colonization of the oral cavity. the research cohort was composed of 59 infants (35 infants were delivered vaginally and 24 via c-section) and ...201626914065
effects of repeated screw tightening on implant abutment interfaces in terms of bacterial and yeast leakage in vitro: one-time abutment versus the multiscrewing technique.screw loosening can damage the interfaces of implant components, resulting in susceptibility to contamination of the internal parts by microorganisms. the aim of this study was to investigate the impact of abutment screw retightening on the leakage of two different types of bacteria, streptococcus sanguinis and fusobacterium nucleatum, and of the yeast candida albicans. two types of implant-abutment systems with tube-in-tube interfaces were tested. groups a and b each used a different type of sy ...201626901305
registered report: fusobacterium nucleatum infection is prevalent in human colorectal carcinoma.the reproducibility project: cancer biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. the papers, which were published between 2010 and 2012, were selected on the basis of citations and altmetric scores (errington et al., 2014). this registered report describes the proposed replication plan of key experiments from 'fusobacterium nucleatum in ...201626882501
microbiological distribution of six periodontal pathogens between untreated italian and dutch periodontal compare prevalence and microbial load of six periodontal pathogens between italian and dutch patients affected by chronic periodontitis, using oligonucleotide probe technology.201626870849
isph-rps1 and isph-ubia: "rosetta stone" proteins.the protein isph, (e)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate (hmppp) reductase, is an essential 4fe-4s cluster-containing protein in the methylerythritol phosphate pathway for isoprenoid biosynthesis. using a sequence similarity network we found that there are >400 isph proteins that are about twice as large as most of the isphs studied to date since their isph domains are fused to either the ribosomal protein s1 (rps1), or to a ubia (4-hydroxybenzoate octaprenyltransferase)-like protein. m ...201526865948
symptoms of periodontitis and antibody responses to porphyromonas gingivalis in juvenile idiopathic arthritis.the association between rheumatoid arthritis (ra) and periodontitis is well established. some children with juvenile idiopathic arthritis (jia) phenotypically resemble adults with ra, characterized by the presence of anti-cyclic citrullinated peptide (ccp) antibodies. we sought to investigate an association between ccp-positive jia and symptoms of periodontitis and antibodies to oral microbiota.201626861944
case series of patients with fusobacterium nucleatum bacteremia with emphasis on the presence of cancer.fusobacterium nucleatum is anaerobic oral microbiota that might be associated with cancer. we reported 22 consecutive cases of patients (mean age of 63.8 years (range 34-89), 59.1% male) with f. nucleatum bacteremia that were admitted to a university hospital over a 10-year period. in 17 (77.2%) of these patients, f. nucleatum was the sole possible pathogen. seven of the 22 patients (31.8%) had active cancer: esophagus carcinoma (n = 3), hematologic malignancies (n = 1), gastrointestinal stromal ...201626853831
periodontitis induced by porphyromonas gingivalis drives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune identify a causal mechanism responsible for the enhancement of insulin resistance and hyperglycaemia following periodontitis in mice fed a fat-enriched diet.201726838600
association of fusobacterium nucleatum with immunity and molecular alterations in colorectal cancer.the human intestinal microbiome plays a major role in human health and diseases, including colorectal cancer. colorectal carcinogenesis represents a heterogeneous process with a differing set of somatic molecular alterations, influenced by diet, environmental and microbial exposures, and host immunity. fusobacterium species are part of the human oral and intestinal microbiota. metagenomic analyses have shown an enrichment of fusobacterium nucleatum (f. nucleatum) in colorectal carcinoma tissue. ...201626811607
fusobacterium nucleatum infections: clinical spectrum and bacteriological features of 78 cases.few series describe the clinical spectrum of fusobacterium spp. infections. among them, fewer discuss f. nucleatum, even though there are many clinical cases.201626783023
total antioxidant capacity and total oxidant status in saliva of periodontitis patients in relation to bacterial load.the detection of salivary biomarkers has a potential application in early diagnosis and monitoring of periodontal inflammation. however, searching sensitive salivary biomarkers for periodontitis is still ongoing. oxidative stress is supposed to play an important role in periodontitis progression and tissue destruction. in this cross-sectional study, we investigated total antioxidant capacity (tac) and total oxidant status (tos) in saliva of periodontitis patients compared to healthy controls and ...201526779448
