synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens.our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ...19921324304
role of porphyromonas gingivalis 40-kda outer membrane protein in the aggregation of p. gingivalis vesicles and actinomyces viscosus.porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with actinomyces viscosus cells. a 40-kda outer membrane protein (omp)-coding gene from p. gingivalis was cloned and the protein was found to be localized in these vesicles. the recombinant 40-kda omp did not show aggregation activity. however, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of p. gingivalis vesicles with a. viscosus cell ...19921329700
effect of clavulanic acid, sulbactam and tazobactam on three different beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum.the effect of three beta-lactamase inhibitors, clavulanic acid, sulbactam and tazobactam used in clinical practice were compared for their activity against purified beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. the enzymes from b. uniformis and c. butyricum were produced in fermenters under controlled growth conditions and the enzyme from f. nucleatum was produced in batch cultures. purification of the beta-lactamases was achieved by anion-exchang ...19921331018
correlation of haemagglutination activity with trypsin-like protease activity of porphyromonas gingivalis.porphyromonas gingivalis is a gram-negative anaerobic bacterium associated with various forms of periodontal disease. several characteristics of p. gingivalis are thought to contribute to its pathogenicity; these include haemagglutination and trypsin-like protease activity. previous studies suggest an association between haemagglutination and trypsin-like protease activity of p. gingivalis. to investigate this, two complementary quantitative experimental approaches were taken. five independent m ...19921332663
sensitization of oral bacteria in biofilms to killing by light from a low-power laser.biofilms of streptococcus sanguis, porphyromonas gingivalis, fusobacterium nucleatum and actinobacillus actinomycetemcomitans were prepared on the surfaces of agar plates and a number of compounds were screened for their ability to sensitize bacteria in these biofilms to killing by light from a 7.3 mw helium/neon (he/ne) laser. toluidine blue o and methylene blue enabled detectable killing of all four target organisms after exposure to he/ne light for 30 s. aluminium disulphonated phthalocyanine ...19921334649
bacterial concentration fluorescence immunoassay (bcfia) for the detection of periodontopathogens in plaque.a bacterial concentration fluorescence immunoassay (bcfia) was developed to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilized fluorescent-tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative bacteria. microorganisms identified in plaque using the bcfia included actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. the immunoassay procedure involved com ...19921336047
dna probe and enzyme analysis in periodontal diagnostics.recent research in periodontology has indicated that a finite number of bacterial species are associated with periodontal disease. this has generated the hypothesis that periodontal disease is essentially an infection due to one or more of the putative periodontopathogens; i.e., the specific plaque hypothesis (sph). this microbial specificity paradigm has already changed the way researchers and some clinicians view periodontal disease. the clinician must heed several cautions signs, however, if ...19921336048
antibacterial efficacy of root canal medicaments.the antibacterial efficacy of four endodontic medicaments was studied in vitro. calcium hydroxide (calacept), camphorated parachlorophenol (cpcp), cresophene, and 2% iodine-potassium iodide (iki) were tested against four anaerobic microorganisms, streptococcus mutans, peptostreptococcus anaerobius, porphyromonus gingivalis and fusobacterium nucleatum, for 10 or 15 min. iki was effective only against f. nucleatum and p. gingivalis, and the others were effective against all four microorganisms.19921337555
effect of beta-lactamase inhibitors on beta-lactamases from anaerobic bacteria.three beta-lactamase inhibitors in clinical use--clavulanic acid, sulbactam and tazobactam--were investigated for their activity on beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. purification of the beta-lactamases was carried out by anion-exchange chromatography, gel filtration and fplc. the inactivation of beta-lactamase activity was determined spectrophotometrically with nitrocefin as substrate. various concentrations of the inhibitors were prei ...19921338315
release of endogenous noradrenaline from the vascularly perfused rat stomach in vitro: modulation by pre- and postsynaptic adrenoceptors.we developed an experimental in vitro model to detect a very small amount of endogenous noradrenaline (na) released from the rat gastric sympathetic nerve terminals. the stomach was perfused via celiac artery with modified krebs-ringer solution containing 10 mm pargyline and 0.1% bovine serum albumin at a constant flow of 4 ml/min. the right greater splanchnic (spl) nerve (preganglionic nerve of the gastric sympathetic nerve) was stimulated electrically with square-wave pulses of 2 msec duration ...19921346643
structure of holo-chaperonin studied with electron microscopy. oligomeric cpn10 on top of two layers of cpn60 rings with two stripes each.a structural model of holo-chaperonin, known as a protein-folding control protein comprising 60 kda (cpn60) and 10 kda polypeptides (cpn10), is proposed based on the electron microscopic images of holo-chaperonin from thermus thermophilus and cpn60 from paracoccus denitrificans. isolated paracoccus cpn60 shows very similar images to those of escherichia coli tetradecameric cpn60, a seven-membered ring in the top view and a rectangular shape with four stripes in the side view. however, a small nu ...19921347504
detubularization-induced contractile response change of the ileum following ileocystoplasty.we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ...19921351953
immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium.immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ...19921354674
cd45ra and cd45ro positive cd4 cells in human peripheral blood and periodontal disease tissue before and after stimulation with periodontopathic bacteria.flow cytometric analysis was used to examine naive and primed or memory cd4 cells extracted from periodontal lesions compared with cells from peripheral blood of healthy subjects before and after stimulation with the periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. in peripheral blood, approximately 60% and 40% of cd4 cells were cd45ro+ and cd45ra+ respectively at day 0. phytohaemagglutinin (pha) induced cd45ro expression on almost 100% of cd4 cells. however, p. g ...19921356262
different responses in b cells induced by porphyromonas gingivalis and fusobacterium nucleatum.a phenotypic study had shown that gingival b cells respond differently to two periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. further investigation now shows a reduction in the percentage of ki-67 + t cells in cultures of gingival and peripheral blood mononuclear cells stimulated with p. gingivalis for 3 and 6 days, respectively, but no suppression of ki-67 expression in b cells in response to either p. gingivalis or f. nucleatum. depletion studies of cultures of ...19921359860
sensitization of oral bacteria to killing by low-power laser radiation.twenty-seven compounds were screened for their ability to sensitize streptococcus sanguis to killing by light from a 7.3-mw helium/neon (hene) laser. bacteria were mixed with various concentrations of the test compounds, spread over the surfaces of agar plates, and then exposed to light from the hene laser for various time periods. the plates were then incubated and examined for zones of inhibition. those compounds found to be effective photosensitizers were then tested against porphyromonas gin ...19921369193
characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis.alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ...19921370473
dinucleotide (ca/gt) repeat polymorphism in intron 17b of the cystic fibrosis transmembrane conductance regulator (cftr) gene.we describe a polymorphic microsatellite (ivs17bca) in intron 17b of the cystic fibrosis transmembrane conductance regulator (cftr) gene. it consists of an 11 to 20 ca/gt dinucleotide repeat, located 424 bp from exon 17b.19921370810
biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis.the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ...19921370931
the establishment of human t cell lines reactive with specific periodontal bacteria.human t cell lines (tcls) were obtained by stimulation of peripheral blood mononuclear cells (pbmcs) with the 2 periodontopathic bacteria, porphyromonas gingivalis fdc-381 and fusobacterium nucleatum fdc-263. after the first round of stimulation and rest, the cells responded specifically to the bacteria originally used to establish each line. throughout the culture period, the responsiveness of each of the tcls to their specific bacteria increased. phenotypic analysis of the tcls revealed hetero ...19921383912
[lemierre's syndrome with splenic abscesses].a week after onset of a pharyngo-tonsillitis a previously healthy 23-year-old man developed high fever (41.4 degrees c), leukocytosis (12,200/microliters) with marked shift to the left, thrombocytopenia (86,000/microliters) and increased transaminases (got 83 u/l, gpt 113 u/l). chest x-ray film suggested intrapulmonary abscesses with left-sided pleural effusion. the suspected diagnosis of "post-tonsillitis" septicaemia (lemierre's syndrome) was confirmed by demonstrating anaerobic, fusiform, gra ...19921396137
identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases.activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ...19921398963
longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases.fifty-one subjects (16-61 years old) with evidence of prior destructive periodontal disease were monitored clinically and immunologically at bi-monthly intervals for up to 5 yr. periodontal disease activity, determined as new attachment loss, was detected in 33 of these subjects. only 4 of 51 subjects failed to show an elevated serum antibody level to any of the 18 subgingival species tested. the antibody level threshold established for periodontally healthy subjects was exceeded most often in d ...19921403580
experimentally induced infection by oral anaerobic microorganisms in a mouse model.a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ...19921408362
2-hydroxyglutaryl-coa dehydratase from fusobacterium nucleatum (subsp. nucleatum): an iron-sulfur flavoprotein.anaerobically prepared cell-free extracts from fusobacterium nucleatum contain 2-hydroxyglutaryl-coa dehydratase with a specific activity of 20 nkat mg-1. the enzyme was purified 24-fold to a specific activity of 480 nkat mg-1 by anion exchange chromatography, gel filtration and chromatography on blue-sepharose. the activity of the purified enzyme was strictly dependent on the reductant ti(iii)citrate and stimulated 25-fold by 0.15 mm atp and 5 mm mgcl2. atp is hydrolysed to adp during incubatio ...19921417419
mannitol does not affect energy metabolism in forebrain ischemia.mannitol has a beneficial effect on ischemic injury following a short-duration forebrain ischemic insult in rats. using the same animal model, we attempted to show that this effect of mannitol could be traced (via an improvement in cerebral blood flow) to a tempering of the collapse in the high-energy phosphates that occurs during the insult. a 10 min ischemic insult was induced by bilateral carotid artery occlusion followed by a reduction of the blood pressure to 50 mmhg through removal of bloo ...19921421096
complement activation by lipopolysaccharides purified from gram-negative bacteria isolated from infected root canals.the purpose of the present study was to investigate the complement activation by lipopolysaccharides purified from porphyromonas (bacteroides) endodontalis, veillonella parvula, and fusobacterium nucleatum isolated from infected root canals. the rate of consumption of the c3t component of the complement increased remarkably when lipopolysaccharides of more than 10 micrograms were added.19921437068
