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[details of a denounced physician (e.n.t.) suspected of bodily injury, and the following expert judgement (author's transl)].an otologist was denounced and the patient's case history was confiscated because after tonsillectomy an absecess and a thrombosis of the vena jugularis interna had developed. the procedure and the medical aspect how at first being judged by the lawyer and the public prosecutor are described. on the base of the given medical opinion the court quashed proceedings. because of the growing number of denouncements this casuistic paper ends with four guiding principles to protect against unjustified m ...2002135139
[morphological differentiation of neuroblastoma by 1 methyl cyclohexane carboxylic acid (cca) and some c1 derivatives].we describe the induction of neuroblastoma morphological differentiation by 1 methyl cyclohexane carboxylic acid (cca) and by some c1 derivatives of cca. this induction proceeds in a medium which allows, in the absence of inducer, a normal growth of neuroblastoma cells and contains 7.5% fetal calf serum. in order to establish a clear-dose response relationship and to compare the relative potencies of different drugs, the morphological changes were assessed by examining "long neurite-bearing cell ...2002120786
[pharmacological influence of valproate on phenobarbitone and phenytoin serum concentrations in epileptic patients (author's transl)]. 2002119918
benefit-cost and cost-effectiveness analysis: theory and application.benefit-cost analysis and cost-effectivensss analysis are terms used with increasing frequency by health planners and those concerned with review and evaluation of specific programs. the economic bases for these formal techniques are however often obscured by adaptations of convenience or misapplications of the concepts by biological scientists (and others). this paper reviews briefly the theory of benefit-cost analysis and its potential as a tool in choosing programs of optimum size, of maximum ...2002118068
cricopharyngeal sphincterotomy and bilateral division of the chorda tympani in bulbar palsy. 2002128757
the surgical management of low back and sciatic syndrome in disc disease or injury : results of a joint neurosurgical and orthopaedic project. 2002127575
letter: special classes. 2002127593
changes in the t-lymphocyte subpopulation (cd+/cd8+) in the blood of patients following kidney transplant and during the acute rejection episode.a study was made of changes in the ratio of cd4+/cd8+ in patients who had received cadaverous kidneys and who were under treatment with a combination of cyclosporin a + azathioprine + cor ticosteroids. the results showed that one month after the transparent there was a significant drop (p less than 0.05) in the immunoregulation index compared with the pretransplantation values. throughout the whole period a correlation (r = -0.65, p less than 0.05) was found between the cd4+/cd8+ ratio and serum ...20021906396
[current status of studies of immune complex]. 2002127852
medicaid stays are short. 2003150521
[the surgical treatment of hiatal hernia and gastroesophageal reflux]. 2003326645
psycho-social aspects of recovery from coronary heart disease: a review. 2003329420
severe orthopedic disability in childhood: solutions provided by rehabilitation engineering. 2003149287
enhancement of lymphocyte and macrophage function in vitro by lithium chloride. 2003149624
isolated azygos continuation of the inferior vena cava. 2003262529
[hygienic evaluation of the effectiveness of measures for the sanitary improvement of the atmosphere in the region of a metallurgical plant]. 2003153874
radiosurgical treatment of cerebral metastases. method, indications and results.stereotactically guided single-dose irradiation of brain metastases with low radiosensitivity has been performed in a series of 30 patients. clinical symptoms improved in 18 of 27 evaluated patients and remained stable in another 7. tumour regression was achieved in 13 of 22 patients, and arrest of tumour growth in 7 other patients. the treatment was usually well tolerated. the incidence of side effects was low. thus, radiosurgery seems to be a valuable alternative to microsurgical extirpation o ...20031808657
[computers applied to dietetics]. 2003262700
presynaptic receptor systems on cardiac sympathetic nerves. 2003222983
femtosecond biology. 20031326353
using hospital staff to provide aftercare: kudos and criticism. 2003193775
aging and the visceral musculature of the adult fruitfly: an ultrastructural investigation. 2003403646
