PMID(sorted descending)
photoinactivation using visible light plus water-filtered infrared-a (vis+wira) and chlorine e6 (ce6) eradicates planktonic periodontal pathogens and subgingival biofilms.alternative treatment methods for pathogens and microbial biofilms are required due to the widespread rise in antibiotic resistance. antimicrobial photodynamic therapy (apdt) has recently gained attention as a novel method to eradicate pathogens. the aim of this study was to evaluate the antimicrobial effects of a novel apdt method using visible light (vis) and water infiltrated infrared a (wira) in combination with chlorine e6 (ce6) against different periodontal pathogens in planktonic form and ...201627965635
the danger signal extracellular atp is an inducer of fusobacterium nucleatum biofilm dispersal.plaque biofilm is the primary etiological agent of periodontal disease. biofilm formation progresses through multiple developmental stages beginning with bacterial attachment to a surface, followed by development of microcolonies and finally detachment and dispersal from a mature biofilm as free planktonic bacteria. tissue damage arising from inflammatory response to biofilm is one of the hallmark features of periodontal disease. a consequence of tissue damage is the release of atp from within t ...201627909688
high-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis.periodontal disease (pd) is generated by microorganisms. these microbes can enter the general circulation causing a bacteraemia. the result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. level a evidence supports that pd is independently associated with arterial disease. pd is a common chronic condition affecting the majority of americans 30 years of age and older. atherosclerosis remains the largest cause of death and disability. studies indicate ...201727899684
fusobacterium nucleatum: an unusual cause of subdural empyema.this article describes an uncommon presentation of a subdural empyema in a young man. because the patient's initial presentation consisted of nonspecific abdominal complaints, subdural empyema was not initially considered. however, a thorough medical history, including recent travel history, and appropriate multidisciplinary consultation, were key to reaching the correct diagnosis.201627898560
quantitative detection of periodontopathic bacteria in lower respiratory tract specimens by real-time pcr.the presence of common periodontopathic bacteria, the fusobacterium nucleatum-periodonticum-simiae group, prevotella intermedia, and porphyromonas gingivalis was determined from respiratory tract specimens of bacterial pneumonia by real-time pcr using universal and species-specific taqman probe/primer sets. 42 patients with infectious pneumonia and 45 patients without infectious pneumonia were retrospectively enrolled in clinical studies. periodontopathic bacterial dna was found in 57.1% cases o ...201727894820
porphyromonas gingivalis capsule-mediated coaggregation as a virulence factor in mixed infection with fusobacterium nucleatum.porphyromonas gingivalis capsule enables evasion from phagocytosis, invasion of keratinocytes and bacterial survival. in mixed infection, the capsule also participates in coaggregation, which may lead to characteristic virulence not present in the mono-infection. the aim of the study was to evaluate the role of p.gingivalis capsule as a virulence factor in co-aggregated mixed infection with fusobacterium nucleatum.201627885964
fusobacterium nucleatum increases proliferation of colorectal cancer cells and tumor development in mice by activating toll-like receptor 4 signaling to nuclear factor-κb, and up-regulating expression of microrna-21.nearly 20% of the global cancer burden can be linked to infectious agents. fusobacterium nucleatum promotes tumor formation by epithelial cells via unclear mechanisms. we aimed to identify micrornas (mirnas) induced by f nucleatum and evaluate their ability to promote colorectal carcinogenesis in mice.201727876571
inducible expression of a disintegrin and metalloproteinase 8 in chronic periodontitis and gingival epithelial cells.the expression of a disintegrin and metalloproteinase 8 (adam8) is associated with several inflammatory diseases. elevated adam8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. the objective of this study was to investigate adam8 expression in chronic periodontitis tissues compared with that in normal tissues. adam8 expression and its inductive mechanism were examined in human gingival epithelial cells (hgecs) and human gingival fibroblasts.201627859260
cross-sectional evaluation of periodontal status and microbiologic and rheumatoid parameters in a large cohort of patients with rheumatoid arthritis.this study evaluates periodontal conditions and microbiologic findings and their influence on rheumatologic disease parameters in patients with rheumatoid arthritis (ra).201727858553
thermoreversible gel for intrapocket delivery of green tea catechin as a local drug delivery system: an original research.the periodontal therapies along with systemic antibiotic therapy aim at eliminating the subgingival microbiota to arrest the progression of periodontal diseases. the complete elimination is often difficult, and thus the probability of repopulation after periodontal therapy is also high. the objectives of the study are to develop in situ thermoreversible gelling system of green tea catechins suitable for periodontal pocket administration, which would act as an adjunct to mechanical periodontal th ...201727833893
fusobacterium nucleatum-induced impairment of autophagic flux enhances the expression of proinflammatory cytokines via ros in caco-2 cells.fusobacterium nucleatum (f. nucleatum) plays a critical role in gastrointestinal inflammation. however, the exact mechanism by which f. nucleatum contributes to inflammation is unclear. in the present study, it was revealed that f. nucleatum could induce the production of proinflammatory cytokines (il-8, il-1β and tnf-α) and reactive oxygen species (ros) in caco-2 colorectal) adenocarcinoma cells. furthermore, ros scavengers (nac or tiron) could decrease the production of proinflammatory cytokin ...201627828984
