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polarization of porphyromonas gingivalis-specific helper t-cell subsets by prior immunization with fusobacterium nucleatum.antigen-specific t-cell clones were obtained from mice immunized with fusobacterium nucleatum atcc 10953 and/or porphyromonas gingivalis 381. 10 balb/c mice per group were immunized with f. nucleatum followed by p. gingivalis, or with p. gingivalis alone by intraperitoneal injection of viable microorganisms. spleen t cells were isolated and stimulated in vitro with viable p. gingivalis cells to establish p. gingivalis-specific t-cell clones. t-cell phenotypes and cytokine profiles were determine ...200011154401
attachment of fusobacterium nucleatum pk1594 to mammalian cells and its coaggregation with periodontopathogenic bacteria are mediated by the same galactose-binding adhesin.it has been shown that fusobacterium nucleatum pk1594 coaggregates with prophyromonas gingivalis pk1924 through a galactose-binding adhesin. in the present study, attachment of f. nucleatum pk1594 to a variety of mammalian cells was characterized. f. nucleatum pk1594 attached to all eukaryotic cells tested, including human buccal epithelial cells, gingival and periodontal ligament fibroblasts, hela cells and murine lymphocytes, macrophages, and polymorphonuclear leukocytes. these attachments wer ...200011154434
porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets.recent evidence has established an association between chronic periodontitis and cardiovascular disease. periodontitis is a chronic inflammatory disease caused by a small group of gram-negative bacteria, of which porphyromonas gingivalis is considered an important causative agent. it has been proposed that dental plaque bacteria and their products can disseminate into the bloodstream from the site of infection and promote thromboembolic events associated with atherosclerosis and myocardial infar ...200011154438
profile of subgingival microbiota in children with mixed dentition.a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...200011155173
identification and characterization of human immunoglobulin g fc receptors of fusobacterium nucleatum.several human pathogens express components which can bind to the fc portion of immunoglobulins. this study was undertaken to characterize the human immunoglobulin g (igg) fc-binding activity of fusobacterium nucleatum, a suspected pathogen involved in periodontal diseases. fc-binding activity was detected using whole-cell, cell envelope and outer membrane fractions, and it was found to be associated with polypeptides of 40 kda and 42 kda, respectively. amino terminal sequencing of these componen ...200011155175
peptostreptococcus micros coaggregates with fusobacterium nucleatum and non-encapsulated porphyromonas gingivalis.coaggregation is one of the potential colonization strategies of oral microorganisms, often involving fimbrial structures in the interactions. in this study, the coaggregation characteristics of the rough and smooth genotypes of the periodontal pathogen peptostreptococcus micros were compared to investigate the role of the fibril-like structures of the rough genotype in coaggregation. of the 11 oral species tested, only fusobacterium nucleatum strains and non-encapsulated porphyromonas gingivali ...200010612731
[the neutralizing effect of histidine-rich-polypeptides on lps from suspected periodontal pathogens].human parotid histidine-rich-polypeptides (hrps) are a family of lowmolecular-weight, cationic polypeptides. hrp-1, hrp-3 and hrp-5 comprise 85%-90% of the total hrps and are called major hrps. there were many researches indicating the antimicrobial activities of hrps. recently, hrps were reported to have an inhibitory action on the lipopolysaccharide (lps) of e. coli, with hrp-5 being the most effective one among the three major hrps. since the lps of oral gram-negative bacteria is thought to b ...200012539645
isolation of fusobacteria from the oral cavities of malnourished nigerian children living in agricultural and herding villages.a previous study demonstrated the presence and possible involvement of fusobacterium necrophorum in the pathogenesis of noma lesions of children living in agricultural and herding villages in northwestern nigeria. in order to determine if f. necrophorum was part of the oral flora of malnourished children with no noma lesions, a study of the fusobacteria present in the oral cavities of 30 children, 2-6 years of age in sokoto state, was undertaken. swabs taken of the oral cavity were cultured on s ...200010702787
induction of apoptotic cell death in peripheral blood mononuclear and polymorphonuclear cells by an oral bacterium, fusobacterium nucleatum.it is largely unknown why a variety of bacteria present in the oral cavity are capable of establishing themselves in the periodontal pockets of nonimmunocompromised individuals in the presence of competent immune effector cells. in this paper we present evidence for the immunosuppressive role of fusobacterium nucleatum, a gram-negative oral bacterium which plays an important role in the generation of periodontal disease. our studies indicate that the immunosuppressive role of f. nucleatum is lar ...200010722579
[effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide].to elucidate the effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide (lps).200011780236
[antimicrobial effects of tea tree oil (melaleuca alternifolia) on oral microorganisms].the essential oil of melaleuca alternifolia (tea tree oil) exhibits antimicrobial activity against a wide range of gram-positive and gram-negative bacteria, yeasts and fungi. in this study the bacteriostatic and bacteriocidal/fungicidal activity of a tea tree oil solution, of a new tea tree oil (tebodont) and the respective placebo-gel, of a chlorhexidindigluconate-solution and of plakout was tested in vitro against ten different oral microorganisms. minimum inhibitory concentrations were in the ...200011374358
disinfection of dental impressions and occlusal records by ultraviolet radiation.as chemical disinfection of dental impressions may cause adverse effects on materials and the dental personnel this study examined disinfection by ultraviolet radiation. alginate, addition silicone rubber and red wax contaminated by streptococcus salivarius, fusobacterium nucleatum and five other bacteria in different suspension media were radiated for up to 18 min, and the number of colony forming units was compared to non-radiated controls. the effect of ultraviolet radiation differed among ba ...200011307403
influence of antimicrobial subinhibitory concentrations on hemolytic activity and bacteriocin-like substances in oral fusobacterium nucleatum.fusobacterium nucleatum is considered for its role in colonization of initial and late microorganisms in dental plaque and for its coaggregation with other bacterial species. it is known that action of different antimicrobial substances may interfere with either virulence factors or with host-bacteria interaction. the goal of this study was to examine the influence of subinhibitory concentrations of chlorhexidine, triclosan, penicillin g and metronidazole on hemolytic activity and bacteriocin-li ...200010872683
features of severe periodontal disease in a teenager with chédiak-higashi syndrome.chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis.200010872965
the periodontal abscess (i). clinical and microbiological findings.little information is available regarding the diagnosis and microbiology of periodontal abscesses. the aim of this descriptive clinical and microbiological study was to provide more information in order to help in the characterisation of the periodontal abscess associated to periodontitis.200010883867
early colonization of dental implants by putative periodontal pathogens in partially edentulous patients.there is limited scientific information available on the early colonization of the peri-implant pockets in partially edentulous individuals. knowledge about this process is one step in better understanding the etiology and pathogenesis of peri-implantitis. in this study, the early colonization of the peri-implant pockets by putative periodontal pathogens was studied in 20 partially edentulous individuals using anaerobic culture techniques. at baseline, the presence and levels of putative periodo ...200011168244
anaerobic bacterial extracts influence production of matrix metalloproteinases and their inhibitors by human dental pulp cells.the role of human dental pulp (hdp) cells in extracellular matrix degradation in pulpitis is still unclear. in this study, the effects of sonicated bacterial extracts (sbes) from prevotella intermedia, fusobacterium nucleatum, porphyromonas endodontalis, and porphyromonas gingivalis on the balance between the production of matrix metalloproteinases (mmps) and that of their inhibitors [tissue inhibitors of metalloproteinases (timps)] by hdp cells were examined. hdp cells were treated with sbes, a ...200011199767
assessment of periradicular microbiota by dna-dna hybridization.in the present study the "checkerboard" dna-dna hybridization technique was used to identify bacteria in periapical endodontic lesions of asymptomatic teeth. thirty-four patients with root-filled teeth and apical periodontitis were divided into two groups, each containing 17 patients. in group 1, a marginal incision was performed during surgery to expose the lesion, and in group 2, a submarginal incision was applied. the gingiva and mucosa were swabbed with an 0.2% chlorhexidine gluconate soluti ...200011202881
comparison of profiles of key periodontal pathogens in periodontium and endodontium.despite the established anatomical relationship between the periodontal and pulpal tissues, bacterial migration between endodontium and periodontium is still under discussion. the objective of this study was an investigation of profiles of periodontal pathogens in pulpal and periodontal diseases affecting the same tooth by means of 16s rrna gene directed polymerase chain reaction (pcr). 31 intact teeth with both pulp and marginal infections were investigated. the diagnosis was based on clinical ...200011202893
[bacterial virulence in the etiology of periodontal diseases].strong relationships have been very often described between various form of periodontal disease (pd) and certain bacterial species, so that nowadays periodontal disease is recognized as an infectious disease. destruction of periodontal supporting tissues happens as a response to very intricate host-parasite interactions. when the clinician will be able to fully understand and identify such phenomena it would be possible to succeed in a properly diagnosis and control of the active phase of period ...200011268937