[diagnostik significance of cell membrane markers in childhood all (author's transl)].acute lymphoblastic leukemia (all) in childhood is not a homogeneous disease. by membrane phenotyping 6 groups of all in 66 children could be differentiated: group i (38%) common all (anti-call+. group ii (32%) common all with t cell marker in addition (anti-call+, anti-t+). group iii (14%) t. cell all with e-rosette marker (anti t+, e+). group iv (9%) t cell all without e-rosette marker (anti t+, e-). group v (6%) undifferentiated all (anti-call-, anti-t). group vi (1%) b cell typ (smig+). 19 o ...2003309025
[in the memory of john c. belisario 1900-1976]. 2003328453
cushing's syndrome or obesity. bilateral adrenal hyperplasia in a boy 10 years of age.a rare case of the cushing syndrome, due to bilateral adrenal hyperplasia is described. because of generalized obesity, normal height, normal bone age and family history of obesity, a boy 10 years of age had at first been misdiagnosed as simple obesity. a reduction in height velocity, advancement of bone age and development of precocious pseudopuberty led to a more detailed biochemical investigation and to the correct diagnosis. the clinical picture can be misleading in the differential diagnosi ...2003179822
[behavior of plasma catecholamines in relation to pressure changes induced with hemodialysis or ultrafiltration]. 2003262276
[combined treatment of rheumatological patients at a health resort]. 2003154775
[eosinophilic leukosis]. 2003290268
[electrono-optical analysis of dental enamel with incipient pathological changes]. 2003155841
richter's hernia at site of insertion of laparoscope. 2003145269
laparoscopic removal of the distal catheter of ventriculoperitoneal shunt (ames valve). 2003161536
a new device to support handicapped patients during venography or myelography.the authors describe a seating device which holds the patient in position when the x-ray table is tilted and permits non-weight-bearing venograms to be obtained in handicapped as well as normal patients. simultaneous bilateral venography reduces the study time considerably. the device is also helpful in lumbar myelography in paraplegics.2003149336
primary inoculation tuberculosis.a case of primary inoculation tuberculosis of the finger is described, in which mantoux test conversion reverted after prompt surgical and medical treatment. the pathology of primary inoculation tuberculosis is reviewed briefly, and the rationale for excision of the early tuberculous lesion is discussed.2003155709
[70th anniversary of the s. p. fedorov leningrad urological society]. 2003152495
thy-1 antigenicity is associated with glycolipids of brain and thymocytes. 2003308963
the rate of diffusion of urea to the c.s.f. in diabetics. 2003262251
[the heart in hypertension. iv. ventricular mass to volume ratio and appropriateness of hypertrophy]. 2003152525
[laparoscopy in the diagnosis of hepatobiliary anomalies]. 2003152516
[introduction of legal precedents in nursing accidents. 11. accidents caused by intramuscular injections]. 2003253811
[angiography and emergency embolisation of digestive haemorrhages (author's transl)]. 2003308945
social security in review. 2003150054
genomics of oral bacteria.advances in bacterial genetics came with the discovery of the genetic code, followed by the development of recombinant dna technologies. now the field is undergoing a new revolution because of investigators' ability to sequence and assemble complete bacterial genomes. over 200 genome projects have been completed or are in progress, and the oral microbiology research community has benefited through projects for oral bacteria and their non-oral-pathogen relatives. this review describes features of ...200312799321
antibacterial activity of medicinal plant extracts against periodontopathic bacteria.this study was performed to evaluate the antibacterial activity of althaea officinalis l. roots, arnica montana l. flowers, calendula officinalis l. flowers, hamamelis virginiana l. leaves, illicium verum hook. fruits and melissa officinalis l. leaves, against anaerobic and facultative aerobic periodontal bacteria: porphyromonas gingivalis, prevotella spp., fusobacterium nucleatum, capnocytophaga gingivalis, veilonella parvula, eikenella corrodens, peptostreptococcus micros and actinomyces odont ...200312820224
relationship between periodontal pocket sulfide levels and subgingival species.many species implicated in the pathogenesis of periodontal disease produce volatile sulfur compounds (vsc). this investigation examined the relationship between levels of sulfide and subgingival bacterial species in the same periodontal pockets.200314761124