fusobacterium nucleatum in colorectal carcinoma tissue according to tumor location.evidence suggests a possible role of fusobacterium nucleatum in colorectal carcinogenesis, especially in right-sided proximal colorectum. considering a change in bowel contents and microbiome from proximal to distal colorectal segments, we hypothesized that the proportion of colorectal carcinoma enriched with f. nucleatum might gradually increase along the bowel subsites from rectum to cecum.201627811909
effects by periodontitis on pristane-induced arthritis in infection-immune association of periodontal disease with rheumatoid arthritis has been suggested. this study aimed to investigate the effect of pre-existing periodontitis on the development and the immune/inflammatory response of pristane-induced arthritis.201627809921
 background nonsurgical periodontal treatment implicates bacteraemia. in rare cases oral pathogens can be found associated with abscesses of brain or liver case description a brain abscess was found in a patient after several periodontal treatments causing neurological seizures. in the drain masses of fusobacterium nucleatum were found. an oral examination revealed a severe periodontally damaged dentition. the woman had been in a generally healthy condition before the event. after neurosurgical t ...201627808349
quantitation of faecal fusobacterium improves faecal immunochemical test in detecting advanced colorectal neoplasia.there is a need for an improved biomarker for colorectal cancer (crc) and advanced adenoma. we evaluated faecal microbial markers for clinical use in detecting crc and advanced adenoma.201627797940
consequences of orthodontic treatment in malocclusion patients: clinical and microbial effects in adults and children.malocclusion is a common disease of oral and maxillofacial region. the study was aimed to investigate levels changes of periodontal pathogens in malocclusion patients before, during and after orthodontic treatments, and to confirm the difference between adults and children.201627793138
changes in salivary periodontal pathogens after orthodontic treatment: an in vivo prospective analyze the initial changes in salivary levels of periodontal pathogens after orthodontic treatment with fixed appliances.201627792427
comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample?the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control).201727786617
[analysis of causes and whole microbial structure in a case of rampant caries].to analyze the whole microbial structure in a case of rampant caries to provide evidence for its prevention and treatment.201627777193
human microbiome fusobacterium nucleatum in esophageal cancer tissue is associated with prognosis.fusobacterium nucleatum (f. nucleatum) is a component of the human microbiome that primarily inhabits the oral cavity. it causes periodontal disease and has also been implicated in the development of human cancers. although there are several reports of the relationship between f. nucleatum and the clinical outcome in human cancers, its prognostic significance in esophageal cancer remains unclear.201627769987
strain-specific impact of fusobacterium nucleatum on neutrophil function.neutrophil function is critical for initiation and progression of infecto-inflammatory diseases. key quorum-sensing plaque bacteria, such as fusobacterium nucleatum, act as bridging species between early and late colonizer pathogens, such as porphyromonas gingivalis, as the biofilm ages and periodontal inflammation increases. this study is designed to determine impact of different f. nucleatum strains on neutrophil function.201727762731
efficacy of various side-to-side toothbrushes and impact of brushing parameters on noncontact biofilm removal in an interdental space model.the objective of this study was to evaluate the efficacy of four different side-to-side toothbrushes and the impact of various brushing parameters on noncontact biofilm removal in an adjustable interdental space model.201627757550
light activated antimicrobial agents can inactivate oral malodour causing bacteria.oral malodour is a common condition which affects a large proportion of the population, resulting in social, emotional and psychological stress. certain oral bacteria form a coating called a biofilm on the tongue dorsum and degrade organic compounds releasing volatile sulfur compounds that are malodourous. current chemical treatments for oral malodour such as mouthwashes containing chlorhexidine or essential oils, are not sufficiently effective at reducing the bacterial load on the tongue. one p ...201627753430
bacteria in the apical root canals of teeth with apical periodontitis.bacteria in the tooth root canal may cause apical periodontitis. this study examined the bacterial species present in the apical root canal of teeth with apical periodontitis. antibiotic sensitivity tests were performed to evaluate whether these identified bacterial species were susceptible to specific kinds of antibiotics.201627745799
bacterial diversity of symptomatic primary endodontic infection by clonal analysis.the aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. samples were collected from 10 patients and submitted to bacterial dna isolation, 16s rrna gene amplification, cloning, and sequencing. a bacterial genomic library was constructed and bacterial diversity was estimated. the mean number of taxa per canal was 15, ranging from 11 to 21. a total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) w ...201627737357
salivary periodontopathic bacteria in children and adolescents with down assess and compare salivary periodontopathic bacteria between groups of down syndrome and non-down syndrome children and adolescents.201627727287
bacterial invasion into radicular dentine-an in vitro study.we wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals.201627722787
does obesity influence the subgingival microbiota composition in periodontal health and disease?to evaluate whether obesity affects the subgingival microbial composition of patients with periodontal health or chronic periodontitis (cp).201627717180
effect of bioactive dental adhesive on periodontal and endodontic pathogens.the objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. nanoparticles of amorphous ca ...201627699574
