Publications

the fusobacterium nucleatum porin foma possesses the general topology of the non-specific porins.	foma is a major non-specific porin of fusobacterium nucleatum with no sequence similarity to other known porins. according to the topology model, the protein consists of 16 transmembrane beta-strands, connected by eight surface-exposed loops and seven periplasmic turns. in this study, the insertion mutagenesis approach was applied to probe the topology model. a semliki forest virus (sfv) epitope was successfully inserted at 11 different sites of the foma protein and a 6-aa insertion was successf ...	2000	10846222
lipopolysaccharide induces expression of genes encoding pro-inflammatory cytokines and the elastin-degrading enzyme, cathepsin s, in human cervical smooth-muscle cells.	vaginal and amniotic infection with gram-negative bacteria is associated with preterm birth. we previously reported that human cervical smooth-muscle cells (csmc) respond to pro-inflammatory cytokines by expressing enzymes that degrade the extracellular matrix. our objective was to characterize the effects of lipopolysaccharide (lps) from escherichia coli (e coli), bacteroides fragilis, (b frag)and fusobacterium nucleatum (f nuc)on the expression of pro-inflammatory cytokines and the elastin-deg ...	2000	10865188
effects of streptococcus crista and human saliva on the viability of fusobacterium nucleatum atcc 10953.	associations with facultative species might help planktonic oral anaerobes survive when they traverse saliva. this study investigated whether co-aggregation with streptococcus crista atcc 51110 enhanced the viability of fusobacterium nucleatum atcc 10953. two tubes each of co-aggregates and fus. nucleatum atcc 10953 alone were prepared. one of each set was resuspended in buffer, and the other in clarified saliva from each of 20 donors. after 1 h, cells were stained for viability. the median perc ...	2000	10869478
assessment of periradicular microbiota by dna-dna hybridization.	in the present study the "checkerboard" dna-dna hybridization technique was used to identify bacteria in periapical endodontic lesions of asymptomatic teeth. thirty-four patients with root-filled teeth and apical periodontitis were divided into two groups, each containing 17 patients. in group 1, a marginal incision was performed during surgery to expose the lesion, and in group 2, a submarginal incision was applied. the gingiva and mucosa were swabbed with an 0.2% chlorhexidine gluconate soluti ...	2000	11202881
laboratory diagnosis of anaerobic pleuropulmonary infections.	anaerobic organisms play a major role in pleuropulmonary infections. clinical manifestations range from simple aspiration to acute, severe, necrotizing pneumonias to chronic infections, such as lung abscess and empyema. implicated pathogens are usually of endogenous origin. laboratory diagnosis of anaerobic pleuropulmonary infections is based on recovering the etiological agent from clinical specimens. appropriate specimens include pleuml fluid, transtracheal aspirates, transthoracic aspirates a ...	2000	10983929
the virulence of mixed infection with streptococcus constellatus and fusobacterium nucleatum in a murine orofacial infection model.	orofacial infections are usually polymicrobial, and it is the microbial interactions of pathogenic species that cause tissue destruction. in this study, the microbial interaction between streptococcus constellatus and fusobacterium nucleatum was characterized using a murine orofacial infection model. a mixture of viable s. constellatus and f. nucleatum cells (both 2 x 10(8) cfu/mouse) was injected into the submandible; as a result, all of the test mice died. in contrast, none of the experimental ...	2000	11099928
b-cell deficiency predisposes mice to disseminating anaerobic infections: protection by passive antibody transfer.	we have previously demonstrated that a high proportion of rag-2 scid knockout mice, which lack t and b cells, develop orofacial abscesses and disseminated infections following pulpal infection, whereas immunocompetent control mice do not. in the present study, we sought to identify the components of the adaptive immune response which contribute to protection against disseminating anaerobic infections and sepsis. for this purpose, various genetically engineered immunodeficient mice were employed, ...	2000	10992465
[analysis using elisa test of antibody response to fusobacterium nucleatum and eikenella corrodens in subjects with periodontal disease].	to know the synthesis of igg, iga, and igm to fusobacterium nucleatum and eikenella corrodens in serum, crevicular liquid and saliva in subjects with periodontal disease, using elisa test.	2000	10996873
detection of major putative periodontopathogens in korean advanced adult periodontitis patients using a nucleic acid-based approach.	although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a korean population so far. the purpose of this study was to describe the prevalence of major putative periodontopathogens in korean patients by culture-independent methods.	2000	11022767