comparison of the effects of galactose and glucose on the ph responses of human dental plaque, salivary sediment and pure cultures of oral bacteria.comparisons made in dental plaque in vivo demonstrated that galactose produces a significantly smaller decrease in ph than does glucose. in vitro studies with plaque, salivary sediment and pure cultures of oral bacteria done in the absence of intraoral factors such as flowing saliva confirmed this lesser acidogenicity of galactose. pure culture showed that most of the bacteria tested produce a moderate to large decrease with glucose but only a few do so with galactose; most produced a moderate t ...19921444892
a comparative study of randomly amplified polymorphic dna analysis and conventional phage typing for epidemiological studies of listeria monocytogenes isolates.the analysis of rapd profiles generated by pcr with a single 10-mer, hlwl74, was compared to bacteriophage susceptibility data for epidemiological typing of listeria monocytogenes strains. a total of 104 l. monocytogenes strains was screened, all from serogroup 1 or serotype 4b. of these, 53 had been isolated during 6 different listeriosis outbreaks. the remaining 51 strains were chosen randomly from our collection. a total of 38 rapd types were observed, although each epidemic group of strains ...19921448626
immunohistochemical localization of gangliosides in enu-induced rat glioma.biochemical studies have indicated that the structurally simple gangliosides, including gd3 and gm3, are major glycolipid components of glioma tissues. in order to clarify the localization of the gangliosides in ethylnitrosourea-induced rat glioma, an immunohistochemical study was performed using antibodies against gm1, gm3, and gd3. the results obtained in normal fetus, newborn, and adult rat brain, and also in human glioma, were compared. in fetal and newborn rat brain, gd3 was present mainly ...19921449052
an unrecognized etiology for pyogenic hepatic abscesses in normal hosts: dental disease.cryptogenic pyogenic hepatic abscesses are a diagnosis of exclusion. we have identified two patients with severe dental disease at the time of the diagnosis of their liver abscess. in both cases, oral flora was cultured from the abscess. unlike a previous report, both patients were immunocompetent. when compared with a group of patients with liver abscesses and diverticulitis, two differences were found. in contrast to the single abscesses seen in 10 of 10 patients with diverticulitis, the patie ...19921449134
cellular fatty acids in fusobacterium species as a tool for identification.identification of fusobacteria from clinical specimens currently requires analysis of metabolic end products by gas-liquid chromatography in addition to certain biochemical and enzymatic tests because of the relative biochemical inactivity of these bacteria. even the finding of pointed, thin gram-negative cells on gram-stained slides can no longer be relied on for identification of fusobacterium nucleatum, since at least four other species of fusobacteria have been seen to exhibit similar morpho ...19921452706
phenolic constituents in erythrina x bidwilli and their activity against oral microbial organisms.five flavonoid compounds, including two new isoflavanones, were isolated from the root bark of erythrina x bidwilli. their structures were determined to be 2,10-di(gamma,gamma-dimethylallyl)-3,9-dihydroxypterocarpan (erythrabyssin ii), 6,8-di(gamma,gamma-dimethylallyl)-7,2',4'-trihydroxyisoflavanone (bidwillon a), 8-gamma,gamma-dimethylallyl-2',4'-dihydroxy-[6",6"-di-methylpyrano - (2",3":7,6)]isoflavanone (bidwillon b), 8-gamma,gamma-dimethylallyl-7,4'-dihydroxyisoflavone (8-gamma,gamma-dimethy ...19921464104
clonal dental pulp cells (rdp4-1, rpc-c2a) synthesize and secrete osteopontin (spp1, 2ar).dental pulp cells play an important role in maintaining dental mineralized tissue throughout life. supplementary mineralization such as reparative dentin and pulp stone frequently occurs after primary dentin formation. dental pulp cells are thought to be closely associated with such mineralization. we found that clonal rat dental pulp cells, rdp4-1 and rpc-c2a, produce and secrete osteopontin, but do not synthesize phosphophoryn which is a major noncollagenous protein found in dentin. the dental ...19921472061
rat sertoli and spermatogenic cells express a similar gene, and its product is antigenically related to an outer dense fiber-associated protein.we have previously reported that a heterodimeric protein secreted by rat sertoli cells is antigenically related to a protein associated with outer dense fibers of the sperm tail. therefore, we have explored the possibility that sertoli and spermatogenic cells express a similar gene encoding a homologous protein. a sertoli cell heterodimeric protein cdna probe recognizes specific mrna in pachytene and round spermatids fractionated by centrifugal elutriation; however, this specific mrna was less p ...19921472368
hiv education and health education in the united states: a national survey of local school district policies and determine the extent to which hiv education and health education policies and practices are required by school districts in the united states, a national probability sample of public school districts was surveyed by mail in 1990. of 2,150 districts selected, 78.1% responded. hiv education was required by 66.9% of districts. of these, the percentage requiring hiv education increased by grade level from 29.7% in kindergarten to 82.3% in 7th grade, then declined to 37.3% by 12th grade. districts ...19921479838
[pneumococcus-induced septicemia in normal and splenectomized rabbits].a new rabbit model to study the consequences of splenectomy in host resistance to induced pneumococcal septicaemia is presented. a simple, fast, non-invasive and complication-free technique of bacterial inoculation into the rabbit airways, using transcutaneous cannulation of the trachea, is also described. sixteen normal and 24 splenectomised animals were used. the optimal dose of pneumonococcus (serotype b, type iii) was found to be 1 x 10(7) organisms. the above dose of bacteria given transtra ...19921479858
susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase.the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ...19921481764
expression of small heat shock proteins by the third-stage larva of brugia pahangi.changes in proteins synthesised by the infective third-stage larvae (l3) of the filarial nematode brugia pahangi were examined with respect to the temperature shift encountered by the parasite as it migrates from insect to mammal, and the presence of serum in the culture medium. while the synthesis of a number of polypeptides is regulated by the temperature shift of the l3 from 28 degrees c to 37 degrees c in vitro, there is no evidence that serum has any significant effect on protein synthesis. ...19921484547
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
relationship between oral gram-negative anaerobic bacteria in saliva of the mother and the colonization of her edentulous infant.various gram-negative anaerobic bacterial species can be detected in the oral flora of edentulous infants. however, knowledge of the initial infection source is still scarce. thirty caucasian mothers (mean age 30 years) and their edentulous infants (mean age 3 months) were examined for the possible similarity of the oral gram-negative anaerobic flora. paraffin-stimulated saliva was collected from the mothers. a pooled swab sample from mucosal surfaces and unstimulated saliva were collected from ...19921494450
coaggregation studies of the eubacterium species.eubacterium species are gram-positive anaerobic rods that are frequently isolated from subgingival plaque of periodontal pockets. five eubacterium species were tested for their ability to coaggregate with 33 oral bacterial strains. using visual and turbidimetric assays, coaggregation was observed among eubacterium brachy, eubacterium nodatum, eubacterium alactolyticum and eubacterium limosum strains only when tested with fusobacterium nucleatum strains; eubacterium saburreum displayed only weak ...19921494452
characterization of shared antigens of fusobacterium nucleatum and fusobacterium necrophorum.fusobacterium nucleatum and fusobacterium necrophorum are gram-negative, non-spore-forming anaerobic rods, frequently isolated from the normal flora and diseased lesions of the human oral cavity, gastrointestinal and genitourinary tracts. f. necrophorum is also known to be an animal pathogen. studies were undertaken with rabbit anti-f. nucleatum sera and with human adult periodontitis (ap) sera that demonstrated the sharing of antigens between the two species. immunodiffusion and immunoelectroph ...19921494453
the breakdown and utilization of peptides by strains of fusobacterium has been claimed that most strains of fusobacterium nucleatum require peptides rather than free amino acids for growth. in contrast, we have shown that, under continuous culture conditions, all strains tested grow in a chemically defined medium (cdm). the purposes of this study were to determine whether resting cells of f. nucleatum could attack unsubstituted peptides and whether growing cells could utilize a peptide fraction prepared from a commercial peptone. resting cells cleaved all 19 pe ...19921494454
golden hamster embryonic genome activation occurs at the two-cell stage: correlation with major developmental changes.the earliest time of onset of embryonic genome activation in golden hamsters was investigated. the inhibition of transcription by alpha-amanitin (11 micrograms/ml) in cultured embryos resulted in a total arrest of development of early 2-cell embryos (26 hr post-egg activation); under similar conditions, immediate cleavage divisions of 1-, late 2-, 4-, and 8-cell embryos were not affected. electrophoretic analysis of [35s]methionine-labeled embryonic proteins showed that alpha-amanitin treatment ...19921497872
primary culture of human fallopian tube epithelial cells and co-culture of early mouse pre-embryos.we have established a monolayer culture system for human fallopian tube epithelial cells. the cells were isolated from tubes using collagenase digestion, and were cultured in ham's f-10 supplemented with 15% fetal calf serum. the epithelial cells derived from culture were characterized using immunocytochemical staining and electron microscopy. these cells were stained with antikeratin and anti-epithelial membrane antigen, but showed no staining after treatment with antivimentin. electron microsc ...19921497873
[botryomycosis caused by fusobacteria].in the anglo-american literature botrymycosis is described as a chronic cutaneous granulomatous reaction to bacterial infection, containing granules resembling the sulphur granules seen in actinomycosis. the diagnostic and therapeutic aspects are discussed with reference to a recently observed patient. fusobacterium nucleatum was isolated as the bacterial cause of the pathologic disorder.19921506205
ruptured splenic abscess secondary to infectious mononucleosis.a 24-year-old woman with a recent diagnosis of infectious mononucleosis presented with fever and left upper quadrant pain. abdominal computed tomography revealed a splenic abscess that at laparotomy was found to have ruptured. this case illustrates a rare cause of splenic abscess and a rare complication of infectious mononucleosis.19921514735
the effect of cyclosporin and lipopolysaccharide on fibroblasts: implications for cyclosporin-induced gingival overgrowth.drug-induced gingival overgrowth is an adverse side effect associated principally with 3 different types of drugs; specifically the antiepileptic phenytoin, the calcium channel antagonist nifedipine, and the immunosuppressant cyclosporin. the present study has analyzed the effect of cyclosporin and lipopolysaccharide on fibroblasts from 3 different sources: 1) normal healthy human gingiva (nhgf); 2) overgrown gingiva from 2 patients taking cyclosporin (chgf); and 3) human fetal lung (wi-38). fib ...19921527683