fecal bacteria act as novel biomarkers for noninvasive diagnosis of colorectal cancer.gut microbiota have been implicated in the development of colorectal cancer. we evaluated the utility of fecal bacterial marker candidates identified by our metagenome sequencing analysis for colorectal cancer diagnosis.201627697996
tea polyphenols inhibit the activation of nf-κb and the secretion of cytokines and matrix metalloproteinases by macrophages stimulated with fusobacterium nucleatum.fusobacterium nucleatum has been associated with both periodontal disease and inflammatory bowel disease. this gram-negative bacterium possesses a high inflammatory potential that may contribute to the disease process. we hypothesized that green and black tea polyphenols attenuate the inflammatory response of monocytes/macrophages mediated by f. nucleatum. we first showed that the tea extracts, egcg and theaflavins reduce the nf-κb activation induced by f. nucleatum in monocytes. since nf-κb is ...201627694921
role of oral microbiome on oral cancers, a review.the oral cavity is inhibited by many of the bacterial species. some of them have a key role in the development of oral disease. interrelationships between oral microbiome and systemic conditions such as head-and-neck cancer have become increasingly appreciated in recent years. emerging evidence also suggests a link between periodontal disease and oral cancer, and the explanation being that chronic inflammation could be a major factor in both diseases. squamous cell carcinoma is that the most fre ...201627693964
evaluation of antibody level against fusobacterium nucleatum in the serological diagnosis of colorectal cancer.fusobacterium nucleatum (f. nucleatum, fn) is associated with the colorectal cancer (crc). fn-infection could induce significant levels of serum fn-specific antibodies in human and mice. the objective of this study was to identify fn-infection that elicit a humoral response in patients with crc and evaluate the diagnostic performance of serum anti-fn antibodies. in this work, we showed the mean absorbance value of anti-fn-iga and -igg in the crc group were significantly higher than those in the ...201627678333
emerging role of bacteria in oral carcinogenesis: a review with special reference to perio-pathogenic bacteria.oral cancer, primarily oral squamous cell carcinoma (oscc), continues to be a major global health problem with high incidence and low survival rates. while the major risk factors for this malignancy, mostly lifestyle related, have been identified, around 15% of oral cancer cases remain unexplained. in light of evidence implicating bacteria in the aetiology of some cancer types, several epidemiological studies have been conducted in the last decade, employing methodologies ranging from traditiona ...201627677454
novel bioactive nanocomposite for class-v restorations to inhibit periodontitis-related pathogens.the occurrence of tooth root caries is increasing as the world population ages and tooth retention in seniors increases. class v restorations with subgingival margins are difficult to clean and often lead to periodontitis. the objectives of this study were to develop a class v composite containing dimethylaminohexadecyl methacrylate (dmahdm) and nanoparticles of amorphous calcium phosphate (nacp), and investigate mechanical properties and the inhibition of six species of periodontitis-related bi ...201627671471
microbial characteristics of peri-implantitis: a case-control study.the aim of this case-control study is to compare oral microbiologic characteristics of patients with healthy peri-implant conditions and patients with peri-implantitis and to explore the influence of various patient- and implant-related factors on microbiologic characteristics.201727666672
discovery and characterization of de novo sialic acid biosynthesis in the phylum fusobacterium.sialic acids are nine-carbon backbone carbohydrates found in prominent outermost positions of glycosylated molecules in mammals. mimicry of sialic acid (n-acetylneuraminic acid, neu5ac) enables some pathogenic bacteria to evade host defenses. fusobacterium nucleatum is a ubiquitous oral bacterium also linked with invasive infections throughout the body. we employed multidisciplinary approaches to test predictions that f. nucleatum engages in de novo synthesis of sialic acids. here we show that f ...201627613803
gut microbiota, inflammation, and colorectal cancer.colorectal cancer is the second-leading cause of cancer-related deaths in the united states and fourth-leading cause of cancer-related deaths worldwide. while cancer is largely considered to be a disease of genetic and environmental factors, increasing evidence has demonstrated a role for the microbiota (the microorganisms associated with the human body) in shaping inflammatory environments and promoting tumor growth and spread. herein, we discuss both human data from meta'omics analyses and dat ...201627607555
effect of helicobacter pylori infection on chronic periodontitis by the change of microecology and inflammation.helicobacter pylori (h. pylori), a pathogen inducing peptic disease, is recently found to be binding to the progress of periodontitis. most previous studies are case-controlled, and they investigate the risk of h. pylori infection in disease the development of while few studies evaluate the correlation between h. pylori and periodontal pathogens. therefore, we investigated the correlation between h. pylori infection with periodontal parameters, periodontal pathogens and inflammation. the results ...201627602578
adhesion of periodontal pathogens to self-ligating orthodontic brackets: an in-vivo prospective study.our aims were to analyze adhesion of periodontopathogens to self-ligating brackets (clarity-sl [csl], clippy-c [cc] and damon q [dq]) and to identify the relationships between bacterial adhesion and oral hygiene indexes.201627585775
novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating.the purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., porphyromonas gingivalis (pg), fusobacterium nucleatum (fn), prevotella intermedia (pi), and aggregatibacter actinomycetemcomitans (aa)]. tetracycline hydrochloride (tch) was added to a poly(dl-lactide) [pla], poly(ε-caprolactone) [pcl], and gelatin [gel] polymer blend solution at distinct concentrations to obtain the follow ...201627585669