cytokine, elastase and oxygen radical release by fusobacterium nucleatum-activated leukocytes: a possible pathogenic factor in periodontitis.	periodontitis is characterised by tissue destruction caused by reactive oxygen species (ros) and proteolytic enzymes, which are released by the interaction between bacteria and phagocytes. we estimated the ability of fusobacterium species to induce release of tissue destructive and proinflammatory mediators from in vitro activated peripheral leukocytes. ros was measured with the nitroblue tetrazolium (nbt) method, elastase with a specific chromogenic substrate and cytokines, including interleuki ...	2000	11034124
profile of subgingival microbiota in children with mixed dentition.	a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...	2000	11155173
differences in the composition of the subgingival microbiota of two periodontitis populations of different geographical origin. a comparison between spain and the netherlands.	the purpose of this study was to compare the subgingival microbiota of two geographically distinct patient populations using identical clinical and bacteriological methods. adult patients with a diagnosis of periodontitis were consecutively selected according to pre-defined clinical criteria. microbiological samples were taken from the deepest four sites with bleeding. the samples were plated on blood agar plates, for the determination of the total anaerobic counts and identification of specific ...	2000	11037754
the response to oxidative stress of fusobacterium nucleatum grown in continuous culture.	fusobacterium nucleatum atcc 10953 was grown in continuous culture and the atmosphere changed stepwise from nitrogen (anaerobiosis) to a mixture of air: oxygen (40:60). no significant differences in biomass were observed and the baseline low level of superoxide dismutase increased only slightly; catalase and peroxidase activities were never detected but the level of nadh oxidase increased more than three-fold when oxygen was introduced into the system. in relation to acidic end-products, the rel ...	2000	10828396
[effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide].	to elucidate the effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide (lps).	2000	11780236
[antimicrobial effects of tea tree oil (melaleuca alternifolia) on oral microorganisms].	the essential oil of melaleuca alternifolia (tea tree oil) exhibits antimicrobial activity against a wide range of gram-positive and gram-negative bacteria, yeasts and fungi. in this study the bacteriostatic and bacteriocidal/fungicidal activity of a tea tree oil solution, of a new tea tree oil (tebodont) and the respective placebo-gel, of a chlorhexidindigluconate-solution and of plakout was tested in vitro against ten different oral microorganisms. minimum inhibitory concentrations were in the ...	2000	11374358
early colonization of dental implants by putative periodontal pathogens in partially edentulous patients.	there is limited scientific information available on the early colonization of the peri-implant pockets in partially edentulous individuals. knowledge about this process is one step in better understanding the etiology and pathogenesis of peri-implantitis. in this study, the early colonization of the peri-implant pockets by putative periodontal pathogens was studied in 20 partially edentulous individuals using anaerobic culture techniques. at baseline, the presence and levels of putative periodo ...	2000	11168244
anaerobic bacterial extracts influence production of matrix metalloproteinases and their inhibitors by human dental pulp cells.	the role of human dental pulp (hdp) cells in extracellular matrix degradation in pulpitis is still unclear. in this study, the effects of sonicated bacterial extracts (sbes) from prevotella intermedia, fusobacterium nucleatum, porphyromonas endodontalis, and porphyromonas gingivalis on the balance between the production of matrix metalloproteinases (mmps) and that of their inhibitors [tissue inhibitors of metalloproteinases (timps)] by hdp cells were examined. hdp cells were treated with sbes, a ...	2000	11199767
comparison of profiles of key periodontal pathogens in periodontium and endodontium.	despite the established anatomical relationship between the periodontal and pulpal tissues, bacterial migration between endodontium and periodontium is still under discussion. the objective of this study was an investigation of profiles of periodontal pathogens in pulpal and periodontal diseases affecting the same tooth by means of 16s rrna gene directed polymerase chain reaction (pcr). 31 intact teeth with both pulp and marginal infections were investigated. the diagnosis was based on clinical ...	2000	11202893
[bacterial virulence in the etiology of periodontal diseases].	strong relationships have been very often described between various form of periodontal disease (pd) and certain bacterial species, so that nowadays periodontal disease is recognized as an infectious disease. destruction of periodontal supporting tissues happens as a response to very intricate host-parasite interactions. when the clinician will be able to fully understand and identify such phenomena it would be possible to succeed in a properly diagnosis and control of the active phase of period ...	2000	11268937
disinfection of dental impressions and occlusal records by ultraviolet radiation.	as chemical disinfection of dental impressions may cause adverse effects on materials and the dental personnel this study examined disinfection by ultraviolet radiation. alginate, addition silicone rubber and red wax contaminated by streptococcus salivarius, fusobacterium nucleatum and five other bacteria in different suspension media were radiated for up to 18 min, and the number of colony forming units was compared to non-radiated controls. the effect of ultraviolet radiation differed among ba ...	2000	11307403