the early colonization of gram-negative anaerobic bacteria in edentulous infants.the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ...19921528621
serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin.numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ...19921528630
comparative mitogenicity and polyclonal b cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu-balb/c) and thymic (balb/c) mice.optimal stimulatory doses of purified phenol-water extracted lipopolysaccharides (lps) from 5 selected strains of 3 putative periodontopathogens (fusobacterium nucleatum, prevotella intermedia, and veillonella), 3 strains of 2 nonoral bacterial species (bacteroides fragilis and salmonella enteritidis), and pokeweed mitogen (pwm) induced significantly higher maximum mitogenic responses and polyclonal ig production in cultures of unfractionated spleen cells from nu/nu-balb/c (nude) than from balb/ ...19921528635
a non-lectin-like mechanism by which fusobacterium nucleatum 10953 adheres to and activates human lymphocytes.most (approximately 80%) strains of fusobacterium nucleatum adhere to human erythrocytes in a lectin-like manner that is strongly inhibited by n-acetyl-d-galactosamine (galnac). in this study, we investigated the capacity of f. nucleatum 10953, a strain that is weakly inhibited by galnac, to adhere to and activate human lymphocytes in vitro. experiments using [3h]-labeled bacteria and scanning electron microscopy clearly showed that 10953 adhered to lymphocytes and that adherence was blocked by ...19921528636
isolation and properties of the capnocytophaga ochracea bacteriocin.a bacteriocin from a subgingival plaque isolate of capnocytophaga ochracea was purified and characterized. it was isolated from cell extract and had a molecular weight of 100,000. streptococcus sanguis, streptococcus mitis, streptococcus mutans and propionibacterium acnes were susceptible to the bacteriocin.19921528638
polymorphism in the rd (d6s45) gene.the rd (d6s45) gene in the class iii region of the hla major histocompatibility complex encodes a protein normally containing 24 consecutive basic-acidic dipeptide repeats. we determined the frequency of variations in the number of repeats by use of the polymerase chain reaction. of 107 subjects 7 (3.3%) carried genes encoding 22 or 23 repeats. there was no difference in the frequency of such polymorphisms between normal individuals and those with systemic lupus erythematosus, a disease associat ...19921534063
superoxide dismutase and catalase in an experimental model of multiple organ failure.multiple organ failure (mof) can be induced by sterile intraperitoneal injection of zymosan in the rat. this results in a typical triphasic illness with maximal clinical signs at days 2 and 12. in this model, superoxide production and lipid peroxidation closely correlate with the triphasic clinical illness. in the present experiment we studied the effect of albumin conjugated superoxide dismutase (sod) and catalase (cat) on lipid peroxidation and organ damage in the acute phase (days 1 and 2). l ...19921538604
regulations for implementing the clinical laboratory improvement amendments of 1988: a 1988, congress passed the clinical laboratory improvement amendments (clia), which set standards to improve the quality of clinical laboratory testing in all laboratories in the nation that conduct testing on human specimens for health assessment or for the diagnosis, prevention, or treatment of disease. this report summarizes the final regulations for implementing clia and provides an overview of each section, specifying laboratory standards and requirements.19921538689
thiol and aspartyl proteolytic activities in secretory vesicles of bovine pituitary.thiol and aspartyl proteolytic activities in isolated secretory vesicles of neural (nl) and intermediate (il) lobes of bovine pituitary were characterized with heterologous enkephalin and tachykinin precursor substrates, 35s-(met)-preproenkephalin and 35s-(met)-beta-preprotachykinin. il and nl secretory vesicles contained thiol-dependent proteolytic activity that cleaved the enkephalin precursor with a ph optimum of 4.5; this activity resembled a novel "prohormone thiol protease' previously puri ...19921550554
serial doxycycline and metronidazole in prevention of recurrent periodontitis in high-risk patients.the efficacy of metronidazole and doxycycline in preventing recurrent periodontitis was studied in 23 patients. after treatment in the previous 7 months with either bimonthly scaling and 3 weeks of systemic doxycycline (11 subjects) or scaling and placebo (12 subjects), patients were monitored for recurrent periodontitis and were scaled every 2 months. when either a periodontal abscess or greater than 2 mm loss of gingival attachment was observed, metronidazole was administered (250 mg every 8 h ...19921552469
synergistic degradation of bovine serum albumin by mutans streptococci and other dental plaque bacteria.mutans streptococci (streptococcus mutans and streptococcus sobrinus) exhibited low levels of proteolytic activity against the model protein substrate, fitc-labelled bovine serum albumin, when incubated alone. inclusion of other members of the dental plaque microflora in the assay usually resulted in marked increases in the degree of proteolysis and a high level of synergy. interactions between mutans streptococci and either streptococcus oralis or fusobacterium nucleatum gave rise to the greate ...19921555762
messenger rna sorting in enterocytes. co-localization with encoded proteins.this study describes the intracellular compartmentalization of three different mrnas in the polarized rat fetal enterocyte. they encode proteins that are known to be localized within different regions of the epithelial cell namely (i) the apical, membrane-bound glycoprotein, lactase-phlorizin hydrolase (lactase), (ii) the mitochondrially localized enzyme, carbamoylphosphate synthetase (cps), and (iii) the cytoplasmically localized enzyme, phosphoenolpyruvate carboxykinase (pepck). these mrnas ar ...19921563519
