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efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters.the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition.200111686813
absence of a specific subgingival microflora in adults with down's syndrome.periodontal disease in down's syndrome (ds) is generally characterized by a high degree of bone loss. bone loss of 5 mm or more is observed in 70% of these subjects. among ds subjects, considerable differences in disease progression occur. so far, no studies have been conducted in which specific properties of the subgingival microflora have been related to the condition observed.200111686820
[effect of supragingival plaque control on subgingival microflora and periodontal tissues].the aim of this study was to investigate, clinically and microbiologically, forty-four sites in 11 patients presenting with generalized chronic periodontitis. plaque index (pi), gingival index (gi), probing bleeding (pb), probing depth (pd) and insertion level (il) were registered. samples of subgingival plaque were collected in the same sites for cultivation of anaerobic bacteria and determination of microbiological morphotypes using dark field microscopy. clinical and microbiological data were ...200111705193
intra- and interspecies regulation of gene expression by actinobacillus actinomycetemcomitans luxs.the cell density-dependent control of gene expression is employed by many bacteria for regulating a variety of physiological functions, including the generation of bioluminescence, sporulation, formation of biofilms, and the expression of virulence factors. although periodontal organisms do not appear to secrete acyl-homoserine lactone signals, several species, e.g., porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum, have recently been shown to secrete a signal related ...200111705942
the localization of periodontal-disease-associated bacteria in human periodontal pockets.some gram-negative anaerobes are associated with the incidence and progression of periodontal disease. in periodontal pockets, however, the localization of those bacteria is unknown. we investigated the localization of 5 bacterial species in human periodontal pockets. fifteen teeth with a part of periodontal pockets from 10 adult periodontitis patients were obtained, and the localization of bacteria was examined immunohistochemically. positive reactions with anti-prevotella nigrescens antibody w ...200111706954
bacterial survival rate on tooth- and interdental brushes in relation to the use of toothpaste.previous studies indicated that oral hygiene aids can play a rôle in the intra-oral translocation of pathogens. the survival rate of cariogenic and periodontopathogenic species on toothbrushes, with and without toothpaste, and interdental brushes was presently investigated.200111737507
prior exposure of mice to fusobacterium nucleatum modulates host response to porphyromonas gingivalis.multiple periodontal pathogens sequentially colonize the subgingival niche during the conversion from gingivitis to destructive periodontal disease. an animal model of sequential immunization with key periodontal pathogens has been developed to determine whether t- and b-lymphocyte effector functions are skewed and fail to protect the host from pathogenic challenge. the present study was performed to evaluate the immunomodulatory effect of exposure to fusobacterium nucleatum prior to porphyromon ...200111737656
lactoferrin, amylase and mucin muc5b and their relation to the oral microflora in hyposalivation of different origins.there are several reasons for hyposalivation, each affecting the salivary composition in different ways. the aim of this study was to analyze and compare lactoferrin, amylase and mucin muc5b in stimulated whole saliva collected from subjects with hyposalivation of different origins and to relate the results to the presence of some microbial species associated with oral disorders. albumin was determined as a marker of serum leakage. the characteristic feature for subjects with radiation-induced h ...200111737657
supragingival and subgingival microbiota of adult patients with down's syndrome. changes after periodontal treatment.in this longitudinal study, five adult down's syndrome patients with periodontitis were placed on a frequent recall visit schedule (every 6 weeks) after treatment, in order to investigate: 1) the microbiological status, both supragingivally and subgingivally, and the changes that occurred after treatment and 2) the effect of frequent professional supragingival plaque control on the subgingival microbiota and clinical variables in these patients. the clinical variables recorded were probing pocke ...200111737662
[a comparative study of various evaluation methods of the antibiotic sensitivity of strict anaerobic bacteria of the subgingival flora].the study on the sensitiveness of slow-growing anaerobes bacteria to antibiotics is delicate when you consider the technical motives that make it difficult to transpose the standard methods frequently used in microbiological laboratories. the three main methods used to determine susceptibility to antibiotics are: disk-diffusion test, antibiotics containing microdilution plates and atb ana (biomérieux). the aim of this study is to compare the effectiveness of each of these methods on severe anaer ...200111808376
[brain abscess in retroperitoneal perforated colonic diverticulitis].the most frequent complications in diverticular disease are local abscess, perforation with peritoneal sepsis, fistula and ileus. extraabdominal manifestation is an actual rarity. a haematogenous bacterial spread via portal vein with formation of liver abscess has seldom been described. but a complicated diverticular disease as a cause for a brain abscess is an absolute rarity. our case presents a patient with brain abscess caused by asymptomatic, retroperitoneal perforated colonic diverticulosi ...200111824027