effect of supragingival plaque control on subgingival microflora and gingivo-periodontal tissues.	the aim of this study was to carry out a short term evaluation of the effect of supragingival plaque control on the subgingival microflora and gingivoperiodontal tissues in patients with moderate and severe periodontitis. a total of 92 sites from 23 patients were studied; patients ranged between 35 and 60 years of age (x 46.4 +/- 1.71). plaque index (pi), gingival index (gi), bleeding on probing (bp), probing depth (pd), and attachment level (al) were measured. subgingival microflora of the same ...	2000	15211927
[machine method of applying a sorbent layer to chromatographic plates].		2000	90206
what is right with american medicine.		2000	97798
the effect of rectal examination on serum acid phosphatase.		2000	90146
axonal transport of a brain-specific protein in the optic fibres of octopus vulgaris.		2000	90124
progression of diabetic nephropathy.	although deterioration of renal function in diabetic nephropathy varies considerably from one diabetic to another, its rate is constant in individuals. for each patient there is a linear relation between period (months) which elapses from the time serum creatinine becomes greater than 200 mumol/l and the inverse the inverse of the serum-creatinine. the observation is of practical importance in predicting the time at which end-stage renal failure will develop, so that treatment can be planned in ...	2000	86831
multiple ophthalmic anomalies and digital hypoplasia.	multiple congenital eye and hand anomalies occurred in a young female born to normal but consanguineous parents. both eyes were microphthalmic with severe corneal, iris lens pathology. ultrasonography revealed multiple echos from the vitreous. the ocular findings are suggestive of retinal dysplasia. a skeletal dysplasia, presenting as distal phalangeal hypoplasia, was found in both hands. there was no history of intrauterine exposure to drugs. this appears to be a unique association of congenita ...	2000	97363
the results of prostatectomy: a symptomatic and urodynamic analysis of 152 patients.	there were 152 patients with prostatism investigated by inflow cystometry and pressure flow analysis of micturition before and after elective prostatectomy. many symptoms attributed to obstruction were found to be owing to bladder instability, which was demonstrated in 60 per cent of the patients. the symptomatic improvement postoperatively was accompanied by a decrease in the incidence of instability to 25 per cent. the repeat urodynamic studies and symptom analysis demonstrated a surgical succ ...	2000	86617
heroin epidemiology and the demand for heroin.		2000	97547
microbial oxidation of methane and methanol: isolation of methane-utilizing bacteria and characterization of a facultative methane-utilizing isolate.	a methane-utilizing organism capable of growth both on methane and on more complex organic substrates as a sole source of carbon and energy, has been isolated and studied in detail. suspensions of methane-grown cells of this organism oxidized c-1 compounds (methane, methanol, formaldehyde, formate); hydrocarbons (ethane, propane); primary alcohols (ethanol, propanol); primary aldehydes (acetaldehyde, propionaldehyde); alkenes (ethylene, propylene); dimethylether; and organic acids (acetate, mala ...	2000	101517
an investigation into the antigen-specificity of transfer factor in its stimulatory action on lymphocyte transformation.	dialysable transfer factor (tf) was prepared from the buffy-coat cells of donors with known cell-mediated reactivity to tuberculin (ppd), streptococcal protein (sksd) and diphtheria toxoid (dt). the effect of such preparations on the transformation by these antigens of lymphocytes from tuberculin-negative donors was investigated. transformation was determined as incorporation of tritiated thymidine. the concentrations of sksd and dt were adjusted for different lymphocyte donors so as to give, in ...	2000	86502
biliary duct ultrasound.		2000	99016
clomipramine and clonazepam in cataplexy.		2000	91833
tissue cea in premalignant epithelial lesions and epidermoid carcinoma of the uterine cervix: prognostic significance.	carcinoembryonic antigen (cea) was studied by the indirect triple-bridge immunoperoxidase method in formalin-fixed paraffin-embedded tissue specimens from 191 patients with premalignant epithelial lesions or epidermoid carcinoma of the uterine cervix treated 12 years ago. the frequency of tissue cea positivity was found to increase with advancing clinical disease in the following manner: mild dysplasia, 25%; severe dysplasia, 37%; carcinoma in situ, 60%; invasive carcinoma stage i, 60%; stage ii ...	2000	86522
new consultant contract.		2001	87861
clinical significance of precipitating anti-rifampicin antibodies.		2001	98841