effect of supragingival plaque control on subgingival microflora and gingivo-periodontal tissues.the aim of this study was to carry out a short term evaluation of the effect of supragingival plaque control on the subgingival microflora and gingivoperiodontal tissues in patients with moderate and severe periodontitis. a total of 92 sites from 23 patients were studied; patients ranged between 35 and 60 years of age (x 46.4 +/- 1.71). plaque index (pi), gingival index (gi), bleeding on probing (bp), probing depth (pd), and attachment level (al) were measured. subgingival microflora of the same ...200015211927
the results of prostatectomy: a symptomatic and urodynamic analysis of 152 patients.there were 152 patients with prostatism investigated by inflow cystometry and pressure flow analysis of micturition before and after elective prostatectomy. many symptoms attributed to obstruction were found to be owing to bladder instability, which was demonstrated in 60 per cent of the patients. the symptomatic improvement postoperatively was accompanied by a decrease in the incidence of instability to 25 per cent. the repeat urodynamic studies and symptom analysis demonstrated a surgical succ ...200086617
what is right with american medicine. 200097798
heroin epidemiology and the demand for heroin. 200097547
tissue cea in premalignant epithelial lesions and epidermoid carcinoma of the uterine cervix: prognostic significance.carcinoembryonic antigen (cea) was studied by the indirect triple-bridge immunoperoxidase method in formalin-fixed paraffin-embedded tissue specimens from 191 patients with premalignant epithelial lesions or epidermoid carcinoma of the uterine cervix treated 12 years ago. the frequency of tissue cea positivity was found to increase with advancing clinical disease in the following manner: mild dysplasia, 25%; severe dysplasia, 37%; carcinoma in situ, 60%; invasive carcinoma stage i, 60%; stage ii ...200086522
microbial oxidation of methane and methanol: isolation of methane-utilizing bacteria and characterization of a facultative methane-utilizing isolate.a methane-utilizing organism capable of growth both on methane and on more complex organic substrates as a sole source of carbon and energy, has been isolated and studied in detail. suspensions of methane-grown cells of this organism oxidized c-1 compounds (methane, methanol, formaldehyde, formate); hydrocarbons (ethane, propane); primary alcohols (ethanol, propanol); primary aldehydes (acetaldehyde, propionaldehyde); alkenes (ethylene, propylene); dimethylether; and organic acids (acetate, mala ...2000101517
an investigation into the antigen-specificity of transfer factor in its stimulatory action on lymphocyte transformation.dialysable transfer factor (tf) was prepared from the buffy-coat cells of donors with known cell-mediated reactivity to tuberculin (ppd), streptococcal protein (sksd) and diphtheria toxoid (dt). the effect of such preparations on the transformation by these antigens of lymphocytes from tuberculin-negative donors was investigated. transformation was determined as incorporation of tritiated thymidine. the concentrations of sksd and dt were adjusted for different lymphocyte donors so as to give, in ...200086502
biliary duct ultrasound. 200099016
clomipramine and clonazepam in cataplexy. 200091833
axonal transport of a brain-specific protein in the optic fibres of octopus vulgaris. 200090124
progression of diabetic nephropathy.although deterioration of renal function in diabetic nephropathy varies considerably from one diabetic to another, its rate is constant in individuals. for each patient there is a linear relation between period (months) which elapses from the time serum creatinine becomes greater than 200 mumol/l and the inverse the inverse of the serum-creatinine. the observation is of practical importance in predicting the time at which end-stage renal failure will develop, so that treatment can be planned in ...200086831
multiple ophthalmic anomalies and digital hypoplasia.multiple congenital eye and hand anomalies occurred in a young female born to normal but consanguineous parents. both eyes were microphthalmic with severe corneal, iris lens pathology. ultrasonography revealed multiple echos from the vitreous. the ocular findings are suggestive of retinal dysplasia. a skeletal dysplasia, presenting as distal phalangeal hypoplasia, was found in both hands. there was no history of intrauterine exposure to drugs. this appears to be a unique association of congenita ...200097363
[machine method of applying a sorbent layer to chromatographic plates]. 200090206
the effect of rectal examination on serum acid phosphatase. 200090146
the hazards of anesthesia and surgery in bleomycin-treated patients. 200188070
manometric study of the lower oesophageal sphincter in adult nigerians in relation to the rarity of hiatus hernia and symptomatic gastro-oesophageal reflux. 200197947
parietal association cortex in the primate: sensory mechanisms and behavioral modulations. 200198614