fusobacterium nucleatum subsp. fusiforme subsp. nov. and fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within fusobacterium nucleatum.using a variety of physiological, biochemical, and molecular systematic analyses, we have shown previously that there are four groups within the species fusobacterium nucleatum. two of these groups of strains correspond to the recently proposed taxa f. nucleatum subsp. nucleatum and f. nucleatum subsp. polymorphum. in this paper we show that the two remaining groups are distinct and formally propose that they should be recognized as f. nucleatum subsp. fusiforme (type strain, nctc 11326) and f. ...19921581188
polymerase chain reaction analysis of renin in rat aortic smooth muscle.controversy exists whether vascular smooth muscle cells in vivo synthesize renin, thereby providing a critical component of the hypothesized vascular renin-angiotensin system. to examine this question, we enzymatically isolated and pooled the medial layer of thoracic aortas from sprague-dawley rats that were either untreated or enalapril treated for 3 days, isolated messenger rna (mrna), and performed northern blot analysis with rat complementary dna (cdna) probes for renin, cathepsin d, and cat ...19921592470
polymerase chain reaction analysis of renin in rat aortic smooth muscle.controversy exists whether vascular smooth muscle cells in vivo synthesize renin, thereby providing a critical component of the hypothesized vascular renin-angiotensin system. to examine this question, we enzymatically isolated and pooled the medial layer of thoracic aortas from sprague-dawley rats that were either untreated or enalapril treated for 3 days, isolated messenger rna (mrna), and performed northern blot analysis with rat complementary dna (cdna) probes for renin, cathepsin d, and cat ...19921592470
transforming growth factor beta induces myoblast differentiation in the presence of mitogens.transforming growth factor beta 1 (tgf-beta 1) added to l6e9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the g1 phase of the cell cycle. this growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. these results suggest that tgf-beta 1 may act as ...19921594628
fusobacterium nucleatum bacteraemia with multiple liver abscesses in an hiv-i antibody positive man with igg2 deficiency.liver abscess formation due to fusobacterium nucleatum is rare. we describe an hiv-i antibody positive man, with normal surrogate markers of cell-mediated immunity, who presented with f. nucleatum bacteraemia and liver abscess formation. he was found to have igg2 subclass deficiency. this case illustrates the clinical importance of altered b-cell function in patients who are at an early stage of hiv disease.19921602152
[polymorphonuclear leukocyte-mediated effects on human oral keratinocytes by periodontopathic bacterial extracts].in order to investigate the polymorphonuclear leukocyte-mediated oral keratinocyte injury, the author established a method to culture keratinocytes (scc9 and gingival keratinocytes) and subjected to the cell detachment assay by polymorphonuclear leukocytes and/or bacterial ultrasonic extracts from actinobacillus actinomycetemcomitans y4, bacteroides forsythus omz 408, fusobacterium nucleatum atcc 10953, capnocytophaga sputigena 4 and eikenella corrodens 1073. the bacterial extracts alone caused ...19921607829
genetically induced inner ear degeneration. a structural and functional study.the jerker mouse mutant has an autosomal recessive mutation, which in homozygotes results in early postnatal degeneration of the sensory epithelia in both the cochlea and vestibulum. an age-related consecutive functional and morphological analysis of the cochlea has been performed in homozygote (je/je) and heterozygote (je/+) jerker mice. cochlear function was determined with auditory brainstem response (abr) technique. heterozygotes were regularly tested to a final age of 12 months. with increa ...19921636414
quantitative analysis of the epstein-barr virus-inducing properties of short-chain fatty acids present in the culture fluids of oral bacteria.the culture fluids of various anaerobic bacteria induced the synthesis of early antigens (ea) in epstein-barr virus (ebv) carrying lymphoblastoid cells. the culture fluids of corynebacterium butyricum and fusobacterium nucleatum were the effective inducer on ea. the inducing activity was, to some extent, dependent on their n-butyric acid content, but appeared to be regulated by yet unidentified materials.19911652240
subgingival microflora of advanced periodontitis in the dominican republic.a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ...19911658290
relation of baseline microbial parameters to future periodontal attachment loss.the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...19911661304
systemic antibody responses to oral microorganisms in the cynomolgus monkey: development of methodology and longitudinal responses during ligature-induced disease.systemic antibody responses to oral microorganisms were studied during ligature-induced periodontal disease in a non-human primate (nhp) model. methodology was developed using elisa techniques to assess total igg and igm levels in the serum from the nhp. in addition, an elisa was developed utilizing affinity-purified anti-human isotype reagents to detect nhp serum antibody responses to porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia and fusobacterium nucleat ...19911665573
phenotypic analysis of b-cells extracted from human periodontal disease tissue.b-cells extracted from periodontal disease tissue were analyzed for the presence of activation markers using a range of monoclonal antibodies. in adult periodontitis (ap), 6% of b-cells expressed the il-2 receptor (cd25) compared with 1-2% in peripheral blood and healthy or marginal gingivitis (h/mg) gingival b-cells. there was also an increase in the mean percentage of igd-positive b-cells and a decrease in cd21 and cd22 expression. in both ap and h/mg lesions, 20-22% of the b-cells expressed c ...19911668249