selective responses of human gingival fibroblasts and bacteria on carbon fiber reinforced polyetheretherketone with multilevel nanostructured tio2.the long-term success of dental implants relies not only on stable osseointegration but also on the integration of implant surfaces with surrounding soft tissues. in our previous work, titanium plasma immersion ion implantation (piii) technique was applied to modify the carbon-fiber-reinforced polyetheretherketone (cfrpeek) surface, constructing a unique multilevel tio2 nanostructure thus enhancing certain osteogenic properties. however, the interactions between the modified surface and soft-tis ...201626774566
effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility.periodontitis is a chronic inflammatory disease induced by a biofilm that forms on the tooth surface. increased periodontal disease is associated with aging. we investigated the effect of aging on challenge by oral pathogens, examining the host response, colonization, and osteoclast numbers in aged versus young mice. we also compared the results with mice with lineage-specific deletion of the transcription factor foxo1, which reduces dendritic cell (dc) function. periodontitis was induced by ora ...201626762510
effect of low-level laser therapy as an adjuvant in the treatment of periodontitis induced in rats subjected to 5-fluorouracil chemotherapy.this study aimed to assess the effect of multiple sessions of a low-level laser therapy (lllt) adjuvant to scaling and root planing (srp) on the treatment of experimental periodontitis (ep) in rats treated with 5-fluorouracil (5-fu).201626750739
fusobacterium nucleatum, inflammation, and immunity: the fire within human gut.fusobacterium nucleatum is an identified proinflammatory autochthonous bacterium implicated in human colorectal cancer. it is also abundantly found in patients suffering from chronic gut inflammation (inflammatory bowel disease), consequently contributing to the pathogenesis of colorectal cancer. majority of the studies have reported that colorectal tumors/colorectal adenocarcinomas are highly enriched with f. nucleatum compared to noninvolved adjacent colonic tissue. during the course of multis ...201626718210
antimicrobial activity of protamine against oral microorganisms.protamine is an arginine-rich polycationic protein extracted from sperm cells of vertebrates including fishes such as salmon. the purpose of this study was to investigate the suppressive effects of protamine on the growth of oral pathogens for possible usage in dental materials. minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined by the microdilution method. twelve strains of oral viridans streptococci, actinomyces naeslundii, actinomyces odontolyt ...201526699859
high occurrence of fusobacterium nucleatum and clostridium difficile in the intestinal microbiota of colorectal carcinoma patients.colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. several microorganisms have been associated with carcinogenesis, including enterococcus spp., helicobacter pylori, enterotoxigenic bacteroides fragilis, pathogenic e. coli strains and oral fusobacterium. here we qualitatively and quantitatively evaluated the presence of oral and intestinal microorganisms in the fecal microbiota of colorectal cancer patients and healthy controls. seventeen patients (between 49 ...201626691472
fusobacterium nucleatum infection of gingival epithelial cells leads to nlrp3 inflammasome-dependent secretion of il-1β and the danger signals asc and hmgb1.fusobacterium nucleatum is an invasive anaerobic bacterium that is associated with periodontal disease. previous studies have focused on virulence factors produced by f. nucleatum, but early recognition of the pathogen by the immune system remains poorly understood. although an inflammasome in gingival epithelial cells (gecs) can be stimulated by danger-associated molecular patterns (damps) (also known as danger signals) such as atp, inflammasome activation by this periodontal pathogen has yet t ...201626687842
scaling and root planning, and locally delivered minocycline reduces the load of prevotella intermedia in an interdependent pattern, correlating with symptomatic improvements of chronic periodontitis: a short-term randomized clinical evaluate the respective or combinatory efficacy of locally delivered 2% minocycline (mo), and scaling and root planning (srp) by assessing both clinical parameters and the loads of four main periodontal pathogens in treating chronic periodontitis (cp).201526676022