d-galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens.autoinducer 2 (ai-2) is a quorum sensing molecule to which bacteria respond to regulate various phenotypes, including virulence and biofilm formation. ai-2 plays an important role in the formation of a subgingival biofilm composed mostly of gram-negative anaerobes, by which periodontitis is initiated. the aim of this study was to evaluate d-galactose as an inhibitor of ai-2 activity and thus of the biofilm formation of periodontopathogens. in a search for an ai-2 receptor of fusobacterium nuclea ...201627572513
antibacterial and antibiofilm activities of docosahexaenoic acid (dha) and eicosapentaenoic acid (epa) against periodontopathic bacteria.docosahexaenoic acid (dha) and eicosapentaenoic acid (epa) are two major omega-3 polyunsaturated fatty acids (n-3 pufas) with antimicrobial properties. in this study, we evaluated the potential antibacterial and antibiofilm activities of dha and epa against two periodontal pathogens, porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum). mtt assay showed that dha and epa still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 μm ...201627565090
bacterial colonization of the peri-implant sulcus in dentate patients: a prospective observational study.the aim of the present study was to compare the composition of the periodontal microflora at baseline (t0) with the submucosal microflora at least 1 year after implant placement (t1) in periodontally healthy patients.201727558381
immunologic characteristics of human gingival fibroblasts in response to oral bacteria.there is ample evidence that gingival fibroblasts (gfs) participate in the immune response to oral bacteria and serve as immune-regulatory cells. the objective of this study was to investigate the innate immune response of gfs to oral bacteria.201627558278
development of a cavity disinfectant containing antibacterial monomer experimental cavity disinfectant (acc) that is intended to be used for various direct and indirect restorations was prepared by adding an antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (mdpb) at 5% into 80% ethanol. the antibacterial effectiveness of acc and its influences on the bonding abilities of resin cements were investigated. to examine the antibacterial activity of unpolymerized mdpb, the minimum inhibitory and bactericidal concentrations (mic and mbc) were determine ...201627558263
longitudinal study on clinical and microbial analysis of periodontal status in pregnancy.this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ...201627556678
impact of aging on trem-1 responses in the periodontium: a cross-sectional study in an elderly population.aging is associated with altered immune response, which increases susceptibility to infections. strem-1 is involved in the amplification of the inflammatory response to bacterial infection. the present cross-sectional study aims to investigate local strem-1 levels in gingival crevicular fluid (gcf) as well as key periodontal pathogen levels in the subgingival plaque in an elderly cohort with periodontal health, gingivitis, and chronic periodontitis (cp).201627542376
comparative genome analysis of fusobacterium nucleatum.fusobacterium nucleatum is considered to be a key oral bacterium in recruiting periodontal pathogens into subgingival dental plaque. currently f. nucleatum can be subdivided into five subspecies. our previous genome analysis of f. nucleatum w1481 (referred to hereafter as w1481), isolated from an 8-mm periodontal pocket in a patient with chronic periodontitis, suggested the possibility of a new subspecies. to further investigate the biology and relationships of this possible subspecies with othe ...201627540086
distinguishing the signals of gingivitis and periodontitis in supragingival plaque: a cross-sectional cohort study in malawi.periodontal disease ranges from gingival inflammation (gingivitis) to the inflammation and loss of tooth-supporting tissues (periodontitis). previous research has focused mainly on subgingival plaque, but supragingival plaque composition is also known to be associated with disease. quantitative modeling of bacterial abundances across the natural range of periodontal severities can distinguish which features of disease are associated with particular changes in composition. we assessed a cross-sec ...201627520811
oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study.polymorphonuclear neutrophils (pmns) have a major role in the innate immune system. however, little is known about pmn contribution in relation to oral health. the objective of this study was to investigate the numbers and functional characteristics of oral pmns (opmns) compared with circulatory pmns (cpmns). oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. pmn counts, cell cycle analysis and cellular ...201627515277
fap2 mediates fusobacterium nucleatum colorectal adenocarcinoma enrichment by binding to tumor-expressed gal-galnac.fusobacterium nucleatum is associated with colorectal cancer and promotes colonic tumor formation in preclinical models. however, fusobacteria are core members of the human oral microbiome and less prevalent in the healthy gut, raising questions about how fusobacteria localize to crc. we identify a host polysaccharide and fusobacterial lectin that explicates fusobacteria abundance in crc. gal-galnac, which is overexpressed in crc, is recognized by fusobacterial fap2, which functions as a gal-gal ...201627512904
[intramedullary abscess of the cervical spinal cord caused by advanced periodontitis:case report].we describe the case of a 60-year-old man with an intramedullary abscess of the cervical spinal cord caused by advanced periodontitis. he suddenly developed severe neck pain and rapidly progressive palsy of the left upper arm. t2-weighted sagittal magnetic resonance imaging(mri)revealed a hyperintense area extending from c1 to c6. gadolinium-enhanced t1-weighted mri showed a ring-enhanced lesion at the c3-4 level that was hyperintense on diffusion-weighted mri. the patient underwent drainage of ...201627506846