maternal periodontitis and prematurity. part ii: maternal infection and fetal exposure.clinical data from the first 812 deliveries from a cohort study of pregnant mothers entitled oral conditions and pregnancy (ocap) demonstrate that both antepartum maternal periodontal disease and incidence/progression of periodontal disease are associated with preterm birth and growth restriction after adjusting for traditional obstetric risk factors. in the current study we present measures of maternal periodontal infection using whole chromosomal dna probes to identify 15 periodontal organisms ...200111887461
two cases of diskitis attributable to anaerobic bacteria in children.diskitis, an inflammation of the intervertebral disk, is generally attributable to staphylococcus aureus and rarely staphylococcus epidermidis, kingella kingae, enterobacteriaciae, and streptococcus pneumoniae. in many cases, no bacterial growth is obtained from infected intervertebral discs. although anaerobic bacteria were recovered from adults with spondylodiscitis, these organisms were not reported before from children. the recovery of anaerobic bacteria in 2 children with diskitis is report ...200111158500
interleukin-6 deficiency increases inflammatory bone destruction.periapical bone destruction occurs as a consequence of pulpal infection. in previous studies, we showed that interleukin-1 (il-1) is the primary stimulator of bone destruction in this model. il-6 is a pleiotropic cytokine that is induced in these infections and has both pro- and anti-inflammatory activities. in the present study, we determined the role of il-6 in regulating il-1 expression and bone resorption. the first molars of il-6 knockouts (il-6(-/-)) and wild-type mice were subjected to su ...200111159963
comparison of the hydrophobic properties of candida albicans and candida dubliniensis.although candida dubliniensis is a close genetic relative of candida albicans, it colonizes and infects fewer sites. nearly all instances of candidiasis caused by c. dubliniensis are restricted to the oral cavity. as cell surface hydrophobicity (csh) influences virulence of c. albicans, csh properties of c. dubliniensis were investigated and compared to c. albicans. growth temperature is one factor which affects the csh status of stationary-phase c. albicans. however, c. dubliniensis, similar to ...200111159968
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...200111120995
genome sequence and analysis of the oral bacterium fusobacterium nucleatum strain atcc 25586.we present a complete dna sequence and metabolic analysis of the dominant oral bacterium fusobacterium nucleatum. although not considered a major dental pathogen on its own, this anaerobe facilitates the aggregation and establishment of several other species including the dental pathogens porphyromonas gingivalis and bacteroides forsythus. the f. nucleatum strain atcc 25586 genome was assembled from shotgun sequences and analyzed using the ergo bioinformatics suite (http://www.integratedgenomics ...200211889109
subgingival microbiota of indigenous indians of central america.to define the subgingival microbial profiles of adult subjects from a previously identified rural community of indigenous indians in guatemala, central america.200211895544
[clinical and bacteriological features of six cases with intracranial abscess in childhood].from october 1988 to march 2001, 5 patients with 6 episodes of intracranial abscesses were admitted to chiba-children's hospital. average age when they were admitted was 10 years and 1 month. initial clinical symptoms were fever in 5 cases, and headache in 1 case. it took 21 days from the appearance of the initial symptoms to diagnose the intracranial abscess. four out of 5 patients had underlying diseases that were prone to cause intracranial abscess. two patients of these were cyanotic congeni ...200211905002
clinical and microbiological studies of periodontal disease in sjögren syndrome patients.little is known about the periodontal status of patients with sjögren's syndrome (ss), a chronic inflammatory autoimmune disease characterized by xerophthalmia and xerostomia. the aim of the present study was to evaluate whether the periodontal status of ss patients, in terms of clinical and microbiological parameters, differs from systemically healthy age- and gender-matched controls.200211908470
16s-23s rdna internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus fusobacterium.the 16s-23s rdna internal transcribed spacer (its) regions of all currently defined fusobacterium species and related taxa such as leptotrichia buccalis, sebaldella termitidis and streptobacillus moniliformans, were analysed to examine inter- and intraspecies as well as subspecies relationships. for the its-amplification, a new eubacterial universal primer pair was designed and used. the majority of the fusobacterium strains, along with l. buccalis showed one major, and two to three weaker, dist ...200211931161
in vitro effect of intracanal medicaments on strict anaerobes by means of the broth dilution method.the determination of bacterial susceptibility to intracanal medicaments is a necessity. nevertheless, few studies utilize the proper methodology to carry out that evaluation with anaerobes. in this study, the steps of a broth dilution method, carried out in microplates (microdilution) and tubes (macrodilution), to test the effect of traditional intracanal medicaments on anaerobic bacteria are described. the results are presented as values of minimal inhibitory and bactericidal concentrations (mi ...200211938715
treatment of papillon-lefèvre syndrome periodontitis.conventional mechanical treatment of papillon-lefèvre syndrome periodontitis has a poor prognosis. this report describes an effective antimicrobial treatment of rapidly progressing periodontitis in an 11-year old girl having papillon-lefèvre syndrome.200211966936