demonstration of the existence of small local circuit neurons in the golgi-stained primate substantia nigra. 200189001
external separation, internal ligation and injection in circumferential mixed hemorrhoids. analysis of 202 cases. 200198305
if i had 2500 million pounds to spend on cancer. 200190809
electroimmunoassay of factor ix in patients with liver damage and vitamin k unresponsive coagulation disorder.factor ix antigen and activity were assayed in a group of patients with liver disease and in a group of patients in coumarin therapy. in patients with liver disease there was a similar decrease in activity and antigen. on the other hand factor ix activity is decreased in coumarin treatment with factor ix antigen remaining normal. factor ix electrophoretic mobility in liver disease is normal.200188391
individual mice recognize the complex nature of h-2 antigens; unexpected reactions (anti-kk) in anti-balb/c-h-2d sera produced in the balb/c-h-2db mutant. 200188092
new consultant contract. 200187861
clinical significance of precipitating anti-rifampicin antibodies. 200198841
posttraumatic epilepsy and acute intermittent porphyria: effects of phenytoin, carbamazepine, and clonazepam.a patient with uncontrolled posttraumatic epilepsy and acute intermittent prophyria was subjected to successive therapeutic trials with phenytoin, carbamazepine, and clonazepam, while eating an adequate diet. both phenytoin and carbamazepine treatments caused significant increases in porphobilinogen excretion and appeared to induce acute porphyric attacks. in contrast, treatment with clonazepam under rigid dietary control for 10 days caused no increase in porphilbinogen excretion. during the sub ...200198734
effects of h-reflex conditioning upon the contralateral alpha motoneuron pool.h-reflex recovery curves have been elicited by subliminal conditioning stimuli applied to the ipsilateral and contralateral posterior tibial nerves in 10 healthy female subjects. in both types of recovery curve there was clear evidence of a period of facilitation in the ipsilateral soleus motoneuron pool 75-250 msec after the conditioning stimulus. these results indicate the bilateral nature of the facilitation and show it to be most probably produced by stimulus-evoked inputs as opposed to twit ...200188332
pleural herniation in respiratory distress syndrome. 200198029
induction of hard tissue into pulpless open-apex teeth using collagen-calcium phosphate gel. 200198608
haemodialysis in schizophrenia. 200192733
fetal heart-rate monitoring. 200190290
surgical wound sepsis. 200187737
[personal experience with the total hip prosthesis]. 20013739284
extramedullary haematopoiesis in thrombotic thrombocytopenic purpura.extramedullary hematopoiesis was detected in routine sections of spleen and liver from two patients with thrombotic thrombocytopenic purpura (ttp). in patients with other types of hemolytic anemia and in normal persons, extramedullary hematopoiesis was not found in spleen or liver. the results of this study raise the possibility that ttp may fit within the myeloproliferative category, perhaps representing an acute variant of di-guglielmo syndrome.200188388
igd myeloma protein with "unreactive" light chain determinants.serum from a patient with multiple myeloma showed a monoclonal protein, classified by immunoelectrophoresis as igd. immunofixation electrophoresis and immunoelectrophoresis failed to demonstrate a precipitation reaction between the paraprotein and antisera to immunoglobulin light chains. the light chains of the monoclonal protein, immunologically inaccessible in the intact molecule, reacted with anti-lambda chain antisera only after reduction and alkylation of the paraprotein. moreover, interpre ...200188283
angiolithiasis in the parotid gland.angiolithiasis in salivary glands is a very rare finding, and the knowledge of the morphology and histogenesis of these stone formations is therefore limited. six small angioliths from the left parotid gland of a 19-year-old girl were examined histologically, microradiographically and diffractometrically. the angiolithiasis developed from a regressed hemangioma which had been observed since the patient was 3--4 years old. the angioliths, which were encapsulated, exhibited in some areas diffuse l ...200198457
clinical significance of biologic markers: memorial hospital experience. 200188073
the results of randomised and other clinical trials of fast neutrons from the medical research council cyclotron, london. 200196057
analysis of 1089 cases of head injury.1089 patients treated for head injuries at the unversity of nigeria teaching hospital, enugu, are analysed. seventy-two percent of them were males. the age group 20-30 years was most vulnerable, accounting for 32% of the total. road traffic accidents were responsible for a staggering 80% of the total. most of the children in this group were pedestrians hit by vehicles on the roads. about 42% of the head injuries were complicated, but the incidence of intracranial haematoma was only 1.3%. the mor ...200197952