detection of selected periodontal bacteria in preschool children affected by early childhood caries.the aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ecc) with and without gingival inflammation. a convenience sample of 25 preschool children (mean age 3.61 years, sd 1.42) was recruited. dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the stomagene® (protean s.r.o. czech republic) and parocheck® 20 (greiner bio-one gmbh, germany) detection ...201627485551
nucleases from prevotella intermedia can degrade neutrophil extracellular traps.periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. these bacteria are able to colonize the periodontal region by evading the host immune response. neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (nets). these are extracellular net-like fibers comprising dna and antimicrobial components such as histones, ll-37, defensins, myeloperoxidase, and neu ...201627476978
an assessment of early colonisation of implant-abutment metal surfaces by single species and co-cultured bacterial periodontal pathogens.numerous studies have proposed that smooth metal surfaces reduce initial bacterial attachment in the establishment of an early biofilm formation. however, these studies have largely examined single bacterial species, which are not always relevant as pathogens identified as initiators of inflammatory peri-implantitis. this study investigated the adherence of four periodontally-relevant bacterial species to implant and abutment surfaces in current clinical use.201627472955
bacteria prevalence in a large italian population sample: a clinical and microbiological study.the present study detects those bacterial species which are more strongly related to bleeding on probing, suppuration and smoking in periodontal-affected patients. nine hundred and fifty-one patients with periodontal diseases were admitted to the department of periodontology and implantology, dental school of bologna university where they underwent microbiological tests for six periodontal pathogens (actinomyces actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, treponema de ...201627469569
bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas.the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ...201627469562
evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study.the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ...201627469559
impact of implant-abutment connection on osteoimmunological and microbiological parameters in short implants: a randomized controlled clinical trial.the study aimed to determine the levels of soluble receptor activator of nuclear factor-кb ligand (srankl) and osteoprotegerin (opg) as well as their relative calculated ratio in peri-implant crevicular fluid (picf) obtained around two different types of implant-abutment connection on short implants following a 12-month monitoring period. moreover, the levels of a number of oral bacterial species were investigated in the corresponding submucosal biofilm samples.201627469171
anti-biofilm activities from resveratrol against fusobacterium nucleatum.fusobacterium nucleatum is a gram-negative, anaerobic bacterium that plays an important role in dental plaque biofilm formation. in this study, we evaluate the effect of resveratrol, a phytoalexin compound, on f. nucleatum biofilm formation. the effects of different concentrations of resveratrol on biofilms formed on 96-well microtiter plates at different time points were determined by the mtt assay. the structures and thicknesses of the biofilm were observed by confocal laser scanning microscop ...201627458454
broad-range pcr coupled with electrospray ionization time of flight mass spectrometry for detection of bacteremia and fungemia in patients with neutropenic fever.infection is an important complication in patients with hematologic malignancies or solid tumors undergoing intensive cytotoxic chemotherapy. in only 20 to 30% of the febrile neutropenic episodes, an infectious agent is detected by conventional cultures. in this prospective study, the performance of broad-range pcr coupled with electrospray ionization time of flight mass spectrometry (pcr/esi-ms) technology was compared to conventional blood cultures (bc) in a consecutive series of samples from ...201627440820
alteration of neutrophil reactive oxygen species production by extracts of devil's claw (harpagophytum).harpagophytum, devil's claw, is a genus of tuberiferous xerophytic plants native to southern africa. some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. the objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of various harpagophytum taxa in suppressing respiratory burs ...201627429708
profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws.microbial etiology for anti-osteoclastic drug-related osteonecrosis of the jaw (aronj) was suggested. this study investigates any link between bacteria colonizing aronj sites and other oral cavity sites. microbiota samples of 10 aronj patients were collected from the exposed bone, adjacent teeth, contralateral teeth, and tongue. dna checkerboard hybridization was used for microbiota analysis with 43 genomic dna probes prepared from human oral bacterial (38) and candida (5) species, using socrans ...201627419922
preventive effects of houttuynia cordata extract for oral infectious diseases.houttuynia cordata (hc) (saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in japan. our recent survey showed that hc poultice (hcp) prepared from smothering fresh leaves of hc had been frequently used for the treatment of purulent skin diseases with high effectiveness. our experimental study also demonstrated that ethanol extract of hcp (ehcp) has antibacterial, antibiofilm, and anti-inflammatory effects against s. aureus which ca ...201627413739
interactions between lactobacillus rhamnosus gg and oral micro-organisms in an in vitro biofilm model.probiotics have shown favourable properties in maintaining oral health. by interacting with oral microbial communities, these species could contribute to healthier microbial equilibrium. this study aimed to investigate in vitro the ability of probiotic lactobacillus rhamnosus gg ( to integrate in oral biofilm and affect its species composition. five oral strains, streptococcus mutans, streptococcus sanguinis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum and candida albican ...201627405227