posttraumatic epilepsy and acute intermittent porphyria: effects of phenytoin, carbamazepine, and clonazepam.	a patient with uncontrolled posttraumatic epilepsy and acute intermittent prophyria was subjected to successive therapeutic trials with phenytoin, carbamazepine, and clonazepam, while eating an adequate diet. both phenytoin and carbamazepine treatments caused significant increases in porphobilinogen excretion and appeared to induce acute porphyric attacks. in contrast, treatment with clonazepam under rigid dietary control for 10 days caused no increase in porphilbinogen excretion. during the sub ...	2001	98734
effects of h-reflex conditioning upon the contralateral alpha motoneuron pool.	h-reflex recovery curves have been elicited by subliminal conditioning stimuli applied to the ipsilateral and contralateral posterior tibial nerves in 10 healthy female subjects. in both types of recovery curve there was clear evidence of a period of facilitation in the ipsilateral soleus motoneuron pool 75-250 msec after the conditioning stimulus. these results indicate the bilateral nature of the facilitation and show it to be most probably produced by stimulus-evoked inputs as opposed to twit ...	2001	88332
pleural herniation in respiratory distress syndrome.		2001	98029
induction of hard tissue into pulpless open-apex teeth using collagen-calcium phosphate gel.		2001	98608
demonstration of the existence of small local circuit neurons in the golgi-stained primate substantia nigra.		2001	89001
external separation, internal ligation and injection in circumferential mixed hemorrhoids. analysis of 202 cases.		2001	98305
if i had 2500 million pounds to spend on cancer.		2001	90809
electroimmunoassay of factor ix in patients with liver damage and vitamin k unresponsive coagulation disorder.	factor ix antigen and activity were assayed in a group of patients with liver disease and in a group of patients in coumarin therapy. in patients with liver disease there was a similar decrease in activity and antigen. on the other hand factor ix activity is decreased in coumarin treatment with factor ix antigen remaining normal. factor ix electrophoretic mobility in liver disease is normal.	2001	88391
individual mice recognize the complex nature of h-2 antigens; unexpected reactions (anti-kk) in anti-balb/c-h-2d sera produced in the balb/c-h-2db mutant.		2001	88092
[personal experience with the total hip prosthesis].		2001	3739284
extramedullary haematopoiesis in thrombotic thrombocytopenic purpura.	extramedullary hematopoiesis was detected in routine sections of spleen and liver from two patients with thrombotic thrombocytopenic purpura (ttp). in patients with other types of hemolytic anemia and in normal persons, extramedullary hematopoiesis was not found in spleen or liver. the results of this study raise the possibility that ttp may fit within the myeloproliferative category, perhaps representing an acute variant of di-guglielmo syndrome.	2001	88388
haemodialysis in schizophrenia.		2001	92733
surgical wound sepsis.		2001	87737
the hazards of anesthesia and surgery in bleomycin-treated patients.		2001	88070
igd myeloma protein with "unreactive" light chain determinants.	serum from a patient with multiple myeloma showed a monoclonal protein, classified by immunoelectrophoresis as igd. immunofixation electrophoresis and immunoelectrophoresis failed to demonstrate a precipitation reaction between the paraprotein and antisera to immunoglobulin light chains. the light chains of the monoclonal protein, immunologically inaccessible in the intact molecule, reacted with anti-lambda chain antisera only after reduction and alkylation of the paraprotein. moreover, interpre ...	2001	88283
fetal heart-rate monitoring.		2001	90290
angiolithiasis in the parotid gland.	angiolithiasis in salivary glands is a very rare finding, and the knowledge of the morphology and histogenesis of these stone formations is therefore limited. six small angioliths from the left parotid gland of a 19-year-old girl were examined histologically, microradiographically and diffractometrically. the angiolithiasis developed from a regressed hemangioma which had been observed since the patient was 3--4 years old. the angioliths, which were encapsulated, exhibited in some areas diffuse l ...	2001	98457
parietal association cortex in the primate: sensory mechanisms and behavioral modulations.		2001	98614
manometric study of the lower oesophageal sphincter in adult nigerians in relation to the rarity of hiatus hernia and symptomatic gastro-oesophageal reflux.		2001	97947
clinical significance of biologic markers: memorial hospital experience.		2001	88073
the results of randomised and other clinical trials of fast neutrons from the medical research council cyclotron, london.		2001	96057
analysis of 1089 cases of head injury.	1089 patients treated for head injuries at the unversity of nigeria teaching hospital, enugu, are analysed. seventy-two percent of them were males. the age group 20-30 years was most vulnerable, accounting for 32% of the total. road traffic accidents were responsible for a staggering 80% of the total. most of the children in this group were pedestrians hit by vehicles on the roads. about 42% of the head injuries were complicated, but the incidence of intracranial haematoma was only 1.3%. the mor ...	2001	97952