testing meningococcal vaccines for mitogenicity and superantigenicity.proteins with intrinsic mitogenic properties are widely represented in prokaryotes, such as in different streptococcus species (1-3), candida albicans (4), and eikenella corrodens (5). specifically, several bacterial porins of escherichia coli, shigella dysenteriae, salmonella typhimurium, fusobacterium nucleatum, and pathogenic neisseria species have been shown to induce nonspecific proliferation of lymphocytes (6-12).200121336757
serum phenytoin concentration and igg antibody titre to periodontal bacteria in patients with phenytoin-induced gingival overgrowth.epileptic patients taking phenytoin with gingival-overgrowth and those without gingival-overgrowth were compared for daily drug dose, plasma total phenytoin concentration, plasma free-phenytoin concentration and serum igg antibody titre against 13 periodontal bacteria. significantly higher daily drug dose was noted in patients with gingival overgrowth (p < 0.05) when compared with those without overgrowth. in addition, both total and free forms of plasma phenytoin concentration were significantl ...200112666977
validation of an in vitro biofilm model of supragingival plaque.the study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. we describe a model of supragingival plaque containing actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to al ...200111269730
nitric oxide production and inos mrna expression in mice induced by repeated stimulation with live fusobacterium nucleatum.there have been few studies on the detection of direct nitric oxide (no) production and interferon-gamma (ifn-gamma) in vivo without using animal cell culture. we questioned whether no and ifn-gamma could be produced at the site of infection. the peritoneal cavity of mice was used as the local infection model. no and ifn-gamma in abdominal washings from these mice were measured directly at various times after injection of fusobacterium nucleatum, a gram-negative rod periodontal pathogen. the mic ...200111270609
topological investigations of the foma porin from fusobacterium nucleatum and identification of the constriction loop l6.porin foma in the outer membrane of fusobacterium nucleatum is a trimeric protein, which exhibits permeability properties similar to that of the well-known enterobacterial diffusion porins. the proposed topology model of the foma monomer depicts the beta-barrel motif typical of diffusion porins, consisting of 16 antiparallel beta-strands. to investigate the accuracy of the foma model and assess the topological relationship with other porins, individual deletions of variable size in seven of the ...200111283301
smoking and subgingival microflora in periodontal disease.the present investigation was undertaken to analyze the influence of smoking on the periodontal disease associated subgingival microflora. the population included 33 smokers and 31 non-smokers in the age range 36-86 years.200111284533
periodontal pathogens produce quorum sensing signal molecules.different species of bacteria important in the composition of dental plaque were tested for production of extracellular autoinducer-like activities that stimulate the expression of the luminescence genes in vibrio harveyi. several strains of prevotella intermedia, fusobacterium nucleatum, and porphyromonas gingivalis were found to produce such activities. interestingly, these bacteria belong to the same phylogenetic group, and they are periodontal pathogens important in the development of period ...200111292769
activation of c-jun n-terminal kinase in the absence of nfkappab function prior to induction of nk cell death triggered by a combination of anti-class i and anti-cd16 antibodies.addition of antibodies to major histocompatibility complex class i (mhc class i) and f(c) gamma riii (cd16) antigens resulted in the synergistic augmentation of natural killer (nk) cell death, and the loss of nk cell cytotoxic function. the binding of anti-cd16 and anti-class i antibodies to the same population of nk cells is required for the synergistic augmentation of nk cell death. moreover, the addition of antibodies to leukocyte function antigen-1 (lfa-1), which significantly inhibited the ...200111295464
spatial arrangements and associative behavior of species in an in vitro oral biofilm model.the spatial arrangements and associative behavior of actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis strains in an in vitro model of supragingival plaque were determined. using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distribution of the five strains were assessed during biofilm formation. the volume-derived cell numbers of each strain correlated w ...200111229930
effect of lipopolysaccharide from periodontal pathogens on the production of tissue plasminogen activator and plasminogen activator inhibitor 2 by human gingival fibroblasts.both tissue plasminogen activator (t-pa) and plasminogen activator inhibitor 2 (pai-2) are important proteolysis factors present in inflamed human periodontal tissues. the aim of the present study was to investigate the effect of lipopolysaccharide (lps) on the synthesis of t-pa and pai-2 by human gingival fibroblasts (hgf). lps from different periodontal pathogens including actinobacillus actinomycetemcomitans, porphyromonas gingivalis and fusobacterium nucleatum were extracted by the hot pheno ...200111246701
fusobacterium nucleatum hepatic abscess with pylephlebitis associated with idiopathic cd4(+) t lymphocytopenia. 200111170936