rose bengal uptake by e. faecalis and f. nucleatum and light-mediated antibacterial activity measured by flow cytometry.antibacterial photodynamic therapy (apdt) using rose bengal (rb) and blue-light kills bacteria through the production of reactive oxygen derivates. however, the interaction mechanism of rb with bacterial cells remains unclear. this study investigated the uptake efficiency and the antibacterial activity of blue light-activated rb against enterococcus faecalis and fusobacterium nucleatum. spectrophotometry and epifluorescence microscopy were used to evaluate binding of rb to bacteria. the antibact ...201627394008
differential matrix metalloprotease (mmp) expression profiles found in aged gingiva.the periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. here, we investigated age-related genetic changes in gingiva at the transcriptomic level. genes that were differentially expressed between young and old human gingiva were identified by rna sequencing and verified by real-time pcr. a total of 1939 mrna transcripts showed significantly differential expression between young and old gingival tissues. matrix metalloprotease (mmp) regulatio ...201627391467
bone remodeling around implants with external hexagon and morse-taper connections: a randomized, controlled, split-mouth, clinical evaluate clinical, radiographic, microbiologic, and biomechanical parameters related to bone remodeling around implants with external hexagon (eh) and morse-taper (mt) connections.201727353076
rapid detection of nusg and fada in fusobacterium nucleatum by loop-mediated isothermal amplification.fusobacterium nucleatum is associated with various human diseases such as periodontal disease and colorectal cancer (crc); thus, f. nucleatum detection might serve as a novel diagnostic tool. here, we describe the development of a sensitive and rapid molecular method for detecting two f. nucleatum genes: the highly conserved nusg and fada, which encode a critical host colonization factor. loop-mediated isothermal amplification (lamp) primer sets for the rapid detection of nusg and fada were desi ...201627339262
microbiological efficacy of photodynamic therapy as an adjunct to non-surgical periodontal treatment: a clinical trial.the efficiency of routine scaling and root planning is negatively influenced by the tooth anatomy and residual bacteria all possibly affecting the treatment outcomes in future. the present study compared the microbiologic effectiveness of the photodynamic therapy (pdt) as an adjunctive treatment modality for nonsurgical treatment in chronic periodontitis.201627330709
could gut microbiota serve as prognostic biomarker associated with colorectal cancer patients' survival? a pilot study on relevant mechanism.evidences have shown that dysbiosis could promote the progression of colorectal cancer (crc). however, the association of dysbiosis and prognosis of crc is barely investigated. therefore, we used 16s rrna gene sequencing approach to determine differences in microbiota among tumor tissues of different prognosis and found that fusobacterium nucleatum and bacteroides fragilis were more abundant in worse prognosis groups, while faecalibacterium prausnitzii displayed higher abundance in survival grou ...201627323816
caveolin-1 serves as a negative effector in senescent human gingival fibroblasts during fusobacterium nucleatum is well established that aging is associated with increased susceptibility to infectious diseases. fusobacterium nucleatum is a well-known bacterial species that plays a central bridging role between early and late colonizers in the human oral cavity. further, the ability of f. nucleatum to invade gingival fibroblasts (gfs) is critical to the development of periodontal diseases. however, the mechanisms underlying the age-related infection of gfs by f. nucleatum remain unknown. we used young ( ...201627315395
quantitative molecular detection of 19 major pathogens in the interdental biofilm of periodontally healthy young oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. the interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. this pcr study is the first to describe the interdental microbiota in hea ...201627313576
metabolic and community synergy of oral bacteria in colorectal cancer.the oral periodontopathic bacterium fusobacterium nucleatum has been repeatedly associated with colorectal tumors. molecular analysis has identified specific virulence factors that promote tumorigenesis in the colon. however, other oral community members, such as members of the porphyromonas spp., are also found with f. nucleatum on colonic tumors, and thus, narrow studies of individual pathogens do not take community-wide virulence properties into account. a broader view of oral bacterial physi ...201727303740
the myeloid immune signature of enterotoxigenic bacteroides fragilis-induced murine colon tumorigenesis.enterotoxigenic bacteroides fragilis (etbf), a human commensal and candidate pathogen in colorectal cancer (crc), is a potent initiator of interleukin-17 (il-17)-dependent colon tumorigenesis in min(apc+/-) mice. we examined the role of il-17 and etbf on the differentiation of myeloid cells into myeloid-derived suppressor cells (mdscs) and tumor-associated macrophages, which are known to promote tumorigenesis. the myeloid compartment associated with etbf-induced colon tumorigenesis in min mice w ...201727301879
putative periodontopathic bacteria and herpesviruses in pregnant women: a case-control study.little is known about herpesvirus and putative periodontopathic bacteria in maternal chronic periodontitis. the present case-control study aimed to explore the potential relationship between putative periodontopathic bacteria and herpesviruses in maternal chronic periodontitis.saliva samples were collected from 36 pregnant women with chronic periodontitis (cases) and 36 pregnant women with healthy periodontal status (controls). six putative periodontopathic bacteria (porphyromonas gingivalis [pg ...201627301874
molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease.dogs commonly serve as a model for various human conditions, including periodontal diseases. the aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (rt-pcr)-based tests and to compare the results obtained in each species. bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. five samples were c ...201727297417
5-fluorouracil sensitivity varies among oral micro-organisms.5-fluorouracil (5-fu), a commonly used chemotherapeutic agent, often causes oral mucositis, an inflammation and ulceration of the oral mucosa. micro-organisms in the oral cavity are thought to play an important role in the aggravation and severity of mucositis, but the mechanisms behind this remain unclear. although 5-fu has been shown to elicit antibacterial effects at high concentrations (>100 µm), its antibacterial effect at physiologically relevant concentrations in the oral cavity is unknow ...201627296270
morphological and physiological changes induced by contact-dependent interaction between candida albicans and fusobacterium nucleatum.candida albicans and fusobacterium nucleatum are well-studied oral commensal microbes with pathogenic potential that are involved in various oral polymicrobial infectious diseases. recently, we demonstrated that f. nucleatum atcc 23726 coaggregates with c. albicans sn152, a process mainly mediated by fusobacterial membrane protein radd and candida cell wall protein flo9. the aim of this study was to investigate the potential biological impact of this inter-kingdom interaction. we found that f. n ...201627295972
a protein-repellent and antibacterial nanocomposite for class-v restorations to inhibit periodontitis-related pathogens.the objectives of this study were to develop a bioactive dental composite and investigate the effects of 2-methacryloyloxyethyl phosphorylcholine (mpc) and dimethylaminohexadecyl methacrylate (dmahdm) in class v composite on mechanical properties, water sorption, protein adsorption, and inhibition of four species of periodontitis-related biofilms for the first time. the resin consisted of ethoxylated bisphenol a dimethacrylate (ebpadma) and pyromellitic glycerol dimethacrylate (pmgdm). dmahdm, m ...201627287170
adjunctive use of essential oils following scaling and root planing -a randomized clinical trial.hitherto no study has been published on the effect of the adjunctive administration of essential oils following scaling and root planing (srp). this study describes the effect of a mouthrinse consisting of essential oils (cymbopogon flexuosus, thymus zygis and rosmarinus officinalis) following srp by clinical and microbiological variables in patients with generalized moderate chronic periodontitis.201627266517
development of novel electrospun dual-drug fiber mats loaded with a combination of ampicillin and metronidazole.our study was performed with the aim of preparing electrospun polylactide fibers with a combination of ampicillin (amp) and metronidazole (mnz) and investigating their drug release behavior and the antibacterial effect on aggregatibacter actinomycetemcomitans and other oral pathogens.201627262200
24-hour evaluation of dental plaque bacteria and halitosis after consumption of a single placebo or dental treat by dogs.objective to determine whether consumption of a single dental treat with specific mechanical properties and active ingredients would provide a 24-hour effect on dental plaque bacteria and halitosis in dogs. animals 10 dogs of various breeds from a privately owned colony that had received routine dental scaling and polishing 4 weeks before the study began. procedures dogs were randomly assigned to receive 1 placebo or dental treat first. a 4-week washout period was provided, and then dogs receive ...201627227499
global tlr2 and 4 deficiency in mice impacts bone resorption, inflammatory markers and atherosclerosis to polymicrobial infection.toll-like-receptors (tlrs) play a significant role in the generation of a specific innate immune response against invading pathogens. tlr2 and tlr4 signaling contributes to infection-induced inflammation in periodontal disease (pd) and atherosclerosis. observational studies point towards a relationship between pd and atherosclerosis, but the role of tlr2 and tlr4 in the recognition of multiple oral pathogens and their modulation of host response leading to atherosclerosis are not clear. we evalu ...201627224005
withaferin a inhibits inflammatory responses induced by fusobacterium nucleatum and aggregatibacter actinomycetemcomitans in macrophages.periodontitis is a progressive chronic inflammatory disease and a major cause of tooth loss in humans. as a withanolides, withaferin a (wa) is known to exhibit strong anti‑inflammatory activity. the present study examined whether wa inhibited inflammatory responses in macrophages in response to two representative periodontal pathogens, fusobacterium nucleatum and aggregatibacter actinomycetemcomitans. murine bone marrow‑derived macrophages (bmdms) were used in this study and cytokine production ...201627220676
fusobacterium nucleatum: atypical organism of pyogenic liver abscess might be related to sigmoid diverticulitis.pyogenic liver abscesses (plas) are the most common form of liver abscesses in the united states. most cases are caused by enteric bacteria and anaerobes. we report a case of pla caused by a rare pathogen, fusobacterium nucleatum, from an unusual primary site of infection.201627213146
endothelial cell response to fusobacterium nucleatum.vascular response is an essential aspect of an effective immune response to periodontal disease pathogens, as new blood vessel formation contributes to wound healing and inflammation. gaining a greater understanding of the factors that affect vascular response may then contribute to future breakthroughs in dental medicine. in this study, we have characterized the endothelial cell response to the common bacterium fusobacterium nucleatum, an important bridging species that facilitates the activity ...201627185790