effect of fusobacterium nucleatum on the t and b cell responses to porphyromonas gingivalis in a mouse model.t cell cytokine profiles and specific serum antibody levels in five groups of balb/c mice immunized with saline alone, viable fusobacterium nucleatum atcc 25586, viable porphyromonas gingivalis atcc 33277, f. nucleatum followed by p. gingivalis and p. gingivalis followed by f. nucleatum were determined. splenic cd4 and cd8 cells were examined for intracytoplasmic interleukin (il)-4, interferon (ifn)-gamma and il-10 by dual colour flow cytometry and the levels of serum anti-f. nucleatum and anti- ...200211985513
subgingival microbiota of brazilian subjects with untreated chronic periodontitis.different periodontopathogenic microbiota have been associated with periodontal diseases in several populations. the present investigation determined the subgingival microbiota of untreated chronic periodontitis brazilians using the checkerboard dna-dna hybridization technique.200211990436
enumeration of viable anaerobic bacteria by solid phase cytometry under aerobic conditions.classical enumeration methods for anaerobes are time-consuming and require special conditions. solid phase cytometry (spc) is a recent laser scanning technique for the quantitative detection of fluorescently labelled bacteria on a membrane filter that eliminates the need for a growth phase. fluorescent labelling of cells results from the cleavage by intracellular esterases of a fluorescein type ester to yield a free fluorescein derivative, which is retained only in cells with an intact cytoplasm ...200211997163
parotid salivary s-iga antibodies during experimental gingivitis in smokers and non-smokers.persons who smoke display a less pronounced increase of gingival bleeding in the experimental gingivitis model as compared with non-smokers. the aim of the present study was to investigate whether this could partly be explained by differences in levels of parotid total secretory iga (s-iga) or parotid s-iga reactive with selected oral microorganisms. parotid saliva samples were obtained from 11 smoking and 14 non-smoking volunteers, at baseline, after 5 and 14 days of full mouth experimental gin ...200212009188
mice lacking monocyte chemoattractant protein 1 have enhanced susceptibility to an interstitial polymicrobial infection due to impaired monocyte recruitment.monocyte chemoattractant protein 1 (mcp-1) is an important chemokine that induces monocyte recruitment in a number of different pathologies, including infection. to investigate the role of mcp-1 in protecting a host from a chronic interstitial polymicrobial infection, dental pulps of mcp-1(-/-) mice and controls were inoculated with six different oral pathogens. in this model the recruitment of leukocytes and the impact of a genetic deletion on the susceptibility to infection can be accurately a ...200212011011
treatment of periodontal disease in a patient with ehlers-danlos syndrome. a case report and literature review.ehlers-danlos syndrome (eds) designates a heterogeneous group of connective tissue disorders characterized by skin elasticity, tissue fragility, and chronic joint pain. dental findings have been reported with some types of eds. this case report describes the periodontal findings in a patient with a previously undiagnosed eds type viii.200212027261
effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms.antimicrobial effects of fluoride in vivo remain contentious. previous studies suggested that 1 mm naf reduced acid production from glucose, and prevented the enrichment of bacteria associated with caries in a chemostat model. the present study examines the effects of a lower fluoride concentration (0.53 mm, 10 ppm naf) in both biofilm and planktonic microbial communities. nine oral species were grown at ph 7.0 and pulsed on 10 successive days with glucose; bacterial metabolism was allowed to re ...200212037363
dipeptide utilization by the periodontal pathogens porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum.porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum, which can frequently be isolated from periodontal pockets, preferentially utilize proteins and peptides as growth substrates. in this study, we determined the size of peptide that is preferentially utilized as a source of energy and material for cell growth by p. gingivalis, p. intermedia, p. nigrescens and f. nucleatum using various sizes of poly amino acids consisting of two to approximately 100 ...200211860556
fusobacterium nucleatum in endodontic flare-ups.the extent to which fusobacterium nucleatum is recovered from root canals of teeth that present with an interappointment flare-up following endodontic instrumentation was investigated.200211862208
periodontal bacteria in rabbit mandibular and maxillary abscesses.despite the high incidence of odontogenic abscesses in pet rabbits, published data on the bacteriology of these infections are lacking, and clinical cultures are often ambiguous, making antibiotic choices difficult. in order to define the bacteriology of these infections, 12 rabbit mandibular and maxillary abscesses were cultured aerobically and anaerobically. all specimens yielded pathogenic bacteria, including fusobacterium nucleatum, prevotella heparinolytica, prevotella spp., peptostreptococ ...200211880435
[antibacterial activity of new-quinolone and macrolide antibiotics against oral bacteria].minimum inhibitory concentration (mic) of 4 new-quinolone antibacterial reagents and 4 macrolide antibiotics against periodontopathic bacteria 21 species 24 strains of standard strains including oral related bacteria and clinical isolated (1993-1999) 20 strains of porphyromonas gingivalis, 7 strains of actinobacillus actinomycetemcomitans were determined. minocycline hcl was used as the comparative reagent. macrolide showed moderate antibacterial activity against standard strains of oral related ...200212508477