bacteria-binding plasma proteins in pellicles formed on hydroxyapatite in vitro and on teeth in vivo.previous studies on dental pellicle formation and bacterial adherence have focussed on saliva and its components. the tooth surface is, however, also exposed to the plasma-derived crevicular fluid. in the present study, (i). plasma proteins in in vitro and in vivo pellicles were examined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page), immunoblotting and image analysis and (ii). the adherence of periodontopathogenic bacteria to experimental plasma and saliva pellicles ...200312823794
an immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions.we immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. the bacteria in the specimens were detected by brown-brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. seventy percent of the examined teeth showed bacteria in ...200312669880
antimicrobial properties of self-etching primer-bonding systems.self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ...200312670069
palmitoleic acid isomer (c16:1delta6) in human skin sebum is effective against gram-positive bacteria.the percent lipid composition of pooled human sebum analyzed by thin-layer chromatography was: ceramides (13%), fatty acid (47%), cholesterol (7%), cholesterol esters (2%), squalene (11%), triglycerides (3%), and wax esters (17%). total sebum lipids (2- 4 mg/ml), sonicated into bacterial culture medium, caused 4- to 5-fold log reduction in growth of gram-positive bacteria, staphylococcus aureus, streptococcus salivarius and the anaerobe fusobacterium nucleatum, but was ineffective against most g ...200312677098
ultrastructural analysis of an infected collagen-coated vascular graft.the incidence of infection following arterial reconstruction using synthetic graft materials varies from less than 1 to 5%. one of three mechanisms is thought to be responsible: 1. intraoperative contamination, 2. extension from adjacent infected or colonized tissue, or 3. hematogenous or lymphogenous seeding. we present ultrastructural data of a patient with a polymicrobial graft infection due to a prostheto-enteric fistula 16 years after reconstruction of an aortobifemoral graft. the polymer s ...200312677763
can toothpaste or a toothbrush with antibacterial tufts prevent toothbrush contamination?periopathogens and cariogenic species survive more than 48 hours on toothbrushes even under dry storage. this study examined a decontamination of toothbrushes by means of the bactericidal effect of different toothpastes or a special coating of the tufts.200312710750
[extrinsic ureteral obstruction secondary to inflammatory gynecologic pathology].to present two cases of extrinsic ureteral obstruction secondary to gynaecological inflammatory diseases, and to perform a bibliographic review.200312731448
fusobacterium nucleatum: a rare cause of bacteremia in neutropenic patients with leukemia and lymphoma.although anaerobic bacteremias are uncommon in oncohematologic patients, nevertheless they have been considered an emergent problem in the last few years. fusobacterium nucleatum is an anaerobic gram-negative bacillus commonly present in the oral cavity and in the respiratory and genito-urinary tracts. over a 10-year period 18 episodes of f. nucleatum bacteremia in patients with hematological malignances (15 leukemias and 3 lymphomas) have been observed in our department of hematology. predispos ...200314616727
expression of mhc class ii, cd70, cd80, cd86 and pro-inflammatory cytokines is differentially regulated in oral epithelial cells following bacterial challenge.oral epithelium may play a regulatory role in local immune responses when interacting with bacteria. the present study was undertaken to investigate the effects of selected bacterial pathogens found in periodontal and endodontic infections on oral epithelial cells. expression of cell surface molecules (major histocompatibility complex (mhc) class ii, cd54, cd70, cd80 and cd86) and secretion of inflammatory cytokines (interleukin (il)-1beta, il-6, and tumor necrosis factor (tnf)-alpha) in respons ...200314622340
the influence of organic load on the antimicrobial activity of different concentrations of naocl and chlorhexidine in vitro.to evaluate bacterial growth after contact with sodium hypochlorite (naocl; 1 and 5%) and chlorhexidine (chx; 0.12, 0.5 and 1%) in vitro with or without the addition of organic material (bovine serum albumin (bsa) 0.5%).200314641423
bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer.the goal of this study is to determine if the smear layer affects the passage of bacteria through or around obturating material as evidenced by penetration of bacteria through and out the canal. specifically, this study focused on determining the effect of the smear layer on the magnitude of bacterial penetration through the apical foramen.200312735922
culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients.renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ...200314662931
actinobacillus actinomycetemcomitans serotype b lipopolysaccharide mediates coaggregation with fusobacterium nucleatum.purified actinobacillus actinomycetemcomitans serotype b lipopolysaccharide (lps) was found to be able to bind fusobacterium nucleatum cells and to inhibit binding of f. nucleatum to a. actinomycetemcomitans serotype b. sugar binding studies showed that the requirements for binding of a. actinomycetemcomitans serotype b lps to the f. nucleatum lectin are the presence of a metal divalent ion, an axial free hydroxyl group at position 4, and free equatorial hydroxyl groups at positions 3 and 6 of d ...200312761156
[early diagnosis of bacterial brain abscesses: interest of diffusion-weighted mri].three cases of bacterial brain abscesses, in immunocompetent patients, are reported. in all these cases, the diffusion-weighted magnetic resonance (mri) with apparent diffusion coefficient (adc) map has permitted an early diagnosis and a rapid treatment. this emergency mri showed in the three cases a low signal on ti-weighted images, a high signal on t2-weighted and echo-planar images, and a decrease of adc (0.36- 0.49 x 10(-3) mm2/s). so, this new mri technique provides an available and rapid e ...200312773871
additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial pathogens.it is an established procedure to use calcium hydroxide [ca(oh)2] as a therapeutic component to achieve sterilization of infected root canals. unfortunately, some bacterial species are relatively resistant to ca(oh)2 and are therefore associated with treatment failures. the objective of this study was to identify combinations of substances that improve antimicrobial activity. ca(oh)2 was used as a suspension or as a component of gutta-percha points. efficacy of killing was compared for (a) ca(oh ...200312775007
characteristics of brain abscess with isolation of anaerobic bacteria.in view of its localization, brain abscess (ba) usually requires medical and surgical care. a broad spectrum of bacteria is involved. recent reports stress the increasing frequency of anaerobes, but their impact has not been well evaluated. a retrospective review was conducted of all episodes of documented ba admitted in a tertiary-care hospital over a 10 y period. ba due to anaerobic bacteria (group a) were compared with other cases (group b) to determine the frequency and eventual characterist ...200312875518
positive and negative bacterial associations involving dialister pneumosintes in primary endodontic infections.dialister pneumosintes is an anaerobic gram-negative rod that has been recently implicated as a candidate endodontic pathogen. in this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving d. pneumosintes. dna was extracted from the samples, and the presence of d. pneumosintes and 16 other bacterial species was determined by means of species-specific ...200312877258
mass transport of macromolecules within an in vitro model of supragingival plaque.the aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. polyspecies biofilms containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, streptococcus sobrinus, veillonella dispar, and candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. subsequent examinatio ...200312620862
the reduction of fusobacterium nucleatum in mice is irrelevant to the nitric oxide induced by inos.previously we reported that mice infected recurrently with live fusobacterim nucleatum (fn) synthesize a significant amount of no between 12 hr and 24 hr after the fn injection. we now investigated whether the no has the capability of killing fn, a gram-negative rod periodontal pathogen. the mice were divided into three groups: treated with live bacteria (lb), treated with heat-killed bacteria (hkb) and untreated: normal (n). the fn reduction, no production and cell number after fn injection wer ...200312636250
serum antibodies of periodontitis patients compared to the lipopolysaccharides of porphyromonas gingivalis and fusobacterium nucleatum.serum antibody titers against the lipopolysaccharides (lpss) of porphyromonas gingivalis and fusobacterium nucleatum were compared between 9 periodontitis patients and 24 healthy persons. the igg titers against the lpss of p. gingivalis atcc 33277(t) and w50 were clearly higher in the patients than in the healthy persons. however, igm titers against the lpss of p. gingivalis strains were relatively low, and no significant difference was observed between the patients and healthy persons. on the o ...200312636253