target identification in fusobacterium nucleatum by subtractive genomics approach and enrichment analysis of host-pathogen protein-protein interactions.fusobacterium nucleatum, a well studied bacterium in periodontal diseases, appendicitis, gingivitis, osteomyelitis and pregnancy complications has recently gained attention due to its association with colorectal cancer (crc) progression. treatment with berberine was shown to reverse f. nucleatum-induced crc progression in mice by balancing the growth of opportunistic pathogens in tumor microenvironment. intestinal microbiota imbalance and the infections caused by f. nucleatum might be regulated ...201627176600
neutrophil superoxide release and plasma c-reactive protein levels pre- and post-periodontal determine peripheral blood neutrophil superoxide release and c-reactive protein (crp) concentration in chronic periodontitis patients, before and after non-surgical periodontal treatment.201627168055
microbial colonization of the peri-implant sulcus and implant connection of implants restored with cemented versus screw-retained superstructures: a cross-sectional study.the aim of this study is to investigate peri-implant and intraconnection microflora of healthy implants restored with cemented and screwed superstructures.201627153291
a case of liver abscess with systemic infection caused by fusobacterium.a 43-year-old man was admitted to our hospital because of an abnormal shadow on chest x-ray. blood testing showed elevated levels of c-reactive protein and white blood cells. computed tomography revealed multilocular masses of the right hepatic lobe, reticulonodular shadowing on both lungs, left kidney masses, and aortic arch aneurysm. fusobacterium nucleatum was isolated from the hepatic abscess after percutaneous transhepatic drainage. because of severe dental caries, he was diagnosed with liv ...201627151479
effects of lactoferrin and lactoperoxidase-containing food on the oral hygiene status of older individuals: a randomized, double blinded, placebo-controlled clinical trial.lactoferrin and lactoperoxidase have antimicrobial effects against oral pathogens. this randomized, double-blinded, placebo-controlled parallel group study tested the efficacy of a lactoferrin and lactoperoxidase-containing tablet (lf + lpo tablet) in improving the oral hygiene status of older individuals.201627150460
detection of five potentially periodontal pathogenic bacteria in peri-implant disease: a comparison of pcr and real-time pcr.the aim of this study was to compare the microbial analysis methods of polymerase chain reaction (pcr) and real-time pcr (rt-pcr) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using pcr (dna-strip technology) and rt-pcr (fluorescent dye sybr green-system): aggregatibacter actinomycetemcomitans (aa ...201627142589
invasive fusobacterium nucleatum may play a role in the carcinogenesis of proximal colon cancer through the serrated neoplasia pathway.the prevalence of invasive fusobacterium nucleatum (fn) within the serrated neoplasia pathway of the proximal colon has seldom been investigated. we examined the invasive fn and bacterial biofilms in 35 proximal hyperplastic polyps (hps), 33 sessile serrated adenomas (ssas), 48 proximal colorectal cancers (crcs) and 10 matched metastatic lymph nodes using 16s rrna fluorescence in situ hybridization (fish). samples of normal mucosa, traditional adenomas (tas), distal hps, distal crcs and matched ...201627130618
highly sensitive stool dna testing of fusobacterium nucleatum as a marker for detection of colorectal tumours in a japanese population.background accumulating evidence shows an over-abundance of fusobacterium nucleatum in colorectal tumour tissues. although stool dna testing of fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting fusobacterium nucleatum in stool by conventional methods prevented further explorations. therefore, we developed a droplet digital polymerase chain reaction (pcr) assay for detecting fusobacterium nucleatum in stool and investigated it ...201727126270
copaifera reticulata oleoresin: chemical characterization and antibacterial properties against oral pathogens.oral infections such as periodontitis and tooth decay are the most common diseases of humankind. oleoresins from different copaifera species display antimicrobial and anti-inflammatory activities. copaifera reticulata is the commonest tree of this genus and grows abundantly in several brazilian states, such as pará, amazonas, and ceará. the present study has evaluated the chemical composition and antimicrobial potential of the copaifera reticulata oleoresin (cro) against the causative agents of ...201627118478
two foxp3(+)cd4(+) t cell subpopulations distinctly control the prognosis of colorectal cancers.cd4(+) t cells that express the forkhead box p3 (foxp3) transcription factor function as regulatory t (treg) cells and hinder effective immune responses against cancer cells. abundant treg cell infiltration into tumors is associated with poor clinical outcomes in various types of cancers. however, the role of treg cells is controversial in colorectal cancers (crcs), in which foxp3(+) t cell infiltration indicated better prognosis in some studies. here we show that crcs, which are commonly infilt ...201627111280
nox1/2 activation in human gingival fibroblasts by fusobacterium nucleatum facilitates attachment of porphyromonas gingivalis.periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. sequential colonization of a broad range of bacteria, including fusobacterium nucleatum and porphyromonas gingivalis, is an important phenomenon in this disease model. f. nucleatum is a facultative anaerobic species thought to be a key mediator of dental plaque maturation due to its extensive coaggregation with other oral bacteria, while p. gin ...201627071620
bifidobacteria inhibit the growth of porphyromonas gingivalis but not of streptococcus mutans in an in vitro biofilm model.there is growing interest in the use of probiotic bifidobacteria for enhancement of the therapy, and in the prevention, of oral microbial diseases. however, the results of clinical studies assessing the effects of bifidobacteria on the oral microbiota are controversial, and the mechanisms of actions of probiotics in the oral cavity remain largely unknown. in addition, very little is known about the role of commensal bifidobacteria in oral health. our aim was to study the integration of the probi ...201627061393
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