porphyromonas gingivalis initiates a mesenchymal-like transition through zeb1 in gingival epithelial cells.the oral anaerobe porphyromonas gingivalis is associated with the development of cancers including oral squamous cell carcinoma (oscc). here, we show that infection of gingival epithelial cells with p. gingivalis induces expression and nuclear localization of the zeb1 transcription factor, which controls epithelial-mesenchymal transition. p. gingivalis also caused an increase in zeb1 expression as a dual species community with fusobacterium nucleatum or streptococcus gordonii. increased zeb1 exp ...201626639759
polymicrobial oral infection with four periodontal bacteria orchestrates a distinct inflammatory response and atherosclerosis in apoe null mice.periodontal disease (pd) develops from a synergy of complex subgingival oral microbiome, and is linked to systemic inflammatory atherosclerotic vascular disease (asvd). to investigate how a polybacterial microbiome infection influences atherosclerotic plaque progression, we infected the oral cavity of apoe null mice with a polybacterial consortium of 4 well-characterized periodontal pathogens, porphyromonas gingivalis, treponema denticola, tannerealla forsythia and fusobacterium nucleatum, that ...201526619277
antimicrobial photodynamic therapy (apdt) induction of biofilm matrix architectural and bioadhesive implants are commonly used today for the treatment of partially and fully edentulous patients. despite the high success rate they are not resistant to complications and failure due to a variety of problems including peri-implantitis or peri-mucositis due to bacterial biofilm formation on the implant surface. the use of non-surgical and surgical treatment procedure to promote healing in cases with peri-implantitis have limited efficacy. here we studied the ability of photodynamic therapy t ...201626617192
changes in salivary periodontal pathogens after orthodontic treatment: an in vivo prospective analyze the initial changes in salivary levels of periodontal pathogens after orthodontic treatment with fixed appliances.201626606331
hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants.a versatile strategy to endow dental implants with long-term antibacterial ability without compromising the cytocompatibility is highly desirable to combat implant-related infection. silver nanoparticles (ag nps) have been utilized as a highly effective and broad-spectrum antibacterial agent for surface modification of biomedical devices. however, the high mobility and subsequent hazardous effects of the particles on mammalian cells may limit its practical applications. thus, ag nps were immobil ...201526604743
structural and quantitative analysis of a mature anaerobic biofilm on different implant abutment surfaces.the longevity of dental implants depends on the absence of inflammation in the periimplant tissue. similar to teeth, pathogenic bacteria can adhere on implant abutment surfaces and cause periimplant disease and consequently implant loss.201626597465
antibacterial efficacy of a cetylpyridinium chloride-based mouthrinse against fusobacterium nucleatum and in vitro assess the antimicrobial effects of a fluoride-free and alcohol-free mouthrinse containing 0.075% cpc (test rinse, tr) compared with an otherwise-identical cpc-free control rinse (cr).201526591620
the in vitro antimicrobial efficacy of pdt against periodontopathogenic bacteria.periodontitis, an inflammatory disease, is caused by biofilms with a mixed microbial etiology and involves the progressive destruction of the tooth-supporting tissues. a rising number of studies investigate the clinical potential of photodynamic therapy (pdt) as an adjunct during active therapy. the aim of the present review was to evaluate the available literature for the in vitro antimicrobial efficacy of photodynamic therapy focusing on the periodontopathogenic bacteria aggregatibacter actino ...201526580607
genotype-specific regulation of oral innate immunity by t2r38 taste receptor.the bitter taste receptor t2r38 has been shown to regulate mucosal innate immune responses in the upper airway epithelium. furthermore, snps in t2r38 influence the sensitivity to 6-n-propylthiouracil (prop) and are associated with caries risk/protection. however, no study has been reported on the role of t2r38 in the innate immune responses to oral bacteria. we hypothesize that t2r38 regulates oral innate immunity and that this regulation is genotype-specific. primary gingival epithelial cells c ...201526552761
humoral immune responses to periodontal pathogens in the elderly.elderly people are thought to be more susceptible to periodontal disease due to reduced immune function associated with aging. however, little information is available on the nature of immune responses against putative periodontal pathogens in geriatric patients. the purpose of this study was to evaluate the serum igg antibody responses to six periodontal pathogens in geriatric subjects.201526550526