periodontal microbiota and clinical periodontal status in a rural sample in southern thailand.we sought to determine (i) the association of subgingival bacterial profiles to clinical periodontal status in a population with limited access to dental care in thailand, and (ii) the external validity of our earlier findings from a similar study in rural china. we examined 356 subjects, 30-39 yr old and 50-59 yr old, with respect to clinical periodontal status and subgingival plaque at maximally 14 sites per subject. checkerboard hybridizations were used to analyse a total of 4343 samples. the ...200212664464
igg subclass specific antibody response to periodontopathic organisms in hiv-positive patients.we previously reported an increased rate of progression of periodontal disease over an 18-month period in human immunodeficiency virus (hiv)-positive subjects compared to controls. the mechanism for disease progression and rapid tissue loss was unknown. data on the microbiological studies failed to show any significant difference in the microbial characteristics of the periodontal lesions in hiv-positive patients compared to hiv-negative controls. immunological analysis had identified neutrophil ...200212546094
isotypic antibody response to plaque anaerobes in periodontal disease.it has been suggested that locally produced immunoglobulin (ig)a could be more protective than igg and that there could be a relationship between crevicular fluid-specific iga levels and the onset of periodontal disease. this study was designed to investigate this hypothesis regarding specific immune responses towards 4 plaque anaerobes in gingival crevicular fluid and saliva from patients with periodontopathies and controls.200212546101
[the influence of two implant materials on the growth of three subgingival predominant bacteria].commercially pure titanium and titanium alloy as dental implants have show impressive clinical results. despite the high success rates, some implants do fail. compared to those studies on osseointegration of implants, the information pertaining to their failure is little. further studies on the relationship between implant material and micro-organism are needed. the purpose of this investigation is to study the effect of two commonly used implant materials titanium (ta2) and ti-6ai-4v alloy (tc4 ...200212599430
[clinical and laboratory studies of bacterial adhesion to validate the choice of material for making provisional dentures for patients with periodontal diseases].adhesion of bacteria favoring the development of oral inflammations, including cariesogenic and periodontopathogenic (actinobacillus actinomycetemcommitans, streptococcus sanguis, fusobacterium nucleatum, staphylococcus warneri) and yeast fungi (candida albicans), to 13 materials used for making provisional dentures was studied. adhesion of all the studied bacteria and fungi to russian material esterfil foto was the minimum. clinical use of this material in patients with chronic generalized peri ...200212056141
direct amplification of rrna gene sequences for identification of selected oral pathogens in root canal infections.the purpose of this study was to assess the prevalence of selected oral pathogens in root canal infections and their relationship with symptoms using a highly sensitive technique, the polymerase chain reaction.200212059935
salivary microbiota levels in relation to periodontal status, experience of caries and miswak use in sudanese adults.the purpose of the present investigation was to assess the salivary levels of 25 oral bacteria in relation to periodontal status and experience of caries, and to compare the levels of these bacteria between habitual miswak and toothbrush users in adult sudanese subjects.200212060423
possible association between amniotic fluid micro-organism infection and microflora in the mouth.to determine whether oral bacteria are found in the amniotic cavity.200212066942
tales of the unexpected. 200212088653
influence of secondary colonizers and human plasma on the adherence of porphyromonas gingivalis in vitro.the influence of secondary colonizers (fusobacterium nucleatum and actinomyces naeslundii) and the effect of human plasma on the adherence of porphyromonas gingivalis were investigated. hydroxyapatite (hap) discs coated with streptococcus sanguis were immersed in a 3h-labeled bacterial cell suspension of f. nucleatum or a. naeslundii and then in a 14c-labeled p. gingivalis cell suspension. bacterial cells on the discs were pyrolysed to quantify the radioisotopes released. the cell numbers of sec ...200212120708
3-chloro-dl-alanine resistance by l-methionine-alpha-deamino-gamma-mercaptomethane-lyase activity.the antibacterial agent 3-chloro-dl-alanine (3ca) is an inhibitor of peptidoglycan synthesis. fusobacterium nucleatum and porphyromonas gingivalis, the bacteria responsible for oral malodor, are shown to be resistant to 1 mm 3ca, whereas streptococcus mutans and escherichia coli are sensitive to this antibacterial agent at the same concentration. we isolated the 3ca resistance gene from f. nucleatum and showed that the gene encodes an l-methionine-alpha-deamino-gamma-mercaptomethane-lyase that c ...200212123816
regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation.the riboflavin biosynthesis in bacteria was analyzed using comparative analysis of genes, operons and regulatory elements. a model for regulation based on formation of alternative rna structures involving the rfn elements is suggested. in gram-positive bacteria including actinomycetes, thermotoga, thermus and deinococcus, the riboflavin metabolism and transport genes are predicted to be regulated by transcriptional attenuation, whereas in most gram-negative bacteria, the riboflavin biosynthesis ...200212136096