testing meningococcal vaccines for mitogenicity and superantigenicity.	proteins with intrinsic mitogenic properties are widely represented in prokaryotes, such as in different streptococcus species (1-3), candida albicans (4), and eikenella corrodens (5). specifically, several bacterial porins of escherichia coli, shigella dysenteriae, salmonella typhimurium, fusobacterium nucleatum, and pathogenic neisseria species have been shown to induce nonspecific proliferation of lymphocytes (6-12).	2001	21336757
antimicrobial activity of silver nitrate against periodontal pathogens.	metal ions were evaluated as potential antimicrobial agents suitable for local delivery in the oral cavity for the treatment of periodontitis. silver nitrate, copper chloride, and zinc chloride were tested for antimicrobial activity in in vitro killing assays conducted in phosphate buffered saline with a series of oral bacteria including gram-negative periodontal pathogens and gram-positive streptococci. copper and zinc salts failed to exhibit strong and consistent activity against periodontal p ...	2001	11327077
intra- and interspecies regulation of gene expression by actinobacillus actinomycetemcomitans luxs.	the cell density-dependent control of gene expression is employed by many bacteria for regulating a variety of physiological functions, including the generation of bioluminescence, sporulation, formation of biofilms, and the expression of virulence factors. although periodontal organisms do not appear to secrete acyl-homoserine lactone signals, several species, e.g., porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum, have recently been shown to secrete a signal related ...	2001	11705942
heterogeneity of host immunological risk factors in patients with aggressive periodontitis.	the pathogenesis of early-onset periodontitis (eop) can be explained by various host risk factors. previous studies have focused on a single (among many possible) immunological risk factor and the association among the factors has not been assessed. we comprehensively investigated the associations among multiple host immunological risk factors in eop patients to further elucidate their role in the pathogenesis of eop.	2001	11338294
relationship of cigarette smoking to the subgingival microbiota.	the relationship of cigarette smoking to the composition of the subgingival microbiota is not clear. some studies indicated higher levels of certain species in smokers, while other studies failed to detect differences in the microbiota between subjects with different smoking histories. thus, the purpose of the present investigation was to examine the prevalence, proportions and levels of the subgingival species in adult subjects who were current, past or never smokers.	2001	11350499
prior exposure of mice to fusobacterium nucleatum modulates host response to porphyromonas gingivalis.	multiple periodontal pathogens sequentially colonize the subgingival niche during the conversion from gingivitis to destructive periodontal disease. an animal model of sequential immunization with key periodontal pathogens has been developed to determine whether t- and b-lymphocyte effector functions are skewed and fail to protect the host from pathogenic challenge. the present study was performed to evaluate the immunomodulatory effect of exposure to fusobacterium nucleatum prior to porphyromon ...	2001	11737656
lactoferrin, amylase and mucin muc5b and their relation to the oral microflora in hyposalivation of different origins.	there are several reasons for hyposalivation, each affecting the salivary composition in different ways. the aim of this study was to analyze and compare lactoferrin, amylase and mucin muc5b in stimulated whole saliva collected from subjects with hyposalivation of different origins and to relate the results to the presence of some microbial species associated with oral disorders. albumin was determined as a marker of serum leakage. the characteristic feature for subjects with radiation-induced h ...	2001	11737657
lipid peroxidation caused by oxygen radicals from fusobacterium-stimulated neutrophils as a possible model for the emergence of periodontitis.	the possible contribution of bacteria and polymorphonuclear neutrophils (pmn) to the disease process of periodontitis was evaluated.	2001	11354921
validation of an in vitro biofilm model of supragingival plaque.	the study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. we describe a model of supragingival plaque containing actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to al ...	2001	11269730
nitric oxide production and inos mrna expression in mice induced by repeated stimulation with live fusobacterium nucleatum.	there have been few studies on the detection of direct nitric oxide (no) production and interferon-gamma (ifn-gamma) in vivo without using animal cell culture. we questioned whether no and ifn-gamma could be produced at the site of infection. the peritoneal cavity of mice was used as the local infection model. no and ifn-gamma in abdominal washings from these mice were measured directly at various times after injection of fusobacterium nucleatum, a gram-negative rod periodontal pathogen. the mic ...	2001	11270609
implantation of bacteria from human pulpal necrosis and translocation from root canals in gnotobiotic mice.	the aim of this study was to determine whether microorganisms recovered from infected human root canals were able to survive and translocate to a local lymph node when experimentally inoculated into the root canal system of germ-free mice. the microorganisms isolated from two patients with pulpal necrosis were inoculated in two groups of experimental animals; group i (gemella morbillorum) and group ii (bifidobacterium adolescentis, fusobacterium nucleatum, and clostridium butyricum). g. morbillo ...	2001	11592487