[severe infections due to streptococcus milleri in children].streptococcus milleri (s. milleri) is found in healthy individuals in the mouth, nasopharynx, throat, vagina and in feces, and has been reported to be isolated from several infectious diseases in man, particularly from abscess in various parts of the body. we report two cases of severe infections due to s. milleri isolated from subdural abscess and pleural empyema. [case 1] 13 year old boy who had been healthy until he was noticed to have meningeal signs and was diagnosed as left subdural absces ...19911680938
measurement of the human immune response to meningococcal lipooligosaccharide antigens by using serum to inhibit monoclonal antibody binding to purified lipooligosaccharide.we developed a human inhibition monoclonal enzyme-linked immunosorbent assay (himelisa) to investigate the human immune response to the lipooligosaccharides (los) of neisseria meningitidis. monoclonal antibodies (mab) were used to define seven epitopes on four los molecules of a meningococcal strain (126e) previously shown to express immunogenic los epitopes. the assay could distinguish epitope-specific antibody within whole sera. neither the specificity nor the amount of the antibody measured b ...19901694820
the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ...19901696949
permeability alteration of the sarcolemmal membrane, particularly at the site of macrophage contact, in experimental chronic trypanosoma cruzi myocarditis in mice.sarcolemmal membrane permeability characteristics have been investigated, particularly at the site of macrophage contact, in experimental chronic trypanosoma cruzi myocarditis in balb/c mice, employing ruthenium red (rr) as an electron tracer. the ultrastructural features of the myocardium from infected animals were similar to those previously described. briefly, focal myocarditis was detected, with areas of myocytolytic necrosis, atrophic myofibres, an inflammatory response composed of mononucl ...19901698094
[studies on defense effects of recombinant human granulocyte colony-stimulating factor (g-csf) to infections. ii. priming effect for superoxide production by human neutrophil].in present study, we have investigated superoxide (o2-) production from human neutrophils by recombinant human granulocyte colony-stimulating factor (g-csf) using the microtiter plate for the purpose of being close to the inflammatory site. g-csf by itself did not induce the release of o2- in human neutrophil on either fetal bovine serum (fbs)-coated plate or plate uncoated with fbs, even if neutrophils were exposed for maximum 3 hr. however, the optimal concentration of g-csf (50 ng/ml) was abl ...19901698209
conduction velocity distribution of afferent fibers innervating the rat urinary bladder.the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ...19901698508
intrageneric relationships of members of the genus fusobacterium as determined by reverse transcriptase sequencing of small-subunit rrna.the phylogenetic interrelationships of 14 members of the genus fusobacterium were investigated by performing a comparative analysis of the 16s rrna sequences of these organisms. the sequence data revealed considerable intrageneric heterogeneity. the four species fusobacterium nucleatum (including f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, "f. nucleatum subsp. fusiforme," and "f. nucleatum subsp. animalis"), fusobacterium alocis, fusobacterium periodonticum, and fusobacterium ...19911715737
the association of occult amniotic fluid infection with gestational age and neonatal outcome among women in preterm evaluate the relationships between gestational age, neonatal outcome, and amniotic fluid (af) bacteria, we obtained af from women with intact membranes in idiopathic preterm labor. positive cultures were obtained from 20 (19%) of 105 women. the frequency of positive cultures was inversely related to gestational age: 23-26 weeks, nine of 20; 27-30 weeks, four of 24; and 31-34 weeks, seven of 61 (chi2 for trend, p less than .001). fusobacterium nucleatum, bacteroides ureolyticus, and ureaplasma ...19921738513
fusobacterium nucleatum pyomyositis. 19921738725
osteomyelitis of the pelvis due to fusobacterium nucleatum.anaerobic pelvic osteomyelitis occurs rarely in children. a case of fusobacterium nucleatum osteomyelitis of the right ilium [correction of ileum] in a child without apparent risk factors is described. this case emphasizes the value of diagnostic aspirates. therapy with a combination of intravenous and oral clindamycin led to successful resolution of the infection.19911747843
the microflora of periodontal sites showing active destructive progression.20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ...19911752997
fluorescence immunoassay for detecting periodontal bacterial pathogens in plaque.a particle concentration fluorescence immunoassay has been modified into a bacterial concentration fluorescence immunoassay (bcfia) to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilizes fluorescently tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative plaque bacteria. microorganisms closely associated with periodontal disease that can be identified in plaque with the bcfia include porphyromonas gingivalis, bacter ...19911761686
analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development.using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ...19911769342
isolation and identification of bacteria from subgingival plaque with low susceptibility to minocycline.twenty subgingival plaque samples from patients with chronic periodontitis were screened for the presence of three periodontal 'pathogens'--porphyromonas gingivalis, fusobacterium nucleatum and eikenella corrodens. nineteen of the samples were found to contain at least one of the three organisms and six samples contained all three. the subgingival plaque samples were cultured in the presence of various concentrations of minocycline, and those organisms which appeared to be least susceptible to t ...19911769945