contamination of interdental brushes by periodontopathogens.experimental studies have shown that intraoral transmission of bacteria can occur. of course, the question arises as to how this transmission may happen. in this study, the contamination of interdental brushes by periodontopathogens is examined and compared to the microbial load of the periodontal pockets. in ten untreated chronic periodontitis patients, four interdental sites were professionally brushed with one interdental brush per patient. subsequently, samples from the depths of the pockets ...200212166717
microbial profile on metallic and ceramic bracket materials.the placement of orthodontic appliances creates a favorable environment for the accumulation of a microbiota and food residues, which, in time, may cause caries or exacerbate any pre-existing periodontal disease. the purpose of the present study was to compare the total bacterial counts present on metallic and ceramic orthodontic brackets in order to clarify which bracket type has a higher plaque retaining capacity and to determine the levels of streptococcus mutans and lactobacillus spp on both ...200212169034
update on the taxonomy and clinical aspects of the genus fusobacterium.the genus fusobacterium currently includes 13 species. fusobacterium nucleatum, the most frequently encountered species in humans, is heterogeneous and currently includes 5 subspecies. a potentially new subspecies of f. nucleatum that is intrinsically quinolone-resistant and phylogenetically separate from the other 5 subspecies has been identified from dog and cat oral flora. two subspecies have been described for fusobacterium necrophorum, and a new species, fusobacterium equinum, which is rela ...200212173104
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and proteomics: a new era in anaerobic microbiology.genome sequence data provide a framework for predicting potential microbial activities; however, the proteome content of the cell dictates its response to its environment. microbiology is witnessing a major initiative to elucidate the nature of the proteome of large numbers of species. the tool driving the proteomic revolution is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. during the analysis process, proteins are ionized and separated on the basis of their mass ...200212173110
microbiological, immunological and genetic factors in family members with periodontitis as a manifestation of systemic disease, associated with hematological disorders.the microflora, immunological profiles of host defence functions, and human leukocyte antigen (hla) findings are reported for a mother, son and daughter who were diagnosed as having 'periodontitis as a manifestation of systemic diseases, associated with hematological disorders'. examinations were made of the bacterial flora from the periodontal pocket, neutrophil chemotaxis, neutrophil phagocytosis, and the genotypes (dqb1) and serotypes (dr locus) of hla class ii antigens. phenotypic analyses o ...200212200976
pcr-based identification of bacteria associated with endodontic infections.pcr primers that target the bacterial 16s rrna genes (or the tuf gene for the genus enterococcus) were used to identify 10 putative bacterial pathogens in root canals with necrotic pulp. in addition, the associations of these microorganisms with symptoms and a history of diabetes mellitus were investigated. microbial samples from the root canals of 24 teeth with necrotic pulp were included in the study. pcr with universal bacterial primers identified bacterial dna in 22 specimens; the remaining ...200212202557
[microbiological validation of the choice of basic plastic for removable dentures].adhesion of obligate and facultative anaerobic bacteria, favoring the development of oral inflammatory diseases, including the cariesogenic and periodontogenic bacteria and candida albicans fungi, isolated from patients with periodontitis, to 13 basic materials used in removable denture making, was studied. the adhesion of all bacteria (streptococcus sanguis, prevotella melangogenica, fusobacterium nucleatum, corynebacterium xerosis) and fungi to hot polymerization basic materials was the maximu ...200212224327
activity of endodontic antibacterial agents against selected anaerobic bacteria.the antimicrobial activity of substances used as antibacterial agents (solutions of 10% calcium hydroxide, camphorated paramonochlorophenol - pmcc, 2% chlorhexidine digluconate and 10% castor oil plant detergent) on anaerobic bacteria (fusobacterium nucleatum atcc 25586, prevotella nigrescens atcc 33563, clostridium perfringens atcc 13124 and bacteroidesfragilis atcc 25285), using a broth dilution technique, was evaluated in vitro. for determination of minimum inhibitory and minimum bactericide ...200212238802
sequence conservation and distribution of the fusobacterial immunosuppressive protein gene, fipa.fusobacterium nucleatum is a gram-negative anaerobe involved in various diseases, including periodontitis. recently, other investigators isolated the f. nucleatum fdc 364 fusobacterial immunosuppressive protein (fip). one subunit, fipa, impairs t-cell activation in vitro and shows homology with beta-ketothiolases. however, its distribution and variability among fusobacteria was not reported. cloned fipa gene sequences from f. nucleatum ssp. polymorphum (atcc 10953) and f. nucleatum ssp. nucleatu ...200212354214
in vitro activities of faropenem against 579 strains of anaerobic bacteria.the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ...200212384389
cloning and characterization of the l-cysteine desulfhydrase gene of fusobacterium nucleatum.hydrogen sulfide and methyl mercaptan are the two major compounds associated with oral malodor. these compounds are highly toxic, and are thought to play an important role in periodontal disease. fusobacterium nucleatum, an oral bacterium, produces large amounts of hydrogen sulfide from l-cysteine by the enzymatic action of l-cysteine desulfhydrase. we cloned and sequenced the cdl gene encoding l-cysteine desulfhydrase from f. nucleatum atcc 10953, and revealed that the structural cdl gene consi ...200212393204