topological investigations of the foma porin from fusobacterium nucleatum and identification of the constriction loop l6.	porin foma in the outer membrane of fusobacterium nucleatum is a trimeric protein, which exhibits permeability properties similar to that of the well-known enterobacterial diffusion porins. the proposed topology model of the foma monomer depicts the beta-barrel motif typical of diffusion porins, consisting of 16 antiparallel beta-strands. to investigate the accuracy of the foma model and assess the topological relationship with other porins, individual deletions of variable size in seven of the ...	2001	11283301
smoking and subgingival microflora in periodontal disease.	the present investigation was undertaken to analyze the influence of smoking on the periodontal disease associated subgingival microflora. the population included 33 smokers and 31 non-smokers in the age range 36-86 years.	2001	11284533
periodontal pathogens produce quorum sensing signal molecules.	different species of bacteria important in the composition of dental plaque were tested for production of extracellular autoinducer-like activities that stimulate the expression of the luminescence genes in vibrio harveyi. several strains of prevotella intermedia, fusobacterium nucleatum, and porphyromonas gingivalis were found to produce such activities. interestingly, these bacteria belong to the same phylogenetic group, and they are periodontal pathogens important in the development of period ...	2001	11292769
susceptibilities of oral bacteria and yeast to mammalian cathelicidins.	the effects of cathelicidins against oral bacteria and clinically important oral yeasts are not known. we tested the susceptibilities of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis, candida krusei, candida tropicalis and candida albicans to the following cathelicidins: fall39, smap29, and cap18. smap29 and cap18 were antimicrobial, whereas fall39 did not exhibit antimicrobial activity. future studies are needed to determine the p ...	2001	11600383
activation of c-jun n-terminal kinase in the absence of nfkappab function prior to induction of nk cell death triggered by a combination of anti-class i and anti-cd16 antibodies.	addition of antibodies to major histocompatibility complex class i (mhc class i) and f(c) gamma riii (cd16) antigens resulted in the synergistic augmentation of natural killer (nk) cell death, and the loss of nk cell cytotoxic function. the binding of anti-cd16 and anti-class i antibodies to the same population of nk cells is required for the synergistic augmentation of nk cell death. moreover, the addition of antibodies to leukocyte function antigen-1 (lfa-1), which significantly inhibited the ...	2001	11295464
interleukin-8 and intercellular adhesion molecule 1 regulation in oral epithelial cells by selected periodontal bacteria: multiple effects of porphyromonas gingivalis via antagonistic mechanisms.	interaction of bacteria with mucosal surfaces can modulate the production of proinflammatory cytokines and adhesion molecules produced by epithelial cells. previously, we showed that expression of interleukin-8 (il-8) and intercellular adhesion molecule 1 (icam-1) by gingival epithelial cells increases following interaction with several putative periodontal pathogens. in contrast, expression of il-8 and icam-1 is reduced after porphyromonas gingivalis atcc 33277 challenge. in the present study, ...	2001	11179300
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.	the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...	2001	11120995
kinetics of coaggregation of porphyromonas gingivalis with fusobacterium nucleatum using an automated microtiter plate assay.	coaggregation between porphyromonas gingivalis and fusobacterium nucleatum strains was previously studied using either a semi-quantitative macroscopic assay or radioactive tracer assays. a new automated microtiter plate assay is introduced, in which the plate reader (vmax) was adapted to allow quantitative evaluation of the kinetics of coaggregation. f nucleatum pk 1594 coaggregated with p. gingivalis hg 405 with a maximal coaggregation rate of 1.05 mod/min, which occurred at a p. gingivalis to ...	2001	11358538
antibacterial activity of particulate bioglass against supra- and subgingival bacteria.	particulate bioglass is a bioactive material used in the repair of periodontal defects. this material undergoes a series of surface reactions in an aqueous environment which lead to osseointegration. the aim of this study was to determine whether these reactions exerted an antibacterial effect on a range of oral bacteria. streptococcus sanguis, streptococcus mutans and actinomyces viscosus were suspended in nutrient broth (nb), artificial saliva (as) or dulbecco's modified eagle medium plus 10% ...	2001	11374470
smoking affects the subgingival microflora in periodontitis.	tobacco smoking has been identified as one major risk factor for destructive periodontal disease. scaling and root planing have been shown to be less effective in smokers with periodontitis. the aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers.	2001	11394403