interleukin-8 and intercellular adhesion molecule 1 regulation in oral epithelial cells by selected periodontal bacteria: multiple effects of porphyromonas gingivalis via antagonistic mechanisms.interaction of bacteria with mucosal surfaces can modulate the production of proinflammatory cytokines and adhesion molecules produced by epithelial cells. previously, we showed that expression of interleukin-8 (il-8) and intercellular adhesion molecule 1 (icam-1) by gingival epithelial cells increases following interaction with several putative periodontal pathogens. in contrast, expression of il-8 and icam-1 is reduced after porphyromonas gingivalis atcc 33277 challenge. in the present study, ...200111179300
the sensitivity of porphyromonas gingivalis and fusobacterium nucleatum to different (pseudo)halide-peroxidase combinations compared with mutans streptococci.previous studies have shown that the peroxidase system with iodide is particularly effective against actinobacillus actinomycetemcomitans. in the present study, the effects of iodide, chloride and thiocyanate in combinations with lactoperoxidase (lp) and myeloperoxidase (mp) on the viability of porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and s. rattus were analysed. bacteria were incubated in buffer solution containing peroxidase, substrate(s) and h2o2 (all in oral ph ...200111192504
antimicrobial activity of silver nitrate against periodontal pathogens.metal ions were evaluated as potential antimicrobial agents suitable for local delivery in the oral cavity for the treatment of periodontitis. silver nitrate, copper chloride, and zinc chloride were tested for antimicrobial activity in in vitro killing assays conducted in phosphate buffered saline with a series of oral bacteria including gram-negative periodontal pathogens and gram-positive streptococci. copper and zinc salts failed to exhibit strong and consistent activity against periodontal p ...200111327077
new assay for measuring cell surface hydrophobicities of candida dubliniensis and candida albicans.hydrophobic interactions, based on cell surface hydrophobicity (csh), are among the many and varied mechanisms of adherence deployed by the pathogenic yeast candida albicans. recently it was shown that, unlike c. albicans, c. dubliniensis is a species that exhibits an outer fibrillar layer consistent with constant csh. previously, c. dubliniensis grown at 25 or 37 degrees c was shown to coaggregate with the oral anaerobic bacterium fusobacterium nucleatum. c. albicans, however, demonstrated simi ...200111329462
heterogeneity of host immunological risk factors in patients with aggressive periodontitis.the pathogenesis of early-onset periodontitis (eop) can be explained by various host risk factors. previous studies have focused on a single (among many possible) immunological risk factor and the association among the factors has not been assessed. we comprehensively investigated the associations among multiple host immunological risk factors in eop patients to further elucidate their role in the pathogenesis of eop.200111338294
relationship of cigarette smoking to the subgingival microbiota.the relationship of cigarette smoking to the composition of the subgingival microbiota is not clear. some studies indicated higher levels of certain species in smokers, while other studies failed to detect differences in the microbiota between subjects with different smoking histories. thus, the purpose of the present investigation was to examine the prevalence, proportions and levels of the subgingival species in adult subjects who were current, past or never smokers.200111350499
lipid peroxidation caused by oxygen radicals from fusobacterium-stimulated neutrophils as a possible model for the emergence of periodontitis.the possible contribution of bacteria and polymorphonuclear neutrophils (pmn) to the disease process of periodontitis was evaluated.200111354921
kinetics of coaggregation of porphyromonas gingivalis with fusobacterium nucleatum using an automated microtiter plate assay.coaggregation between porphyromonas gingivalis and fusobacterium nucleatum strains was previously studied using either a semi-quantitative macroscopic assay or radioactive tracer assays. a new automated microtiter plate assay is introduced, in which the plate reader (vmax) was adapted to allow quantitative evaluation of the kinetics of coaggregation. f nucleatum pk 1594 coaggregated with p. gingivalis hg 405 with a maximal coaggregation rate of 1.05 mod/min, which occurred at a p. gingivalis to ...200111358538
microflora in oral ecosystems in primary sjögren's syndrome.knowledge of the effect of primary sjögren's syndrome (pss) on the microbial flora in the different predilection sites for oral disorders is needed for planning preventive treatment. we carried out microbial analysis of samples from the dorsum of the tongue, smooth mucosa, supragingival tooth surfaces, and the gingival crevice region of 20 patients with pss.200111361180
septic arthritis of the hip due to fusobacterium nucleatum.anaerobic bacteria are uncommon pathogens in septic arthritis. we report a case of pyarthrosis of the right hip caused by fusobacterium nucleatum, following a transient synovitis in an otherwise healthy boy. there are only a few cases involving this species described in the literature. this report illustrates the difficulty of isolating this unusual organism and emphasises the usefulness of the bactec blood culture bottles for the recovery of anaerobic bacteria.200111434480