complete genome sequences and analysis of the fusobacterium nucleatum subspecies animalis 7-1 bacteriophage ɸfunu1 and ɸfunu2.fusobacterium nucleatum is a strictly anaerobic, gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. the species is unusual in its phenotypic and genotypic heterogeneity, with some strains demonstrating a more virulent phenotype than others; however, as yet the genetic basis for these differences is not understood. bacteriophage are known to contribute to the virulence ph ...201626545740
the effect of bamboo leaf extract solution and sodium copper chlorophyllin solution on growth and volatile sulfur compounds production of oral malodor associated some anaerobic periodontal bacteria.bamboo leaf extract solution (bles) and sodium copper chlorophyllin solution (sccs) are known for their anti-oxidant activities. oral malodor is often related with periodontal pathogens. the present study was undertaken to investigate the anti-bacterial effect of both bles and sccs on anaerobic periodontal bacteria producing oral malodorous volatile sulfur compounds (vsc).201526538799
proteomic profiling of host-biofilm interactions in an oral infection model resembling the periodontal pocket.periodontal infections cause inflammatory destruction of the tooth supporting tissues. we recently developed a dynamic, in vitro periodontal organotypic tissue model in a perfusion bioreactor system, in co-culture with an 11-species subgingival biofilm, which may recapitulate early events during the establishment of periodontal infections. this study aimed to characterize the global proteome regulations in this host-biofilm interaction model. semi-quantitative shotgun proteomics were applied for ...201526525412
periodontal pathogens and risk of incident cancer in postmenopausal females: the buffalo osteoperio study.extraoral translocation of oral bacteria may contribute to associations between periodontal disease and cancer. the associations among the presence of three orange-complex periodontal pathogens (fusobacterium nucleatum, prevotella intermedia, and campylobacter rectus), two red-complex periodontal pathogens (porphyromonas gingivalis and tannerella forsythia), and cancer risk were investigated.201626513268
randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin.the aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (rmgic)], ledtb [photodynamic antimicrobial chemotherapy (pact) with light-emitting diode associated with toluidine blue solution and rmgic], and lmb ...201526502235
subgingival plaque in periodontal health antagonizes at toll-like receptor 4 and inhibits e-selectin expression on endothelial cells.the ability of the subgingival microbial community to induce an inappropriate inflammatory response ultimately results in the destruction of bone and gingival tissue. in this study, subgingival plaque samples from both healthy and diseased sites in the same individual were obtained from adults with chronic periodontitis and screened for their ability to either activate toll-like receptor 2 (tlr2) or tlr4 and to antagonize tlr4-specific activation by agonist, fusobacterium nucleatum lps. subgingi ...201526483407
antimicrobial effectiveness of cetylpyridinium chloride and zinc chloride-containing mouthrinses on bacteria of halitosis and peri-implant clarify the antimicrobial efficacy of zinc chloride (zncl2) and cetylpyridinium chloride (cpc) by testing their impact on the growth of seven bacterial strains known to be involved in the pathophysiology of both peri-implant disease and halitosis-staphylococcus aureus, streptococcus mutans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, treponema denticola, and tannerella forsythia.201526478974
secreted adenosine triphosphate from aggregatibacter actinomycetemcomitans triggers chemokine response.extracellular atp (eatp) is an important intercellular signaling molecule secreted by activated immune cells or released by damaged cells. in mammalian cells, a rapid increase of atp concentration in the extracellular space sends a danger signal, which alerts the immune system of an impending danger, resulting in recruitment and priming of phagocytes. recent studies show that bacteria also release atp into the extracellular milieu, suggesting a potential role for eatp in host-microbe interaction ...201626470857
oral microbiota, periodontal status, and osteoporosis in postmenopausal females.alterations in hormone levels during menopause decrease bone density and may worsen oral health, favoring the growth of periodontal pathogens, whose detection could improve the diagnosis of periodontitis. the aim of this study is to detect and quantify the main periodontal pathogens in the oral microbiota of postmenopausal females and to explore the relationship between clinical and periodontal parameters.201626469811
incorporation of staphylococci into titanium-grown biofilms: an in vitro "submucosal" biofilm model for peri-implantitis.staphylococcus spp. are postulated to play a role in peri-implantitis. this study aimed to develop a "submucosal" in vitro biofilm model, by integrating two staphylococci into its composition.201526461083