coaggregation of streptococcus salivarius with periodontopathogens: evidence for involvement of fimbriae in the interaction with prevotella intermedia.streptococcus salivarius is divided into two serological subgroups that carry either fibrils or fimbriae. although fimbriae have been observed on up to 50% of s. salivarius strains in the human oral cavity, no function has yet been assigned to them. to determine whether s. salivarius fimbriae have a role in adhesion, we examined the ability of s. salivarius to coaggregate with selected microorganisms involved in periodontal diseases. our results show that s. salivarius coaggregated with fusobact ...200312930529
multiple brain abscesses caused by fusobacterium nucleatum treated conservatively.multiple brain abscesses are serious neurological problems with high mortality and disabling morbidity. the frequency is rising as a result of aids and the increasing number of immunocompromised patients.200312945954
acid-neutralizing activity during amino acid fermentation by porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum.acid-neutralizing activity during amino acid fermentation by washed cells of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum was studied. when the washed cells of these strains were anaerobically incubated in the presence of aspartylaspartic acid or glutamylglutamic acid for p. gingivalis, aspartic acid for p. intermedia and glutamic acid for f. nucleatum at an initial ph of 5.0 or 5.5, the ph of the incubation mixtures rose toward neutral. f. nucleatum had the highes ...200312654101
bactericidal activities of methoxyfluoroquinolones gatifloxacin and moxifloxacin against aerobic and anaerobic respiratory pathogens in serum.gatifloxacin (bristol-myers squibb) and moxifloxacin (bayer) are new methoxyfluoroquinolones with broad-spectrum activity against aerobic and anaerobic pathogens of the respiratory tract. in this investigation, we analyzed the bactericidal activity in serum over time of these antimicrobials against three aerobic (streptococcus pneumoniae, haemophilus influenzae, and staphylococcus aureus) and four anaerobic (peptostreptococcus micros, peptostreptococcus magnus, fusobacterium nucleatum, and prevo ...200312654663
the microflora recovered from the outer-surfaces of the frialit-2 implanto-prosthetic connector.the aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. in 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 frialit-2 implants. the functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. after r ...200312562362
a new method for rapid screening of bacterial species- or subspecies-specific dna probes.a simple assay for the rapid screening of bacterial species- or subspecies-specific dna probes for the random cloning method is presented, involving the use of genomic dnas as probes and recombinant plasmid dnas containing genomic dna digested with hindiii as targets. the optimal amount of target dnas and the concentration of digoxigenin-labeled genomic dna probes were 20 ng and 100 ng ml(-1) (or 10 ng and 200 ng ml(-1)), respectively. the method was applied to the development of fusobacterium n ...200312594033
antibacterial effects of resinous retrograde root filling materials.the effect of three resinous retrograde root filling materials on the growth of four obligate anaerobic bacteria associated with endodontic infections was determined by using the agar diffusion inhibitory test. samples of the following resinous materials (resin-modified, glass-ionomer cement, fuji ii lc; compomer, dyract; and composite resin, spectrum) were incubated for 1 week with the following anaerobic bacteria: fusobacterium nucleatum, porphyromonas gingivalis, p. endodontalis, and prevotel ...200312597711
subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult sudanese population using miswak or toothbrush regularly.little information is available on the effect of miswak use on gingival microbiota. we assessed levels of 28 oral bacteria in subgingival plaque of adult sudanese miswak (n = 38) and toothbrush users (n = 36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. a pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. whole genomic dna p ...200312790510
genome analysis of f. nucleatum sub spp vincentii and its comparison with the genome of f. nucleatum atcc 25586.we present the draft genome sequence and its analysis for fusobacterium nucleatum sub spp. vincentii (fnv), and compare that genome with f. nucleatum atcc 25586 (fn). a total of 441 fnv open reading frames (orfs) with no orthologs in fn have been identified. of these, 118 orfs have no known function and are unique to fnv, whereas 323 orfs have functional orthologs in other organisms. in addition to the excretion of butyrate, h2s and ammonia-like fn, fnv has the additional capability to excrete l ...200312799352