risk factors for anaerobic bloodstream infections in bone marrow transplant recipients.the incidence of anaerobic bloodstream infections (bsi) in patients who underwent bone marrow transplantation (bmt) recently increased at our institution. a retrospective case-control study of patients undergoing bmt from january 1995 through december 1998 was performed to determine the microbiological characteristics, epidemiology, and outcome of anaerobic bsi and to identify independent risk factors for infection. anaerobic bsi occurred in 23 patients, for a rate of 4 bsis per 100 bmt procedur ...200111438899
antimicrobial effect of acidified nitrite on periodontal bacteria.the antimicrobial agent nitric oxide (no) is formed in the mouth and its concentration is directly related to salivary nitrite, which in turn is related to dietary nitrate intake. the aim of this study was to determine whether nitrite under acidic conditions will have an inhibitory effect, possibly occurring through no production, on the periodontal disease pathogens fusobacterium nucleatum, eikenella corrodens and porphyromonas gingivalis. whereas the growth of these organisms was inhibited by ...200111442852
evaluation of co-aggregation among streptococcus mitis, fusobacterium nucleatum and porphyromonas gingivalis.to develop a semi-quantitative method for evaluating co-aggregation reactions among three bacterial species, and to examine the influence of fusobacterium nucleatum on the adherence of porphyromonas gingivalis.200111472519
an in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.the bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.200111482142
antibacterial properties of electron beam-sterilized gutta-percha cones.the purpose of the study was to determine the effect of electron beam sterilization on gutta-percha cones (gpcs) at different times after sterilization. an agar diffusion test was used with -one aerobic bacterium (bacillus subtilis) and five oral anaerobic bacteria (fusobacterium nucleatum, peptostreptococcus micros, porphyromonas gingivalis, propionibacterium acnes, and veillonella parvula). with each microorganism 30 agar plates were prepared, evenly distributed among three groups (group 1: un ...200111487145
metallic cough and pyogenic liver abscess.the curious symptom of a metallic cough in association with a pyogenic hepatic abscess should heighten awareness of a fistula. we describe a 78-year-old female with severe diverticular disease, on long-term steroid treatment for polymyalgia rheumatica. she developed a pyogenic liver abscess, treated initially by antimicrobial therapy, and subsequently drained by ultrasound and computed tomography-guided percutaneous transhepatic pigtail catheterization. this was complicated by a fistulous commun ...200111507364
antibacterial activity of particulate bioglass against supra- and subgingival bacteria.particulate bioglass is a bioactive material used in the repair of periodontal defects. this material undergoes a series of surface reactions in an aqueous environment which lead to osseointegration. the aim of this study was to determine whether these reactions exerted an antibacterial effect on a range of oral bacteria. streptococcus sanguis, streptococcus mutans and actinomyces viscosus were suspended in nutrient broth (nb), artificial saliva (as) or dulbecco's modified eagle medium plus 10% ...200111374470
smoking affects the subgingival microflora in periodontitis.tobacco smoking has been identified as one major risk factor for destructive periodontal disease. scaling and root planing have been shown to be less effective in smokers with periodontitis. the aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers.200111394403
change in subgingival microbial profiles in adult periodontitis subjects receiving either systemically-administered amoxicillin or metronidazole.the current investigation evaluated changes in levels and proportions of 40 bacterial species in subgingival plaque samples during, immediately after and up to 1 year after metronidazole or amoxicillin therapy combined with srp.200111422580
untreated periodontal disease in indonesian adolescents. subgingival microbiota in relation to experienced progression of periodontitis.in an indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. at baseline the age ranged from 15 to 25 years. clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up.200111422582
mutans streptococci in subgingival plaque of treated and untreated patients with periodontitis.the etiology of root caries is thought to be identical to coronal caries, though root caries seem to be more complicated because of the higher susceptibility of exposed roots (dentin) by periodontal therapy to demineralization than intact enamel. this implies that mutans streptococci are the most likely pathogens in the development of root caries. although it is known that both the numbers of mutans streptococci and the frequency of isolation in root caries lesions are negatively correlated with ...200111422591