antibacterial activity of curcumin against periodontopathic bacteria.curcumin is a polyphenol extracted from root of turmeric and known to possess multifunctional properties, including antibacterial activity. although previous studies have investigated the effects of curcumin on microorganisms, available knowledge on the effects of curcumin on periodontopathic bacteria is still limited. in this study, the antibacterial effect of curcumin on periodontopathic bacteria is investigated, particularly porphyromonas gingivalis.201626447754
[noma/cancrum oris: a neglected disease].noma is an aggressive orofacial gangrenous pathology that damages hard and soft tissues of the mouth and the face. throughout the centuries it has been present around the globe, but nowadays it has practically disappeared from developed countries and mainly affects children from the most disadvantaged places, especially in africa. noma disease is a multifactorial process; malnutrition, debilitating diseases (bacterial or viral systemic diseases, hiv-associated immunosuppression, etc.) and intrao ...201526437752
robust species taxonomy assignment algorithm for 16s rrna ngs reads: application to oral carcinoma samples.usefulness of next-generation sequencing (ngs) in assessing bacteria associated with oral squamous cell carcinoma (oscc) has been undermined by inability to classify reads to the species level.201526426306
microbiological effects and recolonization patterns after adjunctive subgingival debridement with er:yag laser.the objective of this study was to assess the microbiological effects and recolonization patterns after non-surgical periodontal treatment protocol based on the adjunctive use of erbium-doped yttrium aluminium garnet (er:yag) laser.201626419675
slowly progressive lemierre's syndrome with orbital pain and exophthalmos.lemierre's syndrome is an oropharyngeal bacterial infection characterized by rapidly progressive septic thrombophlebitis of the internal jugular vein. a lack of appropriate antibiotic therapy can be life-threatening. we describe the case of a 39-year-old man with lemierre's syndrome who presented with long-lasting orbital pain and acute exophthalmos 6 weeks after initial infection. this report is to help clinicians consider the diagnosis of lemierre's syndrome when encountering a patient with lo ...201626419364
long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in evaluate in long-term periods the destruction of periodontal tissues and bacterial colonization induced by oral gavage with periodontopathogens or ligature experimental periodontal disease models.201626411857
metagenomic analysis of faecal microbiome as a tool towards targeted non-invasive biomarkers for colorectal evaluate the potential for diagnosing colorectal cancer (crc) from faecal metagenomes.201726408641
fusobacterium nucleatum osteomyelitis in 3 previously healthy children: a case series and review of the literature.fusobacterium nucleatum is a rare cause of monomicrobial osteomyelitis in children. we describe the cases of 3 children with f. nucleatum osteomyelitis of the distal femoral epiphysis with concurrent septic arthritis and review 6 other cases reported in the literature. our report emphasizes the importance of maintaining a high index of suspicion for anaerobic osteomyelitis, given its atypical presentation and the unique growth requirements of anaerobic bacteria.201526407282
"messieurs, c'est les microbes qui auront le dernier mot": gentlemen, it is the microbes who have the last word (louis pasteur)-fusobacterium nucleatum protect tumors from killing by immune cells.fusobacterium nucleatum is present in colon cancers where it was shown to generate a proinflammatory microenvironment that supports colorectal neoplasia progression. remarkably, alongside with proinflammatory stimulation, fusobacteria also inhibit cytotoxicity of immune cells. thus, it appears as if tumors exploit fusobacteria to generate a favorable proinflammatory and anti-cytotoxic microenvironment.201526405611
berberine may rescue fusobacterium nucleatum-induced colorectal tumorigenesis by modulating the tumor microenvironment.accumulating evidence links colorectal cancer (crc) with the intestinal microbiota. however, the disturbance of intestinal microbiota and the role of fusobacterium nucleatum during the colorectal adenoma-carcinoma sequence have not yet been evaluated.201526397137
the gut microbial community in metabolic syndrome patients is modified by diet.intestinal microbiota changes may be involved in the development of metabolic syndrome (mets), which is a multicomponent disorder frequently associated with obesity. the aim of this study was to test the effect of consuming two healthy diets: a mediterranean diet and a low-fat high-carbohydrate diet, for 2years in the gut microbiota of mets patients and those in the control group. we analyzed the differences in the bacterial community structure between the groups after 2years of dietary interven ...201626376027