antimicrobial activity of ca(oh)2 dental cements: an in vitro study.the effect of five commercial calcium hydroxide root canal medications (xr-spad, calcicur, hy-cal, root-cal, hypo-cal) and two calcium hydroxide mixtures (with water or glycerin) on the growth of (a) three anaerobic bacteria associated with endodontic infections; and (b) saliva bacterial strains was determined using the agar diffusion inhibitory test. statistically significant zones of bacterial growth inhibition for all the bacteria tested were observed. calcium hydroxide mixed with glycerin sh ...200312540221
changes in growth and polyglucose synthesis in response to fructose metabolism by fusobacterium nucleatum grown in continuous culture.fusobacterium nucleatum, grown in a chemically defined medium at micro(rel) = 0.5, produced greater cell yields and undetectable levels of intracellular polyglucose (ip) when fructose was substituted for glucose. the utilisation and metabolism of fructose by growing cells was studied and the effect of the energy-yielding amino acids, glutamate, serine, histidine and lysine on cell yield, ip synthesis and acidic end-products was investigated. when f. nucleatum was grown on elevated amino acid lev ...200312823803
lethal photosensitization of periodontal pathogens by a red-filtered xenon lamp in vitro.the ability of helium-neon (he-ne) laser irradiation of a photosensitizer to induce localized phototoxic effects that kill periodontal pathogens is well documented and is termed photodynamic therapy (pdt).200312828662
effect of smoking and periodontal treatment on the subgingival microflora.the effect of smoking on the prevalence of periodontal pathogens after periodontal treatment is still not clear. some studies found no effect of the smoking status on the prevalence of periodontal pathogens after therapy, whereas others did. the aim of this retrospective study was to investigate the influence of smoking on the treatment of periodontitis and the composition of the subgingival microflora.200312834497
unusual presentation of lemierre's syndrome due to fusobacterium nucleatum.we report a case of lemierre's syndrome due to fusobacterium nucleatum in a previously healthy 19-year-old male. this is the first case report of lemierre's syndrome due to thrombophlebitis of the external jugular vein. the patient had a rapid clinical response to anticoagulation and antibiotics, as supported by anecdotal evidence.200312843117
antibiotic resistance of pathogenic bacteria from odontogenic infections in taiwan.the purpose of this study was to evaluate the susceptibility of facultative and anaerobic odontogenic infectious flora to various antibiotics. we assessed 178 bacterial strains isolated from 74 patients with odontogenic infections. the e-test was used to determine susceptibility. the microbial flora was predominantly facultative gram-positive organisms and anaerobic gram-negative bacilli. the results of antimicrobial susceptibility test showed that ampicillin resistance was found with a very hig ...200312886961
antimicrobial constituents from goldenseal (the rhizomes of hydrastis canadensis) against selected oral pathogens.two new c-methyl flavonoids, 6,8-di- c-methylluteolin 7-methyl ether (1) and 6- c-methylluteolin 7-methyl ether (2), were isolated from a commercially available sample of the roots of hydrastis canadensis, along with seven known compounds, berberine (3), beta-hydrastine (4), canadine (5), canadaline (6), isocorypalmine (7), canadinic acid (8), and beta-sitosterol 3- o-beta- d-glucoside (9). the structures of the new compounds 1 and 2 were elucidated on the basis of their spectral data including ...200312898417
effects of a chlorhexidine gluconate-containing mouthwash on the vitality and antimicrobial susceptibility of in vitro oral bacterial ecosystems.oral bacterial microcosms, established using saliva inocula from three individuals, were maintained under a feast-famine regime within constant-depth film fermenters. steady-state communities were exposed four times daily, postfeeding, to a chlorhexidine (chx) gluconate-containing mouthwash (chxm) diluted to 0.06% (wt/vol) antimicrobial content. the microcosms were characterized by heterotrophic plate counts and pcr-denaturing gradient gel electrophoresis (dgge). chxm caused significant decrease ...200312902270