associations between serum antibody levels to periodontal pathogens and early-onset periodontitis.the role of antibodies to periodontal microorganisms in the development of periodontal tissue destruction is still unclear. the aim of this study was to investigate the association between serum levels of igg, iga, and igm antibodies to 6 periodontal microorganisms and clinical subtypes of varying severity of early-onset periodontitis (eop) in young african american adults.200111759856
determination of diamino acids in peptidoglycans from anaerobic bacteria.peptidoglycans isolated from two fusobacterium species of anaerobic bacteria were analyzed for constituent amino acids. hydrolysis conditions were varied to optimize the yield of diamino acids from peptidoglycan. the o-phthalaldehyde derivatives of the diamino acid stereoisomers were separated by high-performance liquid chromatography (opa-hplc), and variations in the relative areas of the two peaks noticed during analysis of solid samples were attributed to sampling errors. co-derivatization/in ...200111764403
the heat shock response of fusobacterium nucleatum.the heat-shock response of the oral gram-negative bacterium fusobacterium nucleatum was examined. different strains of f. nucleatum were grown at 37 c. 42 degrees c and 48 c in the presence of [35s]methionine. cellular proteins synthesised after shifts to higher temperatures were analysed by sds-page and autoradiography. strains atcc 10953, f1, f3 and fev1 exhibited heat-shock response, and major proteins were observed at 60, 70 and 90 kda. but increased protein synthesis was also observed for o ...200111767277
[septic shock with liver abscess in an immunocompetence patient. presentation of an unusual fusobacterium nucleatum infection].fusobacterium septicemia with septic metastasis is a very rare and potentially serous condition when complicated by septic shock. most cases have been described in patients with humoral immunity disorders and/or severe underlying chronic disease.200111771203
antimicrobial activity of four root canal sealers against endodontic pathogens.the antibacterial effects of various types of widely used endodontic sealers have not been compared systematically on facultative or obligate anaerobic endodontic pathogens. the aim of this study was to evaluate the antimicrobial properties of four commonly used endodontic sealers: two epoxy-resin-based sealers (ah26, ah plus), one zinc-oxide eugenol-based sealer (n2), and one calcium hydroxide-based sealer (sealapex). the testing microbes were four facultative anaerobic species (streptococcus m ...200111800436
implantation of bacteria from human pulpal necrosis and translocation from root canals in gnotobiotic mice.the aim of this study was to determine whether microorganisms recovered from infected human root canals were able to survive and translocate to a local lymph node when experimentally inoculated into the root canal system of germ-free mice. the microorganisms isolated from two patients with pulpal necrosis were inoculated in two groups of experimental animals; group i (gemella morbillorum) and group ii (bifidobacterium adolescentis, fusobacterium nucleatum, and clostridium butyricum). g. morbillo ...200111592487
[clinical and microbiological study of adult periodontal disease].the aim of this study was to carry out a microbiological evaluation of sites with and without clinical evidence of moderate and severe periodontitis and their correlation with clinical parameters. a total of 52 disease sites and 10 healthy sites were selected according to clinical criteria. the following clinical indexes were measured for all the sites: plaque index, gingival index, blood on probing, depth on probing and insertion level. samples of subgingival plaque were collected for culture a ...200111594003
phosphorylcholine-dependent cross-reactivity between dental plaque bacteria and oxidized low-density lipoproteins.antibodies reactive with phosphorylcholine (pc) are ubiquitous in human sera, but the antigens stimulating their production and their function are not clear. previous studies have shown that a significant proportion of dental plaque bacteria contain pc as determined by reactivity with pc-specific mouse myeloma proteins and monoclonal antibodies. additionally, serum antibody concentrations of immunoglobulin (igg) g anti-pc are higher in sera of individuals who have experienced periodontal attachm ...200111598029
microbiological evaluation of acute periradicular abscesses by dna-dna hybridization.the purpose of the present investigation was to examine the microbiota of acute periradicular abscesses of endodontic origin by using a molecular genetic method.200111598583
susceptibilities of oral bacteria and yeast to mammalian cathelicidins.the effects of cathelicidins against oral bacteria and clinically important oral yeasts are not known. we tested the susceptibilities of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis, candida krusei, candida tropicalis and candida albicans to the following cathelicidins: fall39, smap29, and cap18. smap29 and cap18 were antimicrobial, whereas fall39 did not exhibit antimicrobial activity. future studies are needed to determine the p ...200111600383
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