evaluation of the antibacterial effect of silver nanoparticles on guided tissue regeneration membrane colonization--an in vitro study.the aim of this in vitro study was to investigate the colonization and penetration of specific bacteria on nanosilver-impregnated gtr (guided tissue regeneration) membranes.201526373223
periodontal pathogens invade gingiva and aortic adventitia and elicit inflammasome activation in αvβ6 integrin-deficient mice.the american heart association supports an association between periodontal diseases and atherosclerosis but not a causal association. this study explores the use of the integrin β6(-/-) mouse model to study the causality. we investigated the ability of a polymicrobial consortium of porphyromonas gingivalis, treponema denticola, tannerella forsythia, and fusobacterium nucleatum to colonize the periodontium and induce local and systemic inflammatory responses. polymicrobially infected itgβ6(-/-) m ...201526371120
identification of an antibacterial protein by functional screening of a human oral metagenomic library.screening of a bacterial artificial chromosome (bac) library containing metagenomic dna from human plaque and saliva allowed the isolation of four clones producing antimicrobial activity. three of these were pigmented and encoded homologues of glutamyl-trna reductase (glutr), an enzyme involved in the c5 pathway leading to tetrapyrole synthesis, and one clone had antibacterial activity with no pigmentation. the latter contained a bac with an insert of 15.6 kb. initial attempts to localize the ge ...201526347298
significant increase of oral bacteria in the early pregnancy period in japanese women.oral microflora during pregnancy is critical to oral health care in the mother and her child. we examined the changes in the oral microbiota between pregnancy and nonpregnancy periods.201726345599
endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation.this study aims to assess the potential of a mixture of three antibiotics (trevitamix, tvm) as an intracanal dressing to disinfect the outer root surface by applying a new in vitro model.201626323501
effects of surface reaction-type pre-reacted glass ionomer on oral biofilm formation of streptococcus gordonii.streptococcus gordonii, a bacterium involved in the initial colonization of tooth surfaces, contributes to dental biofilm formation and is an important cause of infective endocarditis. this study aimed to investigate the influence of surface reaction-type pre-reacted glass ionomer (s-prg) filler on oral bacterial growth and aggregation of s. gordonii. the effect of various concentrations of s-prg eluate on the growth and the biofilm formation of s. gordonii and other oral microorganisms (strepto ...201626319990
sialic acid transporter nant participates in tannerella forsythia biofilm formation and survival on epithelial cells.tannerella forsythia is a periodontal pathogen implicated in periodontitis. this gram-negative pathogen depends on exogenous peptidoglycan amino sugar n-acetylmuramic acid (nam) for growth. in the biofilm state the bacterium can utilize sialic acid (neu5ac) instead of nam to sustain its growth. thus, the sialic acid utilization system of the bacterium plays a critical role in the growth and survival of the organism in the absence of nam. we sought the function of a t. forsythia gene annotated as ...201626318875
fusobacterium nucleatum in colorectal carcinoma tissue and patient prognosis.accumulating evidence links the intestinal microbiota and colorectal carcinogenesis. fusobacterium nucleatum may promote colorectal tumour growth and inhibit t cell-mediated immune responses against colorectal tumours. thus, we hypothesised that the amount of f. nucleatum in colorectal carcinoma might be associated with worse clinical outcome.201626311717
synergic phototoxic effect of visible light or gallium-arsenide laser in the presence of different photo-sensitizers on porphyromonas gingivalis and fusobacterium nucleatum.according to the development of resistant strains of pathogenic bacteria following treatment with antimicrobial chemotherapeutic agents, alternative approaches such as lethal photosensitization are being used. the aim of this study was to evaluate the effect of visible light and laser beam radiation in conjugation with three different photosensitizers on the survival of two main periodontopathogenic bacteria including porphyromonas gingivalis and fusobacterium nucleatum in different exposure per ...201526288621
oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients.the objective of this study was to identify the dna of oral bacteria in placental samples from women with and without periodontitis who had or had not had preterm births and/or low birthweight (pb/lbw) neonates.201526259070
in vitro activity of taurolidine gel on bacteria associated with periodontitis.the purpose of this in vitro study was to determine the antimicrobial activity of two different taurolidine gel formulations in comparison with minocycline microspheres.201626254142