recovery of anaerobic bacteria from a glossal abscess in an adolescent.glossal abscess is infrequent in children. anaerobic bacteria are rarely recovered from this infection and never have been reported in children or adolescents. a 15-year-old patient presented with a tongue abscess following trauma. aspirate of the abscess yielded polymicrobial anaerobic flora: prevotella melaninogenica, fusobacterium nucleatum, and peptostreptococcus micros. the patient recovered following incision and drainage and 14 days of antimicrobial therapy with clindamycin. this report i ...200212395007
growth ph and transient increases in amino acid availability influence polyglucose synthesis by fusobacterium nucleatum grown in continuous culture.fusobacterium nucleatum is a gram-negative anaerobe that has been implicated in the aetiology of several diseases including periodontal diseases. like other fusobacteria, it derives energy from the fermentation of amino acids and, in resting (non-growing) cells, this enables the organism to transport glucose and synthesise intracellular polyglucose (ip). the continued availability and fermentation of amino acids inhibits ip breakdown. we have grown f. nucleatum in continuous culture in a chemica ...200212399036
fusobacterium osteomyelitis in a child with sickle cell disease.children who have sickle cell disease are at increased risk for osteomyelitis caused by salmonella spp. and staphylococcus aureus. we report a case of anaerobic osteomyelitis caused by fusobacterium nucleatum in a child with sickle cell disease. the infection did not resolve with antibiotic therapy alone, but was cured after surgical debridement and hyperbaric oxygen therapy.200212400529
effect of il-1ra on human dental pulp cells and pulpal inflammation.this study was conducted to investigate the effect of interleukin-1 receptor antagonist (il-1ra) on the lps-induced interleukin-1beta (il-1beta) synthesis in human dental pulp cells and to assess the role of il-1ra in pulpal inflammation.200212406373
phenolic antibacterials from piper betle in the prevention of halitosis.piper betle l. (piperaceae) leaves which are traditionally used in india and china in the prevention of oral malodor was examined by bioassay-guided fractionation to yield allylpyrocatechol (apc) as the major active principle which showed promising activity against obligate oral anaerobes responsible for halitosis. the biological studies with apc indicated that the potential to reduce methylmercaptan and hydrogen sulfide was mainly due to the anti-microbial activity as established using dynamic ...200212413722
structural characterization of the fusobacterial non-specific porin foma suggests a 14-stranded topology, unlike the classical porins.native and recombinant foma proteins were extracted by detergent from the cell envelopes of fusobacterium nucleatum and escherichia coli, and purified to near homogeneity by chromatography. circular dichroism analysis revealed that the foma protein consists predominantly of beta-sheets, in line with the previously proposed 16-stranded beta-barrel topology model. results obtained by trypsin treatment of intact cells and cell envelopes of f. nucleatum, and from limited proteolysis of purified foma ...200212427931
antibacterial activity of moxifloxacin against periodontal anaerobic pathogens involved in systemic infections.the in vitro activity of moxifloxacin was compared with that of penicillin g, amoxycillin/clavulanate, cefoxitin, erythromycin, clindamycin and metronidazole against 158 isolates associated with periodontal infections. mic(50)/mic(90) values of moxifloxacin were respectively 0.06/0.5 mg/l for porphyromonas gingivalis (35), for prevotella spp. (28) and actinomyces spp. (35), 0.12/0.25 mg/l for fusobacterium nucleatum (20) and 0.06/0.12 mg/l for peptostreptococcus spp. (30). the minimum inhibitory ...200212458140
porphyromonas gingivalis, bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction.bacteria play an essential role in the pathogenesis of destructive periodontal disease. it has been suggested that not all bacteria associated with periodontitis may be normal inhabitants of a periodontally healthy dentition. in particular, porphyromonas gingivalis and actinobacillus actinomycetemcomitans have been isolated infrequently from subjects without periodontitis. the aim of the present study was to compare prevalence and proportions of a number of periodontal bacteria in periodontitis ...200212472995
in vitro effect of laser irradiation on cementum-bound endotoxin isolated from periodontally diseased roots.in a previous study, we evaluated the in vivo effects of an nd:yag laser on periodontal disease by measuring crevicular interleukin (il)-1beta levels before and after laser application. it was found that laser therapy was less effective than traditional scaling and root planing. these results might be due to incomplete removal of microbial residues and cementum-bound endotoxin on root surfaces by the laser. in this study, we explored the in vitro effectiveness of an nd:yag laser for the eliminat ...200212479628
a swollen neck. 200212480362
correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese.the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ...200212483235
cytokine production in response to endodontic infection in germ-free mice.this study evaluated the cytokine profiles (type 1 or type 2) that are triggered by and modulate endodontic periapical infections in the root canal system of germ-free mice. microorganisms isolated from two patients with pulpal necrosis were inoculated into two groups of experimental animals: group i (gemella morbillorum) and group ii (bifidobacterium adolescentis, fusobacterium nucleatum and clostridium butyricum). in vitro, g. morbillorum induced type 1 cytokine synthesis, while the modulation ...200212485325
similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study.this study evaluated the similarity between the oral microbiota of young children and that of their adult caregivers. oral samples from children (174 dentate and 18 pre-dentate) aged 6-36 months and their caregivers in saipan were assayed using a dna probe assay. many species including streptococcus mutans, streptococcus sobrinus, actinomyces species, campylobacter rectus, fusobacterium nucleatum, prevotella intermedia, and porphyromonas gingivalis were detected in dentate and pre-dentate childr ...200212485330
clonality of fusobacterium nucleatum in root canal infections.fusobacterium nucleatum is a gram-negative non-spore-forming, non-motile, obligate anaerobic rod that is normally isolated from the oral cavity. several studies have reported a significant heterogeneity within the f. nucleatum species. the aim of the present study was to analyze the clonal diversity of f. nucleatum strains isolated from intracanal infections and to evaluate the presence of enterobacterial repetitive intergenic consensus (eric)-like sequences in the genome of f. nucleatum. sample ...200212485332
lipopolysaccharides from periodontopathic bacteria porphyromonas gingivalis and capnocytophaga ochracea are antagonists for human toll-like receptor 4.toll-like receptors (tlrs) 2 and 4 have recently been identified as possible signal transducers for various bacterial ligands. to investigate the roles of tlrs in the recognition of periodontopathic bacteria by the innate immune system, a chinese hamster ovary (cho) nuclear factor-kappab (nf-kappab)-dependent reporter cell line, 7.7, which is defective in both tlr2- and tlr4-dependent signaling pathways was transfected with human cd14 and tlrs. when the transfectants were exposed to freeze-dried ...200211748186
regulation of human beta-defensin-2 in gingival epithelial cells: the involvement of mitogen-activated protein kinase pathways, but not the nf-kappab transcription factor family.stratified epithelia of the oral cavity are continually exposed to bacterial challenge that is initially resisted by neutrophils and epithelial factors, including antimicrobial peptides of the beta-defensin family. previous work has shown that multiple signaling pathways are involved in human beta-defensin (hbd)-2 mrna regulation in human gingival epithelial cells stimulated with a periodontal bacterium, fusobacterium nucleatum, and other stimulants. the goal of this study was to further charact ...200211751976
periapical lesion progression with controlled microbial inoculation in a type i diabetic mouse model.uncontrolled or poorly controlled diabetes mellitus may be a risk factor for the development of large and/or debilitating periapical infections. the objectives of this investigation were to: (i) determine the effect of diabetes mellitus on the pathogenesis of periapical lesions with or without specific bacterial inoculations at the exposure sites, and (ii) test the sensitivities of two microbiological techniques in detecting the persistence of the bacterial inoculum in exposed pulps of nonobese ...200211806653
fusobacterium nucleatum supports the growth of porphyromonas gingivalis in oxygenated and carbon-dioxide-depleted environments.the authors compared the differences in tolerance to oxygen of the anaerobic periodontopathic bacteria fusobacterium nucleatum and porphyromonas gingivalis, and explored the possibility that f. nucleatum might be able to support the growth of p. gingivalis in aerated and co2-depleted environments. both micro-organisms were grown as monocultures and in co-culture in the presence and absence of co2 and under different aerated conditions using a continuous culture system. at steady state, viable co ...200211832510
comparison of the effectiveness of bacterial culture, 16s rdna directed polymerase chain reaction, and checkerboard dna-dna hybridization for detection of fusobacterium nucleatum in endodontic infections.fusobacterium nucleatum is a gram-negative, non-spore-forming, nonmotile, obligatory anaerobic rod that is normally isolated from the oral cavity. epidemiological studies have shown that this species is one of the most prevalent in primary root canal infections. the purpose of this study was to compare the effectiveness of bacteriological culture, 16s rdna directed polymerase chain reaction and checkerboard dna-dna hybridization for detection of f. nucleatum strains in infected teeth associated ...200211833695
in vitro activities of the des-fluoro(6) quinolone bms-284756 against aerobic and anaerobic pathogens isolated from skin and soft tissue animal and human bite wound infections.bms-284756, a new des-fluoro(6) quinolone, was very active against 240 aerobic and 180 anaerobic isolates from bite victims. it inhibited 403 of 420 (96%) isolates, including those of moraxella spp., cdc group ef-4, and eikenella corrodens at < or = 2 microg/ml and those of all pasteurella spp. and bergeyella zoohelcum at < or = 0.015 microg/ml. fusobacterium russii and 6 of 11 fusobacterium nucleatum isolates of animal bite origin were resistant, but isolates of human bite origin were susceptib ...200211850275
[retrospective analysis of fusobacterium associated infections; experience at aomori prefectural hospital with 108 cases from 1995 to 1999].we experienced 108 cases of fusobacterium associated infections, including otolaryngeal, oral, pleuropulmonary, intraabdominal, skin and soft tissue infections, at aomori prefectural hospital during the 5 year-period from 1995 to 1999. a total of 433 organisms, included 113 fusobacterium spp. (80 fusobacterium nucleatum, 18 fusobaterium necrophorum, 5 fusobacterium varium, 4 fusobacterium mortiferum, 6 fusobacterium spp.), were recovered with an average of 4.0 organisms per case of the 108 cases ...200211852471