effect of porphyromonas gingivalis vesicles on coaggregation of staphylococcus aureus to oral microorganisms.vesicles from the outer membrane of porphyromonas gingivalis have the ability to aggregate a wide range of streptococcus spp., fusobacterium nucleatum, actinomyces naeslundii, and actinomyces viscosus. we found that in the presence of p. gingivalis vesicles, staphylococcus aureus coaggregated with streptococcus spp., and the mycelium-type candida albicans, but not the yeast type. autoaggregation of s. aureus in the presence of p. gingivalis vesicles is inhibited by l-arginine, l-lysine, and l-cy ...200314756532
intracellular production and extracellular release of oxygen radicals by pmns and oxidative stress on pmns during phagocytosis of periodontopathic bacteria.in this study we investigated intracellular and extracellular oxygen radical production by polymorphonuclear leukocytes (pmns) during the phagocytosis of periodontopathic bacteria. in in vitro assays, bacteria of the species porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum were phagocytosed at 37 degrees c for 4 h by purified peripheral human pmns from healthy subjects (n = 6). superoxide production during phagocytosis was determined by flow cytometry a ...200314505184
[ribotyping fusobacterium nucleatum isolates from healthy and diseased periodontium].fusobacterium nucleatum is one of the most frequently cultured bacteria from subgingival plaque. it can be isolated from active periodontal lesions as well as from healthy sites. currently, f. nucleatum has been divided into five subspecies. an early hypothesis suggested an association between certain subspecies and periodontal disease or health. on the other hand, a broad heterogeneity between f. nucleatum isolates has been suggested, so that the subspeciation scheme and therefore the proposed ...200314558378
intracellular calcium in signaling human beta-defensin-2 expression in oral epithelial cells.expression of human beta-defensins is correlated with differentiation in the oral epithelium, consistent with their function as part of the epithelial antimicrobial barrier. because calcium is a known regulator of epithelial differentiation, we tested the hypothesis that calcium concentration mediates beta-defensin expression. gingival epithelial cells were cultured in medium containing low calcium concentration (0.03 mm), then either changed to high extracellular calcium concentrations or stimu ...200314578498
clinical presentation and bacteriologic analysis of infected human bites in patients presenting to emergency departments.previous studies of infected human bites have been limited by small numbers of patients and suboptimal microbiologic methodology. we conducted a multicenter prospective study of 50 patients with infected human bites. seventy percent of the patients and assailants were young adult men. fifty-six percent of injuries were clenched-fist injuries and 44% were occlusional bites. most injuries were to the hands. fifty-four percent of patients were hospitalized. the median number of isolates per wound c ...200314614671
antagonistic interactions among fusobacterium nucleatum and prevotella intermedia with oral lactobacilli.microbiota residing in oral ecosystems show great complexity because of the mutual interdependence of certain microorganisms that might influence their presence or absence at a given site. the aim of this work was to evaluate possible antagonistic interactions among fusobacterium nucleatum and prevotella intermedia, isolated from periodontal pockets, and lactobacillus casei, l. salivarius, l. rhamnosus and l. plantarum isolated from saliva. different tests were carried out using each strain as a ...200314643404
emergence of penicillin resistance among fusobacterium nucleatum populations of commensal oral flora during early childhood.penicillin resistance due to beta-lactamase production is surprisingly common among oral bacteria in childhood. fusobacterium nucleatum, a gram-negative anaerobic bacillus, is a member of the developing oral commensal flora. as part of the investigation on the emergence of oral bacterial resistance, the aim of the present study was to examine longitudinally the penicillin resistance among salivary f. nucleatum populations as related to age, day care attendance and sibling history, and exposure t ...200312493794
antimicrobial activity of magainin analogues against anaerobic oral pathogens.magainins are a family of potent antimicrobial cationic peptides that possess antimicrobial activity against a wide range of target organisms. in this study, the antimicrobial activity of synthetic magainin-mimetic compounds msi-751 and msi-774 was investigated against the periodontal pathogens porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, eikenella corrodens, prevotella loescheii and prevotella intermedia. p. gingivalis was more susceptible to msi-751 ...200312507842