microbiologic and clinical findings of implants in healthy condition and with compare implants in healthy conditions and implants with peri-implantitis with regard to their clinical parameters and the microbiologic composition at the peri-implant sulcus, inside the implant connection, and the gingival sulcus of neighboring teeth.201626252036
lethal effect of blue light-activated hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on the viability of porphyromonas gingivalis and fusobacterium nucleatum.recently, photodynamic therapy (pdt) has been introduced as a new modality in oral bacterial decontamination. current research aims to evaluate the effect of photodynamic killing of visible blue light in the presence of hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on porphyromonas gingivalis associated with periodontal bone loss and fusobacterium nucleatum associated with soft tissue inflammation.201526246690
inactivating effects of the lactoperoxidase system on bacterial lyases involved in oral malodour production.the main components of oral malodour have been identified as volatile sulfur compounds (vscs), including hydrogen sulfide (h(2)s) and methyl mercaptan (ch(3)sh). the lactoperoxidase (lpo) system (consisting of lpo, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities against some oral bacteria in vitro and suppressive effects on vscs in mouth air in a clinical trial. here, we examined the in vitro effects of the lpo system on the activities of the ba ...201526242770
in vitro growth-inhibitory effect of ethanol gras plant and supercritical co₂ hop extracts on planktonic cultures of oral pathogenic microorganisms.conventional chemical antiseptics used for treatment of oral infections often produce side-effects, which restrict their long-term use. plants are considered as perspective sources of novel natural antiseptics. however, little is still known about their inhibitory properties against oral pathogens. the objective of this study was to test in vitro antimicrobial activities of generally recognized as safe (gras) species against planktonic cultures of cariogenic, periodontal and candidal microorgani ...201526232134
efficacy of a novel antimicrobial peptide against periodontal pathogens in both planktonic and polymicrobial biofilm states.streptococcus gordonii, fusobacterium nucleatum and porphyromonas gingivalis represent the early, middle and late colonizers of the bacterial accretion in dental plaque biofilms. these sessile communities constitute a protected mode of growth that promotes survival in a hostile environment. this study describes a novel and unrecognized role for a synthetic cationic antimicrobial peptide, nal-p-113, which inhibits and kills periodontal bacteria in planktonic state, inhibits the formation of biofi ...201526210284
wild blueberry (vaccinium angustifolium ait.) polyphenols target fusobacterium nucleatum and the host inflammatory response: potential innovative molecules for treating periodontal diseases.blueberries contain significant amounts of flavonoids to which a number of beneficial health effects in humans have been associated. the present study investigated the effect of a polyphenol-rich lowbush blueberry (vaccinium angustifolium ait.) extract on the two main etiologic components of periodontitis, a multifactorial disorder affecting the supporting structures of the teeth. phenolic acids, flavonoids (flavonols, anthocyanins, flavan-3-ols), and procyanidins made up 16.6, 12.9, and 2.7% of ...201526207764
detection of subgingival periodontal pathogens--comparison of two sampling strategies.the aim of the study is to compare detection frequency of periodontal pathogens in patients with aggressive/severe chronic periodontitis using pooled plaque samples from the deepest pockets per quadrant/per sextant.201626193958
advanced caries microbiota in teeth with irreversible pulpitis.bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. this study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis.201526187422
fusobacterium nucleatum and t cells in colorectal carcinoma.evidence indicates a complex link between gut microbiome, immunity, and intestinal tumorigenesis. to target the microbiota and immunity for colorectal cancer prevention and therapy, a better understanding of the relationship between microorganisms and immune cells in the tumor microenvironment is needed. experimental evidence suggests that fusobacterium nucleatum may promote colonic neoplasia development by downregulating antitumor t cell-mediated adaptive immunity.201526181352