evaluation of antibacterial activity of mangifera indica on anaerobic dental microglora based on in vivo studies.the present study was carried out to compare the subgingival plaque microflora in mango leaf users. fifty subjects of both sexes, 25 of them used tooth brush and 25 used mango leaf as their home care hygiene device were included in the study. the microbiological evaluation for specific bacterial counts of actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum and peptostreptococcus micros were carried out for all subject. specific microbial ...200212785172
letter: laparoscopy explosion hazards with nitrous oxide. 2002127642
plasma camp and renin in diabetics during insulin induced hypoglycemia. 2002187538
electrodiagnosis in peripheral nerve injury. 2002135753
exercise and asthma: overview and clinical impact.the question of the relationship between exercise and asthma dates back to the second century a.d. and was not resolved until r.s. jones and co-authors in 1962 reported that there were in reality two effects on the asthmatic patient from exercise depending on the duration of the exercise. short-term exercise of less than four minutes resulted in bronchodilatation, whereas longer-term exercise resulted in bronchoconstriction. exercise-induced bronchospasm occurs in all ages but is more common in ...20021187272
conservative management of low back pain.with the apparent indefinite postponement of the release of chymopapain for lumbar disk disorders, it seems appropriate to review critically the proliferation of proposed methods of management of this common complaint, particularly the numerous exercise regimens which have been set forth as helpful. a critical review of these regimens quickly reveals that the majority are propounded with considerably more confidence than statistical proof of their efficacy. the following is a delineation of the ...2002130680
gtp-binding membrane proteins in activated and differentiating t cells.we have earlier reported changes in the gtp binding of several membrane proteins including gs alpha and gi alpha during thymic differentiation of t cells. using an [alpha-32p]gtp-photoaffinity labeling technique we have studied the pattern of gtp binding proteins in activated and resting t lymphocytes and in t cells induced to differentiate by tpa. the gtp binding proteins in mitogen-activated t cells resembled those seen in leukemia t cell lines. treatment of jurkat, but not of ccrf-cem, t cell ...20021906270
[characteristics of dynamic shifts in physical development of children of preschool age in leningrad]. 2002127982
rectus sheath haematoma. 2002127923
the sezary's syndrome. 2002128162
[acne vulgaris]. 2002139681
benefit-cost and cost-effectiveness analysis: theory and application.benefit-cost analysis and cost-effectivensss analysis are terms used with increasing frequency by health planners and those concerned with review and evaluation of specific programs. the economic bases for these formal techniques are however often obscured by adaptations of convenience or misapplications of the concepts by biological scientists (and others). this paper reviews briefly the theory of benefit-cost analysis and its potential as a tool in choosing programs of optimum size, of maximum ...2002118068
cricopharyngeal sphincterotomy and bilateral division of the chorda tympani in bulbar palsy. 2002128757
correlation of tissue typing, mixed lymphocyte culture, and related donor renal allograft survival.results of mixed lymphocyte culture reactions and tissue typing were correlated with the clinical courses of recipients of living related donor renal allografts. forty-nine patients tested by the mixed lymphocyte culture technique were divided into two response groups: stimulation index greater than 5 and stimulation index less than 5. seventy patients were tested by standard tissue typing methods and were categorized by the number of misstimulation in mixed lymphocyte culture than with mismatch ...2002151949
[details of a denounced physician (e.n.t.) suspected of bodily injury, and the following expert judgement (author's transl)].an otologist was denounced and the patient's case history was confiscated because after tonsillectomy an absecess and a thrombosis of the vena jugularis interna had developed. the procedure and the medical aspect how at first being judged by the lawyer and the public prosecutor are described. on the base of the given medical opinion the court quashed proceedings. because of the growing number of denouncements this casuistic paper ends with four guiding principles to protect against unjustified m ...2002135139
[morphological differentiation of neuroblastoma by 1 methyl cyclohexane carboxylic acid (cca) and some c1 derivatives].we describe the induction of neuroblastoma morphological differentiation by 1 methyl cyclohexane carboxylic acid (cca) and by some c1 derivatives of cca. this induction proceeds in a medium which allows, in the absence of inducer, a normal growth of neuroblastoma cells and contains 7.5% fetal calf serum. in order to establish a clear-dose response relationship and to compare the relative potencies of different drugs, the morphological changes were assessed by examining "long neurite-bearing cell ...2002120786
[pharmacological influence of valproate on phenobarbitone and phenytoin serum concentrations in epileptic patients (author's transl)]. 2002119918
the surgical management of low back and sciatic syndrome in disc disease or injury : results of a joint neurosurgical and orthopaedic project. 2002127575
letter: special classes. 2002127593
[surgery and surgeons of leningrad during the great patriotic war]. 2002132736
[instrumental perforations of the esophagus]. 2002139654
[parenteral hyperalimentation in the treatment of esophageal, gastric and intestinal fistulas (author's transl)]. 2002107847
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