change in subgingival microbial profiles in adult periodontitis subjects receiving either systemically-administered amoxicillin or metronidazole.	the current investigation evaluated changes in levels and proportions of 40 bacterial species in subgingival plaque samples during, immediately after and up to 1 year after metronidazole or amoxicillin therapy combined with srp.	2001	11422580
untreated periodontal disease in indonesian adolescents. subgingival microbiota in relation to experienced progression of periodontitis.	in an indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. at baseline the age ranged from 15 to 25 years. clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up.	2001	11422582
mutans streptococci in subgingival plaque of treated and untreated patients with periodontitis.	the etiology of root caries is thought to be identical to coronal caries, though root caries seem to be more complicated because of the higher susceptibility of exposed roots (dentin) by periodontal therapy to demineralization than intact enamel. this implies that mutans streptococci are the most likely pathogens in the development of root caries. although it is known that both the numbers of mutans streptococci and the frequency of isolation in root caries lesions are negatively correlated with ...	2001	11422591
[clinical and microbiological study of adult periodontal disease].	the aim of this study was to carry out a microbiological evaluation of sites with and without clinical evidence of moderate and severe periodontitis and their correlation with clinical parameters. a total of 52 disease sites and 10 healthy sites were selected according to clinical criteria. the following clinical indexes were measured for all the sites: plaque index, gingival index, blood on probing, depth on probing and insertion level. samples of subgingival plaque were collected for culture a ...	2001	11594003
risk factors for anaerobic bloodstream infections in bone marrow transplant recipients.	the incidence of anaerobic bloodstream infections (bsi) in patients who underwent bone marrow transplantation (bmt) recently increased at our institution. a retrospective case-control study of patients undergoing bmt from january 1995 through december 1998 was performed to determine the microbiological characteristics, epidemiology, and outcome of anaerobic bsi and to identify independent risk factors for infection. anaerobic bsi occurred in 23 patients, for a rate of 4 bsis per 100 bmt procedur ...	2001	11438899
antimicrobial effect of acidified nitrite on periodontal bacteria.	the antimicrobial agent nitric oxide (no) is formed in the mouth and its concentration is directly related to salivary nitrite, which in turn is related to dietary nitrate intake. the aim of this study was to determine whether nitrite under acidic conditions will have an inhibitory effect, possibly occurring through no production, on the periodontal disease pathogens fusobacterium nucleatum, eikenella corrodens and porphyromonas gingivalis. whereas the growth of these organisms was inhibited by ...	2001	11442852
evaluation of co-aggregation among streptococcus mitis, fusobacterium nucleatum and porphyromonas gingivalis.	to develop a semi-quantitative method for evaluating co-aggregation reactions among three bacterial species, and to examine the influence of fusobacterium nucleatum on the adherence of porphyromonas gingivalis.	2001	11472519
an in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.	the bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.	2001	11482142
antibacterial properties of electron beam-sterilized gutta-percha cones.	the purpose of the study was to determine the effect of electron beam sterilization on gutta-percha cones (gpcs) at different times after sterilization. an agar diffusion test was used with -one aerobic bacterium (bacillus subtilis) and five oral anaerobic bacteria (fusobacterium nucleatum, peptostreptococcus micros, porphyromonas gingivalis, propionibacterium acnes, and veillonella parvula). with each microorganism 30 agar plates were prepared, evenly distributed among three groups (group 1: un ...	2001	11487145
metallic cough and pyogenic liver abscess.	the curious symptom of a metallic cough in association with a pyogenic hepatic abscess should heighten awareness of a fistula. we describe a 78-year-old female with severe diverticular disease, on long-term steroid treatment for polymyalgia rheumatica. she developed a pyogenic liver abscess, treated initially by antimicrobial therapy, and subsequently drained by ultrasound and computed tomography-guided percutaneous transhepatic pigtail catheterization. this was complicated by a fistulous commun ...	2001	11507364
the sensitivity of porphyromonas gingivalis and fusobacterium nucleatum to different (pseudo)halide-peroxidase combinations compared with mutans streptococci.	previous studies have shown that the peroxidase system with iodide is particularly effective against actinobacillus actinomycetemcomitans. in the present study, the effects of iodide, chloride and thiocyanate in combinations with lactoperoxidase (lp) and myeloperoxidase (mp) on the viability of porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and s. rattus were analysed. bacteria were incubated in buffer solution containing peroxidase, substrate(s) and h2o2 (all in oral ph ...	2001	11192504