the effects of a new mouthrinse containing chlorhexidine, cetylpyridinium chloride and zinc lactate on the microflora of oral halitosis patients: a dual-centre, double-blind placebo-controlled study.this study evaluated the microbial effects of a newly formulated mouthwash (halita) on oral halitosis patients.200312716335
plasmid profile in oral fusobacterium nucleatum from humans and cebus apella monkeys.fusobacterium nucleatum is a strict anaerobe and is indigenous of the human oral cavity. this organism is commonly recovered from different monomicrobial and mixed infections in humans and animals. in this study, the plasmid profile, the plasmid stability and the penicillin-resistance association in oral f. nucleatum isolated from periodontal patients, healthy subjects and cebus apella monkeys were evaluated. forty-five f. nucleatum strains from patients, 38 from healthy subjects and seven from ...200312751315
pcr method is essential for detecting mycobacterium tuberculosis in oral cavity samples.tuberculosis is a re-emerging infectious disease, and infection by mycobacterium tuberculosis has been increasing in immunocompromised hosts, including elderly persons. m. tuberculosis-infected persons may receive dental treatment. to evaluate the risk of m. tuberculosis infection in dental clinics, we examined the detection rates of m. tuberculosis in sample of mixed saliva, dental plaque, extracted teeth, caries lesions, and denture plaque by nested polymerase chain reaction (pcr). the detecti ...200312753466
bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy.this study was an attempt to clarify whether the bactericidal effects of photodynamic therapy (pdt) are wavelength or dose-dependent. we also attempted to create an optimised protocol for a light-based bactericidal modality to eliminate periodontal pathogens. cultures of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphromonas gingivalis, prevotella intermedia, and streptococcus sanguis, were exposed to a he-ne laser (632.8 nm) with a 30 mw power output, a 100 mw diode laser a ...200312627274
ovine periodontitis as a potential model for periodontal studies. cross-sectional analysis of clinical, microbiological, and serum immunological parameters.: to investigate infection and host immunity patterns in sheep with naturally occurring "broken-mouth" periodontitis.200312702113
helicobacter pylori and campylobacter rectus share a common antigen.aim: the aim of this study was to investigate the presence of antigens with immunological cross-reactivity in periodontopathogenic bacteria and helicobacter pylori, the pathogen associated with gastritis and peptic ulcers in human. materials and methods/results: among the putative periodontopathogens tested (actinobacillus actinomycetemcomitans, campylobacter rectus, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and treponema denticola), cross-reactive bands were only ...200312654095
fusobacterium nucleatum impairs serum binding to porphyromonas gingivalis biofilm.mouse immune sera obtained by immunization with fusobacterium nucleatum and then porphyromonas gingivalis demonstrated an impaired binding capacity to p. gingivalis-biofilm and lower avidity to p. gingivalis when compared with sera obtained from mice immunized with p. gingivalis alone.200312654097
faropenem, a new oral penem: antibacterial activity against selected anaerobic and fastidious periodontal isolates.the in vitro activity of faropenem, an oral penem, was compared with those of penicillin, co-amoxiclav, cefoxitin, clindamycin, erythromycin and metronidazole against 106 isolates of anaerobic pathogens involved in systemic infections. the organisms tested comprised porphyromonas gingivalis (29), prevotella spp. (eight), prevotella melaninogenica (seven), prevotella intermedia (five), actinomyces spp. (25), fusobacterium nucleatum (14), peptostreptococcus spp. (11), bacteroides ureolyticus (five ...200312615878
note on the oscillations of collapsible tubes. 2003309045
marijuana: pro and con. 2003403763
[experiences with incus autografts and preserved incus homografts in ear surgery (author's transl)].the healing process of free-transplantated incus autografts and preserved homografts in the middle-ear is largely non-irritating. they are suited for sound transmission. under the osteoinductive influence of the non-vital graft, ingrowing vascular connective tissue of the host differentiates itself in osteoblasts, osteoclasts and chondroblasts. the grafts are revitalised in relation to time. rarely a marked resorption of preserved incus homografts was noticed in consequence of an antigen-antibod ...2003142874
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