differential protective activities of site specific lipoxygenase inhibitors in endotoxic shock and production of tumor necrosis factor.lipoxygenase inhibitors have been shown to exert beneficial effects in experimental models of endotoxin shock. in the present study it was found that lipoxygenase inhibitors prevented lps, but not tumor necrosis factor alpha (tnf alpha)-evoked leukopenia in mice. these inhibitors protected against endotoxin lethality but not against tnf alpha induced lethality. when the protective potency of the specific 5-lipoxygenase inhibitors (mk 886, cgs 81585) was tested in endotoxin-induced leukopenia and ...19911783470
[subgingival microflora in children of early childhood, school age and circumpuberty. the proportion and frequency of gram-negative bacteria in periodontally healthy and gingivitis groups].the present study characterized the microbial profiles of the gingival sulci in children. subgingival samples from 36 gingivitis lesions of 18 patients and 36 sites in 18 healthy persons were examined. the tested individuals were divided into three stages according to physiological maturation i.e. early childhood, school age, circumpuberty. puberty was confirmed through examination of wrist radiographs. using continuous anaerobic techniques, samples were dispersed, diluted and then inoculated on ...19911784868
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
bacteria in the apical 5 mm of infected root canals.ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...19911809801
[the dynamic modification of aseptic inflammation with sodium oxybutyrate].the oxybuturate sodium influence on the vessel and cells dynamics in the source of aseptic inflammation was studied. it was shown that oxybuturate sodium injection (100 mg/kg) before 30 min and after 2 and 4 hours of inflammation onset leads to suppression of microvessel reaction, reduction of macrophagic infiltrate density, decrease of macrophagic phase duration and the earliest perfection of inflammation in the whole.19911810478
adherence to experimental pellicle of rough-type lipopolysaccharides from subgingival plaque bacteria.the adherence of lipopolysaccharides (lpss) from periodontal disease-associated bacteria to saliva-coated hydroxyapatite (s-ha) and serum-coated ha beads was examined by chromogenic limulus activity (toxicolor test). phenol-water extracted lps preparations from bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, eikenella corrodens, and rough-type lps from escherichia coli adhered to s-ha and serum-coated beads and agglutinated human erythrocytes. the adhered ...19911812466
aspects of the growth and metabolism of fusobacterium nucleatum atcc 10953 in continuous culture.fusobacterium nucleatum atcc 10953, a type strain of one of the newly proposed subspecies of this group of organisms, was grown anaerobically in continuous culture in a chemically defined medium. its response to conditions of varying ph, nutritional environment, and imposed growth rate were then examined. the organism failed to grow at ph 7.8 but grew at ph 5.8, although the cell yield was greatly reduced. at ph 6.8 the cell yield was halved and less than 50% of available glucose was consumed. t ...19911812468
antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens.nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ...19911812907
[effect of the type of swab on the isolation of chlamydia trachomatis].the success in the isolation of c. trachomatis is dependent on several factors. one of them is the type of swab used for collection the specimens. as some types of swabs, such as calcium alginate and aluminum, cotton and wood have recently been shown to decrease the isolation rate, different types of swabs were prepared and tested in our laboratory. commercial aluminum, type 5056 aluminum and stainless steel were used with the same type of cotton. commercial swabs of cotton and wood were tested ...19911815280
formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria.during the course of our studies on the metabolism of iridoid glycosides by human intestinal bacteria, we found that geniposide (1) and gardenoside (4) were transformed to new nitrogen-containing compounds, genipinine (3) and gardenine (6), respectively, along with the known aglycones. although the amounts of new metabolites were somewhat lower than those of the aglycones, they were quantitatively analyzed by means of liquid chromatography/mass spectrometry (lc/ms). of 25 strains of human intest ...19911818345
a heat stable hemagglutinating activity of eikenella corrodens.strains of eikenella corrodens and fusobacterium nucleatum were shown to possess lectinlike attachment factors. the present investigators found that e. corrodens has a heat stable factor which agglutinates sheep, mouse, and human erythrocytes. the activity is not abolished by additions of galactose or lactose. lipopolysaccharide preparations from e. corrodens strains possessed strong hemagglutinating activity. lpss of f. nucleatum did not agglutinate any erythrocytes tested. sds-page patterns st ...19911819444
adhesive properties of strains of fusobacterium nucleatum of the subspecies nucleatum, vincentii and polymorphum.this study surveyed some adhesive properties of strains of fusobacterium nucleatum representative of the 3 recently defined groups or subspecies that could relate to their colonization and virulence. with one exception, f. nucleatum strains agglutinated sheep erythrocytes, but the quantity of bacteria required and the sensitivity of the hemagglutination reactions to inhibition by 0.05 m galactose or arginine varied between strains, and did not exhibit clear-cut correlations with subspecies. neur ...19911820561
comparison of the amino acid uptake profile of reference and clinical isolates of fusobacterium nucleatum subspecies.human isolates of fusobacterium nucleatum subspecies appear to colonize different niches in the oral cavity, which may be reflected in their nutritional properties. consequently the utilization of nitrogenous substrates, their sources of energy (supplied here as amino acids) were compared between the 3 subspecies using the reference strain and fresh clinical isolates of each subspecies. all strains incorporated mainly acidic and basic amino acids but significant differences occurred between subs ...19911820562
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