fusobacterium nucleatum hepatic abscess with pylephlebitis associated with idiopathic cd4(+) t lymphocytopenia.		2001	11170936
comparison of the hydrophobic properties of candida albicans and candida dubliniensis.	although candida dubliniensis is a close genetic relative of candida albicans, it colonizes and infects fewer sites. nearly all instances of candidiasis caused by c. dubliniensis are restricted to the oral cavity. as cell surface hydrophobicity (csh) influences virulence of c. albicans, csh properties of c. dubliniensis were investigated and compared to c. albicans. growth temperature is one factor which affects the csh status of stationary-phase c. albicans. however, c. dubliniensis, similar to ...	2001	11159968
two cases of diskitis attributable to anaerobic bacteria in children.	diskitis, an inflammation of the intervertebral disk, is generally attributable to staphylococcus aureus and rarely staphylococcus epidermidis, kingella kingae, enterobacteriaciae, and streptococcus pneumoniae. in many cases, no bacterial growth is obtained from infected intervertebral discs. although anaerobic bacteria were recovered from adults with spondylodiscitis, these organisms were not reported before from children. the recovery of anaerobic bacteria in 2 children with diskitis is report ...	2001	11158500
septic arthritis of the hip due to fusobacterium nucleatum.	anaerobic bacteria are uncommon pathogens in septic arthritis. we report a case of pyarthrosis of the right hip caused by fusobacterium nucleatum, following a transient synovitis in an otherwise healthy boy. there are only a few cases involving this species described in the literature. this report illustrates the difficulty of isolating this unusual organism and emphasises the usefulness of the bactec blood culture bottles for the recovery of anaerobic bacteria.	2001	11434480
interleukin-6 deficiency increases inflammatory bone destruction.	periapical bone destruction occurs as a consequence of pulpal infection. in previous studies, we showed that interleukin-1 (il-1) is the primary stimulator of bone destruction in this model. il-6 is a pleiotropic cytokine that is induced in these infections and has both pro- and anti-inflammatory activities. in the present study, we determined the role of il-6 in regulating il-1 expression and bone resorption. the first molars of il-6 knockouts (il-6(-/-)) and wild-type mice were subjected to su ...	2001	11159963
microflora in oral ecosystems in primary sjögren's syndrome.	knowledge of the effect of primary sjögren's syndrome (pss) on the microbial flora in the different predilection sites for oral disorders is needed for planning preventive treatment. we carried out microbial analysis of samples from the dorsum of the tongue, smooth mucosa, supragingival tooth surfaces, and the gingival crevice region of 20 patients with pss.	2001	11361180
spatial arrangements and associative behavior of species in an in vitro oral biofilm model.	the spatial arrangements and associative behavior of actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis strains in an in vitro model of supragingival plaque were determined. using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distribution of the five strains were assessed during biofilm formation. the volume-derived cell numbers of each strain correlated w ...	2001	11229930
effect of lipopolysaccharide from periodontal pathogens on the production of tissue plasminogen activator and plasminogen activator inhibitor 2 by human gingival fibroblasts.	both tissue plasminogen activator (t-pa) and plasminogen activator inhibitor 2 (pai-2) are important proteolysis factors present in inflamed human periodontal tissues. the aim of the present study was to investigate the effect of lipopolysaccharide (lps) on the synthesis of t-pa and pai-2 by human gingival fibroblasts (hgf). lps from different periodontal pathogens including actinobacillus actinomycetemcomitans, porphyromonas gingivalis and fusobacterium nucleatum were extracted by the hot pheno ...	2001	11246701
new assay for measuring cell surface hydrophobicities of candida dubliniensis and candida albicans.	hydrophobic interactions, based on cell surface hydrophobicity (csh), are among the many and varied mechanisms of adherence deployed by the pathogenic yeast candida albicans. recently it was shown that, unlike c. albicans, c. dubliniensis is a species that exhibits an outer fibrillar layer consistent with constant csh. previously, c. dubliniensis grown at 25 or 37 degrees c was shown to coaggregate with the oral anaerobic bacterium fusobacterium nucleatum. c. albicans, however, demonstrated simi ...	2001	11329462
serum phenytoin concentration and igg antibody titre to periodontal bacteria in patients with phenytoin-induced gingival overgrowth.	epileptic patients taking phenytoin with gingival-overgrowth and those without gingival-overgrowth were compared for daily drug dose, plasma total phenytoin concentration, plasma free-phenytoin concentration and serum igg antibody titre against 13 periodontal bacteria. significantly higher daily drug dose was noted in patients with gingival overgrowth (p < 0.05) when compared with those without overgrowth. in addition, both total and free forms of plasma phenytoin concentration were significantl ...	2001	12666977