effects of hangeshashinto on growth of oral microorganisms.oral mucositis (om) in cancer patients induced by chemotherapy or radiotherapy has a significant impact on quality of life, and causes considerable morbidity. oral microorganisms are likely to intensify the inflammatory process and aggravate the formation of ulcers. hangeshashinto (hst), a japanese kampo medicine, has been reported to be effective when used as a gargle for the treatment of om. to clarify the effects of hst on oral microorganisms, we assessed its antimicrobial activity against 27 ...201526170876
[the efficacy of cold-light tooth bleaching on the formation of bacteria biofilm on the enamel surfaces].to evaluate the efficacy of beyond cold-light tooth bleaching on the formation of main cariogenic bacteria biofilm on enamel surfaces.201526166513
antibacterial activity of commercially available plant-derived essential oils against oral pathogenic bacteria.this work investigated the antibacterial activity of 15 commercially available plant-derived essential oils (eos) against a panel of oral pathogens. the broth microdilution method afforded the minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs) of the assayed eos. the eo obtained from cinnamomum zeylanicum (lauraceae) (cz-eo) displayed moderate activity against fusobacterium nucleatum (mic and mbc = 125 μg/ml), actinomyces naeslundii (mic and mbc = 125 μg/ml), ...201626165725
periodontal pathogens porphyromonas gingivalis and fusobacterium nucleatum promote tumor progression in an oral-specific chemical carcinogenesis model.oral squamous cell carcinoma (oscc) is a lethal disease whose incidence is increasing. epidemiologic studies demonstrate an association between periodontitis and oral cancer, and periodontal pathogens are implicated in the pathogenesis of numerous disorders, including rheumatoid arthritis, cardiovascular diseases, diabetes and gastrointestinal malignancies. nevertheless, a causal role for periodontal pathogens in oscc has not been shown, partly due to the lack of an appropriate animal model. her ...201526158901
cellular components mediating coadherence of candida albicans and fusobacterium nucleatum.candida albicans is an opportunistic fungal pathogen found as part of the normal oral flora. it can be coisolated with fusobacterium nucleatum, an opportunistic bacterial pathogen, from oral disease sites, such as those involved in refractory periodontitis and pulp necrosis. the physical coadherence between these 2 clinically important microbes has been well documented and suggested to play a role in facilitating their oral colonization and colocalization and contributing to polymicrobial pathog ...201526152186
coinfection with fusobacterium nucleatum can enhance the attachment and invasion of porphyromonas gingivalis or aggregatibacter actinomycetemcomitans to human gingival epithelial cells.this study was conducted to investigate effects of coinfection of porphyromonas gingivalis (p. gingivalis) or aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) with fusobacterium nucleatum (f. nucleatum) on their adhering and invasive capacity to human gingival epithelial cells as well as the expression of interleukin-8 (il-8) and human beta-defensin-2 (hbd-2) in human gingival epithelial cells.201526143497
periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased dominican adults.the objective of this study was to compare the periodontopathogen prevalence and tetracycline resistance genes in dominican patients with different periodontal conditions.201526121972
the biofilm community-rebels with a cause.oral biofilms are one of the most complex and diverse ecosystem developed by successive colonization of more than 600 bacterial taxa. development starts with the attachment of early colonizers such as actinomyces species and oral streptococci on the acquired pellicle and tooth enamel. these bacteria not only adhere to tooth surface but also interact with each other and lay foundation for attachment of bridging colonizer such as fusobacterium nucleatum followed by late colonizers including the re ...201526120510
fusobacterium nucleatum: a rare cause of pyogenic liver abscess.while pyogenic liver abscesses are uncommon, they are associated with significant mortality and morbidity. most pyogenic liver abscesses are polymicrobial and are caused by enteric bacteria and anaerobes. rarely, mono-microbial infections may occur, typically in immunocompromised individuals. we report the unusual case of a 69 year-old immunocompetent female who developed a pyogenic liver abscess due to fusobacterium nucleatum infection, likely from a dental source. poor oropharyngeal hygiene se ...201526101735
determination of antibacterial activity of green coffee bean extract on periodontogenic bacteria like porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum and aggregatibacter actinomycetemcomitans: an in vitro study.the aim of this study was to evaluate the antibacterial activity of pure green coffee bean extract on periodonto pathogenic bacteria porphyromonas gingivalis (pg), prevotella intermedia (pi), fusobacterium nucleatum (fn) and aggregatibacter actinomycetemcomitans (aa).